MD17G1242300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006849
Physical Location & Seq
Forward (+)
29152193 .. 29156263
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1242300.v1.1.491

Sequence Viewer

Length: 2523 bp
ATGTACAGTAGAATTATATGTCCAGTCTACAAACGTTTCATACACATTACACCACAATGGTCCAAGTCCATACATACAAACTACAAAAACTCCCTTAATTTGGAAGGGGCATTGGCAAGCCAGTTTGCTTCTATCTTGCAAGCTTGCAATGATGAGTCTCTTATCCAACAAGGTAGACAAGTTCATGCACATGTTATTTGCAGCGGACTTGTCAAAAACAGCCTTGTCTGGACAAGGATCCTGGGTATGTATTTTCTCTGCGGGAGCATTATGGGCGCCAAGAACATGTTTTATCAGCTTGATTTGAAATATACTTTGCCTTGGAACTCGATGATTAGAGGGTTTACTATGATGGGTTACTTTGATTATGCCTTGCTGTTTTACTTTAAGATGCTGAGTTCTGGAGTTTCACCTGACAAGTACACTTTTTTGAGTGTAATTAAAGCTTGTGGTGGTGTGAATAATGTAAGATTGGGCAAAGCAGTATATGATACTATCCAGTTTATGGGTTTTGGAGTTGATATATTTGTGGGGAGCTCCTTGATTCGACTGTATGTGGACAACGGTTGTATTCAGGATGCATGGAGCTTGTTTGTTAAAATGCCCATCAAAGATTGTGTTTTATGGAATGTTATGCTTCATGGTTATGTTAAAAATGGACAATTAAATAATTCTGTTAGAATGTTCTTGGAAATGAGAAGTAGTGAAGTTAAGCCAAATGCAGTGACGTTTGCTTGCATTTTGTCTCTTTGTGCTTCAGAAGCTATGATTGGTTTTGGTACTCAACTTCATGGGCTTGTTGTTGCATGTGGGTTGGAGTCGGATTCTCCAGTGGCTAATACATTGTTAGCAATGTATTCTAAATGCCAATGCTTCTCTGATGCCCGCAAGTTGTTTGATATGATGCCTCGAACTGATCTAGTGACATGGAATGGAATGATATCTGGTTATATACAAAGTGGGTTTATGCTTGAGGCTTCACGTTTGTTTCAGGACATGATATCTACAAGTGTCAAACCTGATTCGATCACATTTGCAAGTTTTTTACCTTCGGTTGCTGAACTAGGTAGCCTCAAGCAAGGTAAGGAAATTCACGGTTACATTGTAAGGCACTGCGTGCCTTTGGATGTGTTTTTGCAATGTGCACTTATTGATGTATACTTCAAGTGCAGGAATGTGGACATGGCACGTAAAATTTTCAGCAAAAGCACCAGAACGGATGTTGTCATCTGCACAGCTATGATTTCAGGTTTTGTTCTCAATGGGATGAATTGTGATGCATTGGAAATTTTTAGAAGGTTACTTAAAGAGAAAATGAGACCGAATTCTCTAACACTGGCAAGTGTTCTACCAGCTTGTGCTGGTCTGGCTGCTCTGAAATTTGGAAAGGAATTGCATGGAAACATCCTTAAGCATGGGCTTGATGCAAGGTGTTATGTGGGTAGTGCCCTTACAGACATGTATGCAAAATCTGGAAGACTCGATCTTGCTCATCGAGTTTTTCAAAGACTGTCTGAAAGGGATGCTGTTTGTTGGAACTCGATGATAACAAGCTATTCCCAGAATGGCAAACCGGAAGAGGCGATAGATCTATTTCGTCAAATGGGGACGGAAGGTGCCAAGTATGACTGTGTTAGCATTTCAGCTGCGCTTTCTGCTTGTGCAAACTTACCAGCACTTCATTATGGGAAAGAGATCCATAGTTTCATGATTAGAAGTGCATTTAGTTCCGATCTATTTGCTGAGAGTGCACTGATAGACATGTATGCCAAATGTGGAAACTTAGATCTTGCTCGCCGTGTGTTTGACATGATGGAAGAGAAGAATGAAGTTTCCTGGAACAGCATCATTTCTGCTTATGGGAATCATGGTCGCCTTAAGGAATCTCTTGTTCTGTTTCATGAAATGTTGAGTAAAGGGATCTTGCCCGATCATGTCACCTTTCTTGGTATATTATCTGCTTGTGGCCATGCAGGCGCAGTTGATGATGGAATTCTCTACTTCCGTTGCATGATTGAGGAATATAGGATCCCAGCTAGGTTGGAGCATTATGCTTGCATGGTAGATCTATTAGGGCGTGCTGGACATTTACGTGAAGCATTTGAAACAATAAAGAGCATGCCATTCTCCCCAGATTCGGGTGTCTGGGGAACAATGCTTGGAGCCTGTCGGCTCCATGGCAATGTTGAGCTTGCAGAAGAGGTATCAAGATACCTTTTTGAATTGGCACCGCAAAATTCTGGCTATTATATACTCCTCTCAAATATACTTGCTGATGCTGGAAAATGGGGGAGTGTGCTGAAGGTTCGAACGCTAATGAAAAACAGAGAAGTTCAAAAGGTTCCTGGGTACAGTTGGATTGAGGTAAACAACAACACTCATATGTTTGTTGCAGCAGATGGGAGTCACCCACAGTCTGCTCAGATATATTCATTGCTCAACAGTCTTCTTCTAGAACTAAGAAAAGAGGGTTATAATCCTAGACCTTACCTTCCAACACATCCACAGACATTGGGGATTTAA

Protein Analysis

841

Amino Acids

93.82

Weight (kDa)

8.39

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 150 5.6e-07 PPR repeat family
PPR_2 PF13041 205 - 252 8.4e-11 PPR repeat family
PPR_2 PF13041 306 - 348 4.8e-09 PPR repeat family
PPR PF01535 308 - 335 2.2e-06 PPR repeat
PPR_2 PF13041 508 - 556 1.2e-09 PPR repeat family
PPR PF01535 510 - 539 7.4e-09 PPR repeat
PPR_2 PF13041 609 - 656 9.5e-12 PPR repeat family
PPR PF01535 611 - 641 2.2e-08 PPR repeat
E_motif PF20431 726 - 788 1e-18 E motif
Eplus_motif PF20430 793 - 818 5.8e-06 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2475
AccB1I GGYRCC 3 cut(s) 275, 1616, 2227
AccB7I CCANNNNNTGG 1 cut(s) 505
AccI GTMKAC 3 cut(s) 27, 175, 1158
AciI CCGC 4 cut(s) 204, 261, 886, 2231
AclI AACGTT 1 cut(s) 34
AclWI GGATC 6 cut(s) 232, 245, 1690, 1928, 2023, 2036
AcoI YGGCCR 1 cut(s) 1966
AcsI RAATTY 7 cut(s) 1089, 1194, 1287, 1324, 1379, 1992, 2236
AcuI CTGAAG 2 cut(s) 741, 2321
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 1117
AfaI GTAC 4 cut(s) 5, 422, 781, 2351
AfiI CCNNNNNNNGG 4 cut(s) 100, 505, 2137, 2138
AflII CTTAAG 2 cut(s) 1409, 1877
AflIII ACRYGT 4 cut(s) 190, 285, 1458, 1761
AgsI TTSAA 6 cut(s) 307, 1165, 1505, 2105, 2222, 2336
AjnI CCWGG 3 cut(s) 240, 1835, 2344
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
Alw21I GWGCWC 3 cut(s) 539, 1147, 1753
Alw26I GTCTC 3 cut(s) 162, 750, 1312
Alw44I GTGCAC 2 cut(s) 1143, 1749
AlwI GGATC 6 cut(s) 232, 245, 1690, 1928, 2023, 2036
AoxI GGCC 1 cut(s) 1966
ApaLI GTGCAC 2 cut(s) 1143, 1749
ApeKI GCWGC 4 cut(s) 201, 1370, 1646, 2393
ApoI RAATTY 7 cut(s) 1089, 1194, 1287, 1324, 1379, 1992, 2236
AspLEI GCGC 3 cut(s) 278, 1651, 1979
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 402, 1930, 2399
AsuII TTCGAA 1 cut(s) 2308
AvaII GGWCC 1 cut(s) 60
BaeGI GKGCMC 3 cut(s) 1147, 1450, 1753
BalI TGGCCA 1 cut(s) 1968
BamHI GGATCC 2 cut(s) 237, 2028
BanI GGYRCC 3 cut(s) 275, 1616, 2227
BanII GRGCYC 1 cut(s) 539
BbsI GAAGAC 2 cut(s) 1483, 2438
Bbv12I GWGCWC 3 cut(s) 539, 1147, 1753
BbvI GCAGC 4 cut(s) 213, 1357, 1633, 2405
BccI CCATC 5 cut(s) 346, 614, 1807, 1982, 2393
BceAI ACGGC 1 cut(s) 1782
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 3 cut(s) 242, 1837, 2346
BcoDI GTCTC 3 cut(s) 162, 750, 1312
BfaI CTAG 5 cut(s) 920, 1064, 2037, 2453, 2481
BfoI RGCGCY 1 cut(s) 279
BfrI CTTAAG 2 cut(s) 1409, 1877
BglI GCCNNNNNGGC 1 cut(s) 1974
BglII AGATCT 3 cut(s) 1588, 1786, 2065
BisI GCNGC 4 cut(s) 202, 1371, 1647, 2394
BlsI GCNGC 4 cut(s) 203, 1372, 1648, 2395
Bme1390I CCNGG 3 cut(s) 242, 1837, 2346
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 8 cut(s) 239, 277, 1618, 2030, 2164, 2174, 2229, 2343
BmrFI CCNGG 3 cut(s) 242, 1837, 2346
BmsI GCATC 9 cut(s) 381, 568, 871, 894, 1267, 1414, 1513, 1854, 2266
BpiI GAAGAC 2 cut(s) 1483, 2438
BpmI CTGGAG 2 cut(s) 423, 813
Bpu14I TTCGAA 1 cut(s) 2308
BpuEI CTTGAG 2 cut(s) 992, 1058
BsaAI YACGTR 2 cut(s) 1190, 2093
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 1312
BsaJI CCNNGG 4 cut(s) 241, 320, 2176, 2345
BsaWI WCCGGW 1 cut(s) 1573
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 505, 2137, 2138
Bse1I ACTGG 5 cut(s) 23, 121, 499, 830, 1341
Bse3DI GCAATG 5 cut(s) 154, 858, 1145, 2188, 2432
BseBI CCWGG 3 cut(s) 242, 1837, 2346
BseDI CCNNGG 4 cut(s) 241, 320, 2176, 2345
BseGI GGATG 7 cut(s) 583, 1132, 1225, 1272, 1404, 1528, 2500
BseLI CCNNNNNNNGG 4 cut(s) 100, 505, 2137, 2138
BseMI GCAATG 5 cut(s) 154, 858, 1145, 2188, 2432
BseMII CTCAG 3 cut(s) 386, 1734, 2435
BseNI ACTGG 5 cut(s) 23, 121, 499, 830, 1341
BseRI GAGGAG 1 cut(s) 2246
BseSI GKGCMC 3 cut(s) 1147, 1450, 1753
BseXI GCAGC 4 cut(s) 213, 1357, 1633, 2405
BseYI CCCAGC 1 cut(s) 2032
BsgI GTGCAG 2 cut(s) 1189, 1216
BshFI GGCC 1 cut(s) 1968
BshNI GGYRCC 3 cut(s) 275, 1616, 2227
BsiHKAI GWGCWC 3 cut(s) 539, 1147, 1753
BsiSI CCGG 1 cut(s) 1574
BslFI GGGAC 1 cut(s) 1621
BslI CCNNNNNNNGG 4 cut(s) 100, 505, 2137, 2138
BsmAI GTCTC 3 cut(s) 162, 750, 1312
BsmFI GGGAC 1 cut(s) 1621
BsnI GGCC 1 cut(s) 1968
Bso31I GGTCTC 1 cut(s) 1312
Bsp119I TTCGAA 1 cut(s) 2308
Bsp1286I GDGCHC 4 cut(s) 539, 1147, 1450, 1753
Bsp1407I TGTACA 1 cut(s) 3
Bsp19I CCATGG 1 cut(s) 2176
BspACI CCGC 4 cut(s) 204, 261, 886, 2231
BspANI GGCC 1 cut(s) 1968
BspCNI CTCAG 3 cut(s) 387, 1735, 2434
BspHI TCATGA 2 cut(s) 1707, 1900
BspLI GGNNCC 8 cut(s) 239, 277, 1618, 2030, 2164, 2174, 2229, 2343
BspPI GGATC 6 cut(s) 232, 245, 1690, 1928, 2023, 2036
BspT104I TTCGAA 1 cut(s) 2308
BspT107I GGYRCC 3 cut(s) 275, 1616, 2227
BspTI CTTAAG 2 cut(s) 1409, 1877
BspTNI GGTCTC 1 cut(s) 1312
BsrDI GCAATG 5 cut(s) 154, 858, 1145, 2188, 2432
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 23, 121, 499, 830, 1341
BssECI CCNNGG 4 cut(s) 241, 320, 2176, 2345
BssNAI GTATAC 1 cut(s) 1159
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 320, 2176
Bst1107I GTATAC 1 cut(s) 1159
Bst2UI CCWGG 3 cut(s) 242, 1837, 2346
Bst4CI ACNGT 9 cut(s) 8, 552, 566, 1097, 1512, 1631, 2354, 2415, 2444
Bst6I CTCTTC 3 cut(s) 1572, 1812, 2193
BstACI GRCGYC 1 cut(s) 276
BstAFI CTTAAG 2 cut(s) 1409, 1877
BstAPI GCANNNNNTGC 1 cut(s) 1117
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 2 cut(s) 1190, 2093
BstBI TTCGAA 1 cut(s) 2308
BstDEI CTNAG 5 cut(s) 395, 1743, 1783, 2421, 2459
BstDSI CCRYGG 1 cut(s) 2176
BstF5I GGATG 7 cut(s) 583, 1132, 1225, 1272, 1404, 1528, 2500
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 3 cut(s) 278, 1651, 1979
BstMAI GTCTC 3 cut(s) 162, 750, 1312
BstMWI GCNNNNNNNGC 7 cut(s) 273, 761, 1117, 1367, 1655, 1748, 1974
BstNI CCWGG 3 cut(s) 242, 1837, 2346
BstNSI RCATGY 6 cut(s) 194, 289, 810, 1462, 1765, 2122
BstSCI CCNGG 3 cut(s) 240, 1835, 2344
BstSLI GKGCMC 3 cut(s) 1147, 1450, 1753
BstV1I GCAGC 4 cut(s) 213, 1357, 1633, 2405
BstV2I GAAGAC 2 cut(s) 1483, 2438
BstX2I RGATCY 7 cut(s) 237, 1588, 1695, 1786, 1920, 2028, 2065
BstYI RGATCY 7 cut(s) 237, 1588, 1695, 1786, 1920, 2028, 2065
BstZ17I GTATAC 1 cut(s) 1159
BsuRI GGCC 1 cut(s) 1968
BtgI CCRYGG 1 cut(s) 2176
BtsCI GGATG 7 cut(s) 583, 1132, 1225, 1272, 1404, 1528, 2500
BtsI GCAGTG 2 cut(s) 729, 1111
BtsIMutI CAGTG 5 cut(s) 729, 837, 1111, 1334, 1751
CciI TCATGA 2 cut(s) 1707, 1900
CfoI GCGC 3 cut(s) 278, 1651, 1979
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 4 cut(s) 4, 421, 780, 2350
CspCI CAANNNNNGTGG 1 cut(s) 2493
CviQI GTAC 4 cut(s) 4, 421, 780, 2350
DdeI CTNAG 5 cut(s) 395, 1743, 1783, 2421, 2459
DinI GGCGCC 1 cut(s) 277
DraIII CACNNNGTG 1 cut(s) 1117
EaeI YGGCCR 1 cut(s) 1966
Eam1104I CTCTTC 3 cut(s) 1572, 1812, 2193
EarI CTCTTC 3 cut(s) 1572, 1812, 2193
Ecl136II GAGCTC 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 320, 2176
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 1312
Eco32I GATATC 2 cut(s) 942, 1002
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 537
Eco57I CTGAAG 2 cut(s) 741, 2321
EcoICRI GAGCTC 1 cut(s) 537
EcoRI GAATTC 2 cut(s) 1324, 1992
EcoRII CCWGG 3 cut(s) 240, 1835, 2344
EcoRV GATATC 2 cut(s) 942, 1002
EcoT14I CCWWGG 2 cut(s) 320, 2176
EcoT22I ATGCAT 2 cut(s) 583, 1282
EcoT38I GRGCYC 1 cut(s) 539
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
ErhI CCWWGG 2 cut(s) 320, 2176
FaqI GGGAC 1 cut(s) 1621
FauI CCCGC 2 cut(s) 254, 893
FauNDI CATATG 1 cut(s) 2382
FblI GTMKAC 3 cut(s) 27, 175, 1158
Fnu4HI GCNGC 4 cut(s) 202, 1371, 1647, 2394
FokI GGATG 7 cut(s) 590, 1139, 1232, 1279, 1391, 1535, 2487
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 4 cut(s) 202, 1371, 1647, 2394
FspBI CTAG 5 cut(s) 920, 1064, 2037, 2453, 2481
GlaI GCGC 3 cut(s) 277, 1650, 1978
GluI GCNGC 4 cut(s) 202, 1371, 1647, 2394
GsaI CCCAGC 1 cut(s) 2036
GsuI CTGGAG 2 cut(s) 423, 813
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 1 cut(s) 1968
HapII CCGG 1 cut(s) 1574
HhaI GCGC 3 cut(s) 278, 1651, 1979
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 3 cut(s) 276, 1649, 1977
HinP1I GCGC 3 cut(s) 276, 1649, 1977
HindIII AAGCTT 2 cut(s) 141, 444
HpaII CCGG 1 cut(s) 1574
HphI GGTGA 3 cut(s) 402, 1930, 2399
Hpy188I TCNGA 7 cut(s) 760, 823, 880, 1377, 1516, 1732, 2424
Hpy188III TCNNGA 9 cut(s) 229, 402, 575, 992, 1473, 1708, 1901, 2208, 2453
HpyAV CCTTC 6 cut(s) 98, 1059, 1290, 1607, 2296, 2501
HpyCH4III ACNGT 9 cut(s) 8, 552, 566, 1097, 1512, 1631, 2354, 2415, 2444
HpyCH4IV ACGT 5 cut(s) 34, 728, 982, 1189, 2092
HpyF10VI GCNNNNNNNGC 7 cut(s) 273, 761, 1117, 1367, 1655, 1748, 1974
HpyF3I CTNAG 5 cut(s) 395, 1743, 1783, 2421, 2459
HpySE526I ACGT 5 cut(s) 34, 728, 982, 1189, 2092
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 3 cut(s) 276, 1649, 1977
KasI GGCGCC 1 cut(s) 275
LmnI GCTCC 7 cut(s) 264, 534, 542, 585, 2044, 2162, 2178
Lsp1109I GCAGC 4 cut(s) 213, 1357, 1633, 2405
LweI GCATC 9 cut(s) 381, 568, 871, 894, 1267, 1414, 1513, 1854, 2266
MaeI CTAG 5 cut(s) 920, 1064, 2037, 2453, 2481
MaeII ACGT 5 cut(s) 34, 728, 982, 1189, 2092
MaeIII GTNAC 7 cut(s) 356, 724, 922, 1097, 1299, 1936, 2405
MboII GAAGA 7 cut(s) 1488, 1589, 1829, 1834, 2210, 2438, 2441
MflI RGATCY 7 cut(s) 237, 1588, 1695, 1786, 1920, 2028, 2065
MhlI GDGCHC 4 cut(s) 539, 1147, 1450, 1753
MlsI TGGCCA 1 cut(s) 1968
MluNI TGGCCA 1 cut(s) 1968
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 4 cut(s) 164, 827, 1473, 2413
MmeI TCCRAC 7 cut(s) 190, 795, 801, 1514, 2022, 2336, 2519
Mox20I TGGCCA 1 cut(s) 1968
Mph1103I ATGCAT 2 cut(s) 583, 1282
MscI TGGCCA 1 cut(s) 1968
MslI CAYNNNNRTG 7 cut(s) 55, 189, 645, 1238, 2091, 2181, 2381
Msp20I TGGCCA 1 cut(s) 1968
MspA1I CMGCKG 2 cut(s) 204, 1646
MspCI CTTAAG 2 cut(s) 1409, 1877
MspI CCGG 1 cut(s) 1574
MspR9I CCNGG 3 cut(s) 242, 1837, 2346
MvaI CCWGG 3 cut(s) 242, 1837, 2346
MwoI GCNNNNNNNGC 7 cut(s) 273, 761, 1117, 1367, 1655, 1748, 1974
NarI GGCGCC 1 cut(s) 276
NcoI CCATGG 1 cut(s) 2176
NdeI CATATG 1 cut(s) 2382
NlaIV GGNNCC 8 cut(s) 239, 277, 1618, 2030, 2164, 2174, 2229, 2343
NmuCI GTSAC 4 cut(s) 724, 922, 1936, 2405
NsiI ATGCAT 2 cut(s) 583, 1282
NspI RCATGY 6 cut(s) 194, 289, 810, 1462, 1765, 2122
NspV TTCGAA 1 cut(s) 2308
PaeI GCATGC 1 cut(s) 2122
PagI TCATGA 2 cut(s) 1707, 1900
PciI ACATGT 4 cut(s) 190, 285, 1458, 1761
PfeI GAWTC 6 cut(s) 544, 824, 1022, 1864, 1883, 2135
PflMI CCANNNNNTGG 1 cut(s) 505
PfoI TCCNGGA 1 cut(s) 1835
PkrI GCNGC 4 cut(s) 203, 1372, 1648, 2395
PleI GAGTC 4 cut(s) 163, 826, 1473, 2412
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 4 cut(s) 163, 826, 1473, 2412
Ppu21I YACGTR 2 cut(s) 1190, 2093
PscI ACATGT 4 cut(s) 190, 285, 1458, 1761
PsiI TTATAA 1 cut(s) 2475
Psp124BI GAGCTC 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 240, 1835, 2344
PspFI CCCAGC 1 cut(s) 2032
PspGI CCWGG 3 cut(s) 240, 1835, 2344
PspN4I GGNNCC 8 cut(s) 239, 277, 1618, 2030, 2164, 2174, 2229, 2343
PspPI GGNCC 1 cut(s) 60
PsuI RGATCY 7 cut(s) 237, 1588, 1695, 1786, 1920, 2028, 2065
PvuII CAGCTG 1 cut(s) 1646
RsaI GTAC 4 cut(s) 5, 422, 781, 2351
RsaNI GTAC 4 cut(s) 4, 421, 780, 2350
RseI CAYNNNNRTG 7 cut(s) 55, 189, 645, 1238, 2091, 2181, 2381
SacI GAGCTC 1 cut(s) 539
SatI GCNGC 4 cut(s) 202, 1371, 1647, 2394
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 4 cut(s) 164, 827, 1473, 2413
ScrFI CCNGG 3 cut(s) 242, 1837, 2346
SduI GDGCHC 4 cut(s) 539, 1147, 1450, 1753
SfaNI GCATC 9 cut(s) 381, 568, 871, 894, 1267, 1414, 1513, 1854, 2266
SfoI GGCGCC 1 cut(s) 277
SfuI TTCGAA 1 cut(s) 2308
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 7 cut(s) 55, 189, 645, 1238, 2091, 2181, 2381
SmlI CTYRAG 4 cut(s) 971, 1073, 1409, 1877
SmoI CTYRAG 4 cut(s) 971, 1073, 1409, 1877
SphI GCATGC 1 cut(s) 2122
SsiI CCGC 4 cut(s) 204, 261, 886, 2231
SspDI GGCGCC 1 cut(s) 275
SspMI CTAG 5 cut(s) 920, 1064, 2037, 2453, 2481
SstI GAGCTC 1 cut(s) 539
StyD4I CCNGG 3 cut(s) 240, 1835, 2344
StyI CCWWGG 2 cut(s) 320, 2176
TaaI ACNGT 9 cut(s) 8, 552, 566, 1097, 1512, 1631, 2354, 2415, 2444
TaiI ACGT 5 cut(s) 37, 731, 985, 1192, 2095
TaqI TCGA 8 cut(s) 329, 547, 910, 1025, 1482, 1495, 1541, 2308
TaqII GACCGA 1 cut(s) 1336
TatI WGTACW 2 cut(s) 3, 420
TfiI GAWTC 6 cut(s) 544, 824, 1022, 1864, 1883, 2135
TscAI CASTG 5 cut(s) 729, 837, 1118, 1341, 1758
TseFI GTSAC 4 cut(s) 724, 922, 1936, 2405
TseI GCWGC 4 cut(s) 201, 1370, 1646, 2393
Tsp45I GTSAC 4 cut(s) 724, 922, 1936, 2405
TspGWI ACGGA 3 cut(s) 1232, 1625, 1994
TspRI CASTG 5 cut(s) 729, 837, 1118, 1341, 1758
Van91I CCANNNNNTGG 1 cut(s) 505
Vha464I CTTAAG 2 cut(s) 1409, 1877
VneI GTGCAC 2 cut(s) 1143, 1749
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 7 cut(s) 1089, 1194, 1287, 1324, 1379, 1992, 2236
XbaI TCTAGA 1 cut(s) 2452
XceI RCATGY 6 cut(s) 194, 289, 810, 1462, 1765, 2122
XmiI GTMKAC 3 cut(s) 27, 175, 1158
XspI CTAG 5 cut(s) 920, 1064, 2037, 2453, 2481
Zsp2I ATGCAT 2 cut(s) 583, 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.