MD17G1100500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006274
Physical Location & Seq
Reverse (-)
8534435 .. 8536645
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1100500.v1.1.491

Sequence Viewer

Length: 1476 bp
ATGGCGATGGAAATCCCGATGGATGTGATAAAGCAGGTGCAGATCTCGCTTCGGAAAGAAGCGGAGCTGTCGTCGTACGACCCGGACGACACGCCGCTGCCGAATCTACCTTTTGTCGAAGAAACACTAGCGGAGTTGGATCCATCCCCGCCGTACCTGAGGTGCAGGCATTGCAAAGGGCGGCTGCTGAGGGGCGTTCAGACGCTGACCTGCGTCTTCTGCGGCAGAGAACACTGCAAGGACCTCCCTCCCGACCCCATCAATTTCCGGGATACTTTTGGGTACCGTTGGCTGCTCCAGTACCTGAGCTTAAGTGGATCGGAGGTAGTGGATTTACCTGTTGGAACAAATGAATTAAACAGAGCACAGACTGCGCGGAAGGATGAGTTAAGTCTGTCTGATCTCTTAAATCTTGAAATAAAATGGACCTCTAAGCCAGAGAAAACTGAGACTGATCTCCCAAATGAGATCCCCAATCTCCTCAAATTTCCAGATTTGGCTGGAGTCAATCCCGACAACTTTTTCTTTGAAGGAAAGAAAGATGGTGCTTCGAGTAATTCCGAAGAGTTGTTCGGGTCTAGCACACAGATTGTCGGTAAAGAGAGTTTCCAAGGTCATGAAACTCTTAGGTTGTTTGAGAATGTAAAGCCTTTTGAGACAGTTGTGCAGACTACTGAAGGAGAGAGTGGTGATTCTAGTTGGGCGGCAAGTTTTCAGTTTGCTGCTTCTGAATCACAAGCTTCTGGAAACCTTCCTCAAGCTTCTGAAACCCTTCCTCTAGCTTCTGAAAACCTTCCTCAAGCTTCTGAAATACTTCCTCGAGCTTCTGAAAACCTCCCTCAAGCTTCTGAAACCTTTCCCCAAGAATCAAAATCCCTTGACCCCTTTGTGGGTTCCACAGTGGATCTTTCTTCTGCCCATTCTGCCCACATAGGCACAGTATTTGGATCTGTAGTGGACTCAACAAATGAAAAATCAAACCATAGTGTGACTGGGTCTGCATCCATGTCCTCCGACTGGTTTCAAAATGATCTGTTGGGCGTTTCCAACTCTGGGTTGTCTGGAGGGCCTGAACGGTTGGAAATCTTTGCTGAGATGAAGGGTGGAATAGCTGAAAATGTGAATAACTCTTTTCCTGATGGTGTCGATTGGGTTCAAGACAACCAATTGCAGATCACTAGCAACAAGGATCTTGATAGTAATACTGTTGTGGAGAATCATGATTCACTTGATGCTTGGAATGATTTCGCAAGCTCGAGTAATGCACCAAATGTTGTGGATAGTTCTGTAAAACAAAGTGGTGTCGATTGGGTTCAGGACAACCAATTGCAGACCACTAGCATTAAGCCTCTTGATAATAAGACTACTGATGCGGATGATGATTCATTCGACGCTTGGAATGATTTTGCTAGCTCGGATAATGCATCAAATCTTGCAGATAATTCTGTAAAACAAAGTATTAATCAGATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

492

Amino Acids

53.64

Weight (kDa)

4.3

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7815 PF25122 51 - 77 5.2e-14 Domain of unknown function (DUF7815)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 25
Acc36I ACCTGC 2 cut(s) 25, 218
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 282
AccII CGCG 1 cut(s) 376
AciI CCGC 9 cut(s) 62, 95, 131, 149, 181, 222, 376, 704, 1373
AclWI GGATC 7 cut(s) 134, 147, 325, 463, 912, 955, 1197
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 4 cut(s) 77, 155, 284, 302
AfiI CCNNNNNNNGG 4 cut(s) 889, 890, 1017, 1053
AflII CTTAAG 1 cut(s) 310
AgsI TTSAA 4 cut(s) 416, 530, 1025, 1157
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 2 cut(s) 443, 650
AlwI GGATC 7 cut(s) 134, 147, 325, 463, 912, 955, 1197
AlwNI CAGNNNCTG 2 cut(s) 205, 304
Ama87I CYCGRG 2 cut(s) 819, 1255
AoxI GGCC 1 cut(s) 1067
ApeKI GCWGC 4 cut(s) 97, 184, 292, 722
ApoI RAATTY 1 cut(s) 485
AseI ATTAAT 1 cut(s) 1461
Asp700I GAANNNNTTC 5 cut(s) 771, 792, 813, 855, 1244
Asp718I GGTACC 1 cut(s) 282
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 241, 426, 1067
AsuC2I CCSGG 2 cut(s) 83, 269
AsuHPI GGTGA 1 cut(s) 701
AsuNHI GCTAGC 1 cut(s) 1409
AvaI CYCGRG 2 cut(s) 819, 1255
AvaII GGWCC 2 cut(s) 241, 426
AxyI CCTNAGG 1 cut(s) 158
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 367
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 84, 171, 279, 709
BccI CCATC 5 cut(s) 13, 151, 266, 536, 1133
BceAI ACGGC 1 cut(s) 136
BciVI GTATCC 1 cut(s) 265
BcnI CCSGG 2 cut(s) 83, 269
BcoDI GTCTC 2 cut(s) 443, 650
BfaI CTAG 7 cut(s) 128, 579, 696, 779, 1179, 1338, 1410
BfmI CTRYAG 1 cut(s) 951
BfrI CTTAAG 1 cut(s) 310
BfuAI ACCTGC 2 cut(s) 25, 218
BfuI GTATCC 1 cut(s) 265
BglII AGATCT 1 cut(s) 42
BisI GCNGC 8 cut(s) 95, 98, 182, 185, 223, 293, 705, 723
BlsI GCNGC 8 cut(s) 96, 99, 183, 186, 224, 294, 706, 724
Bme1390I CCNGG 2 cut(s) 83, 269
Bme18I GGWCC 2 cut(s) 241, 426
BmeT110I CYCGRG 2 cut(s) 819, 1255
BmgT120I GGNCC 3 cut(s) 241, 426, 1067
BmiI GGNNCC 3 cut(s) 141, 284, 895
BmrFI CCNGG 2 cut(s) 83, 269
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 4 cut(s) 1010, 1222, 1360, 1433
BmtI GCTAGC 1 cut(s) 1413
BmuI ACTGGG 1 cut(s) 1002
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 3 cut(s) 281, 522, 1083
Bpu10I CCTNAGC 2 cut(s) 188, 305
BpuEI CTTGAG 3 cut(s) 741, 783, 825
BpuMI CCSGG 2 cut(s) 83, 269
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 610
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 4 cut(s) 889, 890, 1017, 1053
Bse1I ACTGG 3 cut(s) 298, 997, 1022
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 169
Bse8I GATNNNNATC 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 610
BseGI GGATG 5 cut(s) 28, 143, 388, 1001, 1381
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 889, 890, 1017, 1053
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 5 cut(s) 149, 179, 296, 438, 1083
BseNI ACTGG 3 cut(s) 298, 997, 1022
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 4 cut(s) 84, 171, 279, 709
BsgI GTGCAG 3 cut(s) 59, 184, 686
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 1 cut(s) 1069
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 367
BsiHKCI CYCGRG 2 cut(s) 819, 1255
BsiSI CCGG 2 cut(s) 83, 268
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 889, 890, 1017, 1053
BsmAI GTCTC 2 cut(s) 443, 650
BsnI GGCC 1 cut(s) 1069
BsoBI CYCGRG 2 cut(s) 819, 1255
Bsp1286I GDGCHC 1 cut(s) 367
BspACI CCGC 9 cut(s) 62, 95, 131, 149, 181, 222, 376, 704, 1373
BspANI GGCC 1 cut(s) 1069
BspCNI CTCAG 5 cut(s) 150, 180, 297, 439, 1084
BspFNI CGCG 1 cut(s) 376
BspHI TCATGA 2 cut(s) 616, 1219
BspLI GGNNCC 3 cut(s) 141, 284, 895
BspMI ACCTGC 2 cut(s) 25, 218
BspOI GCTAGC 1 cut(s) 1413
BspPI GGATC 7 cut(s) 134, 147, 325, 463, 912, 955, 1197
BspT107I GGYRCC 1 cut(s) 282
BspTI CTTAAG 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 3 cut(s) 298, 997, 1022
BssECI CCNNGG 1 cut(s) 610
BssT1I CCWWGG 1 cut(s) 610
Bst4CI ACNGT 6 cut(s) 287, 661, 901, 940, 1077, 1207
Bst6I CTCTTC 1 cut(s) 558
BstAFI CTTAAG 1 cut(s) 310
BstAPI GCANNNNNTGC 2 cut(s) 171, 371
BstC8I GCNNGC 3 cut(s) 167, 1252, 1411
BstDEI CTNAG 7 cut(s) 158, 188, 305, 432, 447, 626, 1092
BstF5I GGATG 5 cut(s) 28, 143, 388, 1001, 1381
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 376
BstMAI GTCTC 2 cut(s) 443, 650
BstMWI GCNNNNNNNGC 5 cut(s) 46, 171, 219, 371, 923
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 2 cut(s) 81, 267
BstSFI CTRYAG 1 cut(s) 951
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 4 cut(s) 84, 171, 279, 709
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 6 cut(s) 42, 139, 468, 904, 947, 1189
BstYI RGATCY 6 cut(s) 42, 139, 468, 904, 947, 1189
Bsu36I CCTNAGG 1 cut(s) 158
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 1069
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 28, 143, 388, 1001, 1381
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 232, 906
BveI ACCTGC 2 cut(s) 25, 218
Cac8I GCNNGC 3 cut(s) 167, 1252, 1411
CaiI CAGNNNCTG 2 cut(s) 205, 304
CciI TCATGA 2 cut(s) 616, 1219
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 241, 426, 1067
CseI GACGC 3 cut(s) 202, 211, 1400
Csp6I GTAC 4 cut(s) 76, 154, 283, 301
CspCI CAANNNNNGTGG 2 cut(s) 1257, 1292
CviAII CATG 4 cut(s) 617, 1006, 1220, 1473
CviQI GTAC 4 cut(s) 76, 154, 283, 301
DdeI CTNAG 7 cut(s) 158, 188, 305, 432, 447, 626, 1092
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 610
Eco47I GGWCC 2 cut(s) 241, 426
Eco57I CTGAAG 1 cut(s) 696
Eco81I CCTNAGG 1 cut(s) 158
Eco88I CYCGRG 2 cut(s) 819, 1255
EcoO109I RGGNCCY 2 cut(s) 241, 1067
EcoT14I CCWWGG 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 1426
ErhI CCWWGG 1 cut(s) 610
FaeI CATG 4 cut(s) 620, 1009, 1223, 1476
FaiI YATR 6 cut(s) 618, 932, 984, 1007, 1221, 1474
FatI CATG 4 cut(s) 616, 1005, 1219, 1472
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 8 cut(s) 95, 98, 182, 185, 223, 293, 705, 723
FokI GGATG 5 cut(s) 35, 130, 395, 988, 1388
Fsp4HI GCNGC 8 cut(s) 95, 98, 182, 185, 223, 293, 705, 723
FspBI CTAG 7 cut(s) 128, 579, 696, 779, 1179, 1338, 1410
GlaI GCGC 1 cut(s) 375
GluI GCNGC 8 cut(s) 95, 98, 182, 185, 223, 293, 705, 723
GsuI CTGGAG 3 cut(s) 281, 522, 1083
HaeIII GGCC 1 cut(s) 1069
HapII CCGG 2 cut(s) 83, 268
HgaI GACGC 3 cut(s) 202, 211, 1400
HhaI GCGC 1 cut(s) 376
Hin1II CATG 4 cut(s) 620, 1009, 1223, 1476
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HindIII AAGCTT 4 cut(s) 738, 759, 801, 843
HinfI GANTC 9 cut(s) 103, 504, 692, 731, 866, 959, 1216, 1223, 1382
HpaII CCGG 2 cut(s) 83, 268
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 1 cut(s) 958
Hpy8I GTNNAC 1 cut(s) 958
Hpy99I CGWCG 2 cut(s) 76, 1394
HpyAV CCTTC 7 cut(s) 373, 524, 671, 761, 782, 803, 1093
HpyCH4III ACNGT 6 cut(s) 287, 661, 901, 940, 1077, 1207
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 171, 219, 371, 923
HpyF3I CTNAG 7 cut(s) 158, 188, 305, 432, 447, 626, 1092
Hsp92II CATG 4 cut(s) 620, 1009, 1223, 1476
HspAI GCGC 1 cut(s) 374
KpnI GGTACC 1 cut(s) 286
LmnI GCTCC 2 cut(s) 64, 300
Lsp1109I GCAGC 4 cut(s) 84, 171, 279, 709
LweI GCATC 4 cut(s) 1010, 1222, 1360, 1433
MaeI CTAG 7 cut(s) 128, 579, 696, 779, 1179, 1338, 1410
MaeIII GTNAC 1 cut(s) 988
MboII GAAGA 4 cut(s) 131, 208, 575, 903
MfeI CAATTG 2 cut(s) 1166, 1325
MflI RGATCY 6 cut(s) 42, 139, 468, 904, 947, 1189
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 7 cut(s) 262, 353, 485, 556, 1166, 1325, 1441
MlyI GAGTC 2 cut(s) 513, 953
MmeI TCCRAC 5 cut(s) 117, 322, 1038, 1059, 1071
Mph1103I ATGCAT 1 cut(s) 1426
MroXI GAANNNNTTC 5 cut(s) 771, 792, 813, 855, 1244
MseI TTAA 6 cut(s) 311, 356, 389, 407, 1344, 1461
MspA1I CMGCKG 1 cut(s) 97
MspCI CTTAAG 1 cut(s) 310
MspI CCGG 2 cut(s) 83, 268
MspR9I CCNGG 2 cut(s) 83, 269
MunI CAATTG 2 cut(s) 1166, 1325
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 5 cut(s) 46, 171, 219, 371, 923
NciI CCSGG 2 cut(s) 83, 269
NheI GCTAGC 1 cut(s) 1409
NlaIII CATG 4 cut(s) 620, 1009, 1223, 1476
NlaIV GGNNCC 3 cut(s) 141, 284, 895
NmuCI GTSAC 1 cut(s) 988
NsiI ATGCAT 1 cut(s) 1426
PaeR7I CTCGAG 2 cut(s) 819, 1255
PagI TCATGA 2 cut(s) 616, 1219
PaqCI CACCTGC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 98
PdmI GAANNNNTTC 5 cut(s) 771, 792, 813, 855, 1244
PfeI GAWTC 7 cut(s) 103, 692, 731, 866, 1216, 1223, 1382
Pfl23II CGTACG 1 cut(s) 75
PflFI GACNNNGTC 1 cut(s) 994
PfoI TCCNGGA 1 cut(s) 267
PkrI GCNGC 8 cut(s) 96, 99, 183, 186, 224, 294, 706, 724
PleI GAGTC 2 cut(s) 512, 953
PpsI GAGTC 2 cut(s) 512, 953
PpuMI RGGWCCY 1 cut(s) 241
PshAI GACNNNNGTC 1 cut(s) 212
PshBI ATTAAT 1 cut(s) 1461
Psp5II RGGWCCY 1 cut(s) 241
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 141, 284, 895
PspPI GGNCC 3 cut(s) 241, 426, 1067
PspPPI RGGWCCY 1 cut(s) 241
PspXI VCTCGAGB 2 cut(s) 819, 1255
PstNI CAGNNNCTG 2 cut(s) 205, 304
PsuI RGATCY 6 cut(s) 42, 139, 468, 904, 947, 1189
PsyI GACNNNGTC 1 cut(s) 994
RsaI GTAC 4 cut(s) 77, 155, 284, 302
RsaNI GTAC 4 cut(s) 76, 154, 283, 301
SaqAI TTAA 6 cut(s) 311, 356, 389, 407, 1344, 1461
SatI GCNGC 8 cut(s) 95, 98, 182, 185, 223, 293, 705, 723
Sau96I GGNCC 3 cut(s) 241, 426, 1067
SchI GAGTC 2 cut(s) 513, 953
ScrFI CCNGG 2 cut(s) 83, 269
SduI GDGCHC 1 cut(s) 367
SfaNI GCATC 4 cut(s) 1010, 1222, 1360, 1433
SfcI CTRYAG 1 cut(s) 951
Sfr274I CTCGAG 2 cut(s) 819, 1255
SinI GGWCC 2 cut(s) 241, 426
SlaI CTCGAG 2 cut(s) 819, 1255
SmlI CTYRAG 6 cut(s) 310, 756, 798, 819, 840, 1255
SmoI CTYRAG 6 cut(s) 310, 756, 798, 819, 840, 1255
Sse9I AATT 7 cut(s) 262, 353, 485, 556, 1166, 1325, 1441
SsiI CCGC 9 cut(s) 62, 95, 131, 149, 181, 222, 376, 704, 1373
SspMI CTAG 7 cut(s) 128, 579, 696, 779, 1179, 1338, 1410
StyD4I CCNGG 2 cut(s) 81, 267
StyI CCWWGG 1 cut(s) 610
TaaI ACNGT 6 cut(s) 287, 661, 901, 940, 1077, 1207
TaqI TCGA 7 cut(s) 117, 551, 820, 1146, 1256, 1305, 1389
TasI AATT 7 cut(s) 262, 353, 485, 556, 1166, 1325, 1441
TauI GCSGC 4 cut(s) 97, 184, 225, 707
TfiI GAWTC 7 cut(s) 103, 692, 731, 866, 1216, 1223, 1382
Tru1I TTAA 6 cut(s) 311, 356, 389, 407, 1344, 1461
Tru9I TTAA 6 cut(s) 311, 356, 389, 407, 1344, 1461
TscAI CASTG 2 cut(s) 239, 906
TseFI GTSAC 1 cut(s) 988
TseI GCWGC 4 cut(s) 97, 184, 292, 722
Tsp45I GTSAC 1 cut(s) 988
TspDTI ATGAA 5 cut(s) 366, 633, 984, 1112, 1374
TspRI CASTG 2 cut(s) 239, 906
Tth111I GACNNNGTC 1 cut(s) 994
Vha464I CTTAAG 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 241, 426
VspI ATTAAT 1 cut(s) 1461
XapI RAATTY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XhoI CTCGAG 2 cut(s) 819, 1255
XmnI GAANNNNTTC 5 cut(s) 771, 792, 813, 855, 1244
XspI CTAG 7 cut(s) 128, 579, 696, 779, 1179, 1338, 1410
Zsp2I ATGCAT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.