Rorug02G0415700

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009557
Physical Location & Seq
Forward (+)
53203847 .. 53205172
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0415700.1

Sequence Viewer

Length: 1326 bp
ATGACGTCCATTGACGAAGTTACTGCAAGCTTCGCGGCCTCTCTCGCCCTAGCGGGCTCTGATGTCGTTGACATCTCGCACTTGAACGGCTCGGACAGGCGTTCCAACTCTCAGGCTTTCCTTCTAGCCAAACCAATGACAAAGAAACAGTTTGGCACTCAGGATCTGCAGCGTCACTTCCGACGCATCTGGATCATTGATGGAGCCTTCAAGGTTCACGACAGTACTCAAGGCCGCTTTGTTTTTTCCTTTGATCTGAAACGTGATCGAAATAAGGTTTTACGGGGAGGGCCGTGGTATTTTAACAAAGCTCCGGTTTTGCTACAGCATTATGATGGCATGGAGGATCCTCTTGTCATCCCAATGAATCTGCTCTTTTTTTGGGTCAAAATCACAGGTATCCCTCCAGCCCTAGAAGAGAAAACGATTATCATGAATGTTGCTTCTGTTGCGGGTAAGTATTTGGATTTTGATCAAAGACTGTATAACAATAAGAATCAAATTAGGGTTCATGTAGCTCATGCTATTTCCCATCCTTTCTTCTTGAAACGGACTCTCAAGATCATCCCTGGAGTGGTGAAAGAGATTACCTTTTTTTATGAAAATTTGCATGGAATATGCAAAGATTGCCAACTCCTGTGTCATGAACAGGACGTTTGCCCTACTGTTGATGCCCAAGCTATGCATAACACCACTACCCGATCGGAGACATCAGGGAAGTCGCTCACATCCCATTTTCCAGGTTTGTCTGATCTTCGTTTCCAGAATGGATCGTTCCGCTTTCAAGGCCTGCAAACACCGCTACTGCCTCCAGTAGTGCGTAACCTATTTGGCACTTCTGCTATAAACAAGCATCGCAAGCCTATTGTTGTGCGTAGTGAAAACCTAAGGCCTGCTGCGGAAGGAAATGCCCTAGCCCTAGTTCCTGTGGACGTGGAACCATCCACTGTGAAAAGAAATAGGCCTGCATCACCATATACGTCCCCAAAGAAACTGAAATTTCTGTTTCATGATGGTGAGACTGCTGCTAGCTCCGCTATGCTGCCTAAGAAACTGAAAGTTCCAGAATTCCACACCAAATTCTCTGCTAAATCACTGGGTCTCATTGCAGCCCCGGGGGGTGTCTTAGTTCCTAAGGAAGGATCACTCCCTAAGGAGTCTCTGCAGCTCGTCAAAGTGGAATCAAAGAAGAAAAGAGGCCGGCCTTTGGGCTCGAAAAACAAGAACTCTTCAAAGGGTAAGAAGATTGCAACTGACGAGGTGCTTAGCATGCTTTGCCCTGGAAAACCTCCTAGCCCTAGTGTTAAGGGCAAGGAAAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

441

Amino Acids

48.97

Weight (kDa)

9.9

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 38 - 116 5.1e-13 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccII CGCG 1 cut(s) 35
AciI CCGC 8 cut(s) 35, 53, 235, 452, 778, 800, 899, 1035
AclWI GGATC 6 cut(s) 171, 200, 341, 354, 778, 1150
AcsI RAATTY 4 cut(s) 604, 998, 1067, 1079
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 226
AfiI CCNNNNNNNGG 3 cut(s) 574, 1139, 1207
AgsI TTSAA 5 cut(s) 85, 211, 547, 785, 1233
AjiI CACGTC 1 cut(s) 934
AjnI CCWGG 3 cut(s) 568, 739, 1279
AluBI AGCT 6 cut(s) 30, 311, 518, 680, 1032, 1168
AluI AGCT 6 cut(s) 30, 311, 518, 680, 1032, 1168
Alw26I GTCTC 4 cut(s) 701, 1013, 1106, 1164
AlwI GGATC 6 cut(s) 171, 200, 341, 354, 778, 1150
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 1114
AoxI GGCC 8 cut(s) 36, 232, 290, 787, 890, 962, 1198, 1202
ApeKI GCWGC 6 cut(s) 169, 896, 1025, 1042, 1109, 1165
ApoI RAATTY 4 cut(s) 604, 998, 1067, 1079
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 2 cut(s) 1115, 1116
AsuHPI GGTGA 3 cut(s) 589, 963, 1028
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 1114
AxyI CCTNAGG 3 cut(s) 887, 1134, 1152
BamHI GGATCC 1 cut(s) 346
BanII GRGCYC 2 cut(s) 59, 1214
BbvI GCAGC 6 cut(s) 181, 883, 1012, 1029, 1121, 1177
BccI CCATC 5 cut(s) 194, 329, 540, 949, 1007
BceAI ACGGC 2 cut(s) 103, 277
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 3 cut(s) 570, 741, 1281
BciVI GTATCC 1 cut(s) 410
BclI TGATCA 1 cut(s) 472
BcnI CCSGG 2 cut(s) 1115, 1116
BcoDI GTCTC 4 cut(s) 701, 1013, 1106, 1164
BfaI CTAG 8 cut(s) 50, 125, 413, 914, 920, 1029, 1293, 1299
BfmI CTRYAG 3 cut(s) 167, 323, 1163
BfuI GTATCC 1 cut(s) 410
BisI GCNGC 8 cut(s) 36, 170, 235, 897, 1026, 1043, 1110, 1166
BlpI GCTNAGC 1 cut(s) 1265
BlsI GCNGC 8 cut(s) 37, 171, 236, 898, 1027, 1044, 1111, 1167
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 5 cut(s) 570, 741, 1115, 1116, 1281
BmeT110I CYCGRG 1 cut(s) 1114
BmgBI CACGTC 1 cut(s) 934
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 3 cut(s) 205, 348, 939
BmrFI CCNGG 5 cut(s) 570, 741, 1115, 1116, 1281
BmrI ACTGGG 1 cut(s) 1106
BmsI GCATC 4 cut(s) 195, 661, 862, 977
BmtI GCTAGC 1 cut(s) 1032
BmuI ACTGGG 1 cut(s) 1106
BpmI CTGGAG 3 cut(s) 390, 591, 795
Bpu1102I GCTNAGC 1 cut(s) 1265
BpuEI CTTGAG 2 cut(s) 213, 542
BpuMI CCSGG 2 cut(s) 1115, 1116
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1106
BsaJI CCNNGG 6 cut(s) 293, 568, 1113, 1114, 1115, 1279
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 1139, 1207
Bse118I RCCGGY 1 cut(s) 1200
Bse1I ACTGG 2 cut(s) 812, 1101
Bse21I CCTNAGG 3 cut(s) 887, 1134, 1152
Bse3DI GCAATG 1 cut(s) 1104
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 3 cut(s) 570, 741, 1281
BseDI CCNNGG 6 cut(s) 293, 568, 1113, 1114, 1115, 1279
BseGI GGATG 5 cut(s) 357, 532, 564, 728, 941
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 3 cut(s) 574, 1139, 1207
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 2 cut(s) 125, 173
BseNI ACTGG 2 cut(s) 812, 1101
BseXI GCAGC 6 cut(s) 181, 883, 1012, 1029, 1121, 1177
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 704
BshFI GGCC 8 cut(s) 38, 234, 292, 789, 892, 964, 1200, 1204
BsiEI CGRYCG 1 cut(s) 704
BsiHKCI CYCGRG 1 cut(s) 1114
BsiSI CCGG 3 cut(s) 314, 1115, 1201
BslFI GGGAC 1 cut(s) 967
BslI CCNNNNNNNGG 3 cut(s) 574, 1139, 1207
BsmAI GTCTC 4 cut(s) 701, 1013, 1106, 1164
BsmFI GGGAC 1 cut(s) 967
BsnI GGCC 8 cut(s) 38, 234, 292, 789, 892, 964, 1200, 1204
Bso31I GGTCTC 1 cut(s) 1106
BsoBI CYCGRG 1 cut(s) 1114
Bsp1286I GDGCHC 2 cut(s) 59, 1214
Bsp1720I GCTNAGC 1 cut(s) 1265
BspACI CCGC 8 cut(s) 35, 53, 235, 452, 778, 800, 899, 1035
BspANI GGCC 8 cut(s) 38, 234, 292, 789, 892, 964, 1200, 1204
BspCNI CTCAG 2 cut(s) 124, 172
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 3 cut(s) 432, 643, 1009
BspLI GGNNCC 3 cut(s) 205, 348, 939
BspMAI CTGCAG 2 cut(s) 171, 1167
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 6 cut(s) 171, 200, 341, 354, 778, 1150
BspTNI GGTCTC 1 cut(s) 1106
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 1 cut(s) 1200
BsrI ACTGG 2 cut(s) 812, 1101
BssAI RCCGGY 1 cut(s) 1200
BssECI CCNNGG 6 cut(s) 293, 568, 1113, 1114, 1115, 1279
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 570, 741, 1281
Bst4CI ACNGT 5 cut(s) 150, 224, 483, 667, 949
Bst6I CTCTTC 2 cut(s) 411, 1234
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 627, 1275
BstC8I GCNNGC 9 cut(s) 28, 55, 791, 860, 894, 966, 1030, 1202, 1271
BstDEI CTNAG 8 cut(s) 111, 159, 887, 1047, 1126, 1134, 1152, 1265
BstDSI CCRYGG 1 cut(s) 293
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 5 cut(s) 357, 532, 564, 728, 941
BstFNI CGCG 1 cut(s) 35
BstMAI GTCTC 4 cut(s) 701, 1013, 1106, 1164
BstMCI CGRYCG 1 cut(s) 704
BstNI CCWGG 3 cut(s) 570, 741, 1281
BstNSI RCATGY 1 cut(s) 1273
BstSCI CCNGG 5 cut(s) 568, 739, 1113, 1114, 1279
BstSFI CTRYAG 3 cut(s) 167, 323, 1163
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 6 cut(s) 181, 883, 1012, 1029, 1121, 1177
BstX2I RGATCY 2 cut(s) 163, 346
BstYI RGATCY 2 cut(s) 163, 346
Bsu36I CCTNAGG 3 cut(s) 887, 1134, 1152
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 8 cut(s) 38, 234, 292, 789, 892, 964, 1200, 1204
BtgI CCRYGG 1 cut(s) 293
BtgZI GCGATG 1 cut(s) 839
BtrI CACGTC 1 cut(s) 934
BtsCI GGATG 5 cut(s) 357, 532, 564, 728, 941
BtsIMutI CAGTG 2 cut(s) 945, 1094
Cac8I GCNNGC 9 cut(s) 28, 55, 791, 860, 894, 966, 1030, 1202, 1271
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 3 cut(s) 432, 643, 1009
Cfr10I RCCGGY 1 cut(s) 1200
Cfr13I GGNCC 1 cut(s) 290
Cfr9I CCCGGG 1 cut(s) 1114
CseI GACGC 2 cut(s) 161, 192
Csp6I GTAC 1 cut(s) 225
CviAII CATG 8 cut(s) 340, 433, 512, 521, 611, 644, 1010, 1270
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 8 cut(s) 111, 159, 887, 1047, 1126, 1134, 1152, 1265
Eam1104I CTCTTC 2 cut(s) 411, 1234
EarI CTCTTC 2 cut(s) 411, 1234
Eco147I AGGCCT 3 cut(s) 789, 892, 964
Eco24I GRGCYC 2 cut(s) 59, 1214
Eco31I GGTCTC 1 cut(s) 1106
Eco81I CCTNAGG 3 cut(s) 887, 1134, 1152
Eco88I CYCGRG 1 cut(s) 1114
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoRI GAATTC 1 cut(s) 1067
EcoRII CCWGG 3 cut(s) 568, 739, 1279
EcoT22I ATGCAT 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 59, 1214
FaeI CATG 8 cut(s) 343, 436, 515, 524, 614, 647, 1013, 1273
FaqI GGGAC 1 cut(s) 967
FatI CATG 8 cut(s) 339, 432, 511, 520, 610, 643, 1009, 1269
FauI CCCGC 2 cut(s) 46, 445
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 8 cut(s) 36, 170, 235, 897, 1026, 1043, 1110, 1166
FokI GGATG 5 cut(s) 344, 519, 551, 715, 928
FriOI GRGCYC 2 cut(s) 59, 1214
FseI GGCCGGCC 1 cut(s) 1204
Fsp4HI GCNGC 8 cut(s) 36, 170, 235, 897, 1026, 1043, 1110, 1166
FspBI CTAG 8 cut(s) 50, 125, 413, 914, 920, 1029, 1293, 1299
GluI GCNGC 8 cut(s) 36, 170, 235, 897, 1026, 1043, 1110, 1166
GsuI CTGGAG 3 cut(s) 390, 591, 795
HaeIII GGCC 8 cut(s) 38, 234, 292, 789, 892, 964, 1200, 1204
HapII CCGG 3 cut(s) 314, 1115, 1201
HgaI GACGC 2 cut(s) 161, 192
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 343, 436, 515, 524, 614, 647, 1013, 1273
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 5 cut(s) 367, 496, 553, 1157, 1181
HpaII CCGG 3 cut(s) 314, 1115, 1201
HphI GGTGA 3 cut(s) 589, 963, 1028
Hpy166II GTNNAC 3 cut(s) 70, 217, 931
Hpy188I TCNGA 6 cut(s) 61, 94, 182, 258, 706, 751
Hpy8I GTNNAC 3 cut(s) 70, 217, 931
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 4 cut(s) 131, 217, 896, 1133
HpyCH4III ACNGT 5 cut(s) 150, 224, 483, 667, 949
HpyCH4IV ACGT 5 cut(s) 5, 262, 654, 933, 980
HpyF3I CTNAG 8 cut(s) 111, 159, 887, 1047, 1126, 1134, 1152, 1265
HpySE526I ACGT 5 cut(s) 5, 262, 654, 933, 980
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 343, 436, 515, 524, 614, 647, 1013, 1273
KroI GCCGGC 1 cut(s) 1200
KroNI GCCGGC 1 cut(s) 1202
Ksp22I TGATCA 1 cut(s) 472
LmnI GCTCC 3 cut(s) 203, 316, 1037
Lsp1109I GCAGC 6 cut(s) 181, 883, 1012, 1029, 1121, 1177
LweI GCATC 4 cut(s) 195, 661, 862, 977
MaeI CTAG 8 cut(s) 50, 125, 413, 914, 920, 1029, 1293, 1299
MaeII ACGT 5 cut(s) 5, 262, 654, 933, 980
MaeIII GTNAC 3 cut(s) 19, 173, 821
MboII GAAGA 6 cut(s) 428, 532, 746, 1201, 1221, 1255
MflI RGATCY 2 cut(s) 163, 346
MhlI GDGCHC 2 cut(s) 59, 1214
MluCI AATT 5 cut(s) 501, 604, 998, 1067, 1079
MlyI GAGTC 2 cut(s) 547, 1166
MmeI TCCRAC 2 cut(s) 129, 205
MnlI CCTC 9 cut(s) 49, 281, 337, 360, 414, 819, 1190, 1252, 1299
Mph1103I ATGCAT 1 cut(s) 687
MroNI GCCGGC 1 cut(s) 1200
MseI TTAA 2 cut(s) 303, 1305
MslI CAYNNNNRTG 3 cut(s) 333, 362, 1014
MspI CCGG 3 cut(s) 314, 1115, 1201
MspR9I CCNGG 5 cut(s) 570, 741, 1115, 1116, 1281
MvaI CCWGG 3 cut(s) 570, 741, 1281
MvnI CGCG 1 cut(s) 35
NaeI GCCGGC 1 cut(s) 1202
NciI CCSGG 2 cut(s) 1115, 1116
NgoMIV GCCGGC 1 cut(s) 1200
NheI GCTAGC 1 cut(s) 1028
NlaIII CATG 8 cut(s) 343, 436, 515, 524, 614, 647, 1013, 1273
NlaIV GGNNCC 3 cut(s) 205, 348, 939
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 1 cut(s) 1273
PaeI GCATGC 1 cut(s) 1273
PagI TCATGA 3 cut(s) 432, 643, 1009
PceI AGGCCT 3 cut(s) 789, 892, 964
PdiI GCCGGC 1 cut(s) 1202
PfeI GAWTC 3 cut(s) 367, 496, 1181
PkrI GCNGC 8 cut(s) 37, 171, 236, 898, 1027, 1044, 1111, 1167
Ple19I CGATCG 1 cut(s) 704
PleI GAGTC 2 cut(s) 547, 1165
PpsI GAGTC 2 cut(s) 547, 1165
Psp6I CCWGG 3 cut(s) 568, 739, 1279
PspGI CCWGG 3 cut(s) 568, 739, 1279
PspN4I GGNNCC 3 cut(s) 205, 348, 939
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 2 cut(s) 171, 1167
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 2 cut(s) 163, 346
PvuI CGATCG 1 cut(s) 704
RigI GGCCGGCC 1 cut(s) 1204
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
RseI CAYNNNNRTG 3 cut(s) 333, 362, 1014
SaqAI TTAA 2 cut(s) 303, 1305
SatI GCNGC 8 cut(s) 36, 170, 235, 897, 1026, 1043, 1110, 1166
Sau96I GGNCC 1 cut(s) 290
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 2 cut(s) 547, 1166
ScrFI CCNGG 5 cut(s) 570, 741, 1115, 1116, 1281
SduI GDGCHC 2 cut(s) 59, 1214
SfaNI GCATC 4 cut(s) 195, 661, 862, 977
SfcI CTRYAG 3 cut(s) 167, 323, 1163
SmaI CCCGGG 1 cut(s) 1116
SmiMI CAYNNNNRTG 3 cut(s) 333, 362, 1014
SmlI CTYRAG 2 cut(s) 228, 557
SmoI CTYRAG 2 cut(s) 228, 557
SphI GCATGC 1 cut(s) 1273
Sse9I AATT 5 cut(s) 501, 604, 998, 1067, 1079
SseBI AGGCCT 3 cut(s) 789, 892, 964
SsiI CCGC 8 cut(s) 35, 53, 235, 452, 778, 800, 899, 1035
SspMI CTAG 8 cut(s) 50, 125, 413, 914, 920, 1029, 1293, 1299
StuI AGGCCT 3 cut(s) 789, 892, 964
StyD4I CCNGG 5 cut(s) 568, 739, 1113, 1114, 1279
TaaI ACNGT 5 cut(s) 150, 224, 483, 667, 949
TaiI ACGT 5 cut(s) 8, 265, 657, 936, 983
TaqI TCGA 2 cut(s) 268, 1214
TasI AATT 5 cut(s) 501, 604, 998, 1067, 1079
TatI WGTACW 1 cut(s) 224
TauI GCSGC 2 cut(s) 38, 237
TfiI GAWTC 3 cut(s) 367, 496, 1181
Tru1I TTAA 2 cut(s) 303, 1305
Tru9I TTAA 2 cut(s) 303, 1305
TscAI CASTG 2 cut(s) 952, 1101
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 6 cut(s) 169, 896, 1025, 1042, 1109, 1165
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 6 cut(s) 380, 449, 500, 615, 660, 998
TspGWI ACGGA 1 cut(s) 565
TspMI CCCGGG 1 cut(s) 1114
TspRI CASTG 2 cut(s) 952, 1101
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 4 cut(s) 604, 998, 1067, 1079
XceI RCATGY 1 cut(s) 1273
XmaI CCCGGG 1 cut(s) 1114
XspI CTAG 8 cut(s) 50, 125, 413, 914, 920, 1029, 1293, 1299
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 226
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.