Rh4DG330300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016027
Physical Location & Seq
Reverse (-)
55320946 .. 55321991
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG330300.1

Sequence Viewer

Length: 708 bp
ATGATGAAAACCACACTACTACTACTTCTCCTTCTTGTATGTTCTTCATCTGTTTCATCATCAGATTCATCTCTAATATCCAATGCTGTTTGTGGGATCTTCACTTTCGGAGACTCCATCTTCGACGCTGGAAACAATCACTTCAACAAGAACTGCACTGTTCAAGCAGATTTCCCACCGTATGGCTCAAGCTTCTTCCACCACCCAACTGGCAGGTTCACTAATGGCAGAACTGTTGCTGATTTCATCTCACAATTCATCGGCATTCCTCTCCAAAAACCATATTATGAAGTACAAATTGAAGCTATGACTGGAAGCCGGAAAGGTTATCCTTCCAATGGCCTTAACTTTGCTAGTGCCGGCAGTGGTGTTTTGCAAGGAACAAATAAAAATTTGGGAGTCACCTCATTCCAAGAACAATTACAACAATTCCAGACACTAGTGGAGCAAAACCACATTGACAAAACTCTAGTTCAGAAATCCTTCTTCTTCTTCGAGTCTGGTTCCAACGACATCTTCAACTACTTTCTTCCATTAATTTGCTCCCCCAACACTAGACCCGGATGCCTATGTCCAAGCCATGCTAACGCAAGTCACAAAATTCATAGACCAAATCTACATGCTTGGCACTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGTTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

235

Amino Acids

25.92

Weight (kDa)

8.34

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 184 3.8e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 182
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 2 cut(s) 391, 600
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 182, 338
AgsI TTSAA 4 cut(s) 145, 164, 302, 520
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 2 cut(s) 678, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 2 cut(s) 192, 305
AluI AGCT 2 cut(s) 192, 305
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 697
AlwI GGATC 1 cut(s) 104
AoxI GGCC 2 cut(s) 340, 657
ApaLI GTGCAC 1 cut(s) 697
ApoI RAATTY 2 cut(s) 391, 600
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AseI ATTAAT 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 657
AsuC2I CCSGG 1 cut(s) 561
AsuHPI GGTGA 1 cut(s) 394
BaeGI GKGCMC 2 cut(s) 686, 701
Bbv12I GWGCWC 1 cut(s) 701
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 680, 694
BcnI CCSGG 1 cut(s) 561
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 5 cut(s) 354, 440, 470, 555, 654
BfuAI ACCTGC 1 cut(s) 204
Bme1390I CCNGG 3 cut(s) 561, 680, 694
BmgT120I GGNCC 1 cut(s) 657
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 3 cut(s) 561, 680, 694
BmsI GCATC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 561
BsaJI CCNNGG 1 cut(s) 679
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 338
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 214, 316
BseBI CCWGG 2 cut(s) 680, 694
BseDI CCNNGG 1 cut(s) 679
BseGI GGATG 1 cut(s) 569
BseLI CCNNNNNNNGG 2 cut(s) 182, 338
BseNI ACTGG 2 cut(s) 214, 316
BseSI GKGCMC 2 cut(s) 686, 701
BsgI GTGCAG 1 cut(s) 139
BshFI GGCC 2 cut(s) 342, 659
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 3 cut(s) 319, 360, 561
BslI CCNNNNNNNGG 2 cut(s) 182, 338
BsmAI GTCTC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 2 cut(s) 342, 659
Bsp1286I GDGCHC 2 cut(s) 686, 701
Bsp143I GATC 1 cut(s) 96
BspANI GGCC 2 cut(s) 342, 659
BspLI GGNNCC 1 cut(s) 505
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 2 cut(s) 214, 316
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 679
BssMI GATC 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 680, 694
Bst4CI ACNGT 3 cut(s) 160, 180, 235
BstC8I GCNNGC 1 cut(s) 361
BstF5I GGATG 1 cut(s) 569
BstKTI GATC 1 cut(s) 99
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 96
BstNI CCWGG 2 cut(s) 680, 694
BstNSI RCATGY 1 cut(s) 623
BstSCI CCNGG 3 cut(s) 559, 678, 692
BstSLI GKGCMC 2 cut(s) 686, 701
BstX2I RGATCY 1 cut(s) 96
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 1 cut(s) 96
BsuRI GGCC 2 cut(s) 342, 659
BtsCI GGATG 1 cut(s) 569
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 2 cut(s) 156, 370
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 657
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 581, 620
CviJI RGCY 9 cut(s) 186, 192, 305, 318, 342, 579, 659, 667, 678
CviKI_1 RGCY 9 cut(s) 186, 192, 305, 318, 342, 579, 659, 667, 678
CviQI GTAC 1 cut(s) 293
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 657
EcoRII CCWGG 2 cut(s) 678, 692
FaeI CATG 2 cut(s) 584, 623
FaiI YATR 9 cut(s) 40, 183, 283, 288, 308, 571, 582, 606, 621
FatI CATG 2 cut(s) 580, 619
FokI GGATG 1 cut(s) 576
FspBI CTAG 5 cut(s) 354, 440, 470, 555, 654
HaeIII GGCC 2 cut(s) 342, 659
HapII CCGG 3 cut(s) 319, 360, 561
HgaI GACGC 1 cut(s) 134
Hin1II CATG 2 cut(s) 584, 623
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 4 cut(s) 65, 113, 399, 497
HpaII CCGG 3 cut(s) 319, 360, 561
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 2 cut(s) 219, 699
Hpy188I TCNGA 3 cut(s) 64, 110, 477
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 2 cut(s) 219, 699
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 41, 342, 493
HpyCH4III ACNGT 3 cut(s) 160, 180, 235
HpyCH4V TGCA 3 cut(s) 156, 376, 699
Hsp92II CATG 2 cut(s) 584, 623
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 445, 548
LweI GCATC 1 cut(s) 554
MaeI CTAG 5 cut(s) 354, 440, 470, 555, 654
MaeIII GTNAC 2 cut(s) 400, 593
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 9 cut(s) 36, 91, 112, 187, 478, 481, 484, 508, 521
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 2 cut(s) 686, 701
MluCI AATT 8 cut(s) 254, 297, 391, 419, 428, 537, 600, 637
MlyI GAGTC 3 cut(s) 107, 408, 506
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 2 cut(s) 279, 415
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 3 cut(s) 345, 536, 706
MspI CCGG 3 cut(s) 319, 360, 561
MspR9I CCNGG 3 cut(s) 561, 680, 694
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 2 cut(s) 680, 694
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 561
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 2 cut(s) 584, 623
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 400, 593
NspI RCATGY 1 cut(s) 623
PctI GAATGC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 182
PleI GAGTC 3 cut(s) 107, 407, 505
PpsI GAGTC 3 cut(s) 107, 407, 505
PshBI ATTAAT 1 cut(s) 536
Psp6I CCWGG 2 cut(s) 678, 692
PspGI CCWGG 2 cut(s) 678, 692
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 1 cut(s) 657
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 345, 536, 706
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 657
SchI GAGTC 3 cut(s) 107, 408, 506
ScrFI CCNGG 3 cut(s) 561, 680, 694
SduI GDGCHC 2 cut(s) 686, 701
SetI ASST 6 cut(s) 194, 218, 307, 328, 407, 698
SfaNI GCATC 1 cut(s) 554
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 8 cut(s) 254, 297, 391, 419, 428, 537, 600, 637
SspMI CTAG 5 cut(s) 354, 440, 470, 555, 654
StyD4I CCNGG 3 cut(s) 559, 678, 692
TaaI ACNGT 3 cut(s) 160, 180, 235
TaqI TCGA 2 cut(s) 123, 495
TasI AATT 8 cut(s) 254, 297, 391, 419, 428, 537, 600, 637
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 3 cut(s) 345, 536, 706
Tru9I TTAA 3 cut(s) 345, 536, 706
TscAI CASTG 2 cut(s) 163, 370
TseFI GTSAC 2 cut(s) 400, 593
Tsp45I GTSAC 2 cut(s) 400, 593
TspDTI ATGAA 8 cut(s) 20, 36, 45, 57, 235, 247, 303, 593
TspRI CASTG 2 cut(s) 163, 370
Van91I CCANNNNNTGG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 697
VspI ATTAAT 1 cut(s) 536
XapI RAATTY 2 cut(s) 391, 600
XceI RCATGY 1 cut(s) 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 5 cut(s) 354, 440, 470, 555, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.