Prupe.3G003800_v2.0.a1

NYN domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0011904
Physical Location & Seq
Reverse (-)
252459 .. 256505
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G003800.1

Sequence Viewer

Length: 1962 bp
ATGCGTGGGGATGTGAGCGGAAGCAGCATGGCGGCGTCGACGTCGATGGGTAAGGCGGAGACGCAGTACGAGAAGGCGAAGACGTCAGTGTGGTGGGACATAGAGAACTGCCAGGTCCCCAAGGTCTGCGACACCCACGCCATCGCCCAGAACATTAGTTCTGCTCTCGTCAAGATGAATTACTGTGGGCCCGTCTCCATCTCCGCCTACGGCGACACCAATCGCATCCCTGCTTCTGTTCAGCACGCGCTCTCCAGCACCGGCATCGCCCTCAACCACGTCCCCGCTGGTGTGAAAGATGCAAGTGACAAGAAGATTCTAGTTGATATGCTGTTCTGGGCAGTAGATAACCCTGCTCCAGCCAACTACCTGCTTATTTCTGGAGACAGGGATTTCTCCAATGCTCTACACCAGTTGCGCATGAGAAGATATAATATCCTTCTGGCACAACCACAAAAAGCTTCCGCACCCCTCATAGCTGCAGCAAAGAGTGTATGGCTTTGGACGAGCCTCTCAGCTGGAGGGCCCCCACTTTCAAGTGGGGAGTCATCTCAGCTTGCGAATGGTAACCATTCTTATAACCCTGAAATGCCACAGTACCCTTTGCCTGAAGCGTTCCAGATAAACCCGCCGCCAGTTTATTTTGAACAGCCTTCTATGGGAAATCAAAAGCCTTCTACAAATGGAAGGGTTGGTGATAGTAAAAATAAAGGGAAATCAAATCGGAAAAACCCAAATCAACCCAACATATCAAGAGCCTCGAGTGTGCCAGTTGGGAACCAAGATGACAAGAATAATGATTACCCTTACCAATCAGAACATACACATGCAAAGCAGTTTAAGAAAGCTCCGCACGAATTCTTTGGTGTTAGTGAGAATCTAGTCTCCACTAATAGGTCTACTCCCAATTTCTTCCCTGGAAATTCTGATCCTTCAGGTGGTAATGGCAATAATTTTCTAGGTCAGCAAAACCAATATCCTCCTCCGAGGCTGAACAACTTCCACGTGCAACCTAATGTTGGACTGGATAATATGTTCCCTCCTAATTCTCAAAGTCATGGTTTTCGACCTATTCCTCCCAGACCTGATGGCCTTAGATTTTCTTCAGCCCCACCTACGAATATGCCCGATATGAGTAAGCTAATTGTCTCCGAGTACAGCAATTATGCTCAAAATTCGCAAAGGTTCCCTCATCGAAATGGAGACGAGTTTAGTAGACCCAGGTCTAGCGAGTCACTAAATTCAGCTAGCCCATATGCATCTCATAAGGGCCATAATTTGCAGAGTGGCCAGGGGTTTCATCATGATTCTATGAACAACAGGTATCCTCGGGGTTCTGATTATCTGTCCTCTCAATCCTCCCCTGCCGTTGCAAACAACATTCCTAGTAATGGCATTTGGGGAACTCAAGGCTGCACACCATCTGAATACGTTCAAGGCCTTATTGGCGTTATTTTACTAGCCCTAAACACCTTGAAAGTTGAAAAAATTATGCCTACTGAAGCAAACATTACCGATTGCATTCGTTATGGTGATCTGAAACACCGCAATACCGATGTAAGGAAGGCCTTGGATTACGCAATAGAGCAACATATGGTAGTGAAGCAGAGCCTTGGTGCACTTCAGTTGTATGTTGGTAAAAATGAGCAATTATGGAAGTGTGTGAACCCTATAGGTGGTAATCTTAATCATTACCCTAAAGCCACATGGGATCAGATACAGAACTTTCTGAGTTCCTCAGATGGGCGATCAGCAATAATGGCTTCTCAATGCAGATATGAAGCAGCTATGATTTTGAGAAAAGCGTGCTCAGAAGGGCTTGCTTTGGGCAATGTACTTCAGATCTTGAATATGATAGTCAGCATGAAGAAATGGATCACTCATCATCCGTCAGGATGGCAACCGATTTCTATTACCCTCGAAGAGACTAAAGCGGAAATGGGTGCTGAACCTAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NYN OHA

Protein Analysis

654

Amino Acids

71.63

Weight (kDa)

8.86

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011904)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62200
fragaria_vesca FvH4_1g26400 FvH4_1g26400 FvH4_1g26400
malus_domestica MD09G1292400.v1.1 MD17G1283700.v1.1
prunus_persica Prupe.3G003800_v2.0.a1
pyrus_communis pycom09g19710 pycom17g27960
rosa_chinensis RchiOBHm_Chr3g0486181
rosa_laevigata RLG00000023095
rosa_multiflora Rmu_sc0000372.1_g000001
rosa_roxburghii Rroxscaffold_6G00396010
rosa_rugosa Rorug03G0223900
rosa_samantha Rh3AG273800 Rh3BG309100 Rh3CG307300 Rh3DG303700
rosa_wichuraiana Rw3G024260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AatII GACGTC 2 cut(s) 44, 86
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 378
AccBSI CCGCTC 1 cut(s) 18
AccI GTMKAC 3 cut(s) 38, 899, 1216
AccII CGCG 1 cut(s) 248
AclWI GGATC 3 cut(s) 923, 1719, 1883
AcoI YGGCCR 1 cut(s) 1288
AcsI RAATTY 4 cut(s) 857, 922, 1174, 1240
AcuI CTGAAG 6 cut(s) 630, 918, 1089, 1521, 1607, 1823
AcvI CACGTG 1 cut(s) 1006
AcyI GRCGYC 3 cut(s) 35, 41, 83
AfaI GTAC 5 cut(s) 68, 599, 1157, 1836, 1957
AfiI CCNNNNNNNGG 8 cut(s) 659, 894, 938, 1019, 1391, 1560, 1676, 1743
AgsI TTSAA 6 cut(s) 537, 647, 1436, 1477, 1484, 1849
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 4 cut(s) 111, 916, 1220, 1290
AloI GAACNNNNNNTCC 2 cut(s) 1019, 1051
AluBI AGCT 8 cut(s) 461, 479, 518, 556, 848, 1141, 1247, 1787
AluI AGCT 8 cut(s) 461, 479, 518, 556, 848, 1141, 1247, 1787
Alw21I GWGCWC 2 cut(s) 1621, 1811
Alw26I GTCTC 7 cut(s) 53, 199, 378, 889, 1153, 1197, 1919
Alw44I GTGCAC 1 cut(s) 1617
AlwI GGATC 3 cut(s) 923, 1719, 1883
Ama87I CYCGRG 2 cut(s) 760, 1329
AoxI GGCC 7 cut(s) 188, 524, 1090, 1270, 1288, 1438, 1566
ApaI GGGCCC 2 cut(s) 192, 528
ApaLI GTGCAC 1 cut(s) 1617
ApeKI GCWGC 5 cut(s) 24, 479, 482, 1413, 1784
ApoI RAATTY 4 cut(s) 857, 922, 1174, 1240
Asp700I GAANNNNTTC 2 cut(s) 998, 1431
AspLEI GCGC 2 cut(s) 250, 420
AspS9I GGNCC 6 cut(s) 115, 188, 189, 524, 525, 1270
AsuHPI GGTGA 2 cut(s) 707, 1544
AsuNHI GCTAGC 1 cut(s) 1247
AvaI CYCGRG 2 cut(s) 760, 1329
AvaII GGWCC 1 cut(s) 115
BaeGI GKGCMC 3 cut(s) 192, 528, 1621
BalI TGGCCA 1 cut(s) 1290
BanII GRGCYC 2 cut(s) 192, 528
BbrPI CACGTG 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 2 cut(s) 1621, 1811
BbvI GCAGC 5 cut(s) 36, 466, 494, 1400, 1796
BccI CCATC 7 cut(s) 40, 149, 206, 1082, 1429, 1736, 1890
BceAI ACGGC 2 cut(s) 226, 1352
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 4 cut(s) 113, 918, 1222, 1292
BciVI GTATCC 1 cut(s) 1335
BcoDI GTCTC 7 cut(s) 53, 199, 378, 889, 1153, 1197, 1919
BfaI CTAG 8 cut(s) 320, 881, 959, 1227, 1248, 1386, 1460, 1953
BfmI CTRYAG 2 cut(s) 480, 1671
BfuAI ACCTGC 1 cut(s) 378
BfuI GTATCC 1 cut(s) 1335
BglI GCCNNNNNGGC 1 cut(s) 1446
BglII AGATCT 1 cut(s) 1842
BisI GCNGC 7 cut(s) 25, 33, 480, 483, 632, 1414, 1785
BlsI GCNGC 7 cut(s) 26, 34, 481, 484, 633, 1415, 1786
BmcAI AGTACT 1 cut(s) 1957
Bme1390I CCNGG 4 cut(s) 113, 918, 1222, 1292
Bme18I GGWCC 1 cut(s) 115
BmeT110I CYCGRG 2 cut(s) 760, 1329
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 6 cut(s) 115, 188, 189, 524, 525, 1270
BmiI GGNNCC 6 cut(s) 117, 190, 526, 527, 779, 1187
BmrFI CCNGG 4 cut(s) 113, 918, 1222, 1292
BmsI GCATC 4 cut(s) 234, 273, 289, 1268
BmtI GCTAGC 1 cut(s) 1251
BoxI GACNNNNGTC 1 cut(s) 1222
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 4 cut(s) 238, 342, 402, 540
BpuEI CTTGAG 1 cut(s) 1392
BsaAI YACGTR 1 cut(s) 1006
BsaHI GRCGYC 3 cut(s) 35, 41, 83
BsaJI CCNNGG 8 cut(s) 120, 916, 986, 1220, 1291, 1328, 1569, 1612
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 236, 266
Bsc4I CCNNNNNNNGG 8 cut(s) 659, 894, 938, 1019, 1391, 1560, 1676, 1743
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 4 cut(s) 412, 635, 770, 1029
Bse3DI GCAATG 1 cut(s) 1837
BseBI CCWGG 4 cut(s) 113, 918, 1222, 1292
BseDI CCNNGG 8 cut(s) 120, 916, 986, 1220, 1291, 1328, 1569, 1612
BseGI GGATG 4 cut(s) 16, 225, 1885, 1901
BseLI CCNNNNNNNGG 8 cut(s) 659, 894, 938, 1019, 1391, 1560, 1676, 1743
BseMI GCAATG 1 cut(s) 1837
BseMII CTCAG 5 cut(s) 528, 566, 1721, 1752, 1824
BseNI ACTGG 4 cut(s) 412, 635, 770, 1029
BseRI GAGGAG 1 cut(s) 972
BseSI GKGCMC 3 cut(s) 192, 528, 1621
BseXI GCAGC 5 cut(s) 36, 466, 494, 1400, 1796
BsgI GTGCAG 1 cut(s) 1399
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 7 cut(s) 190, 526, 1092, 1272, 1290, 1440, 1568
BsiHKAI GWGCWC 2 cut(s) 1621, 1811
BsiHKCI CYCGRG 2 cut(s) 760, 1329
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 3 cut(s) 101, 110, 266
BslI CCNNNNNNNGG 8 cut(s) 659, 894, 938, 1019, 1391, 1560, 1676, 1743
BsmAI GTCTC 7 cut(s) 53, 199, 378, 889, 1153, 1197, 1919
BsmBI CGTCTC 3 cut(s) 53, 199, 1197
BsmFI GGGAC 3 cut(s) 101, 110, 266
BsmI GAATGC 1 cut(s) 1521
BsnI GGCC 7 cut(s) 190, 526, 1092, 1272, 1290, 1440, 1568
BsoBI CYCGRG 2 cut(s) 760, 1329
Bsp120I GGGCCC 2 cut(s) 188, 524
Bsp1286I GDGCHC 4 cut(s) 192, 528, 1621, 1811
Bsp143I GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
BspANI GGCC 7 cut(s) 190, 526, 1092, 1272, 1290, 1440, 1568
BspCNI CTCAG 5 cut(s) 527, 565, 1722, 1751, 1823
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 6 cut(s) 117, 190, 526, 527, 779, 1187
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 378
BspOI GCTAGC 1 cut(s) 1251
BspPI GGATC 3 cut(s) 923, 1719, 1883
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 1 cut(s) 1837
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 4 cut(s) 412, 635, 770, 1029
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 8 cut(s) 120, 916, 986, 1220, 1291, 1328, 1569, 1612
BssMI GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
BssNI GRCGYC 3 cut(s) 35, 41, 83
BssT1I CCWWGG 3 cut(s) 120, 1569, 1612
Bst2UI CCWGG 4 cut(s) 113, 918, 1222, 1292
Bst4CI ACNGT 2 cut(s) 185, 597
Bst6I CTCTTC 1 cut(s) 1917
BstACI GRCGYC 3 cut(s) 35, 41, 83
BstBAI YACGTR 1 cut(s) 1006
BstC8I GCNNGC 5 cut(s) 246, 558, 1249, 1807, 1821
BstDEI CTNAG 6 cut(s) 514, 552, 1094, 1730, 1738, 1810
BstEII GGTNACC 1 cut(s) 566
BstF5I GGATG 4 cut(s) 16, 225, 1885, 1901
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 2 cut(s) 250, 420
BstKTI GATC 6 cut(s) 931, 1537, 1714, 1751, 1845, 1878
BstMAI GTCTC 7 cut(s) 53, 199, 378, 889, 1153, 1197, 1919
BstMBI GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
BstMWI GCNNNNNNNGC 3 cut(s) 24, 1446, 1760
BstNI CCWGG 4 cut(s) 113, 918, 1222, 1292
BstNSI RCATGY 1 cut(s) 830
BstPAI GACNNNNGTC 1 cut(s) 1222
BstPI GGTNACC 1 cut(s) 566
BstSCI CCNGG 4 cut(s) 111, 916, 1220, 1290
BstSFI CTRYAG 2 cut(s) 480, 1671
BstSLI GKGCMC 3 cut(s) 192, 528, 1621
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 5 cut(s) 36, 466, 494, 1400, 1796
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 1842
BstYI RGATCY 1 cut(s) 1842
BsuI GTATCC 1 cut(s) 1335
BsuRI GGCC 7 cut(s) 190, 526, 1092, 1272, 1290, 1440, 1568
BtgZI GCGATG 2 cut(s) 127, 250
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 4 cut(s) 16, 225, 1885, 1901
BtsIMutI CAGTG 1 cut(s) 93
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 5 cut(s) 246, 558, 1249, 1807, 1821
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 2 cut(s) 250, 420
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 115, 188, 189, 524, 525, 1270
CseI GACGC 2 cut(s) 24, 70
Csp6I GTAC 5 cut(s) 67, 598, 1156, 1835, 1956
CviAII CATG 7 cut(s) 28, 421, 827, 1058, 1304, 1707, 1864
CviQI GTAC 5 cut(s) 67, 598, 1156, 1835, 1956
DdeI CTNAG 6 cut(s) 514, 552, 1094, 1730, 1738, 1810
DpnI GATC 6 cut(s) 930, 1536, 1713, 1750, 1844, 1877
DpnII GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
EaeI YGGCCR 1 cut(s) 1288
Eam1104I CTCTTC 1 cut(s) 1917
EarI CTCTTC 1 cut(s) 1917
EciI GGCGGA 2 cut(s) 71, 193
Eco130I CCWWGG 3 cut(s) 120, 1569, 1612
Eco147I AGGCCT 2 cut(s) 1440, 1568
Eco24I GRGCYC 2 cut(s) 192, 528
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 6 cut(s) 630, 918, 1089, 1521, 1607, 1823
Eco72I CACGTG 1 cut(s) 1006
Eco88I CYCGRG 2 cut(s) 760, 1329
Eco91I GGTNACC 1 cut(s) 566
EcoO109I RGGNCCY 3 cut(s) 115, 524, 525
EcoO65I GGTNACC 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 4 cut(s) 111, 916, 1220, 1290
EcoT14I CCWWGG 3 cut(s) 120, 1569, 1612
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 2 cut(s) 192, 528
ErhI CCWWGG 3 cut(s) 120, 1569, 1612
Esp3I CGTCTC 3 cut(s) 53, 199, 1197
FaeI CATG 7 cut(s) 31, 424, 830, 1061, 1307, 1710, 1867
FaqI GGGAC 3 cut(s) 101, 110, 266
FatI CATG 7 cut(s) 27, 420, 826, 1057, 1303, 1706, 1863
FauI CCCGC 2 cut(s) 292, 636
FauNDI CATATG 2 cut(s) 1255, 1593
FblI GTMKAC 3 cut(s) 38, 899, 1216
Fnu4HI GCNGC 7 cut(s) 25, 33, 480, 483, 632, 1414, 1785
FokI GGATG 4 cut(s) 23, 212, 1872, 1908
FriOI GRGCYC 2 cut(s) 192, 528
Fsp4HI GCNGC 7 cut(s) 25, 33, 480, 483, 632, 1414, 1785
FspBI CTAG 8 cut(s) 320, 881, 959, 1227, 1248, 1386, 1460, 1953
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 2 cut(s) 249, 419
GluI GCNGC 7 cut(s) 25, 33, 480, 483, 632, 1414, 1785
GsuI CTGGAG 4 cut(s) 238, 342, 402, 540
HaeIII GGCC 7 cut(s) 190, 526, 1092, 1272, 1290, 1440, 1568
HapII CCGG 1 cut(s) 261
HgaI GACGC 2 cut(s) 24, 70
HhaI GCGC 2 cut(s) 250, 420
Hin1I GRCGYC 3 cut(s) 35, 41, 83
Hin1II CATG 7 cut(s) 31, 424, 830, 1061, 1307, 1710, 1867
Hin6I GCGC 2 cut(s) 248, 418
HinP1I GCGC 2 cut(s) 248, 418
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 5 cut(s) 316, 545, 877, 1232, 1307
HpaII CCGG 1 cut(s) 261
HphI GGTGA 2 cut(s) 707, 1544
Hpy166II GTNNAC 5 cut(s) 39, 900, 1217, 1619, 1666
Hpy188III TCNNGA 7 cut(s) 172, 381, 619, 753, 1304, 1846, 1893
Hpy8I GTNNAC 5 cut(s) 39, 900, 1217, 1619, 1666
Hpy99I CGWCG 3 cut(s) 40, 43, 46
HpyAV CCTTC 8 cut(s) 67, 449, 663, 681, 684, 942, 1558, 1808
HpyCH4III ACNGT 2 cut(s) 185, 597
HpyCH4IV ACGT 5 cut(s) 41, 83, 279, 1005, 1431
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 1446, 1760
HpyF3I CTNAG 6 cut(s) 514, 552, 1094, 1730, 1738, 1810
HpySE526I ACGT 5 cut(s) 41, 83, 279, 1005, 1431
Hsp92I GRCGYC 3 cut(s) 35, 41, 83
Hsp92II CATG 7 cut(s) 31, 424, 830, 1061, 1307, 1710, 1867
HspAI GCGC 2 cut(s) 248, 418
Kzo9I GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
LmnI GCTCC 2 cut(s) 361, 853
Lsp1109I GCAGC 5 cut(s) 36, 466, 494, 1400, 1796
LweI GCATC 4 cut(s) 234, 273, 289, 1268
MaeI CTAG 8 cut(s) 320, 881, 959, 1227, 1248, 1386, 1460, 1953
MaeII ACGT 5 cut(s) 41, 83, 279, 1005, 1431
MaeIII GTNAC 3 cut(s) 305, 566, 1233
MalI GATC 6 cut(s) 930, 1536, 1713, 1750, 1844, 1877
MbiI CCGCTC 1 cut(s) 18
MboI GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
MboII GAAGA 7 cut(s) 91, 325, 438, 904, 1095, 1879, 1934
MflI RGATCY 1 cut(s) 1842
MhlI GDGCHC 4 cut(s) 192, 528, 1621, 1811
MlsI TGGCCA 1 cut(s) 1290
MluNI TGGCCA 1 cut(s) 1290
MlyI GAGTC 2 cut(s) 554, 1241
MmeI TCCRAC 1 cut(s) 1000
Mox20I TGGCCA 1 cut(s) 1290
Mph1103I ATGCAT 1 cut(s) 1261
MroXI GAANNNNTTC 2 cut(s) 998, 1431
MscI TGGCCA 1 cut(s) 1290
MseI TTAA 2 cut(s) 840, 1686
MslI CAYNNNNRTG 2 cut(s) 825, 1197
Msp20I TGGCCA 1 cut(s) 1290
MspA1I CMGCKG 2 cut(s) 287, 518
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 4 cut(s) 113, 918, 1222, 1292
Mva1269I GAATGC 1 cut(s) 1521
MvaI CCWGG 4 cut(s) 113, 918, 1222, 1292
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 24, 1446, 1760
NdeI CATATG 2 cut(s) 1255, 1593
NdeII GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
NheI GCTAGC 1 cut(s) 1247
NlaIII CATG 7 cut(s) 31, 424, 830, 1061, 1307, 1710, 1867
NlaIV GGNNCC 6 cut(s) 117, 190, 526, 527, 779, 1187
NmuCI GTSAC 2 cut(s) 305, 1233
NsbI TGCGCA 1 cut(s) 419
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 1 cut(s) 830
PaeR7I CTCGAG 1 cut(s) 760
PagI TCATGA 1 cut(s) 1303
PceI AGGCCT 2 cut(s) 1440, 1568
PctI GAATGC 1 cut(s) 1521
PdmI GAANNNNTTC 2 cut(s) 998, 1431
PfeI GAWTC 3 cut(s) 316, 877, 1307
PkrI GCNGC 7 cut(s) 26, 34, 481, 484, 633, 1415, 1786
PleI GAGTC 2 cut(s) 553, 1240
PmaCI CACGTG 1 cut(s) 1006
PmlI CACGTG 1 cut(s) 1006
PpsI GAGTC 2 cut(s) 553, 1240
Ppu21I YACGTR 1 cut(s) 1006
PpuMI RGGWCCY 1 cut(s) 115
PshAI GACNNNNGTC 1 cut(s) 1222
PsiI TTATAA 1 cut(s) 579
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 111, 916, 1220, 1290
PspCI CACGTG 1 cut(s) 1006
PspEI GGTNACC 1 cut(s) 566
PspGI CCWGG 4 cut(s) 111, 916, 1220, 1290
PspN4I GGNNCC 6 cut(s) 117, 190, 526, 527, 779, 1187
PspOMI GGGCCC 2 cut(s) 188, 524
PspPI GGNCC 6 cut(s) 115, 188, 189, 524, 525, 1270
PspPPI RGGWCCY 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 760
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 1842
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 5 cut(s) 68, 599, 1157, 1836, 1957
RsaNI GTAC 5 cut(s) 67, 598, 1156, 1835, 1956
RseI CAYNNNNRTG 2 cut(s) 825, 1197
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 2 cut(s) 840, 1686
SatI GCNGC 7 cut(s) 25, 33, 480, 483, 632, 1414, 1785
Sau3AI GATC 6 cut(s) 928, 1534, 1711, 1748, 1842, 1875
Sau96I GGNCC 6 cut(s) 115, 188, 189, 524, 525, 1270
ScaI AGTACT 1 cut(s) 1957
SchI GAGTC 2 cut(s) 554, 1241
ScrFI CCNGG 4 cut(s) 113, 918, 1222, 1292
SduI GDGCHC 4 cut(s) 192, 528, 1621, 1811
SfaNI GCATC 4 cut(s) 234, 273, 289, 1268
SfcI CTRYAG 2 cut(s) 480, 1671
Sfr274I CTCGAG 1 cut(s) 760
SgrDI CGTCGACG 1 cut(s) 37
SinI GGWCC 1 cut(s) 115
SlaI CTCGAG 1 cut(s) 760
SmiMI CAYNNNNRTG 2 cut(s) 825, 1197
SmlI CTYRAG 2 cut(s) 760, 1407
SmoI CTYRAG 2 cut(s) 760, 1407
SseBI AGGCCT 2 cut(s) 1440, 1568
SspMI CTAG 8 cut(s) 320, 881, 959, 1227, 1248, 1386, 1460, 1953
StuI AGGCCT 2 cut(s) 1440, 1568
StyD4I CCNGG 4 cut(s) 111, 916, 1220, 1290
StyI CCWWGG 3 cut(s) 120, 1569, 1612
TaaI ACNGT 2 cut(s) 185, 597
TaiI ACGT 5 cut(s) 44, 86, 282, 1008, 1434
TaqI TCGA 6 cut(s) 38, 44, 761, 1066, 1195, 1920
TatI WGTACW 3 cut(s) 1155, 1834, 1955
TauI GCSGC 2 cut(s) 35, 634
TfiI GAWTC 3 cut(s) 316, 877, 1307
Tru1I TTAA 2 cut(s) 840, 1686
Tru9I TTAA 2 cut(s) 840, 1686
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 2 cut(s) 305, 1233
TseI GCWGC 5 cut(s) 24, 479, 482, 1413, 1784
Tsp45I GTSAC 2 cut(s) 305, 1233
TspDTI ATGAA 5 cut(s) 191, 1289, 1328, 1794, 1880
TspGWI ACGGA 1 cut(s) 1878
TspRI CASTG 1 cut(s) 93
VneI GTGCAC 1 cut(s) 1617
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 4 cut(s) 857, 922, 1174, 1240
XceI RCATGY 1 cut(s) 830
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XhoI CTCGAG 1 cut(s) 760
XmiI GTMKAC 3 cut(s) 38, 899, 1216
XmnI GAANNNNTTC 2 cut(s) 998, 1431
XspI CTAG 8 cut(s) 320, 881, 959, 1227, 1248, 1386, 1460, 1953
ZraI GACGTC 2 cut(s) 42, 84
ZrmI AGTACT 1 cut(s) 1957
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.