MD05G1235300.v1.1

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002786
Physical Location & Seq
Forward (+)
36724880 .. 36728692
3813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1235300.v1.1.491

Sequence Viewer

Length: 783 bp
ATGCCGCAGGGAGATTACATAGACCTCCACAGGAAGCGGTATGGGTACCGTCCTGACCACTTCGAGCGAAAGCGCAAGAAGGAGGCTCGTCTCGTTCATAAGCGTTCTGAAACTGCTCAGAAGGCTCTGGGTATCAAGGGTAAGATGTTCGCTAAGAAGCGCTATGCTGAAAAGGCTCTCATGAAGAAAACGTTGATTATGCATGAGGAGTCATCGACTAGGCGCAAGGTGGATGATGATGTTCATGAAGGTTCTATTCCAGCATATCTGCTGGATCGTGACACCACAACACGTGCGAAAATTTTGAACAACACTATTAAGCAAAAGCGGAAGGAGAAAGCTGGGAAATGGGAAGTCCCTCTGCCTAAGGTAAGGCCTGTGGCTGAAGATGAGATGTTCAAAATTCTTAGATCTGGGAAACGCAAAACCAAGCAATGGAAGAGGATGGTCACAAAAGCCACATTTGTGGGACCTGGTTTCACAAGAAAACCTCCCAAGTATGAGCGATTTATCCGTCCCAGTGGGTTGCGATTCACCAAATCTCATGTGACACATCCTGAACTTAAAGCCACTTTTAACCTTGAGATCATTGGTGTGAAGAAGAATCCTAACGGTCAAATGTATACATCTCTTGGTGTCTTGACCAAGGGAACCATCATTGAGGTGAATGTCAGTGAACTAGGTCTAGTCACACCTGCCGGGAAAGTTGTATGGGGGAAGTATGCTCAGGTGACAAATAATCCAGAGAACGACGGGTGTGTTAATGCAGTTCTGCTCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

261

Amino Acids

29.84

Weight (kDa)

10.23

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 35 - 259 2.6e-39 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 703
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 435
AccI GTMKAC 1 cut(s) 623
AciI CCGC 3 cut(s) 5, 37, 328
AclI AACGTT 1 cut(s) 191
AclWI GGATC 1 cut(s) 282
AcsI RAATTY 2 cut(s) 300, 402
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 1 cut(s) 293
AfaI GTAC 1 cut(s) 47
AfeI AGCGCT 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 435
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 2 cut(s) 307, 400
AjnI CCWGG 1 cut(s) 472
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 1 cut(s) 282
Aor51HI AGCGCT 1 cut(s) 161
AoxI GGCC 1 cut(s) 374
ApoI RAATTY 2 cut(s) 300, 402
Asp718I GGTACC 1 cut(s) 45
AspLEI GCGC 3 cut(s) 75, 162, 225
AspS9I GGNCC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 700
AsuHPI GGTGA 3 cut(s) 526, 676, 742
AvaII GGWCC 1 cut(s) 470
AxyI CCTNAGG 1 cut(s) 366
BanI GGYRCC 1 cut(s) 45
BbrPI CACGTG 1 cut(s) 293
BccI CCATC 2 cut(s) 439, 662
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 3 cut(s) 219, 680, 686
BfoI RGCGCY 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 703
BglII AGATCT 1 cut(s) 410
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 2 cut(s) 474, 700
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 3 cut(s) 47, 471, 652
BmrFI CCNGG 2 cut(s) 474, 700
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 700
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 645
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 366
Bse3DI GCAATG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 3 cut(s) 238, 450, 553
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 2 cut(s) 131, 740
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 341
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 1 cut(s) 45
BsiSI CCGG 1 cut(s) 699
BslFI GGGAC 3 cut(s) 341, 483, 501
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 1 cut(s) 95
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 3 cut(s) 341, 483, 501
BsnI GGCC 1 cut(s) 376
Bsp143I GATC 3 cut(s) 274, 410, 585
BspACI CCGC 3 cut(s) 5, 37, 328
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 2 cut(s) 130, 739
BspHI TCATGA 2 cut(s) 180, 244
BspLI GGNNCC 3 cut(s) 47, 471, 652
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 440
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 3 cut(s) 274, 410, 585
BssNAI GTATAC 1 cut(s) 624
BssT1I CCWWGG 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 50, 614
Bst6I CTCTTC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 293
BstDEI CTNAG 5 cut(s) 117, 153, 366, 407, 726
BstF5I GGATG 3 cut(s) 238, 450, 553
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 3 cut(s) 75, 162, 225
BstKTI GATC 3 cut(s) 277, 413, 588
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 274, 410, 585
BstMWI GCNNNNNNNGC 2 cut(s) 122, 173
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 472, 698
BstX2I RGATCY 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 410
BstZ17I GTATAC 1 cut(s) 624
Bsu36I CCTNAGG 1 cut(s) 366
BsuRI GGCC 1 cut(s) 376
BtsCI GGATG 3 cut(s) 238, 450, 553
BtsIMutI CAGTG 2 cut(s) 526, 679
BveI ACCTGC 1 cut(s) 703
CciI TCATGA 2 cut(s) 180, 244
CfoI GCGC 3 cut(s) 75, 162, 225
Cfr13I GGNCC 1 cut(s) 470
CsiI ACCWGGT 1 cut(s) 472
Csp6I GTAC 1 cut(s) 46
CviAII CATG 4 cut(s) 181, 203, 245, 545
CviJI RGCY 8 cut(s) 86, 125, 176, 341, 376, 383, 458, 569
CviKI_1 RGCY 8 cut(s) 86, 125, 176, 341, 376, 383, 458, 569
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 5 cut(s) 117, 153, 366, 407, 726
DpnI GATC 3 cut(s) 276, 412, 587
DpnII GATC 3 cut(s) 274, 410, 585
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 470
Eco47III AGCGCT 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 645
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 645
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 4 cut(s) 184, 206, 248, 548
FaqI GGGAC 3 cut(s) 341, 483, 501
FatI CATG 4 cut(s) 180, 202, 244, 544
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 3 cut(s) 245, 457, 540
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 3 cut(s) 219, 680, 686
GlaI GCGC 3 cut(s) 74, 161, 224
GluI GCNGC 1 cut(s) 5
GsaI CCCAGC 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 1 cut(s) 376
HapII CCGG 1 cut(s) 699
HhaI GCGC 3 cut(s) 75, 162, 225
Hin1II CATG 4 cut(s) 184, 206, 248, 548
Hin6I GCGC 3 cut(s) 73, 160, 223
HinP1I GCGC 3 cut(s) 73, 160, 223
HinfI GANTC 3 cut(s) 209, 531, 604
HpaII CCGG 1 cut(s) 699
HphI GGTGA 3 cut(s) 526, 676, 742
Hpy166II GTNNAC 2 cut(s) 624, 677
Hpy188I TCNGA 2 cut(s) 109, 120
Hpy188III TCNNGA 7 cut(s) 53, 181, 245, 278, 557, 640, 743
Hpy8I GTNNAC 2 cut(s) 624, 677
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 4 cut(s) 73, 115, 242, 325
HpyCH4III ACNGT 2 cut(s) 50, 614
HpyCH4IV ACGT 2 cut(s) 191, 292
HpyCH4V TGCA 2 cut(s) 202, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 173
HpyF3I CTNAG 5 cut(s) 117, 153, 366, 407, 726
HpySE526I ACGT 2 cut(s) 191, 292
Hsp92II CATG 4 cut(s) 184, 206, 248, 548
HspAI GCGC 3 cut(s) 73, 160, 223
KpnI GGTACC 1 cut(s) 49
Kzo9I GATC 3 cut(s) 274, 410, 585
MabI ACCWGGT 1 cut(s) 472
MaeI CTAG 3 cut(s) 219, 680, 686
MaeII ACGT 2 cut(s) 191, 292
MaeIII GTNAC 5 cut(s) 278, 448, 547, 688, 730
MalI GATC 3 cut(s) 276, 412, 587
MboI GATC 3 cut(s) 274, 410, 585
MboII GAAGA 5 cut(s) 196, 398, 451, 610, 613
MflI RGATCY 1 cut(s) 410
MluCI AATT 2 cut(s) 300, 402
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 7 cut(s) 35, 76, 199, 369, 435, 501, 655
Mph1103I ATGCAT 1 cut(s) 204
MseI TTAA 5 cut(s) 318, 564, 576, 762, 781
MslI CAYNNNNRTG 3 cut(s) 464, 593, 662
MspI CCGG 1 cut(s) 699
MspR9I CCNGG 2 cut(s) 474, 700
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 2 cut(s) 122, 173
NciI CCSGG 1 cut(s) 700
NdeII GATC 3 cut(s) 274, 410, 585
NlaIII CATG 4 cut(s) 184, 206, 248, 548
NlaIV GGNNCC 3 cut(s) 47, 471, 652
NmuCI GTSAC 5 cut(s) 278, 448, 547, 688, 730
NsiI ATGCAT 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 464
PagI TCATGA 2 cut(s) 180, 244
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 376
PfeI GAWTC 2 cut(s) 531, 604
PflMI CCANNNNNTGG 1 cut(s) 435
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 217
PmaCI CACGTG 1 cut(s) 293
PmlI CACGTG 1 cut(s) 293
PpsI GAGTC 1 cut(s) 217
Ppu21I YACGTR 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 470
Psp1406I AACGTT 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 472
PspCI CACGTG 1 cut(s) 293
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 47, 471, 652
PspPI GGNCC 1 cut(s) 470
PspPPI RGGWCCY 1 cut(s) 470
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 3 cut(s) 464, 593, 662
SaqAI TTAA 5 cut(s) 318, 564, 576, 762, 781
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 274, 410, 585
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 474, 700
SexAI ACCWGGT 1 cut(s) 472
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 3 cut(s) 464, 593, 662
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 2 cut(s) 300, 402
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 3 cut(s) 5, 37, 328
SspMI CTAG 3 cut(s) 219, 680, 686
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 2 cut(s) 472, 698
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 2 cut(s) 50, 614
TaiI ACGT 2 cut(s) 194, 295
TaqI TCGA 2 cut(s) 63, 215
TasI AATT 2 cut(s) 300, 402
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 531, 604
Tru1I TTAA 5 cut(s) 318, 564, 576, 762, 781
Tru9I TTAA 5 cut(s) 318, 564, 576, 762, 781
TscAI CASTG 2 cut(s) 526, 679
TseFI GTSAC 5 cut(s) 278, 448, 547, 688, 730
Tsp45I GTSAC 5 cut(s) 278, 448, 547, 688, 730
TspDTI ATGAA 4 cut(s) 86, 197, 233, 261
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 2 cut(s) 526, 679
Van91I CCANNNNNTGG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 470
XapI RAATTY 2 cut(s) 300, 402
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 3 cut(s) 219, 680, 686
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.