RLG00000006733

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016027
Physical Location & Seq
Forward (+)
10034733 .. 10036332
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006733

Sequence Viewer

Length: 1098 bp
ATGAAAACCACACTACTACTACTACTTCTCCTTCTTGTATGTTCTTCATCTGTTTCATCATCAGATTCATCTCTAATATCCAATGCTGTTTGTGGGATCTTCACTTTCGGAGACTCCATCTTCGACGCTGGAAACAATCACTTCAACAAGAACTGCACTGTTCAAGCAGATTTCCCACCGTATGGCTCAAGCTTCTTCCACTACCCGACTGGCAGGTTCACTAATGGCAGAACTGTTGCTGATTTCATCTCACAATTCATCAGCATTCCTCCCCAAACACCATATTATGAGGTACAAATTGAAGCTATGACTGGAAGCCGGAAAGGTTATCCTTCCAATGGCATTAACTTTGCTAGTGCCGGCAGTGGTGTTTTGCAAGGAACAAATAAAAATTTGGGAGTCACCTCACTCCAAGAACAATTACAACAATTCAAGACACTAGTGGAGCAAAACCACATTGACAAAACTCTAGTTCAGAAATCCTTCGTCTTCTTCGAGTCTGGTTCCAACGACATCTTCAACTACTTTCTTCCATTTGCTCCCCCAACACTAGACCCGGATGCCTATGTCCAAGCCATGCTAACCCAAGTCACAAAATTCATAGACCAAATCTACATGCTTGGCGCTCGCCGAATTGCTTTGTTTTCACTAGGGCCTGTTGGCTGTGTTCCAGCCAGGGCACTCCTGCCAGGTGCACCCATTAATCGATGTTTCAGAAAGATGAACTTGATGGTTAAGAAGTACAAGGGACTGGAATGTTTGGTCAACGATATGCCTATCAAGTACCCCGGGGTGGTGGGTGTTTATGGAGAAACATATGACATTGTTCAGCGATTTCGAGCCATTCCTACACGTTACCATTTTTTGGACGTATCGAGCGCATGTTGCGGCGGAGGTTCTCTAGGAGGATTGCTGCAATGTGGGAAGGAGGGATACAAGACATGTGCAAACCCTAATGAGTTCCTATTCTGGGATTACTTCCATCCATCAGAGCATGCTTACAGGCTCATATCCAGGGCCTTGTGGGGGGGAAAGAAACATCGAATTAGACCACTTAATTTGAAGGCACTAGCCAACATCACCCTCACTCGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.44

Weight (kDa)

9.07

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 340 6.7e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016027)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71120
prunus_persica Prupe.1G321500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433751 RchiOBHm_Chr4g0433761
rosa_laevigata RLG00000006733
rosa_multiflora Rmu_sc0006151.1_g000003
rosa_roxburghii Rroxscaffold_5G00375190
rosa_rugosa Rorug04G0271300
rosa_samantha Rh4AG325800 Rh4AG325900 Rh4BG333600 Rh4CG348800 Rh4DG330200 Rh4DG330300
rosa_wichuraiana Rw4G028220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB7I CCANNNNNTGG 2 cut(s) 182, 865
AciI CCGC 2 cut(s) 888, 891
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 2 cut(s) 391, 596
AfaI GTAC 3 cut(s) 294, 743, 785
AfiI CCNNNNNNNGG 6 cut(s) 182, 338, 793, 865, 970, 1026
AflIII ACRYGT 2 cut(s) 851, 941
AgsI TTSAA 6 cut(s) 145, 164, 302, 433, 520, 1063
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 3 cut(s) 674, 688, 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AluBI AGCT 2 cut(s) 192, 305
AluI AGCT 2 cut(s) 192, 305
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 788
AoxI GGCC 2 cut(s) 653, 1017
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 2 cut(s) 391, 596
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AseI ATTAAT 1 cut(s) 702
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 2 cut(s) 626, 881
AspS9I GGNCC 2 cut(s) 653, 1017
AsuC2I CCSGG 3 cut(s) 557, 789, 790
AsuHPI GGTGA 2 cut(s) 394, 1072
AvaI CYCGRG 1 cut(s) 788
BaeGI GKGCMC 2 cut(s) 682, 697
BarI GAAGNNNNNNTAC 2 cut(s) 917, 949
BauI CACGAG 1 cut(s) 1089
BbsI GAAGAC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 1 cut(s) 900
BccI CCATC 4 cut(s) 125, 724, 990, 994
BciT130I CCWGG 3 cut(s) 676, 690, 1015
BciVI GTATCC 1 cut(s) 926
BcnI CCSGG 3 cut(s) 557, 789, 790
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 7 cut(s) 354, 440, 470, 551, 650, 902, 1070
BfoI RGCGCY 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 204
BfuI GTATCC 1 cut(s) 926
BisI GCNGC 2 cut(s) 889, 914
BlsI GCNGC 2 cut(s) 890, 915
Bme1390I CCNGG 6 cut(s) 557, 676, 690, 789, 790, 1015
BmeT110I CYCGRG 1 cut(s) 788
BmgT120I GGNCC 2 cut(s) 653, 1017
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 6 cut(s) 557, 676, 690, 789, 790, 1015
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 481
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 3 cut(s) 557, 789, 790
Bsa29I ATCGAT 1 cut(s) 706
BsaJI CCNNGG 5 cut(s) 675, 787, 788, 789, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 338, 793, 865, 970, 1026
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 214, 316, 756
Bse3DI GCAATG 1 cut(s) 923
BseBI CCWGG 3 cut(s) 676, 690, 1015
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 5 cut(s) 675, 787, 788, 789, 1014
BseGI GGATG 2 cut(s) 565, 982
BseLI CCNNNNNNNGG 6 cut(s) 182, 338, 793, 865, 970, 1026
BseMI GCAATG 1 cut(s) 923
BseNI ACTGG 3 cut(s) 214, 316, 756
BseSI GKGCMC 2 cut(s) 682, 697
BseXI GCAGC 1 cut(s) 900
BsgI GTGCAG 1 cut(s) 139
BshFI GGCC 2 cut(s) 655, 1019
BshVI ATCGAT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 4 cut(s) 319, 360, 557, 789
BslFI GGGAC 1 cut(s) 762
BslI CCNNNNNNNGG 6 cut(s) 182, 338, 793, 865, 970, 1026
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 762
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 2 cut(s) 655, 1019
BsoBI CYCGRG 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 682, 697
Bsp143I GATC 1 cut(s) 96
BspACI CCGC 2 cut(s) 888, 891
BspANI GGCC 2 cut(s) 655, 1019
BspDI ATCGAT 1 cut(s) 706
BspLI GGNNCC 1 cut(s) 505
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 923
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 214, 316, 756
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 5 cut(s) 675, 787, 788, 789, 1014
BssMI GATC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 3 cut(s) 676, 690, 1015
Bst4CI ACNGT 3 cut(s) 160, 180, 235
BstC8I GCNNGC 3 cut(s) 361, 628, 996
BstF5I GGATG 2 cut(s) 565, 982
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 2 cut(s) 626, 881
BstKTI GATC 1 cut(s) 99
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 1 cut(s) 885
BstNI CCWGG 3 cut(s) 676, 690, 1015
BstNSI RCATGY 4 cut(s) 619, 885, 945, 998
BstSCI CCNGG 6 cut(s) 555, 674, 688, 787, 788, 1013
BstSLI GKGCMC 2 cut(s) 682, 697
BstV1I GCAGC 1 cut(s) 900
BstV2I GAAGAC 1 cut(s) 481
BstX2I RGATCY 1 cut(s) 96
BstYI RGATCY 1 cut(s) 96
Bsu15I ATCGAT 1 cut(s) 706
BsuI GTATCC 1 cut(s) 926
BsuRI GGCC 2 cut(s) 655, 1019
BsuTUI ATCGAT 1 cut(s) 706
BtsCI GGATG 2 cut(s) 565, 982
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 2 cut(s) 156, 370
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 361, 628, 996
CfoI GCGC 2 cut(s) 626, 881
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 653, 1017
Cfr9I CCCGGG 1 cut(s) 788
ClaI ATCGAT 1 cut(s) 706
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 293, 742, 784
CspCI CAANNNNNGTGG 2 cut(s) 1041, 1076
CviAII CATG 5 cut(s) 577, 616, 882, 942, 995
CviQI GTAC 3 cut(s) 293, 742, 784
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 788
EcoO109I RGGNCCY 2 cut(s) 653, 1017
EcoRII CCWGG 3 cut(s) 674, 688, 1013
FaeI CATG 5 cut(s) 580, 619, 885, 945, 998
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 762
FatI CATG 5 cut(s) 576, 615, 881, 941, 994
FauNDI CATATG 1 cut(s) 817
Fnu4HI GCNGC 2 cut(s) 889, 914
FokI GGATG 2 cut(s) 572, 969
Fsp4HI GCNGC 2 cut(s) 889, 914
FspBI CTAG 7 cut(s) 354, 440, 470, 551, 650, 902, 1070
GlaI GCGC 2 cut(s) 625, 880
GluI GCNGC 2 cut(s) 889, 914
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 2 cut(s) 655, 1019
HapII CCGG 4 cut(s) 319, 360, 557, 789
HgaI GACGC 1 cut(s) 134
HhaI GCGC 2 cut(s) 626, 881
Hin1II CATG 5 cut(s) 580, 619, 885, 945, 998
Hin6I GCGC 2 cut(s) 624, 879
HinP1I GCGC 2 cut(s) 624, 879
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 190
HinfI GANTC 4 cut(s) 65, 113, 399, 497
HpaII CCGG 4 cut(s) 319, 360, 557, 789
HphI GGTGA 2 cut(s) 394, 1072
Hpy166II GTNNAC 3 cut(s) 219, 695, 766
Hpy188I TCNGA 5 cut(s) 64, 110, 477, 716, 991
Hpy188III TCNNGA 1 cut(s) 433
Hpy8I GTNNAC 3 cut(s) 219, 695, 766
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 41, 342, 493, 919, 1057
HpyCH4III ACNGT 3 cut(s) 160, 180, 235
HpyCH4IV ACGT 2 cut(s) 853, 870
HpyCH4V TGCA 5 cut(s) 156, 376, 695, 916, 947
HpyF10VI GCNNNNNNNGC 1 cut(s) 885
HpySE526I ACGT 2 cut(s) 853, 870
Hsp92II CATG 5 cut(s) 580, 619, 885, 945, 998
HspAI GCGC 2 cut(s) 624, 879
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 445, 544
Lsp1109I GCAGC 1 cut(s) 900
LweI GCATC 1 cut(s) 550
MaeI CTAG 7 cut(s) 354, 440, 470, 551, 650, 902, 1070
MaeII ACGT 2 cut(s) 853, 870
MaeIII GTNAC 3 cut(s) 400, 589, 854
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 8 cut(s) 36, 91, 112, 187, 481, 484, 508, 521
MflI RGATCY 1 cut(s) 96
MhlI GDGCHC 2 cut(s) 682, 697
MluCI AATT 9 cut(s) 254, 297, 391, 419, 428, 596, 633, 1044, 1057
MlyI GAGTC 3 cut(s) 107, 408, 506
MmeI TCCRAC 1 cut(s) 531
MnlI CCTC 7 cut(s) 279, 283, 415, 887, 899, 922, 1094
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 4 cut(s) 345, 702, 735, 1056
MspI CCGG 4 cut(s) 319, 360, 557, 789
MspR9I CCNGG 6 cut(s) 557, 676, 690, 789, 790, 1015
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 3 cut(s) 676, 690, 1015
MwoI GCNNNNNNNGC 1 cut(s) 885
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 3 cut(s) 557, 789, 790
NdeI CATATG 1 cut(s) 817
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 5 cut(s) 580, 619, 885, 945, 998
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 400, 589
NspI RCATGY 4 cut(s) 619, 885, 945, 998
PaeI GCATGC 1 cut(s) 998
PciI ACATGT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PctI GAATGC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 2 cut(s) 182, 865
PkrI GCNGC 2 cut(s) 890, 915
PleI GAGTC 3 cut(s) 107, 407, 505
PpsI GAGTC 3 cut(s) 107, 407, 505
PscI ACATGT 1 cut(s) 941
PshBI ATTAAT 1 cut(s) 702
Psp6I CCWGG 3 cut(s) 674, 688, 1013
PspGI CCWGG 3 cut(s) 674, 688, 1013
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 2 cut(s) 653, 1017
PsuI RGATCY 1 cut(s) 96
RsaI GTAC 3 cut(s) 294, 743, 785
RsaNI GTAC 3 cut(s) 293, 742, 784
SaqAI TTAA 4 cut(s) 345, 702, 735, 1056
SatI GCNGC 2 cut(s) 889, 914
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 2 cut(s) 653, 1017
SchI GAGTC 3 cut(s) 107, 408, 506
ScrFI CCNGG 6 cut(s) 557, 676, 690, 789, 790, 1015
SduI GDGCHC 2 cut(s) 682, 697
SfaNI GCATC 1 cut(s) 550
SmaI CCCGGG 1 cut(s) 790
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
SpeI ACTAGT 1 cut(s) 439
SphI GCATGC 1 cut(s) 998
Sse9I AATT 9 cut(s) 254, 297, 391, 419, 428, 596, 633, 1044, 1057
SsiI CCGC 2 cut(s) 888, 891
SspMI CTAG 7 cut(s) 354, 440, 470, 551, 650, 902, 1070
StyD4I CCNGG 6 cut(s) 555, 674, 688, 787, 788, 1013
TaaI ACNGT 3 cut(s) 160, 180, 235
TaiI ACGT 2 cut(s) 856, 873
TaqI TCGA 6 cut(s) 123, 495, 706, 838, 875, 1042
TasI AATT 9 cut(s) 254, 297, 391, 419, 428, 596, 633, 1044, 1057
TatI WGTACW 1 cut(s) 741
TauI GCSGC 1 cut(s) 891
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 345, 702, 735, 1056
Tru9I TTAA 4 cut(s) 345, 702, 735, 1056
TscAI CASTG 2 cut(s) 163, 370
TseFI GTSAC 2 cut(s) 400, 589
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 2 cut(s) 400, 589
TspDTI ATGAA 8 cut(s) 17, 36, 45, 57, 235, 247, 589, 737
TspMI CCCGGG 1 cut(s) 788
TspRI CASTG 2 cut(s) 163, 370
Van91I CCANNNNNTGG 2 cut(s) 182, 865
VneI GTGCAC 1 cut(s) 693
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 2 cut(s) 391, 596
XceI RCATGY 4 cut(s) 619, 885, 945, 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 788
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 7 cut(s) 354, 440, 470, 551, 650, 902, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.