Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0008223

Physical Location & Seq

Genomic Coordinates Reverse (-)

66353589 .. 66356720

Gene Structure 3132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020569

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGTCGTCTGAGAGTTCTTTGAATGTGCAATTATCAAAGAAGATATTGGGTATAAAACTATGGGTTTTGATCTGTATAAGTGTTGGTGCATTTATTATTCTGATCCTATGTTTCTTATCTATCTGGGTGACATTTCGGAGGAAATCTAGAAAATCTATAGACAAGTTTTCAGTCAACCAAATACCAAATGTCTCTAAAGATATCAGGATTGATAGGGTGGGGGCTCAGAGTACACATGATCACCCTGATAATTTATTTCTTACAGTACATGATAAAGCAACTGATAAGAATTCAGAGAACATGTTAGCTCATTTGGGAATGAGCAAATCAAGTGACCCTGATAACATGAGTCAGTGCAGTTCAGTTTATCACCATGAGAGGGCATTTAGTTCACAGTCAGGGGACGAAGGAAGCTCCGGGACTGTGCGAAAGCAGTCATCATTGTCACATGGAGGAGTTGTAACGGCCTCTCCTTTAATTGGTTTGCCAGAAGTTTCTCATCTTGGTTGGGGCCACTGGTTTACTCTTAGGGATCTGCAATTTGCTACGAATCGATTTTCTGCTGAAAATGTACTTGGTGAGGGTGGATATGGGGTTGTTTACAGGGGCAGACTGATAAATGGAACTGAGGTGGCAGTGAAAAAGCTTCTAAATAATATGGGGCAGGCGGAGAAAGAATTTAGGGTTGAAGTGGAGGCCATAGGCCACGTTCGGCATAAGAATCTTGTGCGGCTGCTTGGTTATTGCATTGAAGGGGTTCACAGGATGCTGGTGTATGAATATGTGAATAATGGAAACCTAGAACAATGGCTGCATGGGGCCATGCGCCAACAGCAGGGCACCCTTACTTGGGAGGCCCGCATGAAGGTGATTCTTGGTACTGCCAGAGCGCTTTCTTATCTACACGAAGCTATCGAACCAAAAGTTATTCACAGAGATATAAAATCAAGCAACATTCTGATTGATGATGATTATGTGGCACCAGAATATGCTAATACTGGCTTGTTAAATGAGAAGAGCGACATCTACAGCTTTGGTGTCCTCCTGCTTGAAGCAGTTACTGGCAGGGACCCCGTGGACTATGGTCGGCCTGCTAATGAGGTGAATCTTGTTGAGTGGCTAAAAATGATGGTAGGTGCAAGAAGAGCAGAGGAGGTTGTGGATCCAAACCTTGAAGTAAAACCCAGTACACGTTCTTTAAAGCGTGCCCTTTTGGTTGCACTTAGGTGTGTTGATCATGATGCAGAGAACAGACCTAAAATGAGCCAGGTTGTACGTATGCTTGAAGCTGAGGAATACCCATCTCGTGAGGATCGAAGGAACAGAAAGACTCGGACAACCAGCATGGAAATTGAATCTTTGAAGGAAAATTCTGTCTCAGGTGACACAGAAAACAAGCCAGAGGATCTGGAAAGCCAAGTAGCCGAGACAACTCATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

481

Amino Acids

53.91

Weight (kDa)

6.64

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	188 - 324	2.4e-26	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	188 - 323	2e-25	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	325 - 428	2e-08	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008223)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT2G42960 AT3G59110
fragaria_vesca	FvH4_6g38380 FvH4_6g38380
malus_domestica	MD09G1150400.v1.1 MD17G1138000.v1.1
prunus_persica	Prupe.3G174500_v2.0.a1
pyrus_communis	pycom09g07030 pycom17g13120
rosa_chinensis	RchiOBHm_Chr2g0151691
rosa_laevigata	RLG00000020569
rosa_multiflora	Rmu_sc0005294.1_g000046
rosa_roxburghii	Rroxscaffold_2G00096250
rosa_rugosa	Rorug02G0428100
rosa_samantha	Rh2AG488900 Rh2BG501600 Rh2CG475500 Rh2DG512700
rosa_wichuraiana	Rw2G040090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	839, 979
AciI	CCGC	3 cut(s)	668, 730, 859
AclWI	GGATC	6 cut(s)	97, 540, 1157, 1170, 1320, 1413
AcsI	RAATTY	3 cut(s)	289, 677, 1369
AfaI	GTAC	6 cut(s)	232, 267, 573, 880, 1189, 1275
AfeI	AGCGCT	1 cut(s)	891
AfiI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
AflIII	ACRYGT	2 cut(s)	300, 1190
AgsI	TTSAA	8 cut(s)	22, 689, 752, 1052, 1175, 1286, 1355, 1363
AjnI	CCWGG	1 cut(s)	1266
AjuI	GAANNNNNNNTTGG	2 cut(s)	558, 590
AleI	CACNNNNGTG	1 cut(s)	1225
AluBI	AGCT	6 cut(s)	308, 414, 646, 911, 1032, 1289
AluI	AGCT	6 cut(s)	308, 414, 646, 911, 1032, 1289
Alw26I	GTCTC	3 cut(s)	196, 1381, 1421
AlwI	GGATC	6 cut(s)	97, 540, 1157, 1170, 1320, 1413
AlwNI	CAGNNNCTG	1 cut(s)	1061
Aor51HI	AGCGCT	1 cut(s)	891
AoxI	GGCC	7 cut(s)	465, 511, 696, 703, 819, 855, 1088
ApeKI	GCWGC	2 cut(s)	733, 811
ApoI	RAATTY	3 cut(s)	289, 677, 1369
Asp700I	GAANNNNTTC	1 cut(s)	756
AspLEI	GCGC	2 cut(s)	828, 892
AspS9I	GGNCC	4 cut(s)	511, 819, 856, 1069
AsuC2I	CCSGG	1 cut(s)	418
AsuHPI	GGTGA	7 cut(s)	139, 233, 362, 590, 880, 1114, 1394
AvaII	GGWCC	1 cut(s)	1069
BaeGI	GKGCMC	2 cut(s)	842, 1210
BamHI	GGATCC	1 cut(s)	1162
BanI	GGYRCC	2 cut(s)	839, 979
BanII	GRGCYC	1 cut(s)	226
BauI	CACGAG	1 cut(s)	1305
BbvCI	CCTCAGC	1 cut(s)	1290
BbvI	GCAGC	2 cut(s)	720, 798
BccI	CCATC	2 cut(s)	1123, 1309
BceAI	ACGGC	1 cut(s)	480
BciT130I	CCWGG	1 cut(s)	1268
BclI	TGATCA	2 cut(s)	238, 1234
BcnI	CCSGG	1 cut(s)	418
BcoDI	GTCTC	3 cut(s)	196, 1381, 1421
BfaI	CTAG	2 cut(s)	147, 800
BfmI	CTRYAG	2 cut(s)	156, 1027
BfoI	RGCGCY	1 cut(s)	893
BisI	GCNGC	3 cut(s)	731, 734, 812
BlsI	GCNGC	3 cut(s)	732, 735, 813
Bme1390I	CCNGG	2 cut(s)	418, 1268
Bme18I	GGWCC	1 cut(s)	1069
BmgT120I	GGNCC	4 cut(s)	511, 819, 856, 1069
BmiI	GGNNCC	7 cut(s)	512, 820, 841, 981, 1070, 1071, 1164
BmrFI	CCNGG	2 cut(s)	418, 1268
BmrI	ACTGGG	1 cut(s)	1179
BmsI	GCATC	2 cut(s)	756, 1231
BmuI	ACTGGG	1 cut(s)	1179
BoxI	GACNNNNGTC	1 cut(s)	1083
Bpu10I	CCTNAGC	1 cut(s)	1290
BpuMI	CCSGG	1 cut(s)	418
Bsa29I	ATCGAT	1 cut(s)	553
BsaAI	YACGTR	1 cut(s)	1277
BsaJI	CCNNGG	1 cut(s)	1074
BsaXI	ACNNNNNCTCC	4 cut(s)	444, 454, 474, 484
Bsc4I	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
Bse1I	ACTGG	4 cut(s)	521, 1003, 1066, 1185
BseBI	CCWGG	1 cut(s)	1268
BseCI	ATCGAT	1 cut(s)	553
BseDI	CCNNGG	1 cut(s)	1074
BseGI	GGATG	1 cut(s)	771
BseLI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
BseMII	CTCAG	4 cut(s)	239, 618, 1281, 1392
BseNI	ACTGG	4 cut(s)	521, 1003, 1066, 1185
BseRI	GAGGAG	2 cut(s)	468, 1166
BseSI	GKGCMC	2 cut(s)	842, 1210
BseXI	GCAGC	2 cut(s)	720, 798
BsgI	GTGCAG	1 cut(s)	376
BshFI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1090
BshNI	GGYRCC	2 cut(s)	839, 979
BshVI	ATCGAT	1 cut(s)	553
BsiSI	CCGG	1 cut(s)	417
BslFI	GGGAC	3 cut(s)	416, 433, 1082
BslI	CCNNNNNNNGG	6 cut(s)	379, 479, 835, 849, 850, 865
BsmAI	GTCTC	3 cut(s)	196, 1381, 1421
BsmFI	GGGAC	3 cut(s)	416, 433, 1082
BsnI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1090
Bsp1286I	GDGCHC	3 cut(s)	226, 842, 1210
Bsp143I	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
BspACI	CCGC	3 cut(s)	668, 730, 859
BspANI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1090
BspCNI	CTCAG	4 cut(s)	238, 619, 1282, 1391
BspDI	ATCGAT	1 cut(s)	553
BspHI	TCATGA	1 cut(s)	1237
BspLI	GGNNCC	7 cut(s)	512, 820, 841, 981, 1070, 1071, 1164
BspPI	GGATC	6 cut(s)	97, 540, 1157, 1170, 1320, 1413
BspQI	GCTCTTC	2 cut(s)	1010, 1138
BspT107I	GGYRCC	2 cut(s)	839, 979
BsrI	ACTGG	4 cut(s)	521, 1003, 1066, 1185
BssECI	CCNNGG	1 cut(s)	1074
BssMI	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
BssSI	CACGAG	1 cut(s)	1305
Bst2BI	CACGAG	1 cut(s)	1305
Bst2UI	CCWGG	1 cut(s)	1268
Bst4CI	ACNGT	3 cut(s)	265, 396, 424
Bst6I	CTCTTC	2 cut(s)	1010, 1138
BstBAI	YACGTR	1 cut(s)	1277
BstC8I	GCNNGC	4 cut(s)	666, 859, 1092, 1206
BstDEI	CTNAG	7 cut(s)	9, 225, 527, 627, 1223, 1290, 1378
BstDSI	CCRYGG	1 cut(s)	1074
BstF5I	GGATG	1 cut(s)	771
BstH2I	RGCGCY	1 cut(s)	893
BstHHI	GCGC	2 cut(s)	828, 892
BstKTI	GATC	8 cut(s)	72, 105, 241, 535, 1165, 1237, 1315, 1408
BstMAI	GTCTC	3 cut(s)	196, 1381, 1421
BstMBI	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
BstMWI	GCNNNNNNNGC	2 cut(s)	832, 1145
BstNI	CCWGG	1 cut(s)	1268
BstNSI	RCATGY	1 cut(s)	304
BstPAI	GACNNNNGTC	1 cut(s)	1083
BstSCI	CCNGG	2 cut(s)	416, 1266
BstSFI	CTRYAG	2 cut(s)	156, 1027
BstSLI	GKGCMC	2 cut(s)	842, 1210
BstSNI	TACGTA	1 cut(s)	1277
BstV1I	GCAGC	2 cut(s)	720, 798
BstX2I	RGATCY	3 cut(s)	532, 1162, 1405
BstYI	RGATCY	3 cut(s)	532, 1162, 1405
Bsu15I	ATCGAT	1 cut(s)	553
BsuRI	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1090
BsuTUI	ATCGAT	1 cut(s)	553
BtgI	CCRYGG	1 cut(s)	1074
BtsCI	GGATG	1 cut(s)	771
BtsI	GCAGTG	1 cut(s)	642
BtsIMutI	CAGTG	3 cut(s)	359, 514, 642
Cac8I	GCNNGC	4 cut(s)	666, 859, 1092, 1206
CaiI	CAGNNNCTG	1 cut(s)	1061
CciI	TCATGA	1 cut(s)	1237
CfoI	GCGC	2 cut(s)	828, 892
Cfr13I	GGNCC	4 cut(s)	511, 819, 856, 1069
ClaI	ATCGAT	1 cut(s)	553
Csp6I	GTAC	6 cut(s)	231, 266, 572, 879, 1188, 1274
CviQI	GTAC	6 cut(s)	231, 266, 572, 879, 1188, 1274
DdeI	CTNAG	7 cut(s)	9, 225, 527, 627, 1223, 1290, 1378
DpnI	GATC	8 cut(s)	71, 104, 240, 534, 1164, 1236, 1314, 1407
DpnII	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
DraI	TTTAAA	1 cut(s)	1200
Eam1104I	CTCTTC	2 cut(s)	1010, 1138
EarI	CTCTTC	2 cut(s)	1010, 1138
EciI	GGCGGA	1 cut(s)	683
Eco105I	TACGTA	1 cut(s)	1277
Eco24I	GRGCYC	1 cut(s)	226
Eco32I	GATATC	1 cut(s)	202
Eco47I	GGWCC	1 cut(s)	1069
Eco47III	AGCGCT	1 cut(s)	891
EcoO109I	RGGNCCY	1 cut(s)	1069
EcoRI	GAATTC	1 cut(s)	289
EcoRII	CCWGG	1 cut(s)	1266
EcoRV	GATATC	1 cut(s)	202
EcoT22I	ATGCAT	1 cut(s)	1441
EcoT38I	GRGCYC	1 cut(s)	226
FaqI	GGGAC	3 cut(s)	416, 433, 1082
FauI	CCCGC	1 cut(s)	866
FbaI	TGATCA	2 cut(s)	238, 1234
Fnu4HI	GCNGC	3 cut(s)	731, 734, 812
FokI	GGATG	1 cut(s)	778
FriOI	GRGCYC	1 cut(s)	226
Fsp4HI	GCNGC	3 cut(s)	731, 734, 812
FspBI	CTAG	2 cut(s)	147, 800
GlaI	GCGC	2 cut(s)	827, 891
GluI	GCNGC	3 cut(s)	731, 734, 812
HaeII	RGCGCY	1 cut(s)	893
HaeIII	GGCC	7 cut(s)	467, 513, 698, 705, 821, 857, 1090
HapII	CCGG	1 cut(s)	417
HhaI	GCGC	2 cut(s)	828, 892
Hin6I	GCGC	2 cut(s)	826, 890
HinP1I	GCGC	2 cut(s)	826, 890
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	7 cut(s)	349, 550, 721, 871, 1105, 1330, 1355
HpaII	CCGG	1 cut(s)	417
HphI	GGTGA	7 cut(s)	139, 233, 362, 590, 880, 1114, 1394
Hpy166II	GTNNAC	8 cut(s)	175, 233, 392, 522, 601, 760, 1078, 1190
Hpy188I	TCNGA	7 cut(s)	10, 102, 138, 228, 295, 960, 1335
Hpy188III	TCNNGA	5 cut(s)	147, 205, 1238, 1307, 1409
Hpy8I	GTNNAC	8 cut(s)	175, 233, 392, 522, 601, 760, 1078, 1190
HpyAV	CCTTC	5 cut(s)	401, 746, 859, 1311, 1357
HpyCH4III	ACNGT	3 cut(s)	265, 396, 424
HpyCH4IV	ACGT	3 cut(s)	708, 1192, 1276
HpyF10VI	GCNNNNNNNGC	2 cut(s)	832, 1145
HpyF3I	CTNAG	7 cut(s)	9, 225, 527, 627, 1223, 1290, 1378
HpySE526I	ACGT	3 cut(s)	708, 1192, 1276
HspAI	GCGC	2 cut(s)	826, 890
KflI	GGGWCCC	1 cut(s)	1069
Ksp22I	TGATCA	2 cut(s)	238, 1234
Kzo9I	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
LguI	GCTCTTC	2 cut(s)	1010, 1138
LmnI	GCTCC	1 cut(s)	419
Lsp1109I	GCAGC	2 cut(s)	720, 798
LweI	GCATC	2 cut(s)	756, 1231
MaeI	CTAG	2 cut(s)	147, 800
MaeII	ACGT	3 cut(s)	708, 1192, 1276
MaeIII	GTNAC	6 cut(s)	127, 332, 444, 460, 1057, 1382
MalI	GATC	8 cut(s)	71, 104, 240, 534, 1164, 1236, 1314, 1407
MboI	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
MboII	GAAGA	3 cut(s)	52, 1027, 1155
MflI	RGATCY	3 cut(s)	532, 1162, 1405
MhlI	GDGCHC	3 cut(s)	226, 842, 1210
MluCI	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1350, 1369
MlyI	GAGTC	2 cut(s)	358, 1324
Mph1103I	ATGCAT	1 cut(s)	1441
MroXI	GAANNNNTTC	1 cut(s)	756
MseI	TTAA	3 cut(s)	476, 1007, 1199
MslI	CAYNNNNRTG	2 cut(s)	866, 1225
MspI	CCGG	1 cut(s)	417
MspR9I	CCNGG	2 cut(s)	418, 1268
MvaI	CCWGG	1 cut(s)	1268
MwoI	GCNNNNNNNGC	2 cut(s)	832, 1145
NciI	CCSGG	1 cut(s)	418
NdeII	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
NlaIV	GGNNCC	7 cut(s)	512, 820, 841, 981, 1070, 1071, 1164
NmuCI	GTSAC	4 cut(s)	127, 332, 444, 1382
NsiI	ATGCAT	1 cut(s)	1441
NspI	RCATGY	1 cut(s)	304
OliI	CACNNNNGTG	1 cut(s)	1225
PagI	TCATGA	1 cut(s)	1237
PciI	ACATGT	1 cut(s)	300
PciSI	GCTCTTC	2 cut(s)	1010, 1138
PcsI	WCGNNNNNNNCGW	2 cut(s)	912, 1312
PdmI	GAANNNNTTC	1 cut(s)	756
PfeI	GAWTC	5 cut(s)	550, 721, 871, 1105, 1355
PfoI	TCCNGGA	1 cut(s)	416
PkrI	GCNGC	3 cut(s)	732, 735, 813
PleI	GAGTC	2 cut(s)	357, 1324
PpsI	GAGTC	2 cut(s)	357, 1324
Ppu21I	YACGTR	1 cut(s)	1277
PpuMI	RGGWCCY	1 cut(s)	1069
PscI	ACATGT	1 cut(s)	300
PshAI	GACNNNNGTC	1 cut(s)	1083
Psp5II	RGGWCCY	1 cut(s)	1069
Psp6I	CCWGG	1 cut(s)	1266
PspGI	CCWGG	1 cut(s)	1266
PspN4I	GGNNCC	7 cut(s)	512, 820, 841, 981, 1070, 1071, 1164
PspPI	GGNCC	4 cut(s)	511, 819, 856, 1069
PspPPI	RGGWCCY	1 cut(s)	1069
PstNI	CAGNNNCTG	1 cut(s)	1061
PsuI	RGATCY	3 cut(s)	532, 1162, 1405
RsaI	GTAC	6 cut(s)	232, 267, 573, 880, 1189, 1275
RsaNI	GTAC	6 cut(s)	231, 266, 572, 879, 1188, 1274
RseI	CAYNNNNRTG	2 cut(s)	866, 1225
SapI	GCTCTTC	2 cut(s)	1010, 1138
SaqAI	TTAA	3 cut(s)	476, 1007, 1199
SatI	GCNGC	3 cut(s)	731, 734, 812
Sau3AI	GATC	8 cut(s)	69, 102, 238, 532, 1162, 1234, 1312, 1405
Sau96I	GGNCC	4 cut(s)	511, 819, 856, 1069
SchI	GAGTC	2 cut(s)	358, 1324
ScrFI	CCNGG	2 cut(s)	418, 1268
SduI	GDGCHC	3 cut(s)	226, 842, 1210
SfaNI	GCATC	2 cut(s)	756, 1231
SfcI	CTRYAG	2 cut(s)	156, 1027
SinI	GGWCC	1 cut(s)	1069
SmiMI	CAYNNNNRTG	2 cut(s)	866, 1225
SnaBI	TACGTA	1 cut(s)	1277
Sse9I	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1350, 1369
SsiI	CCGC	3 cut(s)	668, 730, 859
SspMI	CTAG	2 cut(s)	147, 800
StyD4I	CCNGG	2 cut(s)	416, 1266
TaaI	ACNGT	3 cut(s)	265, 396, 424
TaiI	ACGT	3 cut(s)	711, 1195, 1279
TaqI	TCGA	3 cut(s)	553, 915, 1315
TasI	AATT	8 cut(s)	29, 250, 289, 477, 539, 677, 1350, 1369
TatI	WGTACW	4 cut(s)	230, 265, 571, 1187
TauI	GCSGC	1 cut(s)	733
TfiI	GAWTC	5 cut(s)	550, 721, 871, 1105, 1355
Tru1I	TTAA	3 cut(s)	476, 1007, 1199
Tru9I	TTAA	3 cut(s)	476, 1007, 1199
TscAI	CASTG	3 cut(s)	359, 521, 642
TseFI	GTSAC	4 cut(s)	127, 332, 444, 1382
TseI	GCWGC	2 cut(s)	733, 811
Tsp45I	GTSAC	4 cut(s)	127, 332, 444, 1382
TspDTI	ATGAA	2 cut(s)	792, 878
TspRI	CASTG	3 cut(s)	359, 521, 642
VpaK11BI	GGWCC	1 cut(s)	1069
XapI	RAATTY	3 cut(s)	289, 677, 1369
XbaI	TCTAGA	1 cut(s)	146
XceI	RCATGY	1 cut(s)	304
XmnI	GAANNNNTTC	1 cut(s)	756
XspI	CTAG	2 cut(s)	147, 800
Zsp2I	ATGCAT	1 cut(s)	1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.