Rroxscaffold_4G00285780

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013717
Physical Location & Seq
Forward (+)
6968879 .. 6971169
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285780.1

Sequence Viewer

Length: 2094 bp
ATGGATGCTGGAGGAATTGTGCGTCCCCAACGGAAGTGCTTGGCCATTGGAACATCATTGTTCCTTTGCACATTGATACTCCCCGAGGGACTAACCTTCCTCTTGATACGCTATGTCCCCAAATTGGACAGGTCGCTTCGGAACTCACTCCCCTTCTTATCTGCTACGCAAGCTTTGACAAGCTCCCCTGTTATTGGTTCTCTACTTACCGAGCTCAAGATTTTGAACACCGACATTGGCCGCACGGCGGTCTCAACGGCAATATTTTGTGATGCTGTTGGCATAACGATGAGTGCGATTGCTATTGCAATAAGTGATGGGAGGACCAAAAATACAATAATACCATGGCTCGCAGTACTACAAGCAGCGGCTCTTGTGTTGGTCATTCTCCTTGTGGCCAGGCCAATCATGATATGGATACGAAACCAGGCACTGGAAAGAAAATCTGTCAGGGAGTCGCATATTTTCATCATTTTCGTTTTTGTGCTTGTGTTTGCATTTCTTAGTGAGATCTCAAGCCAGCATTACGTATTGGGGCCGCTAGCGTTTGGCTTTATAATACCTGCCGGACCGCCTTTAGGCTCAGCTGTGGTGGCCAGGGTGGATACTCTTGCTACAGGACTATTCTACCCAACATACCTTGCCGTCACGGGGCTGAAAACAAACATCTACACGATTGAACTTCGGTCTTCGTGGGTTCTCGGGGTTATTTTTCTCTTCGCTAGCGTGGTCAAGATTGGAGCAGTAATGTTGCCAGCCTGCTATTATGAACAGACTATACGTGAAGCTTTTGTGCTCGGGCTTATTCTCAACGCAAAAGGCATCACTGAGCTTATTATGTTCAACATTTGGAAGCAAACCAAGCTGCTGAGCGATCAAGAATATGCCCTGGCTGTGATCTATGTAGTAGCGGTAACAGCTGTTGTGACACCCTTGATAAAACTTCTCTATGATCCCTCAAAGCAATTCTCAGCTACCAAAAGAAGTACAATCCAGCATTTGAAGCGCGAGACAGAGCTCCGAATCATGGCCTGCATCCACTCTGAGGATAACATTCCCACAATGGTAAACGTCCTAGAAGTATCAAATGCAACAGAAGAGAACCCTGTTGCAGTCATTGCCCTCATCCTCGTCGAGCTTGTGGGACGAATCACCCCTGTTCTCATAGCACATCACCCCAATGACAATCTAAACACCAGCGTTAACAGCAGCACATCCAACTCCAACAAAATTATCAAAGGCTTGAGTCAGTATGCCGAGTACAATGAACGGCGCGCCAGCCTCCAACCGTTCACTTCCATCTCACATTATCCAACAATGCACGAAGACATTGTAAGGATAGCATTTGAGAAGAAGGCACACATTGTGATCCTGCCGTTTCACAAGCATTATGCAATAGATGGCACCATTGGGTTAGTGAACAGAGCTCACCAGTCCCTGAACATCAATGTACTTGAAAAGGCACCTTGCTCAGTTGGAATCCTCATTGATCGGGGAATCCTAGGCGGTTCGGTATCAGTTCTTAACAGCCAGTTTATATTCCATGTTGCTGTGATCTTCATAGGTGGTGTAGACGATGCAGAGGCACTGGCCTATGGCTCTCGCATGGCAAGTCATCCAAATGTGGACTTAACAGTTGCACGGTTTCTTCTCTTTGGAGAAGAAAATTGTAAAGAAAGGAAGCAGGACAGTGACTTGCTTGAAGAATACAGGCGAGCTAATGCAGGAAATGAGCGGTTTGTTGTGGTGGAAGAAGTGGTAAAAGATGGAGCGAAGCTGTCTTCTGTCATCAGTTCAATGGTAGATTGCTTTGATTTGATGTTGGTGGGGATGCATCATCAGGATTCCCAGCTTCTTTCGGGGATTGGTAACTGGAGTGAGTGCCCAGAGCTTGGTATCATTGGCGATATGCTTTCTTCGGCGGATTTTCAGTGCTCACTTTCGGTACTAGTCGTGCAACAACAGAGAATATCAGGAAAACCAATTAGTCGGACTGCAAAACCTGTTGATAAAGAACCGCTAATTCATGATGTCCCGGTAGAACAAATGAGAGGATCATGGACAATTACAGTGAATGAAAATGATGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

697

Amino Acids

76.5

Weight (kDa)

6.51

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 21 - 312 6.2e-29 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 372 - 505 1.5e-15 Plant cation/H(+) antiporter domain
CHX17_C PF23259 515 - 655 8.8e-16 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g25390
malus_domestica MD01G1160100.v1.1 MD07G1228400.v1.1
prunus_persica Prupe.2G258100_v2.0.a1 Prupe.2G258100_v2.0.a1
pyrus_communis pycom01g17650 pycom07g20680
rosa_chinensis RchiOBHm_Chr1g0371341
rosa_laevigata RLG00000026970
rosa_multiflora Rmu_sc0000809.1_g000038
rosa_roxburghii Rroxscaffold_4G00285780
rosa_rugosa Rorug01G0363200 Rorug01G0363300
rosa_samantha Rh1AG373100 Rh1BG336600 Rh1CG350000 Rh1DG367700
rosa_wichuraiana Rw1G032820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 2 cut(s) 1403, 1462
AccB7I CCANNNNNTGG 2 cut(s) 433, 1892
AccBSI CCGCTC 1 cut(s) 1735
AccI GTMKAC 1 cut(s) 1572
AccII CGCG 2 cut(s) 1008, 1275
AclWI GGATC 3 cut(s) 947, 1363, 2062
AcoI YGGCCR 4 cut(s) 42, 238, 396, 594
AdeI CACNNNGTG 1 cut(s) 1366
AfaI GTAC 5 cut(s) 357, 988, 1262, 1452, 1947
AfiI CCNNNNNNNGG 9 cut(s) 124, 194, 247, 433, 578, 651, 1027, 1045, 1892
AgsI TTSAA 7 cut(s) 226, 680, 844, 1003, 1457, 1703, 1797
AhlI ACTAGT 1 cut(s) 1948
AjnI CCWGG 4 cut(s) 398, 426, 596, 888
Alw21I GWGCWC 5 cut(s) 216, 798, 1020, 1429, 1937
Alw26I GTCTC 2 cut(s) 256, 1004
AlwI GGATC 3 cut(s) 947, 1363, 2062
AlwNI CAGNNNCTG 2 cut(s) 433, 1438
Ama87I CYCGRG 3 cut(s) 83, 701, 797
AoxI GGCC 8 cut(s) 42, 238, 396, 401, 536, 594, 1029, 1590
ApeKI GCWGC 3 cut(s) 365, 865, 1209
AscI GGCGCGCC 1 cut(s) 1273
AspA2I CCTAGG 1 cut(s) 1501
AspLEI GCGC 3 cut(s) 1008, 1275, 1277
AspS9I GGNCC 3 cut(s) 324, 536, 569
AsuC2I CCSGG 1 cut(s) 2036
AsuHPI GGTGA 3 cut(s) 1144, 1166, 1421
AsuNHI GCTAGC 2 cut(s) 541, 722
AvaI CYCGRG 3 cut(s) 83, 701, 797
AvaII GGWCC 2 cut(s) 324, 569
AvrII CCTAGG 1 cut(s) 1501
BaeGI GKGCMC 1 cut(s) 1886
BalI TGGCCA 3 cut(s) 44, 398, 596
BanI GGYRCC 2 cut(s) 1403, 1462
BanII GRGCYC 3 cut(s) 216, 1020, 1429
BbsI GAAGAC 3 cut(s) 681, 1332, 1773
Bbv12I GWGCWC 5 cut(s) 216, 798, 1020, 1429, 1937
BbvI GCAGC 3 cut(s) 377, 852, 1221
BccI CCATC 5 cut(s) 311, 1307, 1394, 1760, 2078
BceAI ACGGC 5 cut(s) 261, 273, 629, 1286, 1360
BciT130I CCWGG 4 cut(s) 400, 428, 598, 890
BciVI GTATCC 2 cut(s) 411, 598
BcnI CCSGG 1 cut(s) 2036
BcoDI GTCTC 2 cut(s) 256, 1004
BcuI ACTAGT 1 cut(s) 1948
BfaI CTAG 6 cut(s) 542, 723, 1076, 1502, 1949, 2092
BfmI CTRYAG 1 cut(s) 615
BfuAI ACCTGC 1 cut(s) 571
BfuI GTATCC 2 cut(s) 411, 598
BglII AGATCT 1 cut(s) 510
BisI GCNGC 6 cut(s) 241, 366, 369, 539, 866, 1210
BlnI CCTAGG 1 cut(s) 1501
BlpI GCTNAGC 2 cut(s) 583, 869
BlsI GCNGC 6 cut(s) 242, 367, 370, 540, 867, 1211
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 5 cut(s) 400, 428, 598, 890, 2036
Bme18I GGWCC 2 cut(s) 324, 569
BmeT110I CYCGRG 3 cut(s) 83, 701, 797
BmgT120I GGNCC 3 cut(s) 324, 536, 569
BmiI GGNNCC 3 cut(s) 537, 1405, 1464
BmrFI CCNGG 5 cut(s) 400, 428, 598, 890, 2036
BmsI GCATC 6 cut(s) 262, 831, 1044, 1567, 1821, 1843
BmtI GCTAGC 2 cut(s) 545, 726
BpiI GAAGAC 3 cut(s) 681, 1332, 1773
BpmI CTGGAG 2 cut(s) 30, 1894
Bpu1102I GCTNAGC 2 cut(s) 583, 869
BpuEI CTTGAG 3 cut(s) 200, 499, 1264
BpuMI CCSGG 1 cut(s) 2036
BsaAI YACGTR 2 cut(s) 529, 782
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 5 cut(s) 84, 344, 597, 888, 1501
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 9 cut(s) 124, 194, 247, 433, 578, 651, 1027, 1045, 1892
Bse1I ACTGG 5 cut(s) 438, 1432, 1531, 1593, 1877
Bse3DI GCAATG 1 cut(s) 1116
BseBI CCWGG 4 cut(s) 400, 428, 598, 890
BseDI CCNNGG 5 cut(s) 84, 344, 597, 888, 1501
BseGI GGATG 6 cut(s) 10, 1035, 1125, 1214, 1615, 1836
BseLI CCNNNNNNNGG 9 cut(s) 124, 194, 247, 433, 578, 651, 1027, 1045, 1892
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 6 cut(s) 597, 819, 860, 984, 1035, 1485
BseNI ACTGG 5 cut(s) 438, 1432, 1531, 1593, 1877
BsePI GCGCGC 1 cut(s) 1273
BseSI GKGCMC 1 cut(s) 1886
BseXI GCAGC 3 cut(s) 377, 852, 1221
BseYI CCCAGC 1 cut(s) 1848
Bsh1236I CGCG 2 cut(s) 1008, 1275
BshFI GGCC 8 cut(s) 44, 240, 398, 403, 538, 596, 1031, 1592
BshNI GGYRCC 2 cut(s) 1403, 1462
BsiHKAI GWGCWC 5 cut(s) 216, 798, 1020, 1429, 1937
BsiHKCI CYCGRG 3 cut(s) 83, 701, 797
BsiSI CCGG 2 cut(s) 567, 2036
BslFI GGGAC 6 cut(s) 9, 101, 102, 1158, 1420, 2018
BslI CCNNNNNNNGG 9 cut(s) 124, 194, 247, 433, 578, 651, 1027, 1045, 1892
BsmAI GTCTC 2 cut(s) 256, 1004
BsmFI GGGAC 6 cut(s) 9, 101, 102, 1158, 1420, 2018
BsnI GGCC 8 cut(s) 44, 240, 398, 403, 538, 596, 1031, 1592
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 3 cut(s) 83, 701, 797
Bsp1286I GDGCHC 6 cut(s) 216, 798, 1020, 1429, 1886, 1937
Bsp143I GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
Bsp1720I GCTNAGC 2 cut(s) 583, 869
Bsp19I CCATGG 1 cut(s) 344
BspANI GGCC 8 cut(s) 44, 240, 398, 403, 538, 596, 1031, 1592
BspCNI CTCAG 6 cut(s) 596, 820, 861, 983, 1036, 1484
BspFNI CGCG 2 cut(s) 1008, 1275
BspHI TCATGA 2 cut(s) 408, 2026
BspLI GGNNCC 3 cut(s) 537, 1405, 1464
BspMI ACCTGC 1 cut(s) 571
BspOI GCTAGC 2 cut(s) 545, 726
BspPI GGATC 3 cut(s) 947, 1363, 2062
BspT107I GGYRCC 2 cut(s) 1403, 1462
BspTNI GGTCTC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 1735
BsrDI GCAATG 1 cut(s) 1116
BsrI ACTGG 5 cut(s) 438, 1432, 1531, 1593, 1877
BssECI CCNNGG 5 cut(s) 84, 344, 597, 888, 1501
BssHII GCGCGC 1 cut(s) 1273
BssMI GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
BssT1I CCWWGG 2 cut(s) 344, 1501
Bst2UI CCWGG 4 cut(s) 400, 428, 598, 890
Bst4CI ACNGT 5 cut(s) 1290, 1636, 1644, 1691, 2071
Bst6I CTCTTC 2 cut(s) 722, 1092
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstBAI YACGTR 2 cut(s) 529, 782
BstDEI CTNAG 7 cut(s) 503, 583, 828, 869, 970, 1044, 1471
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 6 cut(s) 10, 1035, 1125, 1214, 1615, 1836
BstFNI CGCG 2 cut(s) 1008, 1275
BstHHI GCGC 3 cut(s) 1008, 1275, 1277
BstKTI GATC 8 cut(s) 513, 877, 900, 955, 1371, 1492, 1557, 2057
BstMAI GTCTC 2 cut(s) 256, 1004
BstMBI GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
BstMWI GCNNNNNNNGC 7 cut(s) 170, 593, 862, 917, 1003, 1118, 1206
BstNI CCWGG 4 cut(s) 400, 428, 598, 890
BstSCI CCNGG 5 cut(s) 398, 426, 596, 888, 2034
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 1 cut(s) 1886
BstSNI TACGTA 1 cut(s) 529
BstUI CGCG 2 cut(s) 1008, 1275
BstV1I GCAGC 3 cut(s) 377, 852, 1221
BstV2I GAAGAC 3 cut(s) 681, 1332, 1773
BstX2I RGATCY 1 cut(s) 510
BstYI RGATCY 1 cut(s) 510
BsuI GTATCC 2 cut(s) 411, 598
BsuRI GGCC 8 cut(s) 44, 240, 398, 403, 538, 596, 1031, 1592
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 6 cut(s) 10, 1035, 1125, 1214, 1615, 1836
BtsIMutI CAGTG 6 cut(s) 431, 825, 1586, 1696, 1937, 2076
BveI ACCTGC 1 cut(s) 571
CaiI CAGNNNCTG 2 cut(s) 433, 1438
CciI TCATGA 2 cut(s) 408, 2026
CfoI GCGC 3 cut(s) 1008, 1275, 1277
Cfr13I GGNCC 3 cut(s) 324, 536, 569
CpoI CGGWCCG 1 cut(s) 569
CseI GACGC 1 cut(s) 11
Csp6I GTAC 5 cut(s) 356, 987, 1261, 1451, 1946
CspI CGGWCCG 1 cut(s) 569
CviAII CATG 7 cut(s) 345, 409, 1027, 1544, 1606, 2027, 2058
CviQI GTAC 5 cut(s) 356, 987, 1261, 1451, 1946
DdeI CTNAG 7 cut(s) 503, 583, 828, 869, 970, 1044, 1471
DpnI GATC 8 cut(s) 512, 876, 899, 954, 1370, 1491, 1556, 2056
DpnII GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
DraIII CACNNNGTG 1 cut(s) 1366
EaeI YGGCCR 4 cut(s) 42, 238, 396, 594
Eam1104I CTCTTC 2 cut(s) 722, 1092
EarI CTCTTC 2 cut(s) 722, 1092
EciI GGCGGA 1 cut(s) 1937
Ecl136II GAGCTC 3 cut(s) 214, 1018, 1427
Eco105I TACGTA 1 cut(s) 529
Eco130I CCWWGG 2 cut(s) 344, 1501
Eco24I GRGCYC 3 cut(s) 216, 1020, 1429
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 324, 569
Eco53kI GAGCTC 3 cut(s) 214, 1018, 1427
Eco88I CYCGRG 3 cut(s) 83, 701, 797
EcoICRI GAGCTC 3 cut(s) 214, 1018, 1427
EcoRII CCWGG 4 cut(s) 398, 426, 596, 888
EcoT14I CCWWGG 2 cut(s) 344, 1501
EcoT22I ATGCAT 1 cut(s) 1836
EcoT38I GRGCYC 3 cut(s) 216, 1020, 1429
ErhI CCWWGG 2 cut(s) 344, 1501
FaeI CATG 7 cut(s) 348, 412, 1030, 1547, 1609, 2030, 2061
FaqI GGGAC 6 cut(s) 9, 101, 102, 1158, 1420, 2018
FatI CATG 7 cut(s) 344, 408, 1026, 1543, 1605, 2026, 2057
FblI GTMKAC 1 cut(s) 1572
Fnu4HI GCNGC 6 cut(s) 241, 366, 369, 539, 866, 1210
FokI GGATG 6 cut(s) 17, 1022, 1112, 1201, 1602, 1843
FriOI GRGCYC 3 cut(s) 216, 1020, 1429
Fsp4HI GCNGC 6 cut(s) 241, 366, 369, 539, 866, 1210
FspBI CTAG 6 cut(s) 542, 723, 1076, 1502, 1949, 2092
GlaI GCGC 3 cut(s) 1007, 1274, 1276
GluI GCNGC 6 cut(s) 241, 366, 369, 539, 866, 1210
GsaI CCCAGC 1 cut(s) 1852
GsuI CTGGAG 2 cut(s) 30, 1894
HaeIII GGCC 8 cut(s) 44, 240, 398, 403, 538, 596, 1031, 1592
HapII CCGG 2 cut(s) 567, 2036
HgaI GACGC 1 cut(s) 11
HhaI GCGC 3 cut(s) 1008, 1275, 1277
Hin1II CATG 7 cut(s) 348, 412, 1030, 1547, 1609, 2030, 2061
Hin6I GCGC 3 cut(s) 1006, 1273, 1275
HinP1I GCGC 3 cut(s) 1006, 1273, 1275
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HindIII AAGCTT 2 cut(s) 171, 786
HinfI GANTC 7 cut(s) 455, 1023, 1149, 1246, 1479, 1497, 1844
HpaI GTTAAC 1 cut(s) 1204
HpaII CCGG 2 cut(s) 567, 2036
HphI GGTGA 3 cut(s) 1144, 1166, 1421
Hpy166II GTNNAC 6 cut(s) 1069, 1204, 1293, 1420, 1573, 1627
Hpy188I TCNGA 4 cut(s) 141, 1022, 1045, 1992
Hpy188III TCNNGA 8 cut(s) 103, 217, 409, 733, 878, 1841, 1974, 2027
Hpy8I GTNNAC 6 cut(s) 1069, 1204, 1293, 1420, 1573, 1627
Hpy99I CGWCG 1 cut(s) 1136
HpyAV CCTTC 3 cut(s) 106, 163, 1348
HpyCH4III ACNGT 5 cut(s) 1290, 1636, 1644, 1691, 2071
HpyCH4IV ACGT 3 cut(s) 528, 781, 1071
HpyF10VI GCNNNNNNNGC 7 cut(s) 170, 593, 862, 917, 1003, 1118, 1206
HpyF3I CTNAG 7 cut(s) 503, 583, 828, 869, 970, 1044, 1471
HpySE526I ACGT 3 cut(s) 528, 781, 1071
Hsp92II CATG 7 cut(s) 348, 412, 1030, 1547, 1609, 2030, 2061
HspAI GCGC 3 cut(s) 1006, 1273, 1275
KspAI GTTAAC 1 cut(s) 1204
Kzo9I GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
LmnI GCTCC 4 cut(s) 188, 740, 1023, 1769
Lsp1109I GCAGC 3 cut(s) 377, 852, 1221
LweI GCATC 6 cut(s) 262, 831, 1044, 1567, 1821, 1843
MaeI CTAG 6 cut(s) 542, 723, 1076, 1502, 1949, 2092
MaeII ACGT 3 cut(s) 528, 781, 1071
MaeIII GTNAC 5 cut(s) 646, 913, 925, 1691, 1868
MalI GATC 8 cut(s) 512, 876, 899, 954, 1370, 1491, 1556, 2056
MbiI CCGCTC 1 cut(s) 1735
MboI GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
MflI RGATCY 1 cut(s) 510
MhlI GDGCHC 6 cut(s) 216, 798, 1020, 1429, 1886, 1937
MlsI TGGCCA 3 cut(s) 44, 398, 596
MluCI AATT 8 cut(s) 15, 122, 965, 1230, 1666, 1983, 2022, 2064
MluNI TGGCCA 3 cut(s) 44, 398, 596
MlyI GAGTC 2 cut(s) 464, 1255
MmeI TCCRAC 6 cut(s) 1242, 1248, 1309, 1337, 1456, 1970
Mox20I TGGCCA 3 cut(s) 44, 398, 596
Mph1103I ATGCAT 1 cut(s) 1836
MscI TGGCCA 3 cut(s) 44, 398, 596
MseI TTAA 3 cut(s) 1203, 1524, 1631
MslI CAYNNNNRTG 3 cut(s) 287, 1179, 1620
Msp20I TGGCCA 3 cut(s) 44, 398, 596
MspA1I CMGCKG 3 cut(s) 368, 587, 920
MspI CCGG 2 cut(s) 567, 2036
MspR9I CCNGG 5 cut(s) 400, 428, 598, 890, 2036
MvaI CCWGG 4 cut(s) 400, 428, 598, 890
MvnI CGCG 2 cut(s) 1008, 1275
MwoI GCNNNNNNNGC 7 cut(s) 170, 593, 862, 917, 1003, 1118, 1206
NciI CCSGG 1 cut(s) 2036
NcoI CCATGG 1 cut(s) 344
NdeII GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
NheI GCTAGC 2 cut(s) 541, 722
NlaIII CATG 7 cut(s) 348, 412, 1030, 1547, 1609, 2030, 2061
NlaIV GGNNCC 3 cut(s) 537, 1405, 1464
NmeAIII GCCGAG 1 cut(s) 1282
NmuCI GTSAC 3 cut(s) 646, 925, 1691
NsiI ATGCAT 1 cut(s) 1836
PagI TCATGA 2 cut(s) 408, 2026
PalAI GGCGCGCC 1 cut(s) 1273
PauI GCGCGC 1 cut(s) 1273
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 5 cut(s) 1023, 1149, 1479, 1497, 1844
PflMI CCANNNNNTGG 2 cut(s) 433, 1892
PkrI GCNGC 6 cut(s) 242, 367, 370, 540, 867, 1211
PleI GAGTC 2 cut(s) 463, 1254
PpsI GAGTC 2 cut(s) 463, 1254
Ppu21I YACGTR 2 cut(s) 529, 782
PsiI TTATAA 1 cut(s) 557
Psp124BI GAGCTC 3 cut(s) 216, 1020, 1429
Psp6I CCWGG 4 cut(s) 398, 426, 596, 888
PspFI CCCAGC 1 cut(s) 1848
PspGI CCWGG 4 cut(s) 398, 426, 596, 888
PspN4I GGNNCC 3 cut(s) 537, 1405, 1464
PspPI GGNCC 3 cut(s) 324, 536, 569
PsrI GAACNNNNNNTAC 2 cut(s) 762, 794
PstNI CAGNNNCTG 2 cut(s) 433, 1438
PsuI RGATCY 1 cut(s) 510
PteI GCGCGC 1 cut(s) 1273
PvuII CAGCTG 2 cut(s) 587, 920
RsaI GTAC 5 cut(s) 357, 988, 1262, 1452, 1947
RsaNI GTAC 5 cut(s) 356, 987, 1261, 1451, 1946
RseI CAYNNNNRTG 3 cut(s) 287, 1179, 1620
Rsr2I CGGWCCG 1 cut(s) 569
RsrII CGGWCCG 1 cut(s) 569
SacI GAGCTC 3 cut(s) 216, 1020, 1429
SaqAI TTAA 3 cut(s) 1203, 1524, 1631
SatI GCNGC 6 cut(s) 241, 366, 369, 539, 866, 1210
Sau3AI GATC 8 cut(s) 510, 874, 897, 952, 1368, 1489, 1554, 2054
Sau96I GGNCC 3 cut(s) 324, 536, 569
ScaI AGTACT 1 cut(s) 357
SchI GAGTC 2 cut(s) 464, 1255
ScrFI CCNGG 5 cut(s) 400, 428, 598, 890, 2036
SduI GDGCHC 6 cut(s) 216, 798, 1020, 1429, 1886, 1937
SfaNI GCATC 6 cut(s) 262, 831, 1044, 1567, 1821, 1843
SfcI CTRYAG 1 cut(s) 615
SgsI GGCGCGCC 1 cut(s) 1273
SinI GGWCC 2 cut(s) 324, 569
SmiMI CAYNNNNRTG 3 cut(s) 287, 1179, 1620
SmlI CTYRAG 3 cut(s) 215, 514, 1243
SmoI CTYRAG 3 cut(s) 215, 514, 1243
SnaBI TACGTA 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 1948
Sse9I AATT 8 cut(s) 15, 122, 965, 1230, 1666, 1983, 2022, 2064
SspI AATATT 1 cut(s) 264
SspMI CTAG 6 cut(s) 542, 723, 1076, 1502, 1949, 2092
SstI GAGCTC 3 cut(s) 216, 1020, 1429
StyD4I CCNGG 5 cut(s) 398, 426, 596, 888, 2034
StyI CCWWGG 2 cut(s) 344, 1501
TaaI ACNGT 5 cut(s) 1290, 1636, 1644, 1691, 2071
TaiI ACGT 3 cut(s) 531, 784, 1074
TaqI TCGA 1 cut(s) 1134
TaqII GACCGA 1 cut(s) 675
TasI AATT 8 cut(s) 15, 122, 965, 1230, 1666, 1983, 2022, 2064
TatI WGTACW 4 cut(s) 355, 986, 1260, 1450
TauI GCSGC 3 cut(s) 243, 371, 541
TfiI GAWTC 5 cut(s) 1023, 1149, 1479, 1497, 1844
Tru1I TTAA 3 cut(s) 1203, 1524, 1631
Tru9I TTAA 3 cut(s) 1203, 1524, 1631
TscAI CASTG 6 cut(s) 438, 832, 1593, 1696, 1937, 2076
TseFI GTSAC 3 cut(s) 646, 925, 1691
TseI GCWGC 3 cut(s) 365, 865, 1209
Tsp45I GTSAC 3 cut(s) 646, 925, 1691
TspDTI ATGAA 6 cut(s) 457, 783, 1281, 1549, 2015, 2091
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 6 cut(s) 438, 832, 1593, 1696, 1937, 2076
Van91I CCANNNNNTGG 2 cut(s) 433, 1892
VpaK11BI GGWCC 2 cut(s) 324, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmaJI CCTAGG 1 cut(s) 1501
XmiI GTMKAC 1 cut(s) 1572
XspI CTAG 6 cut(s) 542, 723, 1076, 1502, 1949, 2092
ZrmI AGTACT 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 1836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.