Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008223

Physical Location & Seq

Genomic Coordinates Forward (+)

12382603 .. 12386511

Gene Structure 3909 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1138000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGTCATCCGGGAGTTCAACTTCTTTGAATGTGGAATTATCAAAGAAGATAGGATTTTTGGGTTTGAAACTATGGGTTGTAATTGGTATATGTGTTGGTGCATTTATTGTTTTGATCCTATGTTTCTTATCTATATGGGTTTCATTTCGGAGGAAGTCTCGAAGATCAGTGGACAAGTTCCCAGTCACCCAAATACCGAATGTCTCTAAAGATATCAACATTGATAAGATGGCGGCTCAGGGTACCCATGATCACCCTGATAATTTATTTCATTCAGTACATGATAAAGCAAGTGATAAGAATTCAGAGAAGATGTTAGTTCAATTAGGCACGAGCAAATCGATTGATCCTAATAACATGAGTCAGTGCAGCTCAATTTATCATCATGAGAGGGCACTTAGTTCACAGTCGGGGGAAGAAGGAAGCTCTGGGACTGTGCGAAAGCAGTCGTCATTGTCTTGTGGAGGACTTGTTGCAGCCTCTCCTTTAGTTGGCTTGCCAGAAGTTTCTCATCTTGGGTGGGGTCACTGGTTCACTCTTAGGGATCTGCAATTTGCTACAAATCGTTTTTCTGCAGAAAATGTGCTTGGTGAGGGTGGATATGGAGTTGTTTACAAGGGCAGGCTGATCAATGGAACTGAGGTGGCAGTGAAAAAACTCTTAAATAACTTGGGGCAGGCAGAGAAAGAATTTAGGGTTGAAGTGGAGGCCATTGGTCATGTTCGGCATAAGAATCTTGTGCGGCTCTTGGGTTACTGCATAGAAGGTGTTCACAGGATGCTGGTGTATGAATATGTGAACAATGGAAATCTAGAGCAATGGCTACATGGGGCCATGTGCCAACACGGCACCCTTACTTGGGAAGCTCGCATGAAGGTGATTCTTGGTACTGCTAAGGCGCTTTCTTATCTACATGAAGCTATAGAACCAAAAGTTGTCCACAGAGATATAAAATCAAGCAACATTTTGATTGATAACGAGTTCAATGCCAAGGTTTCTGATTTTGGACTGGCCAAACTCTTGGGTTCAGGAGAAAGTCACATCACGACTAGAGTCATGGGGACTTTTGGTTATGTGGCACCAGAATATGCTAATACTGGCTTGTTAAATGAGAAGAGCGACATCTACAGCTTTGGTGTCCTCCTACTTGAAGCAGTTACGGGAAGGGATCCTGTGGACTATGGCCGACCTGCTAATGAGGTCAATCTGGTTGAGTGGCTAAAGTTGATGGTAGGTGCAAGAAGAGCCGAGGAAGTTGTGGATCCAAACCTTGAAGTTAAACCCAGTACACGTGCCTTAAAACGTGCCCTTTTGGTTGCACTTCGGTGTGTTGATCATGATTCAGAGAACAGGCCCAAAATGAGCCAGGTTGTGCGTATGCTCGAAGCTGATGAATACCCATTTCGTGAGGATCGAAGGAACAGAAAGAGTCGTACAGCCAGCATGGATATGGAATCTATGAAGGAAAGTTCTGCCTCTGTCGACATAGAAAAGAAGCCAGGGGATCTGGAGAGCCAAATATCCGAGACAAGCCATGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

514

Amino Acids

56.89

Weight (kDa)

7.69

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	192 - 459	2.3e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	192 - 461	7.2e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008223)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT3G59110
fragaria_vesca	FvH4_6g38380 FvH4_6g38380
malus_domestica	MD09G1150400.v1.1 MD17G1138000.v1.1
prunus_persica	Prupe.3G174500_v2.0.a1
pyrus_communis	pycom09g07030 pycom17g13120
rosa_chinensis	RchiOBHm_Chr2g0151691
rosa_laevigata	RLG00000020569
rosa_multiflora	Rmu_sc0005294.1_g000046
rosa_roxburghii	Rroxscaffold_2G00096250
rosa_rugosa	Rorug02G0428100
rosa_samantha	Rh2AG488900 Rh2BG501600 Rh2CG475500 Rh2DG512700
rosa_wichuraiana	Rw2G040090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1198
Acc65I	GGTACC	1 cut(s)	242
AccB1I	GGYRCC	3 cut(s)	242, 848, 1078
AccI	GTMKAC	1 cut(s)	1482
AciI	CCGC	2 cut(s)	233, 742
AclWI	GGATC	9 cut(s)	109, 341, 552, 1163, 1176, 1256, 1269, 1419, 1512
AcoI	YGGCCR	2 cut(s)	1011, 1183
AcsI	RAATTY	2 cut(s)	301, 689
AcvI	CACGTG	1 cut(s)	1292
AfaI	GTAC	5 cut(s)	244, 279, 889, 1288, 1435
AfiI	CCNNNNNNNGG	3 cut(s)	491, 858, 859
AflIII	ACRYGT	1 cut(s)	1289
AgsI	TTSAA	8 cut(s)	18, 28, 67, 323, 701, 985, 1151, 1274
AjnI	CCWGG	2 cut(s)	1365, 1498
AjuI	GAANNNNNNNTTGG	2 cut(s)	570, 602
AleI	CACNNNNGTG	1 cut(s)	1324
AluBI	AGCT	6 cut(s)	372, 426, 866, 920, 1131, 1388
AluI	AGCT	6 cut(s)	372, 426, 866, 920, 1131, 1388
Alw26I	GTCTC	3 cut(s)	162, 208, 1520
AlwI	GGATC	9 cut(s)	109, 341, 552, 1163, 1176, 1256, 1269, 1419, 1512
AoxI	GGCC	5 cut(s)	708, 831, 1011, 1183, 1352
ApeKI	GCWGC	2 cut(s)	369, 476
ApoI	RAATTY	2 cut(s)	301, 689
Asp700I	GAANNNNTTC	1 cut(s)	768
Asp718I	GGTACC	1 cut(s)	242
AspLEI	GCGC	1 cut(s)	901
AspS9I	GGNCC	2 cut(s)	831, 1353
AsuC2I	CCSGG	1 cut(s)	10
AsuHPI	GGTGA	4 cut(s)	178, 245, 602, 889
BaeGI	GKGCMC	2 cut(s)	397, 1309
BalI	TGGCCA	1 cut(s)	1013
BamHI	GGATCC	2 cut(s)	1168, 1261
BanI	GGYRCC	3 cut(s)	242, 848, 1078
BauI	CACGAG	1 cut(s)	331
BbrPI	CACGTG	1 cut(s)	1292
BbvI	GCAGC	2 cut(s)	381, 488
BccI	CCATC	2 cut(s)	223, 1222
BceAI	ACGGC	1 cut(s)	862
BcgI	CGANNNNNNTGC	2 cut(s)	968, 1002
BciT130I	CCWGG	2 cut(s)	1367, 1500
BclI	TGATCA	3 cut(s)	250, 627, 1333
BcnI	CCSGG	1 cut(s)	10
BcoDI	GTCTC	3 cut(s)	162, 208, 1520
BfaI	CTAG	2 cut(s)	812, 1050
BfmI	CTRYAG	3 cut(s)	573, 921, 1126
BfoI	RGCGCY	1 cut(s)	902
BfuAI	ACCTGC	1 cut(s)	1198
BglI	GCCNNNNNGGC	1 cut(s)	846
BisI	GCNGC	4 cut(s)	234, 370, 477, 743
BlsI	GCNGC	4 cut(s)	235, 371, 478, 744
Bme1390I	CCNGG	3 cut(s)	10, 1367, 1500
BmgT120I	GGNCC	2 cut(s)	831, 1353
BmiI	GGNNCC	6 cut(s)	244, 832, 850, 1080, 1170, 1263
BmrFI	CCNGG	3 cut(s)	10, 1367, 1500
BmrI	ACTGGG	2 cut(s)	176, 1278
BmsI	GCATC	1 cut(s)	768
BmuI	ACTGGG	2 cut(s)	176, 1278
BoxI	GACNNNNGTC	1 cut(s)	1052
BplI	GAGNNNNNCTC	2 cut(s)	142, 174
BpmI	CTGGAG	1 cut(s)	1529
Bpu10I	CCTNAGC	2 cut(s)	237, 894
BpuMI	CCSGG	1 cut(s)	10
Bsa29I	ATCGAT	1 cut(s)	341
BsaAI	YACGTR	1 cut(s)	1292
BsaJI	CCNNGG	4 cut(s)	990, 1248, 1499, 1534
BsaXI	ACNNNNNCTCC	2 cut(s)	456, 486
Bsc4I	CCNNNNNNNGG	3 cut(s)	491, 858, 859
Bse1I	ACTGG	5 cut(s)	182, 533, 1014, 1102, 1284
Bse3DI	GCAATG	1 cut(s)	824
BseBI	CCWGG	2 cut(s)	1367, 1500
BseCI	ATCGAT	1 cut(s)	341
BseDI	CCNNGG	4 cut(s)	990, 1248, 1499, 1534
BseGI	GGATG	2 cut(s)	5, 783
BseLI	CCNNNNNNNGG	3 cut(s)	491, 858, 859
BseMI	GCAATG	1 cut(s)	824
BseMII	CTCAG	2 cut(s)	251, 630
BseNI	ACTGG	5 cut(s)	182, 533, 1014, 1102, 1284
BseSI	GKGCMC	2 cut(s)	397, 1309
BseXI	GCAGC	2 cut(s)	381, 488
BsgI	GTGCAG	1 cut(s)	388
BshFI	GGCC	5 cut(s)	710, 833, 1013, 1185, 1354
BshNI	GGYRCC	3 cut(s)	242, 848, 1078
BshVI	ATCGAT	1 cut(s)	341
BsiSI	CCGG	1 cut(s)	9
BslFI	GGGAC	2 cut(s)	445, 1075
BslI	CCNNNNNNNGG	3 cut(s)	491, 858, 859
BsmAI	GTCTC	3 cut(s)	162, 208, 1520
BsmFI	GGGAC	2 cut(s)	445, 1075
BsnI	GGCC	5 cut(s)	710, 833, 1013, 1185, 1354
Bsp1286I	GDGCHC	2 cut(s)	397, 1309
Bsp19I	CCATGG	1 cut(s)	1534
BspACI	CCGC	2 cut(s)	233, 742
BspANI	GGCC	5 cut(s)	710, 833, 1013, 1185, 1354
BspCNI	CTCAG	2 cut(s)	250, 631
BspDI	ATCGAT	1 cut(s)	341
BspHI	TCATGA	2 cut(s)	385, 1336
BspLI	GGNNCC	6 cut(s)	244, 832, 850, 1080, 1170, 1263
BspMAI	CTGCAG	1 cut(s)	577
BspMI	ACCTGC	1 cut(s)	1198
BspPI	GGATC	9 cut(s)	109, 341, 552, 1163, 1176, 1256, 1269, 1419, 1512
BspQI	GCTCTTC	2 cut(s)	1109, 1237
BspT107I	GGYRCC	3 cut(s)	242, 848, 1078
BsrDI	GCAATG	1 cut(s)	824
BsrI	ACTGG	5 cut(s)	182, 533, 1014, 1102, 1284
BssECI	CCNNGG	4 cut(s)	990, 1248, 1499, 1534
BssSI	CACGAG	1 cut(s)	331
BssT1I	CCWWGG	2 cut(s)	990, 1534
Bst2BI	CACGAG	1 cut(s)	331
Bst2UI	CCWGG	2 cut(s)	1367, 1500
Bst4CI	ACNGT	2 cut(s)	408, 436
Bst6I	CTCTTC	2 cut(s)	1109, 1237
BstBAI	YACGTR	1 cut(s)	1292
BstC8I	GCNNGC	5 cut(s)	497, 623, 678, 868, 1441
BstDEI	CTNAG	5 cut(s)	237, 398, 539, 639, 894
BstDSI	CCRYGG	1 cut(s)	1534
BstF5I	GGATG	2 cut(s)	5, 783
BstH2I	RGCGCY	1 cut(s)	902
BstHHI	GCGC	1 cut(s)	901
BstMAI	GTCTC	3 cut(s)	162, 208, 1520
BstMWI	GCNNNNNNNGC	2 cut(s)	846, 1244
BstNI	CCWGG	2 cut(s)	1367, 1500
BstPAI	GACNNNNGTC	1 cut(s)	1052
BstSCI	CCNGG	3 cut(s)	8, 1365, 1498
BstSFI	CTRYAG	3 cut(s)	573, 921, 1126
BstSLI	GKGCMC	2 cut(s)	397, 1309
BstV1I	GCAGC	2 cut(s)	381, 488
BstX2I	RGATCY	4 cut(s)	544, 1168, 1261, 1504
BstXI	CCANNNNNNTGG	1 cut(s)	1021
BstYI	RGATCY	4 cut(s)	544, 1168, 1261, 1504
Bsu15I	ATCGAT	1 cut(s)	341
BsuRI	GGCC	5 cut(s)	710, 833, 1013, 1185, 1354
BsuTUI	ATCGAT	1 cut(s)	341
BtgI	CCRYGG	1 cut(s)	1534
BtsCI	GGATG	2 cut(s)	5, 783
BtsI	GCAGTG	1 cut(s)	654
BtsIMutI	CAGTG	4 cut(s)	174, 371, 526, 654
BveI	ACCTGC	1 cut(s)	1198
Cac8I	GCNNGC	5 cut(s)	497, 623, 678, 868, 1441
CciI	TCATGA	2 cut(s)	385, 1336
CfoI	GCGC	1 cut(s)	901
Cfr13I	GGNCC	2 cut(s)	831, 1353
ClaI	ATCGAT	1 cut(s)	341
Csp6I	GTAC	5 cut(s)	243, 278, 888, 1287, 1434
CviQI	GTAC	5 cut(s)	243, 278, 888, 1287, 1434
DdeI	CTNAG	5 cut(s)	237, 398, 539, 639, 894
EaeI	YGGCCR	2 cut(s)	1011, 1183
Eam1104I	CTCTTC	2 cut(s)	1109, 1237
EarI	CTCTTC	2 cut(s)	1109, 1237
Eco130I	CCWWGG	2 cut(s)	990, 1534
Eco32I	GATATC	1 cut(s)	214
Eco72I	CACGTG	1 cut(s)	1292
EcoRI	GAATTC	1 cut(s)	301
EcoRII	CCWGG	2 cut(s)	1365, 1498
EcoRV	GATATC	1 cut(s)	214
EcoT14I	CCWWGG	2 cut(s)	990, 1534
ErhI	CCWWGG	2 cut(s)	990, 1534
FaqI	GGGAC	2 cut(s)	445, 1075
FbaI	TGATCA	3 cut(s)	250, 627, 1333
FblI	GTMKAC	1 cut(s)	1482
Fnu4HI	GCNGC	4 cut(s)	234, 370, 477, 743
FokI	GGATG	1 cut(s)	790
Fsp4HI	GCNGC	4 cut(s)	234, 370, 477, 743
FspBI	CTAG	2 cut(s)	812, 1050
GlaI	GCGC	1 cut(s)	900
GluI	GCNGC	4 cut(s)	234, 370, 477, 743
GsuI	CTGGAG	1 cut(s)	1529
HaeII	RGCGCY	1 cut(s)	902
HaeIII	GGCC	5 cut(s)	710, 833, 1013, 1185, 1354
HapII	CCGG	1 cut(s)	9
HhaI	GCGC	1 cut(s)	901
Hin6I	GCGC	1 cut(s)	899
HinP1I	GCGC	1 cut(s)	899
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HinfI	GANTC	7 cut(s)	361, 733, 880, 1053, 1340, 1429, 1454
HpaII	CCGG	1 cut(s)	9
HphI	GGTGA	4 cut(s)	178, 245, 602, 889
Hpy188I	TCNGA	5 cut(s)	150, 307, 1000, 1345, 1525
Hpy188III	TCNNGA	8 cut(s)	159, 386, 812, 1029, 1045, 1337, 1406, 1508
HpyAV	CCTTC	6 cut(s)	413, 758, 868, 1158, 1410, 1456
HpyCH4III	ACNGT	2 cut(s)	408, 436
HpyCH4IV	ACGT	2 cut(s)	1291, 1303
HpyCH4V	TGCA	8 cut(s)	101, 369, 476, 550, 575, 759, 1238, 1319
HpyF10VI	GCNNNNNNNGC	2 cut(s)	846, 1244
HpyF3I	CTNAG	5 cut(s)	237, 398, 539, 639, 894
HpySE526I	ACGT	2 cut(s)	1291, 1303
HspAI	GCGC	1 cut(s)	899
KpnI	GGTACC	1 cut(s)	246
Ksp22I	TGATCA	3 cut(s)	250, 627, 1333
LguI	GCTCTTC	2 cut(s)	1109, 1237
Lsp1109I	GCAGC	2 cut(s)	381, 488
LweI	GCATC	1 cut(s)	768
MaeI	CTAG	2 cut(s)	812, 1050
MaeII	ACGT	2 cut(s)	1291, 1303
MaeIII	GTNAC	5 cut(s)	184, 524, 752, 1037, 1156
MboII	GAAGA	6 cut(s)	58, 174, 322, 428, 1126, 1254
MflI	RGATCY	4 cut(s)	544, 1168, 1261, 1504
MhlI	GDGCHC	2 cut(s)	397, 1309
MlsI	TGGCCA	1 cut(s)	1013
MluCI	AATT	8 cut(s)	35, 81, 262, 301, 323, 375, 551, 689
MluNI	TGGCCA	1 cut(s)	1013
MlyI	GAGTC	3 cut(s)	370, 1062, 1438
Mox20I	TGGCCA	1 cut(s)	1013
MroXI	GAANNNNTTC	1 cut(s)	768
MscI	TGGCCA	1 cut(s)	1013
MseI	TTAA	4 cut(s)	662, 1106, 1278, 1298
MslI	CAYNNNNRTG	3 cut(s)	875, 1324, 1448
Msp20I	TGGCCA	1 cut(s)	1013
MspI	CCGG	1 cut(s)	9
MspR9I	CCNGG	3 cut(s)	10, 1367, 1500
MvaI	CCWGG	2 cut(s)	1367, 1500
MwoI	GCNNNNNNNGC	2 cut(s)	846, 1244
NciI	CCSGG	1 cut(s)	10
NcoI	CCATGG	1 cut(s)	1534
NlaIV	GGNNCC	6 cut(s)	244, 832, 850, 1080, 1170, 1263
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	3 cut(s)	184, 524, 1037
OliI	CACNNNNGTG	1 cut(s)	1324
PagI	TCATGA	2 cut(s)	385, 1336
PciSI	GCTCTTC	2 cut(s)	1109, 1237
PcsI	WCGNNNNNNNCGW	2 cut(s)	338, 1411
PdmI	GAANNNNTTC	1 cut(s)	768
PfeI	GAWTC	4 cut(s)	733, 880, 1340, 1454
PfoI	TCCNGGA	1 cut(s)	8
PkrI	GCNGC	4 cut(s)	235, 371, 478, 744
PleI	GAGTC	3 cut(s)	369, 1061, 1437
PmaCI	CACGTG	1 cut(s)	1292
PmlI	CACGTG	1 cut(s)	1292
PpsI	GAGTC	3 cut(s)	369, 1061, 1437
Ppu21I	YACGTR	1 cut(s)	1292
PshAI	GACNNNNGTC	1 cut(s)	1052
Psp6I	CCWGG	2 cut(s)	1365, 1498
PspCI	CACGTG	1 cut(s)	1292
PspGI	CCWGG	2 cut(s)	1365, 1498
PspN4I	GGNNCC	6 cut(s)	244, 832, 850, 1080, 1170, 1263
PspPI	GGNCC	2 cut(s)	831, 1353
PstI	CTGCAG	1 cut(s)	577
PsuI	RGATCY	4 cut(s)	544, 1168, 1261, 1504
RsaI	GTAC	5 cut(s)	244, 279, 889, 1288, 1435
RsaNI	GTAC	5 cut(s)	243, 278, 888, 1287, 1434
RseI	CAYNNNNRTG	3 cut(s)	875, 1324, 1448
SalI	GTCGAC	1 cut(s)	1481
SapI	GCTCTTC	2 cut(s)	1109, 1237
SaqAI	TTAA	4 cut(s)	662, 1106, 1278, 1298
SatI	GCNGC	4 cut(s)	234, 370, 477, 743
Sau96I	GGNCC	2 cut(s)	831, 1353
SchI	GAGTC	3 cut(s)	370, 1062, 1438
ScrFI	CCNGG	3 cut(s)	10, 1367, 1500
SduI	GDGCHC	2 cut(s)	397, 1309
SfaNI	GCATC	1 cut(s)	768
SfcI	CTRYAG	3 cut(s)	573, 921, 1126
SmiMI	CAYNNNNRTG	3 cut(s)	875, 1324, 1448
Sse9I	AATT	8 cut(s)	35, 81, 262, 301, 323, 375, 551, 689
SsiI	CCGC	2 cut(s)	233, 742
SspMI	CTAG	2 cut(s)	812, 1050
StyD4I	CCNGG	3 cut(s)	8, 1365, 1498
StyI	CCWWGG	2 cut(s)	990, 1534
TaaI	ACNGT	2 cut(s)	408, 436
TaiI	ACGT	2 cut(s)	1294, 1306
TaqI	TCGA	5 cut(s)	160, 341, 1383, 1414, 1482
TasI	AATT	8 cut(s)	35, 81, 262, 301, 323, 375, 551, 689
TatI	WGTACW	2 cut(s)	277, 1286
TauI	GCSGC	2 cut(s)	236, 745
TfiI	GAWTC	4 cut(s)	733, 880, 1340, 1454
Tru1I	TTAA	4 cut(s)	662, 1106, 1278, 1298
Tru9I	TTAA	4 cut(s)	662, 1106, 1278, 1298
TscAI	CASTG	4 cut(s)	174, 371, 533, 654
TseFI	GTSAC	3 cut(s)	184, 524, 1037
TseI	GCWGC	2 cut(s)	369, 476
Tsp45I	GTSAC	3 cut(s)	184, 524, 1037
TspDTI	ATGAA	7 cut(s)	132, 260, 804, 887, 930, 1407, 1475
TspRI	CASTG	4 cut(s)	174, 371, 533, 654
XapI	RAATTY	2 cut(s)	301, 689
XbaI	TCTAGA	1 cut(s)	811
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1447
XmiI	GTMKAC	1 cut(s)	1482
XmnI	GAANNNNTTC	1 cut(s)	768
XspI	CTAG	2 cut(s)	812, 1050

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.