RchiOBHm_Chr4g0433751

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016027
Physical Location & Seq
Reverse (-)
57758655 .. 57759369
715 bp
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UTR
Exon/CDS
Intron
PRQ40222

Sequence Viewer

Length: 432 bp
ATGCTAACGCAAGTCACAAAATTCATAGACCAAATCTACATGCTTGGCACTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGTTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAATCGATGTTTTGGAAAGATGAACTTGATGGTTAAGAAGTACAACAAGGGACGTACTGGAATGTTTGATTTTTCGGACGTATCGAGCGCATGTTGCGGCGGAGGTACTATAGGAGGATTGCTGCAATGTGGGAAGGAGGGATACAAGACATGTGCAAACCCTAATGAGTTCTTATTCTGGGATTACTTCCATCCATCAGAGCATGCTTACAGGCTCATATCCAAGGCCTTGTGGGGTGGAAAGAAATATCGAATTAGACCACTTAATTTGAAGGCTCTAGCCAACATCACCCTCACTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

143

Amino Acids

15.79

Weight (kDa)

9.73

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 3 - 119 2e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 222, 225
AcsI RAATTY 1 cut(s) 20
AfaI GTAC 3 cut(s) 167, 181, 232
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 1 cut(s) 397
AjnI CCWGG 2 cut(s) 98, 112
Alw21I GWGCWC 1 cut(s) 121
Alw44I GTGCAC 1 cut(s) 117
AoxI GGCC 2 cut(s) 77, 351
ApaLI GTGCAC 1 cut(s) 117
ApeKI GCWGC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 20
AseI ATTAAT 1 cut(s) 126
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 1 cut(s) 406
BaeGI GKGCMC 2 cut(s) 106, 121
BarI GAAGNNNNNNTAC 2 cut(s) 251, 283
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 234
BccI CCATC 3 cut(s) 148, 324, 328
BciT130I CCWGG 2 cut(s) 100, 114
BciVI GTATCC 1 cut(s) 260
BfaI CTAG 2 cut(s) 74, 404
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 2 cut(s) 223, 248
BlsI GCNGC 2 cut(s) 224, 249
Bme1390I CCNGG 2 cut(s) 100, 114
BmgT120I GGNCC 1 cut(s) 77
BmrFI CCNGG 2 cut(s) 100, 114
Bsa29I ATCGAT 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 99, 348
Bse1I ACTGG 2 cut(s) 187, 427
Bse3DI GCAATG 1 cut(s) 257
BseBI CCWGG 2 cut(s) 100, 114
BseCI ATCGAT 1 cut(s) 130
BseDI CCNNGG 2 cut(s) 99, 348
BseGI GGATG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 257
BseNI ACTGG 2 cut(s) 187, 427
BseSI GKGCMC 2 cut(s) 106, 121
BseXI GCAGC 1 cut(s) 234
BshFI GGCC 2 cut(s) 79, 353
BshVI ATCGAT 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 121
BslFI GGGAC 1 cut(s) 189
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 2 cut(s) 79, 353
Bsp1286I GDGCHC 2 cut(s) 106, 121
BspACI CCGC 2 cut(s) 222, 225
BspANI GGCC 2 cut(s) 79, 353
BspDI ATCGAT 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 257
BsrI ACTGG 2 cut(s) 187, 427
BssECI CCNNGG 2 cut(s) 99, 348
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 2 cut(s) 100, 114
BstC8I GCNNGC 1 cut(s) 330
BstF5I GGATG 1 cut(s) 316
BstHHI GCGC 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNI CCWGG 2 cut(s) 100, 114
BstNSI RCATGY 4 cut(s) 43, 219, 279, 332
BstSCI CCNGG 2 cut(s) 98, 112
BstSFI CTRYAG 1 cut(s) 234
BstSLI GKGCMC 2 cut(s) 106, 121
BstV1I GCAGC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 130
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 2 cut(s) 79, 353
BsuTUI ATCGAT 1 cut(s) 130
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 130
Csp6I GTAC 3 cut(s) 166, 180, 231
CspCI CAANNNNNGTGG 2 cut(s) 375, 410
CviAII CATG 4 cut(s) 40, 216, 276, 329
CviJI RGCY 7 cut(s) 79, 87, 98, 340, 353, 401, 407
CviKI_1 RGCY 7 cut(s) 79, 87, 98, 340, 353, 401, 407
CviQI GTAC 3 cut(s) 166, 180, 231
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 353
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 2 cut(s) 98, 112
EcoT14I CCWWGG 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 4 cut(s) 43, 219, 279, 332
FaiI YATR 7 cut(s) 26, 41, 217, 236, 277, 330, 344
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 39, 215, 275, 328
Fnu4HI GCNGC 2 cut(s) 223, 248
FokI GGATG 1 cut(s) 303
Fsp4HI GCNGC 2 cut(s) 223, 248
FspBI CTAG 2 cut(s) 74, 404
GlaI GCGC 1 cut(s) 214
GluI GCNGC 2 cut(s) 223, 248
HaeIII GGCC 2 cut(s) 79, 353
HhaI GCGC 1 cut(s) 215
Hin1II CATG 4 cut(s) 43, 219, 279, 332
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 2 cut(s) 202, 325
Hpy8I GTNNAC 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 253, 391
HpyCH4IV ACGT 2 cut(s) 178, 204
HpyCH4V TGCA 3 cut(s) 119, 250, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpySE526I ACGT 2 cut(s) 178, 204
Hsp92II CATG 4 cut(s) 43, 219, 279, 332
HspAI GCGC 1 cut(s) 213
Lsp1109I GCAGC 1 cut(s) 234
MaeI CTAG 2 cut(s) 74, 404
MaeII ACGT 2 cut(s) 178, 204
MaeIII GTNAC 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 106, 121
MluCI AATT 4 cut(s) 20, 57, 378, 391
MnlI CCTC 4 cut(s) 221, 233, 256, 428
MseI TTAA 3 cut(s) 126, 159, 390
MspR9I CCNGG 2 cut(s) 100, 114
MvaI CCWGG 2 cut(s) 100, 114
MwoI GCNNNNNNNGC 1 cut(s) 219
NlaIII CATG 4 cut(s) 43, 219, 279, 332
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 4 cut(s) 43, 219, 279, 332
PaeI GCATGC 1 cut(s) 332
PceI AGGCCT 1 cut(s) 353
PciI ACATGT 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PkrI GCNGC 2 cut(s) 224, 249
PscI ACATGT 1 cut(s) 275
PshBI ATTAAT 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 98, 112
PspGI CCWGG 2 cut(s) 98, 112
PspPI GGNCC 1 cut(s) 77
RsaI GTAC 3 cut(s) 167, 181, 232
RsaNI GTAC 3 cut(s) 166, 180, 231
SaqAI TTAA 3 cut(s) 126, 159, 390
SatI GCNGC 2 cut(s) 223, 248
Sau96I GGNCC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 100, 114
SduI GDGCHC 2 cut(s) 106, 121
SetI ASST 4 cut(s) 118, 181, 207, 232
SfcI CTRYAG 1 cut(s) 234
SphI GCATGC 1 cut(s) 332
Sse9I AATT 4 cut(s) 20, 57, 378, 391
SseBI AGGCCT 1 cut(s) 353
SsiI CCGC 2 cut(s) 222, 225
SspMI CTAG 2 cut(s) 74, 404
StuI AGGCCT 1 cut(s) 353
StyD4I CCNGG 2 cut(s) 98, 112
StyI CCWWGG 1 cut(s) 348
TaiI ACGT 2 cut(s) 181, 207
TaqI TCGA 3 cut(s) 130, 209, 376
TasI AATT 4 cut(s) 20, 57, 378, 391
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 225
Tru1I TTAA 3 cut(s) 126, 159, 390
Tru9I TTAA 3 cut(s) 126, 159, 390
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 13, 161
TspRI CASTG 1 cut(s) 427
VneI GTGCAC 1 cut(s) 117
VspI ATTAAT 1 cut(s) 126
XapI RAATTY 1 cut(s) 20
XceI RCATGY 4 cut(s) 43, 219, 279, 332
XspI CTAG 2 cut(s) 74, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.