Rorug04G0096500

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002786
Physical Location & Seq
Reverse (-)
15171763 .. 15179346
7584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0096500.1

Sequence Viewer

Length: 1608 bp
ATGGGAAAGAAATCTGCATCGTCTTTATCCTCACCGTCGTCGCCGCCTTCGTGGTGGTGGTGGAGATATCGATCGGCACTCGTCATAATCTCATTCGTGGCTCTCACATGGCTGCTCTTCGACCACCAATCCCTCACCTTGGAGGACCAGGTAGTGGTGTCCAGGTTGAATAAGGAGCTGAAAATATTGAAGGATCAGTTAGAAAATGTGAAGAAGCATATAAGAGGCTGGAGCATTGTTGAAGAGGATATGGATGATCCTCTCAATGTAGAACGAAGAAATGCGGTTAAGGATGCTATGGTTCATGCCTGGACTTGTTACGAGAAGTATGCGTGGGGTCGTGATGAACTCCAGCCACAGACAAAAGACGCTGCTGACACTTTCGGTGGTCTAGGAGCAACTCTAGTGGATTCTCTTGATACATTGTATATAATGGGTCTTGACCAGCAATTCCAAAGAGCTAGAGAGTGGGTTGCAAGCTCATTGAATTTCAACAAGAATTACGAGGCTAGTGTTTTTGAGACAACCATAAGAGTTATAGGCGGACTTCTTAGCGCATATGATCTGTCAGATGACAAACTCTTCCTTGACAAGGCTAGAGATATTGCAGATAGGTTGCTGCCTGCATGGAATACACCTTCTGGAATCCCATACAACATGATCAACTTGATGTATGGTAATGCCCATAACCCTAGGTGGGCAGGGGGAAAAAGTATTCTTGCAGATGCTGCTTCAGAACAGCTTGAATTTATTGCTTTATCTCAACGGACGGAAGACCCCAAGTACCAGCAGAAGGTGGAATATGTCATCAAAGAGTTTCATAAAATCTTCCCTGCTGATGGATTGCTTCCCATATATCTTGATCCTCACACTGGAACAATGTCATACTCAAAAGTTACATTCGGTGCCTTGGGTGATAGTTTTTATGAATATCTACTCAAGGCTTGGATACAAGGAAACAAAACAGAAGCTGTAAAACATTACAGAGAAATGTGGGAGACATCAATGAAAGGTTTGAAAAGCTTGATTCGGAGGACAACACCATCCTCGTTTGCATATATATGTGAAAAGCTTGGAAGCTCACTAACTGACAAGATGGATGAATTAGCTTGCTTTGCTCCTGGCATGCTGGCTTTAGGATCTTCTGGCTATGACCCTGGCGAAGCTGAAAAGATTTTATCCCTTGCTGAAGAGCTTGCATGGACATGTTACAATTTCTATCAGACAACGCCTACAAAATTGGCTGGGGAGAACTATAACTTTCCCGCTGGACAGGACATGACCGTGGGAACATCATGGAACATTTTAAGGCCTGAAACTGTTGAGTCACTTTTCTACCTATGGCGGGTAACTGGGAACAAAACTTACCAAGAATGGGGTTGGAACATTTTCCAAGCATTTGAAAATAACTCTCGTACAGATACAGGATATAGTGGACTTAAAGATGTAAGTAAAGGTGATAAAGACAATATGATGCAGAGCTTCTTCCTTGCAGAAACACTGAAGTATCTCTACCTCCTCTTTTCACCTCCATCATTCATCTCTTTGGACGAATGGGTGTTTAACACAGAAGCCCACCCACTAAGGATTGTGACTCAAACCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

535

Amino Acids

61.06

Weight (kDa)

5.27

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_47 PF01532 99 - 530 3.4e-161 Glycosyl hydrolase family 47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AccB7I CCANNNNNTGG 1 cut(s) 154
AciI CCGC 5 cut(s) 44, 284, 543, 1266, 1345
AclWI GGATC 4 cut(s) 201, 251, 857, 1147
AcsI RAATTY 2 cut(s) 487, 746
AcuI CTGAAG 3 cut(s) 717, 1209, 1523
AfaI GTAC 2 cut(s) 785, 1417
AfiI CCNNNNNNNGG 9 cut(s) 139, 154, 592, 697, 793, 839, 872, 1345, 1375
AflIII ACRYGT 1 cut(s) 1205
AgsI TTSAA 8 cut(s) 169, 190, 242, 487, 493, 746, 1018, 1403
AjnI CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
Alw26I GTCTC 2 cut(s) 515, 992
AlwI GGATC 4 cut(s) 201, 251, 857, 1147
AlwNI CAGNNNCTG 2 cut(s) 728, 971
AoxI GGCC 1 cut(s) 1310
ApeKI GCWGC 4 cut(s) 112, 371, 619, 728
ApoI RAATTY 2 cut(s) 487, 746
Asp700I GAANNNNTTC 1 cut(s) 1388
AspA2I CCTAGG 1 cut(s) 692
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 5 cut(s) 24, 127, 926, 1469, 1518
AvaII GGWCC 1 cut(s) 145
AvrII CCTAGG 1 cut(s) 692
BaeI ACNNNNGTAYC 2 cut(s) 1490, 1523
BanI GGYRCC 1 cut(s) 905
BarI GAAGNNNNNNTAC 2 cut(s) 1496, 1528
BbsI GAAGAC 1 cut(s) 780
BbvI GCAGC 4 cut(s) 99, 358, 606, 715
BccI CCATC 4 cut(s) 833, 1051, 1090, 1540
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BciVI GTATCC 1 cut(s) 942
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 515, 992
BfaI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
BfuI GTATCC 1 cut(s) 942
BisI GCNGC 5 cut(s) 44, 113, 372, 620, 729
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 5 cut(s) 45, 114, 373, 621, 730
Bme1390I CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
BmrI ACTGGG 2 cut(s) 1362, 1597
BmsI GCATC 4 cut(s) 26, 283, 715, 1464
BmuI ACTGGG 2 cut(s) 1362, 1597
BpiI GAAGAC 1 cut(s) 780
BpmI CTGGAG 2 cut(s) 250, 335
BpuEI CTTGAG 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 70
BsaJI CCNNGG 5 cut(s) 138, 692, 909, 1156, 1284
Bsc4I CCNNNNNNNGG 9 cut(s) 139, 154, 592, 697, 793, 839, 872, 1345, 1375
Bse1I ACTGG 3 cut(s) 877, 1357, 1603
Bse8I GATNNNNATC 1 cut(s) 70
BseBI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 5 cut(s) 138, 692, 909, 1156, 1284
BseGI GGATG 4 cut(s) 259, 298, 1043, 1105
BseJI GATNNNNATC 1 cut(s) 70
BseLI CCNNNNNNNGG 9 cut(s) 139, 154, 592, 697, 793, 839, 872, 1345, 1375
BseNI ACTGG 3 cut(s) 877, 1357, 1603
BseRI GAGGAG 1 cut(s) 1508
BseXI GCAGC 4 cut(s) 99, 358, 606, 715
BseYI CCCAGC 1 cut(s) 1244
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 1312
BshNI GGYRCC 1 cut(s) 905
BshVI ATCGAT 1 cut(s) 70
BsiEI CGRYCG 1 cut(s) 74
BslI CCNNNNNNNGG 9 cut(s) 139, 154, 592, 697, 793, 839, 872, 1345, 1375
BsmAI GTCTC 2 cut(s) 515, 992
BsnI GGCC 1 cut(s) 1312
Bsp143I GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BspACI CCGC 5 cut(s) 44, 284, 543, 1266, 1345
BspANI GGCC 1 cut(s) 1312
BspDI ATCGAT 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 907
BspPI GGATC 4 cut(s) 201, 251, 857, 1147
BspQI GCTCTTC 2 cut(s) 122, 1185
BspT107I GGYRCC 1 cut(s) 905
BsrI ACTGG 3 cut(s) 877, 1357, 1603
BssECI CCNNGG 5 cut(s) 138, 692, 909, 1156, 1284
BssMI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BssT1I CCWWGG 3 cut(s) 138, 692, 909
Bst2UI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
Bst4CI ACNGT 3 cut(s) 36, 1285, 1321
Bst6I CTCTTC 4 cut(s) 122, 237, 587, 1185
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 6 cut(s) 478, 624, 1111, 1127, 1131, 1197
BstDEI CTNAG 2 cut(s) 551, 1583
BstDSI CCRYGG 1 cut(s) 1284
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 4 cut(s) 259, 298, 1043, 1105
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 7 cut(s) 74, 196, 259, 565, 663, 865, 1142
BstMAI GTCTC 2 cut(s) 515, 992
BstMBI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 2 cut(s) 728, 1115
BstNI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
BstNSI RCATGY 2 cut(s) 1129, 1209
BstSCI CCNGG 5 cut(s) 147, 161, 308, 1120, 1156
BstV1I GCAGC 4 cut(s) 99, 358, 606, 715
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 1 cut(s) 1139
BstYI RGATCY 1 cut(s) 1139
Bsu15I ATCGAT 1 cut(s) 70
BsuI GTATCC 1 cut(s) 942
BsuRI GGCC 1 cut(s) 1312
BsuTUI ATCGAT 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 1284
BtsCI GGATG 4 cut(s) 259, 298, 1043, 1105
BtsIMutI CAGTG 2 cut(s) 870, 1499
Cac8I GCNNGC 6 cut(s) 478, 624, 1111, 1127, 1131, 1197
CaiI CAGNNNCTG 2 cut(s) 728, 971
CfoI GCGC 1 cut(s) 557
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 70
CseI GACGC 1 cut(s) 377
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 2 cut(s) 784, 1416
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 9 cut(s) 108, 305, 627, 658, 1126, 1200, 1206, 1279, 1296
CviQI GTAC 2 cut(s) 784, 1416
DdeI CTNAG 2 cut(s) 551, 1583
DpnI GATC 7 cut(s) 73, 195, 258, 564, 662, 864, 1141
DpnII GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
Eam1104I CTCTTC 4 cut(s) 122, 237, 587, 1185
EarI CTCTTC 4 cut(s) 122, 237, 587, 1185
EciI GGCGGA 1 cut(s) 558
Eco130I CCWWGG 3 cut(s) 138, 692, 909
Eco147I AGGCCT 1 cut(s) 1312
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 145
Eco57I CTGAAG 3 cut(s) 717, 1209, 1523
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoRII CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 3 cut(s) 138, 692, 909
ErhI CCWWGG 3 cut(s) 138, 692, 909
FaeI CATG 9 cut(s) 111, 308, 630, 661, 1129, 1203, 1209, 1282, 1299
FatI CATG 9 cut(s) 107, 304, 626, 657, 1125, 1199, 1205, 1278, 1295
FauI CCCGC 2 cut(s) 1273, 1338
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 44, 113, 372, 620, 729
FokI GGATG 4 cut(s) 266, 305, 1030, 1112
Fsp4HI GCNGC 5 cut(s) 44, 113, 372, 620, 729
FspBI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
GlaI GCGC 1 cut(s) 556
GluI GCNGC 5 cut(s) 44, 113, 372, 620, 729
GsaI CCCAGC 1 cut(s) 1248
GsuI CTGGAG 2 cut(s) 250, 335
HaeIII GGCC 1 cut(s) 1312
HgaI GACGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 557
Hin1II CATG 9 cut(s) 111, 308, 630, 661, 1129, 1203, 1209, 1282, 1299
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HindIII AAGCTT 2 cut(s) 1021, 1070
HinfI GANTC 5 cut(s) 410, 645, 1027, 1325, 1594
HphI GGTGA 5 cut(s) 24, 127, 926, 1469, 1518
Hpy166II GTNNAC 1 cut(s) 1436
Hpy188I TCNGA 4 cut(s) 571, 736, 1032, 1224
Hpy188III TCNNGA 5 cut(s) 341, 416, 440, 642, 860
Hpy8I GTNNAC 1 cut(s) 1436
Hpy99I CGWCG 2 cut(s) 40, 43
HpyAV CCTTC 4 cut(s) 57, 184, 648, 787
HpyCH4III ACNGT 3 cut(s) 36, 1285, 1321
HpyCH4V TGCA 9 cut(s) 17, 476, 608, 626, 722, 1055, 1199, 1477, 1493
HpyF10VI GCNNNNNNNGC 2 cut(s) 728, 1115
HpyF3I CTNAG 2 cut(s) 551, 1583
Hsp92II CATG 9 cut(s) 111, 308, 630, 661, 1129, 1203, 1209, 1282, 1299
HspAI GCGC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
LguI GCTCTTC 2 cut(s) 122, 1185
LmnI GCTCC 4 cut(s) 175, 231, 395, 1123
Lsp1109I GCAGC 4 cut(s) 99, 358, 606, 715
LweI GCATC 4 cut(s) 26, 283, 715, 1464
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
MaeIII GTNAC 6 cut(s) 317, 895, 1208, 1326, 1348, 1591
MalI GATC 7 cut(s) 73, 195, 258, 564, 662, 864, 1141
MboI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
MflI RGATCY 1 cut(s) 1139
MluCI AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
MlyI GAGTC 2 cut(s) 1334, 1588
MmeI TCCRAC 1 cut(s) 1361
MroXI GAANNNNTTC 1 cut(s) 1388
MseI TTAA 4 cut(s) 288, 1307, 1440, 1563
MslI CAYNNNNRTG 3 cut(s) 1060, 1204, 1283
MspA1I CMGCKG 1 cut(s) 1268
MspR9I CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
MvaI CCWGG 5 cut(s) 149, 163, 310, 1122, 1158
MwoI GCNNNNNNNGC 2 cut(s) 728, 1115
NdeI CATATG 1 cut(s) 559
NdeII GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
NlaIII CATG 9 cut(s) 111, 308, 630, 661, 1129, 1203, 1209, 1282, 1299
NlaIV GGNNCC 1 cut(s) 907
NmuCI GTSAC 2 cut(s) 1326, 1591
NspI RCATGY 2 cut(s) 1129, 1209
PaeI GCATGC 1 cut(s) 1129
PceI AGGCCT 1 cut(s) 1312
PciI ACATGT 1 cut(s) 1205
PciSI GCTCTTC 2 cut(s) 122, 1185
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 1388
PfeI GAWTC 3 cut(s) 410, 645, 1027
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 45, 114, 373, 621, 730
Ple19I CGATCG 1 cut(s) 74
PleI GAGTC 2 cut(s) 1333, 1588
PpsI GAGTC 2 cut(s) 1333, 1588
PscI ACATGT 1 cut(s) 1205
Psp6I CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
PspFI CCCAGC 1 cut(s) 1244
PspGI CCWGG 5 cut(s) 147, 161, 308, 1120, 1156
PspN4I GGNNCC 1 cut(s) 907
PspPI GGNCC 1 cut(s) 145
PsrI GAACNNNNNNTAC 2 cut(s) 1349, 1381
PstNI CAGNNNCTG 2 cut(s) 728, 971
PsuI RGATCY 1 cut(s) 1139
PvuI CGATCG 1 cut(s) 74
RsaI GTAC 2 cut(s) 785, 1417
RsaNI GTAC 2 cut(s) 784, 1416
RseI CAYNNNNRTG 3 cut(s) 1060, 1204, 1283
SapI GCTCTTC 2 cut(s) 122, 1185
SaqAI TTAA 4 cut(s) 288, 1307, 1440, 1563
SatI GCNGC 5 cut(s) 44, 113, 372, 620, 729
Sau3AI GATC 7 cut(s) 71, 193, 256, 562, 660, 862, 1139
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 2 cut(s) 1334, 1588
ScrFI CCNGG 5 cut(s) 149, 163, 310, 1122, 1158
SexAI ACCWGGT 1 cut(s) 147
SfaNI GCATC 4 cut(s) 26, 283, 715, 1464
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 3 cut(s) 1060, 1204, 1283
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SphI GCATGC 1 cut(s) 1129
Sse9I AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
SseBI AGGCCT 1 cut(s) 1312
SsiI CCGC 5 cut(s) 44, 284, 543, 1266, 1345
SspI AATATT 1 cut(s) 186
SspMI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
StuI AGGCCT 1 cut(s) 1312
StyD4I CCNGG 5 cut(s) 147, 161, 308, 1120, 1156
StyI CCWWGG 3 cut(s) 138, 692, 909
TaaI ACNGT 3 cut(s) 36, 1285, 1321
TaqI TCGA 2 cut(s) 70, 120
TasI AATT 7 cut(s) 449, 487, 499, 746, 1103, 1213, 1238
TauI GCSGC 1 cut(s) 46
TfiI GAWTC 3 cut(s) 410, 645, 1027
Tru1I TTAA 4 cut(s) 288, 1307, 1440, 1563
Tru9I TTAA 4 cut(s) 288, 1307, 1440, 1563
TscAI CASTG 2 cut(s) 877, 1506
TseFI GTSAC 2 cut(s) 1326, 1591
TseI GCWGC 4 cut(s) 112, 371, 619, 728
Tsp45I GTSAC 2 cut(s) 1326, 1591
TspDTI ATGAA 7 cut(s) 293, 360, 809, 942, 1022, 1116, 1528
TspGWI ACGGA 2 cut(s) 781, 785
TspRI CASTG 2 cut(s) 877, 1506
Van91I CCANNNNNTGG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 145
XagI CCTNNNNNAGG 1 cut(s) 590
XapI RAATTY 2 cut(s) 487, 746
XceI RCATGY 2 cut(s) 1129, 1209
XmaJI CCTAGG 1 cut(s) 692
XmnI GAANNNNTTC 1 cut(s) 1388
XspI CTAG 6 cut(s) 392, 404, 462, 510, 597, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.