Prupe.1G321500_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016027
Physical Location & Seq
Forward (+)
30792335 .. 30794243
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G321500.1

Sequence Viewer

Length: 1125 bp
ATGGAGAAAGTGCAAGTGGTCTTGATCATGATGGTTAGACTAGTTTTCTTTCTTGCATCTATATTCTTATCTGTTTCCTCGGCAAAACATTCTCCGTCCAGTGTTAACGTTGTTTCTGGGATCTTCATCTTCGGAGACTCAATCTTCGACGCCGGAAACAACCACTTCAACAAGAACAGCACCGCTCAAGCAGATTTTCCCCCCTACGGCTCAACCTTCTTCCACTACCCAACTGGCAGGTTCACTAATGGCAGAACTGTTGCTGACTTCATTTCACAATTCATGGGCATTGATCTTCAAAAACCATATCTTGAGGCACGGATTGAAGTTCTCAATGGAAGCCTCAAAGATTATCCCTCCAATGGCATTAACTTTGCCAGTGCTGGCAGTGGTGTTTTGCAGGCAACAAATCGCGATTTGGGCGTGACCTCACTCCAGGAACAATTACGACAATTCCAAACACTGGTGGAGCAAAACCAGGTTAACAAAACCCTAGTCCAAAACTCCATCTTCTTGTTCGAGTCTGGTTCCAACGACATCTTCAGCTACTTCATCCCAATTGACCCTCCAAAACTTGACCCCGACGCCTATGTGCAAGCCATGCTAACGCAAGTCTCAAAATTCTTGGACCAAATTTACAAACTCGGCGCTCGCCGAGTCTCCGTATTTTCACTGGGACCTGTTGGGTGTGTCCCAGCCAGGGCCCTCTTGCCAGGCACACCCATTGATCGATGTTACGGGAAGATGAATGCTATGGTCAACAAATTCAACAAGGGTCTAGAAAGCTTGGTCAACGATATGCCTTCTAAGCACCGCGGTGTGCTCAGTGTTTATGGTGCAGTCTATGACATTGTTCAACACTTTCGAGCCACTCCTACACGTTACCGGTTTTCGGACGTATCGAGTGCATGTTGCGGGGAAGGTACTCTAGGGGGAATGATACAGTGCGGCAAGGAGGGCTACGGGCTTTGTCCAAATCCCAATGAGTTTCTGTTCTGGGATTATTTCCATCCGTCGGAGCGTGCTTACAAGCTCTTATCCAAGGCCTTGTGGGCTGGCAAGAAATCTCGAATTAGACCTCTTAATTTGAAGACTCTAGTCAACATTAGCCTCCCTCATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

41.48

Weight (kDa)

9.1

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
AccB7I CCANNNNNTGG 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 185
AccII CGCG 2 cut(s) 414, 816
AciI CCGC 5 cut(s) 183, 814, 816, 915, 948
AclI AACGTT 1 cut(s) 108
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 3 cut(s) 620, 633, 764
AcuI CTGAAG 1 cut(s) 526
AcyI GRCGYC 2 cut(s) 150, 585
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 6 cut(s) 206, 362, 463, 700, 892, 1015
AflIII ACRYGT 1 cut(s) 878
AgeI ACCGGT 1 cut(s) 885
AgsI TTSAA 6 cut(s) 169, 299, 326, 769, 857, 1090
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 4 cut(s) 435, 477, 698, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AleI CACNNNNGTG 1 cut(s) 816
AluBI AGCT 3 cut(s) 546, 786, 1033
AluI AGCT 3 cut(s) 546, 786, 1033
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 3 cut(s) 129, 619, 664
AlwI GGATC 1 cut(s) 128
AoxI GGCC 2 cut(s) 702, 1044
ApaI GGGCCC 1 cut(s) 706
ApoI RAATTY 3 cut(s) 620, 633, 764
ArsI GACNNNNNNTTYG 4 cut(s) 128, 160, 480, 512
AsiGI ACCGGT 1 cut(s) 885
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 4 cut(s) 628, 677, 702, 703
AvaII GGWCC 2 cut(s) 628, 677
BaeGI GKGCMC 1 cut(s) 706
BanII GRGCYC 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 1097
Bbv12I GWGCWC 1 cut(s) 825
BccI CCATC 3 cut(s) 25, 515, 1017
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 4 cut(s) 437, 479, 700, 714
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 3 cut(s) 129, 619, 664
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 5 cut(s) 41, 494, 779, 929, 1097
BfoI RGCGCY 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 228
BglI GCCNNNNNGGC 1 cut(s) 1052
BisI GCNGC 1 cut(s) 949
BlsI GCNGC 1 cut(s) 950
Bme1390I CCNGG 4 cut(s) 437, 479, 700, 714
Bme18I GGWCC 2 cut(s) 628, 677
BmgT120I GGNCC 4 cut(s) 628, 677, 702, 703
BmiI GGNNCC 3 cut(s) 529, 678, 704
BmrFI CCNGG 4 cut(s) 437, 479, 700, 714
BmrI ACTGGG 1 cut(s) 683
BmsI GCATC 1 cut(s) 65
BmuI ACTGGG 1 cut(s) 683
BoxI GACNNNNGTC 1 cut(s) 1097
BpiI GAAGAC 1 cut(s) 1097
BpmI CTGGAG 1 cut(s) 419
BpuEI CTTGAG 2 cut(s) 171, 332
Bsa29I ATCGAT 1 cut(s) 730
BsaBI GATNNNNATC 1 cut(s) 125
BsaHI GRCGYC 2 cut(s) 150, 585
BsaJI CCNNGG 4 cut(s) 78, 699, 814, 1041
BsaWI WCCGGW 1 cut(s) 885
Bsc4I CCNNNNNNNGG 6 cut(s) 206, 362, 463, 700, 892, 1015
Bse118I RCCGGY 1 cut(s) 885
Bse1I ACTGG 5 cut(s) 99, 238, 378, 468, 678
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 4 cut(s) 437, 479, 700, 714
BseCI ATCGAT 1 cut(s) 730
BseDI CCNNGG 4 cut(s) 78, 699, 814, 1041
BseGI GGATG 2 cut(s) 552, 1009
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 6 cut(s) 206, 362, 463, 700, 892, 1015
BseMII CTCAG 1 cut(s) 838
BseNI ACTGG 5 cut(s) 99, 238, 378, 468, 678
BseSI GKGCMC 1 cut(s) 706
BseYI CCCAGC 1 cut(s) 694
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 2 cut(s) 414, 816
BshFI GGCC 2 cut(s) 704, 1046
BshTI ACCGGT 1 cut(s) 885
BshVI ATCGAT 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 825
BsiSI CCGG 2 cut(s) 153, 886
BslFI GGGAC 2 cut(s) 677, 690
BslI CCNNNNNNNGG 6 cut(s) 206, 362, 463, 700, 892, 1015
BsmAI GTCTC 3 cut(s) 129, 619, 664
BsmFI GGGAC 2 cut(s) 677, 690
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 2 cut(s) 704, 1046
Bsp120I GGGCCC 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 706, 825
Bsp143I GATC 4 cut(s) 24, 120, 292, 727
Bsp68I TCGCGA 1 cut(s) 414
BspACI CCGC 5 cut(s) 183, 814, 816, 915, 948
BspANI GGCC 2 cut(s) 704, 1046
BspCNI CTCAG 1 cut(s) 837
BspDI ATCGAT 1 cut(s) 730
BspFNI CGCG 2 cut(s) 414, 816
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 529, 678, 704
BspMI ACCTGC 1 cut(s) 228
BspPI GGATC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 885
BsrI ACTGG 5 cut(s) 99, 238, 378, 468, 678
BssAI RCCGGY 1 cut(s) 885
BssECI CCNNGG 4 cut(s) 78, 699, 814, 1041
BssMI GATC 4 cut(s) 24, 120, 292, 727
BssNI GRCGYC 2 cut(s) 150, 585
BssT1I CCWWGG 1 cut(s) 1041
Bst2UI CCWGG 4 cut(s) 437, 479, 700, 714
Bst4CI ACNGT 2 cut(s) 259, 945
BstACI GRCGYC 2 cut(s) 150, 585
BstAPI GCANNNNNTGC 1 cut(s) 601
BstC8I GCNNGC 6 cut(s) 385, 402, 597, 652, 1023, 1057
BstDEI CTNAG 2 cut(s) 807, 824
BstDSI CCRYGG 1 cut(s) 814
BstF5I GGATG 2 cut(s) 552, 1009
BstFNI CGCG 2 cut(s) 414, 816
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 4 cut(s) 27, 123, 295, 730
BstMAI GTCTC 3 cut(s) 129, 619, 664
BstMBI GATC 4 cut(s) 24, 120, 292, 727
BstMWI GCNNNNNNNGC 5 cut(s) 420, 601, 808, 957, 1052
BstNI CCWGG 4 cut(s) 437, 479, 700, 714
BstNSI RCATGY 1 cut(s) 912
BstPAI GACNNNNGTC 1 cut(s) 1097
BstSCI CCNGG 4 cut(s) 435, 477, 698, 712
BstSLI GKGCMC 1 cut(s) 706
BstUI CGCG 2 cut(s) 414, 816
BstV2I GAAGAC 1 cut(s) 1097
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 730
BsuRI GGCC 2 cut(s) 704, 1046
BsuTUI ATCGAT 1 cut(s) 730
BtgI CCRYGG 1 cut(s) 814
BtsCI GGATG 2 cut(s) 552, 1009
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 7 cut(s) 106, 385, 394, 461, 671, 832, 950
BtuMI TCGCGA 1 cut(s) 414
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 6 cut(s) 385, 402, 597, 652, 1023, 1057
CciI TCATGA 1 cut(s) 27
CfoI GCGC 1 cut(s) 650
Cfr10I RCCGGY 1 cut(s) 885
Cfr13I GGNCC 4 cut(s) 628, 677, 702, 703
Cfr42I CCGCGG 1 cut(s) 817
ClaI ATCGAT 1 cut(s) 730
CseI GACGC 2 cut(s) 158, 593
CsiI ACCWGGT 1 cut(s) 477
Csp6I GTAC 1 cut(s) 924
CspAI ACCGGT 1 cut(s) 885
CviAII CATG 5 cut(s) 28, 283, 601, 909, 1118
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 2 cut(s) 807, 824
DpnI GATC 4 cut(s) 26, 122, 294, 729
DpnII GATC 4 cut(s) 24, 120, 292, 727
Eco130I CCWWGG 1 cut(s) 1041
Eco147I AGGCCT 1 cut(s) 1046
Eco24I GRGCYC 1 cut(s) 706
Eco47I GGWCC 2 cut(s) 628, 677
Eco57I CTGAAG 1 cut(s) 526
EcoO109I RGGNCCY 3 cut(s) 677, 702, 703
EcoRII CCWGG 4 cut(s) 435, 477, 698, 712
EcoT14I CCWWGG 1 cut(s) 1041
EcoT38I GRGCYC 1 cut(s) 706
ErhI CCWWGG 1 cut(s) 1041
FaeI CATG 5 cut(s) 31, 286, 604, 912, 1121
FaqI GGGAC 2 cut(s) 677, 690
FatI CATG 5 cut(s) 27, 282, 600, 908, 1117
FauI CCCGC 1 cut(s) 908
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 949
FokI GGATG 2 cut(s) 539, 996
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 1 cut(s) 949
FspBI CTAG 5 cut(s) 41, 494, 779, 929, 1097
GlaI GCGC 1 cut(s) 649
GluI GCNGC 1 cut(s) 949
GsaI CCCAGC 1 cut(s) 698
GsuI CTGGAG 1 cut(s) 419
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 2 cut(s) 704, 1046
HapII CCGG 2 cut(s) 153, 886
HgaI GACGC 2 cut(s) 158, 593
HhaI GCGC 1 cut(s) 650
Hin1I GRCGYC 2 cut(s) 150, 585
Hin1II CATG 5 cut(s) 31, 286, 604, 912, 1121
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HincII GTYRAC 5 cut(s) 106, 484, 760, 793, 1102
HindII GTYRAC 5 cut(s) 106, 484, 760, 793, 1102
HindIII AAGCTT 1 cut(s) 784
HinfI GANTC 4 cut(s) 137, 521, 657, 1093
HpaI GTTAAC 2 cut(s) 106, 484
HpaII CCGG 2 cut(s) 153, 886
Hpy166II GTNNAC 6 cut(s) 106, 243, 484, 760, 793, 1102
Hpy188I TCNGA 3 cut(s) 134, 895, 1018
Hpy188III TCNNGA 6 cut(s) 22, 28, 311, 413, 779, 1068
Hpy8I GTNNAC 6 cut(s) 106, 243, 484, 760, 793, 1102
Hpy99I CGWCG 3 cut(s) 152, 587, 1018
HpyAV CCTTC 3 cut(s) 226, 813, 914
HpyCH4III ACNGT 2 cut(s) 259, 945
HpyCH4IV ACGT 3 cut(s) 108, 880, 897
HpyCH4V TGCA 6 cut(s) 13, 56, 400, 595, 839, 908
HpyF10VI GCNNNNNNNGC 5 cut(s) 420, 601, 808, 957, 1052
HpyF3I CTNAG 2 cut(s) 807, 824
HpySE526I ACGT 3 cut(s) 108, 880, 897
Hsp92I GRCGYC 2 cut(s) 150, 585
Hsp92II CATG 5 cut(s) 31, 286, 604, 912, 1121
HspAI GCGC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 24
KspAI GTTAAC 2 cut(s) 106, 484
KspI CCGCGG 1 cut(s) 817
Kzo9I GATC 4 cut(s) 24, 120, 292, 727
LmnI GCTCC 2 cut(s) 469, 1018
LweI GCATC 1 cut(s) 65
MabI ACCWGGT 1 cut(s) 477
MaeI CTAG 5 cut(s) 41, 494, 779, 929, 1097
MaeII ACGT 3 cut(s) 108, 880, 897
MaeIII GTNAC 3 cut(s) 424, 734, 881
MalI GATC 4 cut(s) 26, 122, 294, 729
MbiI CCGCTC 1 cut(s) 185
MboI GATC 4 cut(s) 24, 120, 292, 727
MboII GAAGA 9 cut(s) 115, 121, 136, 211, 287, 502, 532, 754, 1102
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 2 cut(s) 706, 825
MluCI AATT 9 cut(s) 278, 443, 452, 558, 620, 633, 764, 1071, 1084
MlyI GAGTC 4 cut(s) 131, 530, 666, 1087
MmeI TCCRAC 2 cut(s) 555, 996
MseI TTAA 4 cut(s) 105, 369, 483, 1083
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 816
MspI CCGG 2 cut(s) 153, 886
MspR9I CCNGG 4 cut(s) 437, 479, 700, 714
MunI CAATTG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 4 cut(s) 437, 479, 700, 714
MvnI CGCG 2 cut(s) 414, 816
MwoI GCNNNNNNNGC 5 cut(s) 420, 601, 808, 957, 1052
NdeII GATC 4 cut(s) 24, 120, 292, 727
NlaIII CATG 5 cut(s) 31, 286, 604, 912, 1121
NlaIV GGNNCC 3 cut(s) 529, 678, 704
NmeAIII GCCGAG 3 cut(s) 59, 624, 680
NmuCI GTSAC 1 cut(s) 424
NruI TCGCGA 1 cut(s) 414
NspI RCATGY 1 cut(s) 912
OliI CACNNNNGTG 1 cut(s) 816
PagI TCATGA 1 cut(s) 27
PceI AGGCCT 1 cut(s) 1046
PcsI WCGNNNNNNNCGW 1 cut(s) 899
PctI GAATGC 1 cut(s) 754
PflMI CCANNNNNTGG 1 cut(s) 463
PfoI TCCNGGA 1 cut(s) 435
PinAI ACCGGT 1 cut(s) 885
PkrI GCNGC 1 cut(s) 950
PleI GAGTC 4 cut(s) 131, 529, 665, 1087
PpsI GAGTC 4 cut(s) 131, 529, 665, 1087
PpuMI RGGWCCY 1 cut(s) 677
PshAI GACNNNNGTC 1 cut(s) 1097
Psp1406I AACGTT 1 cut(s) 108
Psp5II RGGWCCY 1 cut(s) 677
Psp6I CCWGG 4 cut(s) 435, 477, 698, 712
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 4 cut(s) 435, 477, 698, 712
PspN4I GGNNCC 3 cut(s) 529, 678, 704
PspOMI GGGCCC 1 cut(s) 702
PspPI GGNCC 4 cut(s) 628, 677, 702, 703
PspPPI RGGWCCY 1 cut(s) 677
PsuI RGATCY 1 cut(s) 120
RruI TCGCGA 1 cut(s) 414
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 816
SacII CCGCGG 1 cut(s) 817
SaqAI TTAA 4 cut(s) 105, 369, 483, 1083
SatI GCNGC 1 cut(s) 949
Sau3AI GATC 4 cut(s) 24, 120, 292, 727
Sau96I GGNCC 4 cut(s) 628, 677, 702, 703
SchI GAGTC 4 cut(s) 131, 530, 666, 1087
ScrFI CCNGG 4 cut(s) 437, 479, 700, 714
SduI GDGCHC 2 cut(s) 706, 825
SexAI ACCWGGT 1 cut(s) 477
SfaNI GCATC 1 cut(s) 65
Sfr303I CCGCGG 1 cut(s) 817
SgrBI CCGCGG 1 cut(s) 817
SinI GGWCC 2 cut(s) 628, 677
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 2 cut(s) 186, 311
SmoI CTYRAG 2 cut(s) 186, 311
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 9 cut(s) 278, 443, 452, 558, 620, 633, 764, 1071, 1084
SseBI AGGCCT 1 cut(s) 1046
SsiI CCGC 5 cut(s) 183, 814, 816, 915, 948
SspMI CTAG 5 cut(s) 41, 494, 779, 929, 1097
StuI AGGCCT 1 cut(s) 1046
StyD4I CCNGG 4 cut(s) 435, 477, 698, 712
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 2 cut(s) 259, 945
TaiI ACGT 3 cut(s) 111, 883, 900
TaqI TCGA 6 cut(s) 147, 519, 730, 865, 902, 1069
TasI AATT 9 cut(s) 278, 443, 452, 558, 620, 633, 764, 1071, 1084
TauI GCSGC 1 cut(s) 951
Tru1I TTAA 4 cut(s) 105, 369, 483, 1083
Tru9I TTAA 4 cut(s) 105, 369, 483, 1083
TscAI CASTG 7 cut(s) 106, 385, 394, 468, 678, 832, 950
TseFI GTSAC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 5 cut(s) 115, 259, 271, 541, 761
TspGWI ACGGA 4 cut(s) 84, 334, 652, 1002
TspRI CASTG 7 cut(s) 106, 385, 394, 468, 678, 832, 950
Van91I CCANNNNNTGG 1 cut(s) 463
VpaK11BI GGWCC 2 cut(s) 628, 677
XapI RAATTY 3 cut(s) 620, 633, 764
XbaI TCTAGA 1 cut(s) 778
XceI RCATGY 1 cut(s) 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 5 cut(s) 41, 494, 779, 929, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.