Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008593

Physical Location & Seq

Genomic Coordinates Forward (+)

38453535 .. 38457416

Gene Structure 3882 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1282200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGAGGCCTCAAATCCGGCCTCCCGTTCGGACTTCTACGCATATCAGCTGCAAGCAAGCCTCAGAAAGCGAGACTTACTTGCCGGAAAATCAATCCATGCCCAGGTCATCAAATCCGGCCTTCACCTCTGCAGTGTGTTTTTGATGAACAACCTCATGACCGTTTACCTCAAAACCGGCTCTGCTTCCGATGCCCACCGCGTGTTCGACGAAATGCCCCTAAGGAATACACTCTCATGGAACACTATTCTCTCCGCTTATGCAAAGCAGGGAAGGTTTGACGAAGCACTGAGCGTTTTCAACGCAATGCCACAACAGGACTCCGTTTCCTGGACCGCAATGATAGTGGGTTACAATCAGATGGGCCGTTACGGGAATGCCATTCAGATGTTTGTGCAGATGATTGCGGATGGAGTTCCCGTGACCCAGTTCACCCTTACTAACATTCTTTCCGCATGTGGTGCTGTTAAGGCTTTGGAGATTGGTAGAAAGGTTCATTCGTTTGTTGTTAAACTTGCGGTTGGCGGTTATGTTTCTGTTGCCAACTGTCTTTTAAACATGTATGCAAAATCTGGTGATCCCAGGACCGCGAAGATTGTTTTCGATAGGATGAGGTTGAAAAACACATCGAGCTGGAATGCTATGATTTCTTTGCATATGCAATGCGGTTGTGCTGACCTTGCTCTTGCTCAGTTTAAGCAAATGAAGAAGCACGACATTGTTTCTTGGAATTCGTTGATTGCAGGATACAATCAGCATGGCCTTGACATGGAAGCACTGACTACCTTCTCATGTATGCTTCAGGATTCTGTTCTACAGCCCGACAAGTTCACCTTAGCAAGCATTCTGTCAGCTTGTGCTAACCTTGAGAAGTTGGAACTTGGGAAGCAAATCCATGCCCATATTGTAAGAGCTGAGCTGGGCACATACGATGCGGTGAAAAATGCTTTGATCACAATGTATGCTAAATCTGGTGGGTTGGCAATTGCTCAAAAGATTTTAGAGCAAAGCGCGACTTCCGATCTCAATGTTATAGCTTTTACCGCCCTATTGCATGGGTACATAAAGCTTGGGGATGTAATGCCAGCCAGGCAGATATTTGACTCATTGAGAGACCGTGATGTGGTTGCATGGACAGCAATGATTGTTGGTTATGTACAGAATGGGTTGAATAATGATGCTTTGGAGCTCTTCATGTCAATGATAAAACAAGGCCCCAAGCCAAACAGCTACACCTTAGCAGCTATGTTAAGTGTCAGTTCGAGCTTGGCTTCTTTAGACTATGGAAAACAAATTCATGCAAGTGCCATGAGGTTGGGGGAAGTGCATTCAGTTTCTGTAAGCAATGCTCTAATTTCAATGTATTCCAAAGCTGGAAGCATCGGTGGTGCAAAGAAAGTATTCAATTTGATTTACTTGAATAGAGATACGGTTTCTTGGACTTCCATGATCATAGCCCTGGCTCAACATGGTCACGGGGAAGATGCCATTGAACTGTTTGAGAAGATGCTGTCACATGGTATTAAGCCTGACCATATAACTTATGTCGGCGTGCTCTCTGCCTGTATACATGTGGGATTGGTAGAACAGGGCAGGAGCTACTATAATTTAATGAGGAACGTCCACAAAATCGAACCCACCCATAGCCATTATGCATGCATGATTGATCTGCTTGGGCGCGCTGGATTGCTTCAAGAAGCATTCAATTTTATACAAAGTTTGCCAGTTGAACCAGATGTTATAGCTTGGGGTTCACTTTTATCTTGTTGTAAGGTTCATAAAAATGTAGATCTAGCAAAGGTTGCAGCTGAAAGATTGCTGCTTATTGATCCAGACAACAGTGGGGCCTACTCAGCCCTTGCTAATCTGTATTCAGCTTGTGGAAGATGGGAAGAAGCTGCACAAGTTAGAAGGTCCATGAAGAATAAGGGAGTGAAGAAAGAACAAGGATTTAGTTGGATTCAGATTCAAAACAAAGTTCATGTGTTTGGGGTTGATGATGGAGTTCACACGCAGAAAGAGGCAATCTACAAGATGATGGAAAAGATATGGAAAGAGATTAAAAATATGGGTTTTGTTCCCGACACTGACTCAGTATTGCATGATGTTGGTGAAGAAGTGAAGGAGAAGATGCTTAAACATCACAGTGAGAAACTTGCCATTGCATTTGGGCTAATAAATACTCCAGAGAACACGACGTTGAGGATCATGAAGAACCTTAGAGTTTGTAATGATTGTCATTCTGCCATCAAATTTATTTCCAAGCTTGTGGGCAGAGAAATCATTTTGAGAGACGCTACTCGTTTTCACCATTTTAAGAGCGGGCAGTGTTCTTGTCGGGACTATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

784

Amino Acids

86.81

Weight (kDa)

8.82

Isoelectric Point (pI)

35.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	76 - 105	1.1e-06	PPR repeat family
PPR	PF01535	79 - 104	8.2e-07	PPR repeat
PPR_2	PF13041	107 - 153	4e-06	PPR repeat family
PPR_2	PF13041	373 - 417	2.4e-10	PPR repeat family
PPR	PF01535	376 - 405	8.8e-06	PPR repeat
PPR_2	PF13041	475 - 523	4.4e-12	PPR repeat family
PPR	PF01535	478 - 508	4.7e-08	PPR repeat
E_motif	PF20431	593 - 655	7.3e-18	E motif
DYW_deaminase	PF14432	691 - 783	2.6e-43	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008593)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22070
fragaria_vesca	FvH4_6g24010 FvH4_6g24010
malus_domestica	MD01G1046800.v1.1 MD16G1282200.v1.1
prunus_persica	Prupe.3G166700_v2.0.a1
pyrus_communis	pycom01g07270
rosa_chinensis	RchiOBHm_Chr2g0115941
rosa_laevigata	RLG00000018225
rosa_multiflora	Rmu_co8514103.1_g000001 Rmu_sc0025044.1_g000002 Rmu_ssc0000197.1_g000005
rosa_roxburghii	Rroxscaffold_2G00127720 Rroxscaffold_2G00128490
rosa_rugosa	Rorug02G0201400 Rorug02G0201500 Rorug02G0201600 Rorug02G0201700
rosa_samantha	Rh2AG258200 Rh2BG269300 Rh2CG264900 Rh2DG266600
rosa_wichuraiana	Rw2G020250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	2322
AccI	GTMKAC	1 cut(s)	1567
AccII	CGCG	4 cut(s)	201, 590, 1013, 1680
AclWI	GGATC	3 cut(s)	572, 1823, 2213
AcsI	RAATTY	3 cut(s)	730, 1293, 2252
AcuI	CTGAAG	1 cut(s)	785
AdeI	CACNNNGTG	1 cut(s)	202
AfaI	GTAC	2 cut(s)	1061, 1158
AfiI	CCNNNNNNNGG	4 cut(s)	103, 330, 769, 1123
AflIII	ACRYGT	2 cut(s)	558, 1570
AjnI	CCWGG	5 cut(s)	102, 329, 581, 1088, 1458
Alw21I	GWGCWC	2 cut(s)	1191, 1557
Alw26I	GTCTC	3 cut(s)	66, 1107, 2286
AlwI	GGATC	3 cut(s)	572, 1823, 2213
AlwNI	CAGNNNCTG	1 cut(s)	1337
AoxI	GGCC	7 cut(s)	6, 18, 118, 364, 760, 1213, 1845
ApeKI	GCWGC	5 cut(s)	49, 1241, 1805, 1819, 1898
ApoI	RAATTY	3 cut(s)	730, 1293, 2252
Asp700I	GAANNNNTTC	1 cut(s)	1400
AspLEI	GCGC	3 cut(s)	1013, 1680, 1682
AspS9I	GGNCC	6 cut(s)	333, 364, 585, 1214, 1845, 1914
AsuHPI	GGTGA	7 cut(s)	116, 424, 587, 823, 949, 2123, 2300
AvaII	GGWCC	3 cut(s)	333, 585, 1914
AxyI	CCTNAGG	1 cut(s)	221
BaeGI	GKGCMC	1 cut(s)	926
BanII	GRGCYC	1 cut(s)	1191
Bbv12I	GWGCWC	2 cut(s)	1191, 1557
BbvI	GCAGC	5 cut(s)	36, 1253, 1806, 1817, 1885
BccI	CCATC	6 cut(s)	355, 404, 1881, 1994, 2032, 2255
BceAI	ACGGC	1 cut(s)	351
BciT130I	CCWGG	5 cut(s)	104, 331, 583, 1090, 1460
BciVI	GTATCC	1 cut(s)	740
BclI	TGATCA	2 cut(s)	951, 1449
BcoDI	GTCTC	3 cut(s)	66, 1107, 2286
BfaI	CTAG	1 cut(s)	1793
BfmI	CTRYAG	2 cut(s)	130, 815
BfuI	GTATCC	1 cut(s)	740
BglI	GCCNNNNNGGC	1 cut(s)	1090
BglII	AGATCT	1 cut(s)	1789
BisI	GCNGC	5 cut(s)	50, 1242, 1806, 1820, 1899
BlpI	GCTNAGC	1 cut(s)	915
BlsI	GCNGC	5 cut(s)	51, 1243, 1807, 1821, 1900
Bme1390I	CCNGG	5 cut(s)	104, 331, 583, 1090, 1460
Bme18I	GGWCC	3 cut(s)	333, 585, 1914
BmgT120I	GGNCC	6 cut(s)	333, 364, 585, 1214, 1845, 1914
BmiI	GGNNCC	2 cut(s)	1216, 1846
BmrFI	CCNGG	5 cut(s)	104, 331, 583, 1090, 1460
BmrI	ACTGGG	1 cut(s)	421
BmsI	GCATC	7 cut(s)	181, 922, 1168, 1389, 1474, 1497, 2121
BmuI	ACTGGG	1 cut(s)	421
BpmI	CTGGAG	1 cut(s)	2169
Bpu10I	CCTNAGC	2 cut(s)	835, 1237
Bpu1102I	GCTNAGC	1 cut(s)	915
BpuEI	CTTGAG	1 cut(s)	887
BsaI	GGTCTC	1 cut(s)	1107
BsaJI	CCNNGG	3 cut(s)	102, 581, 1458
BsaXI	ACNNNNNCTCC	2 cut(s)	405, 435
Bsc4I	CCNNNNNNNGG	4 cut(s)	103, 330, 769, 1123
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	2 cut(s)	427, 1724
Bse21I	CCTNAGG	1 cut(s)	221
Bse3DI	GCAATG	6 cut(s)	312, 345, 668, 1146, 1351, 2160
BseBI	CCWGG	5 cut(s)	104, 331, 583, 1090, 1460
BseDI	CCNNGG	3 cut(s)	102, 581, 1458
BseGI	GGATG	3 cut(s)	415, 615, 1081
BseLI	CCNNNNNNNGG	4 cut(s)	103, 330, 769, 1123
BseMI	GCAATG	6 cut(s)	312, 345, 668, 1146, 1351, 2160
BseMII	CTCAG	6 cut(s)	76, 281, 704, 906, 1866, 2106
BseNI	ACTGG	2 cut(s)	427, 1724
BsePI	GCGCGC	1 cut(s)	1678
BseSI	GKGCMC	1 cut(s)	926
BseXI	GCAGC	5 cut(s)	36, 1253, 1806, 1817, 1885
BseYI	CCCAGC	1 cut(s)	919
BsgI	GTGCAG	2 cut(s)	416, 1884
Bsh1236I	CGCG	4 cut(s)	201, 590, 1013, 1680
BshFI	GGCC	7 cut(s)	8, 20, 120, 366, 762, 1215, 1847
BsiHKAI	GWGCWC	2 cut(s)	1191, 1557
BsiSI	CCGG	4 cut(s)	17, 84, 117, 177
BslI	CCNNNNNNNGG	4 cut(s)	103, 330, 769, 1123
BsmAI	GTCTC	3 cut(s)	66, 1107, 2286
BsmBI	CGTCTC	1 cut(s)	2286
BsmI	GAATGC	5 cut(s)	382, 643, 843, 1327, 1700
BsnI	GGCC	7 cut(s)	8, 20, 120, 366, 762, 1215, 1847
Bso31I	GGTCTC	1 cut(s)	1107
Bsp1286I	GDGCHC	3 cut(s)	926, 1191, 1557
Bsp1407I	TGTACA	1 cut(s)	1156
Bsp143I	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
Bsp1720I	GCTNAGC	1 cut(s)	915
BspANI	GGCC	7 cut(s)	8, 20, 120, 366, 762, 1215, 1847
BspCNI	CTCAG	6 cut(s)	75, 282, 703, 907, 1865, 2105
BspFNI	CGCG	4 cut(s)	201, 590, 1013, 1680
BspHI	TCATGA	2 cut(s)	156, 2208
BspLI	GGNNCC	2 cut(s)	1216, 1846
BspMAI	CTGCAG	1 cut(s)	134
BspPI	GGATC	3 cut(s)	572, 1823, 2213
BspQI	GCTCTTC	1 cut(s)	1196
BspTNI	GGTCTC	1 cut(s)	1107
BsrBI	CCGCTC	1 cut(s)	2322
BsrDI	GCAATG	6 cut(s)	312, 345, 668, 1146, 1351, 2160
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	1 cut(s)	1156
BsrI	ACTGG	2 cut(s)	427, 1724
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	3 cut(s)	102, 581, 1458
BssHII	GCGCGC	1 cut(s)	1678
BssMI	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
BssNAI	GTATAC	1 cut(s)	1568
Bst1107I	GTATAC	1 cut(s)	1568
Bst2UI	CCWGG	5 cut(s)	104, 331, 583, 1090, 1460
Bst4CI	ACNGT	7 cut(s)	163, 548, 1118, 1432, 1497, 1841, 2147
Bst6I	CTCTTC	1 cut(s)	1196
BstAPI	GCANNNNNTGC	2 cut(s)	461, 1802
BstAUI	TGTACA	1 cut(s)	1156
BstC8I	GCNNGC	8 cut(s)	54, 58, 841, 1086, 1553, 1657, 1680, 2324
BstF5I	GGATG	3 cut(s)	415, 615, 1081
BstFNI	CGCG	4 cut(s)	201, 590, 1013, 1680
BstHHI	GCGC	3 cut(s)	1013, 1680, 1682
BstKTI	GATC	8 cut(s)	580, 954, 1024, 1452, 1669, 1792, 1831, 2208
BstMAI	GTCTC	3 cut(s)	66, 1107, 2286
BstMBI	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 461, 470, 680, 1043, 1090, 1136, 1802, 1853
BstNI	CCWGG	5 cut(s)	104, 331, 583, 1090, 1460
BstNSI	RCATGY	4 cut(s)	459, 562, 1574, 1659
BstSCI	CCNGG	5 cut(s)	102, 329, 581, 1088, 1458
BstSFI	CTRYAG	2 cut(s)	130, 815
BstSLI	GKGCMC	1 cut(s)	926
BstUI	CGCG	4 cut(s)	201, 590, 1013, 1680
BstV1I	GCAGC	5 cut(s)	36, 1253, 1806, 1817, 1885
BstX2I	RGATCY	1 cut(s)	1789
BstXI	CCANNNNNNTGG	2 cut(s)	1315, 2269
BstYI	RGATCY	1 cut(s)	1789
BstZ17I	GTATAC	1 cut(s)	1568
Bsu36I	CCTNAGG	1 cut(s)	221
BsuI	GTATCC	1 cut(s)	740
BsuRI	GGCC	7 cut(s)	8, 20, 120, 366, 762, 1215, 1847
BtsCI	GGATG	3 cut(s)	415, 615, 1081
BtsI	GCAGTG	2 cut(s)	139, 2333
BtsIMutI	CAGTG	7 cut(s)	139, 287, 776, 1846, 2085, 2152, 2333
Cac8I	GCNNGC	8 cut(s)	54, 58, 841, 1086, 1553, 1657, 1680, 2324
CaiI	CAGNNNCTG	1 cut(s)	1337
CciI	TCATGA	2 cut(s)	156, 2208
CfoI	GCGC	3 cut(s)	1013, 1680, 1682
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	6 cut(s)	333, 364, 585, 1214, 1845, 1914
CseI	GACGC	1 cut(s)	2303
Csp6I	GTAC	2 cut(s)	1060, 1157
CspCI	CAANNNNNGTGG	2 cut(s)	327, 362
CviQI	GTAC	2 cut(s)	1060, 1157
DpnI	GATC	8 cut(s)	579, 953, 1023, 1451, 1668, 1791, 1830, 2207
DpnII	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
DraI	TTTAAA	1 cut(s)	555
DraIII	CACNNNGTG	1 cut(s)	202
Eam1104I	CTCTTC	1 cut(s)	1196
EarI	CTCTTC	1 cut(s)	1196
Ecl136II	GAGCTC	1 cut(s)	1189
Eco147I	AGGCCT	1 cut(s)	8
Eco24I	GRGCYC	1 cut(s)	1191
Eco31I	GGTCTC	1 cut(s)	1107
Eco47I	GGWCC	3 cut(s)	333, 585, 1914
Eco53kI	GAGCTC	1 cut(s)	1189
Eco57I	CTGAAG	1 cut(s)	785
Eco81I	CCTNAGG	1 cut(s)	221
EcoICRI	GAGCTC	1 cut(s)	1189
EcoO109I	RGGNCCY	2 cut(s)	1214, 1845
EcoRI	GAATTC	1 cut(s)	730
EcoRII	CCWGG	5 cut(s)	102, 329, 581, 1088, 1458
EcoT22I	ATGCAT	2 cut(s)	1657, 1661
EcoT38I	GRGCYC	1 cut(s)	1191
Esp3I	CGTCTC	1 cut(s)	2286
FalI	AAGNNNNNCTT	6 cut(s)	59, 91, 818, 850, 1000, 1032
FauI	CCCGC	1 cut(s)	2315
FauNDI	CATATG	1 cut(s)	657
FbaI	TGATCA	2 cut(s)	951, 1449
FblI	GTMKAC	1 cut(s)	1567
Fnu4HI	GCNGC	5 cut(s)	50, 1242, 1806, 1820, 1899
FokI	GGATG	3 cut(s)	422, 622, 1088
FriOI	GRGCYC	1 cut(s)	1191
Fsp4HI	GCNGC	5 cut(s)	50, 1242, 1806, 1820, 1899
FspBI	CTAG	1 cut(s)	1793
GlaI	GCGC	3 cut(s)	1012, 1679, 1681
GluI	GCNGC	5 cut(s)	50, 1242, 1806, 1820, 1899
GsaI	CCCAGC	1 cut(s)	923
GsuI	CTGGAG	1 cut(s)	2169
HaeIII	GGCC	7 cut(s)	8, 20, 120, 366, 762, 1215, 1847
HapII	CCGG	4 cut(s)	17, 84, 117, 177
HgaI	GACGC	1 cut(s)	2303
HhaI	GCGC	3 cut(s)	1013, 1680, 1682
Hin6I	GCGC	3 cut(s)	1011, 1678, 1680
HinP1I	GCGC	3 cut(s)	1011, 1678, 1680
HindIII	AAGCTT	2 cut(s)	1067, 2264
HinfI	GANTC	6 cut(s)	320, 806, 1103, 1960, 1966, 2090
HpaII	CCGG	4 cut(s)	17, 84, 117, 177
HphI	GGTGA	7 cut(s)	116, 424, 587, 823, 949, 2123, 2300
Hpy166II	GTNNAC	7 cut(s)	166, 432, 831, 1568, 1624, 1754, 2008
Hpy188I	TCNGA	7 cut(s)	31, 65, 190, 360, 387, 1021, 1965
Hpy188III	TCNNGA	8 cut(s)	157, 803, 1694, 1832, 2081, 2186, 2209, 2339
Hpy8I	GTNNAC	7 cut(s)	166, 432, 831, 1568, 1624, 1754, 2008
Hpy99I	CGWCG	2 cut(s)	212, 2200
HpyAV	CCTTC	5 cut(s)	131, 267, 796, 1905, 2116
HpyCH4III	ACNGT	7 cut(s)	163, 548, 1118, 1432, 1497, 1841, 2147
HpyCH4IV	ACGT	2 cut(s)	1620, 2198
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 461, 470, 680, 1043, 1090, 1136, 1802, 1853
HpySE526I	ACGT	2 cut(s)	1620, 2198
HspAI	GCGC	3 cut(s)	1011, 1678, 1680
Ksp22I	TGATCA	2 cut(s)	951, 1449
Kzo9I	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
LguI	GCTCTTC	1 cut(s)	1196
LmnI	GCTCC	2 cut(s)	1186, 1596
Lsp1109I	GCAGC	5 cut(s)	36, 1253, 1806, 1817, 1885
LweI	GCATC	7 cut(s)	181, 922, 1168, 1389, 1474, 1497, 2121
MaeI	CTAG	1 cut(s)	1793
MaeII	ACGT	2 cut(s)	1620, 2198
MaeIII	GTNAC	5 cut(s)	350, 368, 421, 1472, 1512
MalI	GATC	8 cut(s)	579, 953, 1023, 1451, 1668, 1791, 1830, 2207
MbiI	CCGCTC	1 cut(s)	2322
MboI	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
MfeI	CAATTG	1 cut(s)	984
MflI	RGATCY	1 cut(s)	1789
MhlI	GDGCHC	3 cut(s)	926, 1191, 1557
MluCI	AATT	8 cut(s)	730, 984, 1293, 1353, 1405, 1606, 1705, 2252
MlyI	GAGTC	3 cut(s)	314, 1097, 2084
MmeI	TCCRAC	2 cut(s)	855, 1937
Mph1103I	ATGCAT	2 cut(s)	1657, 1661
MroXI	GAANNNNTTC	1 cut(s)	1400
MslI	CAYNNNNRTG	6 cut(s)	235, 386, 1199, 1302, 1782, 2145
MspA1I	CMGCKG	2 cut(s)	49, 1808
MspI	CCGG	4 cut(s)	17, 84, 117, 177
MspR9I	CCNGG	5 cut(s)	104, 331, 583, 1090, 1460
MunI	CAATTG	1 cut(s)	984
Mva1269I	GAATGC	5 cut(s)	382, 643, 843, 1327, 1700
MvaI	CCWGG	5 cut(s)	104, 331, 583, 1090, 1460
MvnI	CGCG	4 cut(s)	201, 590, 1013, 1680
MwoI	GCNNNNNNNGC	9 cut(s)	191, 461, 470, 680, 1043, 1090, 1136, 1802, 1853
NdeI	CATATG	1 cut(s)	657
NdeII	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
NlaIV	GGNNCC	2 cut(s)	1216, 1846
NmuCI	GTSAC	3 cut(s)	421, 1472, 1512
NsiI	ATGCAT	2 cut(s)	1657, 1661
NspI	RCATGY	4 cut(s)	459, 562, 1574, 1659
PaeI	GCATGC	1 cut(s)	1659
PagI	TCATGA	2 cut(s)	156, 2208
PauI	GCGCGC	1 cut(s)	1678
PceI	AGGCCT	1 cut(s)	8
PciI	ACATGT	2 cut(s)	558, 1570
PciSI	GCTCTTC	1 cut(s)	1196
PctI	GAATGC	5 cut(s)	382, 643, 843, 1327, 1700
PdmI	GAANNNNTTC	1 cut(s)	1400
PfeI	GAWTC	3 cut(s)	806, 1960, 1966
PfoI	TCCNGGA	1 cut(s)	329
PkrI	GCNGC	5 cut(s)	51, 1243, 1807, 1821, 1900
PleI	GAGTC	3 cut(s)	314, 1097, 2084
PpsI	GAGTC	3 cut(s)	314, 1097, 2084
PscI	ACATGT	2 cut(s)	558, 1570
Psp124BI	GAGCTC	1 cut(s)	1191
Psp6I	CCWGG	5 cut(s)	102, 329, 581, 1088, 1458
PspFI	CCCAGC	1 cut(s)	919
PspGI	CCWGG	5 cut(s)	102, 329, 581, 1088, 1458
PspN4I	GGNNCC	2 cut(s)	1216, 1846
PspPI	GGNCC	6 cut(s)	333, 364, 585, 1214, 1845, 1914
PstI	CTGCAG	1 cut(s)	134
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	1 cut(s)	1789
PteI	GCGCGC	1 cut(s)	1678
PvuII	CAGCTG	2 cut(s)	49, 1808
RsaI	GTAC	2 cut(s)	1061, 1158
RsaNI	GTAC	2 cut(s)	1060, 1157
RseI	CAYNNNNRTG	6 cut(s)	235, 386, 1199, 1302, 1782, 2145
SacI	GAGCTC	1 cut(s)	1191
SapI	GCTCTTC	1 cut(s)	1196
SatI	GCNGC	5 cut(s)	50, 1242, 1806, 1820, 1899
Sau3AI	GATC	8 cut(s)	577, 951, 1021, 1449, 1666, 1789, 1828, 2205
Sau96I	GGNCC	6 cut(s)	333, 364, 585, 1214, 1845, 1914
SchI	GAGTC	3 cut(s)	314, 1097, 2084
ScrFI	CCNGG	5 cut(s)	104, 331, 583, 1090, 1460
SduI	GDGCHC	3 cut(s)	926, 1191, 1557
SfaNI	GCATC	7 cut(s)	181, 922, 1168, 1389, 1474, 1497, 2121
SfcI	CTRYAG	2 cut(s)	130, 815
SinI	GGWCC	3 cut(s)	333, 585, 1914
SmiMI	CAYNNNNRTG	6 cut(s)	235, 386, 1199, 1302, 1782, 2145
SmlI	CTYRAG	1 cut(s)	866
SmoI	CTYRAG	1 cut(s)	866
SphI	GCATGC	1 cut(s)	1659
Sse9I	AATT	8 cut(s)	730, 984, 1293, 1353, 1405, 1606, 1705, 2252
SseBI	AGGCCT	1 cut(s)	8
SspMI	CTAG	1 cut(s)	1793
SstI	GAGCTC	1 cut(s)	1191
StuI	AGGCCT	1 cut(s)	8
StyD4I	CCNGG	5 cut(s)	102, 329, 581, 1088, 1458
TaaI	ACNGT	7 cut(s)	163, 548, 1118, 1432, 1497, 1841, 2147
TaiI	ACGT	2 cut(s)	1623, 2201
TaqI	TCGA	5 cut(s)	207, 603, 629, 1262, 1632
TasI	AATT	8 cut(s)	730, 984, 1293, 1353, 1405, 1606, 1705, 2252
TatI	WGTACW	1 cut(s)	1156
TfiI	GAWTC	3 cut(s)	806, 1960, 1966
TscAI	CASTG	7 cut(s)	139, 294, 783, 1846, 2092, 2152, 2333
TseFI	GTSAC	3 cut(s)	421, 1472, 1512
TseI	GCWGC	5 cut(s)	49, 1241, 1805, 1819, 1898
Tsp45I	GTSAC	3 cut(s)	421, 1472, 1512
TspDTI	ATGAA	9 cut(s)	161, 485, 719, 1183, 1286, 1766, 1934, 1970, 2225
TspGWI	ACGGA	1 cut(s)	313
TspRI	CASTG	7 cut(s)	139, 294, 783, 1846, 2092, 2152, 2333
VpaK11BI	GGWCC	3 cut(s)	333, 585, 1914
XapI	RAATTY	3 cut(s)	730, 1293, 2252
XceI	RCATGY	4 cut(s)	459, 562, 1574, 1659
XmiI	GTMKAC	1 cut(s)	1567
XmnI	GAANNNNTTC	1 cut(s)	1400
XspI	CTAG	1 cut(s)	1793
Zsp2I	ATGCAT	2 cut(s)	1657, 1661

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.