pycom01g07270

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008593
Physical Location & Seq
Forward (+)
8087101 .. 8088495
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07270.1

Sequence Viewer

Length: 1395 bp
ATGTATGCTAAATCTGGTGGGTTGGCAATTGCTCAAAAGATTTTAGAGCAATGCGTGACTTCCGATCTCAATGTTATAGCTTTTACCGCCCTATTGCATGGGTACATAAAGCTTGGGGATATAATGCCAGCCAGGCAGATATCTGACTCGTTGAGAGACCGTGACGTGGTTGCATGGACAGCAATGATTTTTGGTTATGTACAGAATGGGTTGAATAATGATGCTTTGGAGCTCTTCATGTCAATGATAAAACAAGGCCCCAAGCCGAACAGCTATACCTTAGCAGCTATGTTAAGTGTCAGTTCGAGCTTGGCTTCTTTAGACTATGGAAAACAAATTCATGCAAGTGCCATGAGGTTGGGGGAAGTGCATTCAGTTTCTGTAAGCAATGCTCTAATTTCAATGTATTCCAAAGCTGGAAGCATCAGTGGTGCAAAGAAAGTATTCAATTTGATTTACTTGAATAGAGATACGGTTTCTTGGACTTCCATGATCATAGCCCTGGCTCAACATGTGCACAGGGAAGATGCCATTGAACTGTTTGAGAAGATGCTGTCACATGGTATTAAGCCTGACCATATAACTTATGTCGGCATGCTCTCTGCCTGTATACATATGGGATTGGTAGAACAGGGCAGGAGCTACTATAATTTAATGAGGAACGTCCACAAAATTGAACCCACCCATAGCCAGTATGCATGCATGATTGATCTGCTTGGGTGCTCTGGATTGCTTCAAGAAGCATTCAATTTTATACAAAGTTTGCCAGTTGAACCAGATGTTATAGCTTGGGGTTCACTTTTATCTTGTTGTAAGGTTCATAAAAATGTAGATGTAGCAAAGGTTGCAGCTGAAAGATTGCTGCTTATTGATCCAGACAACAGTGGGGCCTACTCAGCCCTTGCTAATCTGTATTCAGCTTGTGGAAGATGGGAAGAAGCTGCACAAGTTAGAAGGTCAATGAAGAATAAGGGAGTGAAGAAAGAACAAGGATTTAGTTGGATTCAGATTCAAAACAAAGTTCATGTGTTTGGGGTTGATGATGGAGTTCACATGCAGAAAGAGGCAATCTACAAGATGATGGAAAAGATATGGAAAGAGATTAAAAATATGGGTTTTGTTCCCGACACTGACTCAGTATTGCATGATGTTGGTGAAGAAGTGAAGGAGAAGATAATTAAACATCACAGTGAGAAACTTGCCATTGCATTTGGGCTAATAAATACTCCAGGGAACACGACGTTGAGGATCATGAAGAACCTTAGAGTTTGTAATGATTGTCATTCTGCCATCAAATTTATTTCCAAGCTTGTGGGCAGAGAAATCATTTTGAGAGACACTACTCGTTTTCACCATTTTAAGAACGAGCAGTGTTCTTGTCGGGACTATTGGTAG

Protein Analysis

465

Amino Acids

51.93

Weight (kDa)

8.5

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 54 - 98 1.2e-10 PPR repeat family
PPR PF01535 57 - 86 3.5e-06 PPR repeat
PPR_2 PF13041 156 - 204 4.5e-11 PPR repeat family
E_motif PF20431 274 - 336 6.8e-18 E motif
DYW_deaminase PF14432 372 - 464 2.4e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 610
AciI CCGC 1 cut(s) 87
AclWI GGATC 2 cut(s) 866, 1256
AcsI RAATTY 2 cut(s) 336, 1295
AfaI GTAC 2 cut(s) 104, 201
AfiI CCNNNNNNNGG 1 cut(s) 166
AflIII ACRYGT 1 cut(s) 511
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 3 cut(s) 131, 501, 1228
Alw21I GWGCWC 3 cut(s) 234, 519, 725
Alw26I GTCTC 2 cut(s) 150, 1329
Alw44I GTGCAC 1 cut(s) 515
AlwI GGATC 2 cut(s) 866, 1256
AlwNI CAGNNNCTG 1 cut(s) 380
AoxI GGCC 2 cut(s) 256, 888
ApaLI GTGCAC 1 cut(s) 515
ApeKI GCWGC 4 cut(s) 284, 848, 862, 941
ApoI RAATTY 2 cut(s) 336, 1295
Asp700I GAANNNNTTC 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 257, 888
AsuHPI GGTGA 2 cut(s) 1166, 1343
BaeGI GKGCMC 1 cut(s) 519
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 3 cut(s) 234, 519, 725
BbvI GCAGC 4 cut(s) 296, 849, 860, 928
BccI CCATC 4 cut(s) 924, 1037, 1075, 1298
BciT130I CCWGG 3 cut(s) 133, 503, 1230
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 2 cut(s) 150, 1329
BglI GCCNNNNNGGC 1 cut(s) 133
BisI GCNGC 4 cut(s) 285, 849, 863, 942
BlsI GCNGC 4 cut(s) 286, 850, 864, 943
Bme1390I CCNGG 3 cut(s) 133, 503, 1230
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 257, 888
BmiI GGNNCC 2 cut(s) 259, 889
BmrFI CCNGG 3 cut(s) 133, 503, 1230
BmsI GCATC 4 cut(s) 211, 432, 517, 540
BpmI CTGGAG 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 501, 1229
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 691, 767
Bse3DI GCAATG 4 cut(s) 56, 189, 394, 1203
BseBI CCWGG 3 cut(s) 133, 503, 1230
BseDI CCNNGG 2 cut(s) 501, 1229
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 4 cut(s) 56, 189, 394, 1203
BseMII CTCAG 2 cut(s) 909, 1149
BseNI ACTGG 2 cut(s) 691, 767
BseSI GKGCMC 1 cut(s) 519
BseXI GCAGC 4 cut(s) 296, 849, 860, 928
BsgI GTGCAG 1 cut(s) 927
BshFI GGCC 2 cut(s) 258, 890
BsiHKAI GWGCWC 3 cut(s) 234, 519, 725
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 2 cut(s) 150, 1329
BsmI GAATGC 2 cut(s) 370, 743
BsnI GGCC 2 cut(s) 258, 890
Bso31I GGTCTC 1 cut(s) 150
Bsp1286I GDGCHC 3 cut(s) 234, 519, 725
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 5 cut(s) 64, 492, 709, 871, 1248
BspACI CCGC 1 cut(s) 87
BspANI GGCC 2 cut(s) 258, 890
BspCNI CTCAG 2 cut(s) 908, 1148
BspHI TCATGA 1 cut(s) 1251
BspLI GGNNCC 2 cut(s) 259, 889
BspPI GGATC 2 cut(s) 866, 1256
BspQI GCTCTTC 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 150
BsrDI GCAATG 4 cut(s) 56, 189, 394, 1203
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 2 cut(s) 691, 767
BssECI CCNNGG 2 cut(s) 501, 1229
BssMI GATC 5 cut(s) 64, 492, 709, 871, 1248
BssNAI GTATAC 1 cut(s) 611
Bst1107I GTATAC 1 cut(s) 611
Bst2UI CCWGG 3 cut(s) 133, 503, 1230
Bst4CI ACNGT 5 cut(s) 161, 475, 540, 884, 1190
Bst6I CTCTTC 1 cut(s) 239
BstAPI GCANNNNNTGC 1 cut(s) 845
BstAUI TGTACA 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 129, 596, 700
BstDEI CTNAG 4 cut(s) 280, 895, 1135, 1262
BstKTI GATC 5 cut(s) 67, 495, 712, 874, 1251
BstMAI GTCTC 2 cut(s) 150, 1329
BstMBI GATC 5 cut(s) 64, 492, 709, 871, 1248
BstMWI GCNNNNNNNGC 5 cut(s) 86, 133, 179, 845, 896
BstNI CCWGG 3 cut(s) 133, 503, 1230
BstNSI RCATGY 4 cut(s) 515, 598, 702, 1057
BstSCI CCNGG 3 cut(s) 131, 501, 1228
BstSLI GKGCMC 1 cut(s) 519
BstV1I GCAGC 4 cut(s) 296, 849, 860, 928
BstXI CCANNNNNNTGG 2 cut(s) 358, 1312
BstZ17I GTATAC 1 cut(s) 611
BsuRI GGCC 2 cut(s) 258, 890
BtrI CACGTC 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 1376
BtsIMutI CAGTG 5 cut(s) 433, 889, 1128, 1195, 1376
Cac8I GCNNGC 3 cut(s) 129, 596, 700
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 1 cut(s) 1251
Cfr13I GGNCC 2 cut(s) 257, 888
Csp6I GTAC 2 cut(s) 103, 200
CviQI GTAC 2 cut(s) 103, 200
DdeI CTNAG 4 cut(s) 280, 895, 1135, 1262
DpnI GATC 5 cut(s) 66, 494, 711, 873, 1250
DpnII GATC 5 cut(s) 64, 492, 709, 871, 1248
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 2 cut(s) 257, 888
EcoRII CCWGG 3 cut(s) 131, 501, 1228
EcoRV GATATC 1 cut(s) 141
EcoT22I ATGCAT 2 cut(s) 700, 704
EcoT38I GRGCYC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 1 cut(s) 492
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 4 cut(s) 285, 849, 863, 942
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 4 cut(s) 285, 849, 863, 942
GluI GCNGC 4 cut(s) 285, 849, 863, 942
GsuI CTGGAG 1 cut(s) 1212
HaeIII GGCC 2 cut(s) 258, 890
HindIII AAGCTT 2 cut(s) 110, 1307
HinfI GANTC 4 cut(s) 146, 1003, 1009, 1133
HphI GGTGA 2 cut(s) 1166, 1343
Hpy166II GTNNAC 5 cut(s) 517, 611, 667, 797, 1051
Hpy188I TCNGA 3 cut(s) 64, 145, 1008
Hpy188III TCNNGA 6 cut(s) 726, 737, 875, 1124, 1252, 1382
Hpy8I GTNNAC 5 cut(s) 517, 611, 667, 797, 1051
Hpy99I CGWCG 1 cut(s) 1243
HpyAV CCTTC 2 cut(s) 948, 1159
HpyCH4III ACNGT 5 cut(s) 161, 475, 540, 884, 1190
HpyCH4IV ACGT 3 cut(s) 165, 663, 1241
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 133, 179, 845, 896
HpyF3I CTNAG 4 cut(s) 280, 895, 1135, 1262
HpySE526I ACGT 3 cut(s) 165, 663, 1241
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 5 cut(s) 64, 492, 709, 871, 1248
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 2 cut(s) 229, 639
Lsp1109I GCAGC 4 cut(s) 296, 849, 860, 928
LweI GCATC 4 cut(s) 211, 432, 517, 540
MaeII ACGT 3 cut(s) 165, 663, 1241
MaeIII GTNAC 3 cut(s) 55, 161, 555
MalI GATC 5 cut(s) 66, 494, 711, 873, 1250
MboI GATC 5 cut(s) 64, 492, 709, 871, 1248
MfeI CAATTG 1 cut(s) 27
MhlI GDGCHC 3 cut(s) 234, 519, 725
MluCI AATT 9 cut(s) 27, 336, 396, 448, 649, 672, 748, 1176, 1295
MlyI GAGTC 2 cut(s) 140, 1127
MmeI TCCRAC 1 cut(s) 980
MnlI CCTC 4 cut(s) 348, 651, 1057, 1239
Mph1103I ATGCAT 2 cut(s) 700, 704
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 6 cut(s) 293, 567, 653, 1104, 1179, 1359
MslI CAYNNNNRTG 4 cut(s) 242, 345, 825, 1188
MspA1I CMGCKG 1 cut(s) 851
MspR9I CCNGG 3 cut(s) 133, 503, 1230
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 2 cut(s) 370, 743
MvaI CCWGG 3 cut(s) 133, 503, 1230
MwoI GCNNNNNNNGC 5 cut(s) 86, 133, 179, 845, 896
NdeI CATATG 1 cut(s) 615
NdeII GATC 5 cut(s) 64, 492, 709, 871, 1248
NlaIV GGNNCC 2 cut(s) 259, 889
NmuCI GTSAC 3 cut(s) 55, 161, 555
NsiI ATGCAT 2 cut(s) 700, 704
NspI RCATGY 4 cut(s) 515, 598, 702, 1057
PaeI GCATGC 2 cut(s) 598, 702
PagI TCATGA 1 cut(s) 1251
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 239
PctI GAATGC 2 cut(s) 370, 743
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 1003, 1009
PkrI GCNGC 4 cut(s) 286, 850, 864, 943
PleI GAGTC 2 cut(s) 140, 1127
PpsI GAGTC 2 cut(s) 140, 1127
PscI ACATGT 1 cut(s) 511
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 131, 501, 1228
PspGI CCWGG 3 cut(s) 131, 501, 1228
PspN4I GGNNCC 2 cut(s) 259, 889
PspPI GGNCC 2 cut(s) 257, 888
PstNI CAGNNNCTG 1 cut(s) 380
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 2 cut(s) 104, 201
RsaNI GTAC 2 cut(s) 103, 200
RseI CAYNNNNRTG 4 cut(s) 242, 345, 825, 1188
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 6 cut(s) 293, 567, 653, 1104, 1179, 1359
SatI GCNGC 4 cut(s) 285, 849, 863, 942
Sau3AI GATC 5 cut(s) 64, 492, 709, 871, 1248
Sau96I GGNCC 2 cut(s) 257, 888
SchI GAGTC 2 cut(s) 140, 1127
ScrFI CCNGG 3 cut(s) 133, 503, 1230
SduI GDGCHC 3 cut(s) 234, 519, 725
SfaNI GCATC 4 cut(s) 211, 432, 517, 540
SmiMI CAYNNNNRTG 4 cut(s) 242, 345, 825, 1188
SphI GCATGC 2 cut(s) 598, 702
Sse9I AATT 9 cut(s) 27, 336, 396, 448, 649, 672, 748, 1176, 1295
SsiI CCGC 1 cut(s) 87
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 3 cut(s) 131, 501, 1228
TaaI ACNGT 5 cut(s) 161, 475, 540, 884, 1190
TaiI ACGT 3 cut(s) 168, 666, 1244
TaqI TCGA 1 cut(s) 305
TasI AATT 9 cut(s) 27, 336, 396, 448, 649, 672, 748, 1176, 1295
TatI WGTACW 1 cut(s) 199
TfiI GAWTC 2 cut(s) 1003, 1009
Tru1I TTAA 6 cut(s) 293, 567, 653, 1104, 1179, 1359
Tru9I TTAA 6 cut(s) 293, 567, 653, 1104, 1179, 1359
TscAI CASTG 5 cut(s) 433, 889, 1135, 1195, 1376
TseFI GTSAC 3 cut(s) 55, 161, 555
TseI GCWGC 4 cut(s) 284, 848, 862, 941
Tsp45I GTSAC 3 cut(s) 55, 161, 555
TspDTI ATGAA 6 cut(s) 226, 329, 809, 977, 1013, 1268
TspRI CASTG 5 cut(s) 433, 889, 1135, 1195, 1376
VneI GTGCAC 1 cut(s) 515
XapI RAATTY 2 cut(s) 336, 1295
XceI RCATGY 4 cut(s) 515, 598, 702, 1057
XmiI GTMKAC 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 443
Zsp2I ATGCAT 2 cut(s) 700, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.