RLG00000021103

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006274
Physical Location & Seq
Forward (+)
71974556 .. 71976978
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021103

Sequence Viewer

Length: 1953 bp
ATGGCGGTCGAAATCCCGACGGATGCGATAAAGCAGCTGCAGATCTTGCTTCGAAAACAAGCCAAGATGTCGTCGTACGACCCAGACGACACGCCGTTGGCGAATCTCCCTTCTCCGGAAGAAACCATATCGGAGCTGGATCCGTCGCCGCCGTACCTCCGGTGCAAGCATTGCAAAGGCCGGCTTCTGAGAGGCGTAGCGTCGCTGAGTTGCGTCTTCTGTGGCGCAGAAACCTGCAAGGACCTGCCTCCTGATCCTGTCAATTTCCGCAACACTTTAGGGTTCCGGTGGTTGCTCAAGTCCTTGGCTCTAGATGGATCGGAGATAGTGGACCTACCAACTAAAGCGAATGAATCCAGTAGAGGCCAGAATGCAACGAAGGATGAGTTAACCGTGTCTGAACTTTTAAATGTAGAAATAAGATGGACTTCTAAGTCTGAGAAATCCGGGGCTGGGCTTTCGGATGAGAAGGCTCCCACTCGGCCTAAAAGTTTTTTGGATTTGGCTGGAGTGAATCTGGACAACTTCTTTTCTGAAGGAGAGAAAGATGCTGCTTTGAATGCATCTGAAGAGCCGTTTGAGCCGAGCTCTCAGAAGACTGCAACTACAGAGAGTAGTGCTTTCAAACGTAGTGAAAATCTTAGTTTGTTTGCGTCAGAAGAGCCGTTTGAGCCGAACAAACCAATTGCCACTACAGAGAGTAGTGCTTTCAAAGGTAGTGAAAATCTTAGTATATTTGCGTTTGAGCCAAGCAAACAGATTGCCACTACAGAGAGTGGTGCGTTCCAAGGTAGTGAAAATCTTAGTTTCTTTGCATCTGAGAAGCCGTTTGAGTCGGGCAAACAGATTACCACCACAGAGAGTACTGCTTTTCAAGGTCATGAAAATCTTAGCTTGTTTGAGAATGTGCAGCCTTCTGAGACAGCTGTGCAGTCTACTCAAGGCGAGAGCGGTGATTCCATTTCCAGTTGGCCGGCAAGTTTTCAGTCCGCTGCTTCTGAAAATGTTCCTCAAGAATCAAAATCACTTGACCCCTTTGTGGGTTCTACAGTTGATCTTTCTGCCCATATAGACACGGTGTTTGGATCTGTTGGGGACTCGACTAATGTAAAATCAAACCATAGTACATCCACGAGTAATGACTGGTTTTCAGATGATTTATTGAGTATTTCCAACTCTGGGATAGCTGGGCAGCCTCAACAGTTAGAATCACTTTCTACGGTGAAGGATGACAGAATAGCTGAAAATGCAAATAACTTATTATCTACTGGTGTTGATTGGGTTGAAGACACTCAATGGCAGACCACTAGCAAGGAGGCTCCTGATAATACGACTGCTGATGAGGATGATGATTCGTTTGGTGCTTGGAATGATTTTACTAGCTCGAGTAGTGCACAGAATCCATCTAGCTCTTCAAAACAAACTGTCAATCGGACAACAGCTGCTGACAAGAATTCAGTAACAAATCTGTTCAGCACAGCTAGCAATTCACAAGATGATGATTCGTTTGGTGCTTGGAATGATTTTACTAGCTTGAGTAGTGCACAGAATGGTTCTAGCTCTTCAAAACAAACTGTTGATCAGATGACACCTGCTGATGAGACTTCAGTAACAAATCTGTTCAGCACAGCTAGCAACTCACAGGACTTGGATTTTGGTAACTTTTCGCAGCCAGATCTCTCTGCTGGAGCAATTAGCAGTTCACATGGTTCTACAGTAGTGAATATATCCCAGCCAGAGGCTTCTGTTTTAGACAGGACGGCTGATGCGGGCCCAAAAGATGAAGATGCTGCGAAGGATGGAGGAAATATTTTTAGTGCACAAACAGGATCAAAGTCTGAAGATGTGGAGAAAATAATGTCACAGATGCATGATCTCTCTTTTATGCTTGAGAGCAATCTTTCAATACCTCCCAAGCGAGATGTACATTCACTTTCCCAAGATCACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

651

Amino Acids

69.69

Weight (kDa)

4.35

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7815 PF25122 51 - 77 2.9e-14 Domain of unknown function (DUF7815)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1600
AasI GACNNNNNNGTC 1 cut(s) 433
Acc36I ACCTGC 3 cut(s) 242, 252, 1600
AccBSI CCGCTC 1 cut(s) 951
AccI GTMKAC 1 cut(s) 935
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 6 cut(s) 5, 149, 268, 951, 990, 1769
AclWI GGATC 6 cut(s) 134, 147, 248, 325, 1093, 1837
AcoI YGGCCR 1 cut(s) 971
AcsI RAATTY 1 cut(s) 1453
AcuI CTGAAG 4 cut(s) 555, 588, 1590, 1860
AfaI GTAC 5 cut(s) 77, 155, 865, 1126, 1926
AfiI CCNNNNNNNGG 6 cut(s) 115, 453, 1039, 1040, 1179, 1738
AgsI TTSAA 8 cut(s) 559, 625, 712, 875, 1286, 1416, 1566, 1905
Alw21I GWGCWC 4 cut(s) 590, 1396, 1546, 1822
Alw26I GTCTC 2 cut(s) 914, 1595
Alw44I GTGCAC 3 cut(s) 1392, 1542, 1818
AlwI GGATC 6 cut(s) 134, 147, 248, 325, 1093, 1837
AlwNI CAGNNNCTG 1 cut(s) 1445
Ama87I CYCGRG 1 cut(s) 1384
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 5 cut(s) 178, 364, 482, 971, 1771
ApaI GGGCCC 1 cut(s) 1775
ApaLI GTGCAC 3 cut(s) 1392, 1542, 1818
ApeKI GCWGC 9 cut(s) 34, 37, 551, 910, 992, 1192, 1442, 1669, 1790
ApoI RAATTY 1 cut(s) 1453
ArsI GACNNNNNNTTYG 4 cut(s) 1064, 1096, 1637, 1669
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 4 cut(s) 241, 331, 1771, 1772
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 2 cut(s) 965, 1234
AsuII TTCGAA 1 cut(s) 52
AsuNHI GCTAGC 2 cut(s) 1481, 1631
AvaI CYCGRG 1 cut(s) 1384
AvaII GGWCC 2 cut(s) 241, 331
BaeGI GKGCMC 4 cut(s) 1396, 1546, 1775, 1822
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 2 cut(s) 590, 1775
BauI CACGAG 1 cut(s) 1132
BbsI GAAGAC 3 cut(s) 208, 602, 1293
Bbv12I GWGCWC 4 cut(s) 590, 1396, 1546, 1822
BbvI GCAGC 9 cut(s) 24, 46, 538, 922, 979, 1204, 1429, 1681, 1777
BccI CCATC 4 cut(s) 308, 417, 1411, 1793
BceAI ACGGC 6 cut(s) 79, 136, 559, 649, 811, 1776
BclI TGATCA 1 cut(s) 1579
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 914, 1595
BfaI CTAG 8 cut(s) 311, 1308, 1380, 1407, 1482, 1530, 1557, 1632
BfmI CTRYAG 6 cut(s) 38, 606, 693, 768, 1047, 1713
BfuAI ACCTGC 3 cut(s) 242, 252, 1600
BglII AGATCT 2 cut(s) 42, 1675
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 2 cut(s) 241, 331
BmeT110I CYCGRG 1 cut(s) 1384
BmgT120I GGNCC 4 cut(s) 241, 331, 1771, 1772
BmiI GGNNCC 5 cut(s) 141, 284, 474, 1320, 1773
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 7 cut(s) 13, 538, 572, 824, 1756, 1777, 1857
BmtI GCTAGC 2 cut(s) 1485, 1635
BpiI GAAGAC 3 cut(s) 208, 602, 1293
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 2 cut(s) 528, 1707
Bpu14I TTCGAA 1 cut(s) 52
BpuEI CTTGAG 5 cut(s) 281, 924, 996, 1555, 1910
BpuMI CCSGG 1 cut(s) 448
BsaJI CCNNGG 3 cut(s) 303, 447, 787
BsaWI WCCGGW 3 cut(s) 115, 159, 285
Bsc4I CCNNNNNNNGG 6 cut(s) 115, 453, 1039, 1040, 1179, 1738
Bse118I RCCGGY 2 cut(s) 180, 973
Bse1I ACTGG 4 cut(s) 357, 966, 1148, 1273
Bse3DI GCAATG 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 115
BseDI CCNNGG 3 cut(s) 303, 447, 787
BseGI GGATG 7 cut(s) 28, 388, 469, 1127, 1234, 1351, 1804
BseLI CCNNNNNNNGG 6 cut(s) 115, 453, 1039, 1040, 1179, 1738
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 6 cut(s) 179, 197, 429, 605, 810, 909
BseNI ACTGG 4 cut(s) 357, 966, 1148, 1273
BseSI GKGCMC 4 cut(s) 1396, 1546, 1775, 1822
BseXI GCAGC 9 cut(s) 24, 46, 538, 922, 979, 1204, 1429, 1681, 1777
BseYI CCCAGC 3 cut(s) 452, 1187, 1731
BsgI GTGCAG 2 cut(s) 929, 950
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 5 cut(s) 180, 366, 484, 973, 1773
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 4 cut(s) 590, 1396, 1546, 1822
BsiHKCI CYCGRG 1 cut(s) 1384
BsiSI CCGG 6 cut(s) 116, 160, 181, 286, 447, 974
BsiWI CGTACG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 1109
BslI CCNNNNNNNGG 6 cut(s) 115, 453, 1039, 1040, 1179, 1738
BsmAI GTCTC 2 cut(s) 914, 1595
BsmFI GGGAC 1 cut(s) 1109
BsmI GAATGC 2 cut(s) 376, 565
BsnI GGCC 5 cut(s) 180, 366, 484, 973, 1773
BsoBI CYCGRG 1 cut(s) 1384
Bsp119I TTCGAA 1 cut(s) 52
Bsp120I GGGCCC 1 cut(s) 1771
Bsp1286I GDGCHC 5 cut(s) 590, 1396, 1546, 1775, 1822
Bsp13I TCCGGA 1 cut(s) 115
Bsp1407I TGTACA 1 cut(s) 1924
BspACI CCGC 6 cut(s) 5, 149, 268, 951, 990, 1769
BspANI GGCC 5 cut(s) 180, 366, 484, 973, 1773
BspCNI CTCAG 6 cut(s) 180, 198, 430, 604, 811, 910
BspEI TCCGGA 1 cut(s) 115
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 5 cut(s) 141, 284, 474, 1320, 1773
BspMAI CTGCAG 1 cut(s) 42
BspMI ACCTGC 3 cut(s) 242, 252, 1600
BspOI GCTAGC 2 cut(s) 1485, 1635
BspPI GGATC 6 cut(s) 134, 147, 248, 325, 1093, 1837
BspQI GCTCTTC 4 cut(s) 564, 654, 1417, 1567
BspT104I TTCGAA 1 cut(s) 52
BsrBI CCGCTC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 169
BsrFI RCCGGY 2 cut(s) 180, 973
BsrGI TGTACA 1 cut(s) 1924
BsrI ACTGG 4 cut(s) 357, 966, 1148, 1273
BssAI RCCGGY 2 cut(s) 180, 973
BssECI CCNNGG 3 cut(s) 303, 447, 787
BssSI CACGAG 1 cut(s) 1132
BssT1I CCWWGG 2 cut(s) 303, 787
Bst2BI CACGAG 1 cut(s) 1132
Bst4CI ACNGT 8 cut(s) 394, 1051, 1078, 1203, 1222, 1426, 1576, 1717
Bst6I CTCTTC 4 cut(s) 564, 654, 1417, 1567
BstAPI GCANNNNNTGC 2 cut(s) 46, 171
BstAUI TGTACA 1 cut(s) 1924
BstBI TTCGAA 1 cut(s) 52
BstC8I GCNNGC 6 cut(s) 167, 182, 975, 1483, 1633, 1771
BstF5I GGATG 7 cut(s) 28, 388, 469, 1127, 1234, 1351, 1804
BstHHI GCGC 1 cut(s) 227
BstMAI GTCTC 2 cut(s) 914, 1595
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 8 cut(s) 46, 171, 560, 580, 670, 1247, 1482, 1632
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 6 cut(s) 38, 606, 693, 768, 1047, 1713
BstSLI GKGCMC 4 cut(s) 1396, 1546, 1775, 1822
BstV1I GCAGC 9 cut(s) 24, 46, 538, 922, 979, 1204, 1429, 1681, 1777
BstV2I GAAGAC 3 cut(s) 208, 602, 1293
BstX2I RGATCY 4 cut(s) 42, 139, 1085, 1675
BstYI RGATCY 4 cut(s) 42, 139, 1085, 1675
BsuRI GGCC 5 cut(s) 180, 366, 484, 973, 1773
BtsCI GGATG 7 cut(s) 28, 388, 469, 1127, 1234, 1351, 1804
BveI ACCTGC 3 cut(s) 242, 252, 1600
Cac8I GCNNGC 6 cut(s) 167, 182, 975, 1483, 1633, 1771
CaiI CAGNNNCTG 1 cut(s) 1445
CciI TCATGA 1 cut(s) 880
CfoI GCGC 1 cut(s) 227
Cfr10I RCCGGY 2 cut(s) 180, 973
Cfr13I GGNCC 4 cut(s) 241, 331, 1771, 1772
CseI GACGC 3 cut(s) 189, 202, 642
Csp6I GTAC 5 cut(s) 76, 154, 864, 1125, 1925
CviAII CATG 3 cut(s) 881, 1706, 1871
CviQI GTAC 5 cut(s) 76, 154, 864, 1125, 1925
DraI TTTAAA 1 cut(s) 408
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 971
Eam1104I CTCTTC 4 cut(s) 564, 654, 1417, 1567
EarI CTCTTC 4 cut(s) 564, 654, 1417, 1567
Ecl136II GAGCTC 1 cut(s) 588
Eco130I CCWWGG 2 cut(s) 303, 787
Eco24I GRGCYC 2 cut(s) 590, 1775
Eco47I GGWCC 2 cut(s) 241, 331
Eco53kI GAGCTC 1 cut(s) 588
Eco57I CTGAAG 4 cut(s) 555, 588, 1590, 1860
Eco88I CYCGRG 1 cut(s) 1384
EcoICRI GAGCTC 1 cut(s) 588
EcoO109I RGGNCCY 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 1453
EcoT14I CCWWGG 2 cut(s) 303, 787
EcoT22I ATGCAT 2 cut(s) 565, 1872
EcoT38I GRGCYC 2 cut(s) 590, 1775
ErhI CCWWGG 2 cut(s) 303, 787
FaeI CATG 3 cut(s) 884, 1709, 1874
FalI AAGNNNNNCTT 4 cut(s) 168, 200, 412, 444
FaqI GGGAC 1 cut(s) 1109
FatI CATG 3 cut(s) 880, 1705, 1870
FauI CCCGC 1 cut(s) 1762
FbaI TGATCA 1 cut(s) 1579
FblI GTMKAC 1 cut(s) 935
FokI GGATG 7 cut(s) 35, 395, 476, 1114, 1241, 1358, 1811
FriOI GRGCYC 2 cut(s) 590, 1775
FspBI CTAG 8 cut(s) 311, 1308, 1380, 1407, 1482, 1530, 1557, 1632
GlaI GCGC 1 cut(s) 226
GsaI CCCAGC 3 cut(s) 456, 1191, 1735
GsuI CTGGAG 2 cut(s) 528, 1707
HaeIII GGCC 5 cut(s) 180, 366, 484, 973, 1773
HapII CCGG 6 cut(s) 116, 160, 181, 286, 447, 974
HgaI GACGC 3 cut(s) 189, 202, 642
HhaI GCGC 1 cut(s) 227
Hin1II CATG 3 cut(s) 884, 1709, 1874
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HpaI GTTAAC 1 cut(s) 390
HpaII CCGG 6 cut(s) 116, 160, 181, 286, 447, 974
HphI GGTGA 2 cut(s) 965, 1234
Hpy166II GTNNAC 7 cut(s) 331, 390, 936, 1394, 1544, 1703, 1820
Hpy188III TCNNGA 8 cut(s) 16, 116, 251, 311, 518, 881, 1013, 1322
Hpy8I GTNNAC 7 cut(s) 331, 390, 936, 1394, 1544, 1703, 1820
Hpy99I CGWCG 4 cut(s) 22, 76, 148, 205
HpyAV CCTTC 7 cut(s) 120, 373, 463, 530, 924, 1219, 1789
HpyCH4III ACNGT 8 cut(s) 394, 1051, 1078, 1203, 1222, 1426, 1576, 1717
HpyCH4IV ACGT 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 171, 560, 580, 670, 1247, 1482, 1632
HpySE526I ACGT 1 cut(s) 628
Hsp92II CATG 3 cut(s) 884, 1709, 1874
HspAI GCGC 1 cut(s) 225
Kpn2I TCCGGA 1 cut(s) 115
KroI GCCGGC 2 cut(s) 180, 973
KroNI GCCGGC 2 cut(s) 182, 975
Ksp22I TGATCA 1 cut(s) 1579
KspAI GTTAAC 1 cut(s) 390
LguI GCTCTTC 4 cut(s) 564, 654, 1417, 1567
LmnI GCTCC 4 cut(s) 133, 478, 1324, 1688
Lsp1109I GCAGC 9 cut(s) 24, 46, 538, 922, 979, 1204, 1429, 1681, 1777
LweI GCATC 7 cut(s) 13, 538, 572, 824, 1756, 1777, 1857
MaeI CTAG 8 cut(s) 311, 1308, 1380, 1407, 1482, 1530, 1557, 1632
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 4 cut(s) 1459, 1609, 1658, 1860
MbiI CCGCTC 1 cut(s) 951
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 4 cut(s) 42, 139, 1085, 1675
MhlI GDGCHC 5 cut(s) 590, 1396, 1546, 1775, 1822
MluCI AATT 5 cut(s) 262, 684, 1453, 1486, 1692
MlyI GAGTC 2 cut(s) 842, 1091
MmeI TCCRAC 1 cut(s) 1197
Mph1103I ATGCAT 2 cut(s) 565, 1872
MroI TCCGGA 1 cut(s) 115
MroNI GCCGGC 2 cut(s) 180, 973
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 2 cut(s) 389, 407
MspA1I CMGCKG 4 cut(s) 37, 926, 992, 1442
MspI CCGG 6 cut(s) 116, 160, 181, 286, 447, 974
MspR9I CCNGG 1 cut(s) 448
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 2 cut(s) 376, 565
MwoI GCNNNNNNNGC 8 cut(s) 46, 171, 560, 580, 670, 1247, 1482, 1632
NaeI GCCGGC 2 cut(s) 182, 975
NciI CCSGG 1 cut(s) 448
NgoMIV GCCGGC 2 cut(s) 180, 973
NheI GCTAGC 2 cut(s) 1481, 1631
NlaIII CATG 3 cut(s) 884, 1709, 1874
NlaIV GGNNCC 5 cut(s) 141, 284, 474, 1320, 1773
NmeAIII GCCGAG 2 cut(s) 460, 609
NmuCI GTSAC 1 cut(s) 1860
NsiI ATGCAT 2 cut(s) 565, 1872
NspV TTCGAA 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 1384
PagI TCATGA 1 cut(s) 880
PaqCI CACCTGC 1 cut(s) 1600
PciSI GCTCTTC 4 cut(s) 564, 654, 1417, 1567
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 98
PctI GAATGC 2 cut(s) 376, 565
PdiI GCCGGC 2 cut(s) 182, 975
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 9 cut(s) 103, 353, 514, 956, 1016, 1208, 1352, 1399, 1502
Pfl23II CGTACG 1 cut(s) 75
PleI GAGTC 2 cut(s) 841, 1091
PpsI GAGTC 2 cut(s) 841, 1091
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 590
Psp5II RGGWCCY 1 cut(s) 241
PspFI CCCAGC 3 cut(s) 452, 1187, 1731
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 5 cut(s) 141, 284, 474, 1320, 1773
PspOMI GGGCCC 1 cut(s) 1771
PspPI GGNCC 4 cut(s) 241, 331, 1771, 1772
PspPPI RGGWCCY 1 cut(s) 241
PspXI VCTCGAGB 1 cut(s) 1384
PstI CTGCAG 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 1445
PsuI RGATCY 4 cut(s) 42, 139, 1085, 1675
PvuII CAGCTG 3 cut(s) 37, 926, 1442
RsaI GTAC 5 cut(s) 77, 155, 865, 1126, 1926
RsaNI GTAC 5 cut(s) 76, 154, 864, 1125, 1925
SacI GAGCTC 1 cut(s) 590
SapI GCTCTTC 4 cut(s) 564, 654, 1417, 1567
SaqAI TTAA 2 cut(s) 389, 407
Sau96I GGNCC 4 cut(s) 241, 331, 1771, 1772
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 2 cut(s) 842, 1091
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 5 cut(s) 590, 1396, 1546, 1775, 1822
SfaNI GCATC 7 cut(s) 13, 538, 572, 824, 1756, 1777, 1857
SfcI CTRYAG 6 cut(s) 38, 606, 693, 768, 1047, 1713
Sfr274I CTCGAG 1 cut(s) 1384
SfuI TTCGAA 1 cut(s) 52
SinI GGWCC 2 cut(s) 241, 331
SlaI CTCGAG 1 cut(s) 1384
SmlI CTYRAG 6 cut(s) 296, 939, 1011, 1384, 1534, 1889
SmoI CTYRAG 6 cut(s) 296, 939, 1011, 1384, 1534, 1889
Sse9I AATT 5 cut(s) 262, 684, 1453, 1486, 1692
SsiI CCGC 6 cut(s) 5, 149, 268, 951, 990, 1769
SspI AATATT 1 cut(s) 1810
SspMI CTAG 8 cut(s) 311, 1308, 1380, 1407, 1482, 1530, 1557, 1632
SstI GAGCTC 1 cut(s) 590
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 2 cut(s) 303, 787
TaaI ACNGT 8 cut(s) 394, 1051, 1078, 1203, 1222, 1426, 1576, 1717
TaiI ACGT 1 cut(s) 631
TaqI TCGA 4 cut(s) 9, 52, 1100, 1385
TasI AATT 5 cut(s) 262, 684, 1453, 1486, 1692
TatI WGTACW 3 cut(s) 863, 1124, 1924
TauI GCSGC 1 cut(s) 151
TfiI GAWTC 9 cut(s) 103, 353, 514, 956, 1016, 1208, 1352, 1399, 1502
Tru1I TTAA 2 cut(s) 389, 407
Tru9I TTAA 2 cut(s) 389, 407
TseFI GTSAC 1 cut(s) 1860
TseI GCWGC 9 cut(s) 34, 37, 551, 910, 992, 1192, 1442, 1669, 1790
Tsp45I GTSAC 1 cut(s) 1860
TspDTI ATGAA 3 cut(s) 366, 897, 1797
TspGWI ACGGA 2 cut(s) 35, 132
VneI GTGCAC 3 cut(s) 1392, 1542, 1818
VpaK11BI GGWCC 2 cut(s) 241, 331
XapI RAATTY 1 cut(s) 1453
XbaI TCTAGA 1 cut(s) 310
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 1384
XmiI GTMKAC 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 8 cut(s) 311, 1308, 1380, 1407, 1482, 1530, 1557, 1632
ZrmI AGTACT 1 cut(s) 865
Zsp2I ATGCAT 2 cut(s) 565, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.