Rh4BG333600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016027
Physical Location & Seq
Reverse (-)
49756118 .. 49763210
7093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG333600.1

Sequence Viewer

Length: 1200 bp
ATGGAAATATCTGAGTCGTTGTTGGTCGACGATTCAGACAAAATCAAGGCCATTGAGGACGACCAAGTGCCAAAATGGAAGGAGCAGCACCGTCATAGGGCTGGTGGTCAGCGTCGTGCTGGGGTCCCTGTTATGCAATATCACCCACAAGCTGAATCTGATGATTCATCTCTAATATCCACTGCTGTTTGTGGGATCTTCACTTTCGGAGACTCCATCTTCGACGCTGGAAACAATCACTTCAACAAGAACTGCACTGTTCAAGCAGATTTCCCACCGTATGGCTCAAGCTTCTTCCACTACCCGACTGGCAGGTTCACTAATGGCAGAACTGTTGCTGATTTCATCTCACAATTCATCGGCATTCCTCTCCAAAAACCATATTATGAGGTACAAATTGAAGCTATGACTGGAAGCCGGAAAGGTTATCCTTCCAATGGCATTAACTTTGCTAGTGCCGGCAGCGGTGTTTTGCAAGGAACAAATAAAAATTTGGGAGTCACCTCACTCCAAGAACAATTACAACAATTCCAGACACTAGTGGAGCAAAACCACATTGGCAAAACTCTAGTTCAGAAATCCTTCTTCTTCTTCGAGTCTGGTTCCAACGACATCTTCAACTACTTTCTTCCATTTGCTCCCCCAACACTAGACCCGGATGCCTATGTCCAAGCCATGCTAACCCAAGTCACAAAATTCATAGACCAAATCTACATGCTTGGCACTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGCTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAATCGATGTTTCGGAAAGATGAACTTGATGGTTAAGAAGTACAACAAGGGACTGGAATGTTTGGTCAATGATATGCCTATCAAGTACCCCGGGGTGGTGGGTGTTTATGGAGAAACCTATGACATTGTTCAGCGATTTCGAGCCATTCCTACACGTTACCATTTTTCAGACGTATCGAGTGCATGTTGCGGCGGAGGTACTCTAGGAGGATTGCTGCAATGTGGGAAGGAGGGATACAAGACATGTGCAAACCCTAATGAGTTCTTATTCTGGGATTACTTCCATCCATCAGAGCATGCTTACAGGCTCATATCCAAGGCCTTGTGGGGTGGAAAGAAATATCGAATTAGACCACTTAATTTGAAGGCTCTAGCCAACATCACCCTCACTCGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

399

Amino Acids

44.44

Weight (kDa)

8.71

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 66 - 375 3.1e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 281
AccI GTMKAC 1 cut(s) 27
AciI CCGC 3 cut(s) 465, 990, 993
AclWI GGATC 1 cut(s) 203
AcsI RAATTY 2 cut(s) 490, 695
AfaI GTAC 4 cut(s) 393, 842, 887, 1000
AfiI CCNNNNNNNGG 4 cut(s) 97, 281, 437, 895
AflIII ACRYGT 2 cut(s) 953, 1043
AgsI TTSAA 5 cut(s) 244, 263, 401, 619, 1165
AhlI ACTAGT 1 cut(s) 538
AjnI CCWGG 2 cut(s) 773, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AluBI AGCT 3 cut(s) 152, 291, 404
AluI AGCT 3 cut(s) 152, 291, 404
Alw21I GWGCWC 2 cut(s) 769, 796
Alw26I GTCTC 1 cut(s) 204
Alw44I GTGCAC 1 cut(s) 792
AlwI GGATC 1 cut(s) 203
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 3 cut(s) 48, 752, 1119
ApaLI GTGCAC 1 cut(s) 792
ApeKI GCWGC 3 cut(s) 85, 462, 1015
ApoI RAATTY 2 cut(s) 490, 695
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 581
AspS9I GGNCC 2 cut(s) 124, 752
AsuC2I CCSGG 3 cut(s) 656, 891, 892
AsuHPI GGTGA 3 cut(s) 134, 493, 1174
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 1 cut(s) 124
BaeGI GKGCMC 2 cut(s) 781, 796
BarI GAAGNNNNNNTAC 2 cut(s) 1019, 1051
BauI CACGAG 1 cut(s) 1191
Bbv12I GWGCWC 2 cut(s) 769, 796
BbvI GCAGC 3 cut(s) 97, 474, 1002
BccI CCATC 4 cut(s) 224, 823, 1092, 1096
BciT130I CCWGG 2 cut(s) 775, 789
BciVI GTATCC 1 cut(s) 1028
BcnI CCSGG 3 cut(s) 656, 891, 892
BcoDI GTCTC 1 cut(s) 204
BcuI ACTAGT 1 cut(s) 538
BfaI CTAG 7 cut(s) 453, 539, 569, 650, 749, 1004, 1172
BfuAI ACCTGC 1 cut(s) 303
BfuI GTATCC 1 cut(s) 1028
BisI GCNGC 4 cut(s) 86, 463, 991, 1016
BlsI GCNGC 4 cut(s) 87, 464, 992, 1017
Bme1390I CCNGG 5 cut(s) 656, 775, 789, 891, 892
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 890
BmgT120I GGNCC 2 cut(s) 124, 752
BmiI GGNNCC 3 cut(s) 125, 126, 604
BmrFI CCNGG 5 cut(s) 656, 775, 789, 891, 892
BmsI GCATC 1 cut(s) 649
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 3 cut(s) 656, 891, 892
Bsa29I ATCGAT 1 cut(s) 805
BsaJI CCNNGG 5 cut(s) 774, 889, 890, 891, 1116
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 281, 437, 895
Bse118I RCCGGY 1 cut(s) 458
Bse1I ACTGG 3 cut(s) 313, 415, 858
Bse3DI GCAATG 1 cut(s) 1025
BseBI CCWGG 2 cut(s) 775, 789
BseCI ATCGAT 1 cut(s) 805
BseDI CCNNGG 5 cut(s) 774, 889, 890, 891, 1116
BseGI GGATG 2 cut(s) 664, 1084
BseLI CCNNNNNNNGG 4 cut(s) 97, 281, 437, 895
BseMI GCAATG 1 cut(s) 1025
BseNI ACTGG 3 cut(s) 313, 415, 858
BseSI GKGCMC 2 cut(s) 781, 796
BseXI GCAGC 3 cut(s) 97, 474, 1002
BseYI CCCAGC 1 cut(s) 119
BsgI GTGCAG 1 cut(s) 238
BshFI GGCC 3 cut(s) 50, 754, 1121
BshVI ATCGAT 1 cut(s) 805
BsiHKAI GWGCWC 2 cut(s) 769, 796
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 4 cut(s) 418, 459, 656, 891
BslFI GGGAC 2 cut(s) 110, 864
BslI CCNNNNNNNGG 4 cut(s) 97, 281, 437, 895
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 2 cut(s) 110, 864
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 3 cut(s) 50, 754, 1121
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 3 cut(s) 769, 781, 796
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 3 cut(s) 465, 990, 993
BspANI GGCC 3 cut(s) 50, 754, 1121
BspCNI CTCAG 1 cut(s) 4
BspDI ATCGAT 1 cut(s) 805
BspLI GGNNCC 3 cut(s) 125, 126, 604
BspMI ACCTGC 1 cut(s) 303
BspPI GGATC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 1025
BsrFI RCCGGY 1 cut(s) 458
BsrI ACTGG 3 cut(s) 313, 415, 858
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 5 cut(s) 774, 889, 890, 891, 1116
BssMI GATC 1 cut(s) 195
BssSI CACGAG 1 cut(s) 1191
BssT1I CCWWGG 1 cut(s) 1116
Bst2BI CACGAG 1 cut(s) 1191
Bst2UI CCWGG 2 cut(s) 775, 789
Bst4CI ACNGT 4 cut(s) 92, 259, 279, 334
BstC8I GCNNGC 2 cut(s) 460, 1098
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 664, 1084
BstKTI GATC 1 cut(s) 198
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 195
BstNI CCWGG 2 cut(s) 775, 789
BstNSI RCATGY 4 cut(s) 718, 987, 1047, 1100
BstSCI CCNGG 5 cut(s) 654, 773, 787, 889, 890
BstSLI GKGCMC 2 cut(s) 781, 796
BstV1I GCAGC 3 cut(s) 97, 474, 1002
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 805
BsuI GTATCC 1 cut(s) 1028
BsuRI GGCC 3 cut(s) 50, 754, 1121
BsuTUI ATCGAT 1 cut(s) 805
BtsCI GGATG 2 cut(s) 664, 1084
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 180, 255
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 460, 1098
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 124, 752
Cfr9I CCCGGG 1 cut(s) 890
ClaI ATCGAT 1 cut(s) 805
CseI GACGC 2 cut(s) 101, 233
Csp6I GTAC 4 cut(s) 392, 841, 886, 999
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviAII CATG 5 cut(s) 676, 715, 984, 1044, 1097
CviQI GTAC 4 cut(s) 392, 841, 886, 999
DdeI CTNAG 1 cut(s) 12
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 1008
Eco130I CCWWGG 1 cut(s) 1116
Eco147I AGGCCT 1 cut(s) 1121
Eco47I GGWCC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 890
EcoO109I RGGNCCY 2 cut(s) 124, 752
EcoRII CCWGG 2 cut(s) 773, 787
EcoT14I CCWWGG 1 cut(s) 1116
ErhI CCWWGG 1 cut(s) 1116
FaeI CATG 5 cut(s) 679, 718, 987, 1047, 1100
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 2 cut(s) 110, 864
FatI CATG 5 cut(s) 675, 714, 983, 1043, 1096
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 4 cut(s) 86, 463, 991, 1016
FokI GGATG 2 cut(s) 671, 1071
Fsp4HI GCNGC 4 cut(s) 86, 463, 991, 1016
FspBI CTAG 7 cut(s) 453, 539, 569, 650, 749, 1004, 1172
GluI GCNGC 4 cut(s) 86, 463, 991, 1016
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 3 cut(s) 50, 754, 1121
HapII CCGG 4 cut(s) 418, 459, 656, 891
HgaI GACGC 2 cut(s) 101, 233
Hin1II CATG 5 cut(s) 679, 718, 987, 1047, 1100
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 289
HinfI GANTC 7 cut(s) 14, 32, 155, 164, 212, 498, 596
HpaII CCGG 4 cut(s) 418, 459, 656, 891
HphI GGTGA 3 cut(s) 134, 493, 1174
Hpy166II GTNNAC 3 cut(s) 28, 318, 794
Hpy188I TCNGA 8 cut(s) 13, 37, 160, 209, 576, 815, 970, 1093
Hpy188III TCNNGA 1 cut(s) 532
Hpy8I GTNNAC 3 cut(s) 28, 318, 794
Hpy99I CGWCG 3 cut(s) 32, 117, 227
HpyAV CCTTC 5 cut(s) 73, 441, 592, 1021, 1159
HpyCH4III ACNGT 4 cut(s) 92, 259, 279, 334
HpyCH4IV ACGT 2 cut(s) 955, 972
HpyCH4V TGCA 7 cut(s) 136, 255, 475, 794, 983, 1018, 1049
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 955, 972
Hsp92II CATG 5 cut(s) 679, 718, 987, 1047, 1100
KflI GGGWCCC 1 cut(s) 124
KroI GCCGGC 1 cut(s) 458
KroNI GCCGGC 1 cut(s) 460
Kzo9I GATC 1 cut(s) 195
LmnI GCTCC 4 cut(s) 82, 544, 643, 772
Lsp1109I GCAGC 3 cut(s) 97, 474, 1002
LweI GCATC 1 cut(s) 649
MaeI CTAG 7 cut(s) 453, 539, 569, 650, 749, 1004, 1172
MaeII ACGT 2 cut(s) 955, 972
MaeIII GTNAC 3 cut(s) 499, 688, 956
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MboII GAAGA 8 cut(s) 190, 211, 286, 577, 580, 583, 607, 620
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 3 cut(s) 769, 781, 796
MluCI AATT 9 cut(s) 353, 396, 490, 518, 527, 695, 732, 1146, 1159
MlyI GAGTC 4 cut(s) 23, 206, 507, 605
MmeI TCCRAC 1 cut(s) 630
MnlI CCTC 8 cut(s) 49, 378, 382, 514, 989, 1001, 1024, 1196
MroNI GCCGGC 1 cut(s) 458
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 4 cut(s) 444, 801, 834, 1158
MspA1I CMGCKG 1 cut(s) 465
MspI CCGG 4 cut(s) 418, 459, 656, 891
MspR9I CCNGG 5 cut(s) 656, 775, 789, 891, 892
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 2 cut(s) 775, 789
NaeI GCCGGC 1 cut(s) 460
NciI CCSGG 3 cut(s) 656, 891, 892
NdeII GATC 1 cut(s) 195
NgoMIV GCCGGC 1 cut(s) 458
NlaIII CATG 5 cut(s) 679, 718, 987, 1047, 1100
NlaIV GGNNCC 3 cut(s) 125, 126, 604
NmuCI GTSAC 2 cut(s) 499, 688
NspI RCATGY 4 cut(s) 718, 987, 1047, 1100
PaeI GCATGC 1 cut(s) 1100
PceI AGGCCT 1 cut(s) 1121
PciI ACATGT 1 cut(s) 1043
PctI GAATGC 1 cut(s) 363
PdiI GCCGGC 1 cut(s) 460
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 32, 155, 164
PflMI CCANNNNNTGG 1 cut(s) 281
PkrI GCNGC 4 cut(s) 87, 464, 992, 1017
PleI GAGTC 4 cut(s) 22, 206, 506, 604
PpsI GAGTC 4 cut(s) 22, 206, 506, 604
PpuMI RGGWCCY 1 cut(s) 124
PscI ACATGT 1 cut(s) 1043
PshBI ATTAAT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 124
Psp6I CCWGG 2 cut(s) 773, 787
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 773, 787
PspN4I GGNNCC 3 cut(s) 125, 126, 604
PspPI GGNCC 2 cut(s) 124, 752
PspPPI RGGWCCY 1 cut(s) 124
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 4 cut(s) 393, 842, 887, 1000
RsaNI GTAC 4 cut(s) 392, 841, 886, 999
SalI GTCGAC 1 cut(s) 26
SaqAI TTAA 4 cut(s) 444, 801, 834, 1158
SatI GCNGC 4 cut(s) 86, 463, 991, 1016
Sau3AI GATC 1 cut(s) 195
Sau96I GGNCC 2 cut(s) 124, 752
SchI GAGTC 4 cut(s) 23, 206, 507, 605
ScrFI CCNGG 5 cut(s) 656, 775, 789, 891, 892
SduI GDGCHC 3 cut(s) 769, 781, 796
SfaNI GCATC 1 cut(s) 649
SinI GGWCC 1 cut(s) 124
SmaI CCCGGG 1 cut(s) 892
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
SpeI ACTAGT 1 cut(s) 538
SphI GCATGC 1 cut(s) 1100
Sse9I AATT 9 cut(s) 353, 396, 490, 518, 527, 695, 732, 1146, 1159
SseBI AGGCCT 1 cut(s) 1121
SsiI CCGC 3 cut(s) 465, 990, 993
SspMI CTAG 7 cut(s) 453, 539, 569, 650, 749, 1004, 1172
StuI AGGCCT 1 cut(s) 1121
StyD4I CCNGG 5 cut(s) 654, 773, 787, 889, 890
StyI CCWWGG 1 cut(s) 1116
TaaI ACNGT 4 cut(s) 92, 259, 279, 334
TaiI ACGT 2 cut(s) 958, 975
TaqI TCGA 7 cut(s) 27, 222, 594, 805, 940, 977, 1144
TasI AATT 9 cut(s) 353, 396, 490, 518, 527, 695, 732, 1146, 1159
TatI WGTACW 1 cut(s) 840
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 3 cut(s) 32, 155, 164
Tru1I TTAA 4 cut(s) 444, 801, 834, 1158
Tru9I TTAA 4 cut(s) 444, 801, 834, 1158
TscAI CASTG 2 cut(s) 187, 262
TseFI GTSAC 2 cut(s) 499, 688
TseI GCWGC 3 cut(s) 85, 462, 1015
Tsp45I GTSAC 2 cut(s) 499, 688
TspDTI ATGAA 5 cut(s) 156, 334, 346, 688, 836
TspMI CCCGGG 1 cut(s) 890
TspRI CASTG 2 cut(s) 187, 262
Van91I CCANNNNNTGG 1 cut(s) 281
VneI GTGCAC 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 124
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 2 cut(s) 490, 695
XceI RCATGY 4 cut(s) 718, 987, 1047, 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmaI CCCGGG 1 cut(s) 890
XmiI GTMKAC 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 7 cut(s) 453, 539, 569, 650, 749, 1004, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.