Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0009557

Physical Location & Seq

Genomic Coordinates Forward (+)

69417156 .. 69421320

Gene Structure 4165 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG475400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGATCCATGGATCTTTAATAAGGATAACAGGAGACTTTTCAGCTGTGAAGAAAGCACTTCTGTCTGTTTCTGGTTGCCTTCTGGATAATGCTAAGGTAGATGCAGCCAACTCTAGCGCTACCAAAGTCGCCAACTCTAGCGCTACCAAAGTTTCTGGGGGGTCGATCCATGGAACTGGTATGCCTGCACAAGTTGACTCTGTTCCTCATCGGGGTTTTGTGTCTAGCTTTAATGCTGTAGATTATCAGTCCAGGAGTTACTCTTCTAATTCTGCACCAGATAACAGCAGTCAACGAATGGCTATGGAAGAAGAGGTGGTATTTAAGTTGTTATGCCAACATGATAAGGTTGGTAGTCTGATAGGGAAAGGTGGCTCCATTATACGAACTTTTCAAAGTGAAACAGGTGCATCTATCAAGATTGTTGATGCTGCACCTGATTCGGATGAGCGGGTTGTTGTCATATCTGCACAAGAGAATCCTGAGCAAAATTATTCCCCCGCACAAGAAGCTGTTATGCGTGTGCATCATCGAATTGCGGAGATTGGATTTGAACCTGGTGCTGCAGTAGTGGCTAGGCTCCTCCTACATCCACAGCAGATAGGTTGTTTATTGGGTAAAGGAGGTTTTATTATTAATGAAATGAGGCGAGCTACTGGTGCTAGTATTAGGATTTTTCCAAAAGAACAAGTTCAGAAATCTGCTTACCACCATGATGACGTTGTACAGGTTATTGGAAGCTTGCAGTCTGTACAGGATGCACTCTTTCATATCACAAGTAGGATTCGTGAAGCCATATTCCCAATGAAACAATCATATCCAAATTTTGGTGCCCCACCGTACATGTCTTCATTTCCTGAGATGCCTCCCCCCTCATTTAGACCGAGGCATAATTCAACCTCCCCTGGTGCGTATCCTTCACCTGTTGGATTCCATCATGGAATGGATCATTCTGCTTTTCGATCCCAACCTCCAGACCACCAGTCCCCATTTTCACATGGGGATCACAGTGGTCTGTCTCACATGGACCGAGTTCCGTATTCTTATGGTGGTGAGCGTCCGGGACATGGTCCGGCATTTGAGAGATCTTCTCCAAGAGCGTGGACTCCCCAGGGAGTAAGCTCTGGAAACCTAAGGGAAATTGGAGATGCTAATTTGGGCTTGGCTCCAAGGAGTGGGTCTCTACGAAGCGGCTGTCAACCACCTCACTTAACCAGCACAACCATCGAGATCATGATTCCTCAAGCATTCATGACTCACGTTTATGGAGAGAACAACAGTAATCTGAATCAGATTCGGCAGATATCAGGTGCAAATTTAGTTGTTCATGATTCAAAACCCGGATCCTTGGAAACCCAAGTTGTAGTATCCGGATCCCCAGATAAAACACATGCTGCTCAGAGCCTTATCCATGGTTTCATCCTTTGTGGTCAGACACAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

481

Amino Acids

51.65

Weight (kDa)

7.4

Isoelectric Point (pI)

50.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	108 - 159	9.3e-14	KH domain
KH_1	PF00013	193 - 259	1.8e-11	KH domain
KH_1	PF00013	408 - 471	4.6e-10	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51580
fragaria_vesca	FvH4_6g37150 FvH4_6g37150 FvH4_6g37150
malus_domestica	MD09G1161100.v1.1 MD17G1146900.v1.1
prunus_persica	Prupe.3G020000_v2.0.a1 Prupe.3G020000_v2.0.a1
pyrus_communis	pycom09g07950 pycom17g13960
rosa_chinensis	RchiOBHm_Chr2g0149721
rosa_laevigata	RLG00000020442
rosa_multiflora	Rmu_sc0001091.1_g000008
rosa_roxburghii	Rroxscaffold_2G00097760
rosa_rugosa	Rorug02G0415600 Rorug02G0415700 Rorug02G0415800
rosa_samantha	Rh2AG475400 Rh2BG486800 Rh2CG461000 Rh2DG496000
rosa_wichuraiana	Rw2G038740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	830
AccB7I	CCANNNNNTGG	2 cut(s)	827, 1175
AccBSI	CCGCTC	1 cut(s)	451
AccIII	TCCGGA	1 cut(s)	1370
AciI	CCGC	4 cut(s)	451, 501, 539, 1191
AclWI	GGATC	9 cut(s)	19, 160, 954, 957, 1011, 1338, 1351, 1368, 1381
AcsI	RAATTY	2 cut(s)	823, 1315
AfaI	GTAC	3 cut(s)	726, 753, 842
AfeI	AGCGCT	2 cut(s)	118, 142
AfiI	CCNNNNNNNGG	4 cut(s)	212, 827, 1067, 1175
AflIII	ACRYGT	1 cut(s)	843
AgsI	TTSAA	4 cut(s)	395, 554, 897, 1335
AhdI	GACNNNNNGTC	1 cut(s)	982
AjnI	CCWGG	4 cut(s)	251, 556, 904, 1110
AluBI	AGCT	6 cut(s)	44, 228, 512, 653, 741, 1122
AluI	AGCT	6 cut(s)	44, 228, 512, 653, 741, 1122
Alw26I	GTCTC	3 cut(s)	27, 1023, 1185
AlwI	GGATC	9 cut(s)	19, 160, 954, 957, 1011, 1338, 1351, 1368, 1381
Aor13HI	TCCGGA	1 cut(s)	1370
Aor51HI	AGCGCT	2 cut(s)	118, 142
ApeKI	GCWGC	4 cut(s)	104, 431, 563, 1394
ApoI	RAATTY	2 cut(s)	823, 1315
ArsI	GACNNNNNNTTYG	2 cut(s)	1180, 1212
AseI	ATTAAT	1 cut(s)	636
Asp700I	GAANNNNTTC	1 cut(s)	690
AspLEI	GCGC	2 cut(s)	119, 143
AspS9I	GGNCC	2 cut(s)	1027, 1070
AsuC2I	CCSGG	2 cut(s)	1062, 1341
AsuHPI	GGTGA	2 cut(s)	912, 1064
AvaII	GGWCC	2 cut(s)	1027, 1070
AxyI	CCTNAGG	1 cut(s)	1133
BaeGI	GKGCMC	1 cut(s)	835
BamHI	GGATCC	2 cut(s)	1343, 1373
BanI	GGYRCC	1 cut(s)	830
BbsI	GAAGAC	1 cut(s)	840
BbvI	GCAGC	4 cut(s)	116, 418, 550, 1381
BccI	CCATC	2 cut(s)	942, 1232
BcgI	CGANNNNNNTGC	4 cut(s)	423, 457, 1207, 1241
BciT130I	CCWGG	4 cut(s)	253, 558, 906, 1112
BciVI	GTATCC	2 cut(s)	924, 1378
BcnI	CCSGG	2 cut(s)	1062, 1341
BcoDI	GTCTC	3 cut(s)	27, 1023, 1185
BfaI	CTAG	5 cut(s)	114, 138, 225, 576, 663
BfmI	CTRYAG	2 cut(s)	237, 564
BfoI	RGCGCY	2 cut(s)	120, 144
BfuI	GTATCC	2 cut(s)	924, 1378
BglII	AGATCT	1 cut(s)	1085
BisI	GCNGC	5 cut(s)	105, 432, 564, 1192, 1395
BlsI	GCNGC	5 cut(s)	106, 433, 565, 1193, 1396
Bme1390I	CCNGG	6 cut(s)	253, 558, 906, 1062, 1112, 1341
Bme18I	GGWCC	2 cut(s)	1027, 1070
BmeRI	GACNNNNNGTC	1 cut(s)	982
BmgT120I	GGNCC	2 cut(s)	1027, 1070
BmiI	GGNNCC	6 cut(s)	376, 581, 832, 1167, 1345, 1375
BmrFI	CCNGG	6 cut(s)	253, 558, 906, 1062, 1112, 1341
BmsI	GCATC	7 cut(s)	91, 418, 419, 535, 748, 852, 1138
BpiI	GAAGAC	1 cut(s)	840
BplI	GAGNNNNNCTC	4 cut(s)	1075, 1107, 1165, 1197
BpmI	CTGGAG	1 cut(s)	957
Bpu10I	CCTNAGC	2 cut(s)	93, 483
BpuEI	CTTGAG	1 cut(s)	1227
BpuMI	CCSGG	2 cut(s)	1062, 1341
BsaI	GGTCTC	1 cut(s)	1185
BsaJI	CCNNGG	9 cut(s)	7, 169, 884, 904, 1110, 1111, 1169, 1347, 1411
BsaWI	WCCGGW	1 cut(s)	1370
Bsc4I	CCNNNNNNNGG	4 cut(s)	212, 827, 1067, 1175
Bse1I	ACTGG	3 cut(s)	181, 661, 982
Bse21I	CCTNAGG	1 cut(s)	1133
BseAI	TCCGGA	1 cut(s)	1370
BseBI	CCWGG	4 cut(s)	253, 558, 906, 1112
BseDI	CCNNGG	9 cut(s)	7, 169, 884, 904, 1110, 1111, 1169, 1347, 1411
BseGI	GGATG	4 cut(s)	451, 589, 763, 1419
BseLI	CCNNNNNNNGG	4 cut(s)	212, 827, 1067, 1175
BseMII	CTCAG	3 cut(s)	474, 849, 1412
BseNI	ACTGG	3 cut(s)	181, 661, 982
BseRI	GAGGAG	1 cut(s)	572
BseSI	GKGCMC	1 cut(s)	835
BseXI	GCAGC	4 cut(s)	116, 418, 550, 1381
BsgI	GTGCAG	4 cut(s)	171, 258, 417, 453
BshNI	GGYRCC	1 cut(s)	830
BsiSI	CCGG	4 cut(s)	1061, 1073, 1341, 1371
BslFI	GGGAC	2 cut(s)	970, 1077
BslI	CCNNNNNNNGG	4 cut(s)	212, 827, 1067, 1175
BsmAI	GTCTC	3 cut(s)	27, 1023, 1185
BsmFI	GGGAC	2 cut(s)	970, 1077
BsmI	GAATGC	1 cut(s)	1247
Bso31I	GGTCTC	1 cut(s)	1185
Bsp1286I	GDGCHC	1 cut(s)	835
Bsp13I	TCCGGA	1 cut(s)	1370
Bsp1407I	TGTACA	2 cut(s)	724, 751
Bsp19I	CCATGG	3 cut(s)	7, 169, 1411
BspACI	CCGC	4 cut(s)	451, 501, 539, 1191
BspCNI	CTCAG	3 cut(s)	475, 850, 1411
BspEI	TCCGGA	1 cut(s)	1370
BspHI	TCATGA	3 cut(s)	1233, 1251, 1327
BspLI	GGNNCC	6 cut(s)	376, 581, 832, 1167, 1345, 1375
BspMAI	CTGCAG	1 cut(s)	568
BspPI	GGATC	9 cut(s)	19, 160, 954, 957, 1011, 1338, 1351, 1368, 1381
BspT107I	GGYRCC	1 cut(s)	830
BspTNI	GGTCTC	1 cut(s)	1185
BsrBI	CCGCTC	1 cut(s)	451
BsrGI	TGTACA	2 cut(s)	724, 751
BsrI	ACTGG	3 cut(s)	181, 661, 982
BssECI	CCNNGG	9 cut(s)	7, 169, 884, 904, 1110, 1111, 1169, 1347, 1411
BssT1I	CCWWGG	5 cut(s)	7, 169, 1169, 1347, 1411
Bst2UI	CCWGG	4 cut(s)	253, 558, 906, 1112
Bst4CI	ACNGT	3 cut(s)	840, 1010, 1280
Bst6I	CTCTTC	2 cut(s)	268, 306
BstAUI	TGTACA	2 cut(s)	724, 751
BstC8I	GCNNGC	3 cut(s)	186, 651, 743
BstDEI	CTNAG	5 cut(s)	93, 483, 858, 1133, 1398
BstDSI	CCRYGG	3 cut(s)	7, 169, 1411
BstF5I	GGATG	4 cut(s)	451, 589, 763, 1419
BstH2I	RGCGCY	2 cut(s)	120, 144
BstHHI	GCGC	2 cut(s)	119, 143
BstMAI	GTCTC	3 cut(s)	27, 1023, 1185
BstMWI	GCNNNNNNNGC	3 cut(s)	509, 572, 659
BstNI	CCWGG	4 cut(s)	253, 558, 906, 1112
BstNSI	RCATGY	2 cut(s)	847, 1394
BstSCI	CCNGG	6 cut(s)	251, 556, 904, 1060, 1110, 1339
BstSFI	CTRYAG	2 cut(s)	237, 564
BstSLI	GKGCMC	1 cut(s)	835
BstV1I	GCAGC	4 cut(s)	116, 418, 550, 1381
BstV2I	GAAGAC	1 cut(s)	840
BstX2I	RGATCY	4 cut(s)	11, 1085, 1343, 1373
BstXI	CCANNNNNNTGG	2 cut(s)	176, 1101
BstYI	RGATCY	4 cut(s)	11, 1085, 1343, 1373
Bsu36I	CCTNAGG	1 cut(s)	1133
BsuI	GTATCC	2 cut(s)	924, 1378
BtgI	CCRYGG	3 cut(s)	7, 169, 1411
BtsCI	GGATG	4 cut(s)	451, 589, 763, 1419
BtsIMutI	CAGTG	1 cut(s)	1015
Cac8I	GCNNGC	3 cut(s)	186, 651, 743
CciI	TCATGA	3 cut(s)	1233, 1251, 1327
CfoI	GCGC	2 cut(s)	119, 143
Cfr13I	GGNCC	2 cut(s)	1027, 1070
CseI	GACGC	1 cut(s)	1046
CsiI	ACCWGGT	1 cut(s)	556
Csp6I	GTAC	3 cut(s)	725, 752, 841
CviQI	GTAC	3 cut(s)	725, 752, 841
DdeI	CTNAG	5 cut(s)	93, 483, 858, 1133, 1398
DriI	GACNNNNNGTC	1 cut(s)	982
Eam1104I	CTCTTC	2 cut(s)	268, 306
Eam1105I	GACNNNNNGTC	1 cut(s)	982
EarI	CTCTTC	2 cut(s)	268, 306
Eco130I	CCWWGG	5 cut(s)	7, 169, 1169, 1347, 1411
Eco31I	GGTCTC	1 cut(s)	1185
Eco32I	GATATC	1 cut(s)	1305
Eco47I	GGWCC	2 cut(s)	1027, 1070
Eco47III	AGCGCT	2 cut(s)	118, 142
Eco81I	CCTNAGG	1 cut(s)	1133
EcoRII	CCWGG	4 cut(s)	251, 556, 904, 1110
EcoRV	GATATC	1 cut(s)	1305
EcoT14I	CCWWGG	5 cut(s)	7, 169, 1169, 1347, 1411
ErhI	CCWWGG	5 cut(s)	7, 169, 1169, 1347, 1411
FaqI	GGGAC	2 cut(s)	970, 1077
FauI	CCCGC	2 cut(s)	444, 508
Fnu4HI	GCNGC	5 cut(s)	105, 432, 564, 1192, 1395
FokI	GGATG	4 cut(s)	458, 576, 770, 1406
Fsp4HI	GCNGC	5 cut(s)	105, 432, 564, 1192, 1395
FspBI	CTAG	5 cut(s)	114, 138, 225, 576, 663
GlaI	GCGC	2 cut(s)	118, 142
GluI	GCNGC	5 cut(s)	105, 432, 564, 1192, 1395
GsuI	CTGGAG	1 cut(s)	957
HaeII	RGCGCY	2 cut(s)	120, 144
HapII	CCGG	4 cut(s)	1061, 1073, 1341, 1371
HgaI	GACGC	1 cut(s)	1046
HhaI	GCGC	2 cut(s)	119, 143
Hin6I	GCGC	2 cut(s)	117, 141
HinP1I	GCGC	2 cut(s)	117, 141
HincII	GTYRAC	3 cut(s)	196, 293, 1199
HindII	GTYRAC	3 cut(s)	196, 293, 1199
HindIII	AAGCTT	1 cut(s)	739
HpaII	CCGG	4 cut(s)	1061, 1073, 1341, 1371
HphI	GGTGA	2 cut(s)	912, 1064
Hpy166II	GTNNAC	4 cut(s)	196, 293, 1104, 1199
Hpy188I	TCNGA	7 cut(s)	360, 445, 696, 1287, 1293, 1401, 1434
Hpy8I	GTNNAC	4 cut(s)	196, 293, 1104, 1199
HpyAV	CCTTC	2 cut(s)	89, 927
HpyCH4III	ACNGT	3 cut(s)	840, 1010, 1280
HpyCH4IV	ACGT	2 cut(s)	720, 1260
HpyF10VI	GCNNNNNNNGC	3 cut(s)	509, 572, 659
HpyF3I	CTNAG	5 cut(s)	93, 483, 858, 1133, 1398
HpySE526I	ACGT	2 cut(s)	720, 1260
HspAI	GCGC	2 cut(s)	117, 141
Kpn2I	TCCGGA	1 cut(s)	1370
LmnI	GCTCC	3 cut(s)	380, 585, 1171
Lsp1109I	GCAGC	4 cut(s)	116, 418, 550, 1381
LweI	GCATC	7 cut(s)	91, 418, 419, 535, 748, 852, 1138
MabI	ACCWGGT	1 cut(s)	556
MaeI	CTAG	5 cut(s)	114, 138, 225, 576, 663
MaeII	ACGT	2 cut(s)	720, 1260
MaeIII	GTNAC	1 cut(s)	257
MbiI	CCGCTC	1 cut(s)	451
MboII	GAAGA	6 cut(s)	61, 255, 320, 323, 840, 1080
MflI	RGATCY	4 cut(s)	11, 1085, 1343, 1373
MhlI	GDGCHC	1 cut(s)	835
MluCI	AATT	8 cut(s)	268, 490, 534, 823, 892, 1140, 1153, 1315
MlyI	GAGTC	3 cut(s)	191, 1099, 1249
MmeI	TCCRAC	1 cut(s)	907
MroI	TCCGGA	1 cut(s)	1370
MroXI	GAANNNNTTC	1 cut(s)	690
MseI	TTAA	5 cut(s)	17, 231, 324, 636, 1211
MslI	CAYNNNNRTG	2 cut(s)	714, 1263
MspA1I	CMGCKG	1 cut(s)	44
MspI	CCGG	4 cut(s)	1061, 1073, 1341, 1371
MspR9I	CCNGG	6 cut(s)	253, 558, 906, 1062, 1112, 1341
Mva1269I	GAATGC	1 cut(s)	1247
MvaI	CCWGG	4 cut(s)	253, 558, 906, 1112
MwoI	GCNNNNNNNGC	3 cut(s)	509, 572, 659
NciI	CCSGG	2 cut(s)	1062, 1341
NcoI	CCATGG	3 cut(s)	7, 169, 1411
NlaIV	GGNNCC	6 cut(s)	376, 581, 832, 1167, 1345, 1375
NspI	RCATGY	2 cut(s)	847, 1394
PagI	TCATGA	3 cut(s)	1233, 1251, 1327
PasI	CCCWGGG	1 cut(s)	1111
PciI	ACATGT	1 cut(s)	843
PctI	GAATGC	1 cut(s)	1247
PdmI	GAANNNNTTC	1 cut(s)	690
PfeI	GAWTC	8 cut(s)	440, 478, 784, 930, 1237, 1288, 1294, 1331
PflFI	GACNNNGTC	1 cut(s)	1068
PflMI	CCANNNNNTGG	2 cut(s)	827, 1175
PfoI	TCCNGGA	2 cut(s)	251, 1060
PkrI	GCNGC	5 cut(s)	106, 433, 565, 1193, 1396
PleI	GAGTC	3 cut(s)	191, 1099, 1249
PpsI	GAGTC	3 cut(s)	191, 1099, 1249
PscI	ACATGT	1 cut(s)	843
PshBI	ATTAAT	1 cut(s)	636
Psp6I	CCWGG	4 cut(s)	251, 556, 904, 1110
PspGI	CCWGG	4 cut(s)	251, 556, 904, 1110
PspN4I	GGNNCC	6 cut(s)	376, 581, 832, 1167, 1345, 1375
PspPI	GGNCC	2 cut(s)	1027, 1070
PstI	CTGCAG	1 cut(s)	568
PsuI	RGATCY	4 cut(s)	11, 1085, 1343, 1373
PsyI	GACNNNGTC	1 cut(s)	1068
PvuII	CAGCTG	1 cut(s)	44
RsaI	GTAC	3 cut(s)	726, 753, 842
RsaNI	GTAC	3 cut(s)	725, 752, 841
RseI	CAYNNNNRTG	2 cut(s)	714, 1263
SaqAI	TTAA	5 cut(s)	17, 231, 324, 636, 1211
SatI	GCNGC	5 cut(s)	105, 432, 564, 1192, 1395
Sau96I	GGNCC	2 cut(s)	1027, 1070
SchI	GAGTC	3 cut(s)	191, 1099, 1249
ScrFI	CCNGG	6 cut(s)	253, 558, 906, 1062, 1112, 1341
SduI	GDGCHC	1 cut(s)	835
SexAI	ACCWGGT	1 cut(s)	556
SfaNI	GCATC	7 cut(s)	91, 418, 419, 535, 748, 852, 1138
SfcI	CTRYAG	2 cut(s)	237, 564
SinI	GGWCC	2 cut(s)	1027, 1070
SmiMI	CAYNNNNRTG	2 cut(s)	714, 1263
SmlI	CTYRAG	1 cut(s)	1242
SmoI	CTYRAG	1 cut(s)	1242
Sse9I	AATT	8 cut(s)	268, 490, 534, 823, 892, 1140, 1153, 1315
SsiI	CCGC	4 cut(s)	451, 501, 539, 1191
SspMI	CTAG	5 cut(s)	114, 138, 225, 576, 663
StyD4I	CCNGG	6 cut(s)	251, 556, 904, 1060, 1110, 1339
StyI	CCWWGG	5 cut(s)	7, 169, 1169, 1347, 1411
TaaI	ACNGT	3 cut(s)	840, 1010, 1280
TaiI	ACGT	2 cut(s)	723, 1263
TaqI	TCGA	4 cut(s)	164, 532, 961, 1227
TaqII	GACCGA	2 cut(s)	898, 1044
TasI	AATT	8 cut(s)	268, 490, 534, 823, 892, 1140, 1153, 1315
TatI	WGTACW	2 cut(s)	724, 751
TauI	GCSGC	1 cut(s)	1194
TfiI	GAWTC	8 cut(s)	440, 478, 784, 930, 1237, 1288, 1294, 1331
Tru1I	TTAA	5 cut(s)	17, 231, 324, 636, 1211
Tru9I	TTAA	5 cut(s)	17, 231, 324, 636, 1211
TscAI	CASTG	1 cut(s)	1015
TseI	GCWGC	4 cut(s)	104, 431, 563, 1394
TspDTI	ATGAA	7 cut(s)	654, 758, 821, 840, 1240, 1316, 1408
TspGWI	ACGGA	1 cut(s)	1026
TspRI	CASTG	1 cut(s)	1015
Tth111I	GACNNNGTC	1 cut(s)	1068
Van91I	CCANNNNNTGG	2 cut(s)	827, 1175
VpaK11BI	GGWCC	2 cut(s)	1027, 1070
VspI	ATTAAT	1 cut(s)	636
XapI	RAATTY	2 cut(s)	823, 1315
XceI	RCATGY	2 cut(s)	847, 1394
XmnI	GAANNNNTTC	1 cut(s)	690
XspI	CTAG	5 cut(s)	114, 138, 225, 576, 663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.