MD17G1100200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006274
Physical Location & Seq
Reverse (-)
8515435 .. 8517453
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1100200.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGGGTAGCCAAAACCTTGAGTTCCACGTCTTCTTGTTCCCTTACATGGGTCGCGGCCACATACCGATCTCAGATATGGCCAAGCTATTCGCAGCGCAAGGAGCGAGGACAACCATAGTCACAACTCCTCTCAACGCTTCAACATTCTCCAAAGCCACTCAGTCAAGCAAAACCAATTCCGGCAGCGTCAAAGTCGAAATCAAAACCATCAAGTTCCCTTCTGTAGAAGCCGGTCTGCCAGATGGTTGTGAGAATCTCGACTCACTCCCCCCACCAGATGGTTGTGGACATCGTGATTTGGATCCATCCCCGCCGTACCTGAGGTGCAGGCATTGCAAAGAGCGGCTGCTGAGGGGCGTTCAGACGCTGACCTGCGTCTTCTGCGGCAGAGAACACTGCAAGGACCTCCCTCCCGACCCCATCAATTTCCGGGATACTTTTGGGTACCGTTGGCTGCTCCAGTACCTGAGCTTAAGTGGATCGGAGATAGTGGATTTACCTGTTGGAGCAAATGAATTAAACAGAACACAGACTGCGCGGAAGGATGAGTTAAGTCTGGCTGATCTCTTAAATCTTGAAATAAAATGGACCTCTAAGCCAGAGAAAATTGAGACTGATCTCCCAAATGAGATCCCTAATCTGCTCAAATTACCAGATTTGGCTGGAGTCAATCTCGACAACTTTTTCTTAGAAGGAAAGAAAGGTGGTGCTTTGAGTAATTCCGAAGAGTTGTTCAGGTCTAGCACACAGATTGTCGGTAAAGAGCGTTTCCAAGGTCATGAAACTCTTAGGTTGTTTGAGAATGTAAAGCCTTTTGAGACAGTTGTGCAGACTACTGAAGGAGAGAGTGGTGATTCTGGTTGGGCGGCAAGTTTTCAGTTTGCTGCTTCTGAATCACAAGCTTCTGGAAACCTTCCTCAAGCTTCTGAAACCCTTCCTCCGGCCTCTGGAAACCTTCCTCAAGCTTCTGAAATCCTTCCTCGAGCTTCTGAAAACCTCCCTCAAGCTTCTGAAAGCTTTCCCCAAGAATCAAAATCCCTTGACCGCTTTGTGGGTTCCACGGTGGATCTTTCTGCCTACATAGGCACAGTATTTGGATCTGTAGTGGACTCAATGAATGAAAAATCAAACCATAGTGTGACTGGGTCTGCATCCATGTCCTCCAACTGGTTTCAAGATGATCTGTTGGGCGTTTCCAACTCTGGGTTTCTTGAGGGCCTGAACGGTTGGAAATCCTTGCTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

416

Amino Acids

45.28

Weight (kDa)

5.13

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7815 PF25122 105 - 131 2.1e-13 Domain of unknown function (DUF7815)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
Acc65I GGTACC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 343
AccII CGCG 2 cut(s) 54, 538
AciI CCGC 7 cut(s) 54, 311, 343, 384, 538, 866, 1045
AclWI GGATC 6 cut(s) 296, 309, 487, 625, 1074, 1105
AcoI YGGCCR 2 cut(s) 55, 78
AcuI CTGAAG 1 cut(s) 858
AfaI GTAC 3 cut(s) 317, 446, 464
AfiI CCNNNNNNNGG 9 cut(s) 46, 47, 278, 940, 947, 1051, 1167, 1203, 1242
AflII CTTAAG 1 cut(s) 472
AgsI TTSAA 3 cut(s) 141, 578, 1175
AjiI CACGTC 1 cut(s) 28
AluBI AGCT 8 cut(s) 85, 471, 902, 923, 965, 986, 1007, 1017
AluI AGCT 8 cut(s) 85, 471, 902, 923, 965, 986, 1007, 1017
Alw26I GTCTC 2 cut(s) 605, 812
AlwI GGATC 6 cut(s) 296, 309, 487, 625, 1074, 1105
AlwNI CAGNNNCTG 2 cut(s) 367, 466
Ama87I CYCGRG 1 cut(s) 981
AoxI GGCC 4 cut(s) 55, 78, 942, 1216
ApeKI GCWGC 5 cut(s) 92, 183, 346, 454, 884
Asp700I GAANNNNTTC 3 cut(s) 933, 975, 1017
Asp718I GGTACC 1 cut(s) 444
AspLEI GCGC 2 cut(s) 97, 538
AspS9I GGNCC 3 cut(s) 403, 588, 1216
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 981
AvaII GGWCC 2 cut(s) 403, 588
AxyI CCTNAGG 1 cut(s) 320
BalI TGGCCA 1 cut(s) 80
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 444
BbsI GAAGAC 2 cut(s) 22, 370
BbvCI CCTCAGC 2 cut(s) 350, 1241
BbvI GCAGC 5 cut(s) 104, 195, 333, 441, 871
BccI CCATC 5 cut(s) 215, 236, 272, 313, 428
BceAI ACGGC 1 cut(s) 298
BciVI GTATCC 1 cut(s) 427
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 605, 812
BfaI CTAG 1 cut(s) 741
BfmI CTRYAG 2 cut(s) 222, 1101
BfrI CTTAAG 1 cut(s) 472
BfuAI ACCTGC 1 cut(s) 380
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 9 cut(s) 55, 93, 184, 344, 347, 385, 455, 867, 885
BlsI GCNGC 9 cut(s) 56, 94, 185, 345, 348, 386, 456, 868, 886
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 2 cut(s) 403, 588
BmeT110I CYCGRG 1 cut(s) 981
BmgBI CACGTC 1 cut(s) 28
BmgT120I GGNCC 3 cut(s) 403, 588, 1216
BmiI GGNNCC 3 cut(s) 303, 446, 1057
BmrFI CCNGG 1 cut(s) 431
BmrI ACTGGG 1 cut(s) 1152
BmsI GCATC 1 cut(s) 1160
BmuI ACTGGG 1 cut(s) 1152
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 22, 370
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 2 cut(s) 443, 684
Bpu10I CCTNAGC 3 cut(s) 350, 467, 1241
BpuEI CTTGAG 5 cut(s) 38, 903, 945, 987, 1232
BpuMI CCSGG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 772, 1059
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 9 cut(s) 46, 47, 278, 940, 947, 1051, 1167, 1203, 1242
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 3 cut(s) 460, 1147, 1172
Bse21I CCTNAGG 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 772, 1059
BseGI GGATG 3 cut(s) 305, 550, 1151
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 9 cut(s) 46, 47, 278, 940, 947, 1051, 1167, 1203, 1242
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 6 cut(s) 84, 173, 311, 341, 458, 1232
BseNI ACTGG 3 cut(s) 460, 1147, 1172
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 5 cut(s) 104, 195, 333, 441, 871
BsgI GTGCAG 2 cut(s) 346, 848
Bsh1236I CGCG 2 cut(s) 54, 538
BshFI GGCC 4 cut(s) 57, 80, 944, 1218
BshNI GGYRCC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 981
BsiSI CCGG 4 cut(s) 180, 231, 430, 941
BslI CCNNNNNNNGG 9 cut(s) 46, 47, 278, 940, 947, 1051, 1167, 1203, 1242
BsmAI GTCTC 2 cut(s) 605, 812
BsnI GGCC 4 cut(s) 57, 80, 944, 1218
BsoBI CYCGRG 1 cut(s) 981
Bsp143I GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
BspACI CCGC 7 cut(s) 54, 311, 343, 384, 538, 866, 1045
BspANI GGCC 4 cut(s) 57, 80, 944, 1218
BspCNI CTCAG 6 cut(s) 83, 172, 312, 342, 459, 1233
BspFNI CGCG 2 cut(s) 54, 538
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 3 cut(s) 303, 446, 1057
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 6 cut(s) 296, 309, 487, 625, 1074, 1105
BspT107I GGYRCC 1 cut(s) 444
BspTI CTTAAG 1 cut(s) 472
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 3 cut(s) 460, 1147, 1172
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 772, 1059
BssMI GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
BssT1I CCWWGG 1 cut(s) 772
Bst4CI ACNGT 5 cut(s) 449, 823, 1063, 1090, 1226
Bst6I CTCTTC 1 cut(s) 720
BstAFI CTTAAG 1 cut(s) 472
BstAPI GCANNNNNTGC 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 9 cut(s) 70, 159, 320, 350, 467, 594, 688, 788, 1241
BstDSI CCRYGG 1 cut(s) 1059
BstENI CCTNNNNNAGG 1 cut(s) 1240
BstF5I GGATG 3 cut(s) 305, 550, 1151
BstFNI CGCG 2 cut(s) 54, 538
BstHHI GCGC 2 cut(s) 97, 538
BstKTI GATC 9 cut(s) 69, 304, 482, 565, 619, 633, 1069, 1100, 1183
BstMAI GTCTC 2 cut(s) 605, 812
BstMBI GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
BstMWI GCNNNNNNNGC 3 cut(s) 101, 333, 381
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 2 cut(s) 222, 1101
BstUI CGCG 2 cut(s) 54, 538
BstV1I GCAGC 5 cut(s) 104, 195, 333, 441, 871
BstV2I GAAGAC 2 cut(s) 22, 370
BstX2I RGATCY 4 cut(s) 301, 630, 1066, 1097
BstYI RGATCY 4 cut(s) 301, 630, 1066, 1097
Bsu36I CCTNAGG 1 cut(s) 320
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 4 cut(s) 57, 80, 944, 1218
BtgI CCRYGG 1 cut(s) 1059
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 3 cut(s) 305, 550, 1151
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 329
CaiI CAGNNNCTG 2 cut(s) 367, 466
CciI TCATGA 1 cut(s) 778
CfoI GCGC 2 cut(s) 97, 538
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 403, 588, 1216
CseI GACGC 3 cut(s) 175, 364, 373
Csp6I GTAC 3 cut(s) 316, 445, 463
CviAII CATG 3 cut(s) 46, 779, 1156
CviQI GTAC 3 cut(s) 316, 445, 463
DdeI CTNAG 9 cut(s) 70, 159, 320, 350, 467, 594, 688, 788, 1241
DpnI GATC 9 cut(s) 68, 303, 481, 564, 618, 632, 1068, 1099, 1182
DpnII GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
EaeI YGGCCR 2 cut(s) 55, 78
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco130I CCWWGG 1 cut(s) 772
Eco47I GGWCC 2 cut(s) 403, 588
Eco57I CTGAAG 1 cut(s) 858
Eco81I CCTNAGG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 981
EcoNI CCTNNNNNAGG 1 cut(s) 1240
EcoO109I RGGNCCY 2 cut(s) 403, 1216
EcoT14I CCWWGG 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 3 cut(s) 49, 782, 1159
FaiI YATR 8 cut(s) 47, 62, 77, 116, 780, 1082, 1134, 1157
FatI CATG 3 cut(s) 45, 778, 1155
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 9 cut(s) 55, 93, 184, 344, 347, 385, 455, 867, 885
FokI GGATG 3 cut(s) 292, 557, 1138
Fsp4HI GCNGC 9 cut(s) 55, 93, 184, 344, 347, 385, 455, 867, 885
FspBI CTAG 1 cut(s) 741
GlaI GCGC 2 cut(s) 96, 537
GluI GCNGC 9 cut(s) 55, 93, 184, 344, 347, 385, 455, 867, 885
GsuI CTGGAG 2 cut(s) 443, 684
HaeIII GGCC 4 cut(s) 57, 80, 944, 1218
HapII CCGG 4 cut(s) 180, 231, 430, 941
HgaI GACGC 3 cut(s) 175, 364, 373
HhaI GCGC 2 cut(s) 97, 538
Hin1II CATG 3 cut(s) 49, 782, 1159
Hin6I GCGC 2 cut(s) 95, 536
HinP1I GCGC 2 cut(s) 95, 536
HindIII AAGCTT 5 cut(s) 900, 921, 963, 1005, 1015
HinfI GANTC 7 cut(s) 253, 260, 666, 854, 893, 1028, 1109
HpaII CCGG 4 cut(s) 180, 231, 430, 941
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 2 cut(s) 287, 1108
Hpy188I TCNGA 9 cut(s) 73, 363, 484, 724, 892, 928, 970, 991, 1012
Hpy8I GTNNAC 2 cut(s) 287, 1108
HpyAV CCTTC 8 cut(s) 228, 535, 686, 833, 923, 944, 965, 986
HpyCH4III ACNGT 5 cut(s) 449, 823, 1063, 1090, 1226
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 5 cut(s) 327, 336, 399, 829, 1151
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 333, 381
HpyF3I CTNAG 9 cut(s) 70, 159, 320, 350, 467, 594, 688, 788, 1241
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 3 cut(s) 49, 782, 1159
HspAI GCGC 2 cut(s) 95, 536
KpnI GGTACC 1 cut(s) 448
Kzo9I GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
LmnI GCTCC 3 cut(s) 101, 462, 506
Lsp1109I GCAGC 5 cut(s) 104, 195, 333, 441, 871
LweI GCATC 1 cut(s) 1160
MaeI CTAG 1 cut(s) 741
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 118, 1138
MalI GATC 9 cut(s) 68, 303, 481, 564, 618, 632, 1068, 1099, 1182
MbiI CCGCTC 1 cut(s) 343
MboI GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
MboII GAAGA 3 cut(s) 22, 370, 737
MflI RGATCY 4 cut(s) 301, 630, 1066, 1097
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 6 cut(s) 175, 424, 515, 606, 647, 718
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 3 cut(s) 254, 675, 1103
MmeI TCCRAC 4 cut(s) 484, 1188, 1208, 1221
Mox20I TGGCCA 1 cut(s) 80
MroXI GAANNNNTTC 3 cut(s) 933, 975, 1017
MscI TGGCCA 1 cut(s) 80
MseI TTAA 4 cut(s) 473, 518, 551, 569
Msp20I TGGCCA 1 cut(s) 80
MspCI CTTAAG 1 cut(s) 472
MspI CCGG 4 cut(s) 180, 231, 430, 941
MspR9I CCNGG 1 cut(s) 431
MvnI CGCG 2 cut(s) 54, 538
MwoI GCNNNNNNNGC 3 cut(s) 101, 333, 381
NciI CCSGG 1 cut(s) 431
NdeII GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
NlaIII CATG 3 cut(s) 49, 782, 1159
NlaIV GGNNCC 3 cut(s) 303, 446, 1057
NmuCI GTSAC 2 cut(s) 118, 1138
PaeR7I CTCGAG 1 cut(s) 981
PagI TCATGA 1 cut(s) 778
PdmI GAANNNNTTC 3 cut(s) 933, 975, 1017
PfeI GAWTC 4 cut(s) 253, 854, 893, 1028
PflFI GACNNNGTC 1 cut(s) 1144
PflMI CCANNNNNTGG 1 cut(s) 278
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 9 cut(s) 56, 94, 185, 345, 348, 386, 456, 868, 886
PleI GAGTC 3 cut(s) 254, 674, 1103
PpsI GAGTC 3 cut(s) 254, 674, 1103
PpuMI RGGWCCY 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 403
PspN4I GGNNCC 3 cut(s) 303, 446, 1057
PspPI GGNCC 3 cut(s) 403, 588, 1216
PspPPI RGGWCCY 1 cut(s) 403
PspXI VCTCGAGB 1 cut(s) 981
PstNI CAGNNNCTG 2 cut(s) 367, 466
PsuI RGATCY 4 cut(s) 301, 630, 1066, 1097
PsyI GACNNNGTC 1 cut(s) 1144
RsaI GTAC 3 cut(s) 317, 446, 464
RsaNI GTAC 3 cut(s) 316, 445, 463
SaqAI TTAA 4 cut(s) 473, 518, 551, 569
SatI GCNGC 9 cut(s) 55, 93, 184, 344, 347, 385, 455, 867, 885
Sau3AI GATC 9 cut(s) 66, 301, 479, 562, 616, 630, 1066, 1097, 1180
Sau96I GGNCC 3 cut(s) 403, 588, 1216
SchI GAGTC 3 cut(s) 254, 675, 1103
ScrFI CCNGG 1 cut(s) 431
SfaNI GCATC 1 cut(s) 1160
SfcI CTRYAG 2 cut(s) 222, 1101
Sfr274I CTCGAG 1 cut(s) 981
SinI GGWCC 2 cut(s) 403, 588
SlaI CTCGAG 1 cut(s) 981
SmlI CTYRAG 7 cut(s) 17, 472, 918, 960, 981, 1002, 1211
SmoI CTYRAG 7 cut(s) 17, 472, 918, 960, 981, 1002, 1211
Sse9I AATT 6 cut(s) 175, 424, 515, 606, 647, 718
SsiI CCGC 7 cut(s) 54, 311, 343, 384, 538, 866, 1045
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 5 cut(s) 449, 823, 1063, 1090, 1226
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 195, 258, 675, 982
TasI AATT 6 cut(s) 175, 424, 515, 606, 647, 718
TauI GCSGC 4 cut(s) 57, 346, 387, 869
TfiI GAWTC 4 cut(s) 253, 854, 893, 1028
Tru1I TTAA 4 cut(s) 473, 518, 551, 569
Tru9I TTAA 4 cut(s) 473, 518, 551, 569
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 2 cut(s) 118, 1138
TseI GCWGC 5 cut(s) 92, 183, 346, 454, 884
Tsp45I GTSAC 2 cut(s) 118, 1138
TspDTI ATGAA 4 cut(s) 528, 795, 1130, 1134
TspRI CASTG 1 cut(s) 401
Tth111I GACNNNGTC 1 cut(s) 1144
Van91I CCANNNNNTGG 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 472
VpaK11BI GGWCC 2 cut(s) 403, 588
XagI CCTNNNNNAGG 1 cut(s) 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1139
XhoI CTCGAG 1 cut(s) 981
XmnI GAANNNNTTC 3 cut(s) 933, 975, 1017
XspI CTAG 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.