Rroxscaffold_5G00375190

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016027
Physical Location & Seq
Reverse (-)
56036218 .. 56042445
6228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375190.1

Sequence Viewer

Length: 1101 bp
ATGATGAAAACCACACTACTTCTACATCTCCTTCTTGTATGTTCTTCATCTGTTTCATCATCAGATTCATCTCTAATATCCAATGCTGTTTGTGGGATCTTCACTTTCGGAGACTCCATCTTCGACGCTGGAAACAATCACTTCAACAAGAACTGCACTGTTCAAGCAGATTTCCCACCGTATGGCTCAAGCTTCTTCCACTACCCGACTGGCAGGTTCACTAATGGCAGAACCGTTGCTGATTTCATCTCACAATTCATCGGCATTCCTCTCCAAAAACCATATTATGAGGTACAAATTGAAGCTATGACTGGAAGCCGGAAAGGTTGTCCTTCCTATGGCATTAACTTTGCTAGTGCCGGCAGTGGTGTTTTGAAAGGAACAAATAAAAATTTGGGAGTCACCTCACTCCAAGAACAATTACTACAATTCCAGACACTAGTGGAGCAAAACCACAATGACAAAACTCTAGTTCAAAAATCCTTCTTCTTCTTCGAGTCTGGTTCCAACGACATCTTCAACTACTTTCTTCCATTTCCTCCCCCAACACTAGACCCGGATGCCTATGTCCAAGTCATGCTAACCCAAGTCACAAAATTCATAGACCAAATCTACATGCATGGCACTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGTTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAATCGATGTTTCGGAAAGATGAACTTGATGGTTAAGAAGTACAACAAGGGACTGGAATGTTTGGTCAATGATATGCCTATCAAGTACCCCGGGGTGGTGGGTGTTTATGGAGAAACCTATGACATTGTTCAGCGATTTCGAGCCATTCCTACACGTTACCATTTTTCGGACGTATCGAGCGCATGTTGCGGCGGAGGTACTCTAGGAGGATTGCTGCAATGTGGGAAGGAGGGATACAAGACATGTGCAGACCGTAATGAATTCTTATTCTGGGATTACTTCCATCCATCAGAGCATGCTTACAGGCTCATATCCAAGGCCTTGTGGGGTGGAAAGAAATATCGAATTAGACCACTTAATTTGAAGGCTCTAGCCAACATCACCCTCACTCGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.69

Weight (kDa)

9.01

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 342 6.3e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 182
AciI CCGC 2 cut(s) 891, 894
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 3 cut(s) 391, 596, 962
AfaI GTAC 4 cut(s) 294, 743, 788, 901
AfiI CCNNNNNNNGG 4 cut(s) 182, 338, 796, 868
AflIII ACRYGT 2 cut(s) 854, 944
AgsI TTSAA 7 cut(s) 145, 164, 302, 376, 476, 520, 1066
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 2 cut(s) 674, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 2 cut(s) 192, 305
AluI AGCT 2 cut(s) 192, 305
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 2 cut(s) 653, 1020
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 1 cut(s) 916
ApoI RAATTY 3 cut(s) 391, 596, 962
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AseI ATTAAT 1 cut(s) 702
AspLEI GCGC 1 cut(s) 884
AspS9I GGNCC 1 cut(s) 653
AsuC2I CCSGG 3 cut(s) 557, 792, 793
AsuHPI GGTGA 2 cut(s) 394, 1075
AvaI CYCGRG 1 cut(s) 791
BaeGI GKGCMC 2 cut(s) 682, 697
BarI GAAGNNNNNNTAC 2 cut(s) 920, 952
BauI CACGAG 1 cut(s) 1092
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 1 cut(s) 903
BccI CCATC 4 cut(s) 125, 724, 993, 997
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 2 cut(s) 676, 690
BciVI GTATCC 1 cut(s) 929
BcnI CCSGG 3 cut(s) 557, 792, 793
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 7 cut(s) 354, 440, 470, 551, 650, 905, 1073
BfuAI ACCTGC 1 cut(s) 204
BfuI GTATCC 1 cut(s) 929
BisI GCNGC 2 cut(s) 892, 917
BlsI GCNGC 2 cut(s) 893, 918
Bme1390I CCNGG 5 cut(s) 557, 676, 690, 792, 793
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 5 cut(s) 557, 676, 690, 792, 793
BmsI GCATC 1 cut(s) 550
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 3 cut(s) 557, 792, 793
Bsa29I ATCGAT 1 cut(s) 706
BsaJI CCNNGG 5 cut(s) 675, 790, 791, 792, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 338, 796, 868
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 214, 316, 759
Bse3DI GCAATG 1 cut(s) 926
BseBI CCWGG 2 cut(s) 676, 690
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 5 cut(s) 675, 790, 791, 792, 1017
BseGI GGATG 2 cut(s) 565, 985
BseLI CCNNNNNNNGG 4 cut(s) 182, 338, 796, 868
BseMI GCAATG 1 cut(s) 926
BseNI ACTGG 3 cut(s) 214, 316, 759
BseSI GKGCMC 2 cut(s) 682, 697
BseXI GCAGC 1 cut(s) 903
BsgI GTGCAG 2 cut(s) 139, 969
BshFI GGCC 2 cut(s) 655, 1022
BshVI ATCGAT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 4 cut(s) 319, 360, 557, 792
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 4 cut(s) 182, 338, 796, 868
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 2 cut(s) 655, 1022
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 2 cut(s) 682, 697
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 2 cut(s) 891, 894
BspANI GGCC 2 cut(s) 655, 1022
BspDI ATCGAT 1 cut(s) 706
BspLI GGNNCC 1 cut(s) 505
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 926
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 214, 316, 759
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 5 cut(s) 675, 790, 791, 792, 1017
BssMI GATC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 1092
BssT1I CCWWGG 1 cut(s) 1017
Bst2BI CACGAG 1 cut(s) 1092
Bst2UI CCWGG 2 cut(s) 676, 690
Bst4CI ACNGT 4 cut(s) 160, 180, 235, 956
BstC8I GCNNGC 2 cut(s) 361, 999
BstF5I GGATG 2 cut(s) 565, 985
BstHHI GCGC 1 cut(s) 884
BstKTI GATC 1 cut(s) 99
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 888
BstNI CCWGG 2 cut(s) 676, 690
BstNSI RCATGY 4 cut(s) 619, 888, 948, 1001
BstSCI CCNGG 5 cut(s) 555, 674, 688, 790, 791
BstSLI GKGCMC 2 cut(s) 682, 697
BstV1I GCAGC 1 cut(s) 903
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 706
BsuI GTATCC 1 cut(s) 929
BsuRI GGCC 2 cut(s) 655, 1022
BsuTUI ATCGAT 1 cut(s) 706
BtsCI GGATG 2 cut(s) 565, 985
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 2 cut(s) 156, 370
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 361, 999
CfoI GCGC 1 cut(s) 884
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 653
Cfr9I CCCGGG 1 cut(s) 791
ClaI ATCGAT 1 cut(s) 706
CseI GACGC 1 cut(s) 134
Csp6I GTAC 4 cut(s) 293, 742, 787, 900
CspCI CAANNNNNGTGG 2 cut(s) 1044, 1079
CviAII CATG 6 cut(s) 577, 616, 620, 885, 945, 998
CviQI GTAC 4 cut(s) 293, 742, 787, 900
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 909
Eco130I CCWWGG 1 cut(s) 1017
Eco147I AGGCCT 1 cut(s) 1022
Eco88I CYCGRG 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 653
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 2 cut(s) 674, 688
EcoT14I CCWWGG 1 cut(s) 1017
EcoT22I ATGCAT 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 1017
FaeI CATG 6 cut(s) 580, 619, 623, 888, 948, 1001
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 765
FatI CATG 6 cut(s) 576, 615, 619, 884, 944, 997
Fnu4HI GCNGC 2 cut(s) 892, 917
FokI GGATG 2 cut(s) 572, 972
Fsp4HI GCNGC 2 cut(s) 892, 917
FspBI CTAG 7 cut(s) 354, 440, 470, 551, 650, 905, 1073
GlaI GCGC 1 cut(s) 883
GluI GCNGC 2 cut(s) 892, 917
HaeIII GGCC 2 cut(s) 655, 1022
HapII CCGG 4 cut(s) 319, 360, 557, 792
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 884
Hin1II CATG 6 cut(s) 580, 619, 623, 888, 948, 1001
Hin6I GCGC 1 cut(s) 882
HinP1I GCGC 1 cut(s) 882
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 4 cut(s) 65, 113, 399, 497
HpaII CCGG 4 cut(s) 319, 360, 557, 792
HphI GGTGA 2 cut(s) 394, 1075
Hpy166II GTNNAC 2 cut(s) 219, 695
Hpy188I TCNGA 5 cut(s) 64, 110, 716, 871, 994
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 2 cut(s) 219, 695
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 41, 342, 493, 922, 1060
HpyCH4III ACNGT 4 cut(s) 160, 180, 235, 956
HpyCH4IV ACGT 2 cut(s) 856, 873
HpyCH4V TGCA 5 cut(s) 156, 619, 695, 919, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 888
HpySE526I ACGT 2 cut(s) 856, 873
Hsp92II CATG 6 cut(s) 580, 619, 623, 888, 948, 1001
HspAI GCGC 1 cut(s) 882
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 903
LweI GCATC 1 cut(s) 550
MaeI CTAG 7 cut(s) 354, 440, 470, 551, 650, 905, 1073
MaeII ACGT 2 cut(s) 856, 873
MaeIII GTNAC 3 cut(s) 400, 589, 857
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 9 cut(s) 36, 91, 112, 187, 478, 481, 484, 508, 521
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 2 cut(s) 682, 697
MlyI GAGTC 3 cut(s) 107, 408, 506
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 8 cut(s) 279, 283, 415, 549, 890, 902, 925, 1097
Mph1103I ATGCAT 1 cut(s) 621
MroNI GCCGGC 1 cut(s) 359
MseI TTAA 4 cut(s) 345, 702, 735, 1059
MspI CCGG 4 cut(s) 319, 360, 557, 792
MspR9I CCNGG 5 cut(s) 557, 676, 690, 792, 793
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 2 cut(s) 676, 690
MwoI GCNNNNNNNGC 1 cut(s) 888
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 3 cut(s) 557, 792, 793
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 6 cut(s) 580, 619, 623, 888, 948, 1001
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 400, 589
NsiI ATGCAT 1 cut(s) 621
NspI RCATGY 4 cut(s) 619, 888, 948, 1001
PaeI GCATGC 1 cut(s) 1001
PceI AGGCCT 1 cut(s) 1022
PciI ACATGT 1 cut(s) 944
PcsI WCGNNNNNNNCGW 1 cut(s) 875
PctI GAATGC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 182
PkrI GCNGC 2 cut(s) 893, 918
PleI GAGTC 3 cut(s) 107, 407, 505
PpsI GAGTC 3 cut(s) 107, 407, 505
PscI ACATGT 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 674, 688
PspGI CCWGG 2 cut(s) 674, 688
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 1 cut(s) 653
PsrI GAACNNNNNNTAC 2 cut(s) 408, 440
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 4 cut(s) 294, 743, 788, 901
RsaNI GTAC 4 cut(s) 293, 742, 787, 900
SaqAI TTAA 4 cut(s) 345, 702, 735, 1059
SatI GCNGC 2 cut(s) 892, 917
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 3 cut(s) 107, 408, 506
ScrFI CCNGG 5 cut(s) 557, 676, 690, 792, 793
SduI GDGCHC 2 cut(s) 682, 697
SfaNI GCATC 1 cut(s) 550
SmaI CCCGGG 1 cut(s) 793
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 439
SphI GCATGC 1 cut(s) 1001
SseBI AGGCCT 1 cut(s) 1022
SsiI CCGC 2 cut(s) 891, 894
SspMI CTAG 7 cut(s) 354, 440, 470, 551, 650, 905, 1073
StuI AGGCCT 1 cut(s) 1022
StyD4I CCNGG 5 cut(s) 555, 674, 688, 790, 791
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 4 cut(s) 160, 180, 235, 956
TaiI ACGT 2 cut(s) 859, 876
TaqI TCGA 6 cut(s) 123, 495, 706, 841, 878, 1045
TatI WGTACW 1 cut(s) 741
TauI GCSGC 1 cut(s) 894
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 345, 702, 735, 1059
Tru9I TTAA 4 cut(s) 345, 702, 735, 1059
TscAI CASTG 2 cut(s) 163, 370
TseFI GTSAC 2 cut(s) 400, 589
TseI GCWGC 1 cut(s) 916
Tsp45I GTSAC 2 cut(s) 400, 589
TspDTI ATGAA 9 cut(s) 20, 36, 45, 57, 235, 247, 589, 737, 975
TspMI CCCGGG 1 cut(s) 791
TspRI CASTG 2 cut(s) 163, 370
Van91I CCANNNNNTGG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 693
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 3 cut(s) 391, 596, 962
XceI RCATGY 4 cut(s) 619, 888, 948, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 791
XspI CTAG 7 cut(s) 354, 440, 470, 551, 650, 905, 1073
Zsp2I ATGCAT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.