Rw4G010730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012165
Physical Location & Seq
Forward (+)
25043111 .. 25048207
5097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010730.1

Sequence Viewer

Length: 1269 bp
ATGTTAAGGATCGGTAGCTGGGAGTACAAATCAAGGTACGAGGGTGATTTAGTGGCAAAGTGTTATTTTGCAAAGCATAAGCTCGTATGGGAGGTTCTTGAAGGAGGCCTCAAGAGTAAAATAGAAATCCAGTGGTCAGATATCATGGCCCTGAAAGCAAACTGTCCTGATGATGGACCTGGTACTTTGAATGTTGTGTTGGCTAGACAACCTCTGTTCTTCAGGGAGACTAATCCTCAGCCTAGAAAGCATACTTTATGGCAAGCAACCTCAGATTTTACTGATGGGCAAGCAAGCCTGCATAGGCAACATTTTCTGCAATGCCCACAAGGACTGTTAAACAAGCATTTTGAAAAGCTTATTCAGTGTGACACACGTCTTTGCTTCTTAAGTCGACAGCCAGAGATCGTTTTGGATTCACCTTATTTTGAACTGCGGCCTTCTGTATTTGAAGACCAAGGAGAATCCAAAGGCCTTGGTTTTGACCAACCGGCAAGTGGTAAAGGATCCTCCATTTCTGGTTTCCAGGATATAGCATCACCATCTGCAAGTCAGTCATCATCTTTGAAGGCAGACCAGCAGGATTCAGCTGGTATCACATTAGAGCATCGGTCCAGAGAAGCTCCTTCCCCTAGCTCAGGAAATGGAAGTTCTGAAGTTGTTGATTCCAGGGGGCCAAGAAACTGGGAACAGATAAAAGTGCCTGGGCTCCATCCTTCAATGTCGATGAGTGATTTTATGAGTCACATTGGGAACTGTATTTCAGAACAAATGACTTCCGGAAATCCCCCTTCTGCTGATCAACAGTCGGAATACCAGGATATTCTCGAGGACATTGCGCAGTACCTGTTAAGTGACACTCAATTAACTGCAGCAGATGAGAGTCATGAGACATCTCTCATGTCCAGGGTCAATTCTCTTTGTTGCCTTTTGCAGAAGGACCCCACTTCTCTTCAGACTTCGCAGGTAGAAGTTGAAGGCACAGATGGTGGAAGGGATATTCAGCTTAACCACACTCCTGGTGGTATATCTGGAGCTGATATGCAGGCTCCTGAAGAGGATATGAAGGATGTTCCTGGAGGAAAGAAGGCACCGGGAATGTCAGAAAAGACTCCTTCGGGGAATTGCTGTTTCACCTCCCTCGCATCGCCTCTCTTCCCTCACTTCCAAAAGTGTTGTTCAACATTTCAGAAGATTGTGAGAGTCAAGGTAGATAAGTCATTTTTTCCAAAAAAATCAAATCCTATAAGTCTACAGCATGCGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

46.43

Weight (kDa)

5.58

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_TRF2_HOY1 PF24818 1 - 114 1.4e-59 TRF2/HOY1 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24100 AT4G30780
fragaria_vesca FvH4_4g10180
malus_domestica MD00G1164000.v1.1 MD16G1259500.v1.1
prunus_persica Prupe.1G102300_v2.0.a1
pyrus_communis pycom13g28620 pycom16g22740
rosa_chinensis RchiOBHm_Chr4g0405561
rosa_laevigata RLG00000008836
rosa_multiflora Rmu_sc0005023.1_g000013
rosa_roxburghii Rroxscaffold_5G00350070
rosa_rugosa Rorug04G0060600 Rorug04G0060600
rosa_samantha Rh4AG132500 Rh4BG127400 Rh4CG140200 Rh4DG126800
rosa_wichuraiana Rw4G010730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 840
Acc36I ACCTGC 1 cut(s) 955
AccB1I GGYRCC 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 394, 1252
AccIII TCCGGA 1 cut(s) 779
AciI CCGC 1 cut(s) 436
AclWI GGATC 3 cut(s) 17, 501, 514
AcuI CTGAAG 4 cut(s) 205, 675, 938, 1074
AfaI GTAC 4 cut(s) 26, 38, 184, 845
AfiI CCNNNNNNNGG 3 cut(s) 173, 497, 638
AflII CTTAAG 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 9 cut(s) 101, 190, 353, 431, 452, 568, 720, 977, 1182
AjiI CACGTC 1 cut(s) 377
AjnI CCWGG 8 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 8 cut(s) 18, 82, 358, 590, 623, 636, 1006, 1037
AluI AGCT 8 cut(s) 18, 82, 358, 590, 623, 636, 1006, 1037
Alw26I GTCTC 2 cut(s) 221, 884
AlwI GGATC 3 cut(s) 17, 501, 514
AlwNI CAGNNNCTG 1 cut(s) 847
Ama87I CYCGRG 1 cut(s) 827
Aor13HI TCCGGA 1 cut(s) 779
AoxI GGCC 5 cut(s) 106, 147, 437, 472, 674
ApeKI GCWGC 1 cut(s) 872
AspLEI GCGC 1 cut(s) 841
AspS9I GGNCC 5 cut(s) 148, 176, 612, 674, 940
AsuC2I CCSGG 1 cut(s) 1095
AsuHPI GGTGA 4 cut(s) 56, 411, 531, 1126
AvaI CYCGRG 1 cut(s) 827
AvaII GGWCC 3 cut(s) 176, 612, 940
BaeI ACNNNNGTAYC 2 cut(s) 28, 61
BamHI GGATCC 1 cut(s) 506
BanI GGYRCC 1 cut(s) 1090
BanII GRGCYC 1 cut(s) 711
BbsI GAAGAC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 884
BccI CCATC 5 cut(s) 167, 278, 550, 720, 980
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 8 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 1095
BcoDI GTCTC 2 cut(s) 221, 884
BfaI CTAG 3 cut(s) 204, 243, 633
BfmI CTRYAG 2 cut(s) 870, 1253
BfrI CTTAAG 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 955
BisI GCNGC 2 cut(s) 437, 873
BlsI GCNGC 2 cut(s) 438, 874
Bme1390I CCNGG 9 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077, 1095
Bme18I GGWCC 3 cut(s) 176, 612, 940
BmeT110I CYCGRG 1 cut(s) 827
BmgBI CACGTC 1 cut(s) 377
BmgT120I GGNCC 5 cut(s) 148, 176, 612, 674, 940
BmiI GGNNCC 6 cut(s) 508, 675, 710, 942, 1050, 1092
BmrFI CCNGG 9 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077, 1095
BmrI ACTGGG 1 cut(s) 694
BmsI GCATC 3 cut(s) 545, 616, 1154
BmuI ACTGGG 1 cut(s) 694
BoxI GACNNNNGTC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 459
BpmI CTGGAG 2 cut(s) 1053, 1098
Bpu10I CCTNAGC 2 cut(s) 237, 637
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 1095
BsaJI CCNNGG 5 cut(s) 457, 475, 669, 704, 906
BsaWI WCCGGW 1 cut(s) 779
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 497, 638
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 130, 689
Bse3DI GCAATG 2 cut(s) 326, 834
BseAI TCCGGA 1 cut(s) 779
BseBI CCWGG 8 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077
BseDI CCNNGG 5 cut(s) 457, 475, 669, 704, 906
BseGI GGATG 2 cut(s) 712, 1075
BseLI CCNNNNNNNGG 3 cut(s) 173, 497, 638
BseMI GCAATG 2 cut(s) 326, 834
BseMII CTCAG 3 cut(s) 251, 285, 651
BseNI ACTGG 2 cut(s) 130, 689
BseXI GCAGC 1 cut(s) 884
BseYI CCCAGC 1 cut(s) 18
BshFI GGCC 5 cut(s) 108, 149, 439, 474, 676
BshNI GGYRCC 1 cut(s) 1090
BsiHKCI CYCGRG 1 cut(s) 827
BsiSI CCGG 3 cut(s) 491, 780, 1094
BslI CCNNNNNNNGG 3 cut(s) 173, 497, 638
BsmAI GTCTC 2 cut(s) 221, 884
BsnI GGCC 5 cut(s) 108, 149, 439, 474, 676
BsoBI CYCGRG 1 cut(s) 827
Bsp1286I GDGCHC 1 cut(s) 711
Bsp13I TCCGGA 1 cut(s) 779
Bsp143I GATC 4 cut(s) 9, 405, 506, 799
BspACI CCGC 1 cut(s) 436
BspANI GGCC 5 cut(s) 108, 149, 439, 474, 676
BspCNI CTCAG 3 cut(s) 250, 284, 650
BspEI TCCGGA 1 cut(s) 779
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 6 cut(s) 508, 675, 710, 942, 1050, 1092
BspMAI CTGCAG 1 cut(s) 874
BspMI ACCTGC 1 cut(s) 955
BspPI GGATC 3 cut(s) 17, 501, 514
BspT107I GGYRCC 1 cut(s) 1090
BspTI CTTAAG 1 cut(s) 388
BsrDI GCAATG 2 cut(s) 326, 834
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 2 cut(s) 130, 689
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 5 cut(s) 457, 475, 669, 704, 906
BssMI GATC 4 cut(s) 9, 405, 506, 799
BssT1I CCWWGG 2 cut(s) 457, 475
Bst2UI CCWGG 8 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077
Bst4CI ACNGT 4 cut(s) 164, 336, 758, 807
Bst6I CTCTTC 3 cut(s) 957, 1050, 1160
BstAFI CTTAAG 1 cut(s) 388
BstC8I GCNNGC 6 cut(s) 264, 291, 295, 299, 1047, 1260
BstDEI CTNAG 3 cut(s) 237, 271, 637
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 2 cut(s) 712, 1075
BstHHI GCGC 1 cut(s) 841
BstKTI GATC 4 cut(s) 12, 408, 509, 802
BstMAI GTCTC 2 cut(s) 221, 884
BstMBI GATC 4 cut(s) 9, 405, 506, 799
BstMWI GCNNNNNNNGC 2 cut(s) 155, 247
BstNI CCWGG 8 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077
BstNSI RCATGY 1 cut(s) 1262
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 9 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075, 1093
BstSFI CTRYAG 2 cut(s) 870, 1253
BstV1I GCAGC 1 cut(s) 884
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 506
BstXI CCANNNNNNTGG 2 cut(s) 684, 1019
BstYI RGATCY 1 cut(s) 506
BsuRI GGCC 5 cut(s) 108, 149, 439, 474, 676
BtgZI GCGATG 1 cut(s) 1131
BtrI CACGTC 1 cut(s) 377
BtsCI GGATG 2 cut(s) 712, 1075
BtsIMutI CAGTG 2 cut(s) 137, 371
BveI ACCTGC 1 cut(s) 955
Cac8I GCNNGC 6 cut(s) 264, 291, 295, 299, 1047, 1260
CaiI CAGNNNCTG 1 cut(s) 847
CciI TCATGA 1 cut(s) 886
CfoI GCGC 1 cut(s) 841
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 5 cut(s) 148, 176, 612, 674, 940
CsiI ACCWGGT 1 cut(s) 178
Csp6I GTAC 4 cut(s) 25, 37, 183, 844
CviAII CATG 4 cut(s) 145, 887, 901, 1259
CviQI GTAC 4 cut(s) 25, 37, 183, 844
DdeI CTNAG 3 cut(s) 237, 271, 637
DpnI GATC 4 cut(s) 11, 407, 508, 801
DpnII GATC 4 cut(s) 9, 405, 506, 799
Eam1104I CTCTTC 3 cut(s) 957, 1050, 1160
EarI CTCTTC 3 cut(s) 957, 1050, 1160
Eco130I CCWWGG 2 cut(s) 457, 475
Eco147I AGGCCT 2 cut(s) 108, 474
Eco24I GRGCYC 1 cut(s) 711
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 176, 612, 940
Eco57I CTGAAG 4 cut(s) 205, 675, 938, 1074
Eco88I CYCGRG 1 cut(s) 827
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 940
EcoRII CCWGG 8 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 457, 475
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 2 cut(s) 457, 475
FaeI CATG 4 cut(s) 148, 890, 904, 1262
FatI CATG 4 cut(s) 144, 886, 900, 1258
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 2 cut(s) 394, 1252
Fnu4HI GCNGC 2 cut(s) 437, 873
FokI GGATG 2 cut(s) 699, 1082
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 2 cut(s) 437, 873
FspBI CTAG 3 cut(s) 204, 243, 633
FspI TGCGCA 1 cut(s) 840
GlaI GCGC 1 cut(s) 840
GluI GCNGC 2 cut(s) 437, 873
GsaI CCCAGC 1 cut(s) 22
GsuI CTGGAG 2 cut(s) 1053, 1098
HaeIII GGCC 5 cut(s) 108, 149, 439, 474, 676
HapII CCGG 3 cut(s) 491, 780, 1094
HhaI GCGC 1 cut(s) 841
Hin1II CATG 4 cut(s) 148, 890, 904, 1262
Hin6I GCGC 1 cut(s) 839
HinP1I GCGC 1 cut(s) 839
HincII GTYRAC 1 cut(s) 395
HindII GTYRAC 1 cut(s) 395
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 8 cut(s) 416, 464, 584, 665, 742, 883, 1111, 1203
HpaII CCGG 3 cut(s) 491, 780, 1094
HphI GGTGA 4 cut(s) 56, 411, 531, 1126
Hpy166II GTNNAC 2 cut(s) 395, 1253
Hpy188I TCNGA 8 cut(s) 139, 274, 655, 766, 811, 957, 1105, 1191
Hpy8I GTNNAC 2 cut(s) 395, 1253
HpyCH4III ACNGT 4 cut(s) 164, 336, 758, 807
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 7 cut(s) 71, 301, 319, 548, 872, 934, 1045
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 247
HpyF3I CTNAG 3 cut(s) 237, 271, 637
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 4 cut(s) 148, 890, 904, 1262
HspAI GCGC 1 cut(s) 839
Kpn2I TCCGGA 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 4 cut(s) 9, 405, 506, 799
LmnI GCTCC 4 cut(s) 628, 714, 1034, 1054
Lsp1109I GCAGC 1 cut(s) 884
LweI GCATC 3 cut(s) 545, 616, 1154
MabI ACCWGGT 1 cut(s) 178
MaeI CTAG 3 cut(s) 204, 243, 633
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 3 cut(s) 368, 743, 854
MalI GATC 4 cut(s) 11, 407, 508, 801
MboI GATC 4 cut(s) 9, 405, 506, 799
MboII GAAGA 6 cut(s) 211, 464, 944, 1067, 1147, 1204
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 3 cut(s) 863, 913, 1123
MlyI GAGTC 4 cut(s) 751, 892, 1105, 1212
MmeI TCCRAC 1 cut(s) 789
MroI TCCGGA 1 cut(s) 779
MseI TTAA 6 cut(s) 5, 338, 389, 851, 866, 1008
MspA1I CMGCKG 1 cut(s) 590
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 3 cut(s) 491, 780, 1094
MspR9I CCNGG 9 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077, 1095
MvaI CCWGG 8 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077
MwoI GCNNNNNNNGC 2 cut(s) 155, 247
NciI CCSGG 1 cut(s) 1095
NdeII GATC 4 cut(s) 9, 405, 506, 799
NlaIII CATG 4 cut(s) 148, 890, 904, 1262
NlaIV GGNNCC 6 cut(s) 508, 675, 710, 942, 1050, 1092
NmuCI GTSAC 3 cut(s) 368, 743, 854
NsbI TGCGCA 1 cut(s) 840
NspI RCATGY 1 cut(s) 1262
PaeI GCATGC 1 cut(s) 1262
PaeR7I CTCGAG 1 cut(s) 827
PagI TCATGA 1 cut(s) 886
PceI AGGCCT 2 cut(s) 108, 474
PfeI GAWTC 4 cut(s) 416, 464, 584, 665
PfoI TCCNGGA 2 cut(s) 525, 1075
PkrI GCNGC 2 cut(s) 438, 874
PleI GAGTC 4 cut(s) 750, 891, 1105, 1211
PpsI GAGTC 4 cut(s) 750, 891, 1105, 1211
PpuMI RGGWCCY 1 cut(s) 940
PshAI GACNNNNGTC 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 940
Psp6I CCWGG 8 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075
PspFI CCCAGC 1 cut(s) 18
PspGI CCWGG 8 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075
PspN4I GGNNCC 6 cut(s) 508, 675, 710, 942, 1050, 1092
PspPI GGNCC 5 cut(s) 148, 176, 612, 674, 940
PspPPI RGGWCCY 1 cut(s) 940
PsrI GAACNNNNNNTAC 2 cut(s) 78, 110
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 4 cut(s) 26, 38, 184, 845
RsaNI GTAC 4 cut(s) 25, 37, 183, 844
SalI GTCGAC 1 cut(s) 393
SaqAI TTAA 6 cut(s) 5, 338, 389, 851, 866, 1008
SatI GCNGC 2 cut(s) 437, 873
Sau3AI GATC 4 cut(s) 9, 405, 506, 799
Sau96I GGNCC 5 cut(s) 148, 176, 612, 674, 940
SchI GAGTC 4 cut(s) 751, 892, 1105, 1212
ScrFI CCNGG 9 cut(s) 180, 527, 670, 705, 818, 907, 1020, 1077, 1095
SduI GDGCHC 1 cut(s) 711
SexAI ACCWGGT 1 cut(s) 178
SfaNI GCATC 3 cut(s) 545, 616, 1154
SfcI CTRYAG 2 cut(s) 870, 1253
Sfr274I CTCGAG 1 cut(s) 827
SinI GGWCC 3 cut(s) 176, 612, 940
SlaI CTCGAG 1 cut(s) 827
SmlI CTYRAG 3 cut(s) 110, 388, 827
SmoI CTYRAG 3 cut(s) 110, 388, 827
SphI GCATGC 1 cut(s) 1262
Sse9I AATT 3 cut(s) 863, 913, 1123
SseBI AGGCCT 2 cut(s) 108, 474
SsiI CCGC 1 cut(s) 436
SspMI CTAG 3 cut(s) 204, 243, 633
StuI AGGCCT 2 cut(s) 108, 474
StyD4I CCNGG 9 cut(s) 178, 525, 668, 703, 816, 905, 1018, 1075, 1093
StyI CCWWGG 2 cut(s) 457, 475
TaaI ACNGT 4 cut(s) 164, 336, 758, 807
TaiI ACGT 1 cut(s) 379
TaqI TCGA 3 cut(s) 394, 725, 828
TaqII GACCGA 1 cut(s) 600
TasI AATT 3 cut(s) 863, 913, 1123
TatI WGTACW 1 cut(s) 24
TauI GCSGC 1 cut(s) 439
TfiI GAWTC 4 cut(s) 416, 464, 584, 665
Tru1I TTAA 6 cut(s) 5, 338, 389, 851, 866, 1008
Tru9I TTAA 6 cut(s) 5, 338, 389, 851, 866, 1008
TscAI CASTG 2 cut(s) 137, 371
TseFI GTSAC 3 cut(s) 368, 743, 854
TseI GCWGC 1 cut(s) 872
Tsp45I GTSAC 3 cut(s) 368, 743, 854
TspDTI ATGAA 1 cut(s) 1079
TspRI CASTG 2 cut(s) 137, 371
Vha464I CTTAAG 1 cut(s) 388
VpaK11BI GGWCC 3 cut(s) 176, 612, 940
XagI CCTNNNNNAGG 1 cut(s) 636
XceI RCATGY 1 cut(s) 1262
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 1019
XhoI CTCGAG 1 cut(s) 827
XmiI GTMKAC 2 cut(s) 394, 1252
XspI CTAG 3 cut(s) 204, 243, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.