MD16G1288300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012285
Physical Location & Seq
Forward (+)
41362142 .. 41363625
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1288300.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGCGGATTTCTCCTTCTACCTCTCCGATACCGACGAATCAGCTGTGGACGACCTCATCTCCCAAGCCCAAGACCTCTGCGTTCTCGAGCAAGTTTCCGCCATCAACTGCTCCGGCTTCACCGACTCTCTCCTCCCCACTGAACTTGAATCCCGCTTCCGCAAACTCAAATCCTTCCCAGAGAGCTCCGCCTCCCGCCCGCCACAGTTAACCACCGGTTCAGCTCGGCGCAATTCGGTTGAAGGTAAGACAGACCCTGTTTCTGATGCTGCCATTTTCTCGCCTTCCAAACAGAACCCAGATGAGAAATTGGGGTCAAATCTCAAATCTGATCCCTCAGAATTTTCAGTAGCGAAATCGAAATACAACCCAGGTGGGAAAAGGGGTTCAAAGGCGAAATTGAAAAGTGGTTCAGTCTCATCCCCTTCAAATTCCTCCAATTCTTCTCCGGAAAGTGCAATTTTCTCGCCATCCAAACAGAACCCAGATAAGAAGCAGCGTTCTACGCCCAAACCGAAATCTCGGTCGTTTGATTCTCCGCCGGATTCTTCTAATTCGTTGACGGACTCCCCTTCGCCCCCGAGGAAATCGGGTTGCTTGTGGTGTTCCCCGAAGACAAAGAGGGCTTCTCACACAAAGAGTAAAGAAAAAGGGGCAATTGACTTCGGGGCTGGTCTGAATTTCAGCGACGATGATGGGCTTTTATCGGATTTGGGCAGTTTTTCGAAGAAAGAGCAGGCGAGGAAGCTGAAGAAGGCGATGAAGGAGGAGGAGAAGATTTGCAGAGAGGCTGAGAAAATTGTCAAGTGGGCTAAGCAGGCGTCGGCAAGGATGAGTGCAGCTGCCATTGAAGATGAACTTGTGAGCCAGATATCCCATTGCACACAGGGAAATGATTTTTCGTTTCTGGGGCGTTGCCTTGTTGGATCTCCGGCGTCTGCAGGGAAGCTGGTTCGTGGTGGATGGTTGGTGCTCCGGCGGCGGCAGAGGAAACTGGCTGCTGGGGTTTGTTTTGTGTTTTTCCGTTTTGGGCTCTTGTTGCTATGGGGGTCCATGCCCTTGTTGCGTGCTTCAATTGTAAATGAAGTTAATGAACCTCCGTCTCTTTGTATAATTTGGATTCCATACATTCATGATTTGAATGTAAAGCAGTCGGATTACTTGTCCCTCGATCTATTAGTGAGCTATCTCTCCACTGATTGTGAAAGATGTTCCACTAGACGTCAACCTACTTTAGGTGATATTTTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

45.55

Weight (kDa)

9.12

Isoelectric Point (pI)

55.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23270 AT1G31460
fragaria_vesca FvH4_5g24380
malus_domestica MD16G1288300.v1.1
prunus_persica Prupe.5G017000_v2.0.a1
pyrus_communis pycom16g25600
rosa_chinensis RchiOBHm_Chr7g0214771
rosa_laevigata RLG00000002723 RLG00000020760
rosa_multiflora Rmu_co8342721.1_g000001 Rmu_sc0002308.1_g000045 Rmu_sc0026107.1_g000001
rosa_roxburghii Rroxscaffold_3G00245100
rosa_rugosa Rorug06G0112700
rosa_samantha Rh7AG283900 Rh7BG274500 Rh7CG303300 Rh7DG287200
rosa_wichuraiana Rw7G024200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1225
AccIII TCCGGA 1 cut(s) 448
AclWI GGATC 2 cut(s) 326, 934
AcsI RAATTY 3 cut(s) 341, 430, 679
AcuI CTGAAG 1 cut(s) 770
AcyI GRCGYC 3 cut(s) 821, 935, 1222
AfiI CCNNNNNNNGG 1 cut(s) 1235
AgeI ACCGGT 1 cut(s) 215
AgsI TTSAA 8 cut(s) 149, 242, 390, 403, 429, 851, 1074, 1141
AjnI CCWGG 1 cut(s) 370
AloI GAACNNNNNNTCC 2 cut(s) 370, 402
AluBI AGCT 7 cut(s) 44, 186, 224, 748, 842, 949, 1185
AluI AGCT 7 cut(s) 44, 186, 224, 748, 842, 949, 1185
Alw21I GWGCWC 2 cut(s) 188, 975
Alw26I GTCTC 2 cut(s) 421, 1107
AlwI GGATC 2 cut(s) 326, 934
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 2 cut(s) 86, 580
Aor13HI TCCGGA 1 cut(s) 448
ApeKI GCWGC 5 cut(s) 269, 496, 839, 842, 998
ApoI RAATTY 3 cut(s) 341, 430, 679
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AsiGI ACCGGT 1 cut(s) 215
AspLEI GCGC 1 cut(s) 231
AspS9I GGNCC 1 cut(s) 1050
AsuHPI GGTGA 2 cut(s) 112, 1250
AsuII TTCGAA 1 cut(s) 725
AvaI CYCGRG 2 cut(s) 86, 580
AvaII GGWCC 1 cut(s) 1050
BanII GRGCYC 2 cut(s) 188, 1035
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 2 cut(s) 188, 975
BbvI GCAGC 5 cut(s) 256, 508, 829, 851, 985
BccI CCATC 4 cut(s) 110, 478, 689, 957
BcgI CGANNNNNNTGC 2 cut(s) 447, 481
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 421, 1107
BfaI CTAG 1 cut(s) 1218
BfmI CTRYAG 1 cut(s) 939
BisI GCNGC 7 cut(s) 270, 497, 840, 843, 980, 983, 999
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 7 cut(s) 271, 498, 841, 844, 981, 984, 1000
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 1 cut(s) 1050
BmeT110I CYCGRG 2 cut(s) 86, 580
BmgT120I GGNCC 1 cut(s) 1050
BmiI GGNNCC 1 cut(s) 1051
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 256
BpiI GAAGAC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 613, 645
Bpu1102I GCTNAGC 1 cut(s) 813
Bpu14I TTCGAA 1 cut(s) 725
BsaHI GRCGYC 3 cut(s) 821, 935, 1222
BsaJI CCNNGG 2 cut(s) 370, 581
BsaWI WCCGGW 2 cut(s) 215, 448
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 1235
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 999
Bse3DI GCAATG 1 cut(s) 877
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 370, 581
BseGI GGATG 4 cut(s) 419, 470, 837, 968
BseLI CCNNNNNNNGG 1 cut(s) 1235
BseMI GCAATG 1 cut(s) 877
BseMII CTCAG 2 cut(s) 351, 783
BseNI ACTGG 1 cut(s) 999
BseRI GAGGAG 3 cut(s) 122, 782, 785
BseXI GCAGC 5 cut(s) 256, 508, 829, 851, 985
BseYI CCCAGC 1 cut(s) 1001
BsgI GTGCAG 1 cut(s) 858
Bsh1285I CGRYCG 1 cut(s) 527
BshTI ACCGGT 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 2 cut(s) 188, 975
BsiHKCI CYCGRG 2 cut(s) 86, 580
BsiSI CCGG 6 cut(s) 114, 216, 449, 542, 932, 976
BslFI GGGAC 1 cut(s) 1150
BslI CCNNNNNNNGG 1 cut(s) 1235
BsmAI GTCTC 2 cut(s) 421, 1107
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 1 cut(s) 1150
BsoBI CYCGRG 2 cut(s) 86, 580
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 3 cut(s) 188, 975, 1035
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 331, 926, 1171
Bsp1720I GCTNAGC 1 cut(s) 813
BspCNI CTCAG 2 cut(s) 350, 784
BspEI TCCGGA 1 cut(s) 448
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 1 cut(s) 1051
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 2 cut(s) 326, 934
BspT104I TTCGAA 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 1 cut(s) 999
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 370, 581
BssMI GATC 3 cut(s) 331, 926, 1171
BssNI GRCGYC 3 cut(s) 821, 935, 1222
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 207
BstACI GRCGYC 3 cut(s) 821, 935, 1222
BstBI TTCGAA 1 cut(s) 725
BstC8I GCNNGC 4 cut(s) 200, 738, 819, 1068
BstDEI CTNAG 3 cut(s) 337, 792, 813
BstENI CCTNNNNNAGG 1 cut(s) 1233
BstF5I GGATG 4 cut(s) 419, 470, 837, 968
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 3 cut(s) 334, 929, 1174
BstMAI GTCTC 2 cut(s) 421, 1107
BstMBI GATC 3 cut(s) 331, 926, 1171
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 5 cut(s) 505, 818, 979, 1039, 1063
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 1 cut(s) 939
BstV1I GCAGC 5 cut(s) 256, 508, 829, 851, 985
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
BtgZI GCGATG 1 cut(s) 773
BtsCI GGATG 4 cut(s) 419, 470, 837, 968
BtsIMutI CAGTG 2 cut(s) 138, 1194
Cac8I GCNNGC 4 cut(s) 200, 738, 819, 1068
CaiI CAGNNNCTG 1 cut(s) 257
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 1 cut(s) 231
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 1050
CseI GACGC 2 cut(s) 810, 924
CspAI ACCGGT 1 cut(s) 215
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 355, 390
CviAII CATG 2 cut(s) 1054, 1133
DdeI CTNAG 3 cut(s) 337, 792, 813
DpnI GATC 3 cut(s) 333, 928, 1173
DpnII GATC 3 cut(s) 331, 926, 1171
EciI GGCGGA 4 cut(s) 20, 88, 178, 528
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 2 cut(s) 188, 1035
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 1 cut(s) 1050
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 2 cut(s) 86, 580
EcoICRI GAGCTC 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 1233
EcoRII CCWGG 1 cut(s) 370
EcoRV GATATC 1 cut(s) 873
EcoT38I GRGCYC 2 cut(s) 188, 1035
Esp3I CGTCTC 1 cut(s) 1107
FaeI CATG 2 cut(s) 1057, 1136
FaiI YATR 5 cut(s) 1045, 1055, 1112, 1126, 1134
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FaqI GGGAC 1 cut(s) 1150
FatI CATG 2 cut(s) 1053, 1132
FauI CCCGC 3 cut(s) 161, 203, 207
Fnu4HI GCNGC 7 cut(s) 270, 497, 840, 843, 980, 983, 999
FokI GGATG 4 cut(s) 406, 457, 844, 975
FriOI GRGCYC 2 cut(s) 188, 1035
Fsp4HI GCNGC 7 cut(s) 270, 497, 840, 843, 980, 983, 999
FspBI CTAG 1 cut(s) 1218
GlaI GCGC 1 cut(s) 230
GluI GCNGC 7 cut(s) 270, 497, 840, 843, 980, 983, 999
GsaI CCCAGC 1 cut(s) 1005
HapII CCGG 6 cut(s) 114, 216, 449, 542, 932, 976
HgaI GACGC 2 cut(s) 810, 924
HhaI GCGC 1 cut(s) 231
Hin1I GRCGYC 3 cut(s) 821, 935, 1222
Hin1II CATG 2 cut(s) 1057, 1136
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HincII GTYRAC 3 cut(s) 210, 561, 1226
HindII GTYRAC 3 cut(s) 210, 561, 1226
HinfI GANTC 7 cut(s) 38, 125, 149, 533, 545, 566, 1120
HpaI GTTAAC 1 cut(s) 210
HpaII CCGG 6 cut(s) 114, 216, 449, 542, 932, 976
HphI GGTGA 2 cut(s) 112, 1250
Hpy166II GTNNAC 4 cut(s) 49, 210, 561, 1226
Hpy188I TCNGA 7 cut(s) 28, 265, 331, 340, 678, 709, 1156
Hpy188III TCNNGA 3 cut(s) 86, 449, 1133
Hpy8I GTNNAC 4 cut(s) 49, 210, 561, 1226
Hpy99I CGWCG 3 cut(s) 38, 692, 826
HpyAV CCTTC 8 cut(s) 25, 184, 236, 294, 435, 582, 748, 757
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 1222
HpyCH4V TGCA 5 cut(s) 458, 783, 839, 882, 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 505, 818, 979, 1039, 1063
HpyF3I CTNAG 3 cut(s) 337, 792, 813
HpySE526I ACGT 1 cut(s) 1222
Hsp92I GRCGYC 3 cut(s) 821, 935, 1222
Hsp92II CATG 2 cut(s) 1057, 1136
HspAI GCGC 1 cut(s) 229
Kpn2I TCCGGA 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 210
Kzo9I GATC 3 cut(s) 331, 926, 1171
LmnI GCTCC 3 cut(s) 116, 191, 978
Lsp1109I GCAGC 5 cut(s) 256, 508, 829, 851, 985
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 1218
MaeII ACGT 1 cut(s) 1222
MalI GATC 3 cut(s) 333, 928, 1173
MboI GATC 3 cut(s) 331, 926, 1171
MboII GAAGA 7 cut(s) 435, 540, 625, 739, 763, 787, 863
MfeI CAATTG 2 cut(s) 657, 1074
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 3 cut(s) 188, 975, 1035
MlyI GAGTC 2 cut(s) 119, 560
MmeI TCCRAC 2 cut(s) 904, 1134
MroI TCCGGA 1 cut(s) 448
MseI TTAA 2 cut(s) 209, 1089
MspA1I CMGCKG 2 cut(s) 44, 842
MspI CCGG 6 cut(s) 114, 216, 449, 542, 932, 976
MspR9I CCNGG 1 cut(s) 372
MunI CAATTG 2 cut(s) 657, 1074
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 5 cut(s) 505, 818, 979, 1039, 1063
NdeII GATC 3 cut(s) 331, 926, 1171
NlaIII CATG 2 cut(s) 1057, 1136
NlaIV GGNNCC 1 cut(s) 1051
NmeAIII GCCGAG 1 cut(s) 205
NspV TTCGAA 1 cut(s) 725
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 1132
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PfeI GAWTC 5 cut(s) 38, 149, 533, 545, 1120
PinAI ACCGGT 1 cut(s) 215
PkrI GCNGC 7 cut(s) 271, 498, 841, 844, 981, 984, 1000
PleI GAGTC 2 cut(s) 119, 560
PpsI GAGTC 2 cut(s) 119, 560
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 370
PspFI CCCAGC 1 cut(s) 1001
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 1051
PspPI GGNCC 1 cut(s) 1050
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 926
PvuII CAGCTG 2 cut(s) 44, 842
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 209, 1089
SatI GCNGC 7 cut(s) 270, 497, 840, 843, 980, 983, 999
Sau3AI GATC 3 cut(s) 331, 926, 1171
Sau96I GGNCC 1 cut(s) 1050
SchI GAGTC 2 cut(s) 119, 560
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 3 cut(s) 188, 975, 1035
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 939
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 725
SinI GGWCC 1 cut(s) 1050
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SspMI CTAG 1 cut(s) 1218
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 1 cut(s) 1225
TaqI TCGA 4 cut(s) 87, 359, 725, 1170
TaqII GACCGA 1 cut(s) 513
TauI GCSGC 2 cut(s) 982, 985
TfiI GAWTC 5 cut(s) 38, 149, 533, 545, 1120
Tru1I TTAA 2 cut(s) 209, 1089
Tru9I TTAA 2 cut(s) 209, 1089
TscAI CASTG 2 cut(s) 145, 1201
TseI GCWGC 5 cut(s) 269, 496, 839, 842, 998
TspDTI ATGAA 5 cut(s) 776, 870, 1098, 1107, 1121
TspGWI ACGGA 3 cut(s) 578, 1013, 1089
TspRI CASTG 2 cut(s) 145, 1201
VpaK11BI GGWCC 1 cut(s) 1050
XagI CCTNNNNNAGG 1 cut(s) 1233
XapI RAATTY 3 cut(s) 341, 430, 679
XhoI CTCGAG 1 cut(s) 86
XspI CTAG 1 cut(s) 1218
ZraI GACGTC 1 cut(s) 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.