Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0008593

Physical Location & Seq

Genomic Coordinates Reverse (-)

27132524 .. 27134875

Gene Structure 2352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018225

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGAGGCATCAAACCCTCTGACCACTTCGTCGGATTTCTACGCCTATCACCTCCAAGCATGCCTAAACAAGAGACACCCATTTGCCGGAAAATCCATCCATGCCCAGATCATCAAGTCAGGGCTTCACCTAGGTGTGTTCTTGATGAACAATCTCATGAACAATTACATCAAAACTGGGTCTTATGTCGACGCATACCACGTGTTCGATGAAATGCCTGTGAGAAACAAGTTCTCGTGGAACACGATTCTCTCTGGTTACACAAAGCAGGGAAGGCTTGATGCAGCTTGGGGAGTTTTTAATGAAATGCCTGAACGTGACTCGGTTTCGTGGACTGCGATGATCGTGGGTTATAATCAGATGGGGAGATATGGCAATGCGATTCGGATGTTCTTGGAGATGGTTTCGGATGGAGTTCCTGTGACCCAGTTCACTGTTACTAACATTCTTTCGGCATGCGCTTCGATTAAGGGTTTGGATATTGGTAGGAAGGTGCATTCCTTTGTTGTTAAGCTTGCTGTTGGCAGTTATGTTTCGGTTGGGAACTGCCTTTTAAATATGTATGCGAAATGCGGTGATCCCGGGACTGCACAGATAGTTTTTGATAGGATGAGATTGAAAAATGTATCAAGCTGGAATCATATGATTATGTTACATATGCAATGTGGTCGTGCAGACCTTGCTCTTGCACAGTTTAATCAAATGACGGAGCGCGACATTGTTTCTTGGAATTCAATGATTGCTGGATACAACCAGCATGGGCTCGATATGGAAGCAGTGGCTATCTTCTTAAGTATGGTGAAGGATTCTTTTCTAAAGCCTGATAAGTTCACCTTGGCAAGCATCTTGTCAGCTTGTGCTAACCTTGAGAAGTTGGAACTTGGAAAACAAATACATGTTCTTATTATAAGAGCTGGATTGGACACATCCGAAGCAGTGGTTAATGCTTTGATTTCAATGTATGCCAAGTCTGGTGGGTTGGAAATTGCCGAAAAGGTTTTAGAGCAATGTGGGATTTCAGATCTCAATCTTATAGCTTTTACTGCCTTATTGGATGGATACATTAAGGTCGGGGATGTTTACCCAGCCAGACAGATATTTGACTCATTGAGAGAATGTGATGTGGTAGCATGGACAGCTATGATTGTCGGTTATGTGCAGAATGGGTTGAATAATGATGCTTTGGAGCTCTTTAGGTCAATGATAGCAGGGGGCCCCAGGCCAAACAACTACACCTTAGCAGCTATGTTAAGTGTCAGTTCAAGCTTGGCTTCTTTGGATTATGGAAAACAAATTCATGCAAGTGCTATACGAACAGGAGAAGTGTCTTCAGTTTCTGTGGGCAATGCTCTAATTACCATGTATTCCAAAGCTGGAAGCATCAGTGGTGCAAAGCAAGTTTTCAATCTGATTGACAGGAATAGAGATACTGTTTCTTGGAGTTCCATGATTATTGCCCTGGCTCAACATGGTCTTGGTGAAGAATCCATTGAACTATTTGAGAAGATGCTGTCACTTGGTATTAGGCCCGACCATGTAACTTATGTTGGGGTGCTCTCTGCTTGTACACATGTGGGGTTGGTCGACCAGGGCAGGAGCTACTATAAGTTGATGAAGAGCGTACACAAGATTGAACCCACCCATAGCCATTATGCATGTATGGTTGATCTGCTTGGGCGTGCTGGGTTACTTCAAGAAGCATACGACGTCATACAAAATATGCCAATTGAACCTGATGTTATAGCTTGGGGTTCACTTTTATCTGCTTGTAAGCTTCATAAAAATGTGGATTTGGCTAAAGTTGCAGCAGACAAATTGCTCCTTATTGAACCAGACAACAGTGGGGCCTATTCAGCCCTTGCTAATTTGTATTCAGCTTGTGGAAGGTGGGCAGATGCTGCAGAAGTAAGAAAGTTAATGAAGAATAGGAGAGTGAAGAAAGGCCAAGGATTTAGTTGGCTTGAGTATCAGAACAAAGTCCATGTGTTTGGGGTTGACGATGGAGTTCATCCACAGAGAAATGGAATCTACAAGATGATGGAGAAAATCTGGAAAGAGATTAAAAATATCGGTTTTGTCCCTGATACTGAGTCAGTAGTGCATGACGTTGCAGAAGAATTGAAGGAGAAGATGCTTATGCATCACAGTGAGAAACTTGCAATTGCATTTGGGCTAATAAATACTCCAGAGAACAGTACTTTGAGGATTATGAAGAACCTCAGAGTTTGTAATGATTGTCACTCTGCCATTAAATTTATATCCAAGCTTGTGGGAAGAGAAATCATTTTGAGAGACGCTACTCGTTTTCACCATTTTAAGAATGGGACTTGTTCTTGTCGGGACTATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

784

Amino Acids

86.92

Weight (kDa)

8.18

Isoelectric Point (pI)

33.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	76 - 106	8.8e-08	PPR repeat family
PPR	PF01535	78 - 106	1.1e-06	PPR repeat
PPR_2	PF13041	107 - 154	5.8e-08	PPR repeat family
PPR_2	PF13041	238 - 288	2.3e-06	PPR repeat family
PPR_2	PF13041	374 - 417	1.3e-08	PPR repeat family
PPR_2	PF13041	475 - 522	8.4e-09	PPR repeat family
PPR	PF01535	478 - 508	3.2e-07	PPR repeat
E_motif	PF20431	593 - 655	5e-16	E motif
DYW_deaminase	PF14432	691 - 783	4.5e-44	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008593)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22070
fragaria_vesca	FvH4_6g24010 FvH4_6g24010
malus_domestica	MD01G1046800.v1.1 MD16G1282200.v1.1
prunus_persica	Prupe.3G166700_v2.0.a1
pyrus_communis	pycom01g07270
rosa_chinensis	RchiOBHm_Chr2g0115941
rosa_laevigata	RLG00000018225
rosa_multiflora	Rmu_co8514103.1_g000001 Rmu_sc0025044.1_g000002 Rmu_ssc0000197.1_g000005
rosa_roxburghii	Rroxscaffold_2G00127720 Rroxscaffold_2G00128490
rosa_rugosa	Rorug02G0201400 Rorug02G0201500 Rorug02G0201600 Rorug02G0201700
rosa_samantha	Rh2AG258200 Rh2BG269300 Rh2CG264900 Rh2DG266600
rosa_wichuraiana	Rw2G020250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	354, 908
AasI	GACNNNNNNGTC	1 cut(s)	28
AatII	GACGTC	1 cut(s)	1710
AccI	GTMKAC	2 cut(s)	189, 1584
AccII	CGCG	1 cut(s)	714
AciI	CCGC	1 cut(s)	573
AclWI	GGATC	1 cut(s)	572
AcsI	RAATTY	3 cut(s)	730, 1293, 2252
AcuI	CTGAAG	1 cut(s)	1314
AcvI	CACGTG	1 cut(s)	202
AcyI	GRCGYC	1 cut(s)	1707
AfaI	GTAC	3 cut(s)	1567, 1623, 2197
AfiI	CCNNNNNNNGG	1 cut(s)	86
AflII	CTTAAG	1 cut(s)	790
AflIII	ACRYGT	3 cut(s)	201, 895, 1570
AjnI	CCWGG	3 cut(s)	1217, 1458, 1587
AleI	CACNNNNGTG	1 cut(s)	132
Alw21I	GWGCWC	2 cut(s)	1191, 1557
Alw26I	GTCTC	2 cut(s)	67, 2286
AlwI	GGATC	1 cut(s)	572
AlwNI	CAGNNNCTG	2 cut(s)	1337, 1898
Ama87I	CYCGRG	1 cut(s)	581
AoxI	GGCC	5 cut(s)	1213, 1220, 1526, 1845, 1942
ApaI	GGGCCC	1 cut(s)	1217
ApeKI	GCWGC	4 cut(s)	284, 1241, 1805, 1898
ApoI	RAATTY	3 cut(s)	730, 1293, 2252
AspA2I	CCTAGG	1 cut(s)	130
AspLEI	GCGC	2 cut(s)	461, 714
AspS9I	GGNCC	4 cut(s)	1213, 1214, 1527, 1845
AsuC2I	CCSGG	2 cut(s)	582, 583
AsuHPI	GGTGA	7 cut(s)	41, 119, 587, 811, 823, 1490, 2300
AvaI	CYCGRG	1 cut(s)	581
AvrII	CCTAGG	1 cut(s)	130
BaeGI	GKGCMC	1 cut(s)	1217
BanII	GRGCYC	3 cut(s)	765, 1191, 1217
BauI	CACGAG	1 cut(s)	235
BbrPI	CACGTG	1 cut(s)	202
BbsI	GAAGAC	1 cut(s)	1320
Bbv12I	GWGCWC	2 cut(s)	1191, 1557
BbvI	GCAGC	4 cut(s)	296, 1253, 1817, 1885
BccI	CCATC	7 cut(s)	104, 355, 394, 404, 1049, 1994, 2032
BcgI	CGANNNNNNTGC	4 cut(s)	444, 478, 650, 684
BciT130I	CCWGG	3 cut(s)	1219, 1460, 1589
BciVI	GTATCC	2 cut(s)	740, 1052
BcnI	CCSGG	2 cut(s)	582, 583
BcoDI	GTCTC	2 cut(s)	67, 2286
BfaI	CTAG	1 cut(s)	131
BfmI	CTRYAG	1 cut(s)	1899
BfrI	CTTAAG	1 cut(s)	790
BfuI	GTATCC	2 cut(s)	740, 1052
BglII	AGATCT	1 cut(s)	1021
BisI	GCNGC	4 cut(s)	285, 1242, 1806, 1899
BlnI	CCTAGG	1 cut(s)	130
BlsI	GCNGC	4 cut(s)	286, 1243, 1807, 1900
BmcAI	AGTACT	1 cut(s)	2197
Bme1390I	CCNGG	5 cut(s)	582, 583, 1219, 1460, 1589
BmeT110I	CYCGRG	1 cut(s)	581
BmgT120I	GGNCC	4 cut(s)	1213, 1214, 1527, 1845
BmiI	GGNNCC	4 cut(s)	1214, 1215, 1216, 1846
BmrFI	CCNGG	5 cut(s)	582, 583, 1219, 1460, 1589
BmrI	ACTGGG	2 cut(s)	186, 421
BmsI	GCATC	9 cut(s)	17, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
BmuI	ACTGGG	2 cut(s)	186, 421
BpiI	GAAGAC	1 cut(s)	1320
BpmI	CTGGAG	1 cut(s)	2169
Bpu10I	CCTNAGC	1 cut(s)	1237
BpuEI	CTTGAG	2 cut(s)	887, 1982
BpuMI	CCSGG	2 cut(s)	582, 583
BsaAI	YACGTR	1 cut(s)	202
BsaBI	GATNNNNATC	1 cut(s)	1026
BsaHI	GRCGYC	1 cut(s)	1707
BsaJI	CCNNGG	7 cut(s)	130, 581, 834, 1217, 1458, 1588, 1945
BsaXI	ACNNNNNCTCC	2 cut(s)	405, 435
Bsc4I	CCNNNNNNNGG	1 cut(s)	86
Bse1I	ACTGG	2 cut(s)	181, 427
Bse3DI	GCAATG	4 cut(s)	382, 668, 1013, 1351
Bse8I	GATNNNNATC	1 cut(s)	1026
BseBI	CCWGG	3 cut(s)	1219, 1460, 1589
BseDI	CCNNGG	7 cut(s)	130, 581, 834, 1217, 1458, 1588, 1945
BseGI	GGATG	8 cut(s)	96, 393, 415, 615, 926, 1060, 1081, 2008
BseJI	GATNNNNATC	1 cut(s)	1026
BseLI	CCNNNNNNNGG	1 cut(s)	86
BseMI	GCAATG	4 cut(s)	382, 668, 1013, 1351
BseMII	CTCAG	2 cut(s)	2079, 2233
BseNI	ACTGG	2 cut(s)	181, 427
BseSI	GKGCMC	1 cut(s)	1217
BseXI	GCAGC	4 cut(s)	296, 1253, 1817, 1885
BseYI	CCCAGC	2 cut(s)	1084, 1682
BsgI	GTGCAG	3 cut(s)	573, 693, 1178
Bsh1236I	CGCG	1 cut(s)	714
BshFI	GGCC	5 cut(s)	1215, 1222, 1528, 1847, 1944
BsiHKAI	GWGCWC	2 cut(s)	1191, 1557
BsiHKCI	CYCGRG	1 cut(s)	581
BsiSI	CCGG	2 cut(s)	87, 582
BslFI	GGGAC	3 cut(s)	598, 2063, 2338
BslI	CCNNNNNNNGG	1 cut(s)	86
BsmAI	GTCTC	2 cut(s)	67, 2286
BsmBI	CGTCTC	1 cut(s)	2286
BsmFI	GGGAC	3 cut(s)	598, 2063, 2338
BsmI	GAATGC	1 cut(s)	496
BsnI	GGCC	5 cut(s)	1215, 1222, 1528, 1847, 1944
BsoBI	CYCGRG	1 cut(s)	581
Bsp120I	GGGCCC	1 cut(s)	1213
Bsp1286I	GDGCHC	4 cut(s)	765, 1191, 1217, 1557
Bsp1407I	TGTACA	1 cut(s)	1565
Bsp143I	GATC	5 cut(s)	108, 342, 577, 1021, 1666
BspACI	CCGC	1 cut(s)	573
BspANI	GGCC	5 cut(s)	1215, 1222, 1528, 1847, 1944
BspCNI	CTCAG	2 cut(s)	2080, 2232
BspFNI	CGCG	1 cut(s)	714
BspHI	TCATGA	1 cut(s)	156
BspLI	GGNNCC	4 cut(s)	1214, 1215, 1216, 1846
BspMAI	CTGCAG	1 cut(s)	1903
BspPI	GGATC	1 cut(s)	572
BspQI	GCTCTTC	1 cut(s)	1610
BspTI	CTTAAG	1 cut(s)	790
BsrDI	GCAATG	4 cut(s)	382, 668, 1013, 1351
BsrGI	TGTACA	1 cut(s)	1565
BsrI	ACTGG	2 cut(s)	181, 427
BssECI	CCNNGG	7 cut(s)	130, 581, 834, 1217, 1458, 1588, 1945
BssMI	GATC	5 cut(s)	108, 342, 577, 1021, 1666
BssNI	GRCGYC	1 cut(s)	1707
BssSI	CACGAG	1 cut(s)	235
BssT1I	CCWWGG	3 cut(s)	130, 834, 1945
Bst2BI	CACGAG	1 cut(s)	235
Bst2UI	CCWGG	3 cut(s)	1219, 1460, 1589
Bst4CI	ACNGT	6 cut(s)	436, 693, 1432, 1841, 2147, 2195
Bst6I	CTCTTC	2 cut(s)	1610, 2269
BstACI	GRCGYC	1 cut(s)	1707
BstAFI	CTTAAG	1 cut(s)	790
BstAPI	GCANNNNNTGC	2 cut(s)	680, 1898
BstAUI	TGTACA	1 cut(s)	1565
BstBAI	YACGTR	1 cut(s)	202
BstC8I	GCNNGC	5 cut(s)	61, 457, 516, 841, 1680
BstDEI	CTNAG	3 cut(s)	1237, 2088, 2219
BstF5I	GGATG	8 cut(s)	96, 393, 415, 615, 926, 1060, 1081, 2008
BstFNI	CGCG	1 cut(s)	714
BstHHI	GCGC	2 cut(s)	461, 714
BstKTI	GATC	5 cut(s)	111, 345, 580, 1024, 1669
BstMAI	GTCTC	2 cut(s)	67, 2286
BstMBI	GATC	5 cut(s)	108, 342, 577, 1021, 1666
BstMWI	GCNNNNNNNGC	7 cut(s)	274, 680, 1043, 1136, 1802, 1853, 1898
BstNI	CCWGG	3 cut(s)	1219, 1460, 1589
BstNSI	RCATGY	5 cut(s)	63, 459, 899, 1574, 1659
BstSCI	CCNGG	5 cut(s)	580, 581, 1217, 1458, 1587
BstSFI	CTRYAG	1 cut(s)	1899
BstSLI	GKGCMC	1 cut(s)	1217
BstUI	CGCG	1 cut(s)	714
BstV1I	GCAGC	4 cut(s)	296, 1253, 1817, 1885
BstV2I	GAAGAC	1 cut(s)	1320
BstX2I	RGATCY	1 cut(s)	1021
BstXI	CCANNNNNNTGG	2 cut(s)	1988, 2269
BstYI	RGATCY	1 cut(s)	1021
BsuI	GTATCC	2 cut(s)	740, 1052
BsuRI	GGCC	5 cut(s)	1215, 1222, 1528, 1847, 1944
BtgZI	GCGATG	1 cut(s)	353
BtsCI	GGATG	8 cut(s)	96, 393, 415, 615, 926, 1060, 1081, 2008
BtsI	GCAGTG	2 cut(s)	783, 942
BtsIMutI	CAGTG	6 cut(s)	432, 783, 942, 1390, 1846, 2152
Cac8I	GCNNGC	5 cut(s)	61, 457, 516, 841, 1680
CaiI	CAGNNNCTG	2 cut(s)	1337, 1898
CciI	TCATGA	1 cut(s)	156
CfoI	GCGC	2 cut(s)	461, 714
Cfr13I	GGNCC	4 cut(s)	1213, 1214, 1527, 1845
Cfr9I	CCCGGG	1 cut(s)	581
CseI	GACGC	2 cut(s)	200, 2303
Csp6I	GTAC	3 cut(s)	1566, 1622, 2196
CspCI	CAANNNNNGTGG	2 cut(s)	955, 990
CviQI	GTAC	3 cut(s)	1566, 1622, 2196
DdeI	CTNAG	3 cut(s)	1237, 2088, 2219
DpnI	GATC	5 cut(s)	110, 344, 579, 1023, 1668
DpnII	GATC	5 cut(s)	108, 342, 577, 1021, 1666
DraI	TTTAAA	1 cut(s)	555
DrdI	GACNNNNNNGTC	1 cut(s)	28
DseDI	GACNNNNNNGTC	1 cut(s)	28
Eam1104I	CTCTTC	2 cut(s)	1610, 2269
EarI	CTCTTC	2 cut(s)	1610, 2269
Ecl136II	GAGCTC	1 cut(s)	1189
Eco130I	CCWWGG	3 cut(s)	130, 834, 1945
Eco24I	GRGCYC	3 cut(s)	765, 1191, 1217
Eco53kI	GAGCTC	1 cut(s)	1189
Eco57I	CTGAAG	1 cut(s)	1314
Eco72I	CACGTG	1 cut(s)	202
Eco88I	CYCGRG	1 cut(s)	581
EcoICRI	GAGCTC	1 cut(s)	1189
EcoO109I	RGGNCCY	3 cut(s)	1213, 1214, 1845
EcoRI	GAATTC	1 cut(s)	730
EcoRII	CCWGG	3 cut(s)	1217, 1458, 1587
EcoT14I	CCWWGG	3 cut(s)	130, 834, 1945
EcoT22I	ATGCAT	2 cut(s)	1657, 2142
EcoT38I	GRGCYC	3 cut(s)	765, 1191, 1217
ErhI	CCWWGG	3 cut(s)	130, 834, 1945
Esp3I	CGTCTC	1 cut(s)	2286
FalI	AAGNNNNNCTT	4 cut(s)	818, 850, 1255, 1287
FaqI	GGGAC	3 cut(s)	598, 2063, 2338
FauNDI	CATATG	2 cut(s)	642, 657
FblI	GTMKAC	2 cut(s)	189, 1584
Fnu4HI	GCNGC	4 cut(s)	285, 1242, 1806, 1899
FokI	GGATG	8 cut(s)	83, 400, 422, 622, 913, 1067, 1088, 1995
FriOI	GRGCYC	3 cut(s)	765, 1191, 1217
Fsp4HI	GCNGC	4 cut(s)	285, 1242, 1806, 1899
FspBI	CTAG	1 cut(s)	131
GlaI	GCGC	2 cut(s)	460, 713
GluI	GCNGC	4 cut(s)	285, 1242, 1806, 1899
GsaI	CCCAGC	2 cut(s)	1088, 1686
GsuI	CTGGAG	1 cut(s)	2169
HaeIII	GGCC	5 cut(s)	1215, 1222, 1528, 1847, 1944
HapII	CCGG	2 cut(s)	87, 582
HgaI	GACGC	2 cut(s)	200, 2303
HhaI	GCGC	2 cut(s)	461, 714
Hin1I	GRCGYC	1 cut(s)	1707
Hin6I	GCGC	2 cut(s)	459, 712
HinP1I	GCGC	2 cut(s)	459, 712
HincII	GTYRAC	3 cut(s)	190, 1585, 1996
HindII	GTYRAC	3 cut(s)	190, 1585, 1996
HindIII	AAGCTT	4 cut(s)	512, 1264, 1772, 2264
HinfI	GANTC	9 cut(s)	247, 320, 382, 637, 806, 1103, 1484, 2025, 2090
HpaII	CCGG	2 cut(s)	87, 582
HphI	GGTGA	7 cut(s)	41, 119, 587, 811, 823, 1490, 2300
Hpy188III	TCNNGA	6 cut(s)	142, 157, 1694, 2050, 2186, 2339
Hpy99I	CGWCG	3 cut(s)	34, 194, 1709
HpyAV	CCTTC	5 cut(s)	267, 484, 796, 1878, 2116
HpyCH4III	ACNGT	6 cut(s)	436, 693, 1432, 1841, 2147, 2195
HpyCH4IV	ACGT	4 cut(s)	201, 316, 1707, 2106
HpyF10VI	GCNNNNNNNGC	7 cut(s)	274, 680, 1043, 1136, 1802, 1853, 1898
HpyF3I	CTNAG	3 cut(s)	1237, 2088, 2219
HpySE526I	ACGT	4 cut(s)	201, 316, 1707, 2106
Hsp92I	GRCGYC	1 cut(s)	1707
HspAI	GCGC	2 cut(s)	459, 712
Kzo9I	GATC	5 cut(s)	108, 342, 577, 1021, 1666
LguI	GCTCTTC	1 cut(s)	1610
LmnI	GCTCC	4 cut(s)	709, 1186, 1596, 1824
Lsp1109I	GCAGC	4 cut(s)	296, 1253, 1817, 1885
LweI	GCATC	9 cut(s)	17, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
MaeI	CTAG	1 cut(s)	131
MaeII	ACGT	4 cut(s)	201, 316, 1707, 2106
MaeIII	GTNAC	9 cut(s)	257, 317, 421, 436, 651, 1512, 1537, 1686, 2237
MalI	GATC	5 cut(s)	110, 344, 579, 1023, 1668
MboI	GATC	5 cut(s)	108, 342, 577, 1021, 1666
MfeI	CAATTG	2 cut(s)	1725, 2160
MflI	RGATCY	1 cut(s)	1021
MhlI	GDGCHC	4 cut(s)	765, 1191, 1217, 1557
MlyI	GAGTC	3 cut(s)	314, 1097, 2099
MmeI	TCCRAC	3 cut(s)	12, 855, 960
MnlI	CCTC	4 cut(s)	27, 62, 2196, 2228
Mph1103I	ATGCAT	2 cut(s)	1657, 2142
MslI	CAYNNNNRTG	4 cut(s)	132, 1302, 1782, 2145
MspCI	CTTAAG	1 cut(s)	790
MspI	CCGG	2 cut(s)	87, 582
MspR9I	CCNGG	5 cut(s)	582, 583, 1219, 1460, 1589
MunI	CAATTG	2 cut(s)	1725, 2160
Mva1269I	GAATGC	1 cut(s)	496
MvaI	CCWGG	3 cut(s)	1219, 1460, 1589
MvnI	CGCG	1 cut(s)	714
MwoI	GCNNNNNNNGC	7 cut(s)	274, 680, 1043, 1136, 1802, 1853, 1898
NciI	CCSGG	2 cut(s)	582, 583
NdeI	CATATG	2 cut(s)	642, 657
NdeII	GATC	5 cut(s)	108, 342, 577, 1021, 1666
NlaIV	GGNNCC	4 cut(s)	1214, 1215, 1216, 1846
NmuCI	GTSAC	4 cut(s)	317, 421, 1512, 2237
NsiI	ATGCAT	2 cut(s)	1657, 2142
NspI	RCATGY	5 cut(s)	63, 459, 899, 1574, 1659
OliI	CACNNNNGTG	1 cut(s)	132
PaeI	GCATGC	2 cut(s)	63, 459
PagI	TCATGA	1 cut(s)	156
PciI	ACATGT	2 cut(s)	895, 1570
PciSI	GCTCTTC	1 cut(s)	1610
PcsI	WCGNNNNNNNCGW	3 cut(s)	198, 242, 335
PctI	GAATGC	1 cut(s)	496
PfeI	GAWTC	6 cut(s)	247, 382, 637, 806, 1484, 2025
PkrI	GCNGC	4 cut(s)	286, 1243, 1807, 1900
PleI	GAGTC	3 cut(s)	314, 1097, 2098
PmaCI	CACGTG	1 cut(s)	202
PmlI	CACGTG	1 cut(s)	202
PpsI	GAGTC	3 cut(s)	314, 1097, 2098
Ppu21I	YACGTR	1 cut(s)	202
PscI	ACATGT	2 cut(s)	895, 1570
PsiI	TTATAA	2 cut(s)	354, 908
Psp124BI	GAGCTC	1 cut(s)	1191
Psp6I	CCWGG	3 cut(s)	1217, 1458, 1587
PspCI	CACGTG	1 cut(s)	202
PspFI	CCCAGC	2 cut(s)	1084, 1682
PspGI	CCWGG	3 cut(s)	1217, 1458, 1587
PspN4I	GGNNCC	4 cut(s)	1214, 1215, 1216, 1846
PspOMI	GGGCCC	1 cut(s)	1213
PspPI	GGNCC	4 cut(s)	1213, 1214, 1527, 1845
PstI	CTGCAG	1 cut(s)	1903
PstNI	CAGNNNCTG	2 cut(s)	1337, 1898
PsuI	RGATCY	1 cut(s)	1021
RsaI	GTAC	3 cut(s)	1567, 1623, 2197
RsaNI	GTAC	3 cut(s)	1566, 1622, 2196
RseI	CAYNNNNRTG	4 cut(s)	132, 1302, 1782, 2145
SacI	GAGCTC	1 cut(s)	1191
SalI	GTCGAC	2 cut(s)	188, 1583
SapI	GCTCTTC	1 cut(s)	1610
SatI	GCNGC	4 cut(s)	285, 1242, 1806, 1899
Sau3AI	GATC	5 cut(s)	108, 342, 577, 1021, 1666
Sau96I	GGNCC	4 cut(s)	1213, 1214, 1527, 1845
ScaI	AGTACT	1 cut(s)	2197
SchI	GAGTC	3 cut(s)	314, 1097, 2099
ScrFI	CCNGG	5 cut(s)	582, 583, 1219, 1460, 1589
SduI	GDGCHC	4 cut(s)	765, 1191, 1217, 1557
SfaNI	GCATC	9 cut(s)	17, 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
SfcI	CTRYAG	1 cut(s)	1899
SmaI	CCCGGG	1 cut(s)	583
SmiMI	CAYNNNNRTG	4 cut(s)	132, 1302, 1782, 2145
SmlI	CTYRAG	3 cut(s)	790, 866, 1961
SmoI	CTYRAG	3 cut(s)	790, 866, 1961
SphI	GCATGC	2 cut(s)	63, 459
SsiI	CCGC	1 cut(s)	573
SspMI	CTAG	1 cut(s)	131
SstI	GAGCTC	1 cut(s)	1191
StyD4I	CCNGG	5 cut(s)	580, 581, 1217, 1458, 1587
StyI	CCWWGG	3 cut(s)	130, 834, 1945
TaaI	ACNGT	6 cut(s)	436, 693, 1432, 1841, 2147, 2195
TaiI	ACGT	4 cut(s)	204, 319, 1710, 2109
TaqI	TCGA	5 cut(s)	189, 207, 464, 765, 1584
TatI	WGTACW	2 cut(s)	1565, 2195
TfiI	GAWTC	6 cut(s)	247, 382, 637, 806, 1484, 2025
TscAI	CASTG	6 cut(s)	439, 783, 942, 1390, 1846, 2152
TseFI	GTSAC	4 cut(s)	317, 421, 1512, 2237
TseI	GCWGC	4 cut(s)	284, 1241, 1805, 1898
Tsp45I	GTSAC	4 cut(s)	317, 421, 1512, 2237
TspGWI	ACGGA	1 cut(s)	722
TspMI	CCCGGG	1 cut(s)	581
TspRI	CASTG	6 cut(s)	439, 783, 942, 1390, 1846, 2152
Vha464I	CTTAAG	1 cut(s)	790
XapI	RAATTY	3 cut(s)	730, 1293, 2252
XceI	RCATGY	5 cut(s)	63, 459, 899, 1574, 1659
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2318
XmaI	CCCGGG	1 cut(s)	581
XmaJI	CCTAGG	1 cut(s)	130
XmiI	GTMKAC	2 cut(s)	189, 1584
XspI	CTAG	1 cut(s)	131
ZraI	GACGTC	1 cut(s)	1708
ZrmI	AGTACT	1 cut(s)	2197
Zsp2I	ATGCAT	2 cut(s)	1657, 2142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.