RchiOBHm_Chr2g0115941

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008593
Physical Location & Seq
Reverse (-)
28131108 .. 28136610
5503 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48905

Sequence Viewer

Length: 2352 bp
ATGGAGGCCCCAAACCCTCTGACCACTTCGTCGGATTTCTACGCCTATCACCTCCAAGCATGCCTAAACAAGAGACACCCATTTGCCGGAAAATCCATCCATGCCCAGATCATCAAGTCAGGGCTTCACCTAGGTGTGTTCTTGATGAACAATCTCATGAACTATTACATCAAAACTGGGTCTTATGTCGATGCACGCAACGTGTTCGATGAAATGCCTGTGAGAAACAAGTTTTCATGGAACACGATTCTCTCTGGTTACACAAAGCAGGGAAGGCTCGATGCAGCTTGGGGTGTTTTTAATGAAATGCCTGAACGCGACTCGGTTTCGTGGACTGCGATGATTGTGGGTTATAATCAGATGGGGAGATATGGTAATGCGATTCGGATGTTCTTGGACATGGTTTCGGATGGAGTTCCTGTGACCCAGTTCACTGTTACTAACATTCTTTCGGCTTGCGCTTCGATTAAGGGTTTGGATATTGGTAGGAAGGTGCATTCCTTTGTTGTTAAGCTCGCAGTTGGCAGTTATGTTTCGGTTGGGAACTGCCTTTTAAATATGTATGTGAAATGTGGTGATCCGGGGACTGCACAGATTGTTTTTGATAGGATGAGATTGAAAAATGTATCGAGCTGGAATCATATGATTATGTTACATATGCAATGTGGTCGTGCAGACCTTGCTCTTGCACAGTTTAATCAAATGACGGAGCGCGACATTGTTTCTTGGAATTCAATTATTGCTGGATACAACCAGCATGGGCTCGAAATGGAAGCAGTGGCTACCTTCTTAAGTATGGTGAAGGATTCTTTTCTAAAGCCTGATAAGTTCACCTTGGCAAGCATCTTGTCAGCTTGTGCTAACCTTGAGAAGTTGGAACTTGGAAAACAAATACATGTTCTTATTATAAGAGCTGGATTGGACACATCCGAAGCAGTGGTTAATGCTTTGATTTCAATGTATGCCAAGTCTGGTGGGTTGGAAATTGCTGAAAAGGTTTTAGAGCAATGTGGGACTTCAGATCTCAATCTTATAGCTTTTACTTCCTTATTGGATGGATACATTAAGGTTGGGGATGTTAACCCAGCGAGACAGATATTTGACTCATTGAGAGAATGTGATGTGGTAGCATGGACAGCTATGATTGTCGGTTATGTGCAGAATGGGTTGAATAATGATGCTTTGGAGCTCTTTAGGTCAATGATAGAAGGAGGCCCCAGGCCAAACAACTACACCTTAGCAGCTATGTTAAGTGTCAGTTCAAGCTTGGCTTCTTTGGATTATGGAAAACAAATTCATGCAAGTGCTATACGAACAGGAGAAGTGTCTTCGGTTTCTGTGGGCAATGCTCTAATTACCATGTATTCCAAAGCTGGAAGCATCAGTGGTGCAAAGCAAGTTTTCAATCTGATTGACCGGAATAGAGATACTGTTTCTTGGAGTTCCATGATTATAGCCCTGGCTCAACATGGTCTTGGGGAAGAATCCATTGAACTATTTGAGAAGATGCTGTCACTTGGTATTAGGCCCGACCATGTAACTTATGTTGGGGTGCTCTCTGCTTGTACACATGTGGGGTTGGTCGACCAGGGCAGGAGCTACTATAATTTGATGAGGAGTGTACACAAGATTGAACCCACCCATAGCCATTATGCATGTATGGTTGATCTGCTTGGGCGTGCTGGGTTACTTCAAGAAGCATACGACGTGATACAAAATATGCCAATTGAACCTGATGTAATAGCTTGGGGTTCACTTTTATCTGCTTGTAAGCTTCATAAAAATGTTGATTTGGCTAAAGTTGCAGCAGACAAATTGCTCCTTATTGAACCAGACAACAGTGGGGCCTATTCAGCCCTTGCTAATTTGTATTCAGCTTGTGGAAGGTGGGTAGATGCTGCAGAAGTAAGAAAGCTAATGAAGAATAGGAGAGTGAAGAAAGGCCAAGGATTTAGTTGGCTTGAGTATCAGAACAAAGTCCATGTGTTTGGGGTTGATGATGGAGTTCACCCACAGAGAGATGGAATCTACAAGATGATGGAGAAAATCTGGAAAGAGATTAAAAATATCGGTTTTGTCCCTGATACTGAGTCAGTATTGCACGACGTTGCAGAAGAATTGAAGGAGAAGATGCTTATGCATCACAGTGAGAAACTTGCAATTGCATTTGGGCTAATAAATACTCCAGAGAACAGTACGCTGAGGATTATGAAGAACCTCAGAGTTTGTAATGATTGTCACTCTGCCATTAAATTTATATCCAAGCTTGTGGGAAGAGAAATCATTCTGAGAGACGCTACTCGTTTTCACCATTTTAAGAATGGGACTTGTTCTTGTCGGGACTATTGGTAG

Protein Analysis

783

Amino Acids

87.01

Weight (kDa)

7.83

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 106 8.8e-08 PPR repeat family
PPR PF01535 78 - 106 1.1e-06 PPR repeat
PPR_2 PF13041 107 - 154 9e-08 PPR repeat family
PPR_2 PF13041 238 - 288 5.7e-06 PPR repeat family
PPR_2 PF13041 374 - 417 3.7e-09 PPR repeat family
PPR PF01535 376 - 405 4.9e-06 PPR repeat
PPR_2 PF13041 475 - 522 8.4e-09 PPR repeat family
PPR PF01535 478 - 508 3.2e-07 PPR repeat
E_motif PF20431 593 - 655 7.1e-16 E motif
DYW_deaminase PF14432 691 - 783 3e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 354, 908
AasI GACNNNNNNGTC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 1584
AccII CGCG 2 cut(s) 318, 714
AclWI GGATC 1 cut(s) 572
AcsI RAATTY 3 cut(s) 730, 1293, 2252
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 3 cut(s) 1567, 1623, 2197
AfiI CCNNNNNNNGG 1 cut(s) 86
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 3 cut(s) 201, 895, 1570
AjiI CACGTC 1 cut(s) 1708
AjnI CCWGG 3 cut(s) 1217, 1458, 1587
AleI CACNNNNGTG 1 cut(s) 132
Alw21I GWGCWC 2 cut(s) 1191, 1557
Alw26I GTCTC 3 cut(s) 67, 1084, 2286
AlwI GGATC 1 cut(s) 572
AoxI GGCC 6 cut(s) 6, 1213, 1220, 1526, 1845, 1942
ApeKI GCWGC 4 cut(s) 284, 1241, 1805, 1898
ApoI RAATTY 3 cut(s) 730, 1293, 2252
Asp700I GAANNNNTTC 1 cut(s) 2283
AspA2I CCTAGG 1 cut(s) 130
AspLEI GCGC 2 cut(s) 461, 714
AspS9I GGNCC 4 cut(s) 7, 1214, 1527, 1845
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 7 cut(s) 41, 119, 587, 811, 823, 2000, 2300
AvrII CCTAGG 1 cut(s) 130
BanII GRGCYC 2 cut(s) 765, 1191
BbsI GAAGAC 1 cut(s) 1320
Bbv12I GWGCWC 2 cut(s) 1191, 1557
BbvCI CCTCAGC 1 cut(s) 2201
BbvI GCAGC 4 cut(s) 296, 1253, 1817, 1885
BccI CCATC 7 cut(s) 104, 355, 404, 1049, 1994, 2015, 2032
BcgI CGANNNNNNTGC 4 cut(s) 187, 221, 650, 684
BciT130I CCWGG 3 cut(s) 1219, 1460, 1589
BciVI GTATCC 2 cut(s) 740, 1052
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 3 cut(s) 67, 1084, 2286
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 1899
BfrI CTTAAG 1 cut(s) 790
BfuI GTATCC 2 cut(s) 740, 1052
BglII AGATCT 1 cut(s) 1021
BisI GCNGC 4 cut(s) 285, 1242, 1806, 1899
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 4 cut(s) 286, 1243, 1807, 1900
Bme1390I CCNGG 4 cut(s) 582, 1219, 1460, 1589
BmgBI CACGTC 1 cut(s) 1708
BmgT120I GGNCC 4 cut(s) 7, 1214, 1527, 1845
BmiI GGNNCC 3 cut(s) 9, 1216, 1846
BmrFI CCNGG 4 cut(s) 582, 1219, 1460, 1589
BmrI ACTGGG 2 cut(s) 186, 421
BmsI GCATC 9 cut(s) 181, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
BmuI ACTGGG 2 cut(s) 186, 421
BpiI GAAGAC 1 cut(s) 1320
BpmI CTGGAG 1 cut(s) 2169
Bpu10I CCTNAGC 2 cut(s) 1237, 2201
BpuEI CTTGAG 2 cut(s) 887, 1982
BpuMI CCSGG 1 cut(s) 582
BsaBI GATNNNNATC 1 cut(s) 1026
BsaJI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BsaWI WCCGGW 1 cut(s) 1416
BsaXI ACNNNNNCTCC 4 cut(s) 358, 388, 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 181, 427
Bse3DI GCAATG 3 cut(s) 668, 1013, 1351
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 1219, 1460, 1589
BseDI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BseGI GGATG 7 cut(s) 96, 393, 415, 615, 926, 1060, 1081
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 3 cut(s) 668, 1013, 1351
BseMII CTCAG 4 cut(s) 2079, 2192, 2233, 2279
BseNI ACTGG 2 cut(s) 181, 427
BseRI GAGGAG 1 cut(s) 1630
BseXI GCAGC 4 cut(s) 296, 1253, 1817, 1885
BseYI CCCAGC 2 cut(s) 1084, 1682
BsgI GTGCAG 3 cut(s) 573, 693, 1178
Bsh1236I CGCG 2 cut(s) 318, 714
BshFI GGCC 6 cut(s) 8, 1215, 1222, 1528, 1847, 1944
BsiHKAI GWGCWC 2 cut(s) 1191, 1557
BsiSI CCGG 3 cut(s) 87, 581, 1417
BslFI GGGAC 4 cut(s) 598, 1027, 2063, 2338
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 3 cut(s) 67, 1084, 2286
BsmBI CGTCTC 1 cut(s) 2286
BsmFI GGGAC 4 cut(s) 598, 1027, 2063, 2338
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 6 cut(s) 8, 1215, 1222, 1528, 1847, 1944
Bsp1286I GDGCHC 3 cut(s) 765, 1191, 1557
Bsp1407I TGTACA 2 cut(s) 1565, 1621
Bsp143I GATC 4 cut(s) 108, 577, 1021, 1666
BspANI GGCC 6 cut(s) 8, 1215, 1222, 1528, 1847, 1944
BspCNI CTCAG 4 cut(s) 2080, 2193, 2232, 2280
BspFNI CGCG 2 cut(s) 318, 714
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 9, 1216, 1846
BspMAI CTGCAG 1 cut(s) 1903
BspPI GGATC 1 cut(s) 572
BspTI CTTAAG 1 cut(s) 790
BsrDI GCAATG 3 cut(s) 668, 1013, 1351
BsrGI TGTACA 2 cut(s) 1565, 1621
BsrI ACTGG 2 cut(s) 181, 427
BssECI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BssMI GATC 4 cut(s) 108, 577, 1021, 1666
BssT1I CCWWGG 3 cut(s) 130, 834, 1945
Bst2UI CCWGG 3 cut(s) 1219, 1460, 1589
Bst4CI ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
Bst6I CTCTTC 1 cut(s) 2269
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 1 cut(s) 680
BstAUI TGTACA 2 cut(s) 1565, 1621
BstC8I GCNNGC 6 cut(s) 61, 196, 457, 516, 841, 1680
BstDEI CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
BstF5I GGATG 7 cut(s) 96, 393, 415, 615, 926, 1060, 1081
BstFNI CGCG 2 cut(s) 318, 714
BstHHI GCGC 2 cut(s) 461, 714
BstKTI GATC 4 cut(s) 111, 580, 1024, 1669
BstMAI GTCTC 3 cut(s) 67, 1084, 2286
BstMBI GATC 4 cut(s) 108, 577, 1021, 1666
BstMWI GCNNNNNNNGC 5 cut(s) 274, 680, 1136, 1802, 1853
BstNI CCWGG 3 cut(s) 1219, 1460, 1589
BstNSI RCATGY 4 cut(s) 63, 899, 1574, 1659
BstSCI CCNGG 4 cut(s) 580, 1217, 1458, 1587
BstSFI CTRYAG 1 cut(s) 1899
BstUI CGCG 2 cut(s) 318, 714
BstV1I GCAGC 4 cut(s) 296, 1253, 1817, 1885
BstV2I GAAGAC 1 cut(s) 1320
BstX2I RGATCY 1 cut(s) 1021
BstXI CCANNNNNNTGG 2 cut(s) 1988, 2269
BstYI RGATCY 1 cut(s) 1021
BsuI GTATCC 2 cut(s) 740, 1052
BsuRI GGCC 6 cut(s) 8, 1215, 1222, 1528, 1847, 1944
BtgZI GCGATG 1 cut(s) 353
BtrI CACGTC 1 cut(s) 1708
BtsCI GGATG 7 cut(s) 96, 393, 415, 615, 926, 1060, 1081
BtsI GCAGTG 2 cut(s) 783, 942
BtsIMutI CAGTG 6 cut(s) 432, 783, 942, 1390, 1846, 2152
Cac8I GCNNGC 6 cut(s) 61, 196, 457, 516, 841, 1680
CciI TCATGA 1 cut(s) 156
CfoI GCGC 2 cut(s) 461, 714
Cfr13I GGNCC 4 cut(s) 7, 1214, 1527, 1845
CseI GACGC 1 cut(s) 2303
Csp6I GTAC 3 cut(s) 1566, 1622, 2196
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviQI GTAC 3 cut(s) 1566, 1622, 2196
DdeI CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
DpnI GATC 4 cut(s) 110, 579, 1023, 1668
DpnII GATC 4 cut(s) 108, 577, 1021, 1666
DraI TTTAAA 1 cut(s) 555
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 2269
EarI CTCTTC 1 cut(s) 2269
Ecl136II GAGCTC 1 cut(s) 1189
Eco130I CCWWGG 3 cut(s) 130, 834, 1945
Eco24I GRGCYC 2 cut(s) 765, 1191
Eco53kI GAGCTC 1 cut(s) 1189
Eco57I CTGAAG 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 1189
EcoO109I RGGNCCY 3 cut(s) 7, 1214, 1845
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 1217, 1458, 1587
EcoT14I CCWWGG 3 cut(s) 130, 834, 1945
EcoT22I ATGCAT 2 cut(s) 1657, 2142
EcoT38I GRGCYC 2 cut(s) 765, 1191
ErhI CCWWGG 3 cut(s) 130, 834, 1945
Esp3I CGTCTC 1 cut(s) 2286
FalI AAGNNNNNCTT 4 cut(s) 818, 850, 1255, 1287
FaqI GGGAC 4 cut(s) 598, 1027, 2063, 2338
FauNDI CATATG 2 cut(s) 642, 657
FblI GTMKAC 1 cut(s) 1584
Fnu4HI GCNGC 4 cut(s) 285, 1242, 1806, 1899
FokI GGATG 7 cut(s) 83, 400, 422, 622, 913, 1067, 1088
FriOI GRGCYC 2 cut(s) 765, 1191
Fsp4HI GCNGC 4 cut(s) 285, 1242, 1806, 1899
FspBI CTAG 1 cut(s) 131
GlaI GCGC 2 cut(s) 460, 713
GluI GCNGC 4 cut(s) 285, 1242, 1806, 1899
GsaI CCCAGC 2 cut(s) 1088, 1686
GsuI CTGGAG 1 cut(s) 2169
HaeIII GGCC 6 cut(s) 8, 1215, 1222, 1528, 1847, 1944
HapII CCGG 3 cut(s) 87, 581, 1417
HgaI GACGC 1 cut(s) 2303
HhaI GCGC 2 cut(s) 461, 714
Hin6I GCGC 2 cut(s) 459, 712
HinP1I GCGC 2 cut(s) 459, 712
HincII GTYRAC 2 cut(s) 1081, 1585
HindII GTYRAC 2 cut(s) 1081, 1585
HindIII AAGCTT 3 cut(s) 1264, 1772, 2264
HinfI GANTC 9 cut(s) 247, 320, 382, 637, 806, 1103, 1484, 2025, 2090
HpaI GTTAAC 1 cut(s) 1081
HpaII CCGG 3 cut(s) 87, 581, 1417
HphI GGTGA 7 cut(s) 41, 119, 587, 811, 823, 2000, 2300
Hpy188III TCNNGA 6 cut(s) 142, 157, 1694, 2050, 2186, 2339
Hpy99I CGWCG 3 cut(s) 34, 1709, 2108
HpyAV CCTTC 7 cut(s) 267, 484, 796, 796, 1202, 1878, 2116
HpyCH4III ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
HpyCH4IV ACGT 3 cut(s) 201, 1707, 2106
HpyF10VI GCNNNNNNNGC 5 cut(s) 274, 680, 1136, 1802, 1853
HpyF3I CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
HpySE526I ACGT 3 cut(s) 201, 1707, 2106
HspAI GCGC 2 cut(s) 459, 712
KspAI GTTAAC 1 cut(s) 1081
Kzo9I GATC 4 cut(s) 108, 577, 1021, 1666
LmnI GCTCC 4 cut(s) 709, 1186, 1596, 1824
Lsp1109I GCAGC 4 cut(s) 296, 1253, 1817, 1885
LweI GCATC 9 cut(s) 181, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
MaeI CTAG 1 cut(s) 131
MaeII ACGT 3 cut(s) 201, 1707, 2106
MaeIII GTNAC 8 cut(s) 257, 421, 436, 651, 1512, 1537, 1686, 2237
MalI GATC 4 cut(s) 110, 579, 1023, 1668
MboI GATC 4 cut(s) 108, 577, 1021, 1666
MboII GAAGA 9 cut(s) 1320, 1493, 1516, 1933, 1948, 2126, 2140, 2224, 2286
MfeI CAATTG 2 cut(s) 1725, 2160
MflI RGATCY 1 cut(s) 1021
MhlI GDGCHC 3 cut(s) 765, 1191, 1557
MlyI GAGTC 3 cut(s) 314, 1097, 2099
MmeI TCCRAC 3 cut(s) 12, 855, 960
MnlI CCTC 6 cut(s) 27, 62, 1205, 1608, 2196, 2228
Mph1103I ATGCAT 2 cut(s) 1657, 2142
MroXI GAANNNNTTC 1 cut(s) 2283
MslI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 3 cut(s) 87, 581, 1417
MspR9I CCNGG 4 cut(s) 582, 1219, 1460, 1589
MunI CAATTG 2 cut(s) 1725, 2160
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 3 cut(s) 1219, 1460, 1589
MvnI CGCG 2 cut(s) 318, 714
MwoI GCNNNNNNNGC 5 cut(s) 274, 680, 1136, 1802, 1853
NciI CCSGG 1 cut(s) 582
NdeI CATATG 2 cut(s) 642, 657
NdeII GATC 4 cut(s) 108, 577, 1021, 1666
NlaIV GGNNCC 3 cut(s) 9, 1216, 1846
NmuCI GTSAC 3 cut(s) 421, 1512, 2237
NsiI ATGCAT 2 cut(s) 1657, 2142
NspI RCATGY 4 cut(s) 63, 899, 1574, 1659
OliI CACNNNNGTG 1 cut(s) 132
PaeI GCATGC 1 cut(s) 63
PagI TCATGA 1 cut(s) 156
PciI ACATGT 2 cut(s) 895, 1570
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 2283
PfeI GAWTC 6 cut(s) 247, 382, 637, 806, 1484, 2025
PkrI GCNGC 4 cut(s) 286, 1243, 1807, 1900
PleI GAGTC 3 cut(s) 314, 1097, 2098
PpsI GAGTC 3 cut(s) 314, 1097, 2098
PscI ACATGT 2 cut(s) 895, 1570
PsiI TTATAA 2 cut(s) 354, 908
Psp124BI GAGCTC 1 cut(s) 1191
Psp6I CCWGG 3 cut(s) 1217, 1458, 1587
PspFI CCCAGC 2 cut(s) 1084, 1682
PspGI CCWGG 3 cut(s) 1217, 1458, 1587
PspN4I GGNNCC 3 cut(s) 9, 1216, 1846
PspPI GGNCC 4 cut(s) 7, 1214, 1527, 1845
PstI CTGCAG 1 cut(s) 1903
PsuI RGATCY 1 cut(s) 1021
RsaI GTAC 3 cut(s) 1567, 1623, 2197
RsaNI GTAC 3 cut(s) 1566, 1622, 2196
RseI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
SacI GAGCTC 1 cut(s) 1191
SalI GTCGAC 1 cut(s) 1583
SatI GCNGC 4 cut(s) 285, 1242, 1806, 1899
Sau3AI GATC 4 cut(s) 108, 577, 1021, 1666
Sau96I GGNCC 4 cut(s) 7, 1214, 1527, 1845
SchI GAGTC 3 cut(s) 314, 1097, 2099
ScrFI CCNGG 4 cut(s) 582, 1219, 1460, 1589
SduI GDGCHC 3 cut(s) 765, 1191, 1557
SfaNI GCATC 9 cut(s) 181, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
SfcI CTRYAG 1 cut(s) 1899
SmiMI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
SmlI CTYRAG 3 cut(s) 790, 866, 1961
SmoI CTYRAG 3 cut(s) 790, 866, 1961
SphI GCATGC 1 cut(s) 63
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 1191
StyD4I CCNGG 4 cut(s) 580, 1217, 1458, 1587
StyI CCWWGG 3 cut(s) 130, 834, 1945
TaaI ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
TaiI ACGT 3 cut(s) 204, 1710, 2109
TaqI TCGA 7 cut(s) 189, 207, 279, 464, 629, 765, 1584
TatI WGTACW 2 cut(s) 1565, 1621
TfiI GAWTC 6 cut(s) 247, 382, 637, 806, 1484, 2025
TscAI CASTG 6 cut(s) 439, 783, 942, 1390, 1846, 2152
TseFI GTSAC 3 cut(s) 421, 1512, 2237
TseI GCWGC 4 cut(s) 284, 1241, 1805, 1898
Tsp45I GTSAC 3 cut(s) 421, 1512, 2237
TspDTI ATGAA 9 cut(s) 161, 173, 225, 225, 318, 1286, 1766, 1934, 2225
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 6 cut(s) 439, 783, 942, 1390, 1846, 2152
Vha464I CTTAAG 1 cut(s) 790
XapI RAATTY 3 cut(s) 730, 1293, 2252
XceI RCATGY 4 cut(s) 63, 899, 1574, 1659
XcmI CCANNNNNNNNNTGG 1 cut(s) 2318
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 1584
XmnI GAANNNNTTC 1 cut(s) 2283
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 2 cut(s) 1657, 2142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.