RLG00000031188

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009539
Physical Location & Seq
Forward (+)
2791884 .. 2792303
420 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031188

Sequence Viewer

Length: 420 bp
ATGGATTCTGCTGGCAGTTTTTTTGTTGACAAAGTTCCCATAAGGGTGTTTAAGAACAATGGTGAAGCAAACGACTTCTTCCCCAACGAGAAACCCATGTATTTGTTCTCGAGCATATGGAATGCCGATGAATGGGCGACGAGAGGTGGACTTGAGAAGACAGACTGGAAAAAATCACCATTTGTGTCTTCCTACAAGGACTTCAGTGTTGATGCATGCCAGTGGGAAGATCCATACCCTAAATGTGTGTCCACAACAACAGAGAACTGGTGGGATCAGTATGATGCTTGGCACCTTTCAGATTCTCAGAAAATGGACTATGCTTGGATACAAAGAAACCTTGTTGTTTATGATTATTGCAAGGACACGGAGAGGTTCCCAACATTGCCAGTGGAGTGTCCATTGAGTCCATGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

140

Amino Acids

16.46

Weight (kDa)

4.54

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 6 - 58 1.6e-12 Glycosyl hydrolases family 16
XET_C PF06955 86 - 133 3.1e-16 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 291
AclWI GGATC 2 cut(s) 224, 282
AcuI CTGAAG 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 132
AlwI GGATC 2 cut(s) 224, 282
Ama87I CYCGRG 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 74, 168
AvaI CYCGRG 1 cut(s) 109
BanI GGYRCC 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 164, 180
BciVI GTATCC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 293, 377
BmsI GCATC 2 cut(s) 202, 274
BpiI GAAGAC 2 cut(s) 164, 180
BpuEI CTTGAG 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 410
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 4 cut(s) 170, 220, 272, 389
Bse3DI GCAATG 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMI GCAATG 1 cut(s) 383
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 4 cut(s) 170, 220, 272, 389
BshNI GGYRCC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 132
BsmI GAATGC 1 cut(s) 127
BsoBI CYCGRG 1 cut(s) 109
Bsp143I GATC 2 cut(s) 229, 274
Bsp19I CCATGG 1 cut(s) 410
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 293, 377
BspPI GGATC 2 cut(s) 224, 282
BspT107I GGYRCC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 383
BsrI ACTGG 4 cut(s) 170, 220, 272, 389
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 2 cut(s) 229, 274
BssT1I CCWWGG 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 13, 217
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 410
BstKTI GATC 2 cut(s) 232, 277
BstMBI GATC 2 cut(s) 229, 274
BstNSI RCATGY 1 cut(s) 219
BstV2I GAAGAC 2 cut(s) 164, 180
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuI GTATCC 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 410
BtsIMutI CAGTG 3 cut(s) 211, 227, 396
Cac8I GCNNGC 2 cut(s) 13, 217
CviAII CATG 3 cut(s) 97, 216, 411
DdeI CTNAG 1 cut(s) 306
DpnI GATC 2 cut(s) 231, 276
DpnII GATC 2 cut(s) 229, 274
Eco130I CCWWGG 1 cut(s) 410
Eco57I CTGAAG 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 410
EcoT22I ATGCAT 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 410
FaeI CATG 3 cut(s) 100, 219, 414
FatI CATG 3 cut(s) 96, 215, 410
FauNDI CATATG 1 cut(s) 116
Hin1II CATG 3 cut(s) 100, 219, 414
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 3 cut(s) 5, 302, 406
HphI GGTGA 2 cut(s) 74, 168
Hpy166II GTNNAC 3 cut(s) 28, 149, 252
Hpy188I TCNGA 2 cut(s) 301, 309
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 3 cut(s) 28, 149, 252
Hpy99I CGWCG 1 cut(s) 142
HpyCH4V TGCA 2 cut(s) 215, 360
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 3 cut(s) 100, 219, 414
Kzo9I GATC 2 cut(s) 229, 274
LpnPI CCDG 4 cut(s) 151, 233, 253, 402
LweI GCATC 2 cut(s) 202, 274
MalI GATC 2 cut(s) 231, 276
MboI GATC 2 cut(s) 229, 274
MboII GAAGA 4 cut(s) 70, 169, 180, 239
MflI RGATCY 1 cut(s) 229
MlyI GAGTC 1 cut(s) 415
MnlI CCTC 2 cut(s) 137, 366
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 3 cut(s) 44, 220, 415
Mva1269I GAATGC 1 cut(s) 127
NcoI CCATGG 1 cut(s) 410
NdeI CATATG 1 cut(s) 116
NdeII GATC 2 cut(s) 229, 274
NlaIII CATG 3 cut(s) 100, 219, 414
NlaIV GGNNCC 2 cut(s) 293, 377
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 1 cut(s) 219
PaeI GCATGC 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 109
PctI GAATGC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 5, 302
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 293, 377
PsuI RGATCY 1 cut(s) 229
RseI CAYNNNNRTG 3 cut(s) 44, 220, 415
SaqAI TTAA 1 cut(s) 51
Sau3AI GATC 2 cut(s) 229, 274
SchI GAGTC 1 cut(s) 415
SetI ASST 4 cut(s) 148, 297, 342, 377
SfaNI GCATC 2 cut(s) 202, 274
Sfr274I CTCGAG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 109
SmiMI CAYNNNNRTG 3 cut(s) 44, 220, 415
SmlI CTYRAG 2 cut(s) 109, 152
SmoI CTYRAG 2 cut(s) 109, 152
SphI GCATGC 1 cut(s) 219
StyI CCWWGG 1 cut(s) 410
TaqI TCGA 1 cut(s) 110
TfiI GAWTC 2 cut(s) 5, 302
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 3 cut(s) 211, 227, 396
TspDTI ATGAA 1 cut(s) 144
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 3 cut(s) 211, 227, 396
XceI RCATGY 1 cut(s) 219
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XhoI CTCGAG 1 cut(s) 109
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.