Rw4G028220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016027
Physical Location & Seq
Reverse (-)
54874678 .. 54877466
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028220.1

Sequence Viewer

Length: 822 bp
ATGGCTGCTACTCCTTGGCCACCAACTATGACTGGAAGCCGGAAAGGTTATCCTTCCAATGGCCTTAACTTTGCTAGTGCCGGCAGTGGTGTTTTGCAAGGAACAAATAAAAATTTGGGAGTCACCTCACTCCAAGAACAATTACAACAATTCCAGACACTAGTGGAGCAAAACCACATTGACAAAACTCTAGTTCAGAAATCCTTCTTCTTCTTCGAGTCTGGTTCCAACGACATCTTCAACTACTTTCTTCCATTTGCTCCCCCAACACTAGACCAGGATGCCTATGTCCAAGCCATGCTAACCCAAGTCACAAAATTCATAGACCAAATCTACATGCTTGGCACTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGTTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAATCGATGTTTCGGAAAGATGAACTTGATGGTTAAGAAGTACAACAAGGGACTGGAATGTTTGGTCAATGATATGCCAATCAAGTACCCCGGGGTGGTGGGTGTTTATGGAGAAACCTATGACATTGTTCAGCGATTTCGAGCCATTCCTACACGTTACCATTTTTCGGATGTATCGAGAGCATGTTGCGGCGGAGGTACTCTAGGAGGATTGCTGCAATGTGGGAAGGAGGGATACAAGACATGTGCAAACCCTAATGAGTTCTTATTCTGGGATTACTTCCATCCATCAGAGCATGCTTACAGGCTCATATCCAAGGCCTTGTGGGGTGGAAAGAAATATCGAATTAGACCACTTAATTTGAAGGCTCTAGCCAACATCACCCTCACTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

273

Amino Acids

30.32

Weight (kDa)

9.34

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 15 - 249 1e-20 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 612, 615
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 2 cut(s) 112, 317
AfaI GTAC 3 cut(s) 464, 509, 622
AfiI CCNNNNNNNGG 3 cut(s) 59, 517, 589
AflIII ACRYGT 2 cut(s) 575, 665
AgsI TTSAA 2 cut(s) 241, 787
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 3 cut(s) 276, 395, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
Alw21I GWGCWC 1 cut(s) 418
Alw44I GTGCAC 1 cut(s) 414
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 4 cut(s) 17, 61, 374, 741
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 5, 637
ApoI RAATTY 2 cut(s) 112, 317
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 2 cut(s) 513, 514
AsuHPI GGTGA 2 cut(s) 115, 796
AvaI CYCGRG 1 cut(s) 512
BaeGI GKGCMC 2 cut(s) 403, 418
BalI TGGCCA 1 cut(s) 19
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 1 cut(s) 624
BccI CCATC 3 cut(s) 445, 714, 718
BciT130I CCWGG 3 cut(s) 278, 397, 411
BciVI GTATCC 1 cut(s) 650
BcnI CCSGG 2 cut(s) 513, 514
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 7 cut(s) 75, 161, 191, 272, 371, 626, 794
BfuI GTATCC 1 cut(s) 650
BisI GCNGC 3 cut(s) 6, 613, 638
BlsI GCNGC 3 cut(s) 7, 614, 639
Bme1390I CCNGG 5 cut(s) 278, 397, 411, 513, 514
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 226
BmrFI CCNGG 5 cut(s) 278, 397, 411, 513, 514
BmsI GCATC 1 cut(s) 271
BpuMI CCSGG 2 cut(s) 513, 514
Bsa29I ATCGAT 1 cut(s) 427
BsaJI CCNNGG 6 cut(s) 14, 396, 511, 512, 513, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 517, 589
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 3 cut(s) 37, 480, 817
Bse3DI GCAATG 1 cut(s) 647
BseBI CCWGG 3 cut(s) 278, 397, 411
BseCI ATCGAT 1 cut(s) 427
BseDI CCNNGG 6 cut(s) 14, 396, 511, 512, 513, 738
BseGI GGATG 3 cut(s) 286, 598, 706
BseLI CCNNNNNNNGG 3 cut(s) 59, 517, 589
BseMI GCAATG 1 cut(s) 647
BseNI ACTGG 3 cut(s) 37, 480, 817
BseSI GKGCMC 2 cut(s) 403, 418
BseXI GCAGC 1 cut(s) 624
BshFI GGCC 4 cut(s) 19, 63, 376, 743
BshVI ATCGAT 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 40, 81, 513
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 3 cut(s) 59, 517, 589
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 4 cut(s) 19, 63, 376, 743
BsoBI CYCGRG 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 403, 418
BspACI CCGC 2 cut(s) 612, 615
BspANI GGCC 4 cut(s) 19, 63, 376, 743
BspDI ATCGAT 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 3 cut(s) 37, 480, 817
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 6 cut(s) 14, 396, 511, 512, 513, 738
BssT1I CCWWGG 2 cut(s) 14, 738
Bst2UI CCWGG 3 cut(s) 278, 397, 411
BstC8I GCNNGC 2 cut(s) 82, 720
BstF5I GGATG 3 cut(s) 286, 598, 706
BstNI CCWGG 3 cut(s) 278, 397, 411
BstNSI RCATGY 4 cut(s) 340, 609, 669, 722
BstSCI CCNGG 5 cut(s) 276, 395, 409, 511, 512
BstSLI GKGCMC 2 cut(s) 403, 418
BstV1I GCAGC 1 cut(s) 624
Bsu15I ATCGAT 1 cut(s) 427
BsuI GTATCC 1 cut(s) 650
BsuRI GGCC 4 cut(s) 19, 63, 376, 743
BsuTUI ATCGAT 1 cut(s) 427
BtsCI GGATG 3 cut(s) 286, 598, 706
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 91, 810
Cac8I GCNNGC 2 cut(s) 82, 720
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 374
Cfr9I CCCGGG 1 cut(s) 512
ClaI ATCGAT 1 cut(s) 427
Csp6I GTAC 3 cut(s) 463, 508, 621
CspCI CAANNNNNGTGG 2 cut(s) 765, 800
CviAII CATG 5 cut(s) 298, 337, 606, 666, 719
CviQI GTAC 3 cut(s) 463, 508, 621
EaeI YGGCCR 1 cut(s) 17
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 2 cut(s) 14, 738
Eco147I AGGCCT 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 374
EcoRII CCWGG 3 cut(s) 276, 395, 409
EcoT14I CCWWGG 2 cut(s) 14, 738
ErhI CCWWGG 2 cut(s) 14, 738
FaeI CATG 5 cut(s) 301, 340, 609, 669, 722
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 486
FatI CATG 5 cut(s) 297, 336, 605, 665, 718
Fnu4HI GCNGC 3 cut(s) 6, 613, 638
FokI GGATG 3 cut(s) 293, 605, 693
Fsp4HI GCNGC 3 cut(s) 6, 613, 638
FspBI CTAG 7 cut(s) 75, 161, 191, 272, 371, 626, 794
GluI GCNGC 3 cut(s) 6, 613, 638
HaeIII GGCC 4 cut(s) 19, 63, 376, 743
HapII CCGG 3 cut(s) 40, 81, 513
Hin1II CATG 5 cut(s) 301, 340, 609, 669, 722
HinfI GANTC 2 cut(s) 120, 218
HpaII CCGG 3 cut(s) 40, 81, 513
HphI GGTGA 2 cut(s) 115, 796
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 4 cut(s) 198, 437, 592, 715
Hpy188III TCNNGA 2 cut(s) 154, 600
Hpy8I GTNNAC 1 cut(s) 416
HpyAV CCTTC 4 cut(s) 63, 214, 643, 781
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 4 cut(s) 97, 416, 640, 671
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 5 cut(s) 301, 340, 609, 669, 722
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 166, 265
Lsp1109I GCAGC 1 cut(s) 624
LweI GCATC 1 cut(s) 271
MaeI CTAG 7 cut(s) 75, 161, 191, 272, 371, 626, 794
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 3 cut(s) 121, 310, 578
MboII GAAGA 5 cut(s) 199, 202, 205, 229, 242
MhlI GDGCHC 2 cut(s) 403, 418
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 7 cut(s) 112, 140, 149, 317, 354, 768, 781
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 2 cut(s) 129, 227
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 5 cut(s) 136, 611, 623, 646, 818
Mox20I TGGCCA 1 cut(s) 19
MroNI GCCGGC 1 cut(s) 80
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 19
MseI TTAA 4 cut(s) 66, 423, 456, 780
Msp20I TGGCCA 1 cut(s) 19
MspI CCGG 3 cut(s) 40, 81, 513
MspR9I CCNGG 5 cut(s) 278, 397, 411, 513, 514
MvaI CCWGG 3 cut(s) 278, 397, 411
NaeI GCCGGC 1 cut(s) 82
NciI CCSGG 2 cut(s) 513, 514
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 5 cut(s) 301, 340, 609, 669, 722
NlaIV GGNNCC 1 cut(s) 226
NmuCI GTSAC 2 cut(s) 121, 310
NspI RCATGY 4 cut(s) 340, 609, 669, 722
PaeI GCATGC 1 cut(s) 722
PceI AGGCCT 1 cut(s) 743
PciI ACATGT 1 cut(s) 665
PcsI WCGNNNNNNNCGW 1 cut(s) 596
PdiI GCCGGC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 203
PkrI GCNGC 3 cut(s) 7, 614, 639
PleI GAGTC 2 cut(s) 128, 226
PpsI GAGTC 2 cut(s) 128, 226
PscI ACATGT 1 cut(s) 665
PshBI ATTAAT 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 276, 395, 409
PspGI CCWGG 3 cut(s) 276, 395, 409
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 1 cut(s) 374
RsaI GTAC 3 cut(s) 464, 509, 622
RsaNI GTAC 3 cut(s) 463, 508, 621
SaqAI TTAA 4 cut(s) 66, 423, 456, 780
SatI GCNGC 3 cut(s) 6, 613, 638
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 2 cut(s) 129, 227
ScrFI CCNGG 5 cut(s) 278, 397, 411, 513, 514
SduI GDGCHC 2 cut(s) 403, 418
SetI ASST 6 cut(s) 49, 128, 415, 542, 580, 622
SfaNI GCATC 1 cut(s) 271
SmaI CCCGGG 1 cut(s) 514
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 722
Sse9I AATT 7 cut(s) 112, 140, 149, 317, 354, 768, 781
SseBI AGGCCT 1 cut(s) 743
SsiI CCGC 2 cut(s) 612, 615
SspMI CTAG 7 cut(s) 75, 161, 191, 272, 371, 626, 794
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 5 cut(s) 276, 395, 409, 511, 512
StyI CCWWGG 2 cut(s) 14, 738
TaiI ACGT 1 cut(s) 580
TaqI TCGA 5 cut(s) 216, 427, 562, 599, 766
TasI AATT 7 cut(s) 112, 140, 149, 317, 354, 768, 781
TatI WGTACW 1 cut(s) 462
TauI GCSGC 1 cut(s) 615
Tru1I TTAA 4 cut(s) 66, 423, 456, 780
Tru9I TTAA 4 cut(s) 66, 423, 456, 780
TscAI CASTG 2 cut(s) 91, 817
TseFI GTSAC 2 cut(s) 121, 310
TseI GCWGC 2 cut(s) 5, 637
Tsp45I GTSAC 2 cut(s) 121, 310
TspDTI ATGAA 2 cut(s) 310, 458
TspMI CCCGGG 1 cut(s) 512
TspRI CASTG 2 cut(s) 91, 817
VneI GTGCAC 1 cut(s) 414
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 2 cut(s) 112, 317
XceI RCATGY 4 cut(s) 340, 609, 669, 722
XmaI CCCGGG 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 7 cut(s) 75, 161, 191, 272, 371, 626, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.