Rorug02G0201400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008593
Physical Location & Seq
Reverse (-)
18832065 .. 18837868
5804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0201400.1

Sequence Viewer

Length: 1347 bp
ATGAAATTTGATCTATATGATGAGGTATCACGTACCATTACAGTGGATATGAAGGGTCGAGGAGATTTTAGAACAGTTCAAGAAGCCATTGATTCCATACCCTCAAATAACTCTTGGTGGATTCGAATCCAACTTAACAATGGTCTCTACTTTGAAAAAGTGCTGATTCCTGAAGACAAACCATACATTATTCTTGAAGGTCACCCTAAATATCTCACTGCAATAGAATTTGGAGACTTTGGCAGTGTTGCAGAGAGTCCTACATTTACGTTACTTGCAGATAATTTCGTAGCTCGGAATATACTATTCAAGGTTAAAGGATCTAGTTGGATCCTAATAACCGGAGAACTACTTGGGCAGTACGTTGCGGCTAAAATTGTTGGAGACAAAGCAAGTTTTTATCATTGTGGTTTCATTAGCTTGCAAGATACACTGTGGGATGCCAAAGGTCGTCACTATTTCTATGATTGCGATGTTCGTGGTGCTATAGACTTTATTTGGGGCAATGGCCAGTCAATTTATGAGAAATGTCAGATAATGTCTTTGAGCAAATTAATTGGCACTACTAGCTATATTACAGCACAAGGTCGTGAAGGTCCAGACGAAACTACTGGTTTTGTATTCAAAGATTGTTATGTATGTGGATCGGGCACTGCATTTCTTGGGAGAGCATACAGAGACTACTCAAGAGTCTTGTTTTCAGGGACGTATATGGAAAATGTTATTACCCCGGAGGGTTGGTCCCTTTGGACACCGCCACAAATTCAACATGAGGATGTGATCACATTCTCAGAGGCGAATTGCAGAGGACCTGGAGCAGATATGTCCCGTCGTATTTGGTGGGAGAAGAAGCTATCAGATGAGGAAGGCCAAAGTCCTAGCCCAAAACTCAAAGCCCAACTAGGGCAGCCACCACAACAAGTCCACCCAAGGCCCATTCAGTTGATCCGGTCCAGTCCACCAACACCATCTGCTCTCCCGTCGTTCACAACAGTCAAAGCGAAGCTCCCTCAGCCGCACGCCGCCATGACCGGCACCGCCTCGATCCCCTCCGCAGCCGTCCCACCGCCTGCGACCCACACCCCCACAGGCCCCGACGTTGCAACCCACGCACAGAGAAAACCAAACCGATCCAGATCGTCACAACACCACCGCAGAGAAAAACCCAACCCCGATCCCGGTCTCCTGCCACACACCGCCATCGAGTCTGCTCCTTGGCGCCGTCGATCAAGAAAAACAGTCATCAGAGCTAGATCTCCGCTCCCATTTGAACGACCACCACCACGCACCATCCCAGCCAAACCCTCCGACAAGCCCCGAGCAAACTCATCTGATGGAACCCCTTGA

Protein Analysis

448

Amino Acids

49.98

Weight (kDa)

9.13

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 13 - 283 3.7e-54 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1034, 1218
AccBSI CCGCTC 1 cut(s) 1261
AclWI GGATC 8 cut(s) 325, 328, 338, 652, 940, 1039, 1125, 1169
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 3 cut(s) 5, 227, 762
AcuI CTGAAG 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 1219
AfaI GTAC 2 cut(s) 34, 362
AfiI CCNNNNNNNGG 2 cut(s) 903, 1178
AgsI TTSAA 7 cut(s) 80, 155, 197, 310, 625, 767, 1271
AjnI CCWGG 1 cut(s) 811
AluBI AGCT 6 cut(s) 293, 420, 570, 853, 1006, 1250
AluI AGCT 6 cut(s) 293, 420, 570, 853, 1006, 1250
Alw26I GTCTC 5 cut(s) 149, 228, 378, 672, 1187
AlwI GGATC 8 cut(s) 325, 328, 338, 652, 940, 1039, 1125, 1169
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 4 cut(s) 508, 868, 932, 1090
ApeKI GCWGC 2 cut(s) 907, 1055
ApoI RAATTY 3 cut(s) 5, 227, 762
AseI ATTAAT 1 cut(s) 554
AspLEI GCGC 1 cut(s) 1221
AspS9I GGNCC 6 cut(s) 596, 741, 809, 933, 951, 1091
AsuC2I CCSGG 2 cut(s) 731, 1179
AsuHPI GGTGA 1 cut(s) 194
AsuII TTCGAA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 4 cut(s) 596, 741, 809, 951
BaeGI GKGCMC 1 cut(s) 653
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 330
BanI GGYRCC 2 cut(s) 1034, 1218
BbsI GAAGAC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 2 cut(s) 919, 1067
BccI CCATC 4 cut(s) 976, 1208, 1298, 1328
BceAI ACGGC 2 cut(s) 1043, 1206
BciT130I CCWGG 1 cut(s) 813
BclI TGATCA 1 cut(s) 780
BcnI CCSGG 2 cut(s) 731, 1179
BcoDI GTCTC 5 cut(s) 149, 228, 378, 672, 1187
BfaI CTAG 5 cut(s) 324, 567, 879, 902, 1251
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 1222
BglII AGATCT 1 cut(s) 1253
BisI GCNGC 5 cut(s) 369, 908, 1016, 1023, 1056
BlsI GCNGC 5 cut(s) 370, 909, 1017, 1024, 1057
Bme1390I CCNGG 3 cut(s) 731, 813, 1179
Bme18I GGWCC 4 cut(s) 596, 741, 809, 951
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 6 cut(s) 596, 741, 809, 933, 951, 1091
BmiI GGNNCC 6 cut(s) 332, 743, 1036, 1093, 1220, 1339
BmrFI CCNGG 3 cut(s) 731, 813, 1179
BmsI GCATC 1 cut(s) 430
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 834
Bpu10I CCTNAGC 1 cut(s) 1011
Bpu14I TTCGAA 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 670
BpuMI CCSGG 2 cut(s) 731, 1179
BsaAI YACGTR 1 cut(s) 32
BsaBI GATNNNNATC 2 cut(s) 125, 1135
BsaHI GRCGYC 1 cut(s) 1219
BsaI GGTCTC 2 cut(s) 149, 1187
BsaJI CCNNGG 3 cut(s) 729, 929, 1214
BsaWI WCCGGW 2 cut(s) 341, 948
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 2 cut(s) 903, 1178
Bse118I RCCGGY 1 cut(s) 1031
Bse1I ACTGG 3 cut(s) 511, 616, 954
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 2 cut(s) 125, 1135
BseBI CCWGG 1 cut(s) 813
BseDI CCNNGG 3 cut(s) 729, 929, 1214
BseGI GGATG 3 cut(s) 445, 781, 1290
BseJI GATNNNNATC 2 cut(s) 125, 1135
BseLI CCNNNNNNNGG 2 cut(s) 903, 1178
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 2 cut(s) 804, 1025
BseNI ACTGG 3 cut(s) 511, 616, 954
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 2 cut(s) 919, 1067
BseYI CCCAGC 1 cut(s) 1294
BshFI GGCC 4 cut(s) 510, 870, 934, 1092
BshNI GGYRCC 2 cut(s) 1034, 1218
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 5 cut(s) 342, 731, 949, 1032, 1179
BslFI GGGAC 4 cut(s) 718, 727, 811, 1046
BslI CCNNNNNNNGG 2 cut(s) 903, 1178
BsmAI GTCTC 5 cut(s) 149, 228, 378, 672, 1187
BsmFI GGGAC 4 cut(s) 718, 727, 811, 1046
BsnI GGCC 4 cut(s) 510, 870, 934, 1092
Bso31I GGTCTC 2 cut(s) 149, 1187
BsoBI CYCGRG 1 cut(s) 1317
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 653
BspANI GGCC 4 cut(s) 510, 870, 934, 1092
BspCNI CTCAG 2 cut(s) 803, 1024
BspLI GGNNCC 6 cut(s) 332, 743, 1036, 1093, 1220, 1339
BspPI GGATC 8 cut(s) 325, 328, 338, 652, 940, 1039, 1125, 1169
BspT104I TTCGAA 1 cut(s) 124
BspT107I GGYRCC 2 cut(s) 1034, 1218
BspTNI GGTCTC 2 cut(s) 149, 1187
BsrBI CCGCTC 1 cut(s) 1261
BsrDI GCAATG 1 cut(s) 511
BsrFI RCCGGY 1 cut(s) 1031
BsrI ACTGG 3 cut(s) 511, 616, 954
BssAI RCCGGY 1 cut(s) 1031
BssECI CCNNGG 3 cut(s) 729, 929, 1214
BssNI GRCGYC 1 cut(s) 1219
BssT1I CCWWGG 2 cut(s) 929, 1214
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 5 cut(s) 43, 76, 435, 994, 1240
BstACI GRCGYC 1 cut(s) 1219
BstBAI YACGTR 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 3 cut(s) 422, 1020, 1071
BstDEI CTNAG 2 cut(s) 790, 1011
BstEII GGTNACC 1 cut(s) 200
BstF5I GGATG 3 cut(s) 445, 781, 1290
BstH2I RGCGCY 1 cut(s) 1222
BstHHI GCGC 1 cut(s) 1221
BstMAI GTCTC 5 cut(s) 149, 228, 378, 672, 1187
BstMWI GCNNNNNNNGC 3 cut(s) 567, 1012, 1109
BstNI CCWGG 1 cut(s) 813
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 3 cut(s) 729, 811, 1177
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 653
BstV1I GCAGC 2 cut(s) 919, 1067
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 3 cut(s) 320, 330, 1253
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 3 cut(s) 320, 330, 1253
BsuRI GGCC 4 cut(s) 510, 870, 934, 1092
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 3 cut(s) 445, 781, 1290
BtsI GCAGTG 3 cut(s) 216, 250, 651
BtsIMutI CAGTG 5 cut(s) 48, 216, 250, 431, 651
Cac8I GCNNGC 3 cut(s) 422, 1020, 1071
CfoI GCGC 1 cut(s) 1221
Cfr10I RCCGGY 1 cut(s) 1031
Cfr13I GGNCC 6 cut(s) 596, 741, 809, 933, 951, 1091
Csp6I GTAC 2 cut(s) 33, 361
CviAII CATG 2 cut(s) 770, 1027
CviQI GTAC 2 cut(s) 33, 361
DdeI CTNAG 2 cut(s) 790, 1011
DinI GGCGCC 1 cut(s) 1220
EaeI YGGCCR 1 cut(s) 508
Eco130I CCWWGG 2 cut(s) 929, 1214
Eco31I GGTCTC 2 cut(s) 149, 1187
Eco47I GGWCC 4 cut(s) 596, 741, 809, 951
Eco57I CTGAAG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 1317
Eco91I GGTNACC 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 809, 1091
EcoO65I GGTNACC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 811
EcoT14I CCWWGG 2 cut(s) 929, 1214
EgeI GGCGCC 1 cut(s) 1220
EheI GGCGCC 1 cut(s) 1220
ErhI CCWWGG 2 cut(s) 929, 1214
FaeI CATG 2 cut(s) 773, 1030
FaqI GGGAC 4 cut(s) 718, 727, 811, 1046
FatI CATG 2 cut(s) 769, 1026
FbaI TGATCA 1 cut(s) 780
Fnu4HI GCNGC 5 cut(s) 369, 908, 1016, 1023, 1056
FokI GGATG 3 cut(s) 452, 788, 1277
Fsp4HI GCNGC 5 cut(s) 369, 908, 1016, 1023, 1056
FspBI CTAG 5 cut(s) 324, 567, 879, 902, 1251
GlaI GCGC 1 cut(s) 1220
GluI GCNGC 5 cut(s) 369, 908, 1016, 1023, 1056
GsaI CCCAGC 1 cut(s) 1298
GsuI CTGGAG 1 cut(s) 834
HaeII RGCGCY 1 cut(s) 1222
HaeIII GGCC 4 cut(s) 510, 870, 934, 1092
HapII CCGG 5 cut(s) 342, 731, 949, 1032, 1179
HhaI GCGC 1 cut(s) 1221
Hin1I GRCGYC 1 cut(s) 1219
Hin1II CATG 2 cut(s) 773, 1030
Hin6I GCGC 1 cut(s) 1219
HinP1I GCGC 1 cut(s) 1219
HinfI GANTC 7 cut(s) 92, 121, 126, 166, 256, 690, 1205
HpaII CCGG 5 cut(s) 342, 731, 949, 1032, 1179
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 3 cut(s) 925, 959, 987
Hpy188I TCNGA 7 cut(s) 297, 534, 793, 859, 1247, 1309, 1333
Hpy188III TCNNGA 8 cut(s) 80, 170, 194, 590, 599, 687, 1134, 1230
Hpy8I GTNNAC 3 cut(s) 925, 959, 987
Hpy99I CGWCG 4 cut(s) 834, 985, 1100, 1227
HpyAV CCTTC 4 cut(s) 46, 191, 587, 860
HpyCH4III ACNGT 5 cut(s) 43, 76, 435, 994, 1240
HpyCH4IV ACGT 5 cut(s) 31, 269, 363, 707, 1098
HpyCH4V TGCA 7 cut(s) 221, 251, 278, 424, 656, 804, 1103
HpyF10VI GCNNNNNNNGC 3 cut(s) 567, 1012, 1109
HpyF3I CTNAG 2 cut(s) 790, 1011
HpySE526I ACGT 5 cut(s) 31, 269, 363, 707, 1098
Hsp92I GRCGYC 1 cut(s) 1219
Hsp92II CATG 2 cut(s) 773, 1030
HspAI GCGC 1 cut(s) 1219
KasI GGCGCC 1 cut(s) 1218
Ksp22I TGATCA 1 cut(s) 780
LmnI GCTCC 4 cut(s) 815, 1011, 1216, 1266
Lsp1109I GCAGC 2 cut(s) 919, 1067
LweI GCATC 1 cut(s) 430
MaeI CTAG 5 cut(s) 324, 567, 879, 902, 1251
MaeII ACGT 5 cut(s) 31, 269, 363, 707, 1098
MaeIII GTNAC 4 cut(s) 200, 270, 452, 1140
MbiI CCGCTC 1 cut(s) 1261
MboII GAAGA 2 cut(s) 185, 859
MflI RGATCY 3 cut(s) 320, 330, 1253
MhlI GDGCHC 1 cut(s) 653
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 9 cut(s) 5, 227, 283, 375, 516, 551, 555, 762, 799
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 1219
MlyI GAGTC 3 cut(s) 265, 699, 1214
MmeI TCCRAC 4 cut(s) 154, 308, 361, 1332
Mox20I TGGCCA 1 cut(s) 510
MscI TGGCCA 1 cut(s) 510
MseI TTAA 3 cut(s) 135, 315, 554
MslI CAYNNNNRTG 2 cut(s) 41, 774
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 5 cut(s) 342, 731, 949, 1032, 1179
MspR9I CCNGG 3 cut(s) 731, 813, 1179
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 3 cut(s) 567, 1012, 1109
NarI GGCGCC 1 cut(s) 1219
NciI CCSGG 2 cut(s) 731, 1179
NlaIII CATG 2 cut(s) 773, 1030
NlaIV GGNNCC 6 cut(s) 332, 743, 1036, 1093, 1220, 1339
NmuCI GTSAC 3 cut(s) 200, 452, 1140
NspV TTCGAA 1 cut(s) 124
PfeI GAWTC 4 cut(s) 92, 121, 126, 166
PkrI GCNGC 5 cut(s) 370, 909, 1017, 1024, 1057
PleI GAGTC 3 cut(s) 264, 698, 1213
PluTI GGCGCC 1 cut(s) 1222
PpsI GAGTC 3 cut(s) 264, 698, 1213
Ppu21I YACGTR 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 809
PshBI ATTAAT 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 811
PspEI GGTNACC 1 cut(s) 200
PspFI CCCAGC 1 cut(s) 1294
PspGI CCWGG 1 cut(s) 811
PspN4I GGNNCC 6 cut(s) 332, 743, 1036, 1093, 1220, 1339
PspPI GGNCC 6 cut(s) 596, 741, 809, 933, 951, 1091
PspPPI RGGWCCY 1 cut(s) 809
PsuI RGATCY 3 cut(s) 320, 330, 1253
RsaI GTAC 2 cut(s) 34, 362
RsaNI GTAC 2 cut(s) 33, 361
RseI CAYNNNNRTG 2 cut(s) 41, 774
SaqAI TTAA 3 cut(s) 135, 315, 554
SatI GCNGC 5 cut(s) 369, 908, 1016, 1023, 1056
Sau96I GGNCC 6 cut(s) 596, 741, 809, 933, 951, 1091
SchI GAGTC 3 cut(s) 265, 699, 1214
ScrFI CCNGG 3 cut(s) 731, 813, 1179
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 486
SfoI GGCGCC 1 cut(s) 1220
SfuI TTCGAA 1 cut(s) 124
SinI GGWCC 4 cut(s) 596, 741, 809, 951
SmiMI CAYNNNNRTG 2 cut(s) 41, 774
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
Sse9I AATT 9 cut(s) 5, 227, 283, 375, 516, 551, 555, 762, 799
SspDI GGCGCC 1 cut(s) 1218
SspMI CTAG 5 cut(s) 324, 567, 879, 902, 1251
StyD4I CCNGG 3 cut(s) 729, 811, 1177
StyI CCWWGG 2 cut(s) 929, 1214
TaaI ACNGT 5 cut(s) 43, 76, 435, 994, 1240
TaiI ACGT 5 cut(s) 34, 272, 366, 710, 1101
TaqI TCGA 5 cut(s) 58, 124, 1043, 1203, 1225
TasI AATT 9 cut(s) 5, 227, 283, 375, 516, 551, 555, 762, 799
TauI GCSGC 3 cut(s) 371, 1018, 1025
TfiI GAWTC 4 cut(s) 92, 121, 126, 166
Tru1I TTAA 3 cut(s) 135, 315, 554
Tru9I TTAA 3 cut(s) 135, 315, 554
TscAI CASTG 5 cut(s) 48, 223, 250, 438, 658
TseFI GTSAC 3 cut(s) 200, 452, 1140
TseI GCWGC 2 cut(s) 907, 1055
Tsp45I GTSAC 3 cut(s) 200, 452, 1140
TspDTI ATGAA 3 cut(s) 17, 65, 403
TspRI CASTG 5 cut(s) 48, 223, 250, 438, 658
VpaK11BI GGWCC 4 cut(s) 596, 741, 809, 951
VspI ATTAAT 1 cut(s) 554
XapI RAATTY 3 cut(s) 5, 227, 762
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XspI CTAG 5 cut(s) 324, 567, 879, 902, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.