Prupe.4G025600_v2.0.a1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0009539
Physical Location & Seq
Forward (+)
1206048 .. 1208570
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G025600.1

Sequence Viewer

Length: 903 bp
ATGGAAAGAATGGCATCTCTGATGCCTATTCTTCTTTACATTGCAGTACTCATTGCTGCTTGCTCTTCTTCTTCATCTGAAGCGGCTGCCCCAGCCACTTCTTTCAAAGACAATTTCGACATAATGTGGTCTGAGAACCATTTTAAAACCTCTGCAGATGGGGAGATCTGGTATCTTTCCCTGGACAAAGAAACAGGTTGCGGGTTTCAGACAAAACAGAAGTACAGATTTGGATGGTTCAGCATGAAGCTCAAGTTGGTTGGAGGTGACTCTGCTGGTGTAGTGACAGCTTATTATATGTGCACAGAAAATGGAGCAGGGCCAGAGAGGGATGAGTTAGATTTTGAGTTCTTGGGAAATAGGAGCGGGCAACCTTATCTCATTCAGACAAATGTTTACAAGAACGGAACTGGAGGCCGTGAGATGAGGCACATGCTGTGGTTCGACCCCACCGAGGACTTTCATACCTATTCAATTCTTTGGAACAATCATCAGATTGTGTTTTTTGTGGACAAAGTTCCCATAAGGGTGTTCAAGAACAATGGGGAAGCAAACAACTTCTTCCCCAATGAGAAGCCAATGTACTTGTTCTCCAGCATATGGAATGCAGATGAGTGGGCAACCAGAGGTGGGCTTGAGAAGACAGACTGGAAGAAAGCCCCATTTGTGTCCTCTTACAAAGACTTCAATGTTGATGGGTGCCAATGGGAAGACCCTTACCCTGCATGCGTCTCCACCACAACAAAGAATTGGTGGGACCAGTATGATGCTTGGCACCTCTCTGATGCTCAGAAAATGGACTATGCTTGGATACAGAGAAATCTCGTTATTTATGATTATTGCAAGGATTCTGAACGCTACCCAACTTTGCCAGTGGAGTGCCCGTTGAGTCCATGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

301

Amino Acids

34.77

Weight (kDa)

4.94

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 699, 774
AccB7I CCANNNNNTGG 1 cut(s) 600
AccBSI CCGCTC 1 cut(s) 366
AciI CCGC 3 cut(s) 83, 201, 366
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 3 cut(s) 48, 224, 584
AfiI CCNNNNNNNGG 3 cut(s) 454, 600, 630
AgsI TTSAA 4 cut(s) 106, 474, 535, 688
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 2 cut(s) 250, 290
AluI AGCT 2 cut(s) 250, 290
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 736
Alw44I GTGCAC 1 cut(s) 301
AoxI GGCC 2 cut(s) 320, 415
ApaLI GTGCAC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 56, 86
AspS9I GGNCC 2 cut(s) 320, 757
AsuHPI GGTGA 1 cut(s) 278
AvaII GGWCC 1 cut(s) 757
BaeGI GKGCMC 2 cut(s) 305, 884
BanI GGYRCC 2 cut(s) 699, 774
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 2 cut(s) 647, 717
Bbv12I GWGCWC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 43, 73
BccI CCATC 3 cut(s) 152, 228, 689
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 182
BciVI GTATCC 1 cut(s) 804
BcoDI GTCTC 1 cut(s) 736
BfmI CTRYAG 1 cut(s) 153
BfuI GTATCC 1 cut(s) 804
BglII AGATCT 1 cut(s) 165
BisI GCNGC 3 cut(s) 57, 84, 87
BlsI GCNGC 3 cut(s) 58, 85, 88
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 757
BmgT120I GGNCC 2 cut(s) 320, 757
BmiI GGNNCC 3 cut(s) 701, 758, 776
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 4 cut(s) 12, 23, 757, 775
BpiI GAAGAC 2 cut(s) 647, 717
BpmI CTGGAG 2 cut(s) 432, 577
BpuEI CTTGAG 2 cut(s) 236, 656
BsaJI CCNNGG 3 cut(s) 180, 453, 893
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 575, 605, 869, 899
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 600, 630
Bse1I ACTGG 4 cut(s) 415, 653, 760, 872
Bse3DI GCAATG 2 cut(s) 39, 51
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 3 cut(s) 180, 453, 893
BseGI GGATG 2 cut(s) 239, 337
BseLI CCNNNNNNNGG 3 cut(s) 454, 600, 630
BseMI GCAATG 2 cut(s) 39, 51
BseMII CTCAG 2 cut(s) 123, 803
BseNI ACTGG 4 cut(s) 415, 653, 760, 872
BseSI GKGCMC 2 cut(s) 305, 884
BseXI GCAGC 2 cut(s) 43, 73
BseYI CCCAGC 1 cut(s) 91
BshFI GGCC 2 cut(s) 322, 417
BshNI GGYRCC 2 cut(s) 699, 774
BsiHKAI GWGCWC 1 cut(s) 305
BslFI GGGAC 1 cut(s) 770
BslI CCNNNNNNNGG 3 cut(s) 454, 600, 630
BsmAI GTCTC 1 cut(s) 736
BsmBI CGTCTC 1 cut(s) 736
BsmFI GGGAC 1 cut(s) 770
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 2 cut(s) 322, 417
Bsp1286I GDGCHC 2 cut(s) 305, 884
Bsp143I GATC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 893
BspACI CCGC 3 cut(s) 83, 201, 366
BspANI GGCC 2 cut(s) 322, 417
BspCNI CTCAG 2 cut(s) 124, 802
BspLI GGNNCC 3 cut(s) 701, 758, 776
BspMAI CTGCAG 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 70
BspT107I GGYRCC 2 cut(s) 699, 774
BsrBI CCGCTC 1 cut(s) 366
BsrDI GCAATG 2 cut(s) 39, 51
BsrI ACTGG 4 cut(s) 415, 653, 760, 872
BssECI CCNNGG 3 cut(s) 180, 453, 893
BssMI GATC 1 cut(s) 165
BssT1I CCWWGG 1 cut(s) 893
Bst2UI CCWGG 1 cut(s) 182
Bst6I CTCTTC 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 61, 368, 727
BstDEI CTNAG 2 cut(s) 132, 789
BstDSI CCRYGG 1 cut(s) 893
BstF5I GGATG 2 cut(s) 239, 337
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 1 cut(s) 165
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 2 cut(s) 436, 729
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 2 cut(s) 305, 884
BstV1I GCAGC 2 cut(s) 43, 73
BstV2I GAAGAC 2 cut(s) 647, 717
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 804
BsuRI GGCC 2 cut(s) 322, 417
BtgI CCRYGG 1 cut(s) 893
BtsCI GGATG 2 cut(s) 239, 337
BtsIMutI CAGTG 1 cut(s) 879
Cac8I GCNNGC 3 cut(s) 61, 368, 727
Cfr13I GGNCC 2 cut(s) 320, 757
CseI GACGC 1 cut(s) 718
Csp6I GTAC 3 cut(s) 47, 223, 583
CviAII CATG 4 cut(s) 244, 433, 726, 894
CviJI RGCY 9 cut(s) 86, 95, 250, 290, 322, 417, 577, 634, 659
CviKI_1 RGCY 9 cut(s) 86, 95, 250, 290, 322, 417, 577, 634, 659
CviQI GTAC 3 cut(s) 47, 223, 583
DdeI CTNAG 2 cut(s) 132, 789
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
DraI TTTAAA 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 893
Eco47I GGWCC 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 893
ErhI CCWWGG 1 cut(s) 893
Esp3I CGTCTC 1 cut(s) 736
FaeI CATG 4 cut(s) 247, 436, 729, 897
FaqI GGGAC 1 cut(s) 770
FatI CATG 4 cut(s) 243, 432, 725, 893
FauI CCCGC 2 cut(s) 194, 359
FauNDI CATATG 1 cut(s) 599
Fnu4HI GCNGC 3 cut(s) 57, 84, 87
FokI GGATG 2 cut(s) 246, 344
Fsp4HI GCNGC 3 cut(s) 57, 84, 87
GluI GCNGC 3 cut(s) 57, 84, 87
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 2 cut(s) 432, 577
HaeIII GGCC 2 cut(s) 322, 417
HgaI GACGC 1 cut(s) 718
Hin1II CATG 4 cut(s) 247, 436, 729, 897
HinfI GANTC 3 cut(s) 269, 848, 889
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 3 cut(s) 303, 397, 511
Hpy188I TCNGA 9 cut(s) 21, 79, 133, 210, 387, 495, 784, 792, 853
Hpy188III TCNNGA 1 cut(s) 535
Hpy8I GTNNAC 3 cut(s) 303, 397, 511
HpyCH4V TGCA 6 cut(s) 44, 155, 303, 608, 725, 843
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 2 cut(s) 132, 789
Hsp92II CATG 4 cut(s) 247, 436, 729, 897
Kzo9I GATC 1 cut(s) 165
LguI GCTCTTC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 314, 363
Lsp1109I GCAGC 2 cut(s) 43, 73
LweI GCATC 4 cut(s) 12, 23, 757, 775
MaeIII GTNAC 2 cut(s) 266, 283
MalI GATC 1 cut(s) 167
MbiI CCGCTC 1 cut(s) 366
MboI GATC 1 cut(s) 165
MboII GAAGA 8 cut(s) 23, 57, 60, 63, 553, 652, 664, 722
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 305, 884
MluCI AATT 3 cut(s) 112, 474, 748
MlyI GAGTC 2 cut(s) 263, 898
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 9 cut(s) 160, 257, 321, 407, 420, 448, 620, 682, 788
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 2 cut(s) 527, 898
MspR9I CCNGG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 92
NcoI CCATGG 1 cut(s) 893
NdeI CATATG 1 cut(s) 599
NdeII GATC 1 cut(s) 165
NlaIII CATG 4 cut(s) 247, 436, 729, 897
NlaIV GGNNCC 3 cut(s) 701, 758, 776
NmuCI GTSAC 2 cut(s) 266, 283
NspI RCATGY 2 cut(s) 436, 729
PaeI GCATGC 1 cut(s) 729
PciSI GCTCTTC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 1 cut(s) 610
PfeI GAWTC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 600
PkrI GCNGC 3 cut(s) 58, 85, 88
PleI GAGTC 2 cut(s) 263, 897
PpsI GAGTC 2 cut(s) 263, 897
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 3 cut(s) 701, 758, 776
PspPI GGNCC 2 cut(s) 320, 757
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 165
RsaI GTAC 3 cut(s) 48, 224, 584
RsaNI GTAC 3 cut(s) 47, 223, 583
RseI CAYNNNNRTG 2 cut(s) 527, 898
SapI GCTCTTC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 57, 84, 87
Sau3AI GATC 1 cut(s) 165
Sau96I GGNCC 2 cut(s) 320, 757
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 2 cut(s) 263, 898
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 2 cut(s) 305, 884
SetI ASST 9 cut(s) 152, 199, 252, 268, 292, 376, 470, 631, 780
SfaNI GCATC 4 cut(s) 12, 23, 757, 775
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 757
SmiMI CAYNNNNRTG 2 cut(s) 527, 898
SmlI CTYRAG 2 cut(s) 251, 635
SmoI CTYRAG 2 cut(s) 251, 635
SphI GCATGC 1 cut(s) 729
Sse9I AATT 3 cut(s) 112, 474, 748
SsiI CCGC 3 cut(s) 83, 201, 366
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 893
TaqI TCGA 2 cut(s) 117, 444
TasI AATT 3 cut(s) 112, 474, 748
TatI WGTACW 3 cut(s) 46, 222, 582
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 2 cut(s) 266, 283
TseI GCWGC 2 cut(s) 56, 86
Tsp45I GTSAC 2 cut(s) 266, 283
TspDTI ATGAA 3 cut(s) 63, 260, 452
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 1 cut(s) 879
Van91I CCANNNNNTGG 1 cut(s) 600
VneI GTGCAC 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 757
XceI RCATGY 2 cut(s) 436, 729
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.