MD17G1039100.v1.1
ERF Family

heavy metal transport detoxification superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013272
Physical Location & Seq
Forward (+)
2856401 .. 2858336
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1039100.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCCACCGTTTCAGCCTCCACATTCTCTCCTATCCCATCCTTCCGCACTCCAAACCATTCCCATTCCCTCGGGATTCCGATTCTCTCTCCAACCGTCTCGTTTCGGAACGTTTCCGCCTCTTTCCAGCGGTTGACGCACAAGGACCGCCGGTTCTGGAGACCCACAATTCTGAAGGCCGTCGAAGAGGAAACCCCAATTCCAGCTGAAGAAGAAGCAGCGGTCAGCAGCGAGCAGCCGGAGCAGCCCGTTTCGGTCCCCGTTTCGCCCTCCGATACTCTCACCATGGTTTTCCAGGCAGATGGAACGATGAGTGATGCGGCTATCCCATCAGTAACCAAAGCCTTACAGGAAACTGAAGGCATTACCAACTTAAAAGTTGAAGTCATTGAGGGTATTGCAACTGTTGAGTTGACGAAACAGACGACTGTACAAGCTACCGGGGTGGCTTCCAGTTTGGTGGAGACCATACAGAGTAAAGGATTTAAGCTACAAACATTGAATTTAAGCTTTGATGAGGAAGAGGAAGTTGCTGCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.4

Weight (kDa)

4.73

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14910 AT5G14910
fragaria_vesca FvH4_6g50270
malus_domestica MD17G1039100.v1.1
prunus_persica Prupe.3G279900_v2.0.a1
pyrus_communis pycom17g03530
rosa_chinensis RchiOBHm_Chr2g0170541 RchiOBHm_Chr2g0170581
rosa_laevigata RLG00000021965
rosa_multiflora Rmu_sc0003787.1_g000037
rosa_roxburghii Rroxscaffold_2G00081210
rosa_rugosa Rorug02G0549700 Rorug02G0549700
rosa_samantha Rh2AG621600 Rh2BG633000 Rh2CG602300 Rh2DG645800
rosa_wichuraiana Rw2G051540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 46, 117, 130, 148, 221, 320
AclI AACGTT 1 cut(s) 111
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 3 cut(s) 194, 228, 378
AfaI GTAC 1 cut(s) 432
AgsI TTSAA 2 cut(s) 383, 502
AjnI CCWGG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 206, 437, 490, 510
AluI AGCT 4 cut(s) 206, 437, 490, 510
Alw26I GTCTC 3 cut(s) 103, 154, 458
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 2 cut(s) 3, 177
ApeKI GCWGC 5 cut(s) 218, 228, 235, 244, 533
ApoI RAATTY 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 145, 256
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 2 cut(s) 145, 256
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 5 cut(s) 230, 240, 247, 256, 520
BccI CCATC 3 cut(s) 46, 296, 337
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 103, 154, 458
BisI GCNGC 6 cut(s) 219, 229, 236, 245, 321, 534
BlsI GCNGC 6 cut(s) 220, 230, 237, 246, 322, 535
Bme1390I CCNGG 2 cut(s) 296, 442
Bme18I GGWCC 2 cut(s) 145, 256
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 145, 256
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 296, 442
BmsI GCATC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 442
BsaI GGTCTC 2 cut(s) 154, 458
BsaJI CCNNGG 3 cut(s) 70, 285, 441
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 70, 285, 441
BseGI GGATG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 453
BseXI GCAGC 5 cut(s) 230, 240, 247, 256, 520
BshFI GGCC 2 cut(s) 5, 179
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 3 cut(s) 151, 239, 441
BslFI GGGAC 1 cut(s) 242
BsmAI GTCTC 3 cut(s) 103, 154, 458
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 2 cut(s) 5, 179
Bso31I GGTCTC 2 cut(s) 154, 458
BsoBI CYCGRG 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 430
Bsp19I CCATGG 1 cut(s) 285
BspACI CCGC 6 cut(s) 46, 117, 130, 148, 221, 320
BspANI GGCC 2 cut(s) 5, 179
BspLI GGNNCC 1 cut(s) 258
BspTNI GGTCTC 2 cut(s) 154, 458
BsrFI RCCGGY 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 1 cut(s) 453
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 70, 285, 441
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 10, 97, 406, 430
Bst6I CTCTTC 2 cut(s) 180, 516
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 1 cut(s) 233
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 38
BstMAI GTCTC 3 cut(s) 103, 154, 458
BstMWI GCNNNNNNNGC 3 cut(s) 136, 241, 244
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 2 cut(s) 294, 440
BstV1I GCAGC 5 cut(s) 230, 240, 247, 256, 520
BstXI CCANNNNNNTGG 2 cut(s) 302, 460
BsuRI GGCC 2 cut(s) 5, 179
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 145, 256
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 431
CviAII CATG 1 cut(s) 286
CviQI GTAC 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 180, 516
EarI CTCTTC 2 cut(s) 180, 516
EciI GGCGGA 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 285
Eco31I GGTCTC 2 cut(s) 154, 458
Eco47I GGWCC 2 cut(s) 145, 256
Eco57I CTGAAG 3 cut(s) 194, 228, 378
Eco88I CYCGRG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 1 cut(s) 289
FaiI YATR 2 cut(s) 287, 470
FaqI GGGAC 1 cut(s) 242
FatI CATG 1 cut(s) 285
Fnu4HI GCNGC 6 cut(s) 219, 229, 236, 245, 321, 534
FokI GGATG 1 cut(s) 25
Fsp4HI GCNGC 6 cut(s) 219, 229, 236, 245, 321, 534
GluI GCNGC 6 cut(s) 219, 229, 236, 245, 321, 534
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 2 cut(s) 5, 179
HapII CCGG 3 cut(s) 151, 239, 441
HgaI GACGC 1 cut(s) 145
Hin1II CATG 1 cut(s) 289
HincII GTYRAC 2 cut(s) 135, 414
HindII GTYRAC 2 cut(s) 135, 414
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 2 cut(s) 76, 82
HpaII CCGG 3 cut(s) 151, 239, 441
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 135, 414
Hpy188I TCNGA 5 cut(s) 81, 108, 174, 274, 542
Hpy188III TCNNGA 2 cut(s) 73, 157
Hpy8I GTNNAC 2 cut(s) 135, 414
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 3 cut(s) 52, 169, 353
HpyCH4III ACNGT 4 cut(s) 10, 97, 406, 430
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 1 cut(s) 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 241, 244
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 1 cut(s) 289
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 5 cut(s) 230, 240, 247, 256, 520
LweI GCATC 1 cut(s) 307
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 334
MboII GAAGA 4 cut(s) 197, 221, 224, 533
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 168, 198, 502
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 8 cut(s) 28, 80, 130, 181, 280, 385, 511, 517
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 374, 486, 506
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 130, 206, 221
MspI CCGG 3 cut(s) 151, 239, 441
MspR9I CCNGG 2 cut(s) 296, 442
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 136, 241, 244
NciI CCSGG 1 cut(s) 442
NcoI CCATGG 1 cut(s) 285
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PfeI GAWTC 2 cut(s) 76, 82
PkrI GCNGC 6 cut(s) 220, 230, 237, 246, 322, 535
Psp1406I AACGTT 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 2 cut(s) 145, 256
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SaqAI TTAA 3 cut(s) 374, 486, 506
SatI GCNGC 6 cut(s) 219, 229, 236, 245, 321, 534
Sau96I GGNCC 2 cut(s) 145, 256
ScrFI CCNGG 2 cut(s) 296, 442
SetI ASST 5 cut(s) 114, 208, 439, 492, 512
SfaNI GCATC 1 cut(s) 307
SinI GGWCC 2 cut(s) 145, 256
Sse9I AATT 3 cut(s) 168, 198, 502
SsiI CCGC 6 cut(s) 46, 117, 130, 148, 221, 320
StyD4I CCNGG 2 cut(s) 294, 440
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 4 cut(s) 10, 97, 406, 430
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 183
TaqII GACCGA 1 cut(s) 244
TasI AATT 3 cut(s) 168, 198, 502
TatI WGTACW 1 cut(s) 430
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 2 cut(s) 76, 82
Tru1I TTAA 3 cut(s) 374, 486, 506
Tru9I TTAA 3 cut(s) 374, 486, 506
TseI GCWGC 5 cut(s) 218, 228, 235, 244, 533
VpaK11BI GGWCC 2 cut(s) 145, 256
XapI RAATTY 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.