Rh5AG080300

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009088
Physical Location & Seq
Reverse (-)
6863460 .. 6867488
4029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG080300.1

Sequence Viewer

Length: 720 bp
ATGAGGCCGAAGATTTACCTTTTTGGTGATTCGATCACGGAGGAGTCGTTCGGCGACGGTGGCTGGGGTGCTTCTCTTGCCCACCAATTCTCTCGCACGGTTGATGTGGTGCTGAGAGGTTATAGCGGGTACAACACTCGGTGGGCTCAGAAGGTTCTGGAGAGGGTTTTTCCGGGGAGTCAAGACGGCGGCGGTGAAGCGCCGTTAGCTGTCACCGTCTTCTTCGGCGCTAATGACGCTTGCCTTCCCGATAGATGCTCTGGTTTTCAGCACGTGCCGGTTGACGAGTACAAGCAGAACCTCCGCTCCATTGTCTCCTTTCTCAAGAAGCAATGGCCTACTACTCACATTATTCTCATCACACCTCCTCCGATTGATGAAGATGGACGCCTTCAATTTCCCTATATTGAGAATCCATTGAATCAGCCCGAGAGGACAAATGAAGCTGCTGGTGCTTTTGCAGAGGCTTGTGTTGCCGTAGCTCGAGAAAGTGGTCTTCCAGTAATAGATCTCTGGACCAAAATGCAGCAGTTTCCTGACTGGCAGAAAGCTCATCTAAGGGATGGTTTGCACCTTACTCCGCGGGGAAACAGACTCGTTTTTGAGGAAGTGGTTGTGAAGCTGAGGGATGAAGGATTGAGTCTAGAAAGTTTGCCAGTTGATCTCCCACTTATAGCTGATATTGACCCTAATGACCCTCTTAAGGCTTTTCAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

239

Amino Acids

26.69

Weight (kDa)

5.16

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 5 - 204 1e-30 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 7 - 199 1.4e-30 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 306
AccII CGCG 1 cut(s) 583
AciI CCGC 6 cut(s) 126, 189, 192, 304, 581, 583
AcvI CACGTG 1 cut(s) 274
AcyI GRCGYC 1 cut(s) 388
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 2 cut(s) 131, 290
AfiI CCNNNNNNNGG 1 cut(s) 703
AflII CTTAAG 1 cut(s) 701
AgsI TTSAA 2 cut(s) 395, 421
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 290, 322
AluBI AGCT 6 cut(s) 209, 446, 482, 551, 622, 677
AluI AGCT 6 cut(s) 209, 446, 482, 551, 622, 677
Alw26I GTCTC 1 cut(s) 319
Ama87I CYCGRG 2 cut(s) 428, 483
AoxI GGCC 2 cut(s) 5, 335
ApeKI GCWGC 2 cut(s) 446, 526
AspLEI GCGC 2 cut(s) 202, 230
AspS9I GGNCC 1 cut(s) 516
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 3 cut(s) 38, 205, 206
AvaI CYCGRG 2 cut(s) 428, 483
AvaII GGWCC 1 cut(s) 516
BanII GRGCYC 1 cut(s) 148
BbrPI CACGTG 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 211, 488
BbvCI CCTCAGC 1 cut(s) 623
BbvI GCAGC 2 cut(s) 433, 538
BccI CCATC 2 cut(s) 377, 557
BceAI ACGGC 3 cut(s) 187, 202, 461
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 644
BfoI RGCGCY 2 cut(s) 203, 231
BfrI CTTAAG 1 cut(s) 701
BglII AGATCT 1 cut(s) 508
BisI GCNGC 3 cut(s) 190, 447, 527
BlsI GCNGC 3 cut(s) 191, 448, 528
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 516
BmeT110I CYCGRG 2 cut(s) 428, 483
BmgT120I GGNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 245
BpiI GAAGAC 2 cut(s) 211, 488
BpmI CTGGAG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 623
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 388
BsaJI CCNNGG 2 cut(s) 173, 581
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 290, 320, 352, 382
Bsc4I CCNNNNNNNGG 1 cut(s) 703
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 3 cut(s) 500, 545, 656
Bse3DI GCAATG 1 cut(s) 338
BseDI CCNNGG 2 cut(s) 173, 581
BseGI GGATG 2 cut(s) 568, 634
BseLI CCNNNNNNNGG 1 cut(s) 703
BseMI GCAATG 1 cut(s) 338
BseMII CTCAG 3 cut(s) 104, 161, 614
BseNI ACTGG 3 cut(s) 500, 545, 656
BseRI GAGGAG 2 cut(s) 56, 357
BseXI GCAGC 2 cut(s) 433, 538
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 1 cut(s) 583
BshFI GGCC 2 cut(s) 7, 337
BsiHKCI CYCGRG 2 cut(s) 428, 483
BsiSI CCGG 2 cut(s) 173, 278
BslI CCNNNNNNNGG 1 cut(s) 703
BsmAI GTCTC 1 cut(s) 319
BsnI GGCC 2 cut(s) 7, 337
BsoBI CYCGRG 2 cut(s) 428, 483
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 33, 508, 661
BspACI CCGC 6 cut(s) 126, 189, 192, 304, 581, 583
BspANI GGCC 2 cut(s) 7, 337
BspCNI CTCAG 3 cut(s) 105, 160, 615
BspFNI CGCG 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 277
BsrI ACTGG 3 cut(s) 500, 545, 656
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 173, 581
BssMI GATC 3 cut(s) 33, 508, 661
BssNI GRCGYC 1 cut(s) 388
Bst4CI ACNGT 3 cut(s) 59, 100, 217
BstACI GRCGYC 1 cut(s) 388
BstAFI CTTAAG 1 cut(s) 701
BstBAI YACGTR 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 4 cut(s) 113, 147, 557, 623
BstDSI CCRYGG 1 cut(s) 581
BstF5I GGATG 2 cut(s) 568, 634
BstFNI CGCG 1 cut(s) 583
BstH2I RGCGCY 2 cut(s) 203, 231
BstHHI GCGC 2 cut(s) 202, 230
BstKTI GATC 3 cut(s) 36, 511, 664
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 3 cut(s) 33, 508, 661
BstMWI GCNNNNNNNGC 6 cut(s) 60, 77, 206, 236, 452, 473
BstSCI CCNGG 1 cut(s) 172
BstUI CGCG 1 cut(s) 583
BstV1I GCAGC 2 cut(s) 433, 538
BstV2I GAAGAC 2 cut(s) 211, 488
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BsuRI GGCC 2 cut(s) 7, 337
BtgI CCRYGG 1 cut(s) 581
BtsCI GGATG 2 cut(s) 568, 634
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 2 cut(s) 202, 230
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 516
Cfr42I CCGCGG 1 cut(s) 584
CseI GACGC 2 cut(s) 245, 396
Csp6I GTAC 2 cut(s) 130, 289
CviQI GTAC 2 cut(s) 130, 289
DdeI CTNAG 4 cut(s) 113, 147, 557, 623
DpnI GATC 3 cut(s) 35, 510, 663
DpnII GATC 3 cut(s) 33, 508, 661
DraIII CACNNNGTG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 516
Eco72I CACGTG 1 cut(s) 274
Eco88I CYCGRG 2 cut(s) 428, 483
EcoT38I GRGCYC 1 cut(s) 148
FaiI YATR 3 cut(s) 123, 405, 674
FauI CCCGC 2 cut(s) 119, 576
Fnu4HI GCNGC 3 cut(s) 190, 447, 527
FokI GGATG 2 cut(s) 575, 641
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 3 cut(s) 190, 447, 527
FspBI CTAG 1 cut(s) 644
GlaI GCGC 2 cut(s) 201, 229
GluI GCNGC 3 cut(s) 190, 447, 527
GsaI CCCAGC 1 cut(s) 67
GsuI CTGGAG 1 cut(s) 179
HaeII RGCGCY 2 cut(s) 203, 231
HaeIII GGCC 2 cut(s) 7, 337
HapII CCGG 2 cut(s) 173, 278
HgaI GACGC 2 cut(s) 245, 396
HhaI GCGC 2 cut(s) 202, 230
Hin1I GRCGYC 1 cut(s) 388
Hin6I GCGC 2 cut(s) 200, 228
HinP1I GCGC 2 cut(s) 200, 228
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 7 cut(s) 29, 44, 178, 412, 421, 594, 640
HpaII CCGG 2 cut(s) 173, 278
HphI GGTGA 3 cut(s) 38, 205, 206
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 3 cut(s) 150, 372, 714
Hpy188III TCNNGA 8 cut(s) 158, 182, 248, 325, 485, 514, 536, 644
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 4 cut(s) 145, 254, 401, 626
HpyCH4III ACNGT 3 cut(s) 59, 100, 217
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 3 cut(s) 461, 526, 571
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 77, 206, 236, 452, 473
HpyF3I CTNAG 4 cut(s) 113, 147, 557, 623
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 388
HspAI GCGC 2 cut(s) 200, 228
KspI CCGCGG 1 cut(s) 584
Kzo9I GATC 3 cut(s) 33, 508, 661
LmnI GCTCC 1 cut(s) 311
Lsp1109I GCAGC 2 cut(s) 433, 538
LweI GCATC 1 cut(s) 245
MaeI CTAG 1 cut(s) 644
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 211
MalI GATC 3 cut(s) 35, 510, 663
MbiI CCGCTC 1 cut(s) 306
MboI GATC 3 cut(s) 33, 508, 661
MboII GAAGA 5 cut(s) 22, 211, 214, 392, 488
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 2 cut(s) 86, 395
MlyI GAGTC 4 cut(s) 53, 187, 588, 649
MseI TTAA 1 cut(s) 702
MspA1I CMGCKG 1 cut(s) 583
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 2 cut(s) 173, 278
MspR9I CCNGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 583
MwoI GCNNNNNNNGC 6 cut(s) 60, 77, 206, 236, 452, 473
NciI CCSGG 1 cut(s) 174
NdeII GATC 3 cut(s) 33, 508, 661
NmuCI GTSAC 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 3 cut(s) 29, 412, 421
PkrI GCNGC 3 cut(s) 191, 448, 528
PleI GAGTC 4 cut(s) 52, 186, 588, 648
PmaCI CACGTG 1 cut(s) 274
PmlI CACGTG 1 cut(s) 274
PpsI GAGTC 4 cut(s) 52, 186, 588, 648
Ppu21I YACGTR 1 cut(s) 274
PspCI CACGTG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 516
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 2 cut(s) 131, 290
RsaNI GTAC 2 cut(s) 130, 289
SacII CCGCGG 1 cut(s) 584
SaqAI TTAA 1 cut(s) 702
SatI GCNGC 3 cut(s) 190, 447, 527
Sau3AI GATC 3 cut(s) 33, 508, 661
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 4 cut(s) 53, 187, 588, 649
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 1 cut(s) 245
Sfr274I CTCGAG 1 cut(s) 483
Sfr303I CCGCGG 1 cut(s) 584
SgrBI CCGCGG 1 cut(s) 584
SinI GGWCC 1 cut(s) 516
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 3 cut(s) 323, 483, 701
SmoI CTYRAG 3 cut(s) 323, 483, 701
Sse9I AATT 2 cut(s) 86, 395
SsiI CCGC 6 cut(s) 126, 189, 192, 304, 581, 583
SspMI CTAG 1 cut(s) 644
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 59, 100, 217
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 32, 484
TasI AATT 2 cut(s) 86, 395
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 3 cut(s) 29, 412, 421
Tru1I TTAA 1 cut(s) 702
Tru9I TTAA 1 cut(s) 702
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 446, 526
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 3 cut(s) 393, 456, 645
TspGWI ACGGA 1 cut(s) 53
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 516
XbaI TCTAGA 1 cut(s) 643
XhoI CTCGAG 1 cut(s) 483
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.