Rroxscaffold_3G00225630

M-phase-specific PLK1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014057
Physical Location & Seq
Forward (+)
8917063 .. 8919801
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225630.1

Sequence Viewer

Length: 912 bp
ATGGAGACTTCAGAGAAGAGAAAGGAACGACTAAGAGCAATGCGCCTAGAAGCTGAAGCTTCTCATAATGATACAAGTTCAGATGTACCAGGTTACCTTTCCAATCCGCTGGCTGATGATAATGTGAATGTGGCGCAGGAGGAGCCCTGTGCTCCTTCGAGGTTTGGTTTTTATACAGACCCTATGGCGGGCTTCTCTTCAGACACTAAGCGATGTAAAGTTGGTGATCAGATTGCATCAAATAGTTTCAAACATTCAGATACCGGTGGTCTTCCTATGCCAAGGTTTCCTCCACCTCTTTCAGGAAGACCAAGGAACCCTGAAATGCCTCCTCCACCTCATCAGTTTCAGAGCAATTACTCACCAGACCAGAGAATGTACCAACAAAACTTTGTTCCTCAGAGAAATCCCACAGGAATGGTGAGACCTTTTGCCATGCATCATACAAATCCACCTGAAGTCTGGAATGGAGCTCAAGGTCCGGCCAGTTATAATTTCCCTTCTGATCCCTCAAGTGAAAGCAGTTTTCCTGGCCCTAGATTTAGACCACCAGGTAGCCCCGGATTTAGACCACCGGGAAGCCCCAGATTTGGGCCACCGGGAAGCCCAGGATTTGGACCACCGGGAAGCCCCGGATTTGGGCCACCGGAAGCTCGGGATTTGGACCACCGGGAAGCCCGGGATTTGGACCACCGGGAAGCCCCGGATTTGGGCCACCAGGAAGCCCCAGATTTGGACCACCCGGAAGCCCTGGTTTTGGTTCCAATACTCGGCAAGGTAGGGGACACTGGGCCAGTCATAGTCCAAGCCCTCATTCAGTACGTGGAGGTAGTCATAGTCCTAGATCAAGTTCAGGTAGAGGTAGGGGCACTCTGGGTCAGGATGGCGAGGTGGACGTGGGCGGGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000381 GO:0000398 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0006950 GO:0006970 GO:0007623 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009605 GO:0009628 GO:0009649 GO:0009651 GO:0009889 GO:0009892 GO:0009914 GO:0009926 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010817 GO:0010866 GO:0010921 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016604 GO:0019216 GO:0019219 GO:0019220 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032879 GO:0033036 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035303 GO:0040029 GO:0042221 GO:0042752 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046890 GO:0048024 GO:0048511 GO:0048519 GO:0050684 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051049 GO:0051171 GO:0051174 GO:0051179 GO:0051234 GO:0051252 GO:0051336 GO:0051716 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070887 GO:0070918 GO:0071071 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071944 GO:0080090 GO:0090207 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1901703 GO:1903311 GO:1903725 GO:1903730 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPLKIP

Protein Analysis

303

Amino Acids

33.26

Weight (kDa)

5.6

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014057)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24500 AT4G24500 AT4G24500
fragaria_vesca FvH4_5g34870
malus_domestica MD08G1199700.v1.1
prunus_persica Prupe.1G533200_v2.0.a1
pyrus_communis pycom08g17330 pycom15g34700
rosa_chinensis RchiOBHm_Chr7g0235791
rosa_laevigata RLG00000001139
rosa_roxburghii Rroxscaffold_3G00225630
rosa_rugosa Rorug07G0292800
rosa_samantha Rh7AG447700 Rh7BG419900 Rh7CG468200 Rh7DG436700
rosa_wichuraiana Rw7G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AccB7I CCANNNNNTGG 1 cut(s) 614
AciI CCGC 3 cut(s) 107, 188, 902
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 1 cut(s) 483
AcuI CTGAAG 3 cut(s) 75, 183, 477
AfaI GTAC 3 cut(s) 87, 380, 821
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 1 cut(s) 250
AjiI CACGTC 1 cut(s) 897
AjnI CCWGG 6 cut(s) 88, 529, 550, 607, 717, 750
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
AluBI AGCT 4 cut(s) 53, 59, 473, 653
AluI AGCT 4 cut(s) 53, 59, 473, 653
Alw21I GWGCWC 2 cut(s) 154, 475
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 500
Ama87I CYCGRG 2 cut(s) 654, 678
AoxI GGCC 6 cut(s) 483, 532, 593, 641, 712, 791
AsiGI ACCGGT 1 cut(s) 263
AspLEI GCGC 2 cut(s) 45, 136
AsuHPI GGTGA 3 cut(s) 236, 354, 433
AvaI CYCGRG 2 cut(s) 654, 678
AvaII GGWCC 5 cut(s) 479, 617, 664, 688, 736
BaeGI GKGCMC 1 cut(s) 871
BanII GRGCYC 2 cut(s) 147, 475
BbsI GAAGAC 2 cut(s) 263, 313
Bbv12I GWGCWC 2 cut(s) 154, 475
BccI CCATC 1 cut(s) 877
BciT130I CCWGG 6 cut(s) 90, 531, 552, 609, 719, 752
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 3 cut(s) 47, 537, 842
Bme18I GGWCC 5 cut(s) 479, 617, 664, 688, 736
BmeT110I CYCGRG 2 cut(s) 654, 678
BmgBI CACGTC 1 cut(s) 897
BmiI GGNNCC 3 cut(s) 144, 317, 762
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 2 cut(s) 245, 448
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 2 cut(s) 263, 313
BpuEI CTTGAG 2 cut(s) 459, 496
BsaAI YACGTR 1 cut(s) 823
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 8 cut(s) 281, 311, 559, 607, 631, 678, 702, 750
BsaWI WCCGGW 2 cut(s) 263, 646
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 486, 793, 794
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 6 cut(s) 90, 531, 552, 609, 719, 752
BseDI CCNNGG 8 cut(s) 281, 311, 559, 607, 631, 678, 702, 750
BseGI GGATG 1 cut(s) 888
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 3 cut(s) 486, 793, 794
BseRI GAGGAG 2 cut(s) 155, 321
BseSI GKGCMC 1 cut(s) 871
BshFI GGCC 6 cut(s) 485, 534, 595, 643, 714, 793
BshTI ACCGGT 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 154, 475
BsiHKCI CYCGRG 2 cut(s) 654, 678
BslFI GGGAC 1 cut(s) 797
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 797
BsnI GGCC 6 cut(s) 485, 534, 595, 643, 714, 793
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 2 cut(s) 654, 678
Bsp1286I GDGCHC 4 cut(s) 147, 154, 475, 871
Bsp143I GATC 3 cut(s) 226, 505, 844
BspACI CCGC 3 cut(s) 107, 188, 902
BspANI GGCC 6 cut(s) 485, 534, 595, 643, 714, 793
BspCNI CTCAG 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 144, 317, 762
BspPI GGATC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 486, 793, 794
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 8 cut(s) 281, 311, 559, 607, 631, 678, 702, 750
BssMI GATC 3 cut(s) 226, 505, 844
BssT1I CCWWGG 2 cut(s) 281, 311
Bst2UI CCWGG 6 cut(s) 90, 531, 552, 609, 719, 752
Bst6I CTCTTC 2 cut(s) 11, 202
BstBAI YACGTR 1 cut(s) 823
BstC8I GCNNGC 2 cut(s) 111, 190
BstDEI CTNAG 3 cut(s) 32, 207, 399
BstEII GGTNACC 1 cut(s) 92
BstENI CCTNNNNNAGG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 888
BstHHI GCGC 2 cut(s) 45, 136
BstKTI GATC 3 cut(s) 229, 508, 847
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 3 cut(s) 226, 505, 844
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstNI CCWGG 6 cut(s) 90, 531, 552, 609, 719, 752
BstPI GGTNACC 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 871
BstV2I GAAGAC 2 cut(s) 263, 313
BstXI CCANNNNNNTGG 2 cut(s) 109, 418
BsuRI GGCC 6 cut(s) 485, 534, 595, 643, 714, 793
BtgZI GCGATG 1 cut(s) 226
BtrI CACGTC 1 cut(s) 897
BtsCI GGATG 1 cut(s) 888
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 2 cut(s) 111, 190
CfoI GCGC 2 cut(s) 45, 136
Cfr10I RCCGGY 1 cut(s) 263
Cfr9I CCCGGG 1 cut(s) 678
CsiI ACCWGGT 2 cut(s) 88, 550
Csp6I GTAC 3 cut(s) 86, 379, 820
CspAI ACCGGT 1 cut(s) 263
CviAII CATG 1 cut(s) 436
CviQI GTAC 3 cut(s) 86, 379, 820
DdeI CTNAG 3 cut(s) 32, 207, 399
DpnI GATC 3 cut(s) 228, 507, 846
DpnII GATC 3 cut(s) 226, 505, 844
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 11, 202
EarI CTCTTC 2 cut(s) 11, 202
Ecl136II GAGCTC 1 cut(s) 473
Eco130I CCWWGG 2 cut(s) 281, 311
Eco24I GRGCYC 2 cut(s) 147, 475
Eco31I GGTCTC 1 cut(s) 418
Eco47I GGWCC 5 cut(s) 479, 617, 664, 688, 736
Eco53kI GAGCTC 1 cut(s) 473
Eco57I CTGAAG 3 cut(s) 75, 183, 477
Eco88I CYCGRG 2 cut(s) 654, 678
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 300
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 6 cut(s) 88, 529, 550, 607, 717, 750
EcoT14I CCWWGG 2 cut(s) 281, 311
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 147, 475
ErhI CCWWGG 2 cut(s) 281, 311
FaeI CATG 1 cut(s) 439
FaiI YATR 9 cut(s) 66, 174, 185, 278, 437, 444, 492, 800, 836
FaqI GGGAC 1 cut(s) 797
FatI CATG 1 cut(s) 435
FauI CCCGC 2 cut(s) 181, 895
FbaI TGATCA 1 cut(s) 226
FokI GGATG 1 cut(s) 895
FriOI GRGCYC 2 cut(s) 147, 475
FspBI CTAG 3 cut(s) 47, 537, 842
GlaI GCGC 2 cut(s) 44, 135
HaeIII GGCC 6 cut(s) 485, 534, 595, 643, 714, 793
HhaI GCGC 2 cut(s) 45, 136
Hin1II CATG 1 cut(s) 439
Hin6I GCGC 2 cut(s) 43, 134
HinP1I GCGC 2 cut(s) 43, 134
HindIII AAGCTT 1 cut(s) 57
HphI GGTGA 3 cut(s) 236, 354, 433
Hpy166II GTNNAC 1 cut(s) 894
Hpy188I TCNGA 9 cut(s) 13, 82, 202, 231, 259, 351, 402, 505, 911
Hpy188III TCNNGA 4 cut(s) 303, 463, 656, 880
Hpy8I GTNNAC 1 cut(s) 894
HpyAV CCTTC 2 cut(s) 165, 510
HpyCH4IV ACGT 2 cut(s) 822, 896
HpyCH4V TGCA 2 cut(s) 236, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpyF3I CTNAG 3 cut(s) 32, 207, 399
HpySE526I ACGT 2 cut(s) 822, 896
Hsp92II CATG 1 cut(s) 439
HspAI GCGC 2 cut(s) 43, 134
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 3 cut(s) 226, 505, 844
LmnI GCTCC 3 cut(s) 142, 157, 470
LweI GCATC 2 cut(s) 245, 448
MabI ACCWGGT 2 cut(s) 88, 550
MaeI CTAG 3 cut(s) 47, 537, 842
MaeII ACGT 2 cut(s) 822, 896
MaeIII GTNAC 1 cut(s) 92
MalI GATC 3 cut(s) 228, 507, 846
MboI GATC 3 cut(s) 226, 505, 844
MboII GAAGA 4 cut(s) 28, 189, 263, 318
MhlI GDGCHC 4 cut(s) 147, 154, 475, 871
MluCI AATT 2 cut(s) 355, 493
Mph1103I ATGCAT 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 109
MvaI CCWGG 6 cut(s) 90, 531, 552, 609, 719, 752
MwoI GCNNNNNNNGC 1 cut(s) 142
NdeII GATC 3 cut(s) 226, 505, 844
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 3 cut(s) 144, 317, 762
NmeAIII GCCGAG 1 cut(s) 750
NsiI ATGCAT 1 cut(s) 441
PflMI CCANNNNNTGG 1 cut(s) 614
PinAI ACCGGT 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 823
PsiI TTATAA 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 475
Psp6I CCWGG 6 cut(s) 88, 529, 550, 607, 717, 750
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 6 cut(s) 88, 529, 550, 607, 717, 750
PspN4I GGNNCC 3 cut(s) 144, 317, 762
RsaI GTAC 3 cut(s) 87, 380, 821
RsaNI GTAC 3 cut(s) 86, 379, 820
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 475
Sau3AI GATC 3 cut(s) 226, 505, 844
SduI GDGCHC 4 cut(s) 147, 154, 475, 871
SexAI ACCWGGT 2 cut(s) 88, 550
SfaNI GCATC 2 cut(s) 245, 448
SinI GGWCC 5 cut(s) 479, 617, 664, 688, 736
SmaI CCCGGG 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 474, 511
SmoI CTYRAG 2 cut(s) 474, 511
Sse9I AATT 2 cut(s) 355, 493
SsiI CCGC 3 cut(s) 107, 188, 902
SspMI CTAG 3 cut(s) 47, 537, 842
SstI GAGCTC 1 cut(s) 475
StyI CCWWGG 2 cut(s) 281, 311
TaiI ACGT 2 cut(s) 825, 899
TaqI TCGA 1 cut(s) 158
TasI AATT 2 cut(s) 355, 493
TscAI CASTG 1 cut(s) 793
TspMI CCCGGG 1 cut(s) 678
TspRI CASTG 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 5 cut(s) 479, 617, 664, 688, 736
XagI CCTNNNNNAGG 1 cut(s) 300
XcmI CCANNNNNNNNNTGG 1 cut(s) 459
XmaI CCCGGG 1 cut(s) 678
XspI CTAG 3 cut(s) 47, 537, 842
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.