pycom08g17330

M-phase-specific PLK1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014057
Physical Location & Seq
Reverse (-)
17528386 .. 17529874
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17330.1

Sequence Viewer

Length: 1038 bp
ATGCGCATCGAAGCTGAAGAGACGGAAGCTTCACTTAATGCTGCAACTTCAGCCGTCCCTGGTTACCTTTCCAATCCATTGGCCGAAGATACTACAGCTATACCAGTCCCGGAGGAGCCCTGTGCTCCTTTTAGGTTTGACTTTTACTCAGATCCTATGGCAGCCTTCTCTTCCGACAATAAAAGGATTAAAGTCGGCGACCAGATTGCACAAGATAATTTTAGACATTCAAATACTGGTGGGTTTCCGGGGGCAAGGCTTCCGTCACCTCTTTCAGGAGGACCAAGGAACCCTCAAATGACTGCTTCTCCTGCTCATCAGTTTCAAAGAAGTTATTCACCTGACCAGAGAATGTACCAAGCACAAGGTTCCTATCAAAATTTTAGTCCTCAAAGAAGCCCTGTAGGAATGGAAAGACCTTTCCCTATGCATCACGGGAATCGACCTGAAGTTTGGAATGGAGCTGAATTTAGACCACCAGCAAGCCCTGGATATGGACCACAAGGAAGCCCTAGATTTAGGCCACAAGGAAGCCCGGGATTTAGACCACCAGGAAGCCCTAGATTTCAACCACCTGGAAGCCCTGGATTTAGACCACCGACAAGCCCTGGATTTAGGCCACCAGGAAGCCCTGGTTCCAATATTGGGCAAGGCAGGGGGCACTGGTTCAGCCATACTCCAAGACCTCAATCAGTACATGGAGGCAGCCCTAGTCCTGGTTCAAGTTCAGGAAGAGGTGGGTGGCGTGGTTCTCATGGTCGTGCAATGGACAGACAATTAGGGCCAGAGCGGTTTTACAATGCTTCCATGGTTGAAGACCCATGGAAATTTCTAGAACCTGTAATCTGGAAGGGGGTAGATAATCCCTTGAGATGTTTGAACACCCATGGCTCTTCAAAACCGTCAATTGGTAGATCTAGTAGCACAAATAATGCAAGCATTTCAGAAGCTTTAAACAAGTCCATGCCGCAGCCAAGCTTGGCAGAGTTCTTGGCCGCCTCATTAAATGAAGCTGTCGATGATGCACCAAGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000381 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006970 GO:0007623 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009605 GO:0009628 GO:0009649 GO:0009651 GO:0009889 GO:0009892 GO:0009914 GO:0009926 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010817 GO:0010866 GO:0010921 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0019216 GO:0019219 GO:0019220 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035303 GO:0040029 GO:0042221 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046890 GO:0048024 GO:0048511 GO:0048519 GO:0050684 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051174 GO:0051179 GO:0051234 GO:0051252 GO:0051336 GO:0051716 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070887 GO:0070918 GO:0071071 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071944 GO:0080090 GO:0090207 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1901703 GO:1903311 GO:1903725 GO:1903730 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPLKIP

Protein Analysis

346

Amino Acids

37.19

Weight (kDa)

9.38

Isoelectric Point (pI)

69.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPLKIP PF15502 247 - 289 4.5e-06 M-phase-specific PLK1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24500 AT4G24500 AT4G24500
fragaria_vesca FvH4_5g34870
malus_domestica MD08G1199700.v1.1
prunus_persica Prupe.1G533200_v2.0.a1
pyrus_communis pycom08g17330 pycom15g34700
rosa_chinensis RchiOBHm_Chr7g0235791
rosa_laevigata RLG00000001139
rosa_roxburghii Rroxscaffold_3G00225630
rosa_rugosa Rorug07G0292800
rosa_samantha Rh7AG447700 Rh7BG419900 Rh7CG468200 Rh7DG436700
rosa_wichuraiana Rw7G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccBSI CCGCTC 1 cut(s) 790
AciI CCGC 3 cut(s) 790, 968, 996
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 2 cut(s) 81, 993
AcsI RAATTY 3 cut(s) 379, 467, 827
AcuI CTGAAG 3 cut(s) 33, 36, 468
AfaI GTAC 3 cut(s) 356, 696, 1033
AfiI CCNNNNNNNGG 5 cut(s) 275, 494, 645, 716, 908
AgsI TTSAA 7 cut(s) 231, 326, 569, 723, 815, 880, 897
AjnI CCWGG 9 cut(s) 58, 487, 550, 574, 583, 607, 622, 631, 715
AluBI AGCT 7 cut(s) 14, 29, 98, 464, 950, 978, 1013
AluI AGCT 7 cut(s) 14, 29, 98, 464, 950, 978, 1013
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 5 cut(s) 81, 521, 617, 782, 993
ApeKI GCWGC 4 cut(s) 41, 161, 705, 970
ApoI RAATTY 3 cut(s) 379, 467, 827
ArsI GACNNNNNNTTYG 4 cut(s) 370, 402, 435, 467
Asp700I GAANNNNTTC 1 cut(s) 334
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 281, 497, 782
AsuC2I CCSGG 4 cut(s) 110, 249, 536, 537
AsuHPI GGTGA 2 cut(s) 258, 330
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 2 cut(s) 281, 497
BaeGI GKGCMC 1 cut(s) 663
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 4 cut(s) 28, 173, 717, 982
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 9 cut(s) 60, 489, 552, 576, 585, 609, 624, 633, 717
BcnI CCSGG 4 cut(s) 110, 249, 536, 537
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 5 cut(s) 513, 561, 711, 833, 918
BfmI CTRYAG 2 cut(s) 93, 402
BglII AGATCT 1 cut(s) 914
BisI GCNGC 6 cut(s) 42, 162, 706, 968, 971, 996
BlsI GCNGC 6 cut(s) 43, 163, 707, 969, 972, 997
Bme18I GGWCC 2 cut(s) 281, 497
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 3 cut(s) 281, 497, 782
BmiI GGNNCC 4 cut(s) 117, 290, 370, 637
BmsI GCATC 3 cut(s) 15, 439, 1012
BpiI GAAGAC 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 889
BpuMI CCSGG 4 cut(s) 110, 249, 536, 537
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 5 cut(s) 275, 494, 645, 716, 908
Bse1I ACTGG 3 cut(s) 104, 241, 668
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 9 cut(s) 60, 489, 552, 576, 585, 609, 624, 633, 717
BseLI CCNNNNNNNGG 5 cut(s) 275, 494, 645, 716, 908
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 3 cut(s) 104, 241, 668
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 4 cut(s) 28, 173, 717, 982
BshFI GGCC 5 cut(s) 83, 523, 619, 784, 995
BsiHKAI GWGCWC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 535
BsiSI CCGG 3 cut(s) 110, 248, 536
BslFI GGGAC 2 cut(s) 41, 92
BslI CCNNNNNNNGG 5 cut(s) 275, 494, 645, 716, 908
BsmAI GTCTC 1 cut(s) 14
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 2 cut(s) 41, 92
BsnI GGCC 5 cut(s) 83, 523, 619, 784, 995
BsoBI CYCGRG 1 cut(s) 535
Bsp1286I GDGCHC 3 cut(s) 120, 127, 663
Bsp143I GATC 2 cut(s) 151, 914
Bsp19I CCATGG 3 cut(s) 807, 821, 886
BspACI CCGC 3 cut(s) 790, 968, 996
BspANI GGCC 5 cut(s) 83, 523, 619, 784, 995
BspCNI CTCAG 1 cut(s) 161
BspLI GGNNCC 4 cut(s) 117, 290, 370, 637
BspPI GGATC 1 cut(s) 146
BspQI GCTCTTC 1 cut(s) 898
BsrBI CCGCTC 1 cut(s) 790
BsrDI GCAATG 1 cut(s) 771
BsrI ACTGG 3 cut(s) 104, 241, 668
BssMI GATC 2 cut(s) 151, 914
BssT1I CCWWGG 4 cut(s) 284, 807, 821, 886
Bst2UI CCWGG 9 cut(s) 60, 489, 552, 576, 585, 609, 624, 633, 717
Bst4CI ACNGT 1 cut(s) 903
Bst6I CTCTTC 4 cut(s) 12, 175, 727, 898
BstC8I GCNNGC 2 cut(s) 484, 937
BstDEI CTNAG 1 cut(s) 148
BstDSI CCRYGG 3 cut(s) 807, 821, 886
BstEII GGTNACC 1 cut(s) 62
BstENI CCTNNNNNAGG 1 cut(s) 273
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 2 cut(s) 154, 917
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 2 cut(s) 151, 914
BstMWI GCNNNNNNNGC 2 cut(s) 50, 311
BstNI CCWGG 9 cut(s) 60, 489, 552, 576, 585, 609, 624, 633, 717
BstPI GGTNACC 1 cut(s) 62
BstSFI CTRYAG 2 cut(s) 93, 402
BstSLI GKGCMC 1 cut(s) 663
BstV1I GCAGC 4 cut(s) 28, 173, 717, 982
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 2 cut(s) 151, 914
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 2 cut(s) 151, 914
BsuRI GGCC 5 cut(s) 83, 523, 619, 784, 995
BtgI CCRYGG 3 cut(s) 807, 821, 886
BtsIMutI CAGTG 1 cut(s) 661
Cac8I GCNNGC 2 cut(s) 484, 937
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 3 cut(s) 281, 497, 782
Cfr9I CCCGGG 1 cut(s) 535
Csp6I GTAC 3 cut(s) 355, 695, 1032
CviAII CATG 6 cut(s) 698, 755, 808, 822, 887, 964
CviQI GTAC 3 cut(s) 355, 695, 1032
DdeI CTNAG 1 cut(s) 148
DpnI GATC 2 cut(s) 153, 916
DpnII GATC 2 cut(s) 151, 914
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 2 cut(s) 81, 993
Eam1104I CTCTTC 4 cut(s) 12, 175, 727, 898
EarI CTCTTC 4 cut(s) 12, 175, 727, 898
Eco130I CCWWGG 4 cut(s) 284, 807, 821, 886
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 2 cut(s) 281, 497
Eco57I CTGAAG 3 cut(s) 33, 36, 468
Eco88I CYCGRG 1 cut(s) 535
Eco91I GGTNACC 1 cut(s) 62
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 9 cut(s) 58, 487, 550, 574, 583, 607, 622, 631, 715
EcoT14I CCWWGG 4 cut(s) 284, 807, 821, 886
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 4 cut(s) 284, 807, 821, 886
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 6 cut(s) 701, 758, 811, 825, 890, 967
FalI AAGNNNNNCTT 2 cut(s) 18, 50
FaqI GGGAC 2 cut(s) 41, 92
FatI CATG 6 cut(s) 697, 754, 807, 821, 886, 963
Fnu4HI GCNGC 6 cut(s) 42, 162, 706, 968, 971, 996
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 6 cut(s) 42, 162, 706, 968, 971, 996
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 5 cut(s) 513, 561, 711, 833, 918
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 6 cut(s) 42, 162, 706, 968, 971, 996
HaeIII GGCC 5 cut(s) 83, 523, 619, 784, 995
HapII CCGG 3 cut(s) 110, 248, 536
HhaI GCGC 1 cut(s) 6
Hin1II CATG 6 cut(s) 701, 758, 811, 825, 890, 967
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HindIII AAGCTT 3 cut(s) 27, 948, 976
HinfI GANTC 1 cut(s) 439
HpaII CCGG 3 cut(s) 110, 248, 536
HphI GGTGA 2 cut(s) 258, 330
Hpy188I TCNGA 3 cut(s) 151, 175, 946
Hpy188III TCNNGA 4 cut(s) 276, 729, 833, 847
HpyAV CCTTC 2 cut(s) 175, 844
HpyCH4III ACNGT 1 cut(s) 903
HpyCH4V TGCA 6 cut(s) 44, 209, 430, 764, 935, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 311
HpyF3I CTNAG 1 cut(s) 148
Hsp92II CATG 6 cut(s) 701, 758, 811, 825, 890, 967
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 151, 914
LguI GCTCTTC 1 cut(s) 898
LmnI GCTCC 3 cut(s) 115, 130, 461
Lsp1109I GCAGC 4 cut(s) 28, 173, 717, 982
LweI GCATC 3 cut(s) 15, 439, 1012
MaeI CTAG 5 cut(s) 513, 561, 711, 833, 918
MaeIII GTNAC 2 cut(s) 62, 264
MalI GATC 2 cut(s) 153, 916
MbiI CCGCTC 1 cut(s) 790
MboI GATC 2 cut(s) 151, 914
MboII GAAGA 6 cut(s) 29, 98, 162, 744, 827, 885
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 2 cut(s) 151, 914
MhlI GDGCHC 3 cut(s) 120, 127, 663
MluCI AATT 6 cut(s) 217, 379, 467, 776, 827, 906
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 9 cut(s) 106, 272, 279, 303, 399, 695, 696, 728, 1009
Mph1103I ATGCAT 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 334
MseI TTAA 4 cut(s) 36, 189, 953, 1004
MslI CAYNNNNRTG 1 cut(s) 759
MspI CCGG 3 cut(s) 110, 248, 536
MunI CAATTG 1 cut(s) 906
MvaI CCWGG 9 cut(s) 60, 489, 552, 576, 585, 609, 624, 633, 717
MwoI GCNNNNNNNGC 2 cut(s) 50, 311
NciI CCSGG 4 cut(s) 110, 249, 536, 537
NcoI CCATGG 3 cut(s) 807, 821, 886
NdeII GATC 2 cut(s) 151, 914
NlaIII CATG 6 cut(s) 701, 758, 811, 825, 890, 967
NlaIV GGNNCC 4 cut(s) 117, 290, 370, 637
NmuCI GTSAC 1 cut(s) 264
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 6 cut(s) 43, 163, 707, 969, 972, 997
Psp6I CCWGG 9 cut(s) 58, 487, 550, 574, 583, 607, 622, 631, 715
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 9 cut(s) 58, 487, 550, 574, 583, 607, 622, 631, 715
PspN4I GGNNCC 4 cut(s) 117, 290, 370, 637
PspPI GGNCC 3 cut(s) 281, 497, 782
PsuI RGATCY 2 cut(s) 151, 914
RsaI GTAC 3 cut(s) 356, 696, 1033
RsaNI GTAC 3 cut(s) 355, 695, 1032
RseI CAYNNNNRTG 1 cut(s) 759
SapI GCTCTTC 1 cut(s) 898
SaqAI TTAA 4 cut(s) 36, 189, 953, 1004
SatI GCNGC 6 cut(s) 42, 162, 706, 968, 971, 996
Sau3AI GATC 2 cut(s) 151, 914
Sau96I GGNCC 3 cut(s) 281, 497, 782
SduI GDGCHC 3 cut(s) 120, 127, 663
SfaNI GCATC 3 cut(s) 15, 439, 1012
SfcI CTRYAG 2 cut(s) 93, 402
SinI GGWCC 2 cut(s) 281, 497
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
Sse9I AATT 6 cut(s) 217, 379, 467, 776, 827, 906
SsiI CCGC 3 cut(s) 790, 968, 996
SspI AATATT 1 cut(s) 643
SspMI CTAG 5 cut(s) 513, 561, 711, 833, 918
StyI CCWWGG 4 cut(s) 284, 807, 821, 886
TaaI ACNGT 1 cut(s) 903
TaqI TCGA 3 cut(s) 9, 442, 1017
TasI AATT 6 cut(s) 217, 379, 467, 776, 827, 906
TatI WGTACW 2 cut(s) 694, 1031
TauI GCSGC 2 cut(s) 970, 998
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 4 cut(s) 36, 189, 953, 1004
Tru9I TTAA 4 cut(s) 36, 189, 953, 1004
TscAI CASTG 1 cut(s) 668
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 4 cut(s) 41, 161, 705, 970
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 1 cut(s) 1023
TspGWI ACGGA 2 cut(s) 38, 252
TspMI CCCGGG 1 cut(s) 535
TspRI CASTG 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 281, 497
XagI CCTNNNNNAGG 1 cut(s) 273
XapI RAATTY 3 cut(s) 379, 467, 827
XbaI TCTAGA 1 cut(s) 832
XmaI CCCGGG 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 334
XspI CTAG 5 cut(s) 513, 561, 711, 833, 918
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.