pycom07g20680

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013717
Physical Location & Seq
Reverse (-)
22680506 .. 22683177
2672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20680.3

Sequence Viewer

Length: 2349 bp
ATGGGCTTAGAAATGAATGCATTGGTTGGAGACATCGAGCAGGCCATAGACAATGTGACTTTGGTGTGCCAAAATCCGCACAGCAGCATTACTTCCCGCGGACTATTTCGCCACGAGAACCCTCTCGCTTCCTCCACATCTCTTCTTCTGGTGCAGTTATCCATCATCACCCTTGTTTCCCAGTTGATCAATCTCTGCTTCAAACCCCTGGGACAGTCCACCCTCGTTTCTCAGATGTTTGGCGGTGTAATATTCGGGCCATCACTTCTGGGGCACAAAAAGCAAATCGCAGACACCATCTTCCCGATAAAAAGTGCTAATGTGTTTGAAACAATTGCAACATTCGGCCTCATGTTCTTCCTTTTTTCAACGGGAGTGAAGCTGAATGCAGGCAGAACGATAAGGCCTGAACGAAGAGCAGTTTCCATTGCACTTTCGATGTTCTTCTTCACCTTGGCACTCCCCTTATGCCTAACCATGATGATGAGAAAATATGTCGCGATGGACGAGACCCTTAGGTCGGCTCTCCCAACTATAACTGCATCACAGGTTATATCGAGTTCCCCTGTGGTTGGTTGTTTACTAACTGAGCTCAGGCTAATGAACACGGACCTTGGCCGCCTGGCCCTCTCTGTAACAATGTTTTCCGATGTTATGGGCATCAGTATGGTTGGAGTGGCCATGGCGATTTTGGGTAACAAGACGAAAACTGCGTTAGTTCCAGCCTTGGAAATAATAACATTGTTAGCTTTTATTCTTTCGATTATATACTTGTTCAGGCCAGCAATTCTATGGATGCTCAACCGTATAGAGGAAGGGAAATCTGTCAAGGAGTCGTATACCATTACCATTTTCGTGTTTGTTCTTGTGTGTGGATTTCTCAGTGAGCTCATAAGCCAGCATTACTTGCTGGGACCTCTAGTGCTTGGGTACGTCGTGCCTGCAGGACCGCCTTTGGGATCTGCATTGGTGATGAAGTTGGAAATTTTGGCTACAGGATTGTTCTACCCAAGCTACCTTGCCAATAGCGGGCTGAGGACGAACATCTTCAGGGTTAATCCTCGGTCTACGTGGATTATGGGGGTTCTCGTTCTCTTTTCTGCCCTGGTGAAGGTAGGAGCAGTAATGTTACCAGCCACCTATTTTGATGTGCCTACGCATGACGCTTTTGTGCTCGGCATCGTCCTTAACGCCAAAGGCATCACTGAGCTTGTTATGTACAACATTTGGAAGCAAGGCAAGGTTCTGACTGATCAAGAATTCGCGCTGTCCGTGTTCTCAGTGATAGTGATGACAGCAATTGTAACACCTTTGGACAGTGTTCCCACTCTCATAAACGTCCTGGAAGTAACAAATGCTACAGAAGCGAACCCTGTTGCAGTCATAGGCCTCGTCCTCACCGAGCTTGTGGGGCAAACCAACCCTATTCTTGTGGCACACCAACCCCGTGACAACTTTAACAACAACACAGCCATGTCCTGCCATATCGTCAAAGCGCTGAGGCAGTACGGGCAGCATAGCCAAGGGCGTGCCTCCCTCCAAGCATTCACTTCCATCTCACAGTATATAACAATGCATGATGACGTTTGCCGCGTAGCAATGGAGAAGAGGGTTAACCTGGTGATCCTGCCGTTTCACAAGCAATGGGCAGTAGATGGCAACATTGGGACGGTGAACCGAGCTCTCCAGTCCATGAACATGAACGTCATCGAAATGGCGCCTTGCTCTGTTGGAATTCTCGTTGATCGGGGACTGCTAGGAGGGTCGGTATCAGTTCTAACCAGCCAGTATACATTCCATGTTGCTGTGATCTTCATTGGTGGTGCAGACGATGCAGAGGCACTAGCCTATGGCGCTCGCATGGCAAGACATCCGAGCGTGGACTTAACAGTTGCCCGGTTCCTCCTCTTTGGTGATGAAAACAGCAAAGACCGGAAGCGGGAAAGTGACCTGGTTGAGGAATACCGGCTAGCTAATGCAGACAATGAGCGGTTTGTTGTGGTGGAAGAGGTGGTAAGAGACGGAGCAAGATTGTCTGCTGTTATCAAATCAATGGTGGATTGTTTTGATTTGATGTTGGTAGGCATGCACCACCAGGACTCCCCGCTTCTTTCGGGGCTCGGTGAGTGGAGTGAGTGCCCGGAGCTCGGAATCATCGGGGATATGCTTGCTTCCCCGGACTTCCAGTGCTCACTTTCTGTGCTAGTGCTGCAGCAGCAGAGAATAGGAGGGAAACCGGTTAATCGGAATCAAGCAGTTGACAAAGAACCACTAATTCACGATGCACCACCTGAGGAAACACTCAGAGGATCCTGGACGATTACAGTTAGCGAAAATGGTAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

783

Amino Acids

85.41

Weight (kDa)

6.23

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g25390
malus_domestica MD01G1160100.v1.1 MD07G1228400.v1.1
prunus_persica Prupe.2G258100_v2.0.a1 Prupe.2G258100_v2.0.a1
pyrus_communis pycom01g17650 pycom07g20680
rosa_chinensis RchiOBHm_Chr1g0371341
rosa_laevigata RLG00000026970
rosa_multiflora Rmu_sc0000809.1_g000038
rosa_roxburghii Rroxscaffold_4G00285780
rosa_rugosa Rorug01G0363200 Rorug01G0363300
rosa_samantha Rh1AG373100 Rh1BG336600 Rh1CG350000 Rh1DG367700
rosa_wichuraiana Rw1G032820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 1717
AccBSI CCGCTC 1 cut(s) 1990
AccI GTMKAC 3 cut(s) 839, 1067, 1790
AccII CGCG 4 cut(s) 99, 500, 1265, 1593
AclWI GGATC 4 cut(s) 967, 1618, 2304, 2317
AcoI YGGCCR 2 cut(s) 616, 678
AcsI RAATTY 3 cut(s) 984, 1259, 1734
AcuI CTGAAG 1 cut(s) 1033
AcyI GRCGYC 1 cut(s) 1718
AfaI GTAC 3 cut(s) 932, 1220, 1508
AfeI AGCGCT 1 cut(s) 1497
AfiI CCNNNNNNNGG 9 cut(s) 520, 572, 811, 956, 1029, 1111, 1939, 1957, 2147
AgeI ACCGGT 1 cut(s) 2236
AgsI TTSAA 3 cut(s) 202, 329, 369
AjnI CCWGG 8 cut(s) 207, 621, 1104, 1341, 1617, 1950, 2094, 2312
Alw21I GWGCWC 6 cut(s) 594, 891, 1176, 1684, 2148, 2192
Alw26I GTCTC 3 cut(s) 24, 503, 2013
AlwI GGATC 4 cut(s) 967, 1618, 2304, 2317
Aor51HI AGCGCT 1 cut(s) 1497
AoxI GGCC 9 cut(s) 42, 257, 346, 404, 616, 624, 678, 779, 1387
ApeKI GCWGC 5 cut(s) 84, 1513, 2209, 2212, 2215
ApoI RAATTY 3 cut(s) 984, 1259, 1734
AsiGI ACCGGT 1 cut(s) 2236
Asp700I GAANNNNTTC 1 cut(s) 1046
AspLEI GCGC 4 cut(s) 1267, 1498, 1720, 1856
AspS9I GGNCC 5 cut(s) 257, 610, 625, 914, 947
AsuC2I CCSGG 3 cut(s) 1897, 2141, 2177
AsuHPI GGTGA 9 cut(s) 160, 442, 982, 1120, 1390, 1633, 1684, 1925, 2135
AsuNHI GCTAGC 1 cut(s) 1969
AvaII GGWCC 3 cut(s) 610, 914, 947
AxyI CCTNAGG 2 cut(s) 515, 2292
BaeGI GKGCMC 2 cut(s) 276, 2141
BalI TGGCCA 1 cut(s) 680
BamHI GGATCC 1 cut(s) 2309
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 5 cut(s) 594, 891, 1684, 2121, 2148
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 6 cut(s) 594, 891, 1176, 1684, 2148, 2192
BbvCI CCTCAGC 2 cut(s) 1034, 1499
BbvI GCAGC 5 cut(s) 96, 1525, 2196, 2224, 2227
BccI CCATC 6 cut(s) 170, 268, 305, 496, 1562, 1649
BceAI ACGGC 1 cut(s) 1615
BciT130I CCWGG 8 cut(s) 209, 623, 1106, 1343, 1619, 1952, 2096, 2314
BclI TGATCA 2 cut(s) 186, 1252
BcnI CCSGG 3 cut(s) 1897, 2141, 2177
BcoDI GTCTC 3 cut(s) 24, 503, 2013
BfaI CTAG 5 cut(s) 920, 1757, 1844, 1970, 2204
BfmI CTRYAG 4 cut(s) 942, 993, 1359, 2210
BfoI RGCGCY 3 cut(s) 1499, 1721, 1857
BisI GCNGC 7 cut(s) 85, 619, 1514, 1591, 2210, 2213, 2216
BlsI GCNGC 7 cut(s) 86, 620, 1515, 1592, 2211, 2214, 2217
Bme18I GGWCC 3 cut(s) 610, 914, 947
BmgT120I GGNCC 5 cut(s) 257, 610, 625, 914, 947
BmiI GGNNCC 4 cut(s) 915, 1719, 1901, 2311
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 7 cut(s) 551, 669, 786, 1188, 1209, 1822, 2272
BmtI GCTAGC 1 cut(s) 1973
BmuI ACTGGG 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 1670
Bpu10I CCTNAGC 3 cut(s) 593, 1034, 1499
BpuMI CCSGG 3 cut(s) 1897, 2141, 2177
BsaAI YACGTR 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 1718
BsaI GGTCTC 1 cut(s) 503
BsaWI WCCGGW 2 cut(s) 1932, 2236
BsaXI ACNNNNNCTCC 4 cut(s) 1752, 1782, 2084, 2114
Bsc4I CCNNNNNNNGG 9 cut(s) 520, 572, 811, 956, 1029, 1111, 1939, 1957, 2147
Bse118I RCCGGY 2 cut(s) 1965, 2236
Bse1I ACTGG 4 cut(s) 181, 1687, 1786, 2185
Bse21I CCTNAGG 2 cut(s) 515, 2292
Bse3DI GCAATG 3 cut(s) 426, 1605, 1649
BseBI CCWGG 8 cut(s) 209, 623, 1106, 1343, 1619, 1952, 2096, 2314
BseGI GGATG 2 cut(s) 801, 1870
BseLI CCNNNNNNNGG 9 cut(s) 520, 572, 811, 956, 1029, 1111, 1939, 1957, 2147
BseMI GCAATG 3 cut(s) 426, 1605, 1649
BseNI ACTGG 4 cut(s) 181, 1687, 1786, 2185
BseRI GAGGAG 1 cut(s) 1895
BseSI GKGCMC 2 cut(s) 276, 2141
BseXI GCAGC 5 cut(s) 96, 1525, 2196, 2224, 2227
BseYI CCCAGC 1 cut(s) 910
BsgI GTGCAG 2 cut(s) 173, 1845
Bsh1236I CGCG 4 cut(s) 99, 500, 1265, 1593
BshFI GGCC 9 cut(s) 44, 259, 348, 406, 618, 626, 680, 781, 1389
BshNI GGYRCC 1 cut(s) 1717
BshTI ACCGGT 1 cut(s) 2236
BsiHKAI GWGCWC 6 cut(s) 594, 891, 1176, 1684, 2148, 2192
BsiSI CCGG 6 cut(s) 1897, 1933, 1966, 2141, 2177, 2237
BslFI GGGAC 4 cut(s) 225, 927, 1681, 1764
BslI CCNNNNNNNGG 9 cut(s) 520, 572, 811, 956, 1029, 1111, 1939, 1957, 2147
BsmAI GTCTC 3 cut(s) 24, 503, 2013
BsmBI CGTCTC 1 cut(s) 2013
BsmFI GGGAC 4 cut(s) 225, 927, 1681, 1764
BsmI GAATGC 3 cut(s) 22, 391, 1544
BsnI GGCC 9 cut(s) 44, 259, 348, 406, 618, 626, 680, 781, 1389
Bso31I GGTCTC 1 cut(s) 503
Bsp1286I GDGCHC 9 cut(s) 276, 594, 891, 1176, 1684, 2121, 2141, 2148, 2192
Bsp1407I TGTACA 1 cut(s) 1218
Bsp143I GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
Bsp19I CCATGG 1 cut(s) 681
Bsp68I TCGCGA 1 cut(s) 500
BspANI GGCC 9 cut(s) 44, 259, 348, 406, 618, 626, 680, 781, 1389
BspFNI CGCG 4 cut(s) 99, 500, 1265, 1593
BspLI GGNNCC 4 cut(s) 915, 1719, 1901, 2311
BspMAI CTGCAG 2 cut(s) 946, 2214
BspOI GCTAGC 1 cut(s) 1973
BspPI GGATC 4 cut(s) 967, 1618, 2304, 2317
BspQI GCTCTTC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 1717
BspTNI GGTCTC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 1990
BsrDI GCAATG 3 cut(s) 426, 1605, 1649
BsrFI RCCGGY 2 cut(s) 1965, 2236
BsrGI TGTACA 1 cut(s) 1218
BsrI ACTGG 4 cut(s) 181, 1687, 1786, 2185
BssAI RCCGGY 2 cut(s) 1965, 2236
BssMI GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
BssNAI GTATAC 2 cut(s) 840, 1791
BssNI GRCGYC 1 cut(s) 1718
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 5 cut(s) 453, 613, 681, 726, 1522
Bst1107I GTATAC 2 cut(s) 840, 1791
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 8 cut(s) 209, 623, 1106, 1343, 1619, 1952, 2096, 2314
Bst4CI ACNGT 7 cut(s) 216, 806, 1319, 1563, 1672, 1891, 2326
Bst6I CTCTTC 4 cut(s) 147, 409, 1601, 2001
BstACI GRCGYC 1 cut(s) 1718
BstAPI GCANNNNNTGC 2 cut(s) 907, 1832
BstAUI TGTACA 1 cut(s) 1218
BstBAI YACGTR 1 cut(s) 1071
BstDSI CCRYGG 2 cut(s) 97, 681
BstENI CCTNNNNNAGG 2 cut(s) 1109, 1955
BstF5I GGATG 2 cut(s) 801, 1870
BstFNI CGCG 4 cut(s) 99, 500, 1265, 1593
BstH2I RGCGCY 3 cut(s) 1499, 1721, 1857
BstHHI GCGC 4 cut(s) 1267, 1498, 1720, 1856
BstKTI GATC 7 cut(s) 189, 962, 1255, 1626, 1747, 1812, 2312
BstMAI GTCTC 3 cut(s) 24, 503, 2013
BstMBI GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
BstNI CCWGG 8 cut(s) 209, 623, 1106, 1343, 1619, 1952, 2096, 2314
BstNSI RCATGY 1 cut(s) 2089
BstSFI CTRYAG 4 cut(s) 942, 993, 1359, 2210
BstSLI GKGCMC 2 cut(s) 276, 2141
BstUI CGCG 4 cut(s) 99, 500, 1265, 1593
BstV1I GCAGC 5 cut(s) 96, 1525, 2196, 2224, 2227
BstX2I RGATCY 2 cut(s) 959, 2309
BstYI RGATCY 2 cut(s) 959, 2309
BstZ17I GTATAC 2 cut(s) 840, 1791
Bsu36I CCTNAGG 2 cut(s) 515, 2292
BsuRI GGCC 9 cut(s) 44, 259, 348, 406, 618, 626, 680, 781, 1389
BtgI CCRYGG 2 cut(s) 97, 681
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 2 cut(s) 801, 1870
BtsIMutI CAGTG 5 cut(s) 889, 1203, 1287, 1324, 2192
BtuMI TCGCGA 1 cut(s) 500
CfoI GCGC 4 cut(s) 1267, 1498, 1720, 1856
Cfr10I RCCGGY 2 cut(s) 1965, 2236
Cfr13I GGNCC 5 cut(s) 257, 610, 625, 914, 947
Cfr42I CCGCGG 1 cut(s) 100
CseI GACGC 1 cut(s) 1172
CsiI ACCWGGT 2 cut(s) 1617, 1950
Csp6I GTAC 3 cut(s) 931, 1219, 1507
CspAI ACCGGT 1 cut(s) 2236
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviQI GTAC 3 cut(s) 931, 1219, 1507
DinI GGCGCC 1 cut(s) 1719
DpnI GATC 7 cut(s) 188, 961, 1254, 1625, 1746, 1811, 2311
DpnII GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
DrdI GACNNNNNNGTC 1 cut(s) 517
DseDI GACNNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 2 cut(s) 616, 678
Eam1104I CTCTTC 4 cut(s) 147, 409, 1601, 2001
EarI CTCTTC 4 cut(s) 147, 409, 1601, 2001
Ecl136II GAGCTC 4 cut(s) 592, 889, 1682, 2146
Eco130I CCWWGG 5 cut(s) 453, 613, 681, 726, 1522
Eco147I AGGCCT 2 cut(s) 406, 1389
Eco24I GRGCYC 5 cut(s) 594, 891, 1684, 2121, 2148
Eco31I GGTCTC 1 cut(s) 503
Eco47I GGWCC 3 cut(s) 610, 914, 947
Eco47III AGCGCT 1 cut(s) 1497
Eco53kI GAGCTC 4 cut(s) 592, 889, 1682, 2146
Eco57I CTGAAG 1 cut(s) 1033
Eco81I CCTNAGG 2 cut(s) 515, 2292
EcoICRI GAGCTC 4 cut(s) 592, 889, 1682, 2146
EcoNI CCTNNNNNAGG 2 cut(s) 1109, 1955
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 2 cut(s) 1259, 1734
EcoRII CCWGG 8 cut(s) 207, 621, 1104, 1341, 1617, 1950, 2094, 2312
EcoT14I CCWWGG 5 cut(s) 453, 613, 681, 726, 1522
EcoT22I ATGCAT 2 cut(s) 22, 1578
EcoT38I GRGCYC 5 cut(s) 594, 891, 1684, 2121, 2148
EgeI GGCGCC 1 cut(s) 1719
EheI GGCGCC 1 cut(s) 1719
ErhI CCWWGG 5 cut(s) 453, 613, 681, 726, 1522
Esp3I CGTCTC 1 cut(s) 2013
FaqI GGGAC 4 cut(s) 225, 927, 1681, 1764
FauI CCCGC 4 cut(s) 104, 1022, 1932, 2112
FbaI TGATCA 2 cut(s) 186, 1252
FblI GTMKAC 3 cut(s) 839, 1067, 1790
Fnu4HI GCNGC 7 cut(s) 85, 619, 1514, 1591, 2210, 2213, 2216
FokI GGATG 2 cut(s) 808, 1857
FriOI GRGCYC 5 cut(s) 594, 891, 1684, 2121, 2148
Fsp4HI GCNGC 7 cut(s) 85, 619, 1514, 1591, 2210, 2213, 2216
FspBI CTAG 5 cut(s) 920, 1757, 1844, 1970, 2204
GlaI GCGC 4 cut(s) 1266, 1497, 1719, 1855
GluI GCNGC 7 cut(s) 85, 619, 1514, 1591, 2210, 2213, 2216
GsaI CCCAGC 1 cut(s) 914
GsuI CTGGAG 1 cut(s) 1670
HaeII RGCGCY 3 cut(s) 1499, 1721, 1857
HaeIII GGCC 9 cut(s) 44, 259, 348, 406, 618, 626, 680, 781, 1389
HapII CCGG 6 cut(s) 1897, 1933, 1966, 2141, 2177, 2237
HgaI GACGC 1 cut(s) 1172
HhaI GCGC 4 cut(s) 1267, 1498, 1720, 1856
Hin1I GRCGYC 1 cut(s) 1718
Hin6I GCGC 4 cut(s) 1265, 1496, 1718, 1854
HinP1I GCGC 4 cut(s) 1265, 1496, 1718, 1854
HincII GTYRAC 2 cut(s) 1615, 2260
HindII GTYRAC 2 cut(s) 1615, 2260
HinfI GANTC 4 cut(s) 833, 2099, 2151, 2248
HpaI GTTAAC 1 cut(s) 1615
HpaII CCGG 6 cut(s) 1897, 1933, 1966, 2141, 2177, 2237
HphI GGTGA 9 cut(s) 160, 442, 982, 1120, 1390, 1633, 1684, 1925, 2135
Hpy166II GTNNAC 9 cut(s) 219, 581, 840, 1068, 1615, 1675, 1791, 1882, 2260
Hpy188I TCNGA 7 cut(s) 234, 649, 1248, 1875, 2150, 2247, 2306
Hpy188III TCNNGA 4 cut(s) 304, 499, 1256, 2279
Hpy8I GTNNAC 9 cut(s) 219, 581, 840, 1068, 1615, 1675, 1791, 1882, 2260
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 2 cut(s) 809, 1105
HpyCH4III ACNGT 7 cut(s) 216, 806, 1319, 1563, 1672, 1891, 2326
HpyCH4IV ACGT 5 cut(s) 933, 1070, 1338, 1584, 1704
HpySE526I ACGT 5 cut(s) 933, 1070, 1338, 1584, 1704
Hsp92I GRCGYC 1 cut(s) 1718
HspAI GCGC 4 cut(s) 1265, 1496, 1718, 1854
KasI GGCGCC 1 cut(s) 1717
Ksp22I TGATCA 2 cut(s) 186, 1252
KspAI GTTAAC 1 cut(s) 1615
KspI CCGCGG 1 cut(s) 100
Kzo9I GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 3 cut(s) 1118, 2024, 2143
Lsp1109I GCAGC 5 cut(s) 96, 1525, 2196, 2224, 2227
LweI GCATC 7 cut(s) 551, 669, 786, 1188, 1209, 1822, 2272
MabI ACCWGGT 2 cut(s) 1617, 1950
MaeI CTAG 5 cut(s) 920, 1757, 1844, 1970, 2204
MaeII ACGT 5 cut(s) 933, 1070, 1338, 1584, 1704
MaeIII GTNAC 8 cut(s) 55, 634, 695, 1128, 1303, 1348, 1448, 1946
MalI GATC 7 cut(s) 188, 961, 1254, 1625, 1746, 1811, 2311
MbiI CCGCTC 1 cut(s) 1990
MboI GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
MfeI CAATTG 2 cut(s) 333, 1299
MflI RGATCY 2 cut(s) 959, 2309
MhlI GDGCHC 9 cut(s) 276, 594, 891, 1176, 1684, 2121, 2141, 2148, 2192
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 7 cut(s) 333, 786, 984, 1259, 1299, 1734, 2274
MluNI TGGCCA 1 cut(s) 680
Mly113I GGCGCC 1 cut(s) 1718
MlyI GAGTC 2 cut(s) 842, 2093
MmeI TCCRAC 4 cut(s) 7, 652, 960, 1711
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 2 cut(s) 22, 1578
MroXI GAANNNNTTC 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 680
MseI TTAA 6 cut(s) 1056, 1188, 1458, 1614, 1886, 2241
MslI CAYNNNNRTG 6 cut(s) 482, 665, 854, 1472, 1697, 1712
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 6 cut(s) 1897, 1933, 1966, 2141, 2177, 2237
MunI CAATTG 2 cut(s) 333, 1299
Mva1269I GAATGC 3 cut(s) 22, 391, 1544
MvaI CCWGG 8 cut(s) 209, 623, 1106, 1343, 1619, 1952, 2096, 2314
MvnI CGCG 4 cut(s) 99, 500, 1265, 1593
NarI GGCGCC 1 cut(s) 1718
NciI CCSGG 3 cut(s) 1897, 2141, 2177
NcoI CCATGG 1 cut(s) 681
NdeII GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
NheI GCTAGC 1 cut(s) 1969
NlaIV GGNNCC 4 cut(s) 915, 1719, 1901, 2311
NmeAIII GCCGAG 1 cut(s) 1155
NmuCI GTSAC 3 cut(s) 55, 1448, 1946
NruI TCGCGA 1 cut(s) 500
NsiI ATGCAT 2 cut(s) 22, 1578
NspI RCATGY 1 cut(s) 2089
PaeI GCATGC 1 cut(s) 2089
PasI CCCWGGG 1 cut(s) 208
PceI AGGCCT 2 cut(s) 406, 1389
PciSI GCTCTTC 1 cut(s) 409
PcsI WCGNNNNNNNCGW 5 cut(s) 504, 710, 1269, 1398, 1590
PctI GAATGC 3 cut(s) 22, 391, 1544
PdmI GAANNNNTTC 1 cut(s) 1046
PfeI GAWTC 2 cut(s) 2151, 2248
PfoI TCCNGGA 2 cut(s) 1341, 2312
PinAI ACCGGT 1 cut(s) 2236
PkrI GCNGC 7 cut(s) 86, 620, 1515, 1592, 2211, 2214, 2217
PleI GAGTC 2 cut(s) 841, 2093
PluTI GGCGCC 1 cut(s) 1721
PpsI GAGTC 2 cut(s) 841, 2093
Ppu21I YACGTR 1 cut(s) 1071
PpuMI RGGWCCY 1 cut(s) 914
Psp124BI GAGCTC 4 cut(s) 594, 891, 1684, 2148
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 8 cut(s) 207, 621, 1104, 1341, 1617, 1950, 2094, 2312
PspFI CCCAGC 1 cut(s) 910
PspGI CCWGG 8 cut(s) 207, 621, 1104, 1341, 1617, 1950, 2094, 2312
PspN4I GGNNCC 4 cut(s) 915, 1719, 1901, 2311
PspPI GGNCC 5 cut(s) 257, 610, 625, 914, 947
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 2 cut(s) 946, 2214
PsuI RGATCY 2 cut(s) 959, 2309
RruI TCGCGA 1 cut(s) 500
RsaI GTAC 3 cut(s) 932, 1220, 1508
RsaNI GTAC 3 cut(s) 931, 1219, 1507
RseI CAYNNNNRTG 6 cut(s) 482, 665, 854, 1472, 1697, 1712
SacI GAGCTC 4 cut(s) 594, 891, 1684, 2148
SacII CCGCGG 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 6 cut(s) 1056, 1188, 1458, 1614, 1886, 2241
SatI GCNGC 7 cut(s) 85, 619, 1514, 1591, 2210, 2213, 2216
Sau3AI GATC 7 cut(s) 186, 959, 1252, 1623, 1744, 1809, 2309
Sau96I GGNCC 5 cut(s) 257, 610, 625, 914, 947
SbfI CCTGCAGG 1 cut(s) 946
SchI GAGTC 2 cut(s) 842, 2093
SdaI CCTGCAGG 1 cut(s) 946
SduI GDGCHC 9 cut(s) 276, 594, 891, 1176, 1684, 2121, 2141, 2148, 2192
SexAI ACCWGGT 2 cut(s) 1617, 1950
SfaNI GCATC 7 cut(s) 551, 669, 786, 1188, 1209, 1822, 2272
SfcI CTRYAG 4 cut(s) 942, 993, 1359, 2210
SfoI GGCGCC 1 cut(s) 1719
Sfr303I CCGCGG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 100
SinI GGWCC 3 cut(s) 610, 914, 947
SmiMI CAYNNNNRTG 6 cut(s) 482, 665, 854, 1472, 1697, 1712
SphI GCATGC 1 cut(s) 2089
Sse8387I CCTGCAGG 1 cut(s) 946
Sse9I AATT 7 cut(s) 333, 786, 984, 1259, 1299, 1734, 2274
SseBI AGGCCT 2 cut(s) 406, 1389
SspDI GGCGCC 1 cut(s) 1717
SspI AATATT 1 cut(s) 252
SspMI CTAG 5 cut(s) 920, 1757, 1844, 1970, 2204
SstI GAGCTC 4 cut(s) 594, 891, 1684, 2148
StuI AGGCCT 2 cut(s) 406, 1389
StyI CCWWGG 5 cut(s) 453, 613, 681, 726, 1522
TaaI ACNGT 7 cut(s) 216, 806, 1319, 1563, 1672, 1891, 2326
TaiI ACGT 5 cut(s) 936, 1073, 1341, 1587, 1707
TaqI TCGA 5 cut(s) 36, 437, 557, 761, 1710
TaqII GACCGA 1 cut(s) 1053
TasI AATT 7 cut(s) 333, 786, 984, 1259, 1299, 1734, 2274
TatI WGTACW 1 cut(s) 1218
TauI GCSGC 2 cut(s) 621, 1593
TfiI GAWTC 2 cut(s) 2151, 2248
Tru1I TTAA 6 cut(s) 1056, 1188, 1458, 1614, 1886, 2241
Tru9I TTAA 6 cut(s) 1056, 1188, 1458, 1614, 1886, 2241
TscAI CASTG 5 cut(s) 889, 1210, 1287, 1324, 2192
TseFI GTSAC 3 cut(s) 55, 1448, 1946
TseI GCWGC 5 cut(s) 84, 1513, 2209, 2212, 2215
Tsp45I GTSAC 3 cut(s) 55, 1448, 1946
TspDTI ATGAA 7 cut(s) 29, 617, 989, 1709, 1715, 1804, 1932
TspGWI ACGGA 3 cut(s) 623, 1261, 2037
TspRI CASTG 5 cut(s) 889, 1210, 1287, 1324, 2192
VpaK11BI GGWCC 3 cut(s) 610, 914, 947
XagI CCTNNNNNAGG 2 cut(s) 1109, 1955
XapI RAATTY 3 cut(s) 984, 1259, 1734
XceI RCATGY 1 cut(s) 2089
XcmI CCANNNNNNNNNTGG 2 cut(s) 688, 789
XmiI GTMKAC 3 cut(s) 839, 1067, 1790
XmnI GAANNNNTTC 1 cut(s) 1046
XspI CTAG 5 cut(s) 920, 1757, 1844, 1970, 2204
Zsp2I ATGCAT 2 cut(s) 22, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.