Prupe.4G058000_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0009088
Physical Location & Seq
Reverse (-)
2770791 .. 2775957
5167 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G058000.2

Sequence Viewer

Length: 717 bp
ATGAGGCCAAAGATTTACCTTTTTGGTGACTCAATCACCGAGGAGTCTTTCGGTGATGGTGGCTGGGGTGCCTCTCTTGCCCATCATTTCTCTCGCACGGTTGACGTGGTGCTAAGAGGGTACAGTGGCTACAACACCAGGTGGGCTCTGCAAGTTTTGGACAGGGTCTTTCCGAGCAGTGAAAACAGAGAAGATGCTCCACTAGCTGTCACCGTCTTCTTTGGCGCTAATGACGCTTGCCTCCCCGATAGGTGCTCTGCTTTTCAACACGTGCCTGTCAACGAGTACAAGCATAATCTCCACTCCATTGTCTCCTTTCTCAAGAAGCGATGGCCTATGACTCGCATTCTCCTTATCACCCCTCCTCCAATCGATGAAGATGGGCGCCTTCGGCATCCCTATATTGATAATCCATCCAATCTGCCTGAGAGGACAAATAAAGCTGCTGGTGCTTATTCAGAGGCATGTGTTGCTGTAGCTCGAGAAAGTGGGAACCCAGTAATAGATCTTTGGACTAAAATGCAGCAGTTTCCTGACTGGGAAAAAGCTCATCTAAGGGATGGCTTGCACCTTACTCTGCGGGGGAACATGGTTGTGATTGAGGAGGTGGTTCTGAAGCTCAAGAATGAAGGCTTGAGTCTAGAATCTTTGCCAGTTGATCTTCCACTTATAAATAACATAGACCCTAAAGACCCTCTTAAGGCTTTTCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

239

Amino Acids

26.84

Weight (kDa)

6.06

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AccB1I GGYRCC 2 cut(s) 68, 384
AciI CCGC 1 cut(s) 580
AcuI CTGAAG 1 cut(s) 635
AcvI CACGTG 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 385
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 2 cut(s) 122, 287
AfiI CCNNNNNNNGG 1 cut(s) 700
AflII CTTAAG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 2 cut(s) 266, 710
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 5 cut(s) 206, 443, 479, 548, 619
AluI AGCT 5 cut(s) 206, 443, 479, 548, 619
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 2 cut(s) 5, 332
ApeKI GCWGC 2 cut(s) 443, 523
AspLEI GCGC 2 cut(s) 227, 387
AsuHPI GGTGA 5 cut(s) 28, 38, 65, 202, 349
AvaI CYCGRG 1 cut(s) 480
BanI GGYRCC 2 cut(s) 68, 384
BanII GRGCYC 1 cut(s) 148
BbrPI CACGTG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 2 cut(s) 430, 535
BccI CCATC 6 cut(s) 50, 90, 324, 374, 421, 554
BciT130I CCWGG 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 2 cut(s) 203, 641
BfmI CTRYAG 1 cut(s) 474
BfoI RGCGCY 2 cut(s) 228, 388
BfrI CTTAAG 1 cut(s) 698
BglII AGATCT 1 cut(s) 505
BisI GCNGC 2 cut(s) 444, 524
BlsI GCNGC 2 cut(s) 445, 525
Bme1390I CCNGG 1 cut(s) 139
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 70, 386, 494
BmrFI CCNGG 1 cut(s) 139
BmrI ACTGGG 2 cut(s) 491, 547
BmsI GCATC 2 cut(s) 184, 403
BmuI ACTGGG 2 cut(s) 491, 547
BpiI GAAGAC 1 cut(s) 208
BpuEI CTTGAG 3 cut(s) 305, 605, 655
Bsa29I ATCGAT 1 cut(s) 372
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 1 cut(s) 385
BsaJI CCNNGG 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse1I ACTGG 3 cut(s) 497, 542, 653
BseBI CCWGG 1 cut(s) 139
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 3 cut(s) 394, 413, 565
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 3 cut(s) 497, 542, 653
BseRI GAGGAG 3 cut(s) 56, 354, 617
BseXI GCAGC 2 cut(s) 430, 535
BseYI CCCAGC 1 cut(s) 63
BshFI GGCC 2 cut(s) 7, 334
BshNI GGYRCC 2 cut(s) 68, 384
BshVI ATCGAT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 2 cut(s) 7, 334
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 148, 257
Bsp143I GATC 2 cut(s) 505, 658
BspACI CCGC 1 cut(s) 580
BspANI GGCC 2 cut(s) 7, 334
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 372
BspLI GGNNCC 3 cut(s) 70, 386, 494
BspT107I GGYRCC 2 cut(s) 68, 384
BspTI CTTAAG 1 cut(s) 698
BsrI ACTGG 3 cut(s) 497, 542, 653
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 505, 658
BssNI GRCGYC 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 3 cut(s) 100, 125, 214
BstACI GRCGYC 1 cut(s) 385
BstAFI CTTAAG 1 cut(s) 698
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 238, 566
BstDEI CTNAG 3 cut(s) 113, 426, 554
BstF5I GGATG 3 cut(s) 394, 413, 565
BstH2I RGCGCY 2 cut(s) 228, 388
BstHHI GCGC 2 cut(s) 227, 387
BstKTI GATC 2 cut(s) 508, 661
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 2 cut(s) 505, 658
BstMWI GCNNNNNNNGC 6 cut(s) 77, 203, 233, 391, 449, 470
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 2 cut(s) 430, 535
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 372
BsuRI GGCC 2 cut(s) 7, 334
BsuTUI ATCGAT 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 343
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 3 cut(s) 394, 413, 565
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 130, 184
Cac8I GCNNGC 2 cut(s) 238, 566
CfoI GCGC 2 cut(s) 227, 387
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 1 cut(s) 242
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 121, 286
CviAII CATG 2 cut(s) 465, 589
CviQI GTAC 2 cut(s) 121, 286
DdeI CTNAG 3 cut(s) 113, 426, 554
DinI GGCGCC 1 cut(s) 386
DpnI GATC 2 cut(s) 507, 660
DpnII GATC 2 cut(s) 505, 658
DraIII CACNNNGTG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 635
Eco72I CACGTG 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 148
EgeI GGCGCC 1 cut(s) 386
EheI GGCGCC 1 cut(s) 386
FaeI CATG 2 cut(s) 468, 592
FaiI YATR 7 cut(s) 294, 338, 402, 466, 590, 671, 680
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FatI CATG 2 cut(s) 464, 588
FauI CCCGC 1 cut(s) 573
Fnu4HI GCNGC 2 cut(s) 444, 524
FokI GGATG 3 cut(s) 381, 400, 572
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 2 cut(s) 444, 524
FspBI CTAG 2 cut(s) 203, 641
GlaI GCGC 2 cut(s) 226, 386
GluI GCNGC 2 cut(s) 444, 524
GsaI CCCAGC 1 cut(s) 67
HaeII RGCGCY 2 cut(s) 228, 388
HaeIII GGCC 2 cut(s) 7, 334
HgaI GACGC 1 cut(s) 242
HhaI GCGC 2 cut(s) 227, 387
Hin1I GRCGYC 1 cut(s) 385
Hin1II CATG 2 cut(s) 468, 592
Hin6I GCGC 2 cut(s) 225, 385
HinP1I GCGC 2 cut(s) 225, 385
HincII GTYRAC 2 cut(s) 103, 280
HindII GTYRAC 2 cut(s) 103, 280
HinfI GANTC 5 cut(s) 29, 44, 340, 637, 644
HphI GGTGA 5 cut(s) 28, 38, 65, 202, 349
Hpy166II GTNNAC 2 cut(s) 103, 280
Hpy188I TCNGA 3 cut(s) 174, 460, 615
Hpy188III TCNNGA 5 cut(s) 322, 482, 533, 622, 641
Hpy8I GTNNAC 2 cut(s) 103, 280
HpyAV CCTTC 2 cut(s) 398, 623
HpyCH4III ACNGT 3 cut(s) 100, 125, 214
HpyCH4IV ACGT 2 cut(s) 105, 270
HpyCH4V TGCA 3 cut(s) 151, 523, 568
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 203, 233, 391, 449, 470
HpyF3I CTNAG 3 cut(s) 113, 426, 554
HpySE526I ACGT 2 cut(s) 105, 270
Hsp92I GRCGYC 1 cut(s) 385
Hsp92II CATG 2 cut(s) 468, 592
HspAI GCGC 2 cut(s) 225, 385
KasI GGCGCC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 505, 658
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 2 cut(s) 430, 535
LweI GCATC 2 cut(s) 184, 403
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 2 cut(s) 203, 641
MaeII ACGT 2 cut(s) 105, 270
MaeIII GTNAC 2 cut(s) 26, 208
MalI GATC 2 cut(s) 507, 660
MboI GATC 2 cut(s) 505, 658
MboII GAAGA 4 cut(s) 203, 208, 389, 653
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 148, 257
MluCI AATT 1 cut(s) 712
Mly113I GGCGCC 1 cut(s) 385
MlyI GAGTC 4 cut(s) 23, 53, 334, 646
MseI TTAA 1 cut(s) 699
MslI CAYNNNNRTG 1 cut(s) 593
MspCI CTTAAG 1 cut(s) 698
MspR9I CCNGG 1 cut(s) 139
Mva1269I GAATGC 1 cut(s) 345
MvaI CCWGG 1 cut(s) 139
MwoI GCNNNNNNNGC 6 cut(s) 77, 203, 233, 391, 449, 470
NarI GGCGCC 1 cut(s) 385
NdeII GATC 2 cut(s) 505, 658
NlaIII CATG 2 cut(s) 468, 592
NlaIV GGNNCC 3 cut(s) 70, 386, 494
NmuCI GTSAC 2 cut(s) 26, 208
NspI RCATGY 1 cut(s) 468
PaeR7I CTCGAG 1 cut(s) 480
PctI GAATGC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 644
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 445, 525
PleI GAGTC 4 cut(s) 23, 52, 334, 645
PluTI GGCGCC 1 cut(s) 388
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 4 cut(s) 23, 52, 334, 645
Ppu21I YACGTR 1 cut(s) 271
PsiI TTATAA 1 cut(s) 671
Psp6I CCWGG 1 cut(s) 137
PspCI CACGTG 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 3 cut(s) 70, 386, 494
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 2 cut(s) 122, 287
RsaNI GTAC 2 cut(s) 121, 286
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 1 cut(s) 699
SatI GCNGC 2 cut(s) 444, 524
Sau3AI GATC 2 cut(s) 505, 658
SchI GAGTC 4 cut(s) 23, 53, 334, 646
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 148, 257
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 2 cut(s) 184, 403
SfcI CTRYAG 1 cut(s) 474
SfoI GGCGCC 1 cut(s) 386
Sfr274I CTCGAG 1 cut(s) 480
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 5 cut(s) 320, 480, 620, 634, 698
SmoI CTYRAG 5 cut(s) 320, 480, 620, 634, 698
Sse9I AATT 1 cut(s) 712
SsiI CCGC 1 cut(s) 580
SspDI GGCGCC 1 cut(s) 384
SspMI CTAG 2 cut(s) 203, 641
StyD4I CCNGG 1 cut(s) 137
TaaI ACNGT 3 cut(s) 100, 125, 214
TaiI ACGT 2 cut(s) 108, 273
TaqI TCGA 2 cut(s) 372, 481
TasI AATT 1 cut(s) 712
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 1 cut(s) 644
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TscAI CASTG 2 cut(s) 130, 184
TseFI GTSAC 2 cut(s) 26, 208
TseI GCWGC 2 cut(s) 443, 523
Tsp45I GTSAC 2 cut(s) 26, 208
TspDTI ATGAA 2 cut(s) 390, 642
TspRI CASTG 2 cut(s) 130, 184
Tth111I GACNNNGTC 1 cut(s) 164
Vha464I CTTAAG 1 cut(s) 698
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 1 cut(s) 468
XhoI CTCGAG 1 cut(s) 480
XspI CTAG 2 cut(s) 203, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.