Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006867

Physical Location & Seq

Genomic Coordinates Reverse (-)

22674612 .. 22680752

Gene Structure 6141 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1190300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGCGGTCGACCGCGCCATTTAATCTCCTCAACCAACAATTTTTGAGCACACTCTGCTCCTCGTCCTACCCACCTTCTCTCAGACGCACCCACGCTTTGCTCTGGTCGTCGCACTCTCCGCACTTCTCCTCATTGCAATCTGGTTCATCCATGGAGGAGTCAAAGGTAGTTAATCAAGGGTCTGCTACGCGTGATAGCATTGCTATTCGATCTGATCACAAGAGTTACAGTTACAACCAACTAATCTCATCTGCATCAAGGATATCCAGATTGTTATCTACCGGTGAATTGAATGATACTTCTTCAGCATCGATTGATGGTGGGAAAGGACAAGAGAACCTTGGAGGGGCTAGAATAGGAATTGTGGCTAAGCCCTCAGCTGAGTTTGTTGCCGGTATACTTGGGACATGGCTAAGTGGAGGTGTTGCAGTTCCACTTGCTCTCAGCTATCCTGAAGTGGAGCTCTTGCATGTCATAAATGACTCAGATGTCTCAATGATACTGAGTACTGAAGAGCACATGCAACTCATGGAAAATGTTGCTGCTAAATGTGCTGCCCAAACTTCTCTTATTCCACCAGTTCCCAGTAGTTCTTCACCAGAAAGTGGAGCTGGTTGTTTACAAGCAGGAGGAATAGATGCAGATGCAATTTTTCACCGGAATATAAAACAATCAAGTGAGGATCCTGCATTAGTCATATACACTAGTGGCACAACGGGAAAACCGAAGGGAGTTGTTCACACACACAAAAGCATTAGTGCACAAGTCCAAACCTTGGCAGAAGCTTGGGGGTATACATTTGCTGATCAATTTTTGAATTGTCTACCTCTACATCATATCCTTCAATTTTTGTGCATTCGCTTGCAAAATTGTAAAGTTTTCAAATGCAGCTTACTTTTAGCTAATGTCTTCAGTGTTGAGTTTATGCCAAAATTTAGCGTGAGGGGTATTTGGCAGAGGTGGCGAGAAGCATATCCAATTAATGGATCTAATGCAGATGATGGCATAACGGTCTTTACTGGAGTTCCAACCATGTATGCTCGATTGATACAAGCTCATGAAGCAATGGATCCAGAGCTAAAAGCCACCTCTGCCAATGCCGCAAAACAGTTGCGTCTAATGATGTGTGGTTCCTCTGCACTCCCTCTTCCTGTAATGCAACAATGGGAAACCATCACAGGACATCGTCTTTTGGAACGATATGGCATGACAGAGTTTGTCATGGCAATATCAAACCCCTTAAGAGGTGAGCGGAAAGCAGGCACGGTTGGAAAACCATTTCCGGGTGTGGAGGTAAAGATTGTTGCTGAAGATGAAAGTGGAAGTGATACTACAGGCGTGGGTGAGCTGTGTGTCAAAAGTCCGTCATTATTTAAGGAATATTGGAAGCTTCCTGAGGTAACAAAGCAATCATTTACAAGTGATGGATACTTCAGAACTGGAGATGCTGGTACAGTGGACGAAGATGGGTACTACATCATATTAGGACGTACCAGTGCTGATATCATGAAGGTTGGTGGATACAAGTTATCTGCGTTAGAAATTGAATCAGTTCTTTTACAGCACCCAGCTGTGGAGGAGTGTTGTGTGTTGGGTTTACCAGACAGAGACTATGGAGAAGTTGTTTGTGCAATAATTGTGCCAGGGGCGGAAACAAAGAGGAAGCAGAAGGAAGAACTGAAACCTGCTATAAGCTTGGAGGAACTATCCTCTTGGGCCAAAGACAAACTTGCACCATACAAGATACCAACGCGACTGATTCTGTGGGATTCACTACCTCGGAATGCTATGGGAAAGGTGAACAAGAAAGAGTTGAAGAAAACTCTGGCTCCTGACCAATCGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009987 GO:0016020 GO:0016053 GO:0016054 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0019752 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043649 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046395 GO:0071704 GO:0072330 GO:0090409 GO:0090410 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00280 map00280

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

616

Amino Acids

66.9

Weight (kDa)

6.42

Isoelectric Point (pI)

42.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	116 - 285	7.6e-34	AMP-binding enzyme
AMP-binding	PF00501	335 - 462	5.4e-27	AMP-binding enzyme
AMP-binding_C	PF13193	514 - 599	3.7e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006867)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16170
fragaria_vesca	FvH4_6g31860 FvH4_6g31860 FvH4_6g31860
malus_domestica	MD09G1208800.v1.1 MD17G1190300.v1.1
prunus_persica	Prupe.3G065200_v2.0.a1 Prupe.3G065200_v2.0.a1
pyrus_communis	pycom09g12490 pycom17g19780
rosa_chinensis	RchiOBHm_Chr2g0139171 RchiOBHm_Chr2g0139181
rosa_laevigata	RLG00000019793
rosa_multiflora	Rmu_sc0001803.1_g000009 Rmu_sc0012067.1_g000001 Rmu_sc0037230.1_g000001
rosa_roxburghii	Rroxscaffold_2G00105570
rosa_rugosa	Rorug02G0355400
rosa_samantha	Rh2AG405400 Rh2AG405500 Rh2BG415900 Rh2CG391500 Rh2CG391600 Rh2DG425200 Rh2DG425300
rosa_wichuraiana	Rw2G033120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	488
Acc36I	ACCTGC	1 cut(s)	1693
AccB7I	CCANNNNNTGG	3 cut(s)	605, 775, 983
AccBSI	CCGCTC	1 cut(s)	1252
AccI	GTMKAC	4 cut(s)	8, 397, 794, 823
AccII	CGCG	3 cut(s)	14, 190, 1753
AciI	CCGC	6 cut(s)	4, 12, 119, 1101, 1252, 1649
AclWI	GGATC	5 cut(s)	677, 690, 994, 1064, 1077
AcsI	RAATTY	1 cut(s)	932
AcuI	CTGAAG	6 cut(s)	288, 474, 531, 895, 1329, 1417
AfaI	GTAC	4 cut(s)	508, 1453, 1472, 1492
AfiI	CCNNNNNNNGG	7 cut(s)	346, 605, 775, 983, 1244, 1283, 1573
AflII	CTTAAG	1 cut(s)	1240
AflIII	ACRYGT	1 cut(s)	188
AgeI	ACCGGT	1 cut(s)	281
AgsI	TTSAA	6 cut(s)	292, 817, 845, 883, 1547, 1816
AhlI	ACTAGT	1 cut(s)	704
AjnI	CCWGG	1 cut(s)	1642
Alw21I	GWGCWC	4 cut(s)	50, 465, 519, 763
Alw26I	GTCTC	2 cut(s)	496, 1602
Alw44I	GTGCAC	1 cut(s)	759
AlwI	GGATC	5 cut(s)	677, 690, 994, 1064, 1077
AoxI	GGCC	1 cut(s)	1716
ApaLI	GTGCAC	1 cut(s)	759
ApeKI	GCWGC	3 cut(s)	542, 554, 888
ApoI	RAATTY	1 cut(s)	932
AseI	ATTAAT	1 cut(s)	981
AsiGI	ACCGGT	1 cut(s)	281
Asp700I	GAANNNNTTC	1 cut(s)	1551
AspLEI	GCGC	1 cut(s)	16
AspS9I	GGNCC	1 cut(s)	1716
AsuC2I	CCSGG	1 cut(s)	1284
AsuHPI	GGTGA	6 cut(s)	296, 588, 647, 1259, 1355, 1810
AxyI	CCTNAGG	1 cut(s)	1395
BaeGI	GKGCMC	1 cut(s)	763
BamHI	GGATCC	2 cut(s)	682, 1069
BanII	GRGCYC	1 cut(s)	465
BarI	GAAGNNNNNNTAC	2 cut(s)	1315, 1347
BbsI	GAAGAC	1 cut(s)	901
Bbv12I	GWGCWC	4 cut(s)	50, 465, 519, 763
BbvCI	CCTCAGC	1 cut(s)	376
BbvI	GCAGC	3 cut(s)	529, 541, 900
BccI	CCATC	5 cut(s)	311, 995, 1181, 1418, 1460
BciT130I	CCWGG	1 cut(s)	1644
BciVI	GTATCC	2 cut(s)	1421, 1514
BclI	TGATCA	2 cut(s)	214, 805
BcnI	CCSGG	1 cut(s)	1284
BcoDI	GTCTC	2 cut(s)	496, 1602
BcuI	ACTAGT	1 cut(s)	704
BfaI	CTAG	2 cut(s)	351, 705
BfmI	CTRYAG	1 cut(s)	1332
BfrI	CTTAAG	1 cut(s)	1240
BfuAI	ACCTGC	1 cut(s)	1693
BfuI	GTATCC	2 cut(s)	1421, 1514
BisI	GCNGC	4 cut(s)	543, 555, 889, 1101
BlpI	GCTNAGC	1 cut(s)	369
BlsI	GCNGC	4 cut(s)	544, 556, 890, 1102
BmcAI	AGTACT	1 cut(s)	508
Bme1390I	CCNGG	2 cut(s)	1284, 1644
BmgT120I	GGNCC	1 cut(s)	1716
BmiI	GGNNCC	4 cut(s)	684, 1071, 1132, 1830
BmrFI	CCNGG	2 cut(s)	1284, 1644
BmrI	ACTGGG	1 cut(s)	579
BmsI	GCATC	5 cut(s)	263, 317, 628, 634, 1435
BmuI	ACTGGG	1 cut(s)	579
BpiI	GAAGAC	1 cut(s)	901
BpmI	CTGGAG	2 cut(s)	1041, 1461
Bpu10I	CCTNAGC	1 cut(s)	376
Bpu1102I	GCTNAGC	1 cut(s)	369
BpuMI	CCSGG	1 cut(s)	1284
Bsa29I	ATCGAT	1 cut(s)	311
BsaBI	GATNNNNATC	1 cut(s)	274
BsaJI	CCNNGG	5 cut(s)	150, 340, 774, 1643, 1778
BsaWI	WCCGGW	2 cut(s)	281, 657
BsaXI	ACNNNNNCTCC	4 cut(s)	1608, 1638, 1813, 1843
Bsc4I	CCNNNNNNNGG	7 cut(s)	346, 605, 775, 983, 1244, 1283, 1573
Bse118I	RCCGGY	2 cut(s)	281, 392
Bse1I	ACTGG	5 cut(s)	578, 585, 1024, 1444, 1494
Bse21I	CCTNAGG	1 cut(s)	1395
Bse3DI	GCAATG	3 cut(s)	131, 198, 1071
Bse8I	GATNNNNATC	1 cut(s)	274
BseBI	CCWGG	1 cut(s)	1644
BseCI	ATCGAT	1 cut(s)	311
BseDI	CCNNGG	5 cut(s)	150, 340, 774, 1643, 1778
BseGI	GGATG	1 cut(s)	146
BseJI	GATNNNNATC	1 cut(s)	274
BseLI	CCNNNNNNNGG	7 cut(s)	346, 605, 775, 983, 1244, 1283, 1573
BseMI	GCAATG	3 cut(s)	131, 198, 1071
BseMII	CTCAG	7 cut(s)	94, 372, 390, 457, 494, 498, 1386
BseNI	ACTGG	5 cut(s)	578, 585, 1024, 1444, 1494
BseRI	GAGGAG	5 cut(s)	17, 49, 118, 170, 1592
BseSI	GKGCMC	1 cut(s)	763
BseXI	GCAGC	3 cut(s)	529, 541, 900
BseYI	CCCAGC	1 cut(s)	1567
BsgI	GTGCAG	1 cut(s)	1122
Bsh1236I	CGCG	3 cut(s)	14, 190, 1753
Bsh1285I	CGRYCG	2 cut(s)	8, 12
BshFI	GGCC	1 cut(s)	1718
BshTI	ACCGGT	1 cut(s)	281
BshVI	ATCGAT	1 cut(s)	311
BsiEI	CGRYCG	2 cut(s)	8, 12
BsiHKAI	GWGCWC	4 cut(s)	50, 465, 519, 763
BsiSI	CCGG	4 cut(s)	282, 393, 658, 1283
BslFI	GGGAC	1 cut(s)	418
BslI	CCNNNNNNNGG	7 cut(s)	346, 605, 775, 983, 1244, 1283, 1573
BsmAI	GTCTC	2 cut(s)	496, 1602
BsmFI	GGGAC	1 cut(s)	418
BsmI	GAATGC	2 cut(s)	855, 1789
BsnI	GGCC	1 cut(s)	1718
Bsp1286I	GDGCHC	4 cut(s)	50, 465, 519, 763
Bsp143I	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
Bsp1720I	GCTNAGC	1 cut(s)	369
Bsp19I	CCATGG	1 cut(s)	150
BspACI	CCGC	6 cut(s)	4, 12, 119, 1101, 1252, 1649
BspANI	GGCC	1 cut(s)	1718
BspCNI	CTCAG	7 cut(s)	93, 373, 389, 456, 495, 497, 1387
BspDI	ATCGAT	1 cut(s)	311
BspFNI	CGCG	3 cut(s)	14, 190, 1753
BspHI	TCATGA	2 cut(s)	1057, 1506
BspLI	GGNNCC	4 cut(s)	684, 1071, 1132, 1830
BspMI	ACCTGC	1 cut(s)	1693
BspPI	GGATC	5 cut(s)	677, 690, 994, 1064, 1077
BspQI	GCTCTTC	1 cut(s)	507
BspTI	CTTAAG	1 cut(s)	1240
BsrBI	CCGCTC	1 cut(s)	1252
BsrDI	GCAATG	3 cut(s)	131, 198, 1071
BsrFI	RCCGGY	2 cut(s)	281, 392
BsrI	ACTGG	5 cut(s)	578, 585, 1024, 1444, 1494
BssAI	RCCGGY	2 cut(s)	281, 392
BssECI	CCNNGG	5 cut(s)	150, 340, 774, 1643, 1778
BssMI	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
BssNAI	GTATAC	2 cut(s)	398, 795
BssT1I	CCWWGG	3 cut(s)	150, 340, 774
Bst1107I	GTATAC	2 cut(s)	398, 795
Bst2UI	CCWGG	1 cut(s)	1644
Bst4CI	ACNGT	5 cut(s)	230, 1012, 1110, 1267, 1456
Bst6I	CTCTTC	2 cut(s)	507, 1152
BstAFI	CTTAAG	1 cut(s)	1240
BstAPI	GCANNNNNTGC	1 cut(s)	54
BstC8I	GCNNGC	2 cut(s)	863, 1261
BstDEI	CTNAG	9 cut(s)	80, 369, 376, 381, 413, 443, 484, 503, 1395
BstDSI	CCRYGG	1 cut(s)	150
BstF5I	GGATG	1 cut(s)	146
BstFNI	CGCG	3 cut(s)	14, 190, 1753
BstHHI	GCGC	1 cut(s)	16
BstKTI	GATC	6 cut(s)	212, 217, 685, 808, 989, 1072
BstMAI	GTCTC	2 cut(s)	496, 1602
BstMBI	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
BstMCI	CGRYCG	2 cut(s)	8, 12
BstMWI	GCNNNNNNNGC	7 cut(s)	54, 118, 551, 961, 1061, 1091, 1100
BstNI	CCWGG	1 cut(s)	1644
BstNSI	RCATGY	2 cut(s)	473, 523
BstSCI	CCNGG	2 cut(s)	1282, 1642
BstSFI	CTRYAG	1 cut(s)	1332
BstSLI	GKGCMC	1 cut(s)	763
BstUI	CGCG	3 cut(s)	14, 190, 1753
BstV1I	GCAGC	3 cut(s)	529, 541, 900
BstV2I	GAAGAC	1 cut(s)	901
BstX2I	RGATCY	3 cut(s)	682, 986, 1069
BstYI	RGATCY	3 cut(s)	682, 986, 1069
BstZ17I	GTATAC	2 cut(s)	398, 795
Bsu15I	ATCGAT	1 cut(s)	311
Bsu36I	CCTNAGG	1 cut(s)	1395
BsuI	GTATCC	2 cut(s)	1421, 1514
BsuRI	GGCC	1 cut(s)	1718
BsuTUI	ATCGAT	1 cut(s)	311
BtgI	CCRYGG	1 cut(s)	150
BtsCI	GGATG	1 cut(s)	146
BtsIMutI	CAGTG	3 cut(s)	919, 1461, 1501
BveI	ACCTGC	1 cut(s)	1693
Cac8I	GCNNGC	2 cut(s)	863, 1261
CciI	TCATGA	2 cut(s)	1057, 1506
CfoI	GCGC	1 cut(s)	16
Cfr10I	RCCGGY	2 cut(s)	281, 392
Cfr13I	GGNCC	1 cut(s)	1716
ClaI	ATCGAT	1 cut(s)	311
CseI	GACGC	2 cut(s)	93, 1103
Csp6I	GTAC	4 cut(s)	507, 1452, 1471, 1491
CspAI	ACCGGT	1 cut(s)	281
CviQI	GTAC	4 cut(s)	507, 1452, 1471, 1491
DdeI	CTNAG	9 cut(s)	80, 369, 376, 381, 413, 443, 484, 503, 1395
DpnI	GATC	6 cut(s)	211, 216, 684, 807, 988, 1071
DpnII	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
DrdI	GACNNNNNNGTC	1 cut(s)	488
DseDI	GACNNNNNNGTC	1 cut(s)	488
Eam1104I	CTCTTC	2 cut(s)	507, 1152
EarI	CTCTTC	2 cut(s)	507, 1152
EciI	GGCGGA	1 cut(s)	1664
Ecl136II	GAGCTC	1 cut(s)	463
Eco130I	CCWWGG	3 cut(s)	150, 340, 774
Eco24I	GRGCYC	1 cut(s)	465
Eco32I	GATATC	2 cut(s)	264, 1504
Eco53kI	GAGCTC	1 cut(s)	463
Eco57I	CTGAAG	6 cut(s)	288, 474, 531, 895, 1329, 1417
Eco81I	CCTNAGG	1 cut(s)	1395
EcoICRI	GAGCTC	1 cut(s)	463
EcoRII	CCWGG	1 cut(s)	1642
EcoRV	GATATC	2 cut(s)	264, 1504
EcoT14I	CCWWGG	3 cut(s)	150, 340, 774
EcoT38I	GRGCYC	1 cut(s)	465
ErhI	CCWWGG	3 cut(s)	150, 340, 774
FalI	AAGNNNNNCTT	4 cut(s)	324, 356, 1713, 1745
FaqI	GGGAC	1 cut(s)	418
FbaI	TGATCA	2 cut(s)	214, 805
FblI	GTMKAC	4 cut(s)	8, 397, 794, 823
Fnu4HI	GCNGC	4 cut(s)	543, 555, 889, 1101
FokI	GGATG	1 cut(s)	133
FriOI	GRGCYC	1 cut(s)	465
Fsp4HI	GCNGC	4 cut(s)	543, 555, 889, 1101
FspBI	CTAG	2 cut(s)	351, 705
GlaI	GCGC	1 cut(s)	15
GluI	GCNGC	4 cut(s)	543, 555, 889, 1101
GsaI	CCCAGC	1 cut(s)	1571
GsuI	CTGGAG	2 cut(s)	1041, 1461
HaeIII	GGCC	1 cut(s)	1718
HapII	CCGG	4 cut(s)	282, 393, 658, 1283
HgaI	GACGC	2 cut(s)	93, 1103
HhaI	GCGC	1 cut(s)	16
Hin6I	GCGC	1 cut(s)	14
HinP1I	GCGC	1 cut(s)	14
HincII	GTYRAC	1 cut(s)	9
HindII	GTYRAC	1 cut(s)	9
HindIII	AAGCTT	3 cut(s)	783, 1388, 1693
HinfI	GANTC	5 cut(s)	158, 482, 1547, 1759, 1769
HpaII	CCGG	4 cut(s)	282, 393, 658, 1283
HphI	GGTGA	6 cut(s)	296, 588, 647, 1259, 1355, 1810
Hpy188I	TCNGA	5 cut(s)	83, 214, 487, 1436, 1782
Hpy188III	TCNNGA	7 cut(s)	267, 452, 1058, 1073, 1394, 1507, 1832
Hpy99I	CGWCG	1 cut(s)	112
HpyAV	CCTTC	5 cut(s)	84, 721, 851, 1504, 1663
HpyCH4III	ACNGT	5 cut(s)	230, 1012, 1110, 1267, 1456
HpyCH4IV	ACGT	1 cut(s)	1489
HpyF10VI	GCNNNNNNNGC	7 cut(s)	54, 118, 551, 961, 1061, 1091, 1100
HpyF3I	CTNAG	9 cut(s)	80, 369, 376, 381, 413, 443, 484, 503, 1395
HpySE526I	ACGT	1 cut(s)	1489
HspAI	GCGC	1 cut(s)	14
Ksp22I	TGATCA	2 cut(s)	214, 805
Kzo9I	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
LguI	GCTCTTC	1 cut(s)	507
LmnI	GCTCC	4 cut(s)	62, 460, 608, 1834
Lsp1109I	GCAGC	3 cut(s)	529, 541, 900
LweI	GCATC	5 cut(s)	263, 317, 628, 634, 1435
MaeI	CTAG	2 cut(s)	351, 705
MaeII	ACGT	1 cut(s)	1489
MaeIII	GTNAC	3 cut(s)	224, 230, 1399
MalI	GATC	6 cut(s)	211, 216, 684, 807, 988, 1071
MbiI	CCGCTC	1 cut(s)	1252
MboI	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
MboII	GAAGA	9 cut(s)	294, 524, 585, 901, 1139, 1322, 1475, 1685, 1828
MflI	RGATCY	3 cut(s)	682, 986, 1069
MhlI	GDGCHC	4 cut(s)	50, 465, 519, 763
MluI	ACGCGT	1 cut(s)	188
MlyI	GAGTC	2 cut(s)	167, 476
MmeI	TCCRAC	2 cut(s)	1052, 1249
MroXI	GAANNNNTTC	1 cut(s)	1551
MseI	TTAA	5 cut(s)	21, 171, 981, 1241, 1374
MspA1I	CMGCKG	2 cut(s)	380, 1571
MspCI	CTTAAG	1 cut(s)	1240
MspI	CCGG	4 cut(s)	282, 393, 658, 1283
MspR9I	CCNGG	2 cut(s)	1284, 1644
Mva1269I	GAATGC	2 cut(s)	855, 1789
MvaI	CCWGG	1 cut(s)	1644
MvnI	CGCG	3 cut(s)	14, 190, 1753
MwoI	GCNNNNNNNGC	7 cut(s)	54, 118, 551, 961, 1061, 1091, 1100
NciI	CCSGG	1 cut(s)	1284
NcoI	CCATGG	1 cut(s)	150
NdeII	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
NlaIV	GGNNCC	4 cut(s)	684, 1071, 1132, 1830
NspI	RCATGY	2 cut(s)	473, 523
PagI	TCATGA	2 cut(s)	1057, 1506
PciSI	GCTCTTC	1 cut(s)	507
PcsI	WCGNNNNNNNCGW	1 cut(s)	722
PctI	GAATGC	2 cut(s)	855, 1789
PdmI	GAANNNNTTC	1 cut(s)	1551
PfeI	GAWTC	3 cut(s)	1547, 1759, 1769
PflFI	GACNNNGTC	1 cut(s)	1185
PflMI	CCANNNNNTGG	3 cut(s)	605, 775, 983
PinAI	ACCGGT	1 cut(s)	281
PkrI	GCNGC	4 cut(s)	544, 556, 890, 1102
PleI	GAGTC	2 cut(s)	166, 476
PpsI	GAGTC	2 cut(s)	166, 476
PshBI	ATTAAT	1 cut(s)	981
Psp124BI	GAGCTC	1 cut(s)	465
Psp6I	CCWGG	1 cut(s)	1642
PspFI	CCCAGC	1 cut(s)	1567
PspGI	CCWGG	1 cut(s)	1642
PspN4I	GGNNCC	4 cut(s)	684, 1071, 1132, 1830
PspPI	GGNCC	1 cut(s)	1716
PsuI	RGATCY	3 cut(s)	682, 986, 1069
PsyI	GACNNNGTC	1 cut(s)	1185
PvuII	CAGCTG	2 cut(s)	380, 1571
RsaI	GTAC	4 cut(s)	508, 1453, 1472, 1492
RsaNI	GTAC	4 cut(s)	507, 1452, 1471, 1491
SacI	GAGCTC	1 cut(s)	465
SalI	GTCGAC	1 cut(s)	7
SapI	GCTCTTC	1 cut(s)	507
SaqAI	TTAA	5 cut(s)	21, 171, 981, 1241, 1374
SatI	GCNGC	4 cut(s)	543, 555, 889, 1101
Sau3AI	GATC	6 cut(s)	209, 214, 682, 805, 986, 1069
Sau96I	GGNCC	1 cut(s)	1716
ScaI	AGTACT	1 cut(s)	508
SchI	GAGTC	2 cut(s)	167, 476
ScrFI	CCNGG	2 cut(s)	1284, 1644
SduI	GDGCHC	4 cut(s)	50, 465, 519, 763
SfaNI	GCATC	5 cut(s)	263, 317, 628, 634, 1435
SfcI	CTRYAG	1 cut(s)	1332
SmlI	CTYRAG	1 cut(s)	1240
SmoI	CTYRAG	1 cut(s)	1240
SpeI	ACTAGT	1 cut(s)	704
SsiI	CCGC	6 cut(s)	4, 12, 119, 1101, 1252, 1649
SspI	AATATT	1 cut(s)	1382
SspMI	CTAG	2 cut(s)	351, 705
SstI	GAGCTC	1 cut(s)	465
StyD4I	CCNGG	2 cut(s)	1282, 1642
StyI	CCWWGG	3 cut(s)	150, 340, 774
TaaI	ACNGT	5 cut(s)	230, 1012, 1110, 1267, 1456
TaiI	ACGT	1 cut(s)	1492
TaqI	TCGA	5 cut(s)	8, 208, 311, 1042, 1841
TatI	WGTACW	1 cut(s)	506
TauI	GCSGC	1 cut(s)	1103
TfiI	GAWTC	3 cut(s)	1547, 1759, 1769
Tru1I	TTAA	5 cut(s)	21, 171, 981, 1241, 1374
Tru9I	TTAA	5 cut(s)	21, 171, 981, 1241, 1374
TscAI	CASTG	3 cut(s)	919, 1461, 1501
TseI	GCWGC	3 cut(s)	542, 554, 888
TspDTI	ATGAA	4 cut(s)	135, 1074, 1329, 1523
TspGWI	ACGGA	1 cut(s)	1353
TspRI	CASTG	3 cut(s)	919, 1461, 1501
Tth111I	GACNNNGTC	1 cut(s)	1185
Van91I	CCANNNNNTGG	3 cut(s)	605, 775, 983
Vha464I	CTTAAG	1 cut(s)	1240
VneI	GTGCAC	1 cut(s)	759
VspI	ATTAAT	1 cut(s)	981
XapI	RAATTY	1 cut(s)	932
XceI	RCATGY	2 cut(s)	473, 523
XmiI	GTMKAC	4 cut(s)	8, 397, 794, 823
XmnI	GAANNNNTTC	1 cut(s)	1551
XspI	CTAG	2 cut(s)	351, 705
ZrmI	AGTACT	1 cut(s)	508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.