Prupe.5G006000_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0007456
Physical Location & Seq
Forward (+)
691146 .. 695176
4031 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G006000.1

Sequence Viewer

Length: 417 bp
ATGGGAGTGGAAAATGTCGCTGATCTTCCACCTCTGGACCCAGCTGTTGTTTTGATTGGAACAGCAAAGGGAGGTAATGTTGGACCTCCTAGCGGTCTTGTTGACATTGGTGTGAGTGAAGCTGCCTACCTTTTTCGAGTTGCACTTCCTGGTATTCGGAAGAATGAATGTAGGGTAAAATGTGATATCCAACGTGATGGAAAGGTTCATATAGAAGGAATCATGACAGGTGTCGGACTTTTGAGAAACTCGTCAACTGTGTACCATTCGAGAGTCCAGCAACTATGCCCACCGGGAGAATTTAGCATATCTTTCCGTCTGCCTGGACCTGTTGACCCCCGTTTGTTTTCTCCTTATTTCCGGCATGATGGAATCCTGGAAGTGGTGGTTATGAAAGGCAGAGCACCTCTACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

139

Amino Acids

14.81

Weight (kDa)

8.54

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 93
AcsI RAATTY 1 cut(s) 299
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 92, 382
AjnI CCWGG 3 cut(s) 148, 322, 375
AluBI AGCT 2 cut(s) 44, 122
AluI AGCT 2 cut(s) 44, 122
Alw21I GWGCWC 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 1 cut(s) 299
AspS9I GGNCC 3 cut(s) 37, 83, 326
AsuC2I CCSGG 1 cut(s) 294
AvaII GGWCC 3 cut(s) 37, 83, 326
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 109
BccI CCATC 2 cut(s) 191, 362
BciT130I CCWGG 3 cut(s) 150, 324, 377
BcnI CCSGG 1 cut(s) 294
BfaI CTAG 1 cut(s) 90
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 4 cut(s) 150, 294, 324, 377
Bme18I GGWCC 3 cut(s) 37, 83, 326
BmgT120I GGNCC 3 cut(s) 37, 83, 326
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 4 cut(s) 150, 294, 324, 377
BoxI GACNNNNGTC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 382
BseBI CCWGG 3 cut(s) 150, 324, 377
BseLI CCNNNNNNNGG 2 cut(s) 92, 382
BseXI GCAGC 1 cut(s) 109
BseYI CCCAGC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 2 cut(s) 293, 361
BslI CCNNNNNNNGG 2 cut(s) 92, 382
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 1 cut(s) 93
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 39
BssMI GATC 1 cut(s) 22
Bst2UI CCWGG 3 cut(s) 150, 324, 377
Bst4CI ACNGT 1 cut(s) 259
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstNI CCWGG 3 cut(s) 150, 324, 377
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 148, 292, 322, 375
BstV1I GCAGC 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 197
CciI TCATGA 1 cut(s) 222
Cfr13I GGNCC 3 cut(s) 37, 83, 326
Csp6I GTAC 1 cut(s) 262
CviAII CATG 2 cut(s) 223, 365
CviJI RGCY 2 cut(s) 44, 122
CviKI_1 RGCY 2 cut(s) 44, 122
CviQI GTAC 1 cut(s) 262
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 3 cut(s) 37, 83, 326
EcoRII CCWGG 3 cut(s) 148, 322, 375
EcoRV GATATC 1 cut(s) 187
FaeI CATG 2 cut(s) 226, 368
FaiI YATR 7 cut(s) 210, 212, 224, 286, 308, 366, 392
FatI CATG 2 cut(s) 222, 364
Fnu4HI GCNGC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 90
GluI GCNGC 1 cut(s) 123
GsaI CCCAGC 1 cut(s) 44
HapII CCGG 2 cut(s) 293, 361
Hin1II CATG 2 cut(s) 226, 368
HincII GTYRAC 3 cut(s) 103, 255, 334
HindII GTYRAC 3 cut(s) 103, 255, 334
HinfI GANTC 3 cut(s) 219, 273, 372
HpaII CCGG 2 cut(s) 293, 361
Hpy166II GTNNAC 4 cut(s) 103, 255, 262, 334
Hpy188I TCNGA 2 cut(s) 159, 236
Hpy188III TCNNGA 3 cut(s) 35, 223, 270
Hpy8I GTNNAC 4 cut(s) 103, 255, 262, 334
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 193
HpyCH4V TGCA 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 193
Hsp92II CATG 2 cut(s) 226, 368
Kzo9I GATC 1 cut(s) 22
Lsp1109I GCAGC 1 cut(s) 109
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 193
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 17, 172
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 1 cut(s) 299
MlyI GAGTC 1 cut(s) 282
MmeI TCCRAC 3 cut(s) 61, 214, 214
MnlI CCTC 4 cut(s) 42, 65, 96, 417
MslI CAYNNNNRTG 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 2 cut(s) 293, 361
MspR9I CCNGG 4 cut(s) 150, 294, 324, 377
MvaI CCWGG 3 cut(s) 150, 324, 377
NciI CCSGG 1 cut(s) 294
NdeII GATC 1 cut(s) 22
NlaIII CATG 2 cut(s) 226, 368
NlaIV GGNNCC 1 cut(s) 39
PagI TCATGA 1 cut(s) 222
PfeI GAWTC 2 cut(s) 219, 372
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 124
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 230
Psp6I CCWGG 3 cut(s) 148, 322, 375
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 3 cut(s) 148, 322, 375
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 37, 83, 326
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 110
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 3 cut(s) 37, 83, 326
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 4 cut(s) 150, 294, 324, 377
SduI GDGCHC 1 cut(s) 406
SinI GGWCC 3 cut(s) 37, 83, 326
SmiMI CAYNNNNRTG 1 cut(s) 110
Sse9I AATT 1 cut(s) 299
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 4 cut(s) 148, 292, 322, 375
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 136, 269
TasI AATT 1 cut(s) 299
TfiI GAWTC 2 cut(s) 219, 372
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 3 cut(s) 180, 197, 407
TspGWI ACGGA 1 cut(s) 305
VpaK11BI GGWCC 3 cut(s) 37, 83, 326
XapI RAATTY 1 cut(s) 299
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.