Prupe.1G533200_v2.0.a1

M-phase-specific PLK1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014057
Physical Location & Seq
Reverse (-)
43592312 .. 43594091
1780 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G533200.1

Sequence Viewer

Length: 1263 bp
ATGGACGAATCTGAGAAAAGAAAGGAACGACTAAGGGCAATGCGCACAGAAGCTGAAGAAGCTGAAGCTTCTCATAGTGTCACAACTTCAGCTGTCCCTGGTTACCTTTCCAATCCGTTGGCTGAAGATAGTGCAGCTTTACCAGTGCACAAGGAACCTTGTGCTCCTTCTAGGTTTGACTTTTACACGGACCCTATGGCAGCCTTCTCTTCTGACACTAAAAGGGTTAAAGTTGGCAACCAGATTGCGCCAAGTAATTTCGGACGTCCAAATACGGGTGGTTCTCCTATGGCAAGGCTTTCGTCACCTCTTTCAGGAGGACCAAGGAACCCTGAAATGACTGCTCCTCCATCCCATCAGTTTCAGAGCAATTATTCACTAGATAAGAGAATGTATCGAGTACAACAAGGTTTCTGTCAAAACTTTGGTCCTCAAAGAAACCCCATAGGAATAGCAAGACCATTCCCTATGCATCATGGAAATCCACCTGAAGTCTGGAATGGAGCTGAAGGTGCTGCCAATTATAGTTTTCCATCTGATCCTTCAAGAGAATGCAGGTTTCCTGGCCCTGGGTTTAGACCACCAGGAAGCCCCGGATTTAGACCACCAGGAAGCCCTGGACTTGGACCACAAGGAAGCTCTGGATTTGGACCACCAGGAAGCCCTGGATTTAGACCACCAGGAAGTCCTGGATTTAGACCACCAGGAAGCCCTGGATTTGGACCACAAGGAAGCTCTGGATTTGGACCACCAGGAAGCCCTGGATTTAGACCACCTGCAAGCCCGGGATTTAGACCGCTAGGAAGCCCTGGTTCCAATAGTGGGCAAGGCAGGGGGCACTGGCGTAGCAATAGTCCAAGCCCTCATTCAGTACATGGAGGCAATACTAGTCCTAGTTCAAGTTCAGGAAGAGGTGGGGGGCACTGGAGCACAAGTCCTGGATCGGGACGGAGAGGTGGAAGAGGGCTGGGCTCTCATGGTCGTTCTACAATGGAAAAACAATTGGGGCCAGAGCGGTATTACAATGATTCCATGGTTGAAGACCCATGGAAGTTTTTGAAACCCGTTATCTGGAAAGGTGTAGATACTCCCATGAAACGTTTCTACAGCCCTGGCTCTTCAAAACCCCCAATTGAAAATTCTAGTAGCACAAAAGATGCTATCATTTCAGAAGGCTCAAACAAGTCCACTTCACAGCCAAGCTTGGCGGAGTACTTGGCCGCCTCATTCAATGATGCTGTCAAGGACACACCAACTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000381 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006970 GO:0007623 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009605 GO:0009628 GO:0009649 GO:0009651 GO:0009889 GO:0009892 GO:0009914 GO:0009926 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010817 GO:0010866 GO:0010921 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0019216 GO:0019219 GO:0019220 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035303 GO:0040029 GO:0042221 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046890 GO:0048024 GO:0048511 GO:0048519 GO:0050684 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051174 GO:0051179 GO:0051234 GO:0051252 GO:0051336 GO:0051716 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070887 GO:0070918 GO:0071071 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071944 GO:0080090 GO:0090207 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1901703 GO:1903311 GO:1903725 GO:1903730 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPLKIP

Protein Analysis

421

Amino Acids

44.33

Weight (kDa)

9.8

Isoelectric Point (pI)

65.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24500 AT4G24500 AT4G24500
fragaria_vesca FvH4_5g34870
malus_domestica MD08G1199700.v1.1
prunus_persica Prupe.1G533200_v2.0.a1
pyrus_communis pycom08g17330 pycom15g34700
rosa_chinensis RchiOBHm_Chr7g0235791
rosa_laevigata RLG00000001139
rosa_roxburghii Rroxscaffold_3G00225630
rosa_rugosa Rorug07G0292800
rosa_samantha Rh7AG447700 Rh7BG419900 Rh7CG468200 Rh7DG436700
rosa_wichuraiana Rw7G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
AatII GACGTC 1 cut(s) 268
Acc16I TGCGCA 1 cut(s) 44
Acc36I ACCTGC 2 cut(s) 546, 784
AccBSI CCGCTC 1 cut(s) 1015
AciI CCGC 4 cut(s) 797, 1015, 1208, 1221
AclI AACGTT 1 cut(s) 1099
AclWI GGATC 2 cut(s) 533, 949
AcoI YGGCCR 1 cut(s) 1218
AcsI RAATTY 1 cut(s) 1138
AcuI CTGAAG 6 cut(s) 72, 75, 84, 144, 510, 528
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 3 cut(s) 402, 873, 1214
AfiI CCNNNNNNNGG 8 cut(s) 275, 314, 569, 623, 719, 822, 944, 1071
AgsI TTSAA 7 cut(s) 546, 900, 1040, 1060, 1122, 1136, 1231
AhlI ACTAGT 1 cut(s) 887
AjuI GAANNNNNNNTTGG 4 cut(s) 850, 882, 1123, 1155
AluBI AGCT 9 cut(s) 53, 62, 68, 92, 137, 506, 639, 735, 1203
AluI AGCT 9 cut(s) 53, 62, 68, 92, 137, 506, 639, 735, 1203
Alw21I GWGCWC 3 cut(s) 150, 166, 932
Alw44I GTGCAC 1 cut(s) 146
AlwI GGATC 2 cut(s) 533, 949
AlwNI CAGNNNCTG 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 784
AoxI GGCC 3 cut(s) 565, 1007, 1218
ApaLI GTGCAC 1 cut(s) 146
ApeKI GCWGC 3 cut(s) 134, 200, 515
ApoI RAATTY 1 cut(s) 1138
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
Asp700I GAANNNNTTC 1 cut(s) 1100
AspLEI GCGC 2 cut(s) 45, 250
AspS9I GGNCC 9 cut(s) 190, 320, 428, 566, 626, 650, 722, 746, 1007
AsuC2I CCSGG 3 cut(s) 594, 785, 786
AsuHPI GGTGA 1 cut(s) 297
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 7 cut(s) 190, 320, 428, 626, 650, 722, 746
BaeGI GKGCMC 3 cut(s) 150, 840, 924
BanII GRGCYC 1 cut(s) 974
BbsI GAAGAC 1 cut(s) 1047
Bbv12I GWGCWC 3 cut(s) 150, 166, 932
BbvI GCAGC 3 cut(s) 146, 212, 502
BccI CCATC 3 cut(s) 358, 363, 541
BcgI CGANNNNNNTGC 2 cut(s) 282, 316
BcnI CCSGG 3 cut(s) 594, 785, 786
BcuI ACTAGT 1 cut(s) 887
BfaI CTAG 6 cut(s) 171, 380, 800, 888, 894, 1143
BfmI CTRYAG 1 cut(s) 1105
BfuAI ACCTGC 2 cut(s) 546, 784
BisI GCNGC 4 cut(s) 135, 201, 516, 1221
BlsI GCNGC 4 cut(s) 136, 202, 517, 1222
BmcAI AGTACT 1 cut(s) 1214
Bme18I GGWCC 7 cut(s) 190, 320, 428, 626, 650, 722, 746
BmeT110I CYCGRG 1 cut(s) 784
BmgT120I GGNCC 9 cut(s) 190, 320, 428, 566, 626, 650, 722, 746, 1007
BmiI GGNNCC 5 cut(s) 156, 192, 329, 814, 1008
BmsI GCATC 3 cut(s) 481, 1147, 1225
BpiI GAAGAC 1 cut(s) 1047
BpmI CTGGAG 1 cut(s) 946
BpuMI CCSGG 3 cut(s) 594, 785, 786
BsaHI GRCGYC 1 cut(s) 265
BsaXI ACNNNNNCTCC 4 cut(s) 331, 361, 919, 949
Bsc4I CCNNNNNNNGG 8 cut(s) 275, 314, 569, 623, 719, 822, 944, 1071
Bse1I ACTGG 3 cut(s) 143, 845, 929
Bse3DI GCAATG 1 cut(s) 45
BseGI GGATG 1 cut(s) 350
BseLI CCNNNNNNNGG 8 cut(s) 275, 314, 569, 623, 719, 822, 944, 1071
BseMI GCAATG 1 cut(s) 45
BseNI ACTGG 3 cut(s) 143, 845, 929
BseRI GAGGAG 1 cut(s) 336
BseSI GKGCMC 3 cut(s) 150, 840, 924
BseXI GCAGC 3 cut(s) 146, 212, 502
BseYI CCCAGC 1 cut(s) 967
BsgI GTGCAG 1 cut(s) 153
BshFI GGCC 3 cut(s) 567, 1009, 1220
BsiHKAI GWGCWC 3 cut(s) 150, 166, 932
BsiHKCI CYCGRG 1 cut(s) 784
BsiSI CCGG 2 cut(s) 594, 785
BslFI GGGAC 2 cut(s) 80, 960
BslI CCNNNNNNNGG 8 cut(s) 275, 314, 569, 623, 719, 822, 944, 1071
BsmFI GGGAC 2 cut(s) 80, 960
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 3 cut(s) 567, 1009, 1220
BsoBI CYCGRG 1 cut(s) 784
Bsp1286I GDGCHC 6 cut(s) 150, 166, 840, 924, 932, 974
Bsp143I GATC 2 cut(s) 538, 941
Bsp19I CCATGG 2 cut(s) 1032, 1046
BspACI CCGC 4 cut(s) 797, 1015, 1208, 1221
BspANI GGCC 3 cut(s) 567, 1009, 1220
BspCNI CTCAG 1 cut(s) 4
BspLI GGNNCC 5 cut(s) 156, 192, 329, 814, 1008
BspMI ACCTGC 2 cut(s) 546, 784
BspPI GGATC 2 cut(s) 533, 949
BspQI GCTCTTC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 1015
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 3 cut(s) 143, 845, 929
BssMI GATC 2 cut(s) 538, 941
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 3 cut(s) 323, 1032, 1046
Bst6I CTCTTC 4 cut(s) 214, 904, 955, 1123
BstACI GRCGYC 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 781
BstDEI CTNAG 2 cut(s) 12, 32
BstDSI CCRYGG 2 cut(s) 1032, 1046
BstEII GGTNACC 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 350
BstHHI GCGC 2 cut(s) 45, 250
BstKTI GATC 2 cut(s) 541, 944
BstMBI GATC 2 cut(s) 538, 941
BstMWI GCNNNNNNNGC 2 cut(s) 59, 512
BstPI GGTNACC 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 1105
BstSLI GKGCMC 3 cut(s) 150, 840, 924
BstV1I GCAGC 3 cut(s) 146, 212, 502
BstV2I GAAGAC 1 cut(s) 1047
BstXI CCANNNNNNTGG 1 cut(s) 118
BsuRI GGCC 3 cut(s) 567, 1009, 1220
BtgI CCRYGG 2 cut(s) 1032, 1046
BtsCI GGATG 1 cut(s) 350
BtsIMutI CAGTG 3 cut(s) 150, 838, 922
BveI ACCTGC 2 cut(s) 546, 784
Cac8I GCNNGC 1 cut(s) 781
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 2 cut(s) 45, 250
Cfr13I GGNCC 9 cut(s) 190, 320, 428, 566, 626, 650, 722, 746, 1007
Cfr9I CCCGGG 1 cut(s) 784
Csp6I GTAC 3 cut(s) 401, 872, 1213
CviAII CATG 6 cut(s) 476, 875, 977, 1033, 1047, 1093
CviQI GTAC 3 cut(s) 401, 872, 1213
DdeI CTNAG 2 cut(s) 12, 32
DpnI GATC 2 cut(s) 540, 943
DpnII GATC 2 cut(s) 538, 941
EaeI YGGCCR 1 cut(s) 1218
Eam1104I CTCTTC 4 cut(s) 214, 904, 955, 1123
EarI CTCTTC 4 cut(s) 214, 904, 955, 1123
EciI GGCGGA 1 cut(s) 1223
Eco130I CCWWGG 3 cut(s) 323, 1032, 1046
Eco24I GRGCYC 1 cut(s) 974
Eco47I GGWCC 7 cut(s) 190, 320, 428, 626, 650, 722, 746
Eco57I CTGAAG 6 cut(s) 72, 75, 84, 144, 510, 528
Eco88I CYCGRG 1 cut(s) 784
Eco91I GGTNACC 1 cut(s) 101
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoO65I GGTNACC 1 cut(s) 101
EcoT14I CCWWGG 3 cut(s) 323, 1032, 1046
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 974
ErhI CCWWGG 3 cut(s) 323, 1032, 1046
FaeI CATG 6 cut(s) 479, 878, 980, 1036, 1050, 1096
FaqI GGGAC 2 cut(s) 80, 960
FatI CATG 6 cut(s) 475, 874, 976, 1032, 1046, 1092
Fnu4HI GCNGC 4 cut(s) 135, 201, 516, 1221
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 974
Fsp4HI GCNGC 4 cut(s) 135, 201, 516, 1221
FspAI RTGCGCAY 1 cut(s) 44
FspBI CTAG 6 cut(s) 171, 380, 800, 888, 894, 1143
FspI TGCGCA 1 cut(s) 44
GlaI GCGC 2 cut(s) 44, 249
GluI GCNGC 4 cut(s) 135, 201, 516, 1221
GsaI CCCAGC 1 cut(s) 971
GsuI CTGGAG 1 cut(s) 946
HaeIII GGCC 3 cut(s) 567, 1009, 1220
HapII CCGG 2 cut(s) 594, 785
HhaI GCGC 2 cut(s) 45, 250
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 6 cut(s) 479, 878, 980, 1036, 1050, 1096
Hin6I GCGC 2 cut(s) 43, 248
HinP1I GCGC 2 cut(s) 43, 248
HindIII AAGCTT 2 cut(s) 66, 1201
HinfI GANTC 2 cut(s) 8, 1028
HpaII CCGG 2 cut(s) 594, 785
HphI GGTGA 1 cut(s) 297
Hpy166II GTNNAC 2 cut(s) 148, 1188
Hpy188I TCNGA 6 cut(s) 13, 214, 263, 366, 538, 1171
Hpy188III TCNNGA 8 cut(s) 315, 496, 546, 642, 738, 906, 945, 1072
Hpy8I GTNNAC 2 cut(s) 148, 1188
HpyAV CCTTC 5 cut(s) 177, 214, 503, 552, 1166
HpyCH4IV ACGT 2 cut(s) 265, 1099
HpyCH4V TGCA 5 cut(s) 134, 148, 472, 555, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 512
HpyF3I CTNAG 2 cut(s) 12, 32
HpySE526I ACGT 2 cut(s) 265, 1099
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 6 cut(s) 479, 878, 980, 1036, 1050, 1096
HspAI GCGC 2 cut(s) 43, 248
Kzo9I GATC 2 cut(s) 538, 941
LguI GCTCTTC 1 cut(s) 1123
LmnI GCTCC 4 cut(s) 169, 349, 503, 927
Lsp1109I GCAGC 3 cut(s) 146, 212, 502
LweI GCATC 3 cut(s) 481, 1147, 1225
MaeI CTAG 6 cut(s) 171, 380, 800, 888, 894, 1143
MaeII ACGT 2 cut(s) 265, 1099
MaeIII GTNAC 3 cut(s) 79, 101, 303
MalI GATC 2 cut(s) 540, 943
MbiI CCGCTC 1 cut(s) 1015
MboI GATC 2 cut(s) 538, 941
MboII GAAGA 7 cut(s) 68, 137, 201, 921, 972, 1052, 1110
MfeI CAATTG 2 cut(s) 1001, 1131
MhlI GDGCHC 6 cut(s) 150, 166, 840, 924, 932, 974
MluCI AATT 6 cut(s) 256, 370, 520, 1001, 1131, 1138
Mph1103I ATGCAT 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 1100
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 594, 785
MunI CAATTG 2 cut(s) 1001, 1131
Mva1269I GAATGC 1 cut(s) 557
MwoI GCNNNNNNNGC 2 cut(s) 59, 512
NciI CCSGG 3 cut(s) 594, 785, 786
NcoI CCATGG 2 cut(s) 1032, 1046
NdeII GATC 2 cut(s) 538, 941
NlaIII CATG 6 cut(s) 479, 878, 980, 1036, 1050, 1096
NlaIV GGNNCC 5 cut(s) 156, 192, 329, 814, 1008
NmuCI GTSAC 2 cut(s) 79, 303
NsbI TGCGCA 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 474
PaqCI CACCTGC 1 cut(s) 784
PasI CCCWGGG 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 1123
PctI GAATGC 1 cut(s) 557
PdmI GAANNNNTTC 1 cut(s) 1100
PfeI GAWTC 2 cut(s) 8, 1028
PfoI TCCNGGA 2 cut(s) 688, 937
PkrI GCNGC 4 cut(s) 136, 202, 517, 1222
Psp1406I AACGTT 1 cut(s) 1099
PspEI GGTNACC 1 cut(s) 101
PspFI CCCAGC 1 cut(s) 967
PspN4I GGNNCC 5 cut(s) 156, 192, 329, 814, 1008
PspPI GGNCC 9 cut(s) 190, 320, 428, 566, 626, 650, 722, 746, 1007
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 3 cut(s) 402, 873, 1214
RsaNI GTAC 3 cut(s) 401, 872, 1213
SapI GCTCTTC 1 cut(s) 1123
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 135, 201, 516, 1221
Sau3AI GATC 2 cut(s) 538, 941
Sau96I GGNCC 9 cut(s) 190, 320, 428, 566, 626, 650, 722, 746, 1007
ScaI AGTACT 1 cut(s) 1214
SduI GDGCHC 6 cut(s) 150, 166, 840, 924, 932, 974
SfaNI GCATC 3 cut(s) 481, 1147, 1225
SfcI CTRYAG 1 cut(s) 1105
SinI GGWCC 7 cut(s) 190, 320, 428, 626, 650, 722, 746
SmaI CCCGGG 1 cut(s) 786
SpeI ACTAGT 1 cut(s) 887
Sse9I AATT 6 cut(s) 256, 370, 520, 1001, 1131, 1138
SsiI CCGC 4 cut(s) 797, 1015, 1208, 1221
SspMI CTAG 6 cut(s) 171, 380, 800, 888, 894, 1143
StyI CCWWGG 3 cut(s) 323, 1032, 1046
TaiI ACGT 2 cut(s) 268, 1102
TaqI TCGA 1 cut(s) 397
TasI AATT 6 cut(s) 256, 370, 520, 1001, 1131, 1138
TatI WGTACW 3 cut(s) 400, 871, 1212
TauI GCSGC 1 cut(s) 1223
TfiI GAWTC 2 cut(s) 8, 1028
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 3 cut(s) 150, 845, 929
TseFI GTSAC 2 cut(s) 79, 303
TseI GCWGC 3 cut(s) 134, 200, 515
Tsp45I GTSAC 2 cut(s) 79, 303
TspDTI ATGAA 1 cut(s) 1109
TspGWI ACGGA 3 cut(s) 105, 203, 964
TspMI CCCGGG 1 cut(s) 784
TspRI CASTG 3 cut(s) 150, 845, 929
VneI GTGCAC 1 cut(s) 146
VpaK11BI GGWCC 7 cut(s) 190, 320, 428, 626, 650, 722, 746
XagI CCTNNNNNAGG 1 cut(s) 312
XapI RAATTY 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmaI CCCGGG 1 cut(s) 784
XmnI GAANNNNTTC 1 cut(s) 1100
XspI CTAG 6 cut(s) 171, 380, 800, 888, 894, 1143
ZraI GACGTC 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 1214
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.