Rh4BG127400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012165
Physical Location & Seq
Forward (+)
21322045 .. 21326943
4899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG127400.1

Sequence Viewer

Length: 1644 bp
ATGGTGCAGTTGATGAAATCCGGCGATCTCCGCCAAACGACGCCGTGTAAGGATAAGCTTCCGGTGAAATTGGAGATCGAAGACTCCTTGGAGGAGGAGCATGGCCCCCTCAACAAGCGCTCCAAACCCTCCCCTGCTTTTCATGAGAGTTCGGGCGATAACGGGTTTGCTGTCTCAGCTCCCCAATACAATCCACTTGATGAGCCTAGCCCTTTGGGTTTGAGGCTCAGGAAGAGCCCGTCTTTATTGGAATTAATTCAAATGAAGCTTGCTCAGGGGGGTCCTCCTGCAGCGGGAGATGTACCAAGAGAAAATCTTAACTCTGGAGTTAAGAAGGAGATTAAAGCAAATGCTGCGGTGGGGGCTACTGATAAGCTTAAAGCGTCAAATTTTCCAGCTTCAATGTTAAGGATCGGTAGCTGGGAGTACAAATCAAGGTACGAGGGTGATTTAGTGGCAAAGTGTTATTTTGCAAAGCATAAGCTCGTATGGGAGGTTCTTGAAGGAGGCCTCAAGAGCAAAATAGAAATCCAGTGGTCAGATATCATGGCTCTGAAAGCAAACTGTCCTGATGATGGACCTGGTACTTTGAATGTTGTGTTGGCTAGACAACCTCTGTTCTTCAGGGAGACTAATCCTCAGCCTAGAAAGCATACTTTATGGCAAGCAACCTCAGATTTTACTGATGGGCAAGCAAGCCTACATAGGCAACATTTTCTGCAATGTCCACAAGGACTGTTAAACAAGCATTTTGAAAAGCTTATCCAGTGTGACACACGTCTTTGCTTCTTAAGTCGACAGCCAGAGATCGTTTTGGATTCACCTTATTTTGAACTGCGGCCTTCTGTATTTGAAGACCAAGGAGAATCCAAAGGCCTTGGTTTTGACCAACCGGCAAGTGGTAAAGGATCCTCCATTTCTGGTTTCCAGGATATAGCATCACCATCTGCAAGTCAGTCATCATCTTTGAAGACAGACCAGCAGGATTCAGCTGGTATGACATTAGAGCATCGGTCCAGAGAAGCTCCTTCCCCTAGCTCAGTTATGGATGCTCGTGCAATGGAAGGAAATGGAAGTTCTGAAGTTGTTGATTCCAGGGGGCCAAGAAACTGGGAACAGATAAAAGTGCCTGGGCTCCGTCCTTCAATGTCGATGAGTGATTTTATGAGTCACATTGGGAACTGTATTTCAGAACAAATGACTTCCGGAAATCCACCTTCTGCTGATCAACAGTCGGAATACCAGGATATTCTCGAGGACATTGCGCAGTACCTGTTAAGTGACACTCAATTAACTGCAGCAGATGAGAGTCATGAGACATCTCTCATGTCCAGGGTCAATTCTCTTTGTTGCCTTTTGCAGAAGGATCCCACTTCTCTTCAGACTTCGCAGGTTGAAGTTGAAGGCACAGATGGTGGAAGGGATAGTCAGCTTAACCACACTCCTGGTGGTATATCTGGAGCTGATATGCAGGCTCCTGAAGAGGATATGAAGGATGTTCCTGGAGGAAAGAAGGCACCGGGAATGTCGAGAAAAGACTCCTTCGGGGAATTGCTGTTTCACCTCCCTCGCATCGCCTCTCTTCCCTCACTTCCAAAAGTCTTGTTCAACATTTCAGAAGATTGTGAGAGTCAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

547

Amino Acids

59.86

Weight (kDa)

5.33

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 130 - 248 9e-62 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24100 AT4G30780
fragaria_vesca FvH4_4g10180
malus_domestica MD00G1164000.v1.1 MD16G1259500.v1.1
prunus_persica Prupe.1G102300_v2.0.a1
pyrus_communis pycom13g28620 pycom16g22740
rosa_chinensis RchiOBHm_Chr4g0405561
rosa_laevigata RLG00000008836
rosa_multiflora Rmu_sc0005023.1_g000013
rosa_roxburghii Rroxscaffold_5G00350070
rosa_rugosa Rorug04G0060600 Rorug04G0060600
rosa_samantha Rh4AG132500 Rh4BG127400 Rh4CG140200 Rh4DG126800
rosa_wichuraiana Rw4G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1266
Acc36I ACCTGC 1 cut(s) 1381
AccB1I GGYRCC 1 cut(s) 1516
AccI GTMKAC 1 cut(s) 796
AccIII TCCGGA 1 cut(s) 1205
AciI CCGC 4 cut(s) 31, 293, 356, 838
AclWI GGATC 5 cut(s) 419, 903, 916, 1361, 1374
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 4 cut(s) 607, 1101, 1364, 1500
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 5 cut(s) 303, 428, 440, 586, 1271
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 3 cut(s) 293, 575, 899
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 776
AjiI CACGTC 1 cut(s) 779
AjnI CCWGG 8 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
Alw26I GTCTC 3 cut(s) 178, 623, 1310
AlwI GGATC 5 cut(s) 419, 903, 916, 1361, 1374
AlwNI CAGNNNCTG 1 cut(s) 1273
Ama87I CYCGRG 1 cut(s) 1253
Aor13HI TCCGGA 1 cut(s) 1205
Aor51HI AGCGCT 1 cut(s) 119
AoxI GGCC 5 cut(s) 103, 508, 839, 874, 1100
ApeKI GCWGC 3 cut(s) 290, 353, 1298
ApoI RAATTY 1 cut(s) 388
AseI ATTAAT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 2 cut(s) 120, 1267
AspS9I GGNCC 5 cut(s) 104, 281, 578, 1014, 1100
AsuC2I CCSGG 1 cut(s) 1521
AsuHPI GGTGA 5 cut(s) 76, 458, 813, 933, 1553
AvaI CYCGRG 1 cut(s) 1253
AvaII GGWCC 3 cut(s) 281, 578, 1014
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BamHI GGATCC 2 cut(s) 908, 1366
BanI GGYRCC 1 cut(s) 1516
BanII GRGCYC 2 cut(s) 239, 1137
BauI CACGAG 1 cut(s) 1053
BbsI GAAGAC 3 cut(s) 87, 861, 977
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 3 cut(s) 302, 340, 1310
BccI CCATC 4 cut(s) 569, 680, 952, 1406
BceAI ACGGC 1 cut(s) 28
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BciT130I CCWGG 8 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503
BclI TGATCA 1 cut(s) 1225
BcnI CCSGG 1 cut(s) 1521
BcoDI GTCTC 3 cut(s) 178, 623, 1310
BfaI CTAG 4 cut(s) 207, 606, 645, 1035
BfmI CTRYAG 2 cut(s) 288, 1296
BfoI RGCGCY 1 cut(s) 121
BfrI CTTAAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 1381
BisI GCNGC 4 cut(s) 291, 354, 839, 1299
BlsI GCNGC 4 cut(s) 292, 355, 840, 1300
Bme1390I CCNGG 9 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503, 1521
Bme18I GGWCC 3 cut(s) 281, 578, 1014
BmeT110I CYCGRG 1 cut(s) 1253
BmgBI CACGTC 1 cut(s) 779
BmgT120I GGNCC 5 cut(s) 104, 281, 578, 1014, 1100
BmiI GGNNCC 8 cut(s) 106, 282, 910, 1101, 1136, 1368, 1476, 1518
BmrFI CCNGG 9 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503, 1521
BmrI ACTGGG 1 cut(s) 1120
BmsI GCATC 4 cut(s) 947, 1018, 1039, 1581
BmuI ACTGGG 1 cut(s) 1120
BoxI GACNNNNGTC 1 cut(s) 777
BpiI GAAGAC 3 cut(s) 87, 861, 977
BpmI CTGGAG 3 cut(s) 345, 1479, 1524
Bpu10I CCTNAGC 3 cut(s) 227, 273, 639
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 1521
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 6 cut(s) 87, 859, 877, 1095, 1130, 1332
BsaWI WCCGGW 2 cut(s) 61, 1205
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 575, 899
Bse118I RCCGGY 1 cut(s) 892
Bse1I ACTGG 3 cut(s) 532, 766, 1115
Bse3DI GCAATG 3 cut(s) 728, 1065, 1260
BseAI TCCGGA 1 cut(s) 1205
BseBI CCWGG 8 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503
BseDI CCNNGG 6 cut(s) 87, 859, 877, 1095, 1130, 1332
BseGI GGATG 2 cut(s) 1054, 1501
BseLI CCNNNNNNNGG 3 cut(s) 293, 575, 899
BseMI GCAATG 3 cut(s) 728, 1065, 1260
BseMII CTCAG 6 cut(s) 189, 241, 287, 653, 687, 1053
BseNI ACTGG 3 cut(s) 532, 766, 1115
BseRI GAGGAG 2 cut(s) 107, 110
BseXI GCAGC 3 cut(s) 302, 340, 1310
BseYI CCCAGC 1 cut(s) 420
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 5 cut(s) 105, 510, 841, 876, 1102
BshNI GGYRCC 1 cut(s) 1516
BsiHKCI CYCGRG 1 cut(s) 1253
BsiSI CCGG 5 cut(s) 21, 62, 893, 1206, 1520
BslI CCNNNNNNNGG 3 cut(s) 293, 575, 899
BsmAI GTCTC 3 cut(s) 178, 623, 1310
BsnI GGCC 5 cut(s) 105, 510, 841, 876, 1102
BsoBI CYCGRG 1 cut(s) 1253
Bsp1286I GDGCHC 2 cut(s) 239, 1137
Bsp13I TCCGGA 1 cut(s) 1205
Bsp143I GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
BspACI CCGC 4 cut(s) 31, 293, 356, 838
BspANI GGCC 5 cut(s) 105, 510, 841, 876, 1102
BspCNI CTCAG 6 cut(s) 188, 240, 286, 652, 686, 1052
BspEI TCCGGA 1 cut(s) 1205
BspHI TCATGA 2 cut(s) 142, 1312
BspLI GGNNCC 8 cut(s) 106, 282, 910, 1101, 1136, 1368, 1476, 1518
BspMAI CTGCAG 2 cut(s) 292, 1300
BspMI ACCTGC 1 cut(s) 1381
BspPI GGATC 5 cut(s) 419, 903, 916, 1361, 1374
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 1516
BspTI CTTAAG 1 cut(s) 790
BsrDI GCAATG 3 cut(s) 728, 1065, 1260
BsrFI RCCGGY 1 cut(s) 892
BsrI ACTGG 3 cut(s) 532, 766, 1115
BssAI RCCGGY 1 cut(s) 892
BssECI CCNNGG 6 cut(s) 87, 859, 877, 1095, 1130, 1332
BssMI GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
BssNI GRCGYC 1 cut(s) 41
BssSI CACGAG 1 cut(s) 1053
BssT1I CCWWGG 3 cut(s) 87, 859, 877
Bst2BI CACGAG 1 cut(s) 1053
Bst2UI CCWGG 8 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503
Bst4CI ACNGT 4 cut(s) 566, 738, 1184, 1233
Bst6I CTCTTC 4 cut(s) 227, 1383, 1476, 1587
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 5 cut(s) 270, 666, 693, 697, 1473
BstDEI CTNAG 6 cut(s) 175, 227, 273, 639, 673, 1039
BstF5I GGATG 2 cut(s) 1054, 1501
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 1267
BstKTI GATC 7 cut(s) 28, 78, 414, 810, 911, 1228, 1369
BstMAI GTCTC 3 cut(s) 178, 623, 1310
BstMBI GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
BstMWI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
BstNI CCWGG 8 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503
BstPAI GACNNNNGTC 1 cut(s) 777
BstSCI CCNGG 9 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501, 1519
BstSFI CTRYAG 2 cut(s) 288, 1296
BstV1I GCAGC 3 cut(s) 302, 340, 1310
BstV2I GAAGAC 3 cut(s) 87, 861, 977
BstX2I RGATCY 2 cut(s) 908, 1366
BstXI CCANNNNNNTGG 2 cut(s) 1110, 1445
BstYI RGATCY 2 cut(s) 908, 1366
BsuRI GGCC 5 cut(s) 105, 510, 841, 876, 1102
BtgZI GCGATG 1 cut(s) 1558
BtrI CACGTC 1 cut(s) 779
BtsCI GGATG 2 cut(s) 1054, 1501
BtsIMutI CAGTG 2 cut(s) 539, 773
BveI ACCTGC 1 cut(s) 1381
Cac8I GCNNGC 5 cut(s) 270, 666, 693, 697, 1473
CaiI CAGNNNCTG 1 cut(s) 1273
CciI TCATGA 2 cut(s) 142, 1312
CfoI GCGC 2 cut(s) 120, 1267
Cfr10I RCCGGY 1 cut(s) 892
Cfr13I GGNCC 5 cut(s) 104, 281, 578, 1014, 1100
CseI GACGC 2 cut(s) 49, 372
CsiI ACCWGGT 1 cut(s) 580
Csp6I GTAC 5 cut(s) 302, 427, 439, 585, 1270
CviAII CATG 5 cut(s) 101, 143, 547, 1313, 1327
CviQI GTAC 5 cut(s) 302, 427, 439, 585, 1270
DdeI CTNAG 6 cut(s) 175, 227, 273, 639, 673, 1039
DpnI GATC 7 cut(s) 27, 77, 413, 809, 910, 1227, 1368
DpnII GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
Eam1104I CTCTTC 4 cut(s) 227, 1383, 1476, 1587
EarI CTCTTC 4 cut(s) 227, 1383, 1476, 1587
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 87, 859, 877
Eco147I AGGCCT 2 cut(s) 510, 876
Eco24I GRGCYC 2 cut(s) 239, 1137
Eco32I GATATC 1 cut(s) 544
Eco47I GGWCC 3 cut(s) 281, 578, 1014
Eco47III AGCGCT 1 cut(s) 119
Eco57I CTGAAG 4 cut(s) 607, 1101, 1364, 1500
Eco88I CYCGRG 1 cut(s) 1253
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 8 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 3 cut(s) 87, 859, 877
EcoT38I GRGCYC 2 cut(s) 239, 1137
ErhI CCWWGG 3 cut(s) 87, 859, 877
FaeI CATG 5 cut(s) 104, 146, 550, 1316, 1330
FatI CATG 5 cut(s) 100, 142, 546, 1312, 1326
FauI CCCGC 1 cut(s) 286
FbaI TGATCA 1 cut(s) 1225
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 4 cut(s) 291, 354, 839, 1299
FokI GGATG 2 cut(s) 1061, 1508
FriOI GRGCYC 2 cut(s) 239, 1137
Fsp4HI GCNGC 4 cut(s) 291, 354, 839, 1299
FspBI CTAG 4 cut(s) 207, 606, 645, 1035
FspI TGCGCA 1 cut(s) 1266
GlaI GCGC 2 cut(s) 119, 1266
GluI GCNGC 4 cut(s) 291, 354, 839, 1299
GsaI CCCAGC 1 cut(s) 424
GsuI CTGGAG 3 cut(s) 345, 1479, 1524
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 5 cut(s) 105, 510, 841, 876, 1102
HapII CCGG 5 cut(s) 21, 62, 893, 1206, 1520
HgaI GACGC 2 cut(s) 49, 372
HhaI GCGC 2 cut(s) 120, 1267
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 5 cut(s) 104, 146, 550, 1316, 1330
Hin6I GCGC 2 cut(s) 118, 1265
HinP1I GCGC 2 cut(s) 118, 1265
HincII GTYRAC 1 cut(s) 797
HindII GTYRAC 1 cut(s) 797
HindIII AAGCTT 4 cut(s) 56, 266, 374, 758
HinfI GANTC 9 cut(s) 83, 818, 866, 986, 1091, 1168, 1309, 1538, 1630
HpaII CCGG 5 cut(s) 21, 62, 893, 1206, 1520
HphI GGTGA 5 cut(s) 76, 458, 813, 933, 1553
Hpy166II GTNNAC 2 cut(s) 728, 797
Hpy188I TCNGA 8 cut(s) 541, 555, 676, 1081, 1192, 1237, 1383, 1618
Hpy8I GTNNAC 2 cut(s) 728, 797
Hpy99I CGWCG 1 cut(s) 43
HpyCH4III ACNGT 4 cut(s) 566, 738, 1184, 1233
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 9 cut(s) 7, 290, 473, 721, 950, 1058, 1298, 1360, 1471
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
HpyF3I CTNAG 6 cut(s) 175, 227, 273, 639, 673, 1039
HpySE526I ACGT 1 cut(s) 778
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 5 cut(s) 104, 146, 550, 1316, 1330
HspAI GCGC 2 cut(s) 118, 1265
Kpn2I TCCGGA 1 cut(s) 1205
Ksp22I TGATCA 1 cut(s) 1225
Kzo9I GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 7 cut(s) 97, 125, 184, 1030, 1140, 1460, 1480
Lsp1109I GCAGC 3 cut(s) 302, 340, 1310
LweI GCATC 4 cut(s) 947, 1018, 1039, 1581
MabI ACCWGGT 1 cut(s) 580
MaeI CTAG 4 cut(s) 207, 606, 645, 1035
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 3 cut(s) 770, 1169, 1280
MalI GATC 7 cut(s) 27, 77, 413, 809, 910, 1227, 1368
MboI GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
MboII GAAGA 9 cut(s) 92, 244, 613, 866, 982, 1370, 1493, 1574, 1631
MflI RGATCY 2 cut(s) 908, 1366
MhlI GDGCHC 2 cut(s) 239, 1137
MluCI AATT 7 cut(s) 68, 251, 255, 388, 1289, 1339, 1550
MlyI GAGTC 5 cut(s) 77, 1177, 1318, 1532, 1639
MmeI TCCRAC 1 cut(s) 1215
MroI TCCGGA 1 cut(s) 1205
MroXI GAANNNNTTC 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 293, 992
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 5 cut(s) 21, 62, 893, 1206, 1520
MspR9I CCNGG 9 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503, 1521
MvaI CCWGG 8 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503
MwoI GCNNNNNNNGC 7 cut(s) 30, 176, 353, 362, 516, 557, 649
NciI CCSGG 1 cut(s) 1521
NdeII GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
NlaIII CATG 5 cut(s) 104, 146, 550, 1316, 1330
NlaIV GGNNCC 8 cut(s) 106, 282, 910, 1101, 1136, 1368, 1476, 1518
NmuCI GTSAC 3 cut(s) 770, 1169, 1280
NsbI TGCGCA 1 cut(s) 1266
PaeR7I CTCGAG 1 cut(s) 1253
PagI TCATGA 2 cut(s) 142, 1312
PceI AGGCCT 2 cut(s) 510, 876
PciSI GCTCTTC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 4 cut(s) 818, 866, 986, 1091
PfoI TCCNGGA 2 cut(s) 927, 1501
PkrI GCNGC 4 cut(s) 292, 355, 840, 1300
PleI GAGTC 5 cut(s) 77, 1176, 1317, 1532, 1638
PpsI GAGTC 5 cut(s) 77, 1176, 1317, 1532, 1638
PpuMI RGGWCCY 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 777
PshBI ATTAAT 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 8 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501
PspFI CCCAGC 1 cut(s) 420
PspGI CCWGG 8 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501
PspN4I GGNNCC 8 cut(s) 106, 282, 910, 1101, 1136, 1368, 1476, 1518
PspPI GGNCC 5 cut(s) 104, 281, 578, 1014, 1100
PspPPI RGGWCCY 1 cut(s) 281
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstI CTGCAG 2 cut(s) 292, 1300
PstNI CAGNNNCTG 1 cut(s) 1273
PsuI RGATCY 2 cut(s) 908, 1366
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 303, 428, 440, 586, 1271
RsaNI GTAC 5 cut(s) 302, 427, 439, 585, 1270
SalI GTCGAC 1 cut(s) 795
SapI GCTCTTC 1 cut(s) 227
SatI GCNGC 4 cut(s) 291, 354, 839, 1299
Sau3AI GATC 7 cut(s) 25, 75, 411, 807, 908, 1225, 1366
Sau96I GGNCC 5 cut(s) 104, 281, 578, 1014, 1100
SchI GAGTC 5 cut(s) 77, 1177, 1318, 1532, 1639
ScrFI CCNGG 9 cut(s) 582, 929, 1096, 1131, 1244, 1333, 1446, 1503, 1521
SduI GDGCHC 2 cut(s) 239, 1137
SexAI ACCWGGT 1 cut(s) 580
SfaNI GCATC 4 cut(s) 947, 1018, 1039, 1581
SfcI CTRYAG 2 cut(s) 288, 1296
Sfr274I CTCGAG 1 cut(s) 1253
SinI GGWCC 3 cut(s) 281, 578, 1014
SlaI CTCGAG 1 cut(s) 1253
SmlI CTYRAG 3 cut(s) 512, 790, 1253
SmoI CTYRAG 3 cut(s) 512, 790, 1253
Sse9I AATT 7 cut(s) 68, 251, 255, 388, 1289, 1339, 1550
SseBI AGGCCT 2 cut(s) 510, 876
SsiI CCGC 4 cut(s) 31, 293, 356, 838
SspMI CTAG 4 cut(s) 207, 606, 645, 1035
StuI AGGCCT 2 cut(s) 510, 876
StyD4I CCNGG 9 cut(s) 580, 927, 1094, 1129, 1242, 1331, 1444, 1501, 1519
StyI CCWWGG 3 cut(s) 87, 859, 877
TaaI ACNGT 4 cut(s) 566, 738, 1184, 1233
TaiI ACGT 1 cut(s) 781
TaqI TCGA 5 cut(s) 78, 796, 1151, 1254, 1529
TaqII GACCGA 1 cut(s) 1002
TasI AATT 7 cut(s) 68, 251, 255, 388, 1289, 1339, 1550
TatI WGTACW 1 cut(s) 426
TauI GCSGC 1 cut(s) 841
TfiI GAWTC 4 cut(s) 818, 866, 986, 1091
TscAI CASTG 2 cut(s) 539, 773
TseFI GTSAC 3 cut(s) 770, 1169, 1280
TseI GCWGC 3 cut(s) 290, 353, 1298
Tsp45I GTSAC 3 cut(s) 770, 1169, 1280
TspDTI ATGAA 4 cut(s) 29, 131, 278, 1505
TspGWI ACGGA 1 cut(s) 1127
TspRI CASTG 2 cut(s) 539, 773
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 3 cut(s) 281, 578, 1014
VspI ATTAAT 1 cut(s) 254
XapI RAATTY 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1445
XhoI CTCGAG 1 cut(s) 1253
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 4 cut(s) 207, 606, 645, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.