RchiOBHm_Chr2g0139171

Malonate--CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006867
Physical Location & Seq
Forward (+)
56820533 .. 56823676
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50975

Sequence Viewer

Length: 984 bp
ATGAGCTCCACCACCCCTCCTTTATTTAAAGCTTTCAACTACTACTGCAGGTCACTCTTCTCCTCTTCTTGTTCATCTTGTTCGCGTTTCAAGGCCTCCCGCTATCTCACCAGGATCAACCCTTTGCTCTGCTTGTCACACTCTCGCCTTTTCTCTTCCTTGCAATCTGGTTCATCTATGGAGGCGGTAAAAGTAGCTTGTCAAGGTTCTGGAACTCAGGATAGTGTTGCTATTAGATCAGATAACAAGAGTTACAGTTACAATCAACTAATTTCTTCTGCATGGAGTATATCTGGCTTGTTATGTTGTGGCGACTTAAAAACTTCCAAAGCAAGTAGCCATCAATCTCCTTTAGCAACAACTGCTGGTGCACGAGGACTTGGGCATCTTGGAGGGGCTAGAATAGGTATTGTGGCTAAGCCCTCCGCGGAATTCGTTGCTGGTATACTTGGGACTTGGCTTAGTGGAGGTGTTGCAGTTCCACTTGCTCTGAGCTACCCTGAGGCTGAGCTCTTGCATGTGATGAATGATTCAGACATCTCCATGATATTGAGTACTGAAGATCATCAACAACTTATGGAAAACCTTGCTGCTAAATGTGCTGCTCAGTTTTCTCTTATTCCACCAGTTCCCAGTAGTACTACTTCACATGCAAGTGCAGGTGGTCATTTACAAGCGGGAGAAGTAGATGCAGATAGAATTTTTCACAGGAATGGGAAGCAATCAAGTGAGGATCCAGCATTAATAATATACACTAGTGGTACAACGGGTAAACCTAAAGGAGTTGTTCACACTCACAAAAGCATCAGTGCGCAGGTCCAAACCTTGGCAGAAGCTTGGGAGTATTCATCTGCTGATCAGTTTTTGCATTGTCTACCACTACATCATATCCATTCAATTTTGTATACTTGTGTGCAAATACTGTTTTTGAATACAATTGGAGCTTCCATTTTGCTTTCTGCTTCAATGTCTCCTTTCCTCTGA

Protein Analysis

327

Amino Acids

34.76

Weight (kDa)

8.04

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 126 - 307 2.3e-37 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 650
Acc16I TGCGCA 1 cut(s) 813
Acc36I ACCTGC 3 cut(s) 39, 650, 805
AccB7I CCANNNNNTGG 1 cut(s) 826
AccI GTMKAC 3 cut(s) 445, 874, 905
AccII CGCG 2 cut(s) 85, 428
AciI CCGC 5 cut(s) 100, 185, 426, 428, 677
AclWI GGATC 3 cut(s) 122, 728, 741
AcsI RAATTY 2 cut(s) 431, 699
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 3 cut(s) 556, 640, 763
AfiI CCNNNNNNNGG 1 cut(s) 826
AgsI TTSAA 5 cut(s) 37, 91, 897, 931, 966
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 7 cut(s) 6, 32, 197, 495, 511, 836, 944
AluI AGCT 7 cut(s) 6, 32, 197, 495, 511, 836, 944
Alw21I GWGCWC 3 cut(s) 8, 373, 513
Alw26I GTCTC 1 cut(s) 975
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 3 cut(s) 122, 728, 741
AoxI GGCC 1 cut(s) 93
ApaLI GTGCAC 1 cut(s) 369
ApeKI GCWGC 2 cut(s) 590, 602
ApoI RAATTY 2 cut(s) 431, 699
AseI ATTAAT 1 cut(s) 743
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 1 cut(s) 100
AvaII GGWCC 1 cut(s) 817
AxyI CCTNAGG 1 cut(s) 501
BaeGI GKGCMC 1 cut(s) 373
BamHI GGATCC 1 cut(s) 733
BanII GRGCYC 2 cut(s) 8, 513
BarI GAAGNNNNNNTAC 2 cut(s) 628, 660
BauI CACGAG 1 cut(s) 372
Bbv12I GWGCWC 3 cut(s) 8, 373, 513
BbvI GCAGC 2 cut(s) 577, 589
BccI CCATC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 112
BclI TGATCA 1 cut(s) 856
BcoDI GTCTC 1 cut(s) 975
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 2 cut(s) 399, 756
BfmI CTRYAG 1 cut(s) 46
BfuAI ACCTGC 3 cut(s) 39, 650, 805
BisI GCNGC 2 cut(s) 591, 603
BlpI GCTNAGC 2 cut(s) 417, 507
BlsI GCNGC 2 cut(s) 592, 604
BmcAI AGTACT 2 cut(s) 556, 640
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 817
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 3 cut(s) 394, 679, 813
BmuI ACTGGG 1 cut(s) 627
Bpu1102I GCTNAGC 2 cut(s) 417, 507
BsaJI CCNNGG 2 cut(s) 426, 825
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 826
Bse1I ACTGG 2 cut(s) 626, 633
Bse21I CCTNAGG 1 cut(s) 501
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 426, 825
BseLI CCNNNNNNNGG 1 cut(s) 826
BseMII CTCAG 5 cut(s) 230, 482, 492, 498, 620
BseNI ACTGG 2 cut(s) 626, 633
BseRI GAGGAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 2 cut(s) 577, 589
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 2 cut(s) 85, 428
BshFI GGCC 1 cut(s) 95
BsiHKAI GWGCWC 3 cut(s) 8, 373, 513
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 1 cut(s) 826
BsmAI GTCTC 1 cut(s) 975
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 8, 373, 513
Bsp143I GATC 5 cut(s) 114, 236, 562, 733, 856
Bsp1720I GCTNAGC 2 cut(s) 417, 507
BspACI CCGC 5 cut(s) 100, 185, 426, 428, 677
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 5 cut(s) 229, 483, 493, 499, 619
BspFNI CGCG 2 cut(s) 85, 428
BspLI GGNNCC 1 cut(s) 735
BspMAI CTGCAG 1 cut(s) 50
BspMI ACCTGC 3 cut(s) 39, 650, 805
BspPI GGATC 3 cut(s) 122, 728, 741
BsrI ACTGG 2 cut(s) 626, 633
BssECI CCNNGG 2 cut(s) 426, 825
BssMI GATC 5 cut(s) 114, 236, 562, 733, 856
BssNAI GTATAC 2 cut(s) 446, 906
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 825
Bst1107I GTATAC 2 cut(s) 446, 906
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 257, 924
Bst6I CTCTTC 3 cut(s) 62, 70, 160
BstAPI GCANNNNNTGC 1 cut(s) 362
BstDEI CTNAG 7 cut(s) 216, 417, 461, 491, 501, 507, 606
BstDSI CCRYGG 1 cut(s) 426
BstFNI CGCG 2 cut(s) 85, 428
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 5 cut(s) 117, 239, 565, 736, 859
BstMAI GTCTC 1 cut(s) 975
BstMBI GATC 5 cut(s) 114, 236, 562, 733, 856
BstMWI GCNNNNNNNGC 2 cut(s) 362, 599
BstNI CCWGG 1 cut(s) 112
BstNSI RCATGY 2 cut(s) 521, 653
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 2 cut(s) 85, 428
BstV1I GCAGC 2 cut(s) 577, 589
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
BstZ17I GTATAC 2 cut(s) 446, 906
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 814
BveI ACCTGC 3 cut(s) 39, 650, 805
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 1 cut(s) 817
Cfr42I CCGCGG 1 cut(s) 429
Csp6I GTAC 3 cut(s) 555, 639, 762
CviAII CATG 4 cut(s) 282, 518, 544, 650
CviQI GTAC 3 cut(s) 555, 639, 762
DdeI CTNAG 7 cut(s) 216, 417, 461, 491, 501, 507, 606
DpnI GATC 5 cut(s) 116, 238, 564, 735, 858
DpnII GATC 5 cut(s) 114, 236, 562, 733, 856
DraI TTTAAA 1 cut(s) 28
Eam1104I CTCTTC 3 cut(s) 62, 70, 160
EarI CTCTTC 3 cut(s) 62, 70, 160
Ecl136II GAGCTC 2 cut(s) 6, 511
Eco130I CCWWGG 1 cut(s) 825
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 2 cut(s) 8, 513
Eco47I GGWCC 1 cut(s) 817
Eco53kI GAGCTC 2 cut(s) 6, 511
Eco57I CTGAAG 1 cut(s) 579
Eco81I CCTNAGG 1 cut(s) 501
EcoICRI GAGCTC 2 cut(s) 6, 511
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 2 cut(s) 8, 513
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 4 cut(s) 285, 521, 547, 653
FaqI GGGAC 1 cut(s) 466
FatI CATG 4 cut(s) 281, 517, 543, 649
FauI CCCGC 2 cut(s) 107, 670
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 3 cut(s) 445, 874, 905
Fnu4HI GCNGC 2 cut(s) 591, 603
FriOI GRGCYC 2 cut(s) 8, 513
Fsp4HI GCNGC 2 cut(s) 591, 603
FspBI CTAG 2 cut(s) 399, 756
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 591, 603
HaeIII GGCC 1 cut(s) 95
HhaI GCGC 1 cut(s) 814
Hin1II CATG 4 cut(s) 285, 521, 547, 653
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HindIII AAGCTT 2 cut(s) 30, 834
HinfI GANTC 1 cut(s) 530
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 6 cut(s) 371, 446, 773, 790, 875, 906
Hpy188I TCNGA 4 cut(s) 241, 492, 535, 983
Hpy188III TCNNGA 2 cut(s) 210, 218
Hpy8I GTNNAC 6 cut(s) 371, 446, 773, 790, 875, 906
HpyCH4III ACNGT 2 cut(s) 257, 924
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 599
HpyF3I CTNAG 7 cut(s) 216, 417, 461, 491, 501, 507, 606
Hsp92II CATG 4 cut(s) 285, 521, 547, 653
HspAI GCGC 1 cut(s) 812
Ksp22I TGATCA 1 cut(s) 856
KspI CCGCGG 1 cut(s) 429
Kzo9I GATC 5 cut(s) 114, 236, 562, 733, 856
LmnI GCTCC 2 cut(s) 11, 941
Lsp1109I GCAGC 2 cut(s) 577, 589
LweI GCATC 3 cut(s) 394, 679, 813
MaeI CTAG 2 cut(s) 399, 756
MaeIII GTNAC 4 cut(s) 51, 135, 251, 257
MalI GATC 5 cut(s) 116, 238, 564, 735, 858
MboI GATC 5 cut(s) 114, 236, 562, 733, 856
MboII GAAGA 5 cut(s) 49, 57, 147, 267, 572
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 3 cut(s) 8, 373, 513
MluCI AATT 5 cut(s) 270, 431, 699, 897, 936
MseI TTAA 3 cut(s) 27, 317, 743
MslI CAYNNNNRTG 3 cut(s) 542, 654, 711
MspA1I CMGCKG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 936
MvaI CCWGG 1 cut(s) 112
MvnI CGCG 2 cut(s) 85, 428
MwoI GCNNNNNNNGC 2 cut(s) 362, 599
NdeII GATC 5 cut(s) 114, 236, 562, 733, 856
NlaIII CATG 4 cut(s) 285, 521, 547, 653
NlaIV GGNNCC 1 cut(s) 735
NmuCI GTSAC 2 cut(s) 51, 135
NsbI TGCGCA 1 cut(s) 813
NspI RCATGY 2 cut(s) 521, 653
PaqCI CACCTGC 1 cut(s) 650
PceI AGGCCT 1 cut(s) 95
PfeI GAWTC 1 cut(s) 530
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 2 cut(s) 592, 604
PshBI ATTAAT 1 cut(s) 743
Psp124BI GAGCTC 2 cut(s) 8, 513
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 735
PspPI GGNCC 1 cut(s) 817
PstI CTGCAG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 733
RsaI GTAC 3 cut(s) 556, 640, 763
RsaNI GTAC 3 cut(s) 555, 639, 762
RseI CAYNNNNRTG 3 cut(s) 542, 654, 711
SacI GAGCTC 2 cut(s) 8, 513
SacII CCGCGG 1 cut(s) 429
SaqAI TTAA 3 cut(s) 27, 317, 743
SatI GCNGC 2 cut(s) 591, 603
Sau3AI GATC 5 cut(s) 114, 236, 562, 733, 856
Sau96I GGNCC 1 cut(s) 817
ScaI AGTACT 2 cut(s) 556, 640
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 3 cut(s) 8, 373, 513
SfaNI GCATC 3 cut(s) 394, 679, 813
SfcI CTRYAG 1 cut(s) 46
Sfr303I CCGCGG 1 cut(s) 429
SgrBI CCGCGG 1 cut(s) 429
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 3 cut(s) 542, 654, 711
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 5 cut(s) 270, 431, 699, 897, 936
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 5 cut(s) 100, 185, 426, 428, 677
SspMI CTAG 2 cut(s) 399, 756
SstI GAGCTC 2 cut(s) 8, 513
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 2 cut(s) 257, 924
TasI AATT 5 cut(s) 270, 431, 699, 897, 936
TatI WGTACW 2 cut(s) 554, 638
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 3 cut(s) 27, 317, 743
Tru9I TTAA 3 cut(s) 27, 317, 743
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 2 cut(s) 51, 135
TseI GCWGC 2 cut(s) 590, 602
Tsp45I GTSAC 2 cut(s) 51, 135
TspDTI ATGAA 4 cut(s) 63, 162, 539, 837
TspRI CASTG 1 cut(s) 814
Van91I CCANNNNNTGG 1 cut(s) 826
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 817
VspI ATTAAT 1 cut(s) 743
XapI RAATTY 2 cut(s) 431, 699
XceI RCATGY 2 cut(s) 521, 653
XmiI GTMKAC 3 cut(s) 445, 874, 905
XspI CTAG 2 cut(s) 399, 756
ZrmI AGTACT 2 cut(s) 556, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.