protein serine/threonine kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 225 |
| Acc16I | TGCGCA | 1 cut(s) | 724 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 512 |
| AccI | GTMKAC | 1 cut(s) | 606 |
| AciI | CCGC | 2 cut(s) | 602, 777 |
| AclI | AACGTT | 1 cut(s) | 434 |
| AcsI | RAATTY | 3 cut(s) | 258, 283, 700 |
| AcyI | GRCGYC | 2 cut(s) | 135, 222 |
| AfaI | GTAC | 2 cut(s) | 523, 763 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 161, 162, 512 |
| AflIII | ACRYGT | 2 cut(s) | 482, 746 |
| AgsI | TTSAA | 2 cut(s) | 235, 768 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 498 |
| AhlI | ACTAGT | 1 cut(s) | 758 |
| AjiI | CACGTC | 1 cut(s) | 485 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 12, 44 |
| AluBI | AGCT | 4 cut(s) | 176, 252, 465, 560 |
| AluI | AGCT | 4 cut(s) | 176, 252, 465, 560 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 330 |
| ApeKI | GCWGC | 3 cut(s) | 111, 114, 557 |
| ApoI | RAATTY | 3 cut(s) | 258, 283, 700 |
| AseI | ATTAAT | 1 cut(s) | 810 |
| AspLEI | GCGC | 2 cut(s) | 248, 725 |
| AspS9I | GGNCC | 3 cut(s) | 205, 291, 516 |
| AsuC2I | CCSGG | 1 cut(s) | 576 |
| AsuHPI | GGTGA | 2 cut(s) | 502, 586 |
| AvaII | GGWCC | 3 cut(s) | 205, 291, 516 |
| BanII | GRGCYC | 1 cut(s) | 576 |
| BbvI | GCAGC | 3 cut(s) | 123, 126, 569 |
| BccI | CCATC | 1 cut(s) | 514 |
| BclI | TGATCA | 1 cut(s) | 91 |
| BcnI | CCSGG | 1 cut(s) | 576 |
| BcuI | ACTAGT | 1 cut(s) | 758 |
| BfaI | CTAG | 4 cut(s) | 177, 215, 306, 759 |
| BfmI | CTRYAG | 3 cut(s) | 109, 253, 567 |
| BisI | GCNGC | 3 cut(s) | 112, 115, 558 |
| BlsI | GCNGC | 3 cut(s) | 113, 116, 559 |
| BmcAI | AGTACT | 1 cut(s) | 763 |
| Bme1390I | CCNGG | 1 cut(s) | 576 |
| Bme18I | GGWCC | 3 cut(s) | 205, 291, 516 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 498 |
| BmgBI | CACGTC | 1 cut(s) | 485 |
| BmgT120I | GGNCC | 3 cut(s) | 205, 291, 516 |
| BmiI | GGNNCC | 1 cut(s) | 517 |
| BmrFI | CCNGG | 1 cut(s) | 576 |
| BmrI | ACTGGG | 1 cut(s) | 328 |
| BmsI | GCATC | 2 cut(s) | 589, 749 |
| BmuI | ACTGGG | 1 cut(s) | 328 |
| BoxI | GACNNNNGTC | 1 cut(s) | 344 |
| BpuMI | CCSGG | 1 cut(s) | 576 |
| BsaBI | GATNNNNATC | 1 cut(s) | 65 |
| BsaHI | GRCGYC | 2 cut(s) | 135, 222 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 161, 162, 512 |
| Bse1I | ACTGG | 2 cut(s) | 323, 803 |
| Bse8I | GATNNNNATC | 1 cut(s) | 65 |
| BseGI | GGATG | 1 cut(s) | 740 |
| BseJI | GATNNNNATC | 1 cut(s) | 65 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 161, 162, 512 |
| BseNI | ACTGG | 2 cut(s) | 323, 803 |
| BseXI | GCAGC | 3 cut(s) | 123, 126, 569 |
| BsiSI | CCGG | 2 cut(s) | 195, 576 |
| BslFI | GGGAC | 2 cut(s) | 209, 529 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 161, 162, 512 |
| BsmFI | GGGAC | 2 cut(s) | 209, 529 |
| Bsp1286I | GDGCHC | 1 cut(s) | 576 |
| Bsp1407I | TGTACA | 1 cut(s) | 521 |
| Bsp143I | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| BspACI | CCGC | 2 cut(s) | 602, 777 |
| BspLI | GGNNCC | 1 cut(s) | 517 |
| BspMAI | CTGCAG | 1 cut(s) | 113 |
| BsrGI | TGTACA | 1 cut(s) | 521 |
| BsrI | ACTGG | 2 cut(s) | 323, 803 |
| BssMI | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| BssNI | GRCGYC | 2 cut(s) | 135, 222 |
| Bst4CI | ACNGT | 4 cut(s) | 185, 295, 496, 678 |
| BstACI | GRCGYC | 2 cut(s) | 135, 222 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 563 |
| BstAUI | TGTACA | 1 cut(s) | 521 |
| BstC8I | GCNNGC | 2 cut(s) | 250, 804 |
| BstF5I | GGATG | 1 cut(s) | 740 |
| BstHHI | GCGC | 2 cut(s) | 248, 725 |
| BstKTI | GATC | 6 cut(s) | 45, 63, 94, 594, 646, 730 |
| BstMBI | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 141, 462, 563, 731 |
| BstNSI | RCATGY | 2 cut(s) | 750, 806 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 344 |
| BstSCI | CCNGG | 1 cut(s) | 574 |
| BstSFI | CTRYAG | 3 cut(s) | 109, 253, 567 |
| BstV1I | GCAGC | 3 cut(s) | 123, 126, 569 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 798 |
| BtrI | CACGTC | 1 cut(s) | 485 |
| BtsCI | GGATG | 1 cut(s) | 740 |
| BtsI | GCAGTG | 2 cut(s) | 331, 620 |
| BtsIMutI | CAGTG | 3 cut(s) | 331, 620, 683 |
| Cac8I | GCNNGC | 2 cut(s) | 250, 804 |
| CaiI | CAGNNNCTG | 1 cut(s) | 330 |
| CfoI | GCGC | 2 cut(s) | 248, 725 |
| Cfr13I | GGNCC | 3 cut(s) | 205, 291, 516 |
| CseI | GACGC | 1 cut(s) | 124 |
| Csp6I | GTAC | 2 cut(s) | 522, 762 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 112, 147 |
| CviAII | CATG | 4 cut(s) | 238, 400, 747, 803 |
| CviJI | RGCY | 9 cut(s) | 176, 194, 252, 465, 505, 560, 574, 685, 734 |
| CviKI_1 | RGCY | 9 cut(s) | 176, 194, 252, 465, 505, 560, 574, 685, 734 |
| CviQI | GTAC | 2 cut(s) | 522, 762 |
| DpnI | GATC | 6 cut(s) | 44, 62, 93, 593, 645, 729 |
| DpnII | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| DriI | GACNNNNNGTC | 1 cut(s) | 498 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 498 |
| EciI | GGCGGA | 1 cut(s) | 792 |
| Eco24I | GRGCYC | 1 cut(s) | 576 |
| Eco47I | GGWCC | 3 cut(s) | 205, 291, 516 |
| EcoO109I | RGGNCCY | 1 cut(s) | 516 |
| EcoRI | GAATTC | 2 cut(s) | 258, 700 |
| EcoT22I | ATGCAT | 1 cut(s) | 742 |
| EcoT38I | GRGCYC | 1 cut(s) | 576 |
| FaeI | CATG | 4 cut(s) | 241, 403, 750, 806 |
| FaqI | GGGAC | 2 cut(s) | 209, 529 |
| FatI | CATG | 4 cut(s) | 237, 399, 746, 802 |
| FbaI | TGATCA | 1 cut(s) | 91 |
| FblI | GTMKAC | 1 cut(s) | 606 |
| Fnu4HI | GCNGC | 3 cut(s) | 112, 115, 558 |
| FokI | GGATG | 1 cut(s) | 727 |
| FriOI | GRGCYC | 1 cut(s) | 576 |
| Fsp4HI | GCNGC | 3 cut(s) | 112, 115, 558 |
| FspBI | CTAG | 4 cut(s) | 177, 215, 306, 759 |
| FspI | TGCGCA | 1 cut(s) | 724 |
| GlaI | GCGC | 2 cut(s) | 247, 724 |
| GluI | GCNGC | 3 cut(s) | 112, 115, 558 |
| HapII | CCGG | 2 cut(s) | 195, 576 |
| HgaI | GACGC | 1 cut(s) | 124 |
| HhaI | GCGC | 2 cut(s) | 248, 725 |
| Hin1I | GRCGYC | 2 cut(s) | 135, 222 |
| Hin1II | CATG | 4 cut(s) | 241, 403, 750, 806 |
| Hin6I | GCGC | 2 cut(s) | 246, 723 |
| HinP1I | GCGC | 2 cut(s) | 246, 723 |
| HincII | GTYRAC | 2 cut(s) | 349, 784 |
| HindII | GTYRAC | 2 cut(s) | 349, 784 |
| HinfI | GANTC | 3 cut(s) | 665, 708, 785 |
| HpaII | CCGG | 2 cut(s) | 195, 576 |
| HphI | GGTGA | 2 cut(s) | 502, 586 |
| Hpy166II | GTNNAC | 4 cut(s) | 169, 349, 607, 784 |
| Hpy188I | TCNGA | 2 cut(s) | 140, 339 |
| Hpy188III | TCNNGA | 2 cut(s) | 669, 705 |
| Hpy8I | GTNNAC | 4 cut(s) | 169, 349, 607, 784 |
| Hpy99I | CGWCG | 2 cut(s) | 140, 224 |
| HpyAV | CCTTC | 1 cut(s) | 20 |
| HpyCH4III | ACNGT | 4 cut(s) | 185, 295, 496, 678 |
| HpyCH4IV | ACGT | 4 cut(s) | 222, 404, 434, 484 |
| HpyCH4V | TGCA | 8 cut(s) | 4, 111, 265, 456, 625, 631, 740, 806 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 141, 462, 563, 731 |
| HpySE526I | ACGT | 4 cut(s) | 222, 404, 434, 484 |
| Hsp92I | GRCGYC | 2 cut(s) | 135, 222 |
| Hsp92II | CATG | 4 cut(s) | 241, 403, 750, 806 |
| HspAI | GCGC | 2 cut(s) | 246, 723 |
| Ksp22I | TGATCA | 1 cut(s) | 91 |
| Kzo9I | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| LmnI | GCTCC | 1 cut(s) | 21 |
| Lsp1109I | GCAGC | 3 cut(s) | 123, 126, 569 |
| LweI | GCATC | 2 cut(s) | 589, 749 |
| MaeI | CTAG | 4 cut(s) | 177, 215, 306, 759 |
| MaeII | ACGT | 4 cut(s) | 222, 404, 434, 484 |
| MaeIII | GTNAC | 2 cut(s) | 179, 490 |
| MalI | GATC | 6 cut(s) | 44, 62, 93, 593, 645, 729 |
| MboI | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| MfeI | CAATTG | 2 cut(s) | 124, 626 |
| MhlI | GDGCHC | 1 cut(s) | 576 |
| MluCI | AATT | 7 cut(s) | 98, 124, 258, 283, 626, 700, 807 |
| MlyI | GAGTC | 1 cut(s) | 779 |
| MmeI | TCCRAC | 2 cut(s) | 118, 362 |
| MnlI | CCTC | 5 cut(s) | 134, 196, 368, 523, 665 |
| Mph1103I | ATGCAT | 1 cut(s) | 742 |
| MseI | TTAA | 5 cut(s) | 297, 636, 687, 810, 820 |
| MslI | CAYNNNNRTG | 1 cut(s) | 745 |
| MspI | CCGG | 2 cut(s) | 195, 576 |
| MspR9I | CCNGG | 1 cut(s) | 576 |
| MunI | CAATTG | 2 cut(s) | 124, 626 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 141, 462, 563, 731 |
| NciI | CCSGG | 1 cut(s) | 576 |
| NdeII | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| NlaIII | CATG | 4 cut(s) | 241, 403, 750, 806 |
| NlaIV | GGNNCC | 1 cut(s) | 517 |
| NmuCI | GTSAC | 1 cut(s) | 490 |
| NsbI | TGCGCA | 1 cut(s) | 724 |
| NsiI | ATGCAT | 1 cut(s) | 742 |
| NspI | RCATGY | 2 cut(s) | 750, 806 |
| PaeI | GCATGC | 1 cut(s) | 806 |
| PciI | ACATGT | 1 cut(s) | 746 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 648 |
| PfeI | GAWTC | 2 cut(s) | 665, 708 |
| PflFI | GACNNNGTC | 1 cut(s) | 221 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 512 |
| PkrI | GCNGC | 3 cut(s) | 113, 116, 559 |
| PleI | GAGTC | 1 cut(s) | 779 |
| PpsI | GAGTC | 1 cut(s) | 779 |
| PpuMI | RGGWCCY | 1 cut(s) | 516 |
| PscI | ACATGT | 1 cut(s) | 746 |
| PshAI | GACNNNNGTC | 1 cut(s) | 344 |
| PshBI | ATTAAT | 1 cut(s) | 810 |
| Psp1406I | AACGTT | 1 cut(s) | 434 |
| Psp5II | RGGWCCY | 1 cut(s) | 516 |
| PspN4I | GGNNCC | 1 cut(s) | 517 |
| PspPI | GGNCC | 3 cut(s) | 205, 291, 516 |
| PspPPI | RGGWCCY | 1 cut(s) | 516 |
| PstI | CTGCAG | 1 cut(s) | 113 |
| PstNI | CAGNNNCTG | 1 cut(s) | 330 |
| PsyI | GACNNNGTC | 1 cut(s) | 221 |
| RsaI | GTAC | 2 cut(s) | 523, 763 |
| RsaNI | GTAC | 2 cut(s) | 522, 762 |
| RseI | CAYNNNNRTG | 1 cut(s) | 745 |
| SaqAI | TTAA | 5 cut(s) | 297, 636, 687, 810, 820 |
| SatI | GCNGC | 3 cut(s) | 112, 115, 558 |
| Sau3AI | GATC | 6 cut(s) | 42, 60, 91, 591, 643, 727 |
| Sau96I | GGNCC | 3 cut(s) | 205, 291, 516 |
| ScaI | AGTACT | 1 cut(s) | 763 |
| SchI | GAGTC | 1 cut(s) | 779 |
| ScrFI | CCNGG | 1 cut(s) | 576 |
| SduI | GDGCHC | 1 cut(s) | 576 |
| SfaNI | GCATC | 2 cut(s) | 589, 749 |
| SfcI | CTRYAG | 3 cut(s) | 109, 253, 567 |
| SinI | GGWCC | 3 cut(s) | 205, 291, 516 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 745 |
| SpeI | ACTAGT | 1 cut(s) | 758 |
| SphI | GCATGC | 1 cut(s) | 806 |
| Sse9I | AATT | 7 cut(s) | 98, 124, 258, 283, 626, 700, 807 |
| SsiI | CCGC | 2 cut(s) | 602, 777 |
| SspMI | CTAG | 4 cut(s) | 177, 215, 306, 759 |
| StyD4I | CCNGG | 1 cut(s) | 574 |
| TaaI | ACNGT | 4 cut(s) | 185, 295, 496, 678 |
| TaiI | ACGT | 4 cut(s) | 225, 407, 437, 487 |
| TaqI | TCGA | 2 cut(s) | 59, 69 |
| TasI | AATT | 7 cut(s) | 98, 124, 258, 283, 626, 700, 807 |
| TatI | WGTACW | 2 cut(s) | 521, 761 |
| TfiI | GAWTC | 2 cut(s) | 665, 708 |
| Tru1I | TTAA | 5 cut(s) | 297, 636, 687, 810, 820 |
| Tru9I | TTAA | 5 cut(s) | 297, 636, 687, 810, 820 |
| TscAI | CASTG | 3 cut(s) | 331, 627, 683 |
| TseFI | GTSAC | 1 cut(s) | 490 |
| TseI | GCWGC | 3 cut(s) | 111, 114, 557 |
| Tsp45I | GTSAC | 1 cut(s) | 490 |
| TspDTI | ATGAA | 1 cut(s) | 284 |
| TspRI | CASTG | 3 cut(s) | 331, 627, 683 |
| Tth111I | GACNNNGTC | 1 cut(s) | 221 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 512 |
| VpaK11BI | GGWCC | 3 cut(s) | 205, 291, 516 |
| VspI | ATTAAT | 1 cut(s) | 810 |
| XapI | RAATTY | 3 cut(s) | 258, 283, 700 |
| XceI | RCATGY | 2 cut(s) | 750, 806 |
| XmiI | GTMKAC | 1 cut(s) | 606 |
| XspI | CTAG | 4 cut(s) | 177, 215, 306, 759 |
| ZraI | GACGTC | 1 cut(s) | 223 |
| ZrmI | AGTACT | 1 cut(s) | 763 |
| Zsp2I | ATGCAT | 1 cut(s) | 742 |