Rorug02G0326500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007601
Physical Location & Seq
Forward (+)
40062657 .. 40065084
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0326500.1

Sequence Viewer

Length: 1311 bp
ATGGATTCGACAGCTTCACTGCCTCCCTATCGTCACCATTCTCGTTCGGAGCCACCGTCCCGAACCAAAACGGCGTCACGGCTCGCCCAATCGGAGAAGGTCCCTCATCTCTCTCTGGGTCTCATTCAATCACCTTCACCTCCGTCTCGAAAATCTTCAGCTTCTCTCCCTCTACACGAGCTGCTGCTACTCTCGCCTTCGCCTAATCCAAGAAGGTCCAAGACCCGTCTCGCCGATCGCTTTGAAATGCCCGATGAGCCTGCGGCGGAACCGGTAGGCCCGCGACGGAGGTGCAAGAGCAGCAGGGGCTCCCAGATGGGCTCTCTGGGCTGTGCCTCGCCCAGGAACACCAGAAGGTCCAGGAGGAGATCGGAGGTTGAGATTCGAGAAGATAAGGATTTGGGTTTGGGGGAAGAGATTGTCAAGGTCAGGAAAAGAAGGTCCAAGAAGGACAAGCTGAGCTTGGTCCCTTGTGTGCTGCCCTCCCCTACTTCATCTATAATGGATGATGACGATGGGAGTCGTCTGGAGCGAATTGGGCAGCTATTGAATGATCTGATCATGTGGAGAGATGTAGCAAGGTCGACCCTTTGGTTTGGTTTGGGCTCTCTGTTCTTCTTGTCTTCTTGCTTTGCCAAAGGAGTGAACTTTAGCGTTTTCTCGGCGATTTCCCAACTAGGACTTCTGTTCTTGGGTGCTTCATTTGTCTCGAATTCAATCTCCCAAAGAAATGGTGTCGAAAAGAAGCGGGATTACTTGCTTAAGGAAAATGATGTCCTTGGTGTGGCCAAAATGATACTCCCTGCAGCAAACCTTGCAATATCAAGTACAAGGAAGCTTTTCTCAGGAGAGCCATCTACGACTCTCAAGGTAGCTCCATTCTTTCTTCTAGGAGCAGAGTATGGACATCATATAACATTGTGGAGGCTCTCTGTGCTTGCATTCTTTACCAGCTTCACAATCCCAAGGCTGTACTCATGCTACTCCGTTCAAATGAACCAAAGAGCTGAGTGCTTGAAATGGTGGGTGTTGGAAGCATGGGGAGCATGCTCTCACAAAAAGATTGTAGCAGCATCAGCAGCCACAGCATTCTGGAATCTGTCTACGGTGAAAACCCGCATATTCACCGCATTCATATCCCTTGTAATTCTTCGTTACTGCCGACAACACATGGTGGAAGTTAGGGAAGCAGATGCAGAAGCAGAACAGGAGGAGCAGCAGCAGGCCATAGTAGTGGCAGAAGCAGAAGGAAAACAGGAGCAGCAGCAAGAGCAGGCATTAGTAGTGGCCGAAGTGGGCAAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

436

Amino Acids

48.63

Weight (kDa)

9.65

Isoelectric Point (pI)

65.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 184 - 337 1.8e-27 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 584, 1103
AccII CGCG 1 cut(s) 283
AciI CCGC 6 cut(s) 263, 266, 281, 748, 1117, 1128
AcoI YGGCCR 2 cut(s) 786, 1287
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 1174
AfaI GTAC 2 cut(s) 829, 974
AfiI CCNNNNNNNGG 2 cut(s) 342, 784
AflII CTTAAG 1 cut(s) 761
AgeI ACCGGT 1 cut(s) 271
AgsI TTSAA 6 cut(s) 128, 245, 550, 717, 992, 1018
AjnI CCWGG 2 cut(s) 341, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
Alw26I GTCTC 4 cut(s) 125, 150, 233, 712
AoxI GGCC 4 cut(s) 277, 786, 1224, 1287
ApoI RAATTY 1 cut(s) 712
AsiGI ACCGGT 1 cut(s) 271
Asp700I GAANNNNTTC 2 cut(s) 154, 839
AspS9I GGNCC 6 cut(s) 100, 216, 278, 357, 441, 466
AsuHPI GGTGA 5 cut(s) 26, 123, 129, 1117, 1120
AvaII GGWCC 5 cut(s) 100, 216, 357, 441, 466
BalI TGGCCA 1 cut(s) 788
BanII GRGCYC 3 cut(s) 311, 323, 608
BarI GAAGNNNNNNTAC 2 cut(s) 737, 769
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 615
BccI CCATC 3 cut(s) 310, 509, 862
BceAI ACGGC 2 cut(s) 87, 95
BcgI CGANNNNNNTGC 4 cut(s) 242, 273, 276, 307
BciT130I CCWGG 2 cut(s) 343, 361
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 4 cut(s) 125, 150, 233, 712
BfaI CTAG 2 cut(s) 677, 890
BfmI CTRYAG 1 cut(s) 804
BfrI CTTAAG 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 343, 361
Bme18I GGWCC 5 cut(s) 100, 216, 357, 441, 466
BmgT120I GGNCC 6 cut(s) 100, 216, 278, 357, 441, 466
BmiI GGNNCC 5 cut(s) 51, 102, 270, 310, 468
BmrFI CCNGG 2 cut(s) 343, 361
BmsI GCATC 2 cut(s) 1082, 1183
BpiI GAAGAC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 851
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 341, 778, 965
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 784
Bse118I RCCGGY 1 cut(s) 271
BseBI CCWGG 2 cut(s) 343, 361
BseDI CCNNGG 3 cut(s) 341, 778, 965
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 342, 784
BseMII CTCAG 3 cut(s) 449, 858, 999
BseRI GAGGAG 2 cut(s) 379, 1226
Bsh1236I CGCG 1 cut(s) 283
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 4 cut(s) 279, 788, 1226, 1289
BshTI ACCGGT 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 3 cut(s) 43, 86, 452
BslI CCNNNNNNNGG 2 cut(s) 342, 784
BsmAI GTCTC 4 cut(s) 125, 150, 233, 712
BsmBI CGTCTC 2 cut(s) 150, 233
BsmFI GGGAC 3 cut(s) 43, 86, 452
BsmI GAATGC 3 cut(s) 941, 1088, 1130
BsnI GGCC 4 cut(s) 279, 788, 1226, 1289
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 311, 323, 608
Bsp143I GATC 4 cut(s) 235, 368, 553, 558
Bsp1720I GCTNAGC 1 cut(s) 458
BspACI CCGC 6 cut(s) 263, 266, 281, 748, 1117, 1128
BspANI GGCC 4 cut(s) 279, 788, 1226, 1289
BspCNI CTCAG 3 cut(s) 450, 857, 1000
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 5 cut(s) 51, 102, 270, 310, 468
BspMAI CTGCAG 1 cut(s) 808
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 271
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 3 cut(s) 341, 778, 965
BssMI GATC 4 cut(s) 235, 368, 553, 558
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 778, 965
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 343, 361
Bst4CI ACNGT 2 cut(s) 57, 1108
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 8 cut(s) 84, 261, 281, 939, 1048, 1224, 1275, 1301
BstDEI CTNAG 3 cut(s) 458, 844, 1008
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 1 cut(s) 283
BstKTI GATC 4 cut(s) 238, 371, 556, 561
BstMAI GTCTC 4 cut(s) 125, 150, 233, 712
BstMBI GATC 4 cut(s) 235, 368, 553, 558
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 2 cut(s) 343, 361
BstNSI RCATGY 1 cut(s) 1050
BstSCI CCNGG 2 cut(s) 341, 359
BstSFI CTRYAG 1 cut(s) 804
BstUI CGCG 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 615
BstXI CCANNNNNNTGG 2 cut(s) 731, 1234
BsuRI GGCC 4 cut(s) 279, 788, 1226, 1289
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 8 cut(s) 84, 261, 281, 939, 1048, 1224, 1275, 1301
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 6 cut(s) 100, 216, 278, 357, 441, 466
CseI GACGC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 828, 973
CspAI ACCGGT 1 cut(s) 271
CviAII CATG 5 cut(s) 562, 978, 1038, 1047, 1171
CviQI GTAC 2 cut(s) 828, 973
DdeI CTNAG 3 cut(s) 458, 844, 1008
DpnI GATC 4 cut(s) 237, 370, 555, 560
DpnII GATC 4 cut(s) 235, 368, 553, 558
DraIII CACNNNGTG 1 cut(s) 1174
EaeI YGGCCR 2 cut(s) 786, 1287
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 778, 965
Eco24I GRGCYC 3 cut(s) 311, 323, 608
Eco31I GGTCTC 1 cut(s) 125
Eco47I GGWCC 5 cut(s) 100, 216, 357, 441, 466
Eco57I CTGAAG 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 2 cut(s) 341, 359
EcoT14I CCWWGG 2 cut(s) 778, 965
EcoT38I GRGCYC 3 cut(s) 311, 323, 608
ErhI CCWWGG 2 cut(s) 778, 965
Esp3I CGTCTC 2 cut(s) 150, 233
FaeI CATG 5 cut(s) 565, 981, 1041, 1050, 1174
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 3 cut(s) 43, 86, 452
FatI CATG 5 cut(s) 561, 977, 1037, 1046, 1170
FauI CCCGC 3 cut(s) 288, 741, 1124
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 2 cut(s) 584, 1103
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 3 cut(s) 311, 323, 608
FspBI CTAG 2 cut(s) 677, 890
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 4 cut(s) 279, 788, 1226, 1289
HapII CCGG 1 cut(s) 272
HgaI GACGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 5 cut(s) 565, 981, 1041, 1050, 1174
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 5 cut(s) 5, 382, 520, 862, 1096
HpaII CCGG 1 cut(s) 272
HphI GGTGA 5 cut(s) 26, 123, 129, 1117, 1120
Hpy166II GTNNAC 3 cut(s) 585, 646, 1104
Hpy188I TCNGA 4 cut(s) 49, 94, 373, 558
Hpy188III TCNNGA 8 cut(s) 60, 147, 386, 430, 527, 709, 846, 1093
Hpy8I GTNNAC 3 cut(s) 585, 646, 1104
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 8 cut(s) 91, 144, 207, 207, 348, 432, 442, 1241
HpyCH4III ACNGT 2 cut(s) 57, 1108
HpyCH4V TGCA 5 cut(s) 294, 806, 818, 941, 1196
HpyF3I CTNAG 3 cut(s) 458, 844, 1008
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 5 cut(s) 565, 981, 1041, 1050, 1174
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 4 cut(s) 235, 368, 553, 558
LmnI GCTCC 8 cut(s) 49, 314, 529, 880, 893, 1043, 1213, 1258
LweI GCATC 2 cut(s) 1082, 1183
MaeI CTAG 2 cut(s) 677, 890
MaeIII GTNAC 3 cut(s) 32, 75, 1154
MalI GATC 4 cut(s) 237, 370, 555, 560
MboI GATC 4 cut(s) 235, 368, 553, 558
MboII GAAGA 7 cut(s) 147, 401, 425, 607, 615, 878, 1142
MhlI GDGCHC 3 cut(s) 311, 323, 608
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 3 cut(s) 534, 712, 1146
MluNI TGGCCA 1 cut(s) 788
MlyI GAGTC 2 cut(s) 529, 856
MmeI TCCRAC 1 cut(s) 1011
Mox20I TGGCCA 1 cut(s) 788
MroXI GAANNNNTTC 2 cut(s) 154, 839
MscI TGGCCA 1 cut(s) 788
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 1 cut(s) 1232
Msp20I TGGCCA 1 cut(s) 788
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 343, 361
Mva1269I GAATGC 3 cut(s) 941, 1088, 1130
MvaI CCWGG 2 cut(s) 343, 361
MvnI CGCG 1 cut(s) 283
NdeII GATC 4 cut(s) 235, 368, 553, 558
NlaIII CATG 5 cut(s) 565, 981, 1041, 1050, 1174
NlaIV GGNNCC 5 cut(s) 51, 102, 270, 310, 468
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 2 cut(s) 32, 75
NspI RCATGY 1 cut(s) 1050
PaeI GCATGC 1 cut(s) 1050
PcsI WCGNNNNNNNCGW 3 cut(s) 53, 529, 1159
PctI GAATGC 3 cut(s) 941, 1088, 1130
PdmI GAANNNNTTC 2 cut(s) 154, 839
PfeI GAWTC 3 cut(s) 5, 382, 1096
PfoI TCCNGGA 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 271
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 2 cut(s) 528, 856
PpsI GAGTC 2 cut(s) 528, 856
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 341, 359
PspGI CCWGG 2 cut(s) 341, 359
PspN4I GGNNCC 5 cut(s) 51, 102, 270, 310, 468
PspPI GGNCC 6 cut(s) 100, 216, 278, 357, 441, 466
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 1 cut(s) 808
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 829, 974
RsaNI GTAC 2 cut(s) 828, 973
RseI CAYNNNNRTG 1 cut(s) 1232
SalI GTCGAC 1 cut(s) 583
SaqAI TTAA 1 cut(s) 762
Sau3AI GATC 4 cut(s) 235, 368, 553, 558
Sau96I GGNCC 6 cut(s) 100, 216, 278, 357, 441, 466
SchI GAGTC 2 cut(s) 529, 856
ScrFI CCNGG 2 cut(s) 343, 361
SduI GDGCHC 3 cut(s) 311, 323, 608
SfaNI GCATC 2 cut(s) 1082, 1183
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 5 cut(s) 100, 216, 357, 441, 466
SmiMI CAYNNNNRTG 1 cut(s) 1232
SmlI CTYRAG 2 cut(s) 761, 866
SmoI CTYRAG 2 cut(s) 761, 866
SphI GCATGC 1 cut(s) 1050
Sse9I AATT 3 cut(s) 534, 712, 1146
SsiI CCGC 6 cut(s) 263, 266, 281, 748, 1117, 1128
SspMI CTAG 2 cut(s) 677, 890
StyD4I CCNGG 2 cut(s) 341, 359
StyI CCWWGG 2 cut(s) 778, 965
TaaI ACNGT 2 cut(s) 57, 1108
TaqI TCGA 6 cut(s) 8, 148, 385, 584, 710, 738
TasI AATT 3 cut(s) 534, 712, 1146
TatI WGTACW 2 cut(s) 827, 972
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 3 cut(s) 5, 382, 1096
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 2 cut(s) 32, 75
Tsp45I GTSAC 2 cut(s) 32, 75
TspDTI ATGAA 4 cut(s) 483, 690, 1010, 1123
TspGWI ACGGA 3 cut(s) 132, 301, 976
TspRI CASTG 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 5 cut(s) 100, 216, 357, 441, 466
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 1 cut(s) 712
XceI RCATGY 1 cut(s) 1050
XmiI GTMKAC 2 cut(s) 584, 1103
XmnI GAANNNNTTC 2 cut(s) 154, 839
XspI CTAG 2 cut(s) 677, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.