Rorug07G0292800

M-phase-specific PLK1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014057
Physical Location & Seq
Forward (+)
28612394 .. 28613376
983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0292800.1

Sequence Viewer

Length: 648 bp
ATGGATAGAAGTGATTCAGCCCCAATGATCGGCATTCACAGAGAGTCATCATCGTCAAGAAGTAAGGTTGCTGCCAAAGGAGAAGCGTATCATGTCATGAGGGTACTAGCTGCTGATCCCAAGCACGATCATGATAAGGGAGCTGGATGGAAGAAAGGTGTTGCCGTGCTCGACTTCATTCTAAGGCTAGGCGCCACTGCAGCTGCTCTAGGCGCTGCCGTGACCATGGCGTCCAGCGAGCAGACTCTTCCGTTCTTCACGCAGTTCTTCCAGTTTTCAGCTGGTTATGATGACATGCCCACTTTCATGTTTTTTGTGATAGCAATGGCCATAGTGAGTGGCTACCTAGTCCTCTCCCTCCCCTTCTCCATAGTCACCATTGTCCGTCCCCAGTTAGTTGCTCCGAGGCTTCTCTTGCTTATTTTCGACACTCTGGCGCTTACTCTGAACACCTCTGCGGCTGCTGCCGCTGCTGCCATAGTTTACTTGGCTCACAATGGGAACTCGGATGCAAATTGGCTTGCTATTTGCCAACAGTTTACTGACTTCTGCCAGCAAACCAGCGGGGCTGTGGTTTCAGCATTCGTCGCAGTTGTTATGTTCACAGCATTGATTCTGCTGTCTGCTGTTGCTCTCCGAAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000381 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006970 GO:0007623 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009605 GO:0009628 GO:0009649 GO:0009651 GO:0009889 GO:0009892 GO:0009914 GO:0009926 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010817 GO:0010866 GO:0010921 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0019216 GO:0019219 GO:0019220 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035303 GO:0040029 GO:0042221 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046890 GO:0048024 GO:0048511 GO:0048519 GO:0050684 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051174 GO:0051179 GO:0051234 GO:0051252 GO:0051336 GO:0051716 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070887 GO:0070918 GO:0071071 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071944 GO:0080090 GO:0090207 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1901703 GO:1903311 GO:1903725 GO:1903730 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPLKIP

Protein Analysis

215

Amino Acids

22.92

Weight (kDa)

7.83

Isoelectric Point (pI)

28.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 52 - 199 3e-45 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24500 AT4G24500 AT4G24500
fragaria_vesca FvH4_5g34870
malus_domestica MD08G1199700.v1.1
prunus_persica Prupe.1G533200_v2.0.a1
pyrus_communis pycom08g17330 pycom15g34700
rosa_chinensis RchiOBHm_Chr7g0235791
rosa_laevigata RLG00000001139
rosa_roxburghii Rroxscaffold_3G00225630
rosa_rugosa Rorug07G0292800
rosa_samantha Rh7AG447700 Rh7BG419900 Rh7CG468200 Rh7DG436700
rosa_wichuraiana Rw7G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
AccB1I GGYRCC 1 cut(s) 191
AciI CCGC 3 cut(s) 458, 468, 564
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 1 cut(s) 327
AcyI GRCGYC 2 cut(s) 192, 230
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 29
AluBI AGCT 5 cut(s) 110, 143, 203, 281, 644
AluI AGCT 5 cut(s) 110, 143, 203, 281, 644
Alw21I GWGCWC 1 cut(s) 171
AlwI GGATC 1 cut(s) 110
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 9 cut(s) 71, 110, 200, 203, 215, 461, 464, 470, 473
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 3 cut(s) 194, 215, 439
AsuHPI GGTGA 1 cut(s) 367
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 9 cut(s) 58, 97, 190, 202, 212, 448, 451, 457, 460
BccI CCATC 1 cut(s) 141
BceAI ACGGC 2 cut(s) 149, 203
BfaI CTAG 4 cut(s) 107, 188, 209, 347
BfmI CTRYAG 1 cut(s) 198
BfoI RGCGCY 3 cut(s) 195, 216, 440
BmiI GGNNCC 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 1 cut(s) 499
BmuI ACTGGG 1 cut(s) 385
BsaHI GRCGYC 2 cut(s) 192, 230
BsaJI CCNNGG 2 cut(s) 225, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 271, 391
Bse3DI GCAATG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 225, 404
BseGI GGATG 2 cut(s) 152, 514
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 271, 391
BseXI GCAGC 9 cut(s) 58, 97, 190, 202, 212, 448, 451, 457, 460
BshFI GGCC 1 cut(s) 329
BshNI GGYRCC 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 171
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 372
BsmI GAATGC 2 cut(s) 33, 581
BsnI GGCC 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 3 cut(s) 27, 115, 127
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 3 cut(s) 458, 468, 564
BspANI GGCC 1 cut(s) 329
BspHI TCATGA 2 cut(s) 96, 130
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 2 cut(s) 271, 391
BssECI CCNNGG 2 cut(s) 225, 404
BssMI GATC 3 cut(s) 27, 115, 127
BssNI GRCGYC 2 cut(s) 192, 230
BssT1I CCWWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 537
Bst6I CTCTTC 1 cut(s) 252
BstACI GRCGYC 2 cut(s) 192, 230
BstC8I GCNNGC 3 cut(s) 239, 522, 554
BstDEI CTNAG 1 cut(s) 182
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 152, 514
BstH2I RGCGCY 3 cut(s) 195, 216, 440
BstHHI GCGC 3 cut(s) 194, 215, 439
BstKTI GATC 3 cut(s) 30, 118, 130
BstMBI GATC 3 cut(s) 27, 115, 127
BstMWI GCNNNNNNNGC 8 cut(s) 200, 212, 415, 464, 467, 470, 473, 587
BstNSI RCATGY 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 9 cut(s) 58, 97, 190, 202, 212, 448, 451, 457, 460
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 2 cut(s) 152, 514
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 239, 522, 554
CciI TCATGA 2 cut(s) 96, 130
CfoI GCGC 3 cut(s) 194, 215, 439
CseI GACGC 1 cut(s) 219
Csp6I GTAC 1 cut(s) 104
CviAII CATG 6 cut(s) 92, 97, 131, 226, 295, 307
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 1 cut(s) 182
DinI GGCGCC 1 cut(s) 193
DpnI GATC 3 cut(s) 29, 117, 129
DpnII GATC 3 cut(s) 27, 115, 127
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 225
EgeI GGCGCC 1 cut(s) 193
EheI GGCGCC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 6 cut(s) 95, 100, 134, 229, 298, 310
FaqI GGGAC 1 cut(s) 372
FatI CATG 6 cut(s) 91, 96, 130, 225, 294, 306
FauI CCCGC 1 cut(s) 557
FokI GGATG 2 cut(s) 159, 521
FspBI CTAG 4 cut(s) 107, 188, 209, 347
GlaI GCGC 3 cut(s) 193, 214, 438
HaeII RGCGCY 3 cut(s) 195, 216, 440
HaeIII GGCC 1 cut(s) 329
HgaI GACGC 1 cut(s) 219
HhaI GCGC 3 cut(s) 194, 215, 439
Hin1I GRCGYC 2 cut(s) 192, 230
Hin1II CATG 6 cut(s) 95, 100, 134, 229, 298, 310
Hin6I GCGC 3 cut(s) 192, 213, 437
HinP1I GCGC 3 cut(s) 192, 213, 437
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 4 cut(s) 14, 44, 244, 613
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 3 cut(s) 484, 540, 603
Hpy188I TCNGA 4 cut(s) 405, 447, 508, 638
Hpy188III TCNNGA 3 cut(s) 57, 97, 131
Hpy8I GTNNAC 3 cut(s) 484, 540, 603
Hpy99I CGWCG 1 cut(s) 590
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4V TGCA 2 cut(s) 200, 512
HpyF10VI GCNNNNNNNGC 8 cut(s) 200, 212, 415, 464, 467, 470, 473, 587
HpyF3I CTNAG 1 cut(s) 182
Hsp92I GRCGYC 2 cut(s) 192, 230
Hsp92II CATG 6 cut(s) 95, 100, 134, 229, 298, 310
HspAI GCGC 3 cut(s) 192, 213, 437
KasI GGCGCC 1 cut(s) 191
Kzo9I GATC 3 cut(s) 27, 115, 127
LmnI GCTCC 2 cut(s) 140, 406
LpnPI CCDG 8 cut(s) 129, 247, 267, 284, 404, 419, 566, 574
Lsp1109I GCAGC 9 cut(s) 58, 97, 190, 202, 212, 448, 451, 457, 460
LweI GCATC 1 cut(s) 499
MaeI CTAG 4 cut(s) 107, 188, 209, 347
MaeIII GTNAC 2 cut(s) 220, 373
MalI GATC 3 cut(s) 29, 117, 129
MboI GATC 3 cut(s) 27, 115, 127
MboII GAAGA 4 cut(s) 163, 239, 247, 259
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 514
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 192
MlyI GAGTC 2 cut(s) 53, 238
MnlI CCTC 5 cut(s) 93, 362, 368, 399, 463
Mox20I TGGCCA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 13
MscI TGGCCA 1 cut(s) 329
MseI TTAA 1 cut(s) 646
MslI CAYNNNNRTG 2 cut(s) 129, 305
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 4 cut(s) 203, 281, 470, 564
Mva1269I GAATGC 2 cut(s) 33, 581
MwoI GCNNNNNNNGC 8 cut(s) 200, 212, 415, 464, 467, 470, 473, 587
NarI GGCGCC 1 cut(s) 192
NcoI CCATGG 1 cut(s) 225
NdeII GATC 3 cut(s) 27, 115, 127
NlaIII CATG 6 cut(s) 95, 100, 134, 229, 298, 310
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 2 cut(s) 220, 373
NspI RCATGY 1 cut(s) 298
PagI TCATGA 2 cut(s) 96, 130
PctI GAATGC 2 cut(s) 33, 581
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 2 cut(s) 14, 613
PleI GAGTC 2 cut(s) 52, 238
PluTI GGCGCC 1 cut(s) 195
PpsI GAGTC 2 cut(s) 52, 238
PspN4I GGNNCC 1 cut(s) 193
PstI CTGCAG 1 cut(s) 202
PvuII CAGCTG 2 cut(s) 203, 281
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 2 cut(s) 129, 305
SaqAI TTAA 1 cut(s) 646
Sau3AI GATC 3 cut(s) 27, 115, 127
SchI GAGTC 2 cut(s) 53, 238
SduI GDGCHC 1 cut(s) 171
SetI ASST 9 cut(s) 69, 112, 145, 160, 205, 283, 348, 455, 646
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 1 cut(s) 198
SfoI GGCGCC 1 cut(s) 193
SmiMI CAYNNNNRTG 2 cut(s) 129, 305
Sse9I AATT 1 cut(s) 514
SsiI CCGC 3 cut(s) 458, 468, 564
SspDI GGCGCC 1 cut(s) 191
SspMI CTAG 4 cut(s) 107, 188, 209, 347
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 537
TaqI TCGA 2 cut(s) 171, 426
TasI AATT 1 cut(s) 514
TauI GCSGC 2 cut(s) 461, 470
TfiI GAWTC 2 cut(s) 14, 613
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 220, 373
TseI GCWGC 9 cut(s) 71, 110, 200, 203, 215, 461, 464, 470, 473
Tsp45I GTSAC 2 cut(s) 220, 373
TspDTI ATGAA 2 cut(s) 166, 295
TspGWI ACGGA 2 cut(s) 240, 374
TspRI CASTG 1 cut(s) 202
XceI RCATGY 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 3 cut(s) 278, 484, 568
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 4 cut(s) 107, 188, 209, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.