Rorug04G0449400

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009088
Physical Location & Seq
Forward (+)
60446088 .. 60449953
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0449400.1

Sequence Viewer

Length: 1908 bp
ATGAAATCTGTTAGAAGTGAAATGTTGTCCAGTCGAAAGATTTTAAATGCTCATCTCTCTTCAAGTCCAAAAATATATTTGTGGGATGACAAACTGATTCGTGATTTTCGTGATCTGAAAAATTATTTGAAAGTCAAACAATCTGAAAAGCAGTTTTCTGTAGATTCACTGGTGTACTTCTGGAATGTTTGGAAGGATATGGTAGTTTACTTGATTGAATATTTTGAATCTGTTGAAACCCAAGCTGTTAATAGAAAGAGAACTTTCGGAGACTTCTTTCTGAATTACCTGGGAGTGTGGAGGCAGTTTCATGATGATCTGCGTTTTGTCTACACTGTACTTATCTCTGATGCAAATTGGGTAAGTGGAATGGACAAAAGGTCTTTTCAAATTAACGGGGAGCTGGTCACCATAGATGTTTGGCAGATAATTTCAGCCGCTCATAGCTATTGGACTTCAGAACTTCTTTCATTTGGCATTAGGATTTTGAATAAGCTTGAAGCGCTTTACAGGGTCAAATCTGATTCAGTTCTCTGCCAAAGCTGGTGTCTTACGCAGATTTATGTTGTTGCGAAGTGTCTCTTGGAATCCAAATATCTGAAGCTAAGGAACGAAGATTCCTATACACTACAGAATTTTGTTGAACTGTCAACTGAAGATATTGTTGCTTGTATATTTCCTCATGATTGGAGGAAATCTGTAAGAGAGAACATGATTACTCTTAGACAAAAGGGTACTTCTAAAAGTTTGCTCAAACAAGTTGTAGTTGATTTACTTACCAGCTCAAAGAAAAATCTTTCTTATGGCCAAGTTGGAAGGCTGGCAATGATTATTCTTGGGTCTGGTAATTTTGATGATGATGAACTATACAAGAAACTTATGAAAAATCTGAAATGCAGTCCACCGTGGAAGGTGTTCATTCATAATCTGTGTGGGAGTATCATTGCAAAACAGAAAGGGTCTGTACTTATCAATACCAGTCAAGAAAAGAAAGATGTGTCTCTTGTCCAGGGCTTTCATCAGGCTTTGGCAGAAACTTATTATGCAAACTGGAGGGAGCTAGGTTTTGTTATCACCACCAAATCATGTTTCATTGAATGGTTGATGCAGAAGGAAGATACCCAACTCACTTCCAGTATACTGGCTGATGTGCTAATATCTTTTGAACCCATCCTTATGTTTCTGATCGCTGTGGTTCGGGAGGTTCTTTATGATAAGAAGACCATGGTTGAATGGATTCAAAGGTCTATCTCACAGTGGGAGGAGTGTCATTCGCTGCTGATCCTGAGATCGGTTGTTATCTTGTGTTTGCTTTATTTGAATTTTGGAATGGGGTATGATATACTCCTAGATCTACTGTGTAGGGAAGATATCACTGAACGACTCCCAAAGGATTTTTGCAATGCCTTGAGGAGAATGCATAAATGTTCTCTTAATGAAAATGTGAATGCTCTCGCTAAAGCTTTTAAGAAGATTGGTAATGATTTGGTGGTTACAAGTTTTGGGGTTGATTGTTCAAGGTTCTTTTGTCCAGATTCCATTTCTGTAGAGATGAAGGCCAACCTATGCAGTAATGAGATACTCAGAAAATTGTTTCAAAGACCACATATTCATGTTCAATATTCCCAAGGTCAAACTATTATCGGGGAAGTTTGCAGTCCTGTTCCTTCTGCTTATGGTTCGGAGGAGACCAAGGTTATTGAGAGTCCTAGGCTTCTTCTATCCAACTCTGGTGTGGTGGAAGATGAGGTCCAACACACTGGAAACAGTAACAAAAGTGACCTTCCAATGGGTTTTGATGCTTTGTGGGACAAATTTAAAAGTTTTAAGTTGGTGGAGAAGGATGGTCAGAGGAGTTTACTTTCAGATGCCTCAGCATTTAAGGAGAAATGGATTAGGCGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

635

Amino Acids

73.47

Weight (kDa)

8.5

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 440
AccI GTMKAC 2 cut(s) 330, 1138
AciI CCGC 1 cut(s) 438
AclWI GGATC 1 cut(s) 1276
AcoI YGGCCR 1 cut(s) 805
AcsI RAATTY 3 cut(s) 634, 1321, 1814
AcuI CTGAAG 3 cut(s) 441, 620, 675
AfaI GTAC 4 cut(s) 176, 339, 736, 966
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 2 cut(s) 1291, 1789
AhdI GACNNNNNGTC 1 cut(s) 379
AjnI CCWGG 2 cut(s) 288, 1008
AjuI GAANNNNNNNTTGG 4 cut(s) 566, 598, 1073, 1105
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 9 cut(s) 245, 403, 447, 496, 543, 604, 783, 1060, 1463
AluI AGCT 9 cut(s) 245, 403, 447, 496, 543, 604, 783, 1060, 1463
Alw26I GTCTC 4 cut(s) 264, 584, 1005, 1682
AlwI GGATC 1 cut(s) 1276
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 2 cut(s) 805, 1557
ApeKI GCWGC 1 cut(s) 1276
ApoI RAATTY 3 cut(s) 634, 1321, 1814
ArsI GACNNNNNNTTYG 2 cut(s) 532, 564
Asp700I GAANNNNTTC 2 cut(s) 914, 1236
AspA2I CCTAGG 1 cut(s) 1709
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 1750
AsuHPI GGTGA 2 cut(s) 400, 1066
AvaII GGWCC 1 cut(s) 1750
AvrII CCTAGG 1 cut(s) 1709
BalI TGGCCA 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 1226
BbvCI CCTCAGC 1 cut(s) 1873
BbvI GCAGC 1 cut(s) 1263
BccI CCATC 2 cut(s) 1178, 1838
BciT130I CCWGG 2 cut(s) 290, 1010
BcoDI GTCTC 4 cut(s) 264, 584, 1005, 1682
BfaI CTAG 3 cut(s) 1061, 1349, 1710
BfmI CTRYAG 3 cut(s) 159, 629, 1545
BfoI RGCGCY 1 cut(s) 506
BglII AGATCT 1 cut(s) 1351
BisI GCNGC 2 cut(s) 438, 1277
BlnI CCTAGG 1 cut(s) 1709
BlsI GCNGC 2 cut(s) 439, 1278
Bme1390I CCNGG 2 cut(s) 290, 1010
Bme18I GGWCC 1 cut(s) 1750
BmeRI GACNNNNNGTC 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 1750
BmrFI CCNGG 2 cut(s) 290, 1010
BmsI GCATC 4 cut(s) 340, 1095, 1789, 1858
BpiI GAAGAC 1 cut(s) 1226
BpmI CTGGAG 1 cut(s) 1072
Bpu10I CCTNAGC 2 cut(s) 605, 1873
BpuEI CTTGAG 1 cut(s) 1429
BsaI GGTCTC 1 cut(s) 1682
BsaJI CCNNGG 7 cut(s) 289, 905, 1009, 1224, 1627, 1692, 1709
Bsc4I CCNNNNNNNGG 2 cut(s) 1291, 1789
Bse1I ACTGG 7 cut(s) 30, 174, 978, 1055, 1134, 1146, 1765
Bse3DI GCAATG 3 cut(s) 831, 942, 1408
BseBI CCWGG 2 cut(s) 290, 1010
BseDI CCNNGG 7 cut(s) 289, 905, 1009, 1224, 1627, 1692, 1709
BseGI GGATG 3 cut(s) 91, 1170, 1849
BseLI CCNNNNNNNGG 2 cut(s) 1291, 1789
BseMI GCAATG 3 cut(s) 831, 942, 1408
BseMII CTCAG 3 cut(s) 1277, 1597, 1887
BseNI ACTGG 7 cut(s) 30, 174, 978, 1055, 1134, 1146, 1765
BseRI GAGGAG 4 cut(s) 1277, 1426, 1700, 1867
BseXI GCAGC 1 cut(s) 1263
BshFI GGCC 2 cut(s) 807, 1559
BslFI GGGAC 1 cut(s) 1823
BslI CCNNNNNNNGG 2 cut(s) 1291, 1789
BsmAI GTCTC 4 cut(s) 264, 584, 1005, 1682
BsmFI GGGAC 1 cut(s) 1823
BsmI GAATGC 2 cut(s) 1422, 1453
BsnI GGCC 2 cut(s) 807, 1559
Bso31I GGTCTC 1 cut(s) 1682
Bsp143I GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
Bsp19I CCATGG 1 cut(s) 1224
BspACI CCGC 1 cut(s) 438
BspANI GGCC 2 cut(s) 807, 1559
BspCNI CTCAG 3 cut(s) 1278, 1596, 1886
BspHI TCATGA 2 cut(s) 310, 682
BspPI GGATC 1 cut(s) 1276
BspTNI GGTCTC 1 cut(s) 1682
BsrBI CCGCTC 1 cut(s) 440
BsrDI GCAATG 3 cut(s) 831, 942, 1408
BsrI ACTGG 7 cut(s) 30, 174, 978, 1055, 1134, 1146, 1765
BssECI CCNNGG 7 cut(s) 289, 905, 1009, 1224, 1627, 1692, 1709
BssMI GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
BssNAI GTATAC 1 cut(s) 1139
BssT1I CCWWGG 4 cut(s) 1224, 1627, 1692, 1709
Bst1107I GTATAC 1 cut(s) 1139
Bst2UI CCWGG 2 cut(s) 290, 1010
Bst4CI ACNGT 6 cut(s) 337, 648, 906, 1257, 1359, 1769
Bst6I CTCTTC 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 5 cut(s) 605, 722, 1286, 1583, 1873
BstDSI CCRYGG 2 cut(s) 905, 1224
BstEII GGTNACC 1 cut(s) 406
BstF5I GGATG 3 cut(s) 91, 1170, 1849
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 6 cut(s) 115, 319, 1188, 1284, 1292, 1354
BstMAI GTCTC 4 cut(s) 264, 584, 1005, 1682
BstMBI GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNI CCWGG 2 cut(s) 290, 1010
BstPI GGTNACC 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 288, 1008
BstSFI CTRYAG 3 cut(s) 159, 629, 1545
BstV1I GCAGC 1 cut(s) 1263
BstV2I GAAGAC 1 cut(s) 1226
BstX2I RGATCY 1 cut(s) 1351
BstXI CCANNNNNNTGG 2 cut(s) 1141, 1760
BstYI RGATCY 1 cut(s) 1351
BstZ17I GTATAC 1 cut(s) 1139
BsuRI GGCC 2 cut(s) 807, 1559
BtgI CCRYGG 2 cut(s) 905, 1224
BtsCI GGATG 3 cut(s) 91, 1170, 1849
BtsIMutI CAGTG 5 cut(s) 167, 333, 1262, 1374, 1758
Cac8I GCNNGC 1 cut(s) 822
CciI TCATGA 2 cut(s) 310, 682
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 1750
Csp6I GTAC 4 cut(s) 175, 338, 735, 965
CviAII CATG 6 cut(s) 311, 683, 712, 1086, 1225, 1613
CviQI GTAC 4 cut(s) 175, 338, 735, 965
DdeI CTNAG 5 cut(s) 605, 722, 1286, 1583, 1873
DpnI GATC 6 cut(s) 114, 318, 1187, 1283, 1291, 1353
DpnII GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
DraI TTTAAA 2 cut(s) 45, 1819
DriI GACNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 805
Eam1104I CTCTTC 1 cut(s) 64
Eam1105I GACNNNNNGTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 64
Eco130I CCWWGG 4 cut(s) 1224, 1627, 1692, 1709
Eco31I GGTCTC 1 cut(s) 1682
Eco32I GATATC 1 cut(s) 1372
Eco47I GGWCC 1 cut(s) 1750
Eco47III AGCGCT 1 cut(s) 504
Eco57I CTGAAG 3 cut(s) 441, 620, 675
Eco91I GGTNACC 1 cut(s) 406
EcoO65I GGTNACC 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 288, 1008
EcoRV GATATC 1 cut(s) 1372
EcoT14I CCWWGG 4 cut(s) 1224, 1627, 1692, 1709
EcoT22I ATGCAT 1 cut(s) 1422
ErhI CCWWGG 4 cut(s) 1224, 1627, 1692, 1709
FaeI CATG 6 cut(s) 314, 686, 715, 1089, 1228, 1616
FalI AAGNNNNNCTT 2 cut(s) 566, 598
FaqI GGGAC 1 cut(s) 1823
FatI CATG 6 cut(s) 310, 682, 711, 1085, 1224, 1612
FblI GTMKAC 2 cut(s) 330, 1138
Fnu4HI GCNGC 2 cut(s) 438, 1277
FokI GGATG 3 cut(s) 98, 1157, 1856
Fsp4HI GCNGC 2 cut(s) 438, 1277
FspBI CTAG 3 cut(s) 1061, 1349, 1710
GlaI GCGC 1 cut(s) 504
GluI GCNGC 2 cut(s) 438, 1277
GsuI CTGGAG 1 cut(s) 1072
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 2 cut(s) 807, 1559
HhaI GCGC 1 cut(s) 505
Hin1II CATG 6 cut(s) 314, 686, 715, 1089, 1228, 1616
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 2 cut(s) 494, 1461
HphI GGTGA 2 cut(s) 400, 1066
Hpy166II GTNNAC 7 cut(s) 175, 208, 331, 651, 902, 1139, 1859
Hpy188III TCNNGA 9 cut(s) 101, 110, 181, 311, 683, 983, 1199, 1285, 1532
Hpy8I GTNNAC 7 cut(s) 175, 208, 331, 651, 902, 1139, 1859
HpyAV CCTTC 8 cut(s) 187, 810, 904, 1105, 1549, 1677, 1793, 1834
HpyCH4III ACNGT 6 cut(s) 337, 648, 906, 1257, 1359, 1769
HpyCH4V TGCA 9 cut(s) 353, 897, 947, 1046, 1108, 1401, 1420, 1569, 1656
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 5 cut(s) 605, 722, 1286, 1583, 1873
Hsp92II CATG 6 cut(s) 314, 686, 715, 1089, 1228, 1616
HspAI GCGC 1 cut(s) 503
Kzo9I GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
LmnI GCTCC 2 cut(s) 400, 1057
Lsp1109I GCAGC 1 cut(s) 1263
LweI GCATC 4 cut(s) 340, 1095, 1789, 1858
MaeI CTAG 3 cut(s) 1061, 1349, 1710
MaeIII GTNAC 4 cut(s) 406, 1492, 1769, 1778
MalI GATC 6 cut(s) 114, 318, 1187, 1283, 1291, 1353
MbiI CCGCTC 1 cut(s) 440
MboI GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
MboII GAAGA 9 cut(s) 51, 626, 668, 1127, 1231, 1379, 1483, 1709, 1754
MflI RGATCY 1 cut(s) 1351
MlsI TGGCCA 1 cut(s) 807
MluNI TGGCCA 1 cut(s) 807
MlyI GAGTC 2 cut(s) 1377, 1714
MmeI TCCRAC 3 cut(s) 793, 1749, 1777
Mox20I TGGCCA 1 cut(s) 807
Mph1103I ATGCAT 1 cut(s) 1422
MroXI GAANNNNTTC 2 cut(s) 914, 1236
MscI TGGCCA 1 cut(s) 807
MseI TTAA 8 cut(s) 44, 249, 393, 1434, 1467, 1818, 1827, 1881
MslI CAYNNNNRTG 2 cut(s) 1175, 1611
Msp20I TGGCCA 1 cut(s) 807
MspR9I CCNGG 2 cut(s) 290, 1010
Mva1269I GAATGC 2 cut(s) 1422, 1453
MvaI CCWGG 2 cut(s) 290, 1010
MwoI GCNNNNNNNGC 1 cut(s) 502
NcoI CCATGG 1 cut(s) 1224
NdeII GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
NlaIII CATG 6 cut(s) 314, 686, 715, 1089, 1228, 1616
NmuCI GTSAC 2 cut(s) 406, 1778
NsiI ATGCAT 1 cut(s) 1422
PagI TCATGA 2 cut(s) 310, 682
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PctI GAATGC 2 cut(s) 1422, 1453
PdmI GAANNNNTTC 2 cut(s) 914, 1236
PfeI GAWTC 8 cut(s) 97, 164, 227, 524, 587, 617, 1237, 1535
PkrI GCNGC 2 cut(s) 439, 1278
PleI GAGTC 2 cut(s) 1377, 1713
PpsI GAGTC 2 cut(s) 1377, 1713
Psp6I CCWGG 2 cut(s) 288, 1008
PspEI GGTNACC 1 cut(s) 406
PspGI CCWGG 2 cut(s) 288, 1008
PspPI GGNCC 1 cut(s) 1750
PsuI RGATCY 1 cut(s) 1351
RsaI GTAC 4 cut(s) 176, 339, 736, 966
RsaNI GTAC 4 cut(s) 175, 338, 735, 965
RseI CAYNNNNRTG 2 cut(s) 1175, 1611
SaqAI TTAA 8 cut(s) 44, 249, 393, 1434, 1467, 1818, 1827, 1881
SatI GCNGC 2 cut(s) 438, 1277
Sau3AI GATC 6 cut(s) 112, 316, 1185, 1281, 1289, 1351
Sau96I GGNCC 1 cut(s) 1750
SchI GAGTC 2 cut(s) 1377, 1714
ScrFI CCNGG 2 cut(s) 290, 1010
SfaNI GCATC 4 cut(s) 340, 1095, 1789, 1858
SfcI CTRYAG 3 cut(s) 159, 629, 1545
SinI GGWCC 1 cut(s) 1750
SmiMI CAYNNNNRTG 2 cut(s) 1175, 1611
SmlI CTYRAG 1 cut(s) 1408
SmoI CTYRAG 1 cut(s) 1408
SsiI CCGC 1 cut(s) 438
SspI AATATT 2 cut(s) 221, 1622
SspMI CTAG 3 cut(s) 1061, 1349, 1710
StyD4I CCNGG 2 cut(s) 288, 1008
StyI CCWWGG 4 cut(s) 1224, 1627, 1692, 1709
TaaI ACNGT 6 cut(s) 337, 648, 906, 1257, 1359, 1769
TaqI TCGA 1 cut(s) 34
TatI WGTACW 3 cut(s) 174, 337, 964
TauI GCSGC 1 cut(s) 440
TfiI GAWTC 8 cut(s) 97, 164, 227, 524, 587, 617, 1237, 1535
Tru1I TTAA 8 cut(s) 44, 249, 393, 1434, 1467, 1818, 1827, 1881
Tru9I TTAA 8 cut(s) 44, 249, 393, 1434, 1467, 1818, 1827, 1881
TscAI CASTG 5 cut(s) 174, 340, 1262, 1381, 1765
TseFI GTSAC 2 cut(s) 406, 1778
TseI GCWGC 1 cut(s) 1276
Tsp45I GTSAC 2 cut(s) 406, 1778
TspRI CASTG 5 cut(s) 174, 340, 1262, 1381, 1765
VpaK11BI GGWCC 1 cut(s) 1750
XapI RAATTY 3 cut(s) 634, 1321, 1814
XcmI CCANNNNNNNNNTGG 1 cut(s) 1732
XmaJI CCTAGG 1 cut(s) 1709
XmiI GTMKAC 2 cut(s) 330, 1138
XmnI GAANNNNTTC 2 cut(s) 914, 1236
XspI CTAG 3 cut(s) 1061, 1349, 1710
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.