MD17G1146900.v1.1

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009557
Physical Location & Seq
Reverse (-)
13297888 .. 13301293
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1146900.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCTATGGAAGAAGAGGTAGTATTCAAGTTGCTATGTCATGTTGATAAGGTTGGTAGTCTGATAGGGAAGGGTGGCTTTGTTATTCGGACTATGCAAAATGAAACTGGTGCATCAATTAAGATAGCTGATTGTGCACCTGATTCAGATGAGCGAGTTATTATCATATCTGCACAAGAGGAAGCTGTTATACGCGCACATCATCGAATTGCGGAGATTGGGTTTGAACCTGGTGCTGCAGTGGTAGGTTTCATTATTACAGAAACGAGAAGAGCTACAGGTGCTTGTACACGGATTTTTCCAAAGGAAAAAGTTCAGAAGGGTAGCTACCAAAATGATGAAGTTGTACAGGTTATTGGAAGCTTGCCGTCTGTACAGGATGCACTCTTTCAGATAACAAGTAGGATTCGGGAAACAATTTTCCCTATGAGACCTCCTTATGCTAATTTCAGTGGCCCGCCCTATATGTCTCCATTTCCTGAGATGCCTCCCCACTCCTTTAGACCGAGGCATAACCCAGCCTCCCCTGGTCCCTATCCTTCACCTGGATTCCATCATGGAGTTGATCATTCTGCTTTTCCATCACAAACTCCAGGTCATCAGCCTTCATTTTCTCATGGTATGGATCACAACGGTCCAGCTCACATGGACCGAGTTCCCTATTCTTTTGGTAATGAGCGACCAGGACATGGTCCAACATTTGAGAGTCCTTCTCCAAGAGTTGAGATGACGATTCCTCAGACGTTGATAACTCACGATTGTGGAGAGAACAACAGCAATCTGAACCAGATCCGGCAGATCTCAGGCGCGCATCTGATTGTTCACGATTCAAAACCTGGATCGTTGGAAACCCTAGTTGTCGTGTCCGGACACAACCTTGACAATCTGTTGACCTGTTCTAGCAGCACCCGTTTGAATGAAGAATGGACGCGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

313

Amino Acids

34.19

Weight (kDa)

6.17

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 9 - 71 3.5e-14 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 689
AccII CGCG 3 cut(s) 195, 809, 931
AccIII TCCGGA 1 cut(s) 866
AciI CCGC 2 cut(s) 212, 458
AclWI GGATC 3 cut(s) 633, 784, 847
AfaI GTAC 3 cut(s) 289, 348, 375
AfiI CCNNNNNNNGG 2 cut(s) 545, 689
AflIII ACRYGT 1 cut(s) 929
AgsI TTSAA 4 cut(s) 28, 227, 831, 916
AjnI CCWGG 6 cut(s) 229, 526, 544, 592, 682, 835
AleI CACNNNNGTG 1 cut(s) 759
AluBI AGCT 6 cut(s) 128, 185, 275, 327, 363, 641
AluI AGCT 6 cut(s) 128, 185, 275, 327, 363, 641
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 424, 474
Alw44I GTGCAC 1 cut(s) 135
AlwI GGATC 3 cut(s) 633, 784, 847
Aor13HI TCCGGA 1 cut(s) 866
AoxI GGCC 1 cut(s) 454
ApaLI GTGCAC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 236, 903
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 3 cut(s) 197, 809, 811
AspS9I GGNCC 5 cut(s) 455, 530, 635, 649, 692
AsuHPI GGTGA 1 cut(s) 534
AvaII GGWCC 4 cut(s) 530, 635, 649, 692
BaeGI GKGCMC 1 cut(s) 139
BarI GAAGNNNNNNTAC 4 cut(s) 174, 206, 311, 343
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 223, 915
BccI CCATC 2 cut(s) 561, 589
BceAI ACGGC 1 cut(s) 352
BciT130I CCWGG 6 cut(s) 231, 528, 546, 594, 684, 837
BclI TGATCA 1 cut(s) 565
BcoDI GTCTC 2 cut(s) 424, 474
BfaI CTAG 2 cut(s) 854, 900
BfmI CTRYAG 2 cut(s) 237, 276
BglII AGATCT 1 cut(s) 798
BisI GCNGC 2 cut(s) 237, 904
BlsI GCNGC 2 cut(s) 238, 905
Bme1390I CCNGG 6 cut(s) 231, 528, 546, 594, 684, 837
Bme18I GGWCC 4 cut(s) 530, 635, 649, 692
BmgT120I GGNCC 5 cut(s) 455, 530, 635, 649, 692
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 6 cut(s) 231, 528, 546, 594, 684, 837
BmsI GCATC 4 cut(s) 122, 370, 474, 820
BplI GAGNNNNNCTC 2 cut(s) 697, 729
BpmI CTGGAG 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 2 cut(s) 506, 526
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 506, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 689
Bse1I ACTGG 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 866
BseBI CCWGG 6 cut(s) 231, 528, 546, 594, 684, 837
BseDI CCNNGG 2 cut(s) 506, 526
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 545, 689
BseMII CTCAG 3 cut(s) 471, 752, 816
BseNI ACTGG 1 cut(s) 112
BsePI GCGCGC 1 cut(s) 807
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 2 cut(s) 223, 915
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 156
Bsh1236I CGCG 3 cut(s) 195, 809, 931
BshFI GGCC 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 2 cut(s) 793, 867
BslFI GGGAC 1 cut(s) 516
BslI CCNNNNNNNGG 2 cut(s) 545, 689
BsmAI GTCTC 2 cut(s) 424, 474
BsmFI GGGAC 1 cut(s) 516
BsnI GGCC 1 cut(s) 456
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 139
Bsp13I TCCGGA 1 cut(s) 866
Bsp1407I TGTACA 3 cut(s) 287, 346, 373
Bsp143I GATC 5 cut(s) 565, 625, 789, 798, 839
BspACI CCGC 2 cut(s) 212, 458
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 3 cut(s) 472, 751, 815
BspEI TCCGGA 1 cut(s) 866
BspFNI CGCG 3 cut(s) 195, 809, 931
BspLI GGNNCC 1 cut(s) 532
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 3 cut(s) 633, 784, 847
BspQI GCTCTTC 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 424
BsrGI TGTACA 3 cut(s) 287, 346, 373
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 506, 526
BssHII GCGCGC 1 cut(s) 807
BssMI GATC 5 cut(s) 565, 625, 789, 798, 839
Bst2UI CCWGG 6 cut(s) 231, 528, 546, 594, 684, 837
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 2 cut(s) 9, 265
BstAUI TGTACA 3 cut(s) 287, 346, 373
BstC8I GCNNGC 3 cut(s) 365, 458, 809
BstDEI CTNAG 3 cut(s) 480, 738, 802
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 3 cut(s) 195, 809, 931
BstHHI GCGC 3 cut(s) 197, 809, 811
BstKTI GATC 5 cut(s) 568, 628, 792, 801, 842
BstMAI GTCTC 2 cut(s) 424, 474
BstMBI GATC 5 cut(s) 565, 625, 789, 798, 839
BstMWI GCNNNNNNNGC 2 cut(s) 134, 281
BstNI CCWGG 6 cut(s) 231, 528, 546, 594, 684, 837
BstSCI CCNGG 6 cut(s) 229, 526, 544, 592, 682, 835
BstSFI CTRYAG 2 cut(s) 237, 276
BstSLI GKGCMC 1 cut(s) 139
BstUI CGCG 3 cut(s) 195, 809, 931
BstV1I GCAGC 2 cut(s) 223, 915
BstX2I RGATCY 2 cut(s) 789, 798
BstYI RGATCY 2 cut(s) 789, 798
BsuRI GGCC 1 cut(s) 456
BtsCI GGATG 1 cut(s) 385
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 457
Cac8I GCNNGC 3 cut(s) 365, 458, 809
CfoI GCGC 3 cut(s) 197, 809, 811
Cfr13I GGNCC 5 cut(s) 455, 530, 635, 649, 692
CsiI ACCWGGT 1 cut(s) 229
Csp6I GTAC 3 cut(s) 288, 347, 374
CviAII CATG 5 cut(s) 41, 557, 617, 646, 689
CviQI GTAC 3 cut(s) 288, 347, 374
DdeI CTNAG 3 cut(s) 480, 738, 802
DpnI GATC 5 cut(s) 567, 627, 791, 800, 841
DpnII GATC 5 cut(s) 565, 625, 789, 798, 839
Eam1104I CTCTTC 2 cut(s) 9, 265
EarI CTCTTC 2 cut(s) 9, 265
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 4 cut(s) 530, 635, 649, 692
EcoRII CCWGG 6 cut(s) 229, 526, 544, 592, 682, 835
FaeI CATG 5 cut(s) 44, 560, 620, 649, 692
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 516
FatI CATG 5 cut(s) 40, 556, 616, 645, 688
FauI CCCGC 1 cut(s) 465
FbaI TGATCA 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 237, 904
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 237, 904
FspBI CTAG 2 cut(s) 854, 900
GlaI GCGC 3 cut(s) 196, 808, 810
GluI GCNGC 2 cut(s) 237, 904
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 576
HaeIII GGCC 1 cut(s) 456
HapII CCGG 2 cut(s) 793, 867
HhaI GCGC 3 cut(s) 197, 809, 811
Hin1II CATG 5 cut(s) 44, 560, 620, 649, 692
Hin6I GCGC 3 cut(s) 195, 807, 809
HinP1I GCGC 3 cut(s) 195, 807, 809
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 6 cut(s) 143, 406, 549, 706, 733, 827
HpaII CCGG 2 cut(s) 793, 867
HphI GGTGA 1 cut(s) 534
Hpy166II GTNNAC 4 cut(s) 137, 290, 823, 891
Hpy188I TCNGA 8 cut(s) 63, 90, 148, 318, 393, 741, 783, 816
Hpy188III TCNNGA 5 cut(s) 410, 479, 755, 824, 867
Hpy8I GTNNAC 4 cut(s) 137, 290, 823, 891
HpyAV CCTTC 5 cut(s) 64, 313, 549, 615, 720
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 6 cut(s) 97, 113, 137, 173, 239, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 281
HpyF3I CTNAG 3 cut(s) 480, 738, 802
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 5 cut(s) 44, 560, 620, 649, 692
HspAI GCGC 3 cut(s) 195, 807, 809
Kpn2I TCCGGA 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 5 cut(s) 565, 625, 789, 798, 839
LguI GCTCTTC 1 cut(s) 265
Lsp1109I GCAGC 2 cut(s) 223, 915
LweI GCATC 4 cut(s) 122, 370, 474, 820
MabI ACCWGGT 1 cut(s) 229
MaeI CTAG 2 cut(s) 854, 900
MaeII ACGT 1 cut(s) 743
MalI GATC 5 cut(s) 567, 627, 791, 800, 841
MboI GATC 5 cut(s) 565, 625, 789, 798, 839
MboII GAAGA 4 cut(s) 23, 26, 282, 932
MflI RGATCY 2 cut(s) 789, 798
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 4 cut(s) 117, 207, 417, 445
MluI ACGCGT 1 cut(s) 929
MlyI GAGTC 1 cut(s) 715
MmeI TCCRAC 2 cut(s) 719, 825
MnlI CCTC 7 cut(s) 10, 172, 444, 498, 501, 532, 747
MroI TCCGGA 1 cut(s) 866
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 1 cut(s) 759
MspI CCGG 2 cut(s) 793, 867
MspR9I CCNGG 6 cut(s) 231, 528, 546, 594, 684, 837
MvaI CCWGG 6 cut(s) 231, 528, 546, 594, 684, 837
MvnI CGCG 3 cut(s) 195, 809, 931
MwoI GCNNNNNNNGC 2 cut(s) 134, 281
NdeII GATC 5 cut(s) 565, 625, 789, 798, 839
NlaIII CATG 5 cut(s) 44, 560, 620, 649, 692
NlaIV GGNNCC 1 cut(s) 532
OliI CACNNNNGTG 1 cut(s) 759
PauI GCGCGC 1 cut(s) 807
PciSI GCTCTTC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 5 cut(s) 143, 406, 549, 733, 827
PflFI GACNNNGTC 1 cut(s) 690
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 2 cut(s) 238, 905
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
Psp6I CCWGG 6 cut(s) 229, 526, 544, 592, 682, 835
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 6 cut(s) 229, 526, 544, 592, 682, 835
PspN4I GGNNCC 1 cut(s) 532
PspPI GGNCC 5 cut(s) 455, 530, 635, 649, 692
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 789, 798
PsyI GACNNNGTC 1 cut(s) 690
PteI GCGCGC 1 cut(s) 807
RsaI GTAC 3 cut(s) 289, 348, 375
RsaNI GTAC 3 cut(s) 288, 347, 374
RseI CAYNNNNRTG 1 cut(s) 759
SapI GCTCTTC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 237, 904
Sau3AI GATC 5 cut(s) 565, 625, 789, 798, 839
Sau96I GGNCC 5 cut(s) 455, 530, 635, 649, 692
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 6 cut(s) 231, 528, 546, 594, 684, 837
SduI GDGCHC 1 cut(s) 139
SexAI ACCWGGT 1 cut(s) 229
SfaNI GCATC 4 cut(s) 122, 370, 474, 820
SfcI CTRYAG 2 cut(s) 237, 276
SinI GGWCC 4 cut(s) 530, 635, 649, 692
SmiMI CAYNNNNRTG 1 cut(s) 759
Sse9I AATT 4 cut(s) 117, 207, 417, 445
SsiI CCGC 2 cut(s) 212, 458
SspMI CTAG 2 cut(s) 854, 900
StyD4I CCNGG 6 cut(s) 229, 526, 544, 592, 682, 835
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 1 cut(s) 746
TaqI TCGA 1 cut(s) 205
TaqII GACCGA 2 cut(s) 520, 666
TasI AATT 4 cut(s) 117, 207, 417, 445
TatI WGTACW 3 cut(s) 287, 346, 373
TfiI GAWTC 5 cut(s) 143, 406, 549, 733, 827
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 2 cut(s) 246, 457
TseI GCWGC 2 cut(s) 236, 903
TspDTI ATGAA 5 cut(s) 117, 241, 354, 597, 933
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 2 cut(s) 246, 457
Tth111I GACNNNGTC 1 cut(s) 690
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 135
VpaK11BI GGWCC 4 cut(s) 530, 635, 649, 692
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 854, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.