protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 272, 387 |
| AciI | CCGC | 3 cut(s) | 111, 129, 385 |
| AclI | AACGTT | 1 cut(s) | 66 |
| AclWI | GGATC | 1 cut(s) | 365 |
| AcuI | CTGAAG | 2 cut(s) | 121, 461 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 160, 161 |
| AflIII | ACRYGT | 2 cut(s) | 270, 608 |
| AgsI | TTSAA | 3 cut(s) | 20, 488, 515 |
| AjnI | CCWGG | 2 cut(s) | 84, 585 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 203, 235 |
| AluBI | AGCT | 2 cut(s) | 317, 428 |
| AluI | AGCT | 2 cut(s) | 317, 428 |
| AlwI | GGATC | 1 cut(s) | 365 |
| Ama87I | CYCGRG | 1 cut(s) | 292 |
| AoxI | GGCC | 1 cut(s) | 444 |
| ApeKI | GCWGC | 1 cut(s) | 428 |
| AspLEI | GCGC | 1 cut(s) | 389 |
| AspS9I | GGNCC | 1 cut(s) | 188 |
| AsuC2I | CCSGG | 3 cut(s) | 173, 293, 294 |
| AsuHPI | GGTGA | 1 cut(s) | 196 |
| AvaI | CYCGRG | 1 cut(s) | 292 |
| AvaII | GGWCC | 1 cut(s) | 188 |
| BanII | GRGCYC | 1 cut(s) | 597 |
| BbvI | GCAGC | 1 cut(s) | 415 |
| BciT130I | CCWGG | 2 cut(s) | 86, 587 |
| BclI | TGATCA | 1 cut(s) | 42 |
| BcnI | CCSGG | 3 cut(s) | 173, 293, 294 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BisI | GCNGC | 2 cut(s) | 112, 429 |
| BlsI | GCNGC | 2 cut(s) | 113, 430 |
| Bme1390I | CCNGG | 5 cut(s) | 86, 173, 293, 294, 587 |
| Bme18I | GGWCC | 1 cut(s) | 188 |
| BmeT110I | CYCGRG | 1 cut(s) | 292 |
| BmgT120I | GGNCC | 1 cut(s) | 188 |
| BmrFI | CCNGG | 5 cut(s) | 86, 173, 293, 294, 587 |
| BmrI | ACTGGG | 2 cut(s) | 359, 408 |
| BmuI | ACTGGG | 2 cut(s) | 359, 408 |
| BoxI | GACNNNNGTC | 1 cut(s) | 200 |
| BpuMI | CCSGG | 3 cut(s) | 173, 293, 294 |
| BsaJI | CCNNGG | 2 cut(s) | 292, 293 |
| BsaWI | WCCGGW | 1 cut(s) | 412 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 160, 161 |
| Bse1I | ACTGG | 3 cut(s) | 365, 374, 403 |
| BseBI | CCWGG | 2 cut(s) | 86, 587 |
| BseDI | CCNNGG | 2 cut(s) | 292, 293 |
| BseGI | GGATG | 1 cut(s) | 595 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 160, 161 |
| BseNI | ACTGG | 3 cut(s) | 365, 374, 403 |
| BseXI | GCAGC | 1 cut(s) | 415 |
| Bsh1236I | CGCG | 2 cut(s) | 272, 387 |
| Bsh1285I | CGRYCG | 1 cut(s) | 451 |
| BshFI | GGCC | 1 cut(s) | 446 |
| BsiEI | CGRYCG | 1 cut(s) | 451 |
| BsiHKCI | CYCGRG | 1 cut(s) | 292 |
| BsiSI | CCGG | 3 cut(s) | 172, 293, 413 |
| BslFI | GGGAC | 1 cut(s) | 345 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 160, 161 |
| BsmFI | GGGAC | 1 cut(s) | 345 |
| BsnI | GGCC | 1 cut(s) | 446 |
| BsoBI | CYCGRG | 1 cut(s) | 292 |
| Bsp1286I | GDGCHC | 1 cut(s) | 597 |
| Bsp143I | GATC | 4 cut(s) | 42, 60, 370, 448 |
| BspACI | CCGC | 3 cut(s) | 111, 129, 385 |
| BspANI | GGCC | 1 cut(s) | 446 |
| BspFNI | CGCG | 2 cut(s) | 272, 387 |
| BspPI | GGATC | 1 cut(s) | 365 |
| BsrI | ACTGG | 3 cut(s) | 365, 374, 403 |
| BssECI | CCNNGG | 2 cut(s) | 292, 293 |
| BssMI | GATC | 4 cut(s) | 42, 60, 370, 448 |
| Bst2UI | CCWGG | 2 cut(s) | 86, 587 |
| Bst4CI | ACNGT | 2 cut(s) | 185, 340 |
| BstC8I | GCNNGC | 2 cut(s) | 274, 319 |
| BstDEI | CTNAG | 2 cut(s) | 80, 192 |
| BstF5I | GGATG | 1 cut(s) | 595 |
| BstFNI | CGCG | 2 cut(s) | 272, 387 |
| BstHHI | GCGC | 1 cut(s) | 389 |
| BstKTI | GATC | 4 cut(s) | 45, 63, 373, 451 |
| BstMBI | GATC | 4 cut(s) | 42, 60, 370, 448 |
| BstMCI | CGRYCG | 1 cut(s) | 451 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 386, 434, 443 |
| BstNI | CCWGG | 2 cut(s) | 86, 587 |
| BstNSI | RCATGY | 1 cut(s) | 612 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 200 |
| BstSCI | CCNGG | 5 cut(s) | 84, 171, 291, 292, 585 |
| BstUI | CGCG | 2 cut(s) | 272, 387 |
| BstV1I | GCAGC | 1 cut(s) | 415 |
| BsuRI | GGCC | 1 cut(s) | 446 |
| BtsCI | GGATG | 1 cut(s) | 595 |
| BtsIMutI | CAGTG | 1 cut(s) | 412 |
| Cac8I | GCNNGC | 2 cut(s) | 274, 319 |
| CfoI | GCGC | 1 cut(s) | 389 |
| Cfr13I | GGNCC | 1 cut(s) | 188 |
| Cfr9I | CCCGGG | 1 cut(s) | 292 |
| CviAII | CATG | 2 cut(s) | 352, 609 |
| DdeI | CTNAG | 2 cut(s) | 80, 192 |
| DpnI | GATC | 4 cut(s) | 44, 62, 372, 450 |
| DpnII | GATC | 4 cut(s) | 42, 60, 370, 448 |
| Eco24I | GRGCYC | 1 cut(s) | 597 |
| Eco47I | GGWCC | 1 cut(s) | 188 |
| Eco57I | CTGAAG | 2 cut(s) | 121, 461 |
| Eco88I | CYCGRG | 1 cut(s) | 292 |
| EcoRII | CCWGG | 2 cut(s) | 84, 585 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| EcoT38I | GRGCYC | 1 cut(s) | 597 |
| FaeI | CATG | 2 cut(s) | 355, 612 |
| FaiI | YATR | 9 cut(s) | 6, 102, 151, 201, 237, 348, 353, 600, 610 |
| FaqI | GGGAC | 1 cut(s) | 345 |
| FatI | CATG | 2 cut(s) | 351, 608 |
| FauI | CCCGC | 1 cut(s) | 392 |
| FbaI | TGATCA | 1 cut(s) | 42 |
| Fnu4HI | GCNGC | 2 cut(s) | 112, 429 |
| FokI | GGATG | 1 cut(s) | 602 |
| FriOI | GRGCYC | 1 cut(s) | 597 |
| Fsp4HI | GCNGC | 2 cut(s) | 112, 429 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GlaI | GCGC | 1 cut(s) | 388 |
| GluI | GCNGC | 2 cut(s) | 112, 429 |
| HaeIII | GGCC | 1 cut(s) | 446 |
| HapII | CCGG | 3 cut(s) | 172, 293, 413 |
| HhaI | GCGC | 1 cut(s) | 389 |
| Hin1II | CATG | 2 cut(s) | 355, 612 |
| Hin6I | GCGC | 1 cut(s) | 387 |
| HinP1I | GCGC | 1 cut(s) | 387 |
| HindIII | AAGCTT | 1 cut(s) | 315 |
| HinfI | GANTC | 1 cut(s) | 533 |
| HpaII | CCGG | 3 cut(s) | 172, 293, 413 |
| HphI | GGTGA | 1 cut(s) | 196 |
| Hpy166II | GTNNAC | 1 cut(s) | 169 |
| Hpy188I | TCNGA | 2 cut(s) | 140, 441 |
| Hpy188III | TCNNGA | 1 cut(s) | 537 |
| Hpy8I | GTNNAC | 1 cut(s) | 169 |
| HpyAV | CCTTC | 2 cut(s) | 20, 436 |
| HpyCH4III | ACNGT | 2 cut(s) | 185, 340 |
| HpyCH4IV | ACGT | 1 cut(s) | 66 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 276, 321, 499, 519, 602 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 386, 434, 443 |
| HpyF3I | CTNAG | 2 cut(s) | 80, 192 |
| HpySE526I | ACGT | 1 cut(s) | 66 |
| Hsp92II | CATG | 2 cut(s) | 355, 612 |
| HspAI | GCGC | 1 cut(s) | 387 |
| Ksp22I | TGATCA | 1 cut(s) | 42 |
| Kzo9I | GATC | 4 cut(s) | 42, 60, 370, 448 |
| Lsp1109I | GCAGC | 1 cut(s) | 415 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MaeII | ACGT | 1 cut(s) | 66 |
| MaeIII | GTNAC | 1 cut(s) | 202 |
| MalI | GATC | 4 cut(s) | 44, 62, 372, 450 |
| MboI | GATC | 4 cut(s) | 42, 60, 370, 448 |
| MboII | GAAGA | 1 cut(s) | 128 |
| MhlI | GDGCHC | 1 cut(s) | 597 |
| MluCI | AATT | 2 cut(s) | 178, 488 |
| MluI | ACGCGT | 1 cut(s) | 270 |
| MnlI | CCTC | 1 cut(s) | 533 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 4 cut(s) | 74, 123, 300, 555 |
| MspA1I | CMGCKG | 1 cut(s) | 428 |
| MspI | CCGG | 3 cut(s) | 172, 293, 413 |
| MspR9I | CCNGG | 5 cut(s) | 86, 173, 293, 294, 587 |
| MvaI | CCWGG | 2 cut(s) | 86, 587 |
| MvnI | CGCG | 2 cut(s) | 272, 387 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 386, 434, 443 |
| NciI | CCSGG | 3 cut(s) | 173, 293, 294 |
| NdeII | GATC | 4 cut(s) | 42, 60, 370, 448 |
| NlaIII | CATG | 2 cut(s) | 355, 612 |
| NmuCI | GTSAC | 1 cut(s) | 202 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 612 |
| PciI | ACATGT | 1 cut(s) | 608 |
| PfeI | GAWTC | 1 cut(s) | 533 |
| PkrI | GCNGC | 2 cut(s) | 113, 430 |
| Ple19I | CGATCG | 1 cut(s) | 451 |
| PscI | ACATGT | 1 cut(s) | 608 |
| PshAI | GACNNNNGTC | 1 cut(s) | 200 |
| Psp1406I | AACGTT | 1 cut(s) | 66 |
| Psp6I | CCWGG | 2 cut(s) | 84, 585 |
| PspGI | CCWGG | 2 cut(s) | 84, 585 |
| PspPI | GGNCC | 1 cut(s) | 188 |
| PvuI | CGATCG | 1 cut(s) | 451 |
| PvuII | CAGCTG | 1 cut(s) | 428 |
| SaqAI | TTAA | 4 cut(s) | 74, 123, 300, 555 |
| SatI | GCNGC | 2 cut(s) | 112, 429 |
| Sau3AI | GATC | 4 cut(s) | 42, 60, 370, 448 |
| Sau96I | GGNCC | 1 cut(s) | 188 |
| ScrFI | CCNGG | 5 cut(s) | 86, 173, 293, 294, 587 |
| SduI | GDGCHC | 1 cut(s) | 597 |
| SetI | ASST | 7 cut(s) | 69, 258, 263, 319, 347, 430, 478 |
| SinI | GGWCC | 1 cut(s) | 188 |
| SmaI | CCCGGG | 1 cut(s) | 294 |
| Sse9I | AATT | 2 cut(s) | 178, 488 |
| SsiI | CCGC | 3 cut(s) | 111, 129, 385 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyD4I | CCNGG | 5 cut(s) | 84, 171, 291, 292, 585 |
| TaaI | ACNGT | 2 cut(s) | 185, 340 |
| TaiI | ACGT | 1 cut(s) | 69 |
| TaqI | TCGA | 2 cut(s) | 59, 451 |
| TasI | AATT | 2 cut(s) | 178, 488 |
| TauI | GCSGC | 1 cut(s) | 114 |
| TfiI | GAWTC | 1 cut(s) | 533 |
| Tru1I | TTAA | 4 cut(s) | 74, 123, 300, 555 |
| Tru9I | TTAA | 4 cut(s) | 74, 123, 300, 555 |
| TscAI | CASTG | 1 cut(s) | 412 |
| TseFI | GTSAC | 1 cut(s) | 202 |
| TseI | GCWGC | 1 cut(s) | 428 |
| Tsp45I | GTSAC | 1 cut(s) | 202 |
| TspDTI | ATGAA | 1 cut(s) | 108 |
| TspMI | CCCGGG | 1 cut(s) | 292 |
| TspRI | CASTG | 1 cut(s) | 412 |
| VpaK11BI | GGWCC | 1 cut(s) | 188 |
| XceI | RCATGY | 1 cut(s) | 612 |
| XmaI | CCCGGG | 1 cut(s) | 292 |
| XspI | CTAG | 1 cut(s) | 306 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |