Prupe.2G258100_v2.0.a1

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013717
Physical Location & Seq
Reverse (-)
26928515 .. 26931841
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G258100.2

Sequence Viewer

Length: 2451 bp
ATGAGCATGGAAATAAATCACTTGATTGGAGAAATCCAACAGGCAGTAGATAATGTGACTCTGGTTTGCCAAGATCCCCACAGCAGCATAGCTTCCGGGGGTGTGTGGCGCCACAGAAGCCCTCTGCGTTCCGCTACATCTCTTCTTTTGATGCAGTTGTCTATAATTGGCGTAGTTTCACAGTTGATCAATCTCTGTCTCAAACCTATGGGACAGTCCACTGTCGTTTCTCAAATTTTTGGTGGTATAATTTTCGGCCCGTCAGTTCTGGGGCATAAAAAGGAAGTCTCACGCATCCTCTTCCCCGTAAAAGGCGCCAATGTGTTCGAAACTACTGCAACATTCGGCCTCATGTTCTTCCTTTTTTCAATAGGAGTGAAGATGAACACGGGAAGAATGTTAAGGCCAGATAGAAGGGCGGTGACCATTGGAATTTCAGTGTTCTTCTTCACCTTAGCGCTCCCTGAATGCCTAGTCTTCGTTATGATGAAGTATCTCGCCATGGACGAAACCCTTAAGATGGCACTCCCATGTATAGCTGGATCACAGTGTATATCAAGTTCCCTTGTTGTTGCTTGTTTACTAGCTGAGCTCAAACTCATGAACACCGAACTGGGCCGCCTGGCCCTCTCTATAACAATGTTTTGTGATGTTCTCGGCATTGCCCTGGTCGCAATTGGTATGGCAATTTTGGATAATAGGACCAGGAATTTATTAATTCCAACGTTTGAACTACTATCAGCACTAGTATTTGTGCTTGGCGTTGCTTATGTGCTCAGACCAATCATACTATGGATGCTGAACCGTATAGAGGAAGGGAAATCTGTCAAGGAGTCATATATTGTCACCATTTTCCTCTTTGTTCTTTTGTGTGGATTTATCAGTGAGCTTGTAAGCCAGCATTTCTTACTTGGACCCCTAGTGCTTGGCTATGCGGTGCCTGAAGGACCGCCTTTGGGAGCGGCTTTGGTGACGAAGGTGGAAATCATGGCTACAGGAATATTCTATCCAACTTACCTTGCCAATAGTGGGCTGAAAACGAACATCTTCAGGATTCATCCTCGGTCTTTGTGGATTGTGGGGGTGGTGGTTATCTTTTCTAGCCTGGTGAAGATAGGAGCTGTAATGTTGTCAGCCAGCTATTTCGATGTGCCTCTGCGCCAAGCTTTTGTGCTTGGCCTCATCCTCAACTCAAAAGGCATCACAGAGCTTGTCATGTTCAACCTGTTTAAGCAAAGCAAGGTTCTGACTGATCAAGAATTTGCGCTGGTCGTGATCTCAGTGGTGCTCATAACAGCAGTGGTAACACCCTTGATAAGATATCTTTATGATCCTTCAAAGCAATACGCAGTGACGAGAAGAAGTACAATCCAGCATTTGAAACGTGAGTCAGAGCTCCGAATCCTAGCATGCATCCACAACCAAGAGAACGTTCCCACATTCATAAACGTCCTGGAAGTATCAAATGCTACAGAACAGAACCCTGTTGCAGTCATAGCCCTCGTCCTCACTGAGCTTGTGGGGCGAACCAACCCTGTTCTTGTATCACACCGTCCCCAAGACACCTTAGACAACAGCTCGTCTGGCCATATTGTCAAAGCCATGAGGCAATACGAGCAATACAATGAAGGGTACGCCACCCTCCAAGCATACACTTCCATCTCGAGTTATGTAACAATGCATGATGATATTTGCCGGTTAGCATTTGAGAAGAGGGTAAACCTTGTGATCATGCCGTTTCACAAGCAATGGGCCATAGATGGCAGCATTGGGTCAGTGAACCGACCCCTCCAGTCCATGAACATCAATGTCCTTGAAAAGGCACCTTGCTCCGTTGGAATTCTCATCGATCGAGGGGTCCTAGGAGGGTCGGTGTCAATGCTAGCCAGCCGGTATATATGCCATGTTGCTGTGATCTTCATTGGTGGTGCAGATGATACAGAGGCACTGGCCTATGGCGCTCGCATGGCAAGGCATCCAAGTGTGGACTTAACAGTTGCACGGTTCCTTCTCTTTGGAGAAGAAAATAGTAAAGACCGGAAGCGGGACTCTGACTTGCTTGAGGAATACCGGGTAGCCAATGGAGACAACGAGCGGTTTGTTGTTGTGGAGGAGGTGGTAAGAGATGGAGCACGGTTGTCTGCTGTTATCAGATCAATGGTGGATTGTTTTGATTTGATGTTGGTAGGGAGGCACCATCAGGACTCTCCACTTCTTTCAGGGCTTGGTGAGTGGAGTGAGTGCCCGGAGCTTGGAATCGTCGGAGACATGCTTGCTTCCCCGGACTTTCATTGTTCAGTTTCTGTATTAGTGTTGCAACAACAGAGAATAGGAGGAAAACCAGTTAGCCGGAATCAACCAATTGACAGAGAACCGCTAATTCATGATGCACCTGAGGAAACAGTAAGAGGATCGTGGACAATTACAGTCAATGAACATGATAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

817

Amino Acids

90.05

Weight (kDa)

6.84

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g25390
malus_domestica MD01G1160100.v1.1 MD07G1228400.v1.1
prunus_persica Prupe.2G258100_v2.0.a1 Prupe.2G258100_v2.0.a1
pyrus_communis pycom01g17650 pycom07g20680
rosa_chinensis RchiOBHm_Chr1g0371341
rosa_laevigata RLG00000026970
rosa_multiflora Rmu_sc0000809.1_g000038
rosa_roxburghii Rroxscaffold_4G00285780
rosa_rugosa Rorug01G0363200 Rorug01G0363300
rosa_samantha Rh1AG373100 Rh1BG336600 Rh1CG350000 Rh1DG367700
rosa_wichuraiana Rw1G032820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 108, 314, 937, 1822, 2193
AccBSI CCGCTC 2 cut(s) 962, 2095
AciI CCGC 9 cut(s) 132, 419, 619, 935, 950, 962, 2044, 2095, 2375
AclI AACGTT 2 cut(s) 725, 1431
AclWI GGATC 4 cut(s) 68, 550, 1325, 2419
AcoI YGGCCR 1 cut(s) 1585
AcsI RAATTY 5 cut(s) 234, 432, 709, 1259, 1839
AcuI CTGAAG 2 cut(s) 963, 1033
AcyI GRCGYC 2 cut(s) 109, 315
AfaI GTAC 2 cut(s) 1366, 1634
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 8 cut(s) 311, 520, 811, 956, 1029, 1819, 2044, 2252
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 6 cut(s) 369, 731, 1222, 1338, 1381, 1817
AhlI ACTAGT 1 cut(s) 745
AjnI CCWGG 5 cut(s) 621, 666, 704, 1104, 1452
AjuI GAANNNNNNNTTGG 2 cut(s) 1416, 1448
Alw21I GWGCWC 5 cut(s) 594, 777, 1290, 1398, 2134
Alw26I GTCTC 4 cut(s) 203, 292, 2079, 2259
AlwI GGATC 4 cut(s) 68, 550, 1325, 2419
AlwNI CAGNNNCTG 1 cut(s) 2303
Ama87I CYCGRG 1 cut(s) 1663
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 9 cut(s) 256, 346, 404, 616, 624, 1177, 1585, 1752, 1950
ApeKI GCWGC 2 cut(s) 84, 1764
ApoI RAATTY 5 cut(s) 234, 432, 709, 1259, 1839
AseI ATTAAT 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 1046
AspA2I CCTAGG 1 cut(s) 1861
AspLEI GCGC 6 cut(s) 111, 317, 460, 1161, 1267, 1961
AspS9I GGNCC 8 cut(s) 257, 616, 625, 702, 914, 947, 1752, 1858
AsuC2I CCSGG 4 cut(s) 97, 2072, 2246, 2282
AsuHPI GGTGA 6 cut(s) 433, 442, 838, 982, 1120, 2240
AsuII TTCGAA 1 cut(s) 327
AsuNHI GCTAGC 1 cut(s) 1882
AvaI CYCGRG 1 cut(s) 1663
AvaII GGWCC 4 cut(s) 702, 914, 947, 1858
AvrII CCTAGG 1 cut(s) 1861
AxyI CCTNAGG 1 cut(s) 2394
BaeGI GKGCMC 1 cut(s) 2246
BalI TGGCCA 1 cut(s) 1587
BanI GGYRCC 5 cut(s) 108, 314, 937, 1822, 2193
BanII GRGCYC 2 cut(s) 594, 1398
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 5 cut(s) 594, 777, 1290, 1398, 2134
BbvI GCAGC 2 cut(s) 96, 1776
BccI CCATC 5 cut(s) 514, 1667, 1754, 2120, 2205
BceAI ACGGC 1 cut(s) 1720
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 5 cut(s) 623, 668, 706, 1106, 1454
BclI TGATCA 3 cut(s) 186, 1252, 1728
BcnI CCSGG 4 cut(s) 97, 2072, 2246, 2282
BcoDI GTCTC 4 cut(s) 203, 292, 2079, 2259
BcuI ACTAGT 1 cut(s) 745
BfaI CTAG 8 cut(s) 473, 584, 746, 920, 1101, 1406, 1862, 1883
BfmI CTRYAG 2 cut(s) 993, 1470
BfoI RGCGCY 4 cut(s) 112, 318, 461, 1962
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 4 cut(s) 85, 619, 963, 1765
BlnI CCTAGG 1 cut(s) 1861
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 4 cut(s) 86, 620, 964, 1766
Bme1390I CCNGG 9 cut(s) 97, 623, 668, 706, 1106, 1454, 2072, 2246, 2282
Bme18I GGWCC 4 cut(s) 702, 914, 947, 1858
BmeT110I CYCGRG 1 cut(s) 1663
BmgT120I GGNCC 8 cut(s) 257, 616, 625, 702, 914, 947, 1752, 1858
BmiI GGNNCC 8 cut(s) 110, 316, 916, 939, 1824, 1859, 2006, 2195
BmrFI CCNGG 9 cut(s) 97, 623, 668, 706, 1106, 1454, 2072, 2246, 2282
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 7 cut(s) 141, 303, 786, 1209, 1422, 1984, 2377
BmtI GCTAGC 1 cut(s) 1886
BmuI ACTGGG 1 cut(s) 623
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 1775
Bpu10I CCTNAGC 1 cut(s) 454
Bpu1102I GCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 2081
BpuMI CCSGG 4 cut(s) 97, 2072, 2246, 2282
Bsa29I ATCGAT 1 cut(s) 1848
BsaHI GRCGYC 2 cut(s) 109, 315
BsaJI CCNNGG 6 cut(s) 96, 501, 666, 1061, 1861, 2280
BsaWI WCCGGW 1 cut(s) 2037
BsaXI ACNNNNNCTCC 2 cut(s) 1857, 1887
Bsc4I CCNNNNNNNGG 8 cut(s) 311, 520, 811, 956, 1029, 1819, 2044, 2252
Bse118I RCCGGY 2 cut(s) 1695, 1890
Bse1I ACTGG 4 cut(s) 618, 1792, 1953, 2342
Bse21I CCTNAGG 1 cut(s) 2394
Bse3DI GCAATG 2 cut(s) 660, 1754
BseBI CCWGG 5 cut(s) 623, 668, 706, 1106, 1454
BseCI ATCGAT 1 cut(s) 1848
BseDI CCNNGG 6 cut(s) 96, 501, 666, 1061, 1861, 2280
BseGI GGATG 6 cut(s) 294, 801, 1057, 1182, 1413, 1975
BseLI CCNNNNNNNGG 8 cut(s) 311, 520, 811, 956, 1029, 1819, 2044, 2252
BseMI GCAATG 2 cut(s) 660, 1754
BseMII CTCAG 5 cut(s) 579, 790, 1293, 1503, 2385
BseNI ACTGG 4 cut(s) 618, 1792, 1953, 2342
BseRI GAGGAG 1 cut(s) 2126
BseSI GKGCMC 1 cut(s) 2246
BseXI GCAGC 2 cut(s) 96, 1776
BsgI GTGCAG 1 cut(s) 1950
Bsh1285I CGRYCG 1 cut(s) 1852
BshFI GGCC 9 cut(s) 258, 348, 406, 618, 626, 1179, 1587, 1754, 1952
BshNI GGYRCC 5 cut(s) 108, 314, 937, 1822, 2193
BshVI ATCGAT 1 cut(s) 1848
BsiEI CGRYCG 1 cut(s) 1852
BsiHKAI GWGCWC 5 cut(s) 594, 777, 1290, 1398, 2134
BsiHKCI CYCGRG 1 cut(s) 1663
BsiSI CCGG 8 cut(s) 96, 1696, 1891, 2038, 2071, 2246, 2282, 2350
BslFI GGGAC 3 cut(s) 225, 1539, 2060
BslI CCNNNNNNNGG 8 cut(s) 311, 520, 811, 956, 1029, 1819, 2044, 2252
BsmAI GTCTC 4 cut(s) 203, 292, 2079, 2259
BsmFI GGGAC 3 cut(s) 225, 1539, 2060
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 9 cut(s) 258, 348, 406, 618, 626, 1179, 1587, 1754, 1952
BsoBI CYCGRG 1 cut(s) 1663
Bsp119I TTCGAA 1 cut(s) 327
Bsp1286I GDGCHC 6 cut(s) 594, 777, 1290, 1398, 2134, 2246
Bsp1720I GCTNAGC 1 cut(s) 588
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 9 cut(s) 132, 419, 619, 935, 950, 962, 2044, 2095, 2375
BspANI GGCC 9 cut(s) 258, 348, 406, 618, 626, 1179, 1587, 1754, 1952
BspCNI CTCAG 5 cut(s) 580, 789, 1292, 1504, 2386
BspDI ATCGAT 1 cut(s) 1848
BspHI TCATGA 2 cut(s) 600, 2383
BspLI GGNNCC 8 cut(s) 110, 316, 916, 939, 1824, 1859, 2006, 2195
BspOI GCTAGC 1 cut(s) 1886
BspPI GGATC 4 cut(s) 68, 550, 1325, 2419
BspT104I TTCGAA 1 cut(s) 327
BspT107I GGYRCC 5 cut(s) 108, 314, 937, 1822, 2193
BspTI CTTAAG 1 cut(s) 515
BsrBI CCGCTC 2 cut(s) 962, 2095
BsrDI GCAATG 2 cut(s) 660, 1754
BsrFI RCCGGY 2 cut(s) 1695, 1890
BsrI ACTGG 4 cut(s) 618, 1792, 1953, 2342
BssAI RCCGGY 2 cut(s) 1695, 1890
BssECI CCNNGG 6 cut(s) 96, 501, 666, 1061, 1861, 2280
BssNI GRCGYC 2 cut(s) 109, 315
BssT1I CCWWGG 2 cut(s) 501, 1861
Bst2UI CCWGG 5 cut(s) 623, 668, 706, 1106, 1454
Bst6I CTCTTC 3 cut(s) 147, 305, 1706
BstACI GRCGYC 2 cut(s) 109, 315
BstAFI CTTAAG 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 7 cut(s) 899, 1138, 1411, 1884, 1888, 1963, 2274
BstDEI CTNAG 7 cut(s) 454, 588, 776, 1279, 1512, 1567, 2394
BstDSI CCRYGG 1 cut(s) 501
BstEII GGTNACC 1 cut(s) 421
BstENI CCTNNNNNAGG 1 cut(s) 1817
BstF5I GGATG 6 cut(s) 294, 801, 1057, 1182, 1413, 1975
BstH2I RGCGCY 4 cut(s) 112, 318, 461, 1962
BstHHI GCGC 6 cut(s) 111, 317, 460, 1161, 1267, 1961
BstMAI GTCTC 4 cut(s) 203, 292, 2079, 2259
BstMCI CGRYCG 1 cut(s) 1852
BstMWI GCNNNNNNNGC 8 cut(s) 117, 671, 1496, 1522, 1584, 1615, 1958, 1967
BstNI CCWGG 5 cut(s) 623, 668, 706, 1106, 1454
BstNSI RCATGY 2 cut(s) 1413, 2272
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 9 cut(s) 95, 621, 666, 704, 1104, 1452, 2070, 2244, 2280
BstSFI CTRYAG 2 cut(s) 993, 1470
BstSLI GKGCMC 1 cut(s) 2246
BstV1I GCAGC 2 cut(s) 96, 1776
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 1848
Bsu36I CCTNAGG 1 cut(s) 2394
BsuRI GGCC 9 cut(s) 258, 348, 406, 618, 626, 1179, 1587, 1754, 1952
BsuTUI ATCGAT 1 cut(s) 1848
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 6 cut(s) 294, 801, 1057, 1182, 1413, 1975
BtsI GCAGTG 2 cut(s) 1305, 1356
Cac8I GCNNGC 7 cut(s) 899, 1138, 1411, 1884, 1888, 1963, 2274
CaiI CAGNNNCTG 1 cut(s) 2303
CciI TCATGA 2 cut(s) 600, 2383
CfoI GCGC 6 cut(s) 111, 317, 460, 1161, 1267, 1961
Cfr10I RCCGGY 2 cut(s) 1695, 1890
Cfr13I GGNCC 8 cut(s) 257, 616, 625, 702, 914, 947, 1752, 1858
ClaI ATCGAT 1 cut(s) 1848
Csp6I GTAC 2 cut(s) 1365, 1633
CviQI GTAC 2 cut(s) 1365, 1633
DdeI CTNAG 7 cut(s) 454, 588, 776, 1279, 1512, 1567, 2394
DinI GGCGCC 2 cut(s) 110, 316
EaeI YGGCCR 1 cut(s) 1585
Eam1104I CTCTTC 3 cut(s) 147, 305, 1706
EarI CTCTTC 3 cut(s) 147, 305, 1706
Ecl136II GAGCTC 2 cut(s) 592, 1396
Eco130I CCWWGG 2 cut(s) 501, 1861
Eco24I GRGCYC 2 cut(s) 594, 1398
Eco32I GATATC 1 cut(s) 1322
Eco47I GGWCC 4 cut(s) 702, 914, 947, 1858
Eco47III AGCGCT 1 cut(s) 459
Eco53kI GAGCTC 2 cut(s) 592, 1396
Eco57I CTGAAG 2 cut(s) 963, 1033
Eco81I CCTNAGG 1 cut(s) 2394
Eco88I CYCGRG 1 cut(s) 1663
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 2 cut(s) 592, 1396
EcoNI CCTNNNNNAGG 1 cut(s) 1817
EcoO109I RGGNCCY 1 cut(s) 1858
EcoO65I GGTNACC 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 1839
EcoRII CCWGG 5 cut(s) 621, 666, 704, 1104, 1452
EcoRV GATATC 1 cut(s) 1322
EcoT14I CCWWGG 2 cut(s) 501, 1861
EcoT22I ATGCAT 2 cut(s) 1415, 1683
EcoT38I GRGCYC 2 cut(s) 594, 1398
EgeI GGCGCC 2 cut(s) 110, 316
EheI GGCGCC 2 cut(s) 110, 316
ErhI CCWWGG 2 cut(s) 501, 1861
FaqI GGGAC 3 cut(s) 225, 1539, 2060
FauI CCCGC 1 cut(s) 2037
FbaI TGATCA 3 cut(s) 186, 1252, 1728
Fnu4HI GCNGC 4 cut(s) 85, 619, 963, 1765
FokI GGATG 6 cut(s) 281, 808, 1044, 1169, 1400, 1962
FriOI GRGCYC 2 cut(s) 594, 1398
Fsp4HI GCNGC 4 cut(s) 85, 619, 963, 1765
FspBI CTAG 8 cut(s) 473, 584, 746, 920, 1101, 1406, 1862, 1883
GlaI GCGC 6 cut(s) 110, 316, 459, 1160, 1266, 1960
GluI GCNGC 4 cut(s) 85, 619, 963, 1765
GsuI CTGGAG 1 cut(s) 1775
HaeII RGCGCY 4 cut(s) 112, 318, 461, 1962
HaeIII GGCC 9 cut(s) 258, 348, 406, 618, 626, 1179, 1587, 1754, 1952
HapII CCGG 8 cut(s) 96, 1696, 1891, 2038, 2071, 2246, 2282, 2350
HhaI GCGC 6 cut(s) 111, 317, 460, 1161, 1267, 1961
Hin1I GRCGYC 2 cut(s) 109, 315
Hin6I GCGC 6 cut(s) 109, 315, 458, 1159, 1265, 1959
HinP1I GCGC 6 cut(s) 109, 315, 458, 1159, 1265, 1959
HindIII AAGCTT 1 cut(s) 1164
HinfI GANTC 9 cut(s) 58, 833, 1054, 1388, 1401, 2048, 2204, 2256, 2353
HpaII CCGG 8 cut(s) 96, 1696, 1891, 2038, 2071, 2246, 2282, 2350
HphI GGTGA 6 cut(s) 433, 442, 838, 982, 1120, 2240
Hpy166II GTNNAC 6 cut(s) 219, 581, 1720, 1780, 1987, 2418
Hpy188I TCNGA 7 cut(s) 779, 1248, 1393, 1400, 2053, 2153, 2264
Hpy188III TCNNGA 7 cut(s) 601, 1051, 1256, 1273, 1663, 2201, 2384
Hpy8I GTNNAC 6 cut(s) 219, 581, 1720, 1780, 1987, 2418
Hpy99I CGWCG 1 cut(s) 2264
HpyAV CCTTC 7 cut(s) 408, 809, 938, 970, 1344, 1622, 2018
HpyCH4IV ACGT 4 cut(s) 725, 1384, 1431, 1449
HpyCH4V TGCA 9 cut(s) 154, 338, 1413, 1490, 1681, 1931, 2000, 2317, 2390
HpyF10VI GCNNNNNNNGC 8 cut(s) 117, 671, 1496, 1522, 1584, 1615, 1958, 1967
HpyF3I CTNAG 7 cut(s) 454, 588, 776, 1279, 1512, 1567, 2394
HpySE526I ACGT 4 cut(s) 725, 1384, 1431, 1449
Hsp92I GRCGYC 2 cut(s) 109, 315
HspAI GCGC 6 cut(s) 109, 315, 458, 1159, 1265, 1959
KasI GGCGCC 2 cut(s) 108, 314
Ksp22I TGATCA 3 cut(s) 186, 1252, 1728
LmnI GCTCC 7 cut(s) 465, 959, 1118, 1401, 1835, 2129, 2248
Lsp1109I GCAGC 2 cut(s) 96, 1776
LweI GCATC 7 cut(s) 141, 303, 786, 1209, 1422, 1984, 2377
MaeI CTAG 8 cut(s) 473, 584, 746, 920, 1101, 1406, 1862, 1883
MaeII ACGT 4 cut(s) 725, 1384, 1431, 1449
MaeIII GTNAC 7 cut(s) 55, 421, 844, 970, 1303, 1351, 1672
MbiI CCGCTC 2 cut(s) 962, 2095
MfeI CAATTG 2 cut(s) 675, 2361
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 6 cut(s) 594, 777, 1290, 1398, 2134, 2246
MlsI TGGCCA 1 cut(s) 1587
MluNI TGGCCA 1 cut(s) 1587
Mly113I GGCGCC 2 cut(s) 109, 315
MlyI GAGTC 5 cut(s) 52, 842, 1397, 2042, 2198
MmeI TCCRAC 5 cut(s) 61, 746, 1034, 1816, 2242
Mox20I TGGCCA 1 cut(s) 1587
Mph1103I ATGCAT 2 cut(s) 1415, 1683
MroXI GAANNNNTTC 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 1587
MseI TTAA 5 cut(s) 401, 516, 716, 1230, 1991
MslI CAYNNNNRTG 2 cut(s) 529, 1980
Msp20I TGGCCA 1 cut(s) 1587
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 8 cut(s) 96, 1696, 1891, 2038, 2071, 2246, 2282, 2350
MspR9I CCNGG 9 cut(s) 97, 623, 668, 706, 1106, 1454, 2072, 2246, 2282
MunI CAATTG 2 cut(s) 675, 2361
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 5 cut(s) 623, 668, 706, 1106, 1454
MwoI GCNNNNNNNGC 8 cut(s) 117, 671, 1496, 1522, 1584, 1615, 1958, 1967
NarI GGCGCC 2 cut(s) 109, 315
NciI CCSGG 4 cut(s) 97, 2072, 2246, 2282
NcoI CCATGG 1 cut(s) 501
NheI GCTAGC 1 cut(s) 1882
NlaIV GGNNCC 8 cut(s) 110, 316, 916, 939, 1824, 1859, 2006, 2195
NmeAIII GCCGAG 1 cut(s) 636
NmuCI GTSAC 5 cut(s) 55, 421, 844, 970, 1351
NsiI ATGCAT 2 cut(s) 1415, 1683
NspI RCATGY 2 cut(s) 1413, 2272
NspV TTCGAA 1 cut(s) 327
PaeI GCATGC 1 cut(s) 1413
PaeR7I CTCGAG 1 cut(s) 1663
PagI TCATGA 2 cut(s) 600, 2383
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PctI GAATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 1046
PfeI GAWTC 4 cut(s) 1054, 1401, 2256, 2353
PfoI TCCNGGA 1 cut(s) 1452
PkrI GCNGC 4 cut(s) 86, 620, 964, 1766
Ple19I CGATCG 1 cut(s) 1852
PleI GAGTC 5 cut(s) 52, 841, 1396, 2042, 2198
PluTI GGCGCC 2 cut(s) 112, 318
PpsI GAGTC 5 cut(s) 52, 841, 1396, 2042, 2198
PpuMI RGGWCCY 1 cut(s) 1858
PshBI ATTAAT 1 cut(s) 716
Psp124BI GAGCTC 2 cut(s) 594, 1398
Psp1406I AACGTT 2 cut(s) 725, 1431
Psp5II RGGWCCY 1 cut(s) 1858
Psp6I CCWGG 5 cut(s) 621, 666, 704, 1104, 1452
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 5 cut(s) 621, 666, 704, 1104, 1452
PspN4I GGNNCC 8 cut(s) 110, 316, 916, 939, 1824, 1859, 2006, 2195
PspPI GGNCC 8 cut(s) 257, 616, 625, 702, 914, 947, 1752, 1858
PspPPI RGGWCCY 1 cut(s) 1858
PsrI GAACNNNNNNTAC 2 cut(s) 544, 576
PstNI CAGNNNCTG 1 cut(s) 2303
PsuI RGATCY 1 cut(s) 73
PvuI CGATCG 1 cut(s) 1852
RsaI GTAC 2 cut(s) 1366, 1634
RsaNI GTAC 2 cut(s) 1365, 1633
RseI CAYNNNNRTG 2 cut(s) 529, 1980
SacI GAGCTC 2 cut(s) 594, 1398
SaqAI TTAA 5 cut(s) 401, 516, 716, 1230, 1991
SatI GCNGC 4 cut(s) 85, 619, 963, 1765
Sau96I GGNCC 8 cut(s) 257, 616, 625, 702, 914, 947, 1752, 1858
SchI GAGTC 5 cut(s) 52, 842, 1397, 2042, 2198
ScrFI CCNGG 9 cut(s) 97, 623, 668, 706, 1106, 1454, 2072, 2246, 2282
SduI GDGCHC 6 cut(s) 594, 777, 1290, 1398, 2134, 2246
SfaNI GCATC 7 cut(s) 141, 303, 786, 1209, 1422, 1984, 2377
SfcI CTRYAG 2 cut(s) 993, 1470
SfoI GGCGCC 2 cut(s) 110, 316
Sfr274I CTCGAG 1 cut(s) 1663
SfuI TTCGAA 1 cut(s) 327
SinI GGWCC 4 cut(s) 702, 914, 947, 1858
SlaI CTCGAG 1 cut(s) 1663
SmiMI CAYNNNNRTG 2 cut(s) 529, 1980
SmlI CTYRAG 3 cut(s) 515, 1663, 2060
SmoI CTYRAG 3 cut(s) 515, 1663, 2060
SpeI ACTAGT 1 cut(s) 745
SphI GCATGC 1 cut(s) 1413
SsiI CCGC 9 cut(s) 132, 419, 619, 935, 950, 962, 2044, 2095, 2375
SspDI GGCGCC 2 cut(s) 108, 314
SspI AATATT 1 cut(s) 1002
SspMI CTAG 8 cut(s) 473, 584, 746, 920, 1101, 1406, 1862, 1883
SstI GAGCTC 2 cut(s) 594, 1398
StyD4I CCNGG 9 cut(s) 95, 621, 666, 704, 1104, 1452, 2070, 2244, 2280
StyI CCWWGG 2 cut(s) 501, 1861
TaiI ACGT 4 cut(s) 728, 1387, 1434, 1452
TaqI TCGA 5 cut(s) 327, 1146, 1664, 1848, 1852
TaqII GACCGA 1 cut(s) 1053
TatI WGTACW 1 cut(s) 1364
TauI GCSGC 2 cut(s) 621, 965
TfiI GAWTC 4 cut(s) 1054, 1401, 2256, 2353
Tru1I TTAA 5 cut(s) 401, 516, 716, 1230, 1991
Tru9I TTAA 5 cut(s) 401, 516, 716, 1230, 1991
TseFI GTSAC 5 cut(s) 55, 421, 844, 970, 1351
TseI GCWGC 2 cut(s) 84, 1764
Tsp45I GTSAC 5 cut(s) 55, 421, 844, 970, 1351
TspGWI ACGGA 1 cut(s) 1822
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 4 cut(s) 702, 914, 947, 1858
VspI ATTAAT 1 cut(s) 716
XagI CCTNNNNNAGG 1 cut(s) 1817
XapI RAATTY 5 cut(s) 234, 432, 709, 1259, 1839
XceI RCATGY 2 cut(s) 1413, 2272
XcmI CCANNNNNNNNNTGG 1 cut(s) 789
XhoI CTCGAG 1 cut(s) 1663
XmaJI CCTAGG 1 cut(s) 1861
XmnI GAANNNNTTC 1 cut(s) 1046
XspI CTAG 8 cut(s) 473, 584, 746, 920, 1101, 1406, 1862, 1883
Zsp2I ATGCAT 2 cut(s) 1415, 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.