MD17G1209500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007601
Physical Location & Seq
Forward (+)
25450230 .. 25453736
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1209500.v1.1.491

Sequence Viewer

Length: 933 bp
ATGTGGCCAAATTTATGGTGGGGAAATGTAAAAACATGTACGGTAAATAAGCGAGTACAAGCCCCACGAAAAATAATTAAATGGTGGGGAATCGAAGCTCCCACGCACGAATCCCATTATATAAACTTTCCATCTCAGTCGTACGTTTATTGTTCCGTTTCCTGCCCTTGCCATTGCCATCGAAGAACCAGAAGAAGGAAAACTCAAAGAATCCAAAGGGCCATCCCTTCTGGGCTTCTTTGCTTCAAGAAGTGGGTGCCTTTGGATTTCGATTTCAGGAATGAGAGCAAGAGACTGATATCAAAGCAGAAACATTTTCTCACGGGAGGAGGGGCTCTCTGGTTTTTCTTTCCTGCAATCGGTTTTCCATCGGATTTTGTTGCTGATTGGTTTTACATTTTCCTGGGAAAATCCTTAGATCTGATACTGCTTTCTGGGTCTTCCTTGTATTGCATGCATGGGAGCAGTGGTTGTCTAGCATGCTATAGTAAGAACGCACCGAAAACTAAAGTGAATGAGCCACCGAAAAGGTTGTTAAACGAACATCGGACTGAAAAGAAACCAAGCATATCAGAGGATTTCTGGACCACCAGCACGTGGGATATGGACAACAGTGCAGTTCAGTCCCAGGGAAGCATCTCATCAATCAGCACAAACCAGACGCTTGATCCGCATGGTGGCTCTGGCAGCAATAGCACCCCTTCCGAATTTGTAAATCATGGTCTTCTTCTCTGGAACCAGACTAGGCACCGTTGGGTAGGGAATAAAAAGTCTGAGAGCCCATCAAAGAAAATTCGAGAACCCAAATTAAGTTGGAATGCGTCATATGAAAGTTTACTTGGGAGTTCCAAGCCTTTCCCTCAGCCTATTCCTCTCCCGGAAATGGTAGATTTTCTCGTTGATATTTGGGAGCAAGAAGGGTTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

311

Amino Acids

35.58

Weight (kDa)

9.36

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 200 - 310 1.8e-25 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 256, 747
AccB7I CCANNNNNTGG 1 cut(s) 597
AciI CCGC 1 cut(s) 671
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 3 cut(s) 10, 707, 792
AcvI CACGTG 1 cut(s) 597
AfaI GTAC 4 cut(s) 40, 57, 143, 926
AfiI CCNNNNNNNGG 5 cut(s) 195, 359, 597, 677, 883
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 2 cut(s) 402, 627
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AluBI AGCT 1 cut(s) 98
AluI AGCT 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 662
AoxI GGCC 2 cut(s) 5, 219
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 3 cut(s) 10, 707, 792
AspS9I GGNCC 2 cut(s) 219, 585
AsuC2I CCSGG 1 cut(s) 878
AvaII GGWCC 1 cut(s) 585
BalI TGGCCA 1 cut(s) 7
BanI GGYRCC 2 cut(s) 256, 747
BanII GRGCYC 2 cut(s) 337, 782
BbrPI CACGTG 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 432, 716
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 1 cut(s) 699
BccI CCATC 5 cut(s) 139, 186, 230, 376, 790
BciT130I CCWGG 2 cut(s) 404, 629
BcnI CCSGG 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 2 cut(s) 476, 744
BfmI CTRYAG 1 cut(s) 484
BglII AGATCT 1 cut(s) 418
BisI GCNGC 1 cut(s) 688
BlsI GCNGC 1 cut(s) 689
Bme1390I CCNGG 3 cut(s) 404, 629, 878
Bme18I GGWCC 1 cut(s) 585
BmgT120I GGNCC 2 cut(s) 219, 585
BmiI GGNNCC 3 cut(s) 258, 737, 749
BmrFI CCNGG 3 cut(s) 404, 629, 878
BmsI GCATC 1 cut(s) 645
BpiI GAAGAC 2 cut(s) 432, 716
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu10I CCTNAGC 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 878
BsaAI YACGTR 1 cut(s) 597
BsaJI CCNNGG 3 cut(s) 403, 627, 628
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 359, 597, 677, 883
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 2 cut(s) 404, 629
BseDI CCNNGG 3 cut(s) 403, 627, 628
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 5 cut(s) 195, 359, 597, 677, 883
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 3 cut(s) 149, 765, 875
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 1 cut(s) 699
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 2 cut(s) 7, 221
BshNI GGYRCC 2 cut(s) 256, 747
BsiSI CCGG 1 cut(s) 878
BsiWI CGTACG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 5 cut(s) 195, 359, 597, 677, 883
BsmAI GTCTC 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 2 cut(s) 7, 221
Bsp1286I GDGCHC 2 cut(s) 337, 782
Bsp143I GATC 2 cut(s) 418, 667
BspACI CCGC 1 cut(s) 671
BspANI GGCC 2 cut(s) 7, 221
BspCNI CTCAG 3 cut(s) 148, 766, 874
BspLI GGNNCC 3 cut(s) 258, 737, 749
BspPI GGATC 1 cut(s) 662
BspT107I GGYRCC 2 cut(s) 256, 747
BsrDI GCAATG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 403, 627, 628
BssMI GATC 2 cut(s) 418, 667
Bst2UI CCWGG 2 cut(s) 404, 629
Bst4CI ACNGT 3 cut(s) 43, 614, 752
BstBAI YACGTR 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 455, 481
BstDEI CTNAG 4 cut(s) 135, 415, 774, 861
BstF5I GGATG 1 cut(s) 222
BstKTI GATC 2 cut(s) 421, 670
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 2 cut(s) 418, 667
BstMWI GCNNNNNNNGC 3 cut(s) 670, 687, 693
BstNI CCWGG 2 cut(s) 404, 629
BstNSI RCATGY 3 cut(s) 39, 457, 483
BstSCI CCNGG 3 cut(s) 402, 627, 876
BstSFI CTRYAG 1 cut(s) 484
BstV1I GCAGC 1 cut(s) 699
BstV2I GAAGAC 2 cut(s) 432, 716
BstX2I RGATCY 1 cut(s) 418
BstXI CCANNNNNNTGG 1 cut(s) 15
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 2 cut(s) 7, 221
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 472, 619
Cac8I GCNNGC 2 cut(s) 455, 481
Cfr13I GGNCC 2 cut(s) 219, 585
CseI GACGC 2 cut(s) 670, 810
Csp6I GTAC 4 cut(s) 39, 56, 142, 925
CviAII CATG 6 cut(s) 36, 454, 458, 480, 674, 719
CviQI GTAC 4 cut(s) 39, 56, 142, 925
DdeI CTNAG 4 cut(s) 135, 415, 774, 861
DpnI GATC 2 cut(s) 420, 669
DpnII GATC 2 cut(s) 418, 667
EaeI YGGCCR 1 cut(s) 5
Eco24I GRGCYC 2 cut(s) 337, 782
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 585
Eco72I CACGTG 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 402, 627
EcoRV GATATC 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 337, 782
FaeI CATG 6 cut(s) 39, 457, 461, 483, 677, 722
FaqI GGGAC 1 cut(s) 610
FatI CATG 6 cut(s) 35, 453, 457, 479, 673, 718
FauNDI CATATG 1 cut(s) 826
Fnu4HI GCNGC 1 cut(s) 688
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 2 cut(s) 337, 782
Fsp4HI GCNGC 1 cut(s) 688
FspBI CTAG 2 cut(s) 476, 744
GluI GCNGC 1 cut(s) 688
HaeIII GGCC 2 cut(s) 7, 221
HapII CCGG 1 cut(s) 878
HgaI GACGC 2 cut(s) 670, 810
Hin1II CATG 6 cut(s) 39, 457, 461, 483, 677, 722
HinfI GANTC 3 cut(s) 90, 110, 210
HpaII CCGG 1 cut(s) 878
Hpy166II GTNNAC 1 cut(s) 836
Hpy188I TCNGA 6 cut(s) 373, 423, 549, 574, 706, 775
Hpy188III TCNNGA 5 cut(s) 247, 277, 583, 733, 797
Hpy8I GTNNAC 1 cut(s) 836
HpyAV CCTTC 4 cut(s) 189, 237, 711, 911
HpyCH4III ACNGT 3 cut(s) 43, 614, 752
HpyCH4IV ACGT 2 cut(s) 144, 596
HpyCH4V TGCA 4 cut(s) 356, 453, 457, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 670, 687, 693
HpyF3I CTNAG 4 cut(s) 135, 415, 774, 861
HpySE526I ACGT 2 cut(s) 144, 596
Hsp92II CATG 6 cut(s) 39, 457, 461, 483, 677, 722
Kzo9I GATC 2 cut(s) 418, 667
LmnI GCTCC 3 cut(s) 103, 462, 910
Lsp1109I GCAGC 1 cut(s) 699
LweI GCATC 1 cut(s) 645
MaeI CTAG 2 cut(s) 476, 744
MaeII ACGT 2 cut(s) 144, 596
MalI GATC 2 cut(s) 420, 669
MboI GATC 2 cut(s) 418, 667
MboII GAAGA 5 cut(s) 195, 204, 432, 716, 719
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 337, 782
MlsI TGGCCA 1 cut(s) 7
MluCI AATT 5 cut(s) 10, 75, 707, 792, 806
MluNI TGGCCA 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 794
MnlI CCTC 5 cut(s) 320, 323, 568, 870, 882
Mox20I TGGCCA 1 cut(s) 7
Mph1103I ATGCAT 1 cut(s) 459
MscI TGGCCA 1 cut(s) 7
MseI TTAA 3 cut(s) 78, 536, 809
Msp20I TGGCCA 1 cut(s) 7
MspI CCGG 1 cut(s) 878
MspR9I CCNGG 3 cut(s) 404, 629, 878
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 2 cut(s) 404, 629
MwoI GCNNNNNNNGC 3 cut(s) 670, 687, 693
NciI CCSGG 1 cut(s) 878
NdeI CATATG 1 cut(s) 826
NdeII GATC 2 cut(s) 418, 667
NlaIII CATG 6 cut(s) 39, 457, 461, 483, 677, 722
NlaIV GGNNCC 3 cut(s) 258, 737, 749
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 3 cut(s) 39, 457, 483
PaeI GCATGC 2 cut(s) 457, 483
PasI CCCWGGG 1 cut(s) 628
PciI ACATGT 1 cut(s) 35
PctI GAATGC 1 cut(s) 823
PfeI GAWTC 3 cut(s) 90, 110, 210
Pfl23II CGTACG 1 cut(s) 141
PflMI CCANNNNNTGG 1 cut(s) 597
PfoI TCCNGGA 1 cut(s) 876
PkrI GCNGC 1 cut(s) 689
PmaCI CACGTG 1 cut(s) 597
PmlI CACGTG 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 597
PscI ACATGT 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 402, 627
PspCI CACGTG 1 cut(s) 597
PspGI CCWGG 2 cut(s) 402, 627
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 3 cut(s) 258, 737, 749
PspPI GGNCC 2 cut(s) 219, 585
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 4 cut(s) 40, 57, 143, 926
RsaNI GTAC 4 cut(s) 39, 56, 142, 925
SaqAI TTAA 3 cut(s) 78, 536, 809
SatI GCNGC 1 cut(s) 688
Sau3AI GATC 2 cut(s) 418, 667
Sau96I GGNCC 2 cut(s) 219, 585
ScrFI CCNGG 3 cut(s) 404, 629, 878
SduI GDGCHC 2 cut(s) 337, 782
SetI ASST 4 cut(s) 100, 147, 533, 599
SfaNI GCATC 1 cut(s) 645
SfcI CTRYAG 1 cut(s) 484
SinI GGWCC 1 cut(s) 585
SphI GCATGC 2 cut(s) 457, 483
Sse9I AATT 5 cut(s) 10, 75, 707, 792, 806
SsiI CCGC 1 cut(s) 671
SspMI CTAG 2 cut(s) 476, 744
StyD4I CCNGG 3 cut(s) 402, 627, 876
TaaI ACNGT 3 cut(s) 43, 614, 752
TaiI ACGT 2 cut(s) 147, 599
TaqI TCGA 4 cut(s) 93, 181, 270, 796
TasI AATT 5 cut(s) 10, 75, 707, 792, 806
TatI WGTACW 1 cut(s) 55
TfiI GAWTC 3 cut(s) 90, 110, 210
Tru1I TTAA 3 cut(s) 78, 536, 809
Tru9I TTAA 3 cut(s) 78, 536, 809
TscAI CASTG 2 cut(s) 472, 619
TseI GCWGC 1 cut(s) 687
TspDTI ATGAA 1 cut(s) 843
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 472, 619
Van91I CCANNNNNTGG 1 cut(s) 597
VpaK11BI GGWCC 1 cut(s) 585
XapI RAATTY 3 cut(s) 10, 707, 792
XceI RCATGY 3 cut(s) 39, 457, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 15
XspI CTAG 2 cut(s) 476, 744
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.