Rroxscaffold_2G00127720

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008593
Physical Location & Seq
Reverse (-)
63311731 .. 63317395
5665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127720.1

Sequence Viewer

Length: 2352 bp
ATGCAGGCACCAAACCCTCTTACCTCTTCGTCGGATTTCTATGCCTATCACCTCCAAGCATGTCTAAACAAGAGAGACCCATTTGCCGGAAAATCCATCCATGCCCAGATCATCAAGTCAGGGCTTCACCTAGGTGTGTTCTTGATGAACAATCTCATGAACTATTACAGCAAAACTGGGTCTTATGTCGACGCACGCAACGTGTTCGATGAAATGCCTGTGAGAAACAAGTTCTCGTGGAACACCATTCTCTCTGGTTACACAAAGCAGGGAAGGCTTGATGCAGCTTGGGGAGTTTTTAATGAAATGCCTGAACGTGACTCGGTTTCGTGGACTGCGATGATCGTGGGTTATAATCAGATGGGGAGATATGCTAATGCGATTCGGATGTTCTTGGACATGGTTTCGGATGGAGTTCCTGTGACCCAGTTCACTGTTACTAACATTCTTTCGGCATGCGCTTCGATTAAGGGTTTGGATATTGGTAGGAAGGTGCATTCGTTTGTTGTTAAGCTTGCTGTTGGCAGTTATGTTTCGGTTGGGAACTGCCTTTTAAATATGTATGCGAAATGCGGTGATCCCGGGACTGCACAGATTGTTTTTGATAGGATGAGATTGAAAAATGTATCGAGCTGGAATCATATGATTATGTTACATATGCAATGTGGTCGTGCAGACCTTGCTCTTGCACAGTTTAATCAAATGACGGAGCGAGACATTGTTTCTTGGAATTCAATGATTGCTGGATACAACCAGCATGGGCTCGATATGGAAGCAGTGGCTACCTTCTTAAGCATGGGGAAGGATTCTTTTCTAAAGCCTGATAAGTTCACCTTGGCAAGCATCTTGTCAGCTTGTGCTAACCTTGAGAAGTTGGAACTTGGAAAACAAATACATGTTCTTATTATAAGAGCTGGATTGGACACATCCGAAGCAGTGGTTAATGCTTTGATTTCAATGTATGCCAAGTCTGGTGGGTTGGAAATTGCTGAAAAGGTTTTAGAGCAATGTGGGACTTCAGATCTCAATCTCATAGCTTTTACTGCCTTATTGGATGGATACATTAAGGTTGGGGATGTTAACCCAGCGAGACAGATATTTGACTCATTGAGAGAATGTGATGTGGTAGCATGGACAGCTATGATTGTCGGTTATGTGCAGAATGGGTTGAATAACGATGCTTTGGAGCTCTTTAGGTCAATGATAGCAGGAGGCCCCAGGCCAAACAACTACACCTTAGCAGCCATGTTAAGTGTCAGTTCAAGCTTGGCTTCTTTGGATTATGGAAAACAAATTCATGCAAGTGCTATACGAACAGGAGAAGTGTCTTCAGTTTCTGTGGGCAATGCTCTAATTACCATGTATTCCAAAGCTGGAAGCATCAGTGGTGCAAAGCAAGTTTTCAATCTGATTGACCGGAATAGAGATACTGTTTCTTGGAGTTCCATGATTATAGCCCTGGCTCAACATGGTCTTGGGGAAGACTCCATTGAACTATTTGAGAAGATGCTGTCACTTGGTATTCGGCCCGACCATATAACTTATGTTGGGGTGCTCTCTGCTTGTACACATGTGGGGTTGGTCGACCAGGGCAGGAGCTACTATAATTTGATGAGGAGCGTACACAAGATTGAACCCACCCATAGCCATTATGCATGTATGGTTGATCTGCTTGGACGTGCTGGGTTACTTCAAGAAGCATACAACATGATACAAAATATGCCAATTGAACCTGATGTAATAGCTTGGGGTTCACTTTTATCTGCTTGTAAGCTTCATAAAAATGTGGATTTGGCTAAAGTTGCCGCAGATAAATTGCTCCTTATTGAACCAGACAACAGTGGGGCCTATTCAGCCCTTGCTAATTTGTATTCAGCTTGTGGAAGGTGGGCAGATGCTGCAGAAGTAAGAAAGCTAATGAAGAATAGGAGAGTGAAGAAAGGCCAAGGATTTAGTTGGCTTGAGTATCAGAACAAAGTTCATGTGTTTGGGGTTGACGATGGAGTTCATCCACAGAGAGATGGAATCTACAAGATGATGGAGAAAATCTGGAAAGAGATTAAAAATATAGGTTTTGTCCCTGATACTGAGTCAGTATTGCACGACGTTGCAGAAGAATTGAAGGAGAAGATGCTTATGCATCACAGTGAGAAACTTGCAATTGCATTTGGGCTAATAAATACTCCAGAGAACAGTACGCTGAGGATTATGAAGAACCTCAGAGTTTGTAATGATTGTCACTCTGCCATTAAATTTATATCCAAGCTTGTGGGAAGAGAAATCATTCTGAGAGACGCTACTCGTTTTCACCATTTTAAGAATGGGACTTGTTCTTGTCGGGACTATTGGTAG

Protein Analysis

783

Amino Acids

86.83

Weight (kDa)

8.19

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 106 8.8e-08 PPR repeat family
PPR PF01535 78 - 106 1.1e-06 PPR repeat
PPR_2 PF13041 107 - 154 6.5e-08 PPR repeat family
PPR_2 PF13041 374 - 417 1.3e-08 PPR repeat family
PPR_2 PF13041 475 - 522 2.7e-08 PPR repeat family
PPR PF01535 478 - 508 1.2e-06 PPR repeat
E_motif PF20431 593 - 655 5e-16 E motif
DYW_deaminase PF14432 691 - 783 3e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 354, 908
AccB1I GGYRCC 1 cut(s) 7
AccI GTMKAC 2 cut(s) 189, 1584
AciI CCGC 2 cut(s) 573, 1806
AclWI GGATC 1 cut(s) 572
AcsI RAATTY 3 cut(s) 730, 1293, 2252
AcuI CTGAAG 2 cut(s) 1002, 1314
AfaI GTAC 3 cut(s) 1567, 1623, 2197
AfiI CCNNNNNNNGG 1 cut(s) 86
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 3 cut(s) 201, 895, 1570
AjiI CACGTC 1 cut(s) 1679
AjnI CCWGG 3 cut(s) 1217, 1458, 1587
AleI CACNNNNGTG 1 cut(s) 132
Alw21I GWGCWC 2 cut(s) 1191, 1557
Alw26I GTCTC 4 cut(s) 69, 708, 1084, 2286
AlwI GGATC 1 cut(s) 572
AlwNI CAGNNNCTG 2 cut(s) 1337, 1898
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 5 cut(s) 1213, 1220, 1526, 1845, 1942
ApeKI GCWGC 3 cut(s) 284, 1241, 1898
ApoI RAATTY 3 cut(s) 730, 1293, 2252
Asp700I GAANNNNTTC 1 cut(s) 2283
AspA2I CCTAGG 1 cut(s) 130
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 3 cut(s) 1214, 1527, 1845
AsuC2I CCSGG 2 cut(s) 582, 583
AsuHPI GGTGA 5 cut(s) 41, 119, 587, 823, 2300
AvaI CYCGRG 1 cut(s) 581
AvrII CCTAGG 1 cut(s) 130
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 2 cut(s) 765, 1191
BauI CACGAG 1 cut(s) 235
BbsI GAAGAC 2 cut(s) 1320, 1488
Bbv12I GWGCWC 2 cut(s) 1191, 1557
BbvCI CCTCAGC 1 cut(s) 2201
BbvI GCAGC 3 cut(s) 296, 1253, 1885
BccI CCATC 7 cut(s) 104, 355, 404, 1049, 1994, 2015, 2032
BcgI CGANNNNNNTGC 6 cut(s) 187, 221, 444, 478, 650, 684
BciT130I CCWGG 3 cut(s) 1219, 1460, 1589
BciVI GTATCC 2 cut(s) 740, 1052
BcnI CCSGG 2 cut(s) 582, 583
BcoDI GTCTC 4 cut(s) 69, 708, 1084, 2286
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 1899
BfrI CTTAAG 1 cut(s) 790
BfuI GTATCC 2 cut(s) 740, 1052
BglII AGATCT 1 cut(s) 1021
BisI GCNGC 4 cut(s) 285, 1242, 1806, 1899
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 4 cut(s) 286, 1243, 1807, 1900
Bme1390I CCNGG 5 cut(s) 582, 583, 1219, 1460, 1589
BmeT110I CYCGRG 1 cut(s) 581
BmgBI CACGTC 1 cut(s) 1679
BmgT120I GGNCC 3 cut(s) 1214, 1527, 1845
BmiI GGNNCC 3 cut(s) 9, 1216, 1846
BmrFI CCNGG 5 cut(s) 582, 583, 1219, 1460, 1589
BmrI ACTGGG 2 cut(s) 186, 421
BmsI GCATC 8 cut(s) 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
BmuI ACTGGG 2 cut(s) 186, 421
BpiI GAAGAC 2 cut(s) 1320, 1488
BpmI CTGGAG 1 cut(s) 2169
Bpu10I CCTNAGC 2 cut(s) 1237, 2201
BpuEI CTTGAG 2 cut(s) 887, 1982
BpuMI CCSGG 2 cut(s) 582, 583
BsaBI GATNNNNATC 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BsaWI WCCGGW 1 cut(s) 1416
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 181, 427
Bse3DI GCAATG 3 cut(s) 668, 1013, 1351
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 1219, 1460, 1589
BseDI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BseGI GGATG 8 cut(s) 96, 393, 415, 615, 926, 1060, 1081, 2008
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 3 cut(s) 668, 1013, 1351
BseMII CTCAG 4 cut(s) 2079, 2192, 2233, 2279
BseNI ACTGG 2 cut(s) 181, 427
BseRI GAGGAG 1 cut(s) 1630
BseXI GCAGC 3 cut(s) 296, 1253, 1885
BseYI CCCAGC 2 cut(s) 1084, 1682
BsgI GTGCAG 3 cut(s) 573, 693, 1178
BshFI GGCC 5 cut(s) 1215, 1222, 1528, 1847, 1944
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 2 cut(s) 1191, 1557
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 3 cut(s) 87, 582, 1417
BslFI GGGAC 4 cut(s) 598, 1027, 2063, 2338
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 4 cut(s) 69, 708, 1084, 2286
BsmBI CGTCTC 1 cut(s) 2286
BsmFI GGGAC 4 cut(s) 598, 1027, 2063, 2338
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 5 cut(s) 1215, 1222, 1528, 1847, 1944
Bso31I GGTCTC 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 3 cut(s) 765, 1191, 1557
Bsp1407I TGTACA 1 cut(s) 1565
Bsp143I GATC 5 cut(s) 108, 342, 577, 1021, 1666
BspACI CCGC 2 cut(s) 573, 1806
BspANI GGCC 5 cut(s) 1215, 1222, 1528, 1847, 1944
BspCNI CTCAG 4 cut(s) 2080, 2193, 2232, 2280
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 9, 1216, 1846
BspMAI CTGCAG 1 cut(s) 1903
BspPI GGATC 1 cut(s) 572
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 790
BspTNI GGTCTC 1 cut(s) 69
BsrDI GCAATG 3 cut(s) 668, 1013, 1351
BsrGI TGTACA 1 cut(s) 1565
BsrI ACTGG 2 cut(s) 181, 427
BssECI CCNNGG 7 cut(s) 130, 581, 834, 1217, 1458, 1588, 1945
BssMI GATC 5 cut(s) 108, 342, 577, 1021, 1666
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 3 cut(s) 130, 834, 1945
Bst2BI CACGAG 1 cut(s) 235
Bst2UI CCWGG 3 cut(s) 1219, 1460, 1589
Bst4CI ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
Bst6I CTCTTC 2 cut(s) 31, 2269
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 2 cut(s) 680, 1898
BstAUI TGTACA 1 cut(s) 1565
BstC8I GCNNGC 5 cut(s) 6, 196, 457, 516, 841
BstDEI CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
BstF5I GGATG 8 cut(s) 96, 393, 415, 615, 926, 1060, 1081, 2008
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 5 cut(s) 111, 345, 580, 1024, 1669
BstMAI GTCTC 4 cut(s) 69, 708, 1084, 2286
BstMBI GATC 5 cut(s) 108, 342, 577, 1021, 1666
BstMWI GCNNNNNNNGC 7 cut(s) 274, 680, 1043, 1136, 1802, 1853, 1898
BstNI CCWGG 3 cut(s) 1219, 1460, 1589
BstNSI RCATGY 5 cut(s) 63, 459, 899, 1574, 1659
BstSCI CCNGG 5 cut(s) 580, 581, 1217, 1458, 1587
BstSFI CTRYAG 1 cut(s) 1899
BstV1I GCAGC 3 cut(s) 296, 1253, 1885
BstV2I GAAGAC 2 cut(s) 1320, 1488
BstX2I RGATCY 1 cut(s) 1021
BstXI CCANNNNNNTGG 1 cut(s) 2269
BstYI RGATCY 1 cut(s) 1021
BsuI GTATCC 2 cut(s) 740, 1052
BsuRI GGCC 5 cut(s) 1215, 1222, 1528, 1847, 1944
BtgZI GCGATG 1 cut(s) 353
BtrI CACGTC 1 cut(s) 1679
BtsCI GGATG 8 cut(s) 96, 393, 415, 615, 926, 1060, 1081, 2008
BtsI GCAGTG 2 cut(s) 783, 942
BtsIMutI CAGTG 6 cut(s) 432, 783, 942, 1390, 1846, 2152
Cac8I GCNNGC 5 cut(s) 6, 196, 457, 516, 841
CaiI CAGNNNCTG 2 cut(s) 1337, 1898
CciI TCATGA 1 cut(s) 156
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 3 cut(s) 1214, 1527, 1845
Cfr9I CCCGGG 1 cut(s) 581
CseI GACGC 2 cut(s) 200, 2303
Csp6I GTAC 3 cut(s) 1566, 1622, 2196
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviQI GTAC 3 cut(s) 1566, 1622, 2196
DdeI CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
DpnI GATC 5 cut(s) 110, 344, 579, 1023, 1668
DpnII GATC 5 cut(s) 108, 342, 577, 1021, 1666
DraI TTTAAA 1 cut(s) 555
Eam1104I CTCTTC 2 cut(s) 31, 2269
EarI CTCTTC 2 cut(s) 31, 2269
Ecl136II GAGCTC 1 cut(s) 1189
Eco130I CCWWGG 3 cut(s) 130, 834, 1945
Eco24I GRGCYC 2 cut(s) 765, 1191
Eco31I GGTCTC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 1189
Eco57I CTGAAG 2 cut(s) 1002, 1314
Eco88I CYCGRG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 1189
EcoO109I RGGNCCY 2 cut(s) 1214, 1845
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 1217, 1458, 1587
EcoT14I CCWWGG 3 cut(s) 130, 834, 1945
EcoT22I ATGCAT 2 cut(s) 1657, 2142
EcoT38I GRGCYC 2 cut(s) 765, 1191
ErhI CCWWGG 3 cut(s) 130, 834, 1945
Esp3I CGTCTC 1 cut(s) 2286
FalI AAGNNNNNCTT 4 cut(s) 818, 850, 1255, 1287
FaqI GGGAC 4 cut(s) 598, 1027, 2063, 2338
FauNDI CATATG 2 cut(s) 642, 657
FblI GTMKAC 2 cut(s) 189, 1584
Fnu4HI GCNGC 4 cut(s) 285, 1242, 1806, 1899
FokI GGATG 8 cut(s) 83, 400, 422, 622, 913, 1067, 1088, 1995
FriOI GRGCYC 2 cut(s) 765, 1191
Fsp4HI GCNGC 4 cut(s) 285, 1242, 1806, 1899
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 460
GluI GCNGC 4 cut(s) 285, 1242, 1806, 1899
GsaI CCCAGC 2 cut(s) 1088, 1686
GsuI CTGGAG 1 cut(s) 2169
HaeIII GGCC 5 cut(s) 1215, 1222, 1528, 1847, 1944
HapII CCGG 3 cut(s) 87, 582, 1417
HgaI GACGC 2 cut(s) 200, 2303
HhaI GCGC 1 cut(s) 461
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 4 cut(s) 190, 1081, 1585, 1996
HindII GTYRAC 4 cut(s) 190, 1081, 1585, 1996
HindIII AAGCTT 4 cut(s) 512, 1264, 1772, 2264
HinfI GANTC 8 cut(s) 320, 382, 637, 806, 1103, 1484, 2025, 2090
HpaI GTTAAC 1 cut(s) 1081
HpaII CCGG 3 cut(s) 87, 582, 1417
HphI GGTGA 5 cut(s) 41, 119, 587, 823, 2300
Hpy188III TCNNGA 6 cut(s) 142, 157, 1694, 2050, 2186, 2339
Hpy99I CGWCG 3 cut(s) 34, 194, 2108
HpyAV CCTTC 6 cut(s) 267, 484, 796, 796, 1878, 2116
HpyCH4III ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
HpyCH4IV ACGT 4 cut(s) 201, 316, 1678, 2106
HpyF10VI GCNNNNNNNGC 7 cut(s) 274, 680, 1043, 1136, 1802, 1853, 1898
HpyF3I CTNAG 5 cut(s) 1237, 2088, 2201, 2219, 2288
HpySE526I ACGT 4 cut(s) 201, 316, 1678, 2106
HspAI GCGC 1 cut(s) 459
KspAI GTTAAC 1 cut(s) 1081
Kzo9I GATC 5 cut(s) 108, 342, 577, 1021, 1666
LmnI GCTCC 5 cut(s) 709, 1186, 1596, 1617, 1824
Lsp1109I GCAGC 3 cut(s) 296, 1253, 1885
LweI GCATC 8 cut(s) 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
MaeI CTAG 1 cut(s) 131
MaeII ACGT 4 cut(s) 201, 316, 1678, 2106
MaeIII GTNAC 8 cut(s) 257, 317, 421, 436, 651, 1512, 1686, 2237
MalI GATC 5 cut(s) 110, 344, 579, 1023, 1668
MboI GATC 5 cut(s) 108, 342, 577, 1021, 1666
MfeI CAATTG 2 cut(s) 1725, 2160
MflI RGATCY 1 cut(s) 1021
MhlI GDGCHC 3 cut(s) 765, 1191, 1557
MlyI GAGTC 4 cut(s) 314, 1097, 1478, 2099
MmeI TCCRAC 3 cut(s) 12, 855, 960
MnlI CCTC 7 cut(s) 27, 34, 62, 1205, 1608, 2196, 2228
Mph1103I ATGCAT 2 cut(s) 1657, 2142
MroXI GAANNNNTTC 1 cut(s) 2283
MslI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 3 cut(s) 87, 582, 1417
MspR9I CCNGG 5 cut(s) 582, 583, 1219, 1460, 1589
MunI CAATTG 2 cut(s) 1725, 2160
Mva1269I GAATGC 1 cut(s) 496
MvaI CCWGG 3 cut(s) 1219, 1460, 1589
MwoI GCNNNNNNNGC 7 cut(s) 274, 680, 1043, 1136, 1802, 1853, 1898
NciI CCSGG 2 cut(s) 582, 583
NdeI CATATG 2 cut(s) 642, 657
NdeII GATC 5 cut(s) 108, 342, 577, 1021, 1666
NlaIV GGNNCC 3 cut(s) 9, 1216, 1846
NmuCI GTSAC 4 cut(s) 317, 421, 1512, 2237
NsiI ATGCAT 2 cut(s) 1657, 2142
NspI RCATGY 5 cut(s) 63, 459, 899, 1574, 1659
OliI CACNNNNGTG 1 cut(s) 132
PaeI GCATGC 1 cut(s) 459
PagI TCATGA 1 cut(s) 156
PciI ACATGT 2 cut(s) 895, 1570
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 335
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 2283
PfeI GAWTC 4 cut(s) 382, 637, 806, 2025
PkrI GCNGC 4 cut(s) 286, 1243, 1807, 1900
PleI GAGTC 4 cut(s) 314, 1097, 1478, 2098
PpsI GAGTC 4 cut(s) 314, 1097, 1478, 2098
PscI ACATGT 2 cut(s) 895, 1570
PsiI TTATAA 2 cut(s) 354, 908
Psp124BI GAGCTC 1 cut(s) 1191
Psp6I CCWGG 3 cut(s) 1217, 1458, 1587
PspFI CCCAGC 2 cut(s) 1084, 1682
PspGI CCWGG 3 cut(s) 1217, 1458, 1587
PspN4I GGNNCC 3 cut(s) 9, 1216, 1846
PspPI GGNCC 3 cut(s) 1214, 1527, 1845
PstI CTGCAG 1 cut(s) 1903
PstNI CAGNNNCTG 2 cut(s) 1337, 1898
PsuI RGATCY 1 cut(s) 1021
RsaI GTAC 3 cut(s) 1567, 1623, 2197
RsaNI GTAC 3 cut(s) 1566, 1622, 2196
RseI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
SacI GAGCTC 1 cut(s) 1191
SalI GTCGAC 2 cut(s) 188, 1583
SatI GCNGC 4 cut(s) 285, 1242, 1806, 1899
Sau3AI GATC 5 cut(s) 108, 342, 577, 1021, 1666
Sau96I GGNCC 3 cut(s) 1214, 1527, 1845
SchI GAGTC 4 cut(s) 314, 1097, 1478, 2099
ScrFI CCNGG 5 cut(s) 582, 583, 1219, 1460, 1589
SduI GDGCHC 3 cut(s) 765, 1191, 1557
SfaNI GCATC 8 cut(s) 271, 852, 1168, 1389, 1497, 1885, 2121, 2149
SfcI CTRYAG 1 cut(s) 1899
SmaI CCCGGG 1 cut(s) 583
SmiMI CAYNNNNRTG 4 cut(s) 132, 1302, 1782, 2145
SmlI CTYRAG 3 cut(s) 790, 866, 1961
SmoI CTYRAG 3 cut(s) 790, 866, 1961
SphI GCATGC 1 cut(s) 459
SsiI CCGC 2 cut(s) 573, 1806
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 1191
StyD4I CCNGG 5 cut(s) 580, 581, 1217, 1458, 1587
StyI CCWWGG 3 cut(s) 130, 834, 1945
TaaI ACNGT 6 cut(s) 436, 693, 1432, 1841, 2147, 2195
TaiI ACGT 4 cut(s) 204, 319, 1681, 2109
TaqI TCGA 6 cut(s) 189, 207, 464, 629, 765, 1584
TatI WGTACW 1 cut(s) 1565
TauI GCSGC 1 cut(s) 1808
TfiI GAWTC 4 cut(s) 382, 637, 806, 2025
TscAI CASTG 6 cut(s) 439, 783, 942, 1390, 1846, 2152
TseFI GTSAC 4 cut(s) 317, 421, 1512, 2237
TseI GCWGC 3 cut(s) 284, 1241, 1898
Tsp45I GTSAC 4 cut(s) 317, 421, 1512, 2237
TspGWI ACGGA 1 cut(s) 722
TspMI CCCGGG 1 cut(s) 581
TspRI CASTG 6 cut(s) 439, 783, 942, 1390, 1846, 2152
Vha464I CTTAAG 1 cut(s) 790
XapI RAATTY 3 cut(s) 730, 1293, 2252
XceI RCATGY 5 cut(s) 63, 459, 899, 1574, 1659
XcmI CCANNNNNNNNNTGG 1 cut(s) 2318
XmaI CCCGGG 1 cut(s) 581
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 189, 1584
XmnI GAANNNNTTC 1 cut(s) 2283
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 2 cut(s) 1657, 2142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.