Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0008223

Physical Location & Seq

Genomic Coordinates Reverse (-)

71091012 .. 71094311

Gene Structure 3300 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG488900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1362 bp

ATGTCGTCTGAGAGTTCTTTGAATGTGCAATTATCAAAGAAGATATTGGGTATAAAACTATGGGTTTTGATCTGTATAAGTGTTGGTGCATTTATTATTCTGATCCTATGTTTCTTATCTATCTGGGTGACATTTCGGAGGAAATCTAGAAAATCTGTAGACAAGTTTTCAGTCAACCAAATACCAAATGTCTCTAAAGATATCAGGATTGATAGGGTGGGGGCTCAGAGTACAAATGATCACCCTGATAATTTATTTCTTACAGTACATGATAAAGCAACTGATAAGAATTCAGAGAACATGTTAGCTCATTTGGGAATGAGCAAATCAAGTGACCCTGATAACATGAGTCAGTGCAGTTCAGTTTATCACCATGAGAGGGCATTTAGTTCACAGTCAGGGGACGAAGGAAGCTCCGGGACTGTGCGAAAGCAGTCGTCATTGTCACATGGAGGAGTTGTAACGGCCTCTCCTTTAATTGGTTTGCCAGAAGTTTCTCATCTTGGTTGGGGCCACTGGTTTACTCTTAGGGATCTGCAATTTGCTACGAATCGATTTTCTGCTGAAAATGTACTTGGTGAGGGTGGATATGGGGTTGTTTACAGGGGCAGACTGATAAATGGAACTGAGGTGGCAGTGAAAAAGCTTCTAAATAATATGGGGCAGGCGGAGAAAGAATTTAGGGTTGAAGTGGAGGCCATAGGCCATGTTCGGCATAAGAATCTTGTGCGGCTGCTTGGTTATTGCATTGAAGGGGTTCACAGGATGCTGGTGTATGAATATGTGAATAATGGAAACCTAGAACAATGGCTGCATGGGGCCATGCGCCAACAGCAGGGCACCCTTACTTGGGAGGCCCGCATGAAGGTGATTCTTGGTACTGCCAGAGCTTATGTGGCACCAGAATATGCTAATACTGGCTTGTTAAACGAGAAGAGCGACATCTACAGCTTTGGTGTCCTCCTGCTTGAAGCAGTTACTGGCAGGGACCCCGTGGACTATGGTCGGCCTGCTAATGAGGTGAATCTTGTTGAGTGGCTAAAAATGATGGTAGGTGCAAGAAGAGCAGAGGAGGTTGTGGATCCAAACCTTGAAGTAAAACCCACTACACGTTCTTTAAAACGTGCCCTTTTGGTTGCACTTAGGTGTGTTGATCATGATGCAGAGAACAGACCTAAAATGAGCCAGGTTGTACGTATGCTTGAAGCTGATGAATACCCATCTCGTGAGGATCGAAGGAACAGAAAGACTCGGACAACCAGCATGGAAATTGAATCTTTGAAGGAAAATTCTGTCTCAGGTGACACAGAAAACAAGCCAGAGGATCTGGAAAGCCAAGTAGCCGAGACAACTCATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

453

Amino Acids

50.75

Weight (kDa)

7.25

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	188 - 296	2.4e-17	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	188 - 281	2.3e-13	Protein kinase domain
Pkinase	PF00069	288 - 399	2e-06	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	297 - 401	1e-08	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008223)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT3G59110
fragaria_vesca	FvH4_6g38380 FvH4_6g38380
malus_domestica	MD09G1150400.v1.1 MD17G1138000.v1.1
prunus_persica	Prupe.3G174500_v2.0.a1
pyrus_communis	pycom09g07030 pycom17g13120
rosa_chinensis	RchiOBHm_Chr2g0151691
rosa_laevigata	RLG00000020569
rosa_multiflora	Rmu_sc0005294.1_g000046
rosa_roxburghii	Rroxscaffold_2G00096250
rosa_rugosa	Rorug02G0428100
rosa_samantha	Rh2AG488900 Rh2BG501600 Rh2CG475500 Rh2DG512700
rosa_wichuraiana	Rw2G040090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	839, 898
AccI	GTMKAC	1 cut(s)	159
AciI	CCGC	3 cut(s)	668, 730, 859
AclWI	GGATC	6 cut(s)	97, 540, 1076, 1089, 1239, 1332
AcsI	RAATTY	3 cut(s)	289, 677, 1288
AfaI	GTAC	5 cut(s)	232, 267, 573, 880, 1194
AfiI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
AflIII	ACRYGT	2 cut(s)	300, 1109
AgsI	TTSAA	8 cut(s)	22, 689, 752, 971, 1094, 1205, 1274, 1282
AjnI	CCWGG	1 cut(s)	1185
AjuI	GAANNNNNNNTTGG	2 cut(s)	558, 590
AleI	CACNNNNGTG	1 cut(s)	1144
AluBI	AGCT	6 cut(s)	308, 414, 646, 890, 951, 1208
AluI	AGCT	6 cut(s)	308, 414, 646, 890, 951, 1208
Alw26I	GTCTC	3 cut(s)	196, 1300, 1340
AlwI	GGATC	6 cut(s)	97, 540, 1076, 1089, 1239, 1332
AlwNI	CAGNNNCTG	1 cut(s)	980
AoxI	GGCC	7 cut(s)	465, 511, 696, 703, 819, 855, 1007
ApeKI	GCWGC	2 cut(s)	733, 811
ApoI	RAATTY	3 cut(s)	289, 677, 1288
Asp700I	GAANNNNTTC	1 cut(s)	756
AspLEI	GCGC	1 cut(s)	828
AspS9I	GGNCC	4 cut(s)	511, 819, 856, 988
AsuC2I	CCSGG	1 cut(s)	418
AsuHPI	GGTGA	7 cut(s)	139, 233, 362, 590, 880, 1033, 1313
AvaII	GGWCC	1 cut(s)	988
BaeGI	GKGCMC	2 cut(s)	842, 1129
BamHI	GGATCC	1 cut(s)	1081
BanI	GGYRCC	2 cut(s)	839, 898
BanII	GRGCYC	1 cut(s)	226
BauI	CACGAG	1 cut(s)	1224
BbvI	GCAGC	2 cut(s)	720, 798
BccI	CCATC	2 cut(s)	1042, 1228
BceAI	ACGGC	1 cut(s)	480
BciT130I	CCWGG	1 cut(s)	1187
BclI	TGATCA	2 cut(s)	238, 1153
BcnI	CCSGG	1 cut(s)	418
BcoDI	GTCTC	3 cut(s)	196, 1300, 1340
BfaI	CTAG	2 cut(s)	147, 800
BfmI	CTRYAG	2 cut(s)	156, 946
BisI	GCNGC	3 cut(s)	731, 734, 812
BlsI	GCNGC	3 cut(s)	732, 735, 813
Bme1390I	CCNGG	2 cut(s)	418, 1187
Bme18I	GGWCC	1 cut(s)	988
BmgT120I	GGNCC	4 cut(s)	511, 819, 856, 988
BmiI	GGNNCC	7 cut(s)	512, 820, 841, 900, 989, 990, 1083
BmrFI	CCNGG	2 cut(s)	418, 1187
BmsI	GCATC	2 cut(s)	756, 1150
BoxI	GACNNNNGTC	1 cut(s)	1002
BpuMI	CCSGG	1 cut(s)	418
Bsa29I	ATCGAT	1 cut(s)	553
BsaAI	YACGTR	1 cut(s)	1196
BsaJI	CCNNGG	1 cut(s)	993
BsaXI	ACNNNNNCTCC	4 cut(s)	444, 454, 474, 484
Bsc4I	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
Bse1I	ACTGG	3 cut(s)	521, 922, 985
BseBI	CCWGG	1 cut(s)	1187
BseCI	ATCGAT	1 cut(s)	553
BseDI	CCNNGG	1 cut(s)	993
BseGI	GGATG	1 cut(s)	771
BseLI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
BseMII	CTCAG	3 cut(s)	239, 618, 1311
BseNI	ACTGG	3 cut(s)	521, 922, 985
BseRI	GAGGAG	2 cut(s)	468, 1085
BseSI	GKGCMC	2 cut(s)	842, 1129
BseXI	GCAGC	2 cut(s)	720, 798
BsgI	GTGCAG	1 cut(s)	376
BshFI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1009
BshNI	GGYRCC	2 cut(s)	839, 898
BshVI	ATCGAT	1 cut(s)	553
BsiSI	CCGG	1 cut(s)	417
BslFI	GGGAC	3 cut(s)	416, 433, 1001
BslI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
BsmAI	GTCTC	3 cut(s)	196, 1300, 1340
BsmFI	GGGAC	3 cut(s)	416, 433, 1001
BsnI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1009
Bsp1286I	GDGCHC	3 cut(s)	226, 842, 1129
Bsp143I	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
BspACI	CCGC	3 cut(s)	668, 730, 859
BspANI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1009
BspCNI	CTCAG	3 cut(s)	238, 619, 1310
BspDI	ATCGAT	1 cut(s)	553
BspHI	TCATGA	1 cut(s)	1156
BspLI	GGNNCC	7 cut(s)	512, 820, 841, 900, 989, 990, 1083
BspPI	GGATC	6 cut(s)	97, 540, 1076, 1089, 1239, 1332
BspQI	GCTCTTC	2 cut(s)	929, 1057
BspT107I	GGYRCC	2 cut(s)	839, 898
BsrI	ACTGG	3 cut(s)	521, 922, 985
BssECI	CCNNGG	1 cut(s)	993
BssMI	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
BssSI	CACGAG	1 cut(s)	1224
Bst2BI	CACGAG	1 cut(s)	1224
Bst2UI	CCWGG	1 cut(s)	1187
Bst4CI	ACNGT	3 cut(s)	265, 396, 424
Bst6I	CTCTTC	2 cut(s)	929, 1057
BstBAI	YACGTR	1 cut(s)	1196
BstC8I	GCNNGC	3 cut(s)	666, 859, 1011
BstDEI	CTNAG	6 cut(s)	9, 225, 527, 627, 1142, 1297
BstDSI	CCRYGG	1 cut(s)	993
BstF5I	GGATG	1 cut(s)	771
BstHHI	GCGC	1 cut(s)	828
BstKTI	GATC	8 cut(s)	72, 105, 241, 535, 1084, 1156, 1234, 1327
BstMAI	GTCTC	3 cut(s)	196, 1300, 1340
BstMBI	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
BstMWI	GCNNNNNNNGC	3 cut(s)	832, 896, 1064
BstNI	CCWGG	1 cut(s)	1187
BstNSI	RCATGY	1 cut(s)	304
BstPAI	GACNNNNGTC	1 cut(s)	1002
BstSCI	CCNGG	2 cut(s)	416, 1185
BstSFI	CTRYAG	2 cut(s)	156, 946
BstSLI	GKGCMC	2 cut(s)	842, 1129
BstSNI	TACGTA	1 cut(s)	1196
BstV1I	GCAGC	2 cut(s)	720, 798
BstX2I	RGATCY	3 cut(s)	532, 1081, 1324
BstYI	RGATCY	3 cut(s)	532, 1081, 1324
Bsu15I	ATCGAT	1 cut(s)	553
BsuRI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1009
BsuTUI	ATCGAT	1 cut(s)	553
BtgI	CCRYGG	1 cut(s)	993
BtsCI	GGATG	1 cut(s)	771
BtsI	GCAGTG	1 cut(s)	642
BtsIMutI	CAGTG	3 cut(s)	359, 514, 642
Cac8I	GCNNGC	3 cut(s)	666, 859, 1011
CaiI	CAGNNNCTG	1 cut(s)	980
CciI	TCATGA	1 cut(s)	1156
CfoI	GCGC	1 cut(s)	828
Cfr13I	GGNCC	4 cut(s)	511, 819, 856, 988
ClaI	ATCGAT	1 cut(s)	553
Csp6I	GTAC	5 cut(s)	231, 266, 572, 879, 1193
CviQI	GTAC	5 cut(s)	231, 266, 572, 879, 1193
DdeI	CTNAG	6 cut(s)	9, 225, 527, 627, 1142, 1297
DpnI	GATC	8 cut(s)	71, 104, 240, 534, 1083, 1155, 1233, 1326
DpnII	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
DraI	TTTAAA	1 cut(s)	1119
Eam1104I	CTCTTC	2 cut(s)	929, 1057
EarI	CTCTTC	2 cut(s)	929, 1057
EciI	GGCGGA	1 cut(s)	683
Eco105I	TACGTA	1 cut(s)	1196
Eco24I	GRGCYC	1 cut(s)	226
Eco32I	GATATC	1 cut(s)	202
Eco47I	GGWCC	1 cut(s)	988
EcoO109I	RGGNCCY	1 cut(s)	988
EcoRI	GAATTC	1 cut(s)	289
EcoRII	CCWGG	1 cut(s)	1185
EcoRV	GATATC	1 cut(s)	202
EcoT22I	ATGCAT	1 cut(s)	1360
EcoT38I	GRGCYC	1 cut(s)	226
FaqI	GGGAC	3 cut(s)	416, 433, 1001
FauI	CCCGC	1 cut(s)	866
FbaI	TGATCA	2 cut(s)	238, 1153
FblI	GTMKAC	1 cut(s)	159
Fnu4HI	GCNGC	3 cut(s)	731, 734, 812
FokI	GGATG	1 cut(s)	778
FriOI	GRGCYC	1 cut(s)	226
Fsp4HI	GCNGC	3 cut(s)	731, 734, 812
FspBI	CTAG	2 cut(s)	147, 800
GlaI	GCGC	1 cut(s)	827
GluI	GCNGC	3 cut(s)	731, 734, 812
HaeIII	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1009
HapII	CCGG	1 cut(s)	417
HhaI	GCGC	1 cut(s)	828
Hin6I	GCGC	1 cut(s)	826
HinP1I	GCGC	1 cut(s)	826
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	7 cut(s)	349, 550, 721, 871, 1024, 1249, 1274
HpaII	CCGG	1 cut(s)	417
HphI	GGTGA	7 cut(s)	139, 233, 362, 590, 880, 1033, 1313
Hpy166II	GTNNAC	7 cut(s)	160, 175, 392, 522, 601, 760, 997
Hpy188I	TCNGA	6 cut(s)	10, 102, 138, 228, 295, 1254
Hpy188III	TCNNGA	5 cut(s)	147, 205, 1157, 1226, 1328
Hpy8I	GTNNAC	7 cut(s)	160, 175, 392, 522, 601, 760, 997
HpyAV	CCTTC	5 cut(s)	401, 746, 859, 1230, 1276
HpyCH4III	ACNGT	3 cut(s)	265, 396, 424
HpyCH4IV	ACGT	3 cut(s)	1111, 1123, 1195
HpyF10VI	GCNNNNNNNGC	3 cut(s)	832, 896, 1064
HpyF3I	CTNAG	6 cut(s)	9, 225, 527, 627, 1142, 1297
HpySE526I	ACGT	3 cut(s)	1111, 1123, 1195
HspAI	GCGC	1 cut(s)	826
KflI	GGGWCCC	1 cut(s)	988
Ksp22I	TGATCA	2 cut(s)	238, 1153
Kzo9I	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
LguI	GCTCTTC	2 cut(s)	929, 1057
LmnI	GCTCC	1 cut(s)	419
Lsp1109I	GCAGC	2 cut(s)	720, 798
LweI	GCATC	2 cut(s)	756, 1150
MaeI	CTAG	2 cut(s)	147, 800
MaeII	ACGT	3 cut(s)	1111, 1123, 1195
MaeIII	GTNAC	6 cut(s)	127, 332, 444, 460, 976, 1301
MalI	GATC	8 cut(s)	71, 104, 240, 534, 1083, 1155, 1233, 1326
MboI	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
MboII	GAAGA	3 cut(s)	52, 946, 1074
MflI	RGATCY	3 cut(s)	532, 1081, 1324
MhlI	GDGCHC	3 cut(s)	226, 842, 1129
MluCI	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1269, 1288
MlyI	GAGTC	2 cut(s)	358, 1243
Mph1103I	ATGCAT	1 cut(s)	1360
MroXI	GAANNNNTTC	1 cut(s)	756
MseI	TTAA	3 cut(s)	476, 926, 1118
MslI	CAYNNNNRTG	2 cut(s)	866, 1144
MspI	CCGG	1 cut(s)	417
MspR9I	CCNGG	2 cut(s)	418, 1187
MvaI	CCWGG	1 cut(s)	1187
MwoI	GCNNNNNNNGC	3 cut(s)	832, 896, 1064
NciI	CCSGG	1 cut(s)	418
NdeII	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
NlaIV	GGNNCC	7 cut(s)	512, 820, 841, 900, 989, 990, 1083
NmuCI	GTSAC	4 cut(s)	127, 332, 444, 1301
NsiI	ATGCAT	1 cut(s)	1360
NspI	RCATGY	1 cut(s)	304
OliI	CACNNNNGTG	1 cut(s)	1144
PagI	TCATGA	1 cut(s)	1156
PciI	ACATGT	1 cut(s)	300
PciSI	GCTCTTC	2 cut(s)	929, 1057
PcsI	WCGNNNNNNNCGW	2 cut(s)	936, 1231
PdmI	GAANNNNTTC	1 cut(s)	756
PfeI	GAWTC	5 cut(s)	550, 721, 871, 1024, 1274
PfoI	TCCNGGA	1 cut(s)	416
PkrI	GCNGC	3 cut(s)	732, 735, 813
PleI	GAGTC	2 cut(s)	357, 1243
PpsI	GAGTC	2 cut(s)	357, 1243
Ppu21I	YACGTR	1 cut(s)	1196
PpuMI	RGGWCCY	1 cut(s)	988
PscI	ACATGT	1 cut(s)	300
PshAI	GACNNNNGTC	1 cut(s)	1002
Psp5II	RGGWCCY	1 cut(s)	988
Psp6I	CCWGG	1 cut(s)	1185
PspGI	CCWGG	1 cut(s)	1185
PspN4I	GGNNCC	7 cut(s)	512, 820, 841, 900, 989, 990, 1083
PspPI	GGNCC	4 cut(s)	511, 819, 856, 988
PspPPI	RGGWCCY	1 cut(s)	988
PstNI	CAGNNNCTG	1 cut(s)	980
PsuI	RGATCY	3 cut(s)	532, 1081, 1324
RsaI	GTAC	5 cut(s)	232, 267, 573, 880, 1194
RsaNI	GTAC	5 cut(s)	231, 266, 572, 879, 1193
RseI	CAYNNNNRTG	2 cut(s)	866, 1144
SapI	GCTCTTC	2 cut(s)	929, 1057
SaqAI	TTAA	3 cut(s)	476, 926, 1118
SatI	GCNGC	3 cut(s)	731, 734, 812
Sau3AI	GATC	8 cut(s)	69, 102, 238, 532, 1081, 1153, 1231, 1324
Sau96I	GGNCC	4 cut(s)	511, 819, 856, 988
SchI	GAGTC	2 cut(s)	358, 1243
ScrFI	CCNGG	2 cut(s)	418, 1187
SduI	GDGCHC	3 cut(s)	226, 842, 1129
SfaNI	GCATC	2 cut(s)	756, 1150
SfcI	CTRYAG	2 cut(s)	156, 946
SinI	GGWCC	1 cut(s)	988
SmiMI	CAYNNNNRTG	2 cut(s)	866, 1144
SnaBI	TACGTA	1 cut(s)	1196
Sse9I	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1269, 1288
SsiI	CCGC	3 cut(s)	668, 730, 859
SspMI	CTAG	2 cut(s)	147, 800
StyD4I	CCNGG	2 cut(s)	416, 1185
TaaI	ACNGT	3 cut(s)	265, 396, 424
TaiI	ACGT	3 cut(s)	1114, 1126, 1198
TaqI	TCGA	2 cut(s)	553, 1234
TasI	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1269, 1288
TatI	WGTACW	3 cut(s)	230, 265, 571
TauI	GCSGC	1 cut(s)	733
TfiI	GAWTC	5 cut(s)	550, 721, 871, 1024, 1274
Tru1I	TTAA	3 cut(s)	476, 926, 1118
Tru9I	TTAA	3 cut(s)	476, 926, 1118
TscAI	CASTG	3 cut(s)	359, 521, 642
TseFI	GTSAC	4 cut(s)	127, 332, 444, 1301
TseI	GCWGC	2 cut(s)	733, 811
Tsp45I	GTSAC	4 cut(s)	127, 332, 444, 1301
TspDTI	ATGAA	3 cut(s)	792, 878, 1227
TspRI	CASTG	3 cut(s)	359, 521, 642
VpaK11BI	GGWCC	1 cut(s)	988
XapI	RAATTY	3 cut(s)	289, 677, 1288
XbaI	TCTAGA	1 cut(s)	146
XceI	RCATGY	1 cut(s)	304
XcmI	CCANNNNNNNNNTGG	1 cut(s)	892
XmiI	GTMKAC	1 cut(s)	159
XmnI	GAANNNNTTC	1 cut(s)	756
XspI	CTAG	2 cut(s)	147, 800
Zsp2I	ATGCAT	1 cut(s)	1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.