pycom01g17650

cation H( ) antiporter 15-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013717
Physical Location & Seq
Reverse (-)
16853380 .. 16854537
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17650.1

Sequence Viewer

Length: 1158 bp
ATGACAGCAATTGTAACACCTTTGATAAAGTTTCTTGATGATCCTTCAAACAAATACACAGCACTTAAAAGAAGTACTATCCAGCATTTGAAGCGCGAGTCAGAGCTCCAAATCCTGGCCTGTATCGACAACCACGACAATGTTCCCACTGTCATAAACGTACTGGAGGTATCAAATGCCACAGAACAGAACCCTGTTGCAGTCATAGGACTCATCCTCACCGAGCTTGTGGGGCGAACCAACCCTATTCTTGTGGCACACCAACCCCATGACAACTTGGACAACAATACAGCCATGTCCTGCCATATTGTCAAAGCACTGAGGCAGTACGAGCAGCATAACGAAGGGTGCGTATCCCTCCAAGCATTCACTTCCATCTCACAGTATATAACAATGCATGAGGATGTTTGCCGGGTAGCATTAGAGAATAGGGTTAACCTGGTGATCCTGCCATTTCACAAGCAATGGGCAGTAGATGGCAGCATTGGGGCAGTGAACCGAGCTCTCCGGTCCATGAACAAGAATGTCCTCGAAATGGCGCCTTGCTCGGTCGGAATTCTCATTGACCGGGGACTGCTAGCAGGGACGGTGTCAGTTCTAACCAGCCAGTATATATTCCATGTTGCTGTGATCTTCATTGGTGGTGCAGACGATGCAGAGGCACTAGCCTATGGTGCTCGCATGGCAAGACATCGGAGCGTGGATTTAACAGTTGCCCGGTTGCTTCTCTTTGGTGATGAAAACAGTAAAGAACGGAAGCGGGACACTGACCTGGTTGATGAATACCGGCTAGCTAATGCAGACAATGAGCGGTTTGTTGTCGTGGAAGAGCTGGTAAGAGATGGAGCAAGATTGTCTGCTGTTATCAAATCAATGGTGGATTGTTTTGATTTGATATTGGTAGGCATGCACCATCAGGACTCCCCACTTCTTTCGGGGCTTGGTGAGTGGAGTGAGTGCCCGGAGCTTGGAATCATCGGGGATATGCTTGCTTCCTCGGATTTTCATTGCTCAGTTTCTGTACTAGTGTTGCAGCAACAGAGAATAGGAGGGAAACCAGTTAGTCGGAATCAACCAGTTGACAGAGAACCACTAATTCAGGATGCACCACCTGAGGAAACAGTAAGAGGATCCTGGACAATTACAGTTAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

386

Amino Acids

42.55

Weight (kDa)

5.32

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHX17_2nd PF23256 99 - 193 1.1e-16 Plant cation/H(+) antiporter domain
CHX17_C PF23259 207 - 347 6.6e-17 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013717)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g25390
malus_domestica MD01G1160100.v1.1 MD07G1228400.v1.1
prunus_persica Prupe.2G258100_v2.0.a1 Prupe.2G258100_v2.0.a1
pyrus_communis pycom01g17650 pycom07g20680
rosa_chinensis RchiOBHm_Chr1g0371341
rosa_laevigata RLG00000026970
rosa_multiflora Rmu_sc0000809.1_g000038
rosa_roxburghii Rroxscaffold_4G00285780
rosa_rugosa Rorug01G0363200 Rorug01G0363300
rosa_samantha Rh1AG373100 Rh1BG336600 Rh1CG350000 Rh1DG367700
rosa_wichuraiana Rw1G032820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 538
AccB7I CCANNNNNTGG 1 cut(s) 115
AccBSI CCGCTC 1 cut(s) 811
AccII CGCG 1 cut(s) 96
AciI CCGC 2 cut(s) 760, 811
AclWI GGATC 4 cut(s) 35, 439, 1125, 1138
AcsI RAATTY 1 cut(s) 555
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 4 cut(s) 76, 162, 329, 1023
AfiI CCNNNNNNNGG 3 cut(s) 115, 535, 968
AgsI TTSAA 2 cut(s) 48, 91
AhlI ACTAGT 1 cut(s) 1024
AjnI CCWGG 4 cut(s) 114, 438, 771, 1133
AluBI AGCT 6 cut(s) 106, 226, 503, 794, 832, 967
AluI AGCT 6 cut(s) 106, 226, 503, 794, 832, 967
Alw21I GWGCWC 3 cut(s) 108, 505, 679
AlwI GGATC 4 cut(s) 35, 439, 1125, 1138
AlwNI CAGNNNCTG 1 cut(s) 1019
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 334, 480, 1033
ApoI RAATTY 1 cut(s) 555
AspLEI GCGC 2 cut(s) 96, 541
AspS9I GGNCC 1 cut(s) 510
AsuC2I CCSGG 4 cut(s) 413, 569, 718, 962
AsuHPI GGTGA 4 cut(s) 211, 454, 746, 956
AsuNHI GCTAGC 2 cut(s) 577, 790
AvaII GGWCC 1 cut(s) 510
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 1 cut(s) 962
BamHI GGATCC 1 cut(s) 1130
BanI GGYRCC 1 cut(s) 538
BanII GRGCYC 2 cut(s) 108, 505
Bbv12I GWGCWC 3 cut(s) 108, 505, 679
BbvI GCAGC 3 cut(s) 346, 492, 1045
BccI CCATC 4 cut(s) 383, 470, 836, 921
BciT130I CCWGG 4 cut(s) 116, 440, 773, 1135
BciVI GTATCC 1 cut(s) 364
BcnI CCSGG 4 cut(s) 413, 569, 718, 962
BcuI ACTAGT 1 cut(s) 1024
BfaI CTAG 4 cut(s) 578, 665, 791, 1025
BfoI RGCGCY 1 cut(s) 542
BfuI GTATCC 1 cut(s) 364
BisI GCNGC 3 cut(s) 335, 481, 1034
BlsI GCNGC 3 cut(s) 336, 482, 1035
BmcAI AGTACT 1 cut(s) 76
Bme1390I CCNGG 8 cut(s) 116, 413, 440, 569, 718, 773, 962, 1135
Bme18I GGWCC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 540, 1132
BmrFI CCNGG 8 cut(s) 116, 413, 440, 569, 718, 773, 962, 1135
BmsI GCATC 2 cut(s) 643, 1093
BmtI GCTAGC 2 cut(s) 581, 794
BpmI CTGGAG 1 cut(s) 185
BpuMI CCSGG 4 cut(s) 413, 569, 718, 962
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 568, 996
BsaWI WCCGGW 1 cut(s) 507
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 535, 968
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 4 cut(s) 168, 607, 1058, 1076
Bse21I CCTNAGG 1 cut(s) 1113
Bse3DI GCAATG 2 cut(s) 470, 1006
BseBI CCWGG 4 cut(s) 116, 440, 773, 1135
BseDI CCNNGG 2 cut(s) 568, 996
BseGI GGATG 3 cut(s) 213, 409, 1108
BseLI CCNNNNNNNGG 3 cut(s) 115, 535, 968
BseMI GCAATG 2 cut(s) 470, 1006
BseMII CTCAG 3 cut(s) 311, 1026, 1104
BseNI ACTGG 4 cut(s) 168, 607, 1058, 1076
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 3 cut(s) 346, 492, 1045
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 1 cut(s) 119
BshNI GGYRCC 1 cut(s) 538
BsiEI CGRYCG 1 cut(s) 552
BsiHKAI GWGCWC 3 cut(s) 108, 505, 679
BsiSI CCGG 6 cut(s) 412, 508, 568, 718, 787, 962
BslFI GGGAC 3 cut(s) 585, 598, 776
BslI CCNNNNNNNGG 3 cut(s) 115, 535, 968
BsmFI GGGAC 3 cut(s) 585, 598, 776
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 4 cut(s) 108, 505, 679, 962
Bsp143I GATC 4 cut(s) 40, 444, 630, 1130
BspACI CCGC 2 cut(s) 760, 811
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 3 cut(s) 312, 1025, 1105
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 540, 1132
BspOI GCTAGC 2 cut(s) 581, 794
BspPI GGATC 4 cut(s) 35, 439, 1125, 1138
BspQI GCTCTTC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 538
BsrBI CCGCTC 1 cut(s) 811
BsrDI GCAATG 2 cut(s) 470, 1006
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 4 cut(s) 168, 607, 1058, 1076
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 2 cut(s) 568, 996
BssMI GATC 4 cut(s) 40, 444, 630, 1130
BssNI GRCGYC 1 cut(s) 539
Bst2UI CCWGG 4 cut(s) 116, 440, 773, 1135
Bst4CI ACNGT 7 cut(s) 151, 384, 589, 712, 746, 1123, 1147
Bst6I CTCTTC 1 cut(s) 822
BstACI GRCGYC 1 cut(s) 539
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 5 cut(s) 579, 679, 792, 908, 990
BstDEI CTNAG 3 cut(s) 320, 1012, 1113
BstF5I GGATG 3 cut(s) 213, 409, 1108
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 2 cut(s) 96, 541
BstKTI GATC 4 cut(s) 43, 447, 633, 1133
BstMBI GATC 4 cut(s) 40, 444, 630, 1130
BstMCI CGRYCG 1 cut(s) 552
BstMWI GCNNNNNNNGC 6 cut(s) 91, 232, 331, 653, 674, 683
BstNI CCWGG 4 cut(s) 116, 440, 773, 1135
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 8 cut(s) 114, 411, 438, 567, 716, 771, 960, 1133
BstSLI GKGCMC 1 cut(s) 962
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 346, 492, 1045
BstX2I RGATCY 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 1130
Bsu36I CCTNAGG 1 cut(s) 1113
BsuI GTATCC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 3 cut(s) 213, 409, 1108
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 4 cut(s) 147, 317, 498, 765
Cac8I GCNNGC 5 cut(s) 579, 679, 792, 908, 990
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 2 cut(s) 96, 541
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 1 cut(s) 510
CsiI ACCWGGT 2 cut(s) 438, 771
Csp6I GTAC 4 cut(s) 75, 161, 328, 1022
CviAII CATG 7 cut(s) 269, 295, 398, 514, 620, 682, 907
CviQI GTAC 4 cut(s) 75, 161, 328, 1022
DdeI CTNAG 3 cut(s) 320, 1012, 1113
DinI GGCGCC 1 cut(s) 540
DpnI GATC 4 cut(s) 42, 446, 632, 1132
DpnII GATC 4 cut(s) 40, 444, 630, 1130
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Ecl136II GAGCTC 2 cut(s) 106, 503
Eco24I GRGCYC 2 cut(s) 108, 505
Eco47I GGWCC 1 cut(s) 510
Eco53kI GAGCTC 2 cut(s) 106, 503
Eco81I CCTNAGG 1 cut(s) 1113
EcoICRI GAGCTC 2 cut(s) 106, 503
EcoRI GAATTC 1 cut(s) 555
EcoRII CCWGG 4 cut(s) 114, 438, 771, 1133
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 2 cut(s) 108, 505
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
FaeI CATG 7 cut(s) 272, 298, 401, 517, 623, 685, 910
FaqI GGGAC 3 cut(s) 585, 598, 776
FatI CATG 7 cut(s) 268, 294, 397, 513, 619, 681, 906
FauI CCCGC 1 cut(s) 753
Fnu4HI GCNGC 3 cut(s) 335, 481, 1034
FokI GGATG 3 cut(s) 200, 416, 1115
FriOI GRGCYC 2 cut(s) 108, 505
Fsp4HI GCNGC 3 cut(s) 335, 481, 1034
FspBI CTAG 4 cut(s) 578, 665, 791, 1025
GlaI GCGC 2 cut(s) 95, 540
GluI GCNGC 3 cut(s) 335, 481, 1034
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 1 cut(s) 119
HapII CCGG 6 cut(s) 412, 508, 568, 718, 787, 962
HhaI GCGC 2 cut(s) 96, 541
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 7 cut(s) 272, 298, 401, 517, 623, 685, 910
Hin6I GCGC 2 cut(s) 94, 539
HinP1I GCGC 2 cut(s) 94, 539
HincII GTYRAC 2 cut(s) 436, 1081
HindII GTYRAC 2 cut(s) 436, 1081
HinfI GANTC 5 cut(s) 98, 210, 920, 972, 1069
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 6 cut(s) 412, 508, 568, 718, 787, 962
HphI GGTGA 4 cut(s) 211, 454, 746, 956
Hpy166II GTNNAC 3 cut(s) 436, 496, 1081
Hpy188I TCNGA 5 cut(s) 103, 554, 696, 1000, 1068
Hpy188III TCNNGA 3 cut(s) 35, 917, 1100
Hpy8I GTNNAC 3 cut(s) 436, 496, 1081
HpyAV CCTTC 2 cut(s) 54, 338
HpyCH4III ACNGT 7 cut(s) 151, 384, 589, 712, 746, 1123, 1147
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 8 cut(s) 200, 397, 647, 656, 800, 910, 1033, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 232, 331, 653, 674, 683
HpyF3I CTNAG 3 cut(s) 320, 1012, 1113
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 7 cut(s) 272, 298, 401, 517, 623, 685, 910
HspAI GCGC 2 cut(s) 94, 539
KasI GGCGCC 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 436
Kzo9I GATC 4 cut(s) 40, 444, 630, 1130
LguI GCTCTTC 1 cut(s) 822
LmnI GCTCC 4 cut(s) 111, 696, 845, 964
Lsp1109I GCAGC 3 cut(s) 346, 492, 1045
LweI GCATC 2 cut(s) 643, 1093
MabI ACCWGGT 2 cut(s) 438, 771
MaeI CTAG 4 cut(s) 578, 665, 791, 1025
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 13
MalI GATC 4 cut(s) 42, 446, 632, 1132
MbiI CCGCTC 1 cut(s) 811
MboI GATC 4 cut(s) 40, 444, 630, 1130
MboII GAAGA 2 cut(s) 625, 839
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 1130
MhlI GDGCHC 4 cut(s) 108, 505, 679, 962
MluCI AATT 4 cut(s) 9, 555, 1095, 1140
Mly113I GGCGCC 1 cut(s) 539
MlyI GAGTC 3 cut(s) 107, 204, 914
MmeI TCCRAC 2 cut(s) 532, 1046
Mph1103I ATGCAT 1 cut(s) 399
MseI TTAA 4 cut(s) 66, 435, 707, 1149
MslI CAYNNNNRTG 2 cut(s) 138, 402
MspI CCGG 6 cut(s) 412, 508, 568, 718, 787, 962
MspR9I CCNGG 8 cut(s) 116, 413, 440, 569, 718, 773, 962, 1135
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 4 cut(s) 116, 440, 773, 1135
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 6 cut(s) 91, 232, 331, 653, 674, 683
NarI GGCGCC 1 cut(s) 539
NciI CCSGG 4 cut(s) 413, 569, 718, 962
NdeII GATC 4 cut(s) 40, 444, 630, 1130
NheI GCTAGC 2 cut(s) 577, 790
NlaIII CATG 7 cut(s) 272, 298, 401, 517, 623, 685, 910
NlaIV GGNNCC 2 cut(s) 540, 1132
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 1 cut(s) 910
PaeI GCATGC 1 cut(s) 910
PciSI GCTCTTC 1 cut(s) 822
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 348
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 972, 1069
PflFI GACNNNGTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 1133
PkrI GCNGC 3 cut(s) 336, 482, 1035
PleI GAGTC 3 cut(s) 106, 204, 914
PluTI GGCGCC 1 cut(s) 542
PpsI GAGTC 3 cut(s) 106, 204, 914
Psp124BI GAGCTC 2 cut(s) 108, 505
Psp6I CCWGG 4 cut(s) 114, 438, 771, 1133
PspGI CCWGG 4 cut(s) 114, 438, 771, 1133
PspN4I GGNNCC 2 cut(s) 540, 1132
PspPI GGNCC 1 cut(s) 510
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 1130
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 4 cut(s) 76, 162, 329, 1023
RsaNI GTAC 4 cut(s) 75, 161, 328, 1022
RseI CAYNNNNRTG 2 cut(s) 138, 402
SacI GAGCTC 2 cut(s) 108, 505
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 4 cut(s) 66, 435, 707, 1149
SatI GCNGC 3 cut(s) 335, 481, 1034
Sau3AI GATC 4 cut(s) 40, 444, 630, 1130
Sau96I GGNCC 1 cut(s) 510
ScaI AGTACT 1 cut(s) 76
SchI GAGTC 3 cut(s) 107, 204, 914
ScrFI CCNGG 8 cut(s) 116, 413, 440, 569, 718, 773, 962, 1135
SduI GDGCHC 4 cut(s) 108, 505, 679, 962
SexAI ACCWGGT 2 cut(s) 438, 771
SfaNI GCATC 2 cut(s) 643, 1093
SfoI GGCGCC 1 cut(s) 540
SinI GGWCC 1 cut(s) 510
SmiMI CAYNNNNRTG 2 cut(s) 138, 402
SpeI ACTAGT 1 cut(s) 1024
SphI GCATGC 1 cut(s) 910
Sse9I AATT 4 cut(s) 9, 555, 1095, 1140
SsiI CCGC 2 cut(s) 760, 811
SspDI GGCGCC 1 cut(s) 538
SspMI CTAG 4 cut(s) 578, 665, 791, 1025
SstI GAGCTC 2 cut(s) 108, 505
StyD4I CCNGG 8 cut(s) 114, 411, 438, 567, 716, 771, 960, 1133
TaaI ACNGT 7 cut(s) 151, 384, 589, 712, 746, 1123, 1147
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 126, 531
TaqII GACCGA 1 cut(s) 538
TasI AATT 4 cut(s) 9, 555, 1095, 1140
TatI WGTACW 2 cut(s) 74, 1021
TfiI GAWTC 2 cut(s) 972, 1069
Tru1I TTAA 4 cut(s) 66, 435, 707, 1149
Tru9I TTAA 4 cut(s) 66, 435, 707, 1149
TscAI CASTG 4 cut(s) 154, 324, 498, 772
TseI GCWGC 3 cut(s) 334, 480, 1033
TspDTI ATGAA 5 cut(s) 530, 625, 753, 795, 995
TspGWI ACGGA 1 cut(s) 769
TspRI CASTG 4 cut(s) 154, 324, 498, 772
Tth111I GACNNNGTC 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 1 cut(s) 555
XceI RCATGY 1 cut(s) 910
XspI CTAG 4 cut(s) 578, 665, 791, 1025
ZrmI AGTACT 1 cut(s) 76
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.